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- EMDB-6716: Ring assembly of FliF-FliG fusion protein -

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Basic information

Entry
Database: EMDB / ID: 6716
TitleRing assembly of FliF-FliG fusion protein
Map dataRing assembly of FliF-FliG fusion protein
SampleFliFG fusion ring:
Function / homologyFlagellar M-ring C-terminal / FliG C-terminal domain / FliG N-terminal domain / FliG middle domain / Flagellar M-ring protein FliF / Flagellar motor switch protein FliG / Secretory protein YscJ/FliF / Flagellar motor switch protein FliG, alpha-helical / Flagellar M-ring protein C-terminal / Flagellar motor switch protein FliG, C-terminal ...Flagellar M-ring C-terminal / FliG C-terminal domain / FliG N-terminal domain / FliG middle domain / Flagellar M-ring protein FliF / Flagellar motor switch protein FliG / Secretory protein YscJ/FliF / Flagellar motor switch protein FliG, alpha-helical / Flagellar M-ring protein C-terminal / Flagellar motor switch protein FliG, C-terminal / Flagellar motor switch protein FliG, N-terminal domain / Flagellar motor switch protein FliG, middle domain / Secretory protein of YscJ/FliF family / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent cell motility / motor activity / chemotaxis / integral component of membrane / plasma membrane / Flagellar motor switch protein FliG / Flagellar M-ring protein
Function and homology information
SourceSalmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Methodsingle particle reconstruction / cryo EM / negative staining / 25 Å resolution
AuthorsSuzuki H / Yonekura K / Namba K
CitationJournal: J. Mol. Biol. / Year: 2004
Title: Structure of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy and single-particle image analysis.
Authors: Hirofumi Suzuki / Koji Yonekura / Keiichi Namba
DateDeposition: Mar 16, 2017 / Header (metadata) release: Apr 12, 2017 / Map release: Apr 12, 2017 / Last update: Apr 12, 2017

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 1
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 1
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_6716.map.gz (map file in CCP4 format, 865 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
60 pix
4.8 Å/pix.
= 288. Å
60 pix
4.8 Å/pix.
= 288. Å
60 pix
4.8 Å/pix.
= 288. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 4.8 Å
Density
Contour Level:1 (by author), 1 (movie #1):
Minimum - Maximum-0.91259074 - 2.0935736
Average (Standard dev.)0.35862404 (0.46696302)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions606060
Origin-30-30-30
Limit292929
Spacing606060
CellA=B=C: 288 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.84.84.8
M x/y/z606060
origin x/y/z0.0000.0000.000
length x/y/z288.000288.000288.000
α/β/γ90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS-30-30-30
NC/NR/NS606060
D min/max/mean-0.9132.0940.359

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Supplemental data

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Sample components

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Entire FliFG fusion ring

EntireName: FliFG fusion ring / Details: FliF-FliG fusion ring oligomer from Salmonella / Number of components: 1
MassTheoretical: 2.5 MDa

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Component #1: protein, FliFG fusion ring

ProteinName: FliFG fusion ring / Details: FliF-FliG fusion ring oligomer from Salmonella / Recombinant expression: No
MassTheoretical: 2.5 MDa
SourceSpecies: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Source (engineered)Expression System: Escherichia coli (E. coli) / Strain: BL21

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Experimental details

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Sample preparation

SpecimenSpecimen state: particle / Method: negative staining, cryo EM
Sample solutionSpecimen conc.: 5 mg/ml / pH: 8
Staining8% (w/v) ammonium molybdate, 2% (w/v) trehalose
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

ImagingMicroscope: HITACHI EF2000
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 2 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 62500 X (calibrated) / Imaging mode: BRIGHT FIELD / Defocus: 0.8 - 3 nm / Energy filter: Hitachi gamma-type energy filter
Specimen HolderModel: OTHER / Temperature: K ( 93 - 93 K)
CameraDetector: GATAN MULTISCAN

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 3400
3D reconstructionSoftware: EMAN / Resolution: 25 Å / Resolution method: FSC 0.5 CUT-OFF

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