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- EMDB-6715: FliF ring of Salmonella flagellar motor -

Open data

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Basic information

Database: EMDB / ID: 6715
TitleFliF ring of Salmonella flagellar motor
Map dataFliF ring assembly
SampleFliF ring:
Function / homologySecretory protein YscJ/FliF / Flagellar M-ring protein FliF / Flagellar M-ring C-terminal / Secretory protein of YscJ/FliF family / Flagellar M-ring protein C-terminal / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum-dependent cell motility / motor activity / integral component of membrane / plasma membrane / Flagellar M-ring protein
Function and homology information
SourceSalmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Methodsingle particle reconstruction / cryo EM / negative staining / 22 Å resolution
AuthorsSuzuki H / Yonekura K / Namba K
CitationJournal: J. Mol. Biol. / Year: 2004
Title: Structure of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy and single-particle image analysis.
Authors: Hirofumi Suzuki / Koji Yonekura / Keiichi Namba
DateDeposition: Mar 16, 2017 / Header (metadata) release: Apr 12, 2017 / Map release: Apr 12, 2017 / Last update: Apr 12, 2017

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 1
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 1
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
Supplemental images

Downloads & links


Fileemd_6715.map.gz (map file in CCP4 format, 865 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
60 pix
4.8 Å/pix.
= 288. Å
60 pix
4.8 Å/pix.
= 288. Å
60 pix
4.8 Å/pix.
= 288. Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 4.8 Å
Contour Level:1 (by author), 1 (movie #1):
Minimum - Maximum-0.36197108 - 1.720217
Average (Standard dev.)0.18555644 (0.41004357)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 288 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.84.84.8
M x/y/z606060
origin x/y/z0.0000.0000.000
length x/y/z288.000288.000288.000
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS-30-30-30
D min/max/mean-0.3621.7200.186

Supplemental data

Sample components

Entire FliF ring

EntireName: FliF ring / Details: FliF ring oligomer from Salmonella / Number of components: 1
MassTheoretical: 1.6 MDa

Component #1: protein, FliF ring

ProteinName: FliF ring / Details: FliF ring oligomer from Salmonella / Recombinant expression: No
MassTheoretical: 1.6 MDa
SourceSpecies: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Source (engineered)Expression System: Escherichia coli (E. coli) / Strain: BL21

Experimental details

Sample preparation

SpecimenSpecimen state: particle / Method: negative staining, cryo EM
Sample solutionSpecimen conc.: 5 mg/ml / pH: 8
Staining8% (w/v) ammonium molybdate, 2% (w/v) trehalose
VitrificationCryogen name: ETHANE

Electron microscopy imaging

ImagingMicroscope: HITACHI EF2000
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 2 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 62500 X (calibrated) / Imaging mode: BRIGHT FIELD / Defocus: 0.8 - 3 nm / Energy filter: Hitachi gamma-type energy filter
Specimen HolderModel: OTHER / Temperature: K ( 93 - 93 K)

Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C26 (26 fold cyclic) / Number of projections: 4800
3D reconstructionSoftware: EMAN / Resolution: 22 Å / Resolution method: FSC 0.5 CUT-OFF

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