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- EMDB-7024: Cryo-EM structure of the small subunit of Leishmania ribosome bou... -

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Basic information

Entry
Database: EMDB / ID: EMD-7024
TitleCryo-EM structure of the small subunit of Leishmania ribosome bound to paromomycin
Map dataprimary map
Sample
  • Complex: Leishmania donovani 91S ribosome SSU
    • Protein or peptide: x 33 types
    • RNA: x 5 types
  • Ligand: x 3 types
Function / homology
Function and homology information


translation regulator activity / small-subunit processome / modification-dependent protein catabolic process / protein tag activity / ribosomal small subunit biogenesis / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / rRNA binding ...translation regulator activity / small-subunit processome / modification-dependent protein catabolic process / protein tag activity / ribosomal small subunit biogenesis / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 ...Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / : / Ribosomal protein S6, eukaryotic / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S27 / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S3Ae / Ribosomal protein S28e conserved site / Ribosomal S3Ae family / Ribosomal protein S28e signature. / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal protein S28e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein S14/S29 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like / Ribosomal protein S2 signature 2. / : / KH domain / Type-2 KH domain profile. / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S10 / : / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / Ribosomal protein S5, N-terminal, conserved site
Similarity search - Domain/homology
40S ribosomal protein S5 / Small ribosomal subunit protein uS2 / 40S ribosomal protein S7 / 40S ribosomal protein S21, putative / 40S ribosomal protein S15A, putative / 40S ribosomal protein S12 / 40S ribosomal protein S4 / 40S ribosomal protein S23, putative / 40S ribosomal protein S15, putative / 40S ribosomal protein S8 ...40S ribosomal protein S5 / Small ribosomal subunit protein uS2 / 40S ribosomal protein S7 / 40S ribosomal protein S21, putative / 40S ribosomal protein S15A, putative / 40S ribosomal protein S12 / 40S ribosomal protein S4 / 40S ribosomal protein S23, putative / 40S ribosomal protein S15, putative / 40S ribosomal protein S8 / 40S ribosomal protein S25 / 40S ribosomal protein S16, putative / 40S ribosomal protein S33, putative / Ribosomal protein S10 / Ribosomal protein S29, putative / 40S ribosomal protein S17, putative / 40S ribosomal protein S26 / 40S ribosomal protein S30 / 40S ribosomal protein S14 / 40S ribosomal protein S2 / 40S ribosomal protein S13, putative / 40S ribosomal protein S3 / 40S ribosomal protein S19 protein, putative / Small ribosomal subunit protein eS1 / Ubiquitin-ribosomal protein eL40 fusion protein / 40S ribosomal protein S18, putative / 40S ribosomal protein S10, putative / 40S ribosomal protein S9, putative / 40S ribosomal protein S24 / 40S ribosomal protein S27-1, putative / Small ribosomal subunit protein eS6 / LACK protective antigen / Ribosomal protein S11
Similarity search - Component
Biological speciesLeishmania donovani (eukaryote) / Escherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsShalev-Benami M / Zhang Y / Rozenberg H / Matzov D / Zimmerman E / Bashan A / Jaffe CL / Yonath A / Skiniotis G
CitationJournal: Nat Commun / Year: 2017
Title: Atomic resolution snapshot of Leishmania ribosome inhibition by the aminoglycoside paromomycin.
Authors: Moran Shalev-Benami / Yan Zhang / Haim Rozenberg / Yuko Nobe / Masato Taoka / Donna Matzov / Ella Zimmerman / Anat Bashan / Toshiaki Isobe / Charles L Jaffe / Ada Yonath / Georgios Skiniotis /
Abstract: Leishmania is a single-celled eukaryotic parasite afflicting millions of humans worldwide, with current therapies limited to a poor selection of drugs that mostly target elements in the parasite's ...Leishmania is a single-celled eukaryotic parasite afflicting millions of humans worldwide, with current therapies limited to a poor selection of drugs that mostly target elements in the parasite's cell envelope. Here we determined the atomic resolution electron cryo-microscopy (cryo-EM) structure of the Leishmania ribosome in complex with paromomycin (PAR), a highly potent compound recently approved for treatment of the fatal visceral leishmaniasis (VL). The structure reveals the mechanism by which the drug induces its deleterious effects on the parasite. We further show that PAR interferes with several aspects of cytosolic translation, thus highlighting the cytosolic rather than the mitochondrial ribosome as the primary drug target. The results also highlight unique as well as conserved elements in the PAR-binding pocket that can serve as hotspots for the development of novel therapeutics.
History
DepositionSep 9, 2017-
Header (metadata) releaseDec 6, 2017-
Map releaseDec 6, 2017-
UpdateJul 3, 2019-
Current statusJul 3, 2019Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6az1
  • Surface level: 0.05
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6az1
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_7024.png

Downloads & links

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Map

FileDownload / File: emd_7024.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationprimary map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.02 Å/pix.
x 384 pix.
= 391.68 Å		1.02 Å/pix.
x 384 pix.
= 391.68 Å		1.02 Å/pix.
x 384 pix.
= 391.68 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.02 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.05 / Movie #1: 0.05
Minimum - Maximum-0.232303 - 0.3947016
Average (Standard dev.)0.00026962307 (±0.015102799)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 391.68 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.021.021.02
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z391.680391.680391.680
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.2320.3950.000

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Supplemental data

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Sample components

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Entire : Leishmania donovani 91S ribosome SSU

EntireName: Leishmania donovani 91S ribosome SSU
Components
  • Complex: Leishmania donovani 91S ribosome SSU
    • Protein or peptide: Ribosomal protein s1e
    • Protein or peptide: ribosomal protein S2
    • Protein or peptide: ribosomal protein S3
    • Protein or peptide: ribosomal protein S4
    • Protein or peptide: ribosomal protein S4e
    • Protein or peptide: ribosomal protein S5
    • Protein or peptide: ribosomal protein S6e
    • Protein or peptide: ribosomal protein S7
    • Protein or peptide: ribosomal protein S7e
    • Protein or peptide: ribosomal protein S8
    • Protein or peptide: ribosomal protein S8e
    • Protein or peptide: ribosomal protein S9
    • Protein or peptide: ribosomal protein S10
    • Protein or peptide: ribosomal protein S10e
    • Protein or peptide: ribosomal protein S11
    • Protein or peptide: ribosomal protein S12
    • Protein or peptide: ribosomal protein S12e
    • Protein or peptide: ribosomal protein S13
    • Protein or peptide: ribosomal protein S14
    • Protein or peptide: ribosomal protein S15
    • Protein or peptide: ribosomal protein S17
    • Protein or peptide: ribosomal protein S17e
    • Protein or peptide: ribosomal protein S19
    • Protein or peptide: ribosomal protein S19e
    • Protein or peptide: ribosomal protein S21e
    • Protein or peptide: ribosomal protein S24e
    • Protein or peptide: ribosomal protein S25e
    • Protein or peptide: ribosomal protein S26e
    • Protein or peptide: ribosomal protein S27e
    • Protein or peptide: ribosomal protein S28e
    • Protein or peptide: ribosomal protein S30e
    • Protein or peptide: ribosomal protein S31e
    • Protein or peptide: LACK1
    • RNA: ribosomal RNA 18S
    • RNA: tRNA-Phe
    • RNA: P-site tRNA
    • RNA: E-site tRNA
    • RNA: mRNA
  • Ligand: MAGNESIUM ION
  • Ligand: PAROMOMYCIN
  • Ligand: water

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Supramolecule #1: Leishmania donovani 91S ribosome SSU

SupramoleculeName: Leishmania donovani 91S ribosome SSU / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#38
Source (natural)Organism: Leishmania donovani (eukaryote)

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Macromolecule #1: Ribosomal protein s1e

MacromoleculeName: Ribosomal protein s1e / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 30.092064 KDa
SequenceString: MALGKNKRIS KGGKRSKRGK AQETMARKEW YDVVAPANFE KRQFAKTICN KTQGTRIAAD VLRGRVFEAN LADLNQSAGE EEAYRKVRF TVQEVQGRNL LTQFHSMEVT TDKMASLLRK WCTTMETTVE VKTADGYTMR LFVVAFTKPQ ANQQSRNCYA K QRLVKWLR ...String:
MALGKNKRIS KGGKRSKRGK AQETMARKEW YDVVAPANFE KRQFAKTICN KTQGTRIAAD VLRGRVFEAN LADLNQSAGE EEAYRKVRF TVQEVQGRNL LTQFHSMEVT TDKMASLLRK WCTTMETTVE VKTADGYTMR LFVVAFTKPQ ANQQSRNCYA K QRLVKWLR MRITKMIKRR LSKVQIKEAV SLLTRNVLSD ALVRRCNPIL PLRELRIRKV RVVRTPKFDA QALLSAHGTI PA SVEADQR EVEEAVEAAP AAEKAAE

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Macromolecule #2: ribosomal protein S2

MacromoleculeName: ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 27.564682 KDa
SequenceString: MTAVESGSKV LRMKESDAQK LLAMRCHIGT RNQSSAMKKY IYGRTAEGSH IIDLHMMWEK LILAARVIAA VENPKDVCVC SSRLYGTRA IYKFSQHVGT SFHGGRFIPG TFTNQIQKKF VQPRVLVVTD PRTDHQAIRE ASLVNIPVIA LCDTDAPLEY V DIAIPCNN ...String:
MTAVESGSKV LRMKESDAQK LLAMRCHIGT RNQSSAMKKY IYGRTAEGSH IIDLHMMWEK LILAARVIAA VENPKDVCVC SSRLYGTRA IYKFSQHVGT SFHGGRFIPG TFTNQIQKKF VQPRVLVVTD PRTDHQAIRE ASLVNIPVIA LCDTDAPLEY V DIAIPCNN RGIKSIGMMY WLLAREVLRL RGTIVRSVPW EEKVDLFFYR DPNEAAEEKA AAAAAAPAAE AEEGFGWVER ND DNAWEA

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Macromolecule #3: ribosomal protein S3

MacromoleculeName: ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 24.511762 KDa
SequenceString: MGPLSKKRMI IRDGVFYAEL FEFLKRELAE EGFSGVSYHV TTLRTEIVIK ATKTREVLGV NGRRIRELTA CIQQRFNYKE GKLQLYVER VEVRGLSAMA QVESLRFKLL SNLQVRRAAM GIIRYVMESG AKGCEVTVGG KIKGQRAKSM TFRDGYMIKS G TAHKSFVD ...String:
MGPLSKKRMI IRDGVFYAEL FEFLKRELAE EGFSGVSYHV TTLRTEIVIK ATKTREVLGV NGRRIRELTA CIQQRFNYKE GKLQLYVER VEVRGLSAMA QVESLRFKLL SNLQVRRAAM GIIRYVMESG AKGCEVTVGG KIKGQRAKSM TFRDGYMIKS G TAHKSFVD SACRHCYMRA GCIGVKVKIM LPGDSTGRNG PSEPLPDVIT VIEPKQITAS E

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Macromolecule #4: ribosomal protein S4

MacromoleculeName: ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 22.184914 KDa
SequenceString:
MRNYNNFNRV WKAPRRPFEK ERLDREMKLC GQYGLRCKRE IWRVNMTLSK MRRTARLLLT LPENHPRRLL EGSAIMRRCH EYGFLDEEK DKLDYVLSLT VPDILERRLQ TIVFKAGLAK SVHHARVLIQ QRHIAVAKQI VTIPSFIVRV SSERHIAFAD A SPFGNGRP GRVKRVRAKA AKRHAGGGDD DE

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Macromolecule #5: ribosomal protein S4e

MacromoleculeName: ribosomal protein S4e / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 30.711088 KDa
SequenceString: MAKKHLKRLY APKDWMLSKL TGVFAPRPRP GPHKLRECLP LLVIIRNRLK YALNAREGEM ILRQGLVHVD NHPRRDGKYP AGFMDVVEI PKTGDRFRLM YDVKGRFALV SLSEAEAQIK LMKVVNLYTA TGRVPVAVTH DGHRIRYPDP HTSIGDTIVY N VKEKKCVD ...String:
MAKKHLKRLY APKDWMLSKL TGVFAPRPRP GPHKLRECLP LLVIIRNRLK YALNAREGEM ILRQGLVHVD NHPRRDGKYP AGFMDVVEI PKTGDRFRLM YDVKGRFALV SLSEAEAQIK LMKVVNLYTA TGRVPVAVTH DGHRIRYPDP HTSIGDTIVY N VKEKKCVD LIKNRQGKAV IVTGGANRGR IGEIVKVECH PGAFNIAHLK DASGAEFATR AANIFVIGKD LNHLQVTVPK QQ GLRMNVI QEREERLIAA EARKNAPARG ARKARK

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Macromolecule #6: ribosomal protein S5

MacromoleculeName: ribosomal protein S5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 28.803186 KDa
SequenceString: MADTQPAQEA PAADVPRAER NFGRGRGGRG GRGRGRGGPG EEKEWVPCTK LGRLVKAQKV TSLEEIFLFS MPIKEHQIVD TLIAEGQLH DEMMKIYPVQ KATSAGQRTR FKAFNVVGDC DGHIGIGARV GKEVSLAIRA SMIAAKLNIV PVRRGYWGNK I GEPHTIPM ...String:
MADTQPAQEA PAADVPRAER NFGRGRGGRG GRGRGRGGPG EEKEWVPCTK LGRLVKAQKV TSLEEIFLFS MPIKEHQIVD TLIAEGQLH DEMMKIYPVQ KATSAGQRTR FKAFNVVGDC DGHIGIGARV GKEVSLAIRA SMIAAKLNIV PVRRGYWGNK I GEPHTIPM KVTGKCGSVA VRLVPAPRGT GIVAAPVPKK ILEFAGVEDV YTSSRGKTRT HGNLIMATFY ALRKTYGFLT PD LWAETEP SRDPTDEHAE LLAEMTTA

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Macromolecule #7: ribosomal protein S6e

MacromoleculeName: ribosomal protein S6e / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 28.341164 KDa
SequenceString: MKLNIAYPRN GTVKQFEISD EVLRRVQLQD YRLGNEVDGA IFGSEFKGYI FRLRGGSDKD GFPMVPGVLA SSRVSLLVKR GAIGFNTFR GYQGERRRKN VRGCVLASDI ALVNVTISKV GDQPIEGVTD TTAPRRLGPK RASKIRKLFN LSRTEDVRKY V VRRRVVKS ...String:
MKLNIAYPRN GTVKQFEISD EVLRRVQLQD YRLGNEVDGA IFGSEFKGYI FRLRGGSDKD GFPMVPGVLA SSRVSLLVKR GAIGFNTFR GYQGERRRKN VRGCVLASDI ALVNVTISKV GDQPIEGVTD TTAPRRLGPK RASKIRKLFN LSRTEDVRKY V VRRRVVKS GKKDRLKAPK IQRLITPRVK ARRAKKAKDA IAKVRASAAE RREYLRLIAS NRRALRQRDH SKKHTQKVHA QR AEVAAFQ KK

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Macromolecule #8: ribosomal protein S7

MacromoleculeName: ribosomal protein S7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 21.311596 KDa
SequenceString:
MSSKTPKLFN KWSFEGLETS ELALRDHIST TAAYVPHTSG RWQKRRFHKA RMPIVERLAN GLMFKGRGNG RKLQAARLLK HTLEIIHLL TDENPLQVVV DAVSKGAPRE DSTRVGSGGV VRRQAVDVSP MRRVNEAIYQ MCKGAREAAF RNLKSMPECL A DEIVNASK GSSNSYAIKK KDEVERVAKA NR

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Macromolecule #9: ribosomal protein S7e

MacromoleculeName: ribosomal protein S7e / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 23.867316 KDa
SequenceString: MQPYLRKLRK LKRGKPSDLE ESVAKALFEL ETSHKTLRQE LPRFHVNTVR EVKVPRSKKT VLVVFYPLRF LMLVRKVQRA LTSELEKRH PGYLVMLVAQ RKITKRPTDV YKLQKVQRSK TSTAVFENIL NDMIYPSDVV GRRWRCRTDG SKLMKVFLDA R DRKRVESR ...String:
MQPYLRKLRK LKRGKPSDLE ESVAKALFEL ETSHKTLRQE LPRFHVNTVR EVKVPRSKKT VLVVFYPLRF LMLVRKVQRA LTSELEKRH PGYLVMLVAQ RKITKRPTDV YKLQKVQRSK TSTAVFENIL NDMIYPSDVV GRRWRCRTDG SKLMKVFLDA R DRKRVESR LRAVAYVYKQ LTHRRVSFGF MWNPKLQQVS TR

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Macromolecule #10: ribosomal protein S8

MacromoleculeName: ribosomal protein S8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 14.719258 KDa
SequenceString:
MTMMSVLANA LRTIASAERR GKRQVLIRPS SKVVVKFLQV MQKHGYIGEF EIIDDHRAGK IVVNLNGRLN KCGAICPRFD CATTDYEKW MKNILPSRQF GFVVLTTSLG IMDHEEARSR NTGGKVLGFF Y

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Macromolecule #11: ribosomal protein S8e

MacromoleculeName: ribosomal protein S8e / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 25.038346 KDa
SequenceString: MGIVRSRLHK RKITGGKTKI HRKRMKAELG RLPANTRLGA RRVSPVRARG GNFKIRALRL DTGNFAWASE AIAHRVRLLD VVYNATSNE LVRTKTLVKN CIVAVDAAPF KRWYAKHYGI DLDADRKTTK AAAAAEKKGR KSVHAAADKY DVNKASPKLQ R EWIRRRRN ...String:
MGIVRSRLHK RKITGGKTKI HRKRMKAELG RLPANTRLGA RRVSPVRARG GNFKIRALRL DTGNFAWASE AIAHRVRLLD VVYNATSNE LVRTKTLVKN CIVAVDAAPF KRWYAKHYGI DLDADRKTTK AAAAAEKKGR KSVHAAADKY DVNKASPKLQ R EWIRRRRN HRVEKAIADQ LREGRVLARI TSRPGQSGRA DGILLEGAEL QFYLKRLEKK KK

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Macromolecule #12: ribosomal protein S9

MacromoleculeName: ribosomal protein S9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 16.718586 KDa
SequenceString:
MPADKSYALK QVQTFGKKKT AIAVATVTKA AQCNIKVNGV PLQQILPDTL RAKIMEAITV VGSKYYSRLR IDVAVHGGGQ VSQAYAARQ AIAKGLIAFF QKYHNEVEKA ALKDKFLAYD KFLLIADPRR CEPKKWGRHS ARTRFTKSYR

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Macromolecule #13: ribosomal protein S10

MacromoleculeName: ribosomal protein S10 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 13.049076 KDa
SequenceString:
MDYPKKTQAA PAEGQTVRLT ITSRNAKAVE SVTSQLLMRA RDEKVTIHGP VRLPTRTLKI TTRKTPCGNG TNTWDTFELK IYKRIIDLH APTEQVKKIT SFTMESGVDV SITILDR

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Macromolecule #14: ribosomal protein S10e

MacromoleculeName: ribosomal protein S10e / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.43291 KDa
SequenceString:
MWCARTRTGS HSHHPTQPGS PIDCAEAAPR RMSTYVPKPS RDNVYRFFFT EGVIACKKDR MGTWTGTLGG NTFTVPCIQV MQLMRSLKS RNLIKEQYAW RHYYWTLNDE GIAYMRSYLH LAPSAMPNTQ KPSSVNFEKV TEGRGRGRGR GGRG

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Macromolecule #15: ribosomal protein S11

MacromoleculeName: ribosomal protein S11 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 15.609088 KDa
SequenceString:
MSKKQEVKAY GPNVKKGDLV YGVVHIYASF NDTFVHVTDT SGRETYVKVT GGMKVKADRD ESSPYAAMMA AQDVVARCKE CGINALHVK MRAVGGVRTK SPGPGAQAAL RALARAGMKI GRIEDVTPIP TDSTRRKGSR RGRRL

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Macromolecule #16: ribosomal protein S12

MacromoleculeName: ribosomal protein S12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 15.952654 KDa
SequenceString:
MTKTNGQNAA RKLVRLRRRN RWADKGWKRA HTFAAKKANP FGGSSHAKGI VLEKIGVGAK QPNSAIRKCV RVQLIKNDKK IIAFVPNDG CLHFIEENDE VLVSGFGRSG HAVGDIPGVR FKIVKVSSVG LYALYRQKKE KPRN

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Macromolecule #17: ribosomal protein S12e

MacromoleculeName: ribosomal protein S12e / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 15.611837 KDa
SequenceString:
MAEETVRVEV PAVEENVVVD VAPESLEDAV RIVIQKALEA NGLVRGLSEV ARTLDCKTAH MCILADDCED EEYKKLVTAL AKQGNIDLI NVEEREKLAQ WAGLVRRDVA GEVTKTLKCS SVAIRDFGER TKALDYLLSQ LH

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Macromolecule #18: ribosomal protein S13

MacromoleculeName: ribosomal protein S13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.443436 KDa
SequenceString:
MSLTLIPDHF QHIVRLLNTN VEGKRKVPFA LRMVKGVGIR FAYLVCKKAG IDVERRAGTL TAEELEKIAE IIADPAKFKI PDWFLNRQR DPKTGKTEHL SSSMVDTRLR EDLERLKKIR AHRGVRHAYG LRVRGQHTCT TGRHGKTVGV SRGK

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Macromolecule #19: ribosomal protein S14

MacromoleculeName: ribosomal protein S14 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 6.696929 KDa
SequenceString:
MGHLDQWRSR QKIGMGKGAR CCVICSNQKA LIRKYELNVC RQCFRENAEH IGFTKLR

+
Macromolecule #20: ribosomal protein S15

MacromoleculeName: ribosomal protein S15 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.45574 KDa
SequenceString:
MVRMHGNGRG KASSALPYRR TPPAWLKIAS RNVVKMVCKS SRKGMMPSQI GMELRDSMGI AQVKNVTGRK ILRILKHSGL APEIPEDLY FLVKRATQMR KHLERHTTDR DTKYRLILVE SRIHRLARYY KRVKQLPPTW KYESSTASAM VA

+
Macromolecule #21: ribosomal protein S17

MacromoleculeName: ribosomal protein S17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 19.985369 KDa
SequenceString:
MSAAPQYYHA HQVDATIQHQ KAYQRQTAVN ENMHRSSRKH VNKSGHIRYA KKIGLGFKTP AKALNGKYID RKCPFTSNVV IRGRILRGI VHSTKMHRTI VIRRNYLHFI KKYQRYQKRH KSLAVHCSPA FDPKQGDEVV VGQCRPLSKT IRYNVLEVVS K SAADKMGK KFAKN

+
Macromolecule #22: ribosomal protein S17e

MacromoleculeName: ribosomal protein S17e / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 16.521465 KDa
SequenceString:
MGKIRTKTVK RASRQVVEKY YSKLNFDFYQ NKRVIMDVTI AESKKLKNKI AGYTTHIMKR LARGPVRGIS LKLQEEERER RMDHAPATS DVDKAIQSGV SVDKRTMQML QRLEIGVPRR VKRADAAVAK VKAAPRRRQQ KSSK

+
Macromolecule #23: ribosomal protein S19

MacromoleculeName: ribosomal protein S19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.528463 KDa
SequenceString:
MASNITAERY EQLKKERTFH KFTYRGLEID PLLALSEEEF RALVHARARR NMNRHVDRRP PVLLKRLREA KKHVKVGEKP KAVKTHLRD VVITPEMVGS VVAIYNGHQF NAVEIKGEMI GHYLGEFSMS YRPVLHGRPG VGATHSSRFI PIK

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Macromolecule #24: ribosomal protein S19e

MacromoleculeName: ribosomal protein S19e / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 20.188588 KDa
SequenceString:
MYSPARMSFI RRVTNPSCIM AALKNKIHRI GKRKGASLKD VSAWRWIKTA ARHFKQEGKI FVPNCTEIMK SSHGRERAPQ NPDWYYIRC AAVLRAIYLR PGVGYGGLSK RFGNKKNYGS RPEHTVTSST GPLHWACKSL TKLGLVEPGA QSGQRLTRKG H KFADSLAF QVQIRKFGAS K

+
Macromolecule #25: ribosomal protein S21e

MacromoleculeName: ribosomal protein S21e / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 16.669115 KDa
SequenceString:
MATIGMFNEE GENVDLYIPR KCHATNTLIE AHDHAAVQIS IANVGPNGVI NGTTTTLCIA GYLRSQGESD HAINHITIDR GIMRIKTGK PKRVSKSKSK KPTAGAAGQK GARPPAQKGA RPSGQKGARP PAQKGARPPA QKGARPPAQK GAAPARKNRA

+
Macromolecule #26: ribosomal protein S24e

MacromoleculeName: ribosomal protein S24e / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 15.808882 KDa
SequenceString:
MVFQKKKAEV CIRTSQFKVN KLLNRKQFIV EVNHPHWCGT VPTQLIRKKL ATLYKVPDAS QVSLFGFKTK FGGGKTTGFG LIYDDLASL KRFEPNYRKT RMGFGKARLP ARKSVKERRN RNKKLRGKAK GKQVAKKK

+
Macromolecule #27: ribosomal protein S25e

MacromoleculeName: ribosomal protein S25e / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 13.071399 KDa
SequenceString:
MPPKAGQTKK AKMEAANKGA KKTTKKWSKG QSREALQNAV MFDKETYDKL RSEVPKYKLI TPSIISDRLK IAVSIAAAGL KQLCREKLI RLVSCSSKTR VYTRIVQAAP AEAAAAAPAA E

+
Macromolecule #28: ribosomal protein S26e

MacromoleculeName: ribosomal protein S26e / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 12.794251 KDa
SequenceString:
MTTKRRNHGR SKPAHSRGRV KPIHCFNCGR LTPKDKAVGR FVVRRMLDAA SARDVAEASP VYGANFPMPK LYMKQRFCIA CAIHSRTVR ARPVGNRKIR YTRKVPFRPA GKK

+
Macromolecule #29: ribosomal protein S27e

MacromoleculeName: ribosomal protein S27e / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 9.819463 KDa
SequenceString:
MGFFDADLSY PTVRTERMKH KRRRLVQGPN SYFMDVSCPR CRQVTVVYSH ATTSVECKGC SKKLCRPTGG KALLVEGCGY RRKPDH

+
Macromolecule #30: ribosomal protein S28e

MacromoleculeName: ribosomal protein S28e / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 9.763191 KDa
SequenceString:
MADSKKDNKK TEEVVTQGTD QAQVGLIIKV LGRTGSRGNV TQVRVRLMAE AGSPDYNRTI VRNVKGPCKE NDMLSLMETE REARRLR

+
Macromolecule #31: ribosomal protein S30e

MacromoleculeName: ribosomal protein S30e / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 7.394894 KDa
SequenceString:
MGKIHGSLAR AGKVKNQTPK VAKQEKPKQP RGRALKRLKY TRRFLSKTVK PGEKVHMNKQ PPGKAG

+
Macromolecule #32: ribosomal protein S31e

MacromoleculeName: ribosomal protein S31e / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 17.015727 KDa
SequenceString:
MQIFIKNAAG RSVAVRVSAE DTVASLKAQA NVTQGNLFFA GMCLAEEETL AAYGLSKEST VDVVIPVEGG KGKKKKKRIF TKPKKPTHR HKLEKMRALK YFKVTENDDG SYKVERTRQD CPHPQCGAGV YMAQHKDRQY CGKCHLTYKA ESK

+
Macromolecule #33: LACK1

MacromoleculeName: LACK1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 34.459523 KDa
SequenceString: MNYEGHLRGH RGWVTSLACP EQAESYIKVV STSRDGTAIA WKANADRHSV DSDYGLPSHR LEGHTGFVSC VSLAHATDYA LTASWDRSI RMWDLRNGQS QRKFLKHTKD VLAVAFSPDD RLIVSAGRDN VVRVWNVAGD CMHEFLRDGH KDWVSSICFS P SLEHPIVV ...String:
MNYEGHLRGH RGWVTSLACP EQAESYIKVV STSRDGTAIA WKANADRHSV DSDYGLPSHR LEGHTGFVSC VSLAHATDYA LTASWDRSI RMWDLRNGQS QRKFLKHTKD VLAVAFSPDD RLIVSAGRDN VVRVWNVAGD CMHEFLRDGH KDWVSSICFS P SLEHPIVV SGSWDNTIKV WNVNGGKCER TLKGHSNYVS TVTVSPDGSL CASGGKDGVA LLWDLSTGEQ LFKINVESPI NQ IAFSPNR FWMCVATEKS LSVYDLESKT VIAELTPDGA KPSECISIAW SADGNTLYSG HKDNLIRVWS ISDAE

+
Macromolecule #34: ribosomal RNA 18S

MacromoleculeName: ribosomal RNA 18S / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 710.287438 KDa
SequenceString: GAUCUGG(OMU)UG AUUCUGC(OMC)AG UAGUCAU(A2M)UG CU(OMU)GUUU(OMC)AA GGACUUAGCC AUGCAUGCCU CAGAAUCAC UGCAUUUGCA GGAAUCUGCG CAUGGCUC(A2M)U UACAUCAGAC GUAAU(OMC)UGCC GCAAAAAUCU UGC GGUUUC ...String:
GAUCUGG(OMU)UG AUUCUGC(OMC)AG UAGUCAU(A2M)UG CU(OMU)GUUU(OMC)AA GGACUUAGCC AUGCAUGCCU CAGAAUCAC UGCAUUUGCA GGAAUCUGCG CAUGGCUC(A2M)U UACAUCAGAC GUAAU(OMC)UGCC GCAAAAAUCU UGC GGUUUC CGCAAAAUUG GAUAACUUGG CGAAACGCCA AGCUAAUACA UGAACCAACC GGGUGUUCUC CACUCCAGAC GGUG GGCAA CCAUCGUCGU GAGACGCCCA GCGAAUGAAU GACAGUAAAA CCAAUGCCUU CACUGGCAGU AACACCCAGC AGUGU UGAC UCAAUUCAUU CCGUGCGAAA GCCGGCUUGU UCCGGCGUCU UUUGACGAAC AACUGCCCUA UCAGCUGGUG AUGGCC GUG UAGUGGACUG CCAUGGCGUU GACGGGAGCG GGGGAUUAGG GUUCGAUUCC GGAGAGGGAG CCUGAGAAAU AGCUACC AC UUCUACGGAG GGCAGCAGGC GCGC(A2M)AAUUG CCCAAUGUCA AAACAAAACG AUGA(OMG)GCAGC GAAAAGAAAU AGAGUUGUC AGUCCAUUUG GAUUGUCAUU UCAAUGGGGG AUAUUUAAAC CCAUCCAAUA UCGAGUAACA AUUGGAGGAC A AGUCUGGU GCCAGCACCC GCGGUAAUUC CAGCUCCAAA AGCGUAUAUU AAUGCUGU(OMU)G CUGUU(A2M)AAGG GUUC GUAGU UGAACUGUGG GCUGUGCAGG UUUGUUCCUG GUCGUCCCGU CCAUGUCGGA UUUGGUGACC CAGGCCCUUG CAGCC CGUG AACAUUCAAA GAAACAAGAA ACACGGGAGU GGUUCCUUUC CUGAUUUACG CAUGUCAUGC AUGCCAGGGG GCGUCC GUG AUUUUUUACU GUGACUAAAG AAGCGUGACU AAAGCAGUCA UUUGACUUGA AUUAGAAAGC AUGGGAUAAC AA(A2M) GGAGCA GCCUCUAGGC UACCGUUUCG GCUUUUGUUG GUUUUAAAGG UCUAUUGGAG AUUAUGGAGC UGUGCGACAA GUGC UUUCC CAUCGCAACC UCGGUUCGGU GUGUGGCGCC UUUGAGGGGU UUAGUGCGUC CGGUACGAGC UCCGGUUCGU CCGGC CGUA ACGCCUUUUC AACUCACGGC CUCUAGGAAU GAAGGAGGGU AGUUCGGGGG AGAACGUACU GGGGCGUCAG AGGUGA AAU UCUUAGACCG CACCAAGACG AACUACAGCG AAGGCAUUCU UCAAGGAUAC CUUCCUCAAU CAAGAACCAA AGUGUGG AG AUCGAAGAUG AUUAGAGACC AUUGUAGUCC ACACUGCAAA CGAUGACACC CAUGAAUUGG GGAUCUUAUG GGCCGGCC U GCGGCAGGGU UUACCCUGUG UCAGCACCGC GCCCGCUUUU ACCACCUUAC GUAUCUUUUC UAUUCGGCCU UUACCGGCC ACCCACGGGA AUAUCCUCAG CACGUUUUCU GUUUUUUCAC GCGAAAGCUU UGAGGUUACA GUCUCAGGGG G(OMG)AGUA CGU UCGCAA(OMG)AGU GAAACUUAAA GAAAUUGACG GAAUGGCACC ACAAGACGUG GAGCGUGCGG UUUAAUU(M1Y)G AC(C4J)(5MC)AACAC(OMG) GGGAACUUUA CCAGAUCCGG ACAGGAUGAG GAUUGACAGA UUGAGUGUUC UUUCUCGA U UCCCUGAAUG G(OMU)G(OMG)UGCAUG GCCGCUUUUG GUCGGUG(OMG)AG UGAUUUGUUU GGUUGAUUCC GUCAACG GA CGAGAUCCAA GCUGCCCAGU AGAAUUCAGA AUUGCCCAUA GGAUAGCAAA CUCAUCGGCG GGUUUUACCC AACGGUGG G CCGCAUUCGG UCGAAUUCU(OMU) CUCUGCGGGA UUCCUUUGUA AUUGCACAAG GUGAAAUUUU GGGCAACAGC A (OMG)GUC(OMU)GUG AUGCUCCUCA AUGUUCUGGG CGACACGC(OMG)(OMC) ACUACAAUGU CAGUGAGAAC AAGAAA AAC GACUUUUGUC GAACCUACUU GAUCAAAAGA GUGGGGAAAC CCCGGAAUCA CAUAGACUCA CUUGGGACCG AGGAUUG CA AUUA(OMU)UGGUC GCGCAACGAG (7MG)AAUGUCUCG UAGGCGCAGC UCAUCA(A2M)ACU GUGCCGAUUA CGUCCC UGC CAUU(OMU)GUACA CACCG(4OC)C(5MC)GU CGUUGUUUCC GAUGAUGGUG CAAUACAGGU GAUCGGACAG GCGGU GUUU UAUCCGCCCG AAAGUUCACC GAUAUUU(OMC)UU CAAUAGAG(OMG)A AGCAAAAGUC GUAACAAGGU AGCUGUAG G UG(MA6)(MA6)CCUGCA GCUGGAUCAU UU

+
Macromolecule #35: tRNA-Phe

MacromoleculeName: tRNA-Phe / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.599748 KDa
SequenceString:
GCGCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAA(MIA)AUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGC GCACCA

+
Macromolecule #36: P-site tRNA

MacromoleculeName: P-site tRNA / type: rna / ID: 36 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.786785 KDa
SequenceString:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGAUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

+
Macromolecule #37: E-site tRNA

MacromoleculeName: E-site tRNA / type: rna / ID: 37 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.485539 KDa
SequenceString:
GCGCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGC GCACCA

+
Macromolecule #38: mRNA

MacromoleculeName: mRNA / type: rna / ID: 38 / Number of copies: 1
Source (natural)Organism: Leishmania donovani (eukaryote)
Molecular weightTheoretical: 4.085501 KDa
SequenceString:
CACCAUGUUC AAA

+
Macromolecule #39: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 39 / Number of copies: 24 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #40: PAROMOMYCIN

MacromoleculeName: PAROMOMYCIN / type: ligand / ID: 40 / Number of copies: 6 / Formula: PAR
Molecular weightTheoretical: 615.628 Da
Chemical component information

ChemComp-PAR:
PAROMOMYCIN / Antimicrobial, medication*YM

+
Macromolecule #41: water

MacromoleculeName: water / type: ligand / ID: 41 / Number of copies: 262 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 1.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 141028
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

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