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- EMDB-6943: Saccharomyces cerevisiae Origin Recognition Complex -

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Basic information

Database: EMDB / ID: 6943
TitleSaccharomyces cerevisiae Origin Recognition Complex
Map data
SampleSaccharomyces cerevisiae Origin Recognition Complex:
SourceSaccharomyces cerevisiae (baker's yeast)
Methodsingle particle reconstruction / cryo EM / 8.2 Å resolution
AuthorsLi N / Lam WH / Zhai Y / Cheng J / Cheng E / Zhao Y / Gao N / Tye BK
CitationJournal: Nature / Year: 2018
Title: Structure of the origin recognition complex bound to DNA replication origin.
Authors: Ningning Li / Wai Hei Lam / Yuanliang Zhai / Jiaxuan Cheng / Erchao Cheng / Yongqian Zhao / Ning Gao / Bik-Kwoon Tye
DateDeposition: Apr 22, 2018 / Header (metadata) release: Jul 11, 2018 / Map release: Jul 11, 2018 / Last update: Jul 25, 2018

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.1
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
Supplemental images

Downloads & links


Fileemd_6943.map.gz (map file in CCP4 format, 2917 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
90 pix
2.64 Å/pix.
= 237.6 Å
90 pix
2.64 Å/pix.
= 237.6 Å
90 pix
2.64 Å/pix.
= 237.6 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.64 Å
Contour Level:0.08 (by author), 0.1 (movie #1):
Minimum - Maximum-0.113323666 - 0.3217569
Average (Standard dev.)0.0038152444 (0.019943368)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 237.6 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.642.642.64
M x/y/z909090
origin x/y/z0.0000.0000.000
length x/y/z237.600237.600237.600
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
D min/max/mean-0.1130.3220.004

Supplemental data

Sample components

Entire Saccharomyces cerevisiae Origin Recognition Complex

EntireName: Saccharomyces cerevisiae Origin Recognition Complex / Number of components: 1

Component #1: protein, Saccharomyces cerevisiae Origin Recognition Complex

ProteinName: Saccharomyces cerevisiae Origin Recognition Complex / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

Experimental details

Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionpH: 7.6
VitrificationCryogen name: ETHANE

Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 5 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 45000
3D reconstructionResolution: 8.2 Å / Resolution method: FSC 0.143 CUT-OFF

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