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TitleStructure of the origin recognition complex bound to DNA replication origin.
Journal, issue, pagesNature, Vol. 559, Issue 7713, Page 217-222, Year 2018
Publish dateJul 4, 2018
AuthorsNingning Li / Wai Hei Lam / Yuanliang Zhai / Jiaxuan Cheng / Erchao Cheng / Yongqian Zhao / Ning Gao / Bik-Kwoon Tye /
PubMed AbstractThe six-subunit origin recognition complex (ORC) binds to DNA to mark the site for the initiation of replication in eukaryotes. Here we report a 3 Å cryo-electron microscopy structure of the ...The six-subunit origin recognition complex (ORC) binds to DNA to mark the site for the initiation of replication in eukaryotes. Here we report a 3 Å cryo-electron microscopy structure of the Saccharomyces cerevisiae ORC bound to a 72-base-pair origin DNA sequence that contains the ARS consensus sequence (ACS) and the B1 element. The ORC encircles DNA through extensive interactions with both phosphate backbone and bases, and bends DNA at the ACS and B1 sites. Specific recognition of thymine residues in the ACS is carried out by a conserved basic amino acid motif of Orc1 in the minor groove, and by a species-specific helical insertion motif of Orc4 in the major groove. Moreover, similar insertions into major and minor grooves are also embedded in the B1 site by basic patch motifs from Orc2 and Orc5, respectively, to contact bases and to bend DNA. This work pinpoints a conserved role of ORC in modulating DNA structure to facilitate origin selection and helicase loading in eukaryotes.
External linksNature / PubMed:29973722
MethodsEM (single particle)
Resolution3.0 - 8.2 Å
Structure data

6941

5zr1

EMDB-6941, PDB-5zr1:
Saccharomyces Cerevisiae Origin Recognition Complex Bound to a 72-bp Origin DNA containing ACS and B1 element
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-6942:
Saccharomyces Cerevisiae Origin Recognition Complex Bound to a 36-bp Origin DNA containing ACS and B1 element
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-6943:
Saccharomyces cerevisiae Origin Recognition Complex
Method: EM (single particle) / Resolution: 8.2 Å

Chemicals

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsDNA BINDING PROTEIN/DNA / Origin Recognition Complex / DNA replication initiation / 72-bp origin DNA / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex

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