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- PDB-4v95: Crystal structure of YAEJ bound to the 70S ribosome -

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Basic information

Entry
Database: PDB / ID: 4v95
TitleCrystal structure of YAEJ bound to the 70S ribosome
Components
  • (30S Ribosomal Protein ...) x 20
  • (50S Ribosomal Protein ...) x 29
  • 16S Ribosomal RNA
  • 23S Ribosomal RNA
  • 5S Ribosomal RNA
  • P-site fMet-tRNA
  • YAEJ
  • mRNA
KeywordsRIBOSOME / YaeJ / Ribosome Stalling / Ribosome Rescue / Rescue Factor / Alternative Rescue Factor / ArfB / Release Factor / Rescue of Stalled Ribosomes
Function / homology
Function and homology information


translation release factor activity / peptidyl-tRNA hydrolase / peptidyl-tRNA hydrolase activity / rescue of stalled ribosome / large ribosomal subunit / ribosome binding / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit ...translation release factor activity / peptidyl-tRNA hydrolase / peptidyl-tRNA hydrolase activity / rescue of stalled ribosome / large ribosomal subunit / ribosome binding / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal ...Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / : / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L36 / Ribosomal protein S11, bacterial-type / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal protein S18, conserved site / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18 signature. / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L27
Similarity search - Domain/homology
: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 ...: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Peptidyl-tRNA hydrolase ArfB / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL29 / 30S ribosomal protein S17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / 30S ribosomal protein S14 type Z / 30S ribosomal protein S8 / 50S ribosomal protein L6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bTHX / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL17
Similarity search - Component
Biological speciesEscherichia coli K-12 (bacteria)
Thermus thermophilus (bacteria)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.2 Å
AuthorsGagnon, M.G. / Seetharaman, S.V. / Bulkley, D.P. / Steitz, T.A.
CitationJournal: Science / Year: 2012
Title: Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.
Authors: Gagnon, M.G. / Seetharaman, S.V. / Bulkley, D. / Steitz, T.A.
History
DepositionJan 27, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 9, 2014Provider: repository / Type: Initial release
SupersessionDec 10, 2014ID: 4DH9, 4DHA, 4DHB, 4DHC
Revision 1.1Dec 10, 2014Group: Other
Revision 1.2Jun 27, 2018Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Source and taxonomy
Category: entity_src_nat / pdbx_entity_src_syn ...entity_src_nat / pdbx_entity_src_syn / pdbx_struct_conn_angle / struct_conn / struct_ref_seq
Item: _entity_src_nat.pdbx_ncbi_taxonomy_id / _pdbx_entity_src_syn.ncbi_taxonomy_id ..._entity_src_nat.pdbx_ncbi_taxonomy_id / _pdbx_entity_src_syn.ncbi_taxonomy_id / _pdbx_entity_src_syn.organism_scientific / _struct_ref_seq.db_align_beg / _struct_ref_seq.db_align_end
Revision 1.3Jul 11, 2018Group: Data collection / Source and taxonomy / Category: entity_src_gen
Item: _entity_src_gen.gene_src_strain / _entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id / _entity_src_gen.pdbx_gene_src_scientific_name
Revision 1.4Nov 6, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / pdbx_struct_conn_angle / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_PDB_ins_code / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_PDB_ins_code / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_ptnr1_PDB_ins_code / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
AA: 16S Ribosomal RNA
AB: 30S Ribosomal Protein S2
AC: 30S Ribosomal Protein S3
AD: 30S Ribosomal Protein S4
AE: 30S Ribosomal Protein S5
AF: 30S Ribosomal Protein S6
AG: 30S Ribosomal Protein S7
AH: 30S Ribosomal Protein S8
AI: 30S Ribosomal Protein S9
AJ: 30S Ribosomal Protein S10
AK: 30S Ribosomal Protein S11
AL: 30S Ribosomal Protein S12
AM: 30S Ribosomal Protein S13
AN: 30S Ribosomal Protein S14
AO: 30S Ribosomal Protein S15
AP: 30S Ribosomal Protein S16
AQ: 30S Ribosomal Protein S17
AR: 30S Ribosomal Protein S18
AS: 30S Ribosomal Protein S19
AT: 30S Ribosomal Protein S20
AU: 30S Ribosomal Protein THX
AY: YAEJ
AV: P-site fMet-tRNA
AX: mRNA
BA: 23S Ribosomal RNA
BB: 5S Ribosomal RNA
BD: 50S Ribosomal Protein L2
BE: 50S Ribosomal Protein L3
BF: 50S Ribosomal Protein L4
BG: 50S Ribosomal Protein L5
BH: 50S Ribosomal Protein L6
BI: 50S Ribosomal Protein L9
BN: 50S Ribosomal Protein L13
BO: 50S Ribosomal Protein L14
BP: 50S Ribosomal Protein L15
BQ: 50S Ribosomal Protein L16
BR: 50S Ribosomal Protein L17
BS: 50S Ribosomal Protein L18
BT: 50S Ribosomal Protein L19
BU: 50S Ribosomal Protein L20
BV: 50S Ribosomal Protein L21
BW: 50S Ribosomal Protein L22
BX: 50S Ribosomal Protein L23
BY: 50S Ribosomal Protein L24
BZ: 50S Ribosomal Protein L25
B0: 50S Ribosomal Protein L27
B1: 50S Ribosomal Protein L28
B2: 50S Ribosomal Protein L29
B3: 50S Ribosomal Protein L30
B4: 50S Ribosomal Protein L31
B5: 50S Ribosomal Protein L32
B6: 50S Ribosomal Protein L33
B7: 50S Ribosomal Protein L34
B8: 50S Ribosomal Protein L35
B9: 50S Ribosomal Protein L36
CA: 16S Ribosomal RNA
CB: 30S Ribosomal Protein S2
CC: 30S Ribosomal Protein S3
CD: 30S Ribosomal Protein S4
CE: 30S Ribosomal Protein S5
CF: 30S Ribosomal Protein S6
CG: 30S Ribosomal Protein S7
CH: 30S Ribosomal Protein S8
CI: 30S Ribosomal Protein S9
CJ: 30S Ribosomal Protein S10
CK: 30S Ribosomal Protein S11
CL: 30S Ribosomal Protein S12
CM: 30S Ribosomal Protein S13
CN: 30S Ribosomal Protein S14
CO: 30S Ribosomal Protein S15
CP: 30S Ribosomal Protein S16
CQ: 30S Ribosomal Protein S17
CR: 30S Ribosomal Protein S18
CS: 30S Ribosomal Protein S19
CT: 30S Ribosomal Protein S20
CU: 30S Ribosomal Protein THX
CV: P-site fMet-tRNA
CX: mRNA
DA: 23S Ribosomal RNA
DB: 5S Ribosomal RNA
DD: 50S Ribosomal Protein L2
DE: 50S Ribosomal Protein L3
DF: 50S Ribosomal Protein L4
DG: 50S Ribosomal Protein L5
DH: 50S Ribosomal Protein L6
DI: 50S Ribosomal Protein L9
DN: 50S Ribosomal Protein L13
DO: 50S Ribosomal Protein L14
DP: 50S Ribosomal Protein L15
DQ: 50S Ribosomal Protein L16
DR: 50S Ribosomal Protein L17
DS: 50S Ribosomal Protein L18
DT: 50S Ribosomal Protein L19
DU: 50S Ribosomal Protein L20
DV: 50S Ribosomal Protein L21
DW: 50S Ribosomal Protein L22
DX: 50S Ribosomal Protein L23
DY: 50S Ribosomal Protein L24
DZ: 50S Ribosomal Protein L25
D0: 50S Ribosomal Protein L27
D1: 50S Ribosomal Protein L28
D2: 50S Ribosomal Protein L29
D3: 50S Ribosomal Protein L30
D4: 50S Ribosomal Protein L31
D5: 50S Ribosomal Protein L32
D6: 50S Ribosomal Protein L33
D7: 50S Ribosomal Protein L34
D8: 50S Ribosomal Protein L35
D9: 50S Ribosomal Protein L36
hetero molecules


Theoretical massNumber of molelcules
Total (without water)4,470,8342459
Polymers4,413,142109
Non-polymers57,6922350
Water63,3773518
1
AA: 16S Ribosomal RNA
AB: 30S Ribosomal Protein S2
AC: 30S Ribosomal Protein S3
AD: 30S Ribosomal Protein S4
AE: 30S Ribosomal Protein S5
AF: 30S Ribosomal Protein S6
AG: 30S Ribosomal Protein S7
AH: 30S Ribosomal Protein S8
AI: 30S Ribosomal Protein S9
AJ: 30S Ribosomal Protein S10
AK: 30S Ribosomal Protein S11
AL: 30S Ribosomal Protein S12
AM: 30S Ribosomal Protein S13
AN: 30S Ribosomal Protein S14
AO: 30S Ribosomal Protein S15
AP: 30S Ribosomal Protein S16
AQ: 30S Ribosomal Protein S17
AR: 30S Ribosomal Protein S18
AS: 30S Ribosomal Protein S19
AT: 30S Ribosomal Protein S20
AU: 30S Ribosomal Protein THX
AY: YAEJ
AV: P-site fMet-tRNA
AX: mRNA
BA: 23S Ribosomal RNA
BB: 5S Ribosomal RNA
BD: 50S Ribosomal Protein L2
BE: 50S Ribosomal Protein L3
BF: 50S Ribosomal Protein L4
BG: 50S Ribosomal Protein L5
BH: 50S Ribosomal Protein L6
BI: 50S Ribosomal Protein L9
BN: 50S Ribosomal Protein L13
BO: 50S Ribosomal Protein L14
BP: 50S Ribosomal Protein L15
BQ: 50S Ribosomal Protein L16
BR: 50S Ribosomal Protein L17
BS: 50S Ribosomal Protein L18
BT: 50S Ribosomal Protein L19
BU: 50S Ribosomal Protein L20
BV: 50S Ribosomal Protein L21
BW: 50S Ribosomal Protein L22
BX: 50S Ribosomal Protein L23
BY: 50S Ribosomal Protein L24
BZ: 50S Ribosomal Protein L25
B0: 50S Ribosomal Protein L27
B1: 50S Ribosomal Protein L28
B2: 50S Ribosomal Protein L29
B3: 50S Ribosomal Protein L30
B4: 50S Ribosomal Protein L31
B5: 50S Ribosomal Protein L32
B6: 50S Ribosomal Protein L33
B7: 50S Ribosomal Protein L34
B8: 50S Ribosomal Protein L35
B9: 50S Ribosomal Protein L36
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,247,8871421
Polymers2,214,39955
Non-polymers33,4881366
Water64936
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
CA: 16S Ribosomal RNA
CB: 30S Ribosomal Protein S2
CC: 30S Ribosomal Protein S3
CD: 30S Ribosomal Protein S4
CE: 30S Ribosomal Protein S5
CF: 30S Ribosomal Protein S6
CG: 30S Ribosomal Protein S7
CH: 30S Ribosomal Protein S8
CI: 30S Ribosomal Protein S9
CJ: 30S Ribosomal Protein S10
CK: 30S Ribosomal Protein S11
CL: 30S Ribosomal Protein S12
CM: 30S Ribosomal Protein S13
CN: 30S Ribosomal Protein S14
CO: 30S Ribosomal Protein S15
CP: 30S Ribosomal Protein S16
CQ: 30S Ribosomal Protein S17
CR: 30S Ribosomal Protein S18
CS: 30S Ribosomal Protein S19
CT: 30S Ribosomal Protein S20
CU: 30S Ribosomal Protein THX
CV: P-site fMet-tRNA
CX: mRNA
DA: 23S Ribosomal RNA
DB: 5S Ribosomal RNA
DD: 50S Ribosomal Protein L2
DE: 50S Ribosomal Protein L3
DF: 50S Ribosomal Protein L4
DG: 50S Ribosomal Protein L5
DH: 50S Ribosomal Protein L6
DI: 50S Ribosomal Protein L9
DN: 50S Ribosomal Protein L13
DO: 50S Ribosomal Protein L14
DP: 50S Ribosomal Protein L15
DQ: 50S Ribosomal Protein L16
DR: 50S Ribosomal Protein L17
DS: 50S Ribosomal Protein L18
DT: 50S Ribosomal Protein L19
DU: 50S Ribosomal Protein L20
DV: 50S Ribosomal Protein L21
DW: 50S Ribosomal Protein L22
DX: 50S Ribosomal Protein L23
DY: 50S Ribosomal Protein L24
DZ: 50S Ribosomal Protein L25
D0: 50S Ribosomal Protein L27
D1: 50S Ribosomal Protein L28
D2: 50S Ribosomal Protein L29
D3: 50S Ribosomal Protein L30
D4: 50S Ribosomal Protein L31
D5: 50S Ribosomal Protein L32
D6: 50S Ribosomal Protein L33
D7: 50S Ribosomal Protein L34
D8: 50S Ribosomal Protein L35
D9: 50S Ribosomal Protein L36
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,222,9471038
Polymers2,198,74354
Non-polymers24,204984
Water57632
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)209.960, 448.860, 624.200
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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RNA chain , 5 types, 10 molecules AACAAVCVAXCXBADABBDB

#1: RNA chain 16S Ribosomal RNA


Mass: 493958.281 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: GenBank: AP008226.1
#23: RNA chain P-site fMet-tRNA


Mass: 24802.785 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: GenBank: AP012306.1
#24: RNA chain mRNA


Mass: 5232.232 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#25: RNA chain 23S Ribosomal RNA


Mass: 947895.375 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: GenBank: AP008226.1
#26: RNA chain 5S Ribosomal RNA


Mass: 39494.535 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: GenBank: AP008226.1

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30S Ribosomal Protein ... , 20 types, 40 molecules ABCBACCCADCDAECEAFCFAGCGAHCHAICIAJCJAKCKALCLAMCMANCNAOCOAPCP...

#2: Protein 30S Ribosomal Protein S2


Mass: 29317.703 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80371
#3: Protein 30S Ribosomal Protein S3


Mass: 26751.076 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80372
#4: Protein 30S Ribosomal Protein S4


Mass: 24373.447 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80373
#5: Protein 30S Ribosomal Protein S5


Mass: 17583.416 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ5
#6: Protein 30S Ribosomal Protein S6


Mass: 11988.753 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SLP8
#7: Protein 30S Ribosomal Protein S7


Mass: 18050.973 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P17291
#8: Protein 30S Ribosomal Protein S8


Mass: 15868.570 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ2, UniProt: A0A0M9AFS9*PLUS
#9: Protein 30S Ribosomal Protein S9


Mass: 14410.614 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80374
#10: Protein 30S Ribosomal Protein S10


Mass: 11954.968 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHN7
#11: Protein 30S Ribosomal Protein S11


Mass: 13737.868 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80376
#12: Protein 30S Ribosomal Protein S12


Mass: 14637.384 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHN3
#13: Protein 30S Ribosomal Protein S13


Mass: 14338.861 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80377
#14: Protein 30S Ribosomal Protein S14


Mass: 7158.725 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ1, UniProt: A0A0N0BLP2*PLUS
#15: Protein 30S Ribosomal Protein S15


Mass: 10578.407 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SJ76
#16: Protein 30S Ribosomal Protein S16


Mass: 10409.983 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SJH3
#17: Protein 30S Ribosomal Protein S17


Mass: 12325.655 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP7, UniProt: A0A0N0BLS5*PLUS
#18: Protein 30S Ribosomal Protein S18


Mass: 10258.299 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SLQ0
#19: Protein 30S Ribosomal Protein S19


Mass: 10605.464 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP2
#20: Protein 30S Ribosomal Protein S20


Mass: 11736.143 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80380
#21: Protein/peptide 30S Ribosomal Protein THX


Mass: 3350.030 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SIH3

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Protein , 1 types, 1 molecules AY

#22: Protein YAEJ


Mass: 15656.146 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: N-terminal His6 / Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: yaeJ / Plasmid: pET28a-YAEJ / Production host: Escherichia coli (E. coli) / Strain (production host): BL21*DE3 / References: UniProt: P40711

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50S Ribosomal Protein ... , 29 types, 58 molecules BDDDBEDEBFDFBGDGBHDHBIDIBNDNBODOBPDPBQDQBRDRBSDSBTDTBUDUBVDV...

#27: Protein 50S Ribosomal Protein L2


Mass: 30532.551 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60405
#28: Protein 50S Ribosomal Protein L3


Mass: 22450.213 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHN8
#29: Protein 50S Ribosomal Protein L4


Mass: 23271.914 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHN9
#30: Protein 50S Ribosomal Protein L5


Mass: 21061.596 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ0
#31: Protein 50S Ribosomal Protein L6


Mass: 19568.926 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ3, UniProt: P0DOY8*PLUS
#32: Protein 50S Ribosomal Protein L9


Mass: 16422.180 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SLQ1
#33: Protein 50S Ribosomal Protein L13


Mass: 15927.903 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60488
#34: Protein 50S Ribosomal Protein L14


Mass: 13323.612 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP8
#35: Protein 50S Ribosomal Protein L15


Mass: 16319.142 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ7
#36: Protein 50S Ribosomal Protein L16


Mass: 15993.909 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60489
#37: Protein 50S Ribosomal Protein L17


Mass: 13750.148 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q9Z9H5
#38: Protein 50S Ribosomal Protein L18


Mass: 12639.845 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ4
#39: Protein 50S Ribosomal Protein L19


Mass: 17188.830 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60490
#40: Protein 50S Ribosomal Protein L20


Mass: 13779.275 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60491
#41: Protein 50S Ribosomal Protein L21


Mass: 11069.153 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60492
#42: Protein 50S Ribosomal Protein L22


Mass: 12808.055 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP3
#43: Protein 50S Ribosomal Protein L23


Mass: 10759.808 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP0
#44: Protein 50S Ribosomal Protein L24


Mass: 12085.611 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP9
#45: Protein 50S Ribosomal Protein L25


Mass: 23238.572 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHZ1
#46: Protein 50S Ribosomal Protein L27


Mass: 9529.074 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60493
#47: Protein 50S Ribosomal Protein L28


Mass: 11004.160 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P60494
#48: Protein 50S Ribosomal Protein L29


Mass: 8670.258 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHP6
#49: Protein 50S Ribosomal Protein L30


Mass: 6799.126 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHQ6
#50: Protein 50S Ribosomal Protein L31


Mass: 8300.543 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SJE1
#51: Protein 50S Ribosomal Protein L32


Mass: 6722.050 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80339
#52: Protein 50S Ribosomal Protein L33


Mass: 6632.896 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P35871
#53: Protein/peptide 50S Ribosomal Protein L34


Mass: 6132.449 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: P80340
#54: Protein 50S Ribosomal Protein L35


Mass: 7506.178 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SKU1
#55: Protein/peptide 50S Ribosomal Protein L36


Mass: 4435.411 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB8 / References: UniProt: Q5SHR2

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Non-polymers , 3 types, 5868 molecules

#56: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2336 / Source method: obtained synthetically / Formula: Mg
#57: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: Zn
#58: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 3518 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.33 Å3/Da / Density % sol: 62.69 %
Crystal growTemperature: 292 K / Method: vapor diffusion / pH: 7.6
Details: 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, vapor diffusion, temperature 292K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.075 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 14, 2011
RadiationProtocol: SINGLE WAVELENGTH / Scattering type: x-ray
Radiation wavelengthWavelength: 1.075 Å / Relative weight: 1
ReflectionResolution: 3.2→35 Å / Num. obs: 956933 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 4.7 % / Rmerge(I) obs: 0.285 / Net I/σ(I): 6.51
Reflection shell
Resolution (Å)Rmerge(I) obsMean I/σ(I) obsDiffraction-ID% possible all
3.2-3.280.0161.06199.8
3.28-3.370.0161.31199.9
3.37-3.470.0161.55199.9
3.47-3.580.0161.87199.9
3.58-3.70.0162.33199.9
3.7-3.820.0162.88199.9
3.82-3.970.0163.58199.9
3.97-4.130.0164.16199.9
4.13-4.310.0164.96199.9
4.31-4.530.0165.85199.9
4.53-4.770.0166.83199.9
4.77-5.060.0167.79199.9
5.06-5.410.0169.13199.9
5.41-5.840.0169.96199.9
5.84-6.40.01610.43199.8
6.4-7.160.01612.48199.8
7.16-8.260.01616.65199.6
8.26-10.120.01622.01199.5
10.12-14.310.01632.52199.2
14.31-350.01641.8190.4

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassificationNB
XSCALEdata scaling
PHENIX1.7.3_928refinement
PDB_EXTRACT3.1data extraction
CBASSdata collection
PHENIXmodel building
XDSdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.2→34.907 Å / Occupancy max: 1 / Occupancy min: 0.2 / SU ML: 0.46 / σ(F): 1.34 / Phase error: 23.88 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2449 47994 5.02 %
Rwork0.1881 --
obs0.191 956625 99.82 %
Solvent computationShrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 38.208 Å2 / ksol: 0.256 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1--7.1642 Å20 Å20 Å2
2--1.5409 Å20 Å2
3---5.6233 Å2
Refinement stepCycle: LAST / Resolution: 3.2→34.907 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms18636 33288 405 449 52778
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.013307681
X-RAY DIFFRACTIONf_angle_d1.574458241
X-RAY DIFFRACTIONf_dihedral_angle_d18.06140205
X-RAY DIFFRACTIONf_chiral_restr0.08758000
X-RAY DIFFRACTIONf_plane_restr0.00924745
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.2-3.23630.366815720.324430107X-RAY DIFFRACTION100
3.2363-3.27440.352215740.307630046X-RAY DIFFRACTION100
3.2744-3.31430.350715780.294630054X-RAY DIFFRACTION100
3.3143-3.35620.334615540.281330206X-RAY DIFFRACTION100
3.3562-3.40030.311815350.269130188X-RAY DIFFRACTION100
3.4003-3.44690.318116360.261630175X-RAY DIFFRACTION100
3.4469-3.49610.315315820.256830131X-RAY DIFFRACTION100
3.4961-3.54820.291916390.244130116X-RAY DIFFRACTION100
3.5482-3.60360.301315520.236230190X-RAY DIFFRACTION100
3.6036-3.66260.28916030.227330138X-RAY DIFFRACTION100
3.6626-3.72570.277815730.216130251X-RAY DIFFRACTION100
3.7257-3.79340.28316270.210830163X-RAY DIFFRACTION100
3.7934-3.86620.265915550.202530221X-RAY DIFFRACTION100
3.8662-3.94510.257915570.191130253X-RAY DIFFRACTION100
3.9451-4.03070.235415940.178930222X-RAY DIFFRACTION100
4.0307-4.12430.239816290.177630239X-RAY DIFFRACTION100
4.1243-4.22730.232316180.168930146X-RAY DIFFRACTION100
4.2273-4.34140.224215860.166630279X-RAY DIFFRACTION100
4.3414-4.46890.223516180.162630282X-RAY DIFFRACTION100
4.4689-4.61280.221816230.156530223X-RAY DIFFRACTION100
4.6128-4.77730.207816020.152630388X-RAY DIFFRACTION100
4.7773-4.96810.223516050.153430254X-RAY DIFFRACTION100
4.9681-5.19350.211515910.149530359X-RAY DIFFRACTION100
5.1935-5.46630.213316450.145930337X-RAY DIFFRACTION100
5.4663-5.80730.203616060.140830442X-RAY DIFFRACTION100
5.8073-6.25340.208916660.14630399X-RAY DIFFRACTION100
6.2534-6.87830.207915760.148530527X-RAY DIFFRACTION100
6.8783-7.86370.207316420.151130565X-RAY DIFFRACTION100
7.8637-9.87020.214516440.16530695X-RAY DIFFRACTION100
9.8702-34.9090.236816120.206431035X-RAY DIFFRACTION98

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