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Yorodumi- PDB-9pgl: In situ structure of the human mitoribosome in the A-P state with... -
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Basic information
| Entry | Database: PDB / ID: 9pgl | ||||||||||||||||||||||||||||||
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| Title | In situ structure of the human mitoribosome in the A-P state with TACO1 | ||||||||||||||||||||||||||||||
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Keywords | RIBOSOME / mitochondria / mitoribosome / inner membrane | ||||||||||||||||||||||||||||||
| Function / homology | Function and homology informationComplex IV assembly / motor learning / mitochondrial protein quality control / protein insertion into mitochondrial inner membrane from matrix / negative regulation of oxidoreductase activity / mitochondrial ribosome binding / membrane insertase activity / regulation of mitochondrial translation / mitochondrial respiratory chain complex IV assembly / Complex I biogenesis ...Complex IV assembly / motor learning / mitochondrial protein quality control / protein insertion into mitochondrial inner membrane from matrix / negative regulation of oxidoreductase activity / mitochondrial ribosome binding / membrane insertase activity / regulation of mitochondrial translation / mitochondrial respiratory chain complex IV assembly / Complex I biogenesis / rRNA import into mitochondrion / mitochondrial transcription / mitochondrial translational termination / negative regulation of ATP-dependent activity / Mitochondrial ribosome-associated quality control / mitochondrial translational elongation / mitochondrial ribosome assembly / Mitochondrial translation elongation / translation release factor activity, codon nonspecific / Mitochondrial translation initiation / mitochondrial proton-transporting ATP synthase complex assembly / Mitochondrial translation termination / negative regulation of mitotic nuclear division / translation release factor activity / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / mitochondrial ribosome / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / mitochondrial small ribosomal subunit / peptidyl-tRNA hydrolase activity / mitochondrial translation / apoptotic mitochondrial changes / mitochondrial respiratory chain complex I assembly / positive regulation of proteolysis / ribosomal small subunit binding / anatomical structure morphogenesis / RNA processing / Mitochondrial protein degradation / rescue of stalled cytosolic ribosome / aerobic respiration / cellular response to leukemia inhibitory factor / protein tetramerization / apoptotic signaling pathway / mitochondrial membrane / fibrillar center / cell junction / double-stranded RNA binding / regulation of translation / ribosomal small subunit assembly / 5S rRNA binding / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / endonuclease activity / nuclear membrane / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / tRNA binding / cell population proliferation / mitochondrial inner membrane / negative regulation of translation / rRNA binding / nuclear body / structural constituent of ribosome / ribosome / translation / mitochondrial matrix / ribonucleoprotein complex / intracellular membrane-bounded organelle / protein domain specific binding / nucleotide binding / mRNA binding / apoptotic process / positive regulation of DNA-templated transcription / GTP binding / nucleolus / protein homodimerization activity / protein-containing complex / mitochondrion / extracellular space / RNA binding / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||||||||||||||||||||||||||
| Biological species | Homo sapiens (human) | ||||||||||||||||||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.9 Å | ||||||||||||||||||||||||||||||
Authors | Wang, S. / Xiong, Y. / Zhang, Y. | ||||||||||||||||||||||||||||||
| Funding support | United States, 1items
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Citation | Journal: Nat Commun / Year: 2026Title: Structural basis of TACO1-mediated efficient mitochondrial translation Authors: Wang, S. / Brischigliaro, M. / Zhang, Y. / Wu, C. / Zheng, W. / Barrientos, A. / Xiong, Y. | ||||||||||||||||||||||||||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9pgl.cif.gz | 4.5 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9pgl.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9pgl.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pg/9pgl ftp://data.pdbj.org/pub/pdb/validation_reports/pg/9pgl | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 71634MC ![]() 9pg8C ![]() 9pgfC ![]() 9pgiC ![]() 9pgmC M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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Components
+39S ribosomal protein ... , 46 types, 48 molecules 0123456789DEFHIJLMNOPQRSTUWXYZ...
-RNA chain , 6 types, 6 molecules AAzAAAxAwB
| #11: RNA chain | Mass: 500727.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: GenBank: 2756414513 |
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| #78: RNA chain | Mass: 10826.415 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) |
| #80: RNA chain | Mass: 306218.312 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: GenBank: 1858621102 |
| #84: RNA chain | Mass: 22664.498 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: GenBank: 1896813686 |
| #85: RNA chain | Mass: 24261.480 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) |
| #90: RNA chain | Mass: 22989.752 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: GenBank: 1896813692 |
-Protein , 7 types, 7 molecules CoqpA3OXA4
| #12: Protein | Mass: 32532.180 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BSH4 |
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| #47: Protein | Mass: 12292.333 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BQC6 |
| #48: Protein | Mass: 25426.895 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TAE8 |
| #52: Protein | Mass: 23674.203 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q14197, peptidyl-tRNA hydrolase |
| #77: Protein | Mass: 22395.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NWT8 |
| #82: Protein | Mass: 48574.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q15070 |
| #86: Protein | Mass: 78648.547 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96EY7 |
-Large ribosomal subunit protein ... , 2 types, 2 molecules Kk
| #19: Protein | Mass: 20617.828 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: H2QWN0 |
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| #43: Protein | Mass: 12020.701 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96EL3 |
-Protein/peptide , 1 types, 1 molecules n
| #46: Protein/peptide | Mass: 3677.524 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) |
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+28S ribosomal protein ... , 25 types, 25 molecules ABACADAEAFAGAHAJAKALAMANAOAPARASATAUAVAWAZA1AYAIAX
-Small ribosomal subunit protein ... , 3 types, 3 molecules A0A2AQ
| #75: Protein | Mass: 25564.354 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82930 |
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| #88: Protein | Mass: 13393.661 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96BP2 |
| #89: Protein | Mass: 10659.494 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82921 |
-Non-polymers , 11 types, 279 molecules 




















| #91: Chemical | | #92: Chemical | ChemComp-K / #93: Chemical | ChemComp-SPD / #94: Chemical | ChemComp-PUT / | #95: Chemical | ChemComp-MG / #96: Chemical | #97: Chemical | ChemComp-NAD / | #98: Chemical | #99: Chemical | ChemComp-ATP / | #100: Chemical | ChemComp-GDP / | #101: Chemical | ChemComp-VAL / | |
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-Details
| Has ligand of interest | Y |
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| Has protein modification | Y |
-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: mitochondria / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1-#16, #18, #20-#34, #17, #35-#42, #44-#87 / Source: NATURAL |
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| Molecular weight | Experimental value: NO |
| Source (natural) | Organism: Homo sapiens (human) |
| Buffer solution | pH: 7.4 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 4000 nm / Nominal defocus min: 1000 nm |
| Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
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| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
| 3D reconstruction | Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 175268 / Symmetry type: POINT | ||||||||||||||||||||||||
| Refine LS restraints |
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About Yorodumi



Homo sapiens (human)
United States, 1items
Citation








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