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- EMDB-64694: Cryo- EM structure of ribosomal large subunit (LSU) from Entamoeb... -

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Basic information

Entry
Database: EMDB / ID: EMD-64694
TitleCryo- EM structure of ribosomal large subunit (LSU) from Entamoeba histolytica at 2.8 angstrom resolution
Map data
Sample
  • Complex: Entamoeba histolytica Ribosome Large Subunit
    • RNA: x 3 types
    • Protein or peptide: x 41 types
KeywordsLSU / Entamoeba histolytica / 53S / Ribosome
Function / homology
Function and homology information


protein-RNA complex assembly / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation ...protein-RNA complex assembly / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / endoplasmic reticulum / RNA binding / zinc ion binding
Similarity search - Function
Ribosomal protein L30e / : / Ribosomal protein L2, archaeal-type / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily ...Ribosomal protein L30e / : / Ribosomal protein L2, archaeal-type / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L10e / Ribosomal protein L44e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L44e / Ribosomal protein L44 / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L30e signature 1. / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / : / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal Protein L6, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / : / 60S ribosomal protein L19 / Ribosomal protein L6e / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / Ribosomal protein L30/YlxQ / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L19e, C-terminal domain / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L36e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L37ae / Ribosomal protein L14e domain / Ribosomal L37ae protein family / Ribosomal protein L14 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L14 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal protein L31e / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal_L15e / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal L15 / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L32e / Ribosomal protein L37ae/L37e
Similarity search - Domain/homology
Large ribosomal subunit protein uL4 / 60S ribosomal protein L35a, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L24, putative / 60S ribosomal protein L26, putative / 60S ribosomal protein L32, putative / Ribosomal protein L10, putative / Ribosomal protein L39, putative / 60S ribosomal protein L31, putative / 60S ribosomal protein L5, putative ...Large ribosomal subunit protein uL4 / 60S ribosomal protein L35a, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L24, putative / 60S ribosomal protein L26, putative / 60S ribosomal protein L32, putative / Ribosomal protein L10, putative / Ribosomal protein L39, putative / 60S ribosomal protein L31, putative / 60S ribosomal protein L5, putative / 60S ribosomal protein L29 / 60S ribosomal protein L40, putative / Ribosomal protein L23A, putative / 60S ribosomal protein L21, putative / 60S ribosomal protein L23, putative / 60S ribosomal protein L7a / 60S ribosomal protein L9, putative / 60S ribosomal protein L3, putative / Large ribosomal subunit protein eL22 / Ribosomal protein L19 / 60S ribosomal protein L17, putative / 60S ribosomal protein L18, putative / 60S ribosomal protein L18a / 60S ribosomal protein L34, putative / Large ribosomal subunit protein eL6 / 60S ribosomal protein L11, putative / 60S ribosomal protein L36, putative / 60S ribosomal protein L37-A, putative / 60S ribosomal protein L13, putative / 60S ribosomal protein L38, putative / 60S ribosomal protein L27, putative / 60S ribosomal protein L7, putative / Ribosomal protein L15 / 60S ribosomal protein L14, putative / 60S ribosomal protein L13, putative / 60S ribosomal protein L30, putative / 60S ribosomal protein L37A, putative / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL15A
Similarity search - Component
Biological speciesEntamoeba histolytica HM-1:IMSS (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsSharma S / Mishra S / Gourinath S / Kaushal PS
Funding support India, 1 items
OrganizationGrant numberCountry
Department of Biotechnology (DBT, India)BT/PR45101/DRUG/134/121/2022 India
CitationJournal: Nat Commun / Year: 2025
Title: Cryo-EM structure of ribosome from pathogenic protozoa Entamoeba histolytica reveals unique features of its architecture.
Authors: Shivani Sharma / Shalini Mishra / Samudrala Gourinath / Prem S Kaushal /
Abstract: Entamoeba histolytica, an anaerobic protozoan parasite, is the causative agent of amoebiasis, bloody diarrhea, and liver abscesses in humans. Amoebiasis is more predominant in tropical areas with ...Entamoeba histolytica, an anaerobic protozoan parasite, is the causative agent of amoebiasis, bloody diarrhea, and liver abscesses in humans. Amoebiasis is more predominant in tropical areas with poor sanitation conditions, and it remains the fourth leading cause of death due to a protozoan infection. E. histolytica life cycle spans between an infective 'cyst stage' and an active disease-causing 'trophozoite stage'. We have isolated ribosomes from the trophozoite stage of E. histolytica. Here, we report single particle cryo-EM structures of the 53S ribosome large subunit (LSU), and 75S associated ribosomes, with P-tRNA, A/P and P/E tRNAs, and with paromomycin antibiotic, at 2.8 Å to 3.4 Å resolution. The E. histolytica possesses a reduced ribosome with a conserved core, and the periphery evolved with species-specific unique features. The most notable features are the presence of the rRNA triple helix near the peptide exit tunnel, the expansion segment H88ES102 near the exit site on LSU, and unique insertions in r-proteins. Furthermore, the 75S ribosome paromomycin complex structure provides the atomic details of its interactions. These structures provide insights into the evolutionary adaptation of the E. histolytica translational machinery and may be explored further for amoebicidal therapeutic intervention.
History
DepositionMay 19, 2025-
Header (metadata) releaseOct 8, 2025-
Map releaseOct 8, 2025-
UpdateOct 8, 2025-
Current statusOct 8, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_64694.png

Downloads & links

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Map

FileDownload / File: emd_64694.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesX (Sec.)Y (Row.)Z (Col.)
1.07 Å/pix.
x 300 pix.
= 321. Å		1.07 Å/pix.
x 300 pix.
= 321. Å		1.07 Å/pix.
x 300 pix.
= 321. Å

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
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Contour LevelBy AUTHOR: 2.0
Minimum - Maximum-6.836739 - 18.211324999999999
Average (Standard dev.)-0.000000000001694 (±1.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 321.00003 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_64694_half_map_1.map
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Half map: #1

Fileemd_64694_half_map_2.map
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Sample components

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Entire : Entamoeba histolytica Ribosome Large Subunit

EntireName: Entamoeba histolytica Ribosome Large Subunit
Components
  • Complex: Entamoeba histolytica Ribosome Large Subunit
    • RNA: 25S rRNA (3068-MER)
    • RNA: 5.8S rRNA (145-MER)
    • RNA: 5S rRNA (117-MER)
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: 60S ribosomal protein L3, putative
    • Protein or peptide: 60S ribosomal protein L4, putative
    • Protein or peptide: 60S ribosomal protein L5, putative
    • Protein or peptide: 60S ribosomal protein L6, putative
    • Protein or peptide: 60S ribosomal protein L7, putative
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: 60S ribosomal protein L9, putative
    • Protein or peptide: Ribosomal protein L10, putative
    • Protein or peptide: 60S ribosomal protein L11, putative
    • Protein or peptide: 60S ribosomal protein L13, putative
    • Protein or peptide: 60S ribosomal protein L13, putative
    • Protein or peptide: 60S ribosomal protein L14, putative
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L17, putative
    • Protein or peptide: 60S ribosomal protein L18, putative
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L21, putative
    • Protein or peptide: Large ribosomal subunit protein eL22
    • Protein or peptide: 60S ribosomal protein L23, putative
    • Protein or peptide: Ribosomal protein L23A, putative
    • Protein or peptide: 60S ribosomal protein L24, putative
    • Protein or peptide: 60S ribosomal protein L26, putative
    • Protein or peptide: 60S ribosomal protein L27, putative
    • Protein or peptide: Large ribosomal subunit protein uL15A
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30, putative
    • Protein or peptide: 60S ribosomal protein L31, putative
    • Protein or peptide: 60S ribosomal protein L32, putative
    • Protein or peptide: 60S ribosomal protein L34, putative
    • Protein or peptide: uL29
    • Protein or peptide: 60S ribosomal protein L35a, putative
    • Protein or peptide: 60S ribosomal protein L36, putative
    • Protein or peptide: 60S ribosomal protein L37-A, putative
    • Protein or peptide: 60S ribosomal protein L37A, putative
    • Protein or peptide: 60S ribosomal protein L38, putative
    • Protein or peptide: Ribosomal protein L39, putative
    • Protein or peptide: 60S ribosomal protein L40, putative
    • Protein or peptide: 60S ribosomal protein L44, putative
    • Protein or peptide: Unknown peptide

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Supramolecule #1: Entamoeba histolytica Ribosome Large Subunit

SupramoleculeName: Entamoeba histolytica Ribosome Large Subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote) / Organelle: ribosome / Location in cell: cytoplasm

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Macromolecule #1: 25S rRNA (3068-MER)

MacromoleculeName: 25S rRNA (3068-MER) / type: rna / ID: 1 / Details: 25S rRNA / Number of copies: 1
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 1.130459875 MDa
SequenceString: AGAUUUGAGU AUCGUAAGAG GACUCCCUUA ACUUAAGCAU AUCAAUAAGG GAAGGAAGAA AAAAUAACAA UGAUUUCUUA AGUAAUGGC GAAUGAAAAA GAAAAAGACC AAGUUGAUAA GAAUGGAUAA UUGUAAAAGG GUUAGAAGAA UACAGCAAUG U AUGAGGCU ...String:
AGAUUUGAGU AUCGUAAGAG GACUCCCUUA ACUUAAGCAU AUCAAUAAGG GAAGGAAGAA AAAAUAACAA UGAUUUCUUA AGUAAUGGC GAAUGAAAAA GAAAAAGACC AAGUUGAUAA GAAUGGAUAA UUGUAAAAGG GUUAGAAGAA UACAGCAAUG U AUGAGGCU AGUCUUAAAU UAUUCAGAGU GUAACUAAUA GAAAAGCUAU AUUUAAAAGG GAUAAUUUAA AUUGAAUUCA GA ACGAAUA UUGAGCCAGA GAGAGUGAAA GCCUCGUCAA UUUAGAUAUA UCUUGAAAAG AAUAUGGUUU AGAAAAGAGU AAA UUUAUU UGGAAGUAUA AAUCGAAAAG GUGAUAGUAU UCAUCAAAGU CUAAAUAUUA CAGGAAAACU GAUAGCAAAG AAGU ACUGU GAAGGAAAAC UUAAAAGAAC UUUGGAAAAA GAGUGAAAAG AGCUUGAAAG AGUAAUAUGU GAACGGAUAA UUUAU AAGA AAAAUAGUGG AAUAUGUUAU AAAUAAAGAU GUGAAAAGAU UAGAUAUAUA AGUAGUAUAU UUAAUAAGAU UAUGUU GAA AUUUAUAAUA UAAGAAAUUA UUAAGAGCAA GAGAAUUAUU UCAUUUAUAG UGAUUAUUAA UAAACGAGGA UAUUAGG AG AAAUCUUAGU AUUCAAUUGU GAAUUAAAAG UAAAAAUCCG AUAAUAAUGG AAUGAUAAAA GCUAUUUAAG GUAAGAUU A AUAAAUUUAA UCCCGAGAAU AGAAACUCUU GAAGAAGUAA AUUAUCUACC CGUCUUGAAA CACGGACCAA GGAGUCUAA CAUGGAUGCG AAUGAGAAUA GCAGGAAAGC UAUAACAUGA AGAGAAAACG AAAAACUAUG AGAGUGUAGA AAUACAUUUA GCAAUAGUA UCUUUCAAUA GAAGAAGUAA AUAUGUUAGG ACCCGAAAGA UGGUGAACUA UACUUGAACG GAUUGAAGCC G AAGGAAAC UUUGGUGGAA GAUUCACGCA GUGUUAACGU GCAAAUUACU UGUCAUAUUU GAGUAUAGGG GCGAAAGACU CA UCGAACU AUCUAGUAGC UGGUUCCACC UGAAUAUUCC CUUAGGAAGG UCAAAGUAUU AAUAGUAUUG AUUGGUAAAG AUA AUGAUU AGGUGUAAUG GGUGAGUAUA UUCACUCAAC AUCUUGUCAA ACUAUGAAUA AACAAAGAAG AAUUCUCAGU GUUA AAUAC ACUUCAGGAA UUUAAAUAUA AACAUACUUU GAAUCGGCCA AGUCUGGUAA GCAGGACUGG CGAUGCGGGA UGAAC CGAA CGUCGAGAUA AAGUGCCAAA AAGAAACAUA ACGAGUACCA CAAAAGGUAU UCAUUCAUAU UGACAAUAGG ACGGUG GCC AUGGAAGUCG GAAACCGCUA AGGAAUGUGU AACAACUCAC CUAUCGAAUG AAUGAGUCCU GAAAAUGGAU GGCAUUA UA AUUUCUUAAC UGAUACUCGA CCGUUAAAAU GAAGACAUUU UAACGAGUAA GGAAACGAAA UGGCGUAAGG ACAACAAA A GUAGUAAAGA UUUAAAGUAA UUUAGAUUGA AACUGUCCUU AGUGCAGAUC UUGGUGGUAG UAGCAAAUAC UAAGAAGAG GAAAAAAACA UCUUAGAACU GAAGAGGAGA AAGGUUCCUA AACAACAUUA CUUGGAUUAG GGUGAAUCGA UCCUAAAUUA CAGAUGGAA AUCCUUAAGA AAUAUAUAAG UGACAUAUAG UCAAUAUAAG GAUUGAAAGG GAAUAAGAUU AAUAGUUCUU A AUCAAGAG AAUUAAUGAU UAAAUCUUAG AAAUAAGAUU AAAUCGAAUA AAAUGGUAAC AUAUCAUGAA CUUUAAAAUG GA GUGUGAA AAUCUACGGA AGAGUUCUCU UUUCUUCUUA ACAAAAGUAA AUUCCACUGA AAUGAAUCAA UCAGAGAAGU GGA AUUUAU UGGCUUUGUA AGAAAUUCAA CAUAUGAUUG AAUUGUGGUA UUUUUGCAGU CUUCAUAAAA AUUAAAGGGA AUCU UAGCA UAAAGCUAAU UAAAGAAUUC UUUUGCUCGU ACUAAUAACC GCAACAGGUC UCCAAGAAGC AAAAUCUUUA GUUAA AUAG AAUAACGCAG GUAAGGGAAG UCGGCAACAU AGCUCUGUAA GUUAGCAAGA AAGAGUAGCU CUGGUGAUUU CCUUGU AAA AGGAUUAAAA UCGAAUCAGA ACUUUAAAGG UUAGGGGAAU CCGACUGUUU AAUAAAAACA UAGCAUUCUG AUAAAUC UA AAAGGUUCAU UACAGAAUGU GAUUUCUGCC CAGUGCUCUG AAUGUCAAGG UAAUGAAAUU UACUUAAGCG CGGGUUAA C GGCGGGAGUA ACUAUGACUC UCUUAAGGUA GCCAAAUGCC UCGUCAUCUA AUUAGUGACG CGCAUGAAUG GAUCACCGU GAUUCCCACU GUCCCUAUCU GCAAUCUAGU GAAACCACAG UCAAGGGAAC GGGCUUGAUU GGAACGGCGG GGAAAGAAGA CCCUGUUGA GCUUGACUCU AAUUUAUCAU GUGAAAUCGA UUUUUUGGUA UAGGCUAGGU GGGAGACUUU GUCAAACGUG U AAUACCAC CACUAGAAAU ACUAUUUCAC UCAUUUCGUU AAAUGUUAAA AUGGAUUUUC CAUUAAUUUA AAAACAUUAA AA CUUCGGU UGAAACAGGC GAUUGAAAGA GAUAGACGGG GAGUUUGGCU GGGGCGGCAC AUCUGUUACA AGAUAACGCA GGU GUCCAA AGAUGAACUC AAUGAGGACA GAAACCUCAU GUAGAAUAUA AGGGUAUAAG UUCAUUUGAU UAUUGAUUCC AGUA CAAUC AAUAACUCUG AAAUGAUGGC CUAACGAUCC CUAAGAUUUU UAAUUAGAAA GAAUUUAAAA CAUAUUAGAU UUAUC UGAU GUGUGAGACC GUAAGGUGAU UUCUAGUUAA UCCUUUAAUA AUAAGGCUUG GGGUGUCAGA AAAGUUACCA CAGGGA UAA CUGGCUUGUG GCAGCCAAGC GCUCAUAGCG ACGUUGCUUU UUGAUCCUUC GAUGUCGGCU CUUCCUAUCC AUUUCAA GC AGAAUUGAAA AAAGGUAUGA UUGUUCACCA UUUAAUAGGG AACGUGAGCU GGGUUUAGAC CGUCGUGAGA CAGGUUAG U UUUACCCUAC CGUUUCGGUA GAUUUUCAAA GUAUUUAGAU AGUAGCACAA CUCAGUACGA GAGGAACCGU UGUGUCACG CCACUGGAUU AUUCAAUUAU UUGAUCAAAU AGUGUUGAGA AUCUAACACG UGUUGAAAUA AUGGCUGAAA GCCUCUCAAG CCAGAAUUU CGUCUAGAUG AAUACUUUGU CUUUUUCUUG UUACUUUAGU ACAAUUGAAA AUUAUAUACG UUGUAUUUAU U AUCUCGUG UAUUACUCUA UCAAAAGGGU AGUGAAUACU GAGUUAACAA AAUUUAAAGC AUAACUGUUG CUUAUAGACG AU GGCAUCA GAAAUCGGUG UUGUAAGCUU UAGAGUUUCU UAAUCAAUAC UCUCGGGUAU UGAAUG

GENBANK: GENBANK: BDEQ01000001.1

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Macromolecule #2: 5.8S rRNA (145-MER)

MacromoleculeName: 5.8S rRNA (145-MER) / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 49.892578 KDa
SequenceString:
AAUAAAAUAU UACUUUGGAU AGUUUAGUUU CCUGGGCGAU GAAGAACGCA AUGAAUUGCG AUAAGUGAUA GGAACAAUAA AAUGUGAAU AUCCAAAAUU UGAAUGCUUG AAAGUAUACU UAUGAACUUC AAGGUAUAUA UGAUAUUCAA UAUCCA

GENBANK: GENBANK: X65163.1

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Macromolecule #3: 5S rRNA (117-MER)

MacromoleculeName: 5S rRNA (117-MER) / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 37.450051 KDa
SequenceString:
AGGUAGAUUA CCUGACGUUC CCAUCUGUGU GAACAUAAUG GUACUGAUCA GGCUCUAAGC AUCUUUACUU CCGUGUUCGG AUGGGAACG GGUGUAUCCU ACUUGAUAUG AACCUACC

GENBANK: GENBANK: AY843008.1

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Macromolecule #4: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 27.85002 KDa
SequenceString: MGRRTLSQRK GRGSIFKSRS HHKLGVAQHR AIDFFERHST VRGLVKSIEH DPGRGAPLAR VVFRNPRQYG LDKELFICAE GLYSGQYIY CGSKASLHIG NVLPIGQCPE GTVVCNVESK PGDRGIIARA SGNYATVISH NADNETTRIR LPSGAKKTLK S NCRAMVGI ...String:
MGRRTLSQRK GRGSIFKSRS HHKLGVAQHR AIDFFERHST VRGLVKSIEH DPGRGAPLAR VVFRNPRQYG LDKELFICAE GLYSGQYIY CGSKASLHIG NVLPIGQCPE GTVVCNVESK PGDRGIIARA SGNYATVISH NADNETTRIR LPSGAKKTLK S NCRAMVGI IAGGGRTEKP ILKAGVAYRM YKAKRTTWPR VRGVAMNPVD HPHGGGNHQH VGHPTTLKRS SPPGQKAGKV AA RRTGLIR GGNKEGAADN

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #5: 60S ribosomal protein L3, putative

MacromoleculeName: 60S ribosomal protein L3, putative / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 45.62043 KDa
SequenceString: MSHRKFEAPR HGSLAFHPRK RVHKVRATVS AFPKDNAAEK PHLTGFLGFK AGMTHVIREV KRTNTKLPKD GVLEPVTIIE TPPMVVAGF VGYKKTTTGL KPITAVFAEH IADEFKRRYT KKWYKNTKNQ FAVHTEKYND VKAKSKRERQ IRLIKNRCDV V RVIAHTQM ...String:
MSHRKFEAPR HGSLAFHPRK RVHKVRATVS AFPKDNAAEK PHLTGFLGFK AGMTHVIREV KRTNTKLPKD GVLEPVTIIE TPPMVVAGF VGYKKTTTGL KPITAVFAEH IADEFKRRYT KKWYKNTKNQ FAVHTEKYND VKAKSKRERQ IRLIKNRCDV V RVIAHTQM ALVPLKQKKA EVMEIQINGG SIAEKVDFAV SLLEKQISVN SVFGTDECID VCSVTKGHGY NGVIKRFGVR HL PRKTHRG LRKVACVGAW HPARVSWTVA RAGQMGFFKR TEVNKKIYRL GCGDLKNAKT EFDITEKGIT PMGGFPHYGV VKN DFLMIK GTVAGIRRRV ISLRKACFPS TTRTAQEQIV LKFIDTSSKY GHSRFQTTTE KKARMGPMKK DLERQRAEKV EEGK KL

UniProtKB: 60S ribosomal protein L3, putative

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Macromolecule #6: 60S ribosomal protein L4, putative

MacromoleculeName: 60S ribosomal protein L4, putative / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 47.430117 KDa
SequenceString: MSLRPFVTIY DGITGEAEKT PVRLPAVFLA PIRGDVVHFV YRNQSKNTRQ PEGVSTEAGK QHSAISWGTG RAVARIPRIS GSGSGRNGQ GAFGNMTRKG HMFSPLKNFR KWQRKTPKQM RRFAVASCIA ASAVPALVSA RGHHIAGVPQ IPLVVNSKSI S VIKKTKQA ...String:
MSLRPFVTIY DGITGEAEKT PVRLPAVFLA PIRGDVVHFV YRNQSKNTRQ PEGVSTEAGK QHSAISWGTG RAVARIPRIS GSGSGRNGQ GAFGNMTRKG HMFSPLKNFR KWQRKTPKQM RRFAVASCIA ASAVPALVSA RGHHIAGVPQ IPLVVNSKSI S VIKKTKQA VYLLKKINAY SDVLKVIASK TVRAGQGKMR GRKIKERKGP LVVYGNDDLQ AVKAFRNIPG VDTCRVENLS VL NLAPGCH MGRFIIWTES AFKKLNTIFG TQKKMAQGKS GFRIAHAQMA VPDMKRVVVA AEKAKLLRAK IVLPKVPKRA NPL KNWAAM VKLNPYAKIA SHKLAQVAKA QAAHKAQYAA KMEKILAAKK EALNQSVAKR FGKTQKVDGK VIKVKVENNL LKAT VKRTA KQDAYMKKYD GIVKANAKKY AEKIGF

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #7: 60S ribosomal protein L5, putative

MacromoleculeName: 60S ribosomal protein L5, putative / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 32.437816 KDa
SequenceString: MGFLKVIKNR AYFKKYQTQF RRRREGKTDY YARRKMIFQD KDKFKTPKYR VVVRITNKTV IAQIAYSEII GDKILCAAYS HELPRYGVK LGLTNYAAAY CTGLLLARRL LQKLGMDKQF VGVSDAAKLG EDYTPEEVDE RRPFKCILDV GLARTTTGSK V FAVLKGMC ...String:
MGFLKVIKNR AYFKKYQTQF RRRREGKTDY YARRKMIFQD KDKFKTPKYR VVVRITNKTV IAQIAYSEII GDKILCAAYS HELPRYGVK LGLTNYAAAY CTGLLLARRL LQKLGMDKQF VGVSDAAKLG EDYTPEEVDE RRPFKCILDV GLARTTTGSK V FAVLKGMC DGGVYVPHSA TRFAGAGENE EALRSRILGG HVAAYMKYLS GEDADLYKKQ FGRFIAAGIA PEALENIYIE AH KKIRENP AAEKKEKKEH KKYPTSKKLT LEQRKAAIQA KLAKLAQQA

UniProtKB: 60S ribosomal protein L5, putative

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Macromolecule #8: 60S ribosomal protein L6, putative

MacromoleculeName: 60S ribosomal protein L6, putative / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 22.944295 KDa
SequenceString: MSAPQWFVPV KAGDAKVPAY YPTDDMIQEQ KKKLRTRKGA KITALDKPAW NLEKLRKSIV PGAILIIVAG KYAGKKVVFL KQCIKSGAL IVTGPFKING VPLMRINQRY VIATSQKVDV SKVDTTGVDV KFFKKIAIKK AAFGKTVEED FAKKQYEAKK A LIVEKQKQ ...String:
MSAPQWFVPV KAGDAKVPAY YPTDDMIQEQ KKKLRTRKGA KITALDKPAW NLEKLRKSIV PGAILIIVAG KYAGKKVVFL KQCIKSGAL IVTGPFKING VPLMRINQRY VIATSQKVDV SKVDTTGVDV KFFKKIAIKK AAFGKTVEED FAKKQYEAKK A LIVEKQKQ VDESVVAEIK KVPYLKEYMA SYFTLKNGDH PHLLKF

UniProtKB: Large ribosomal subunit protein eL6

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Macromolecule #9: 60S ribosomal protein L7, putative

MacromoleculeName: 60S ribosomal protein L7, putative / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 25.765818 KDa
SequenceString: MSAKVATAVQ RETCTKTVCP CKARCQAFRK EVILRTIKNH KDIEAAKKAV YTAKKNAEIN GDLYAEADPK LIVAIRIRGI NGVSPKIKK ILKLLRLRQI NNAVFIKANA STIKMLRLVD PYVTYGYPTL ETVQKLIYKR GALKINGNRM PITSNCMIKK A LGDKDVVC ...String:
MSAKVATAVQ RETCTKTVCP CKARCQAFRK EVILRTIKNH KDIEAAKKAV YTAKKNAEIN GDLYAEADPK LIVAIRIRGI NGVSPKIKK ILKLLRLRQI NNAVFIKANA STIKMLRLVD PYVTYGYPTL ETVQKLIYKR GALKINGNRM PITSNCMIKK A LGDKDVVC VDDLVHEIYT VGKHFKEVNN KLAPFKLNGA KIAEKNKKIH FILGGGFGNR ELLINKLLAN MI

UniProtKB: 60S ribosomal protein L7, putative

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Macromolecule #10: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 32.313945 KDa
SequenceString: MSGKSNKQKQ AKQTNPLIVA NKKVFGKGNV AHPRDLTRYV KYPLYVRIQK EKRLLMKRLK TPPAVNIFAN HTLDKTNATQ LFKILDHIK PEERAAKLQR IRAAAKELAE KTQKGEKATA SKKTEKPATL SYGINNVVRL IERKQAKLVV IAHDVEPLEM V VYLPYLCK ...String:
MSGKSNKQKQ AKQTNPLIVA NKKVFGKGNV AHPRDLTRYV KYPLYVRIQK EKRLLMKRLK TPPAVNIFAN HTLDKTNATQ LFKILDHIK PEERAAKLQR IRAAAKELAE KTQKGEKATA SKKTEKPATL SYGINNVVRL IERKQAKLVV IAHDVEPLEM V VYLPYLCK KLQVPYCIVK GKARLGQLIH RSTAAVVAVT EIKKEDKAAF ESLVQNVKSI YFENAHMYRE FGGRINGFKH NE KQKKIQS KLNKDLADKA KQQAALTVTT AVEEKAAPVA EEKKADKTA

UniProtKB: 60S ribosomal protein L7a

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Macromolecule #11: 60S ribosomal protein L9, putative

MacromoleculeName: 60S ribosomal protein L9, putative / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 22.351932 KDa
SequenceString: MPRLLKKTEH VVIPEGVNIE INRKNIKVTG PRGSLTREFQ HPKVDIYASK LVVKKEGPKE NVVVIDMWYG NRRDSAVVRT IASHIKNMI TGVTKGYQYK MRVVYAHFPV TLVIADDGKS IQVQNFLGEK RTRHIEMYDG VVVKKLGKDE ICLEGNDVEK V SQSAANIH ...String:
MPRLLKKTEH VVIPEGVNIE INRKNIKVTG PRGSLTREFQ HPKVDIYASK LVVKKEGPKE NVVVIDMWYG NRRDSAVVRT IASHIKNMI TGVTKGYQYK MRVVYAHFPV TLVIADDGKS IQVQNFLGEK RTRHIEMYDG VVVKKLGKDE ICLEGNDVEK V SQSAANIH AANLVRNKDI RQFLDGVYVS EKCLIENDA

UniProtKB: 60S ribosomal protein L9, putative

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Macromolecule #12: Ribosomal protein L10, putative

MacromoleculeName: Ribosomal protein L10, putative / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 23.831152 KDa
SequenceString: MGRRPGRCYR LVRGHPYPKS KYCRGVPDPR IKLFDIGNRS APCDDFPCCV HIVGLERENI SSEAMEAARI SINKNMLKYA GKDGFHVRI RIHPFHVLRI NKMLSCAGAD RLQTGMRGAW GKSYGSCARV KVGQVLISGR CKEQHLPAMI KSFRLACYKF A GRQKLVIS ...String:
MGRRPGRCYR LVRGHPYPKS KYCRGVPDPR IKLFDIGNRS APCDDFPCCV HIVGLERENI SSEAMEAARI SINKNMLKYA GKDGFHVRI RIHPFHVLRI NKMLSCAGAD RLQTGMRGAW GKSYGSCARV KVGQVLISGR CKEQHLPAMI KSFRLACYKF A GRQKLVIS NKWGFTKYTK EEYQQLKKDG KIIADGCYFK LATTKGPLPK VN

UniProtKB: Ribosomal protein L10, putative

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Macromolecule #13: 60S ribosomal protein L11, putative

MacromoleculeName: 60S ribosomal protein L11, putative / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 19.669807 KDa
SequenceString:
MSACKSDVMR KIKIDKLVLN ISVGESGDRL TRAAKVLETL TGQTPVLSEA RYTVRAFSIR RNDQIACHVT VRGAKAAEIL EKGLKVKEF ELKANNFSAT GNFGFGIEEH IDLGLKYDPQ VGIYGMDFFV VLARPGFRVS RRKRMQDKIG RDHLLTKEDA I NWFKETYE GIVLNK

UniProtKB: 60S ribosomal protein L11, putative

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Macromolecule #14: 60S ribosomal protein L13, putative

MacromoleculeName: 60S ribosomal protein L13, putative / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 33.028422 KDa
SequenceString: MKHNQAVSNI HFCKDWRSKV HTWFKQPFRK IRRHQTRVEK AKAVFPATIK SLKPSVHCMN QRFNYKLRLG RGFTLKELRA AKIDKNLAR TIGIAVDPRR KESSKECLNR NAQRLTEYMN RLVVLPKVHA ATAKRLVLNK KNAEAKTKKA AEIKKFIAEH N KTIKELKI ...String:
MKHNQAVSNI HFCKDWRSKV HTWFKQPFRK IRRHQTRVEK AKAVFPATIK SLKPSVHCMN QRFNYKLRLG RGFTLKELRA AKIDKNLAR TIGIAVDPRR KESSKECLNR NAQRLTEYMN RLVVLPKVHA ATAKRLVLNK KNAEAKTKKA AEIKKFIAEH N KTIKELKI KVAAAKKDYA KTKDAAKAKE LKACLKGLKK AQVAFAAQVA KEQKKTKQFN KLPVQQKEAK QVLDANKVIR LT APCTLET KTLTKGMKAF DAVAHLRKAK NVAKAVSGIV KGQKKANNAP SKKQ

UniProtKB: 60S ribosomal protein L13, putative

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Macromolecule #15: 60S ribosomal protein L13, putative

MacromoleculeName: 60S ribosomal protein L13, putative / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 23.177395 KDa
SequenceString: MSTNNKVTVI DCHGHVLGRV ASVVAKHLLL GQKFVLVRCE DLQVCGTLKM RLVQWELYQR KRVNTNPTRG AFHHRSPADM VRKAVRGML PKNNYRGKLA LKNLKCFEGC PAPYDKMKKS SIPEAAAIYS FNPTRKRTLL GELGTAVGWK YADVVAKNEA E RKERAAKW ...String:
MSTNNKVTVI DCHGHVLGRV ASVVAKHLLL GQKFVLVRCE DLQVCGTLKM RLVQWELYQR KRVNTNPTRG AFHHRSPADM VRKAVRGML PKNNYRGKLA LKNLKCFEGC PAPYDKMKKS SIPEAAAIYS FNPTRKRTLL GELGTAVGWK YADVVAKNEA E RKERAAKW YAAKQLKQKA VAEAKEKILA DEKYKAKVAI LKKFGYA

UniProtKB: 60S ribosomal protein L13, putative

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Macromolecule #16: 60S ribosomal protein L14, putative

MacromoleculeName: 60S ribosomal protein L14, putative / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 15.161096 KDa
SequenceString:
MVFSRFVEVG RVVLMKTGPF ASKLAVIVEI LDHNRVLVDG PQAITGVPRQ VVNLNTISLT SFVVNKVTRG MTHKKLCLRF TQDAIIKRF NKTATGKAIE KRILREKMTD FDKFQVSIIN RKINAAAKIA VSKKAH

UniProtKB: 60S ribosomal protein L14, putative

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Macromolecule #17: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 23.951283 KDa
SequenceString: MGAYKYINAL NRKKQSDVLR FLRRIRCWEL RHLPRIHRVA RPTNPERARR LGYKPKSGFV IYSVRIHRGN RKVSARKGIV HRKPACQGI HKIKRVKSLQ AIAEQRVGKH CGALRVLNSY FIGQDSTYRF FEVILVDPFN AAIRNDPKMN WICADKQKRR E ARGLTSAA ...String:
MGAYKYINAL NRKKQSDVLR FLRRIRCWEL RHLPRIHRVA RPTNPERARR LGYKPKSGFV IYSVRIHRGN RKVSARKGIV HRKPACQGI HKIKRVKSLQ AIAEQRVGKH CGALRVLNSY FIGQDSTYRF FEVILVDPFN AAIRNDPKMN WICADKQKRR E ARGLTSAA KKSRGFTGKG RRYNKIGAGA RGNLKRRNLL QLKRYRS

UniProtKB: Ribosomal protein L15

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Macromolecule #18: 60S ribosomal protein L17, putative

MacromoleculeName: 60S ribosomal protein L17, putative / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 19.89243 KDa
SequenceString:
MVKYCAKPVN VAKAAQARGD NLKVHFKNTY ETADAIRGMK VARAQEFLKN VIIKKEIVPF RKHNGGVGRK AQCKGLNCAF GRWPVKSAQ FILKLLKNAV DNAKAKGLNT ALLTVSHIAV QEARSSRRRL YRAHGSINSF SSSPCHIELI LTEKEKKVAK P TEGIKGKK STHQKIAYVL N

UniProtKB: 60S ribosomal protein L17, putative

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Macromolecule #19: 60S ribosomal protein L18, putative

MacromoleculeName: 60S ribosomal protein L18, putative / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 19.038791 KDa
SequenceString:
MAIDLDRHHV RKHVSKTARG NNAYMKLLVR LYGFLARRTQ SKFAKTILHR LCLSRVNRPI VSTSKLACLM KKHPEETAVC VNTVTYDSR YPVPKMNVCA LKFTKTAEAA ITKAGGKCLR FDELALKAPT GRKTVLIRGK RNVREALKHF GKVCAKKNPA K QYKGKQTK

UniProtKB: 60S ribosomal protein L18, putative

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Macromolecule #20: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 20.130812 KDa
SequenceString:
MKEYYITARC TDKAHNPNEI FRSRVFAPNY VVAKSKFWMM LKKQHKVKKI NAELLQCQPI FEKNPTVVKN YQIFLRYVSA GGIHNITKQ YRDVTRVGAV RQMYNDLASQ YRTRQDKIQL ISITTVGDKA VKNPVLHQFV GHHVKFPMVH CKPVIASKRF N LPIKAGKL TTKLC

UniProtKB: 60S ribosomal protein L18a

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Macromolecule #21: Ribosomal protein L19

MacromoleculeName: Ribosomal protein L19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 22.978787 KDa
SequenceString: MVSLKLQKRL AASVLKCGNR KVWLDPNETP EISLANSRKA IRKLIKDRLI IKKPAAMHSR ARVVERKQAK ILGRHTGHGK RKGTKNART PQKTLWIIRM RVLRRLLLKY RTTGKIDKHM YHDFYLKVKG NQYKNKKALM ESIFKALEEK KLLAKASVQV T KKSTFITE ...String:
MVSLKLQKRL AASVLKCGNR KVWLDPNETP EISLANSRKA IRKLIKDRLI IKKPAAMHSR ARVVERKQAK ILGRHTGHGK RKGTKNART PQKTLWIIRM RVLRRLLLKY RTTGKIDKHM YHDFYLKVKG NQYKNKKALM ESIFKALEEK KLLAKASVQV T KKSTFITE KKPVVAEEPK KVVEKKEEKK SKAGKKNAKK

UniProtKB: Ribosomal protein L19

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Macromolecule #22: 60S ribosomal protein L21, putative

MacromoleculeName: 60S ribosomal protein L21, putative / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 18.869338 KDa
SequenceString:
MPLSNGRNRR TRKLFKRPFR GHGLSNTSTI LHTYKVGDYV TILCNSSIQH GLPYKAFHGK TGRVWNVNPH AIGVMVNKKV NNRIVVKRL HISPEHIKPS GCQKDFLERK AAVAAIRKQN IQLKKEGKPL LPLPAKRLPK QPRPAELIKG ADIKFTTVAP L KFEELY

UniProtKB: 60S ribosomal protein L21, putative

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Macromolecule #23: Large ribosomal subunit protein eL22

MacromoleculeName: Large ribosomal subunit protein eL22 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 15.877571 KDa
SequenceString:
MSKTIKAPAA NKVLVEKKKI NKTEEKTFSC TIDLTNCTDF IEPKKLVTFF RQTIKVQGRA GNTKGIEVKV ADKKVTITTS SAKLCKRYM KYLMKKYLKK NNLREWLRVI SDKKDGFELK FFNVQNDEEE VAEETNAQ

UniProtKB: Large ribosomal subunit protein eL22

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Macromolecule #24: 60S ribosomal protein L23, putative

MacromoleculeName: 60S ribosomal protein L23, putative / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 15.26081 KDa
SequenceString:
MPKRGAGGRQ GNKFRVTCGL NNASTVNCAD NTGAKTLTII SVKGFHGRLN RLPRAGCGDM VVATCKKGKP EYRKKMHTAV IIRQRRTWR RKDGVTLYFE DNAAVIVNMK GEMKGSAITG PVSKESADLW PKISSNAPTI V

UniProtKB: 60S ribosomal protein L23, putative

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Macromolecule #25: Ribosomal protein L23A, putative

MacromoleculeName: Ribosomal protein L23A, putative / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 13.694312 KDa
SequenceString:
MAGTKRYTTA FHRPATKNAA KAPKYPRSLK ANRNKMDEFS IIKFPLATET ALKKIEDHNT LVFLCEQKAN KTMIKKAVEK RYGVKVIKV NTLVRLDGLK KAFVRLAPDV EAMEVATKIG LF

UniProtKB: Ribosomal protein L23A, putative

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Macromolecule #26: 60S ribosomal protein L24, putative

MacromoleculeName: 60S ribosomal protein L24, putative / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 18.649971 KDa
SequenceString:
MSIKTQVCAF SGFKIPVAKG RKYVRLDLKS FTFINKKSLM QFLRKTNPRK VFWTAIYRQL NKKGQQNELV EKKKTRRVIK VNRGYSGIS AEEIAKRKQT QSSEVLAKKR NAAIKEIKEK KAENAAKKEA ARKEAKANGK KATNTYKKSN AKLPKAGHAN F NSR

UniProtKB: 60S ribosomal protein L24, putative

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Macromolecule #27: 60S ribosomal protein L26, putative

MacromoleculeName: 60S ribosomal protein L26, putative / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 23.898668 KDa
SequenceString: MKFNCKVSSN PSKARKALYT ANASQRALMM SARLSKELRE KYNVIAIPIH KDDEVKIIKG HQKVAGKVTA VRRSKYVINI DKLTKTKAN GQTVPIAIRP SNVIITKLFL NKDREALLAK KAESRKSYKE LVAKRNEEVA KAFTAVYPAL NMDIFGKKVE P ITEKKPIR ...String:
MKFNCKVSSN PSKARKALYT ANASQRALMM SARLSKELRE KYNVIAIPIH KDDEVKIIKG HQKVAGKVTA VRRSKYVINI DKLTKTKAN GQTVPIAIRP SNVIITKLFL NKDREALLAK KAESRKSYKE LVAKRNEEVA KAFTAVYPAL NMDIFGKKVE P ITEKKPIR IPSKKMAEKL MSKPTLRAYK TGIKVHKAKN AIAAYKAALA KKIAK

UniProtKB: 60S ribosomal protein L26, putative

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Macromolecule #28: 60S ribosomal protein L27, putative

MacromoleculeName: 60S ribosomal protein L27, putative / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 15.786719 KDa
SequenceString:
MSKILAPGRV VIILSGRFAG KKAVVSSVNL QGTKDRKYGF VTVVGVERAP LKITRKMSAK VQEMRTSVKS FCKVINVNHV MPTKYTVNL EQFNLIKQVK INNFEAGKVY PISQKKAISS QFAEEYRKGK ESWLFTKLRF

UniProtKB: 60S ribosomal protein L27, putative

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Macromolecule #29: Large ribosomal subunit protein uL15A

MacromoleculeName: Large ribosomal subunit protein uL15A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 17.042818 KDa
SequenceString:
MATRFRQTRR RRGHVSMGYG RIGKHRKQRG GRGNAGGQHH RKTWFTTFHP DYFGKHGMRV FHLKANKYYC PSINVDSLWS LVGKDVQAQ YKNAKVGEEV PVIDCVKHGY FKVLGKGFLP KQPVIVRARY FSEKAQQKIK AVGGACELTA

UniProtKB: Large ribosomal subunit protein uL15A

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Macromolecule #30: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 7.340583 KDa
SequenceString:
MSKDKNRSSH NQSHKAHRNG FYKPKKSAYM STKGMNVQVL KNTKAQRKFA LAKKLEAKKA ANKE

UniProtKB: 60S ribosomal protein L29

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Macromolecule #31: 60S ribosomal protein L30, putative

MacromoleculeName: 60S ribosomal protein L30, putative / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 11.629423 KDa
SequenceString:
MSSTKQAKKT QEGVNSKLAL VTKSGKYVLG TKATLKTIRD GKSKLVIISS NCPPLERAKI EYYAVLGKVQ VVHYSGSNVD LGTACGRYF SVSVLSIIDG GDSDILKASE

UniProtKB: 60S ribosomal protein L30, putative

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Macromolecule #32: 60S ribosomal protein L31, putative

MacromoleculeName: 60S ribosomal protein L31, putative / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 17.281588 KDa
SequenceString:
MAVSKTTNPR VLKYREANKA RKEAKKAKAA NKKPAKEIKQ KEMAPITREM TIHMHAYLHK ESFKKRAPKA IKIIRFLAIK TMKTHVVKF DMGLNQFIWS QGIRSVADRI RVRMARLPIE GEEGKFYTLV SYVPVASFKG LVTKTVEEAE N

UniProtKB: 60S ribosomal protein L31, putative

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Macromolecule #33: 60S ribosomal protein L32, putative

MacromoleculeName: 60S ribosomal protein L32, putative / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 15.572594 KDa
SequenceString:
MVGPVIKPKI VKKYTGHFKR FDSNRFLRVK ASWRVPRGID NRLRRKFKGT NLLPKIGYGS AKATRNMLPC GLYKFPVRNT TDLEMIMMQ NRKYAAEISH AVSAKKRQEI IQRAKILNIR VLNADARIKK QEVAQ

UniProtKB: 60S ribosomal protein L32, putative

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Macromolecule #34: 60S ribosomal protein L34, putative

MacromoleculeName: 60S ribosomal protein L34, putative / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 15.481444 KDa
SequenceString:
MAVRVTYVRK HSYNTKSNGI RIVKTAGGNM TVQHRQKISN GVRCGDCGCV LAGIRHIRPH QYGWLGKSKR TVTRAYGGVK CHKCVQKKI LRAFLINEHN LIKLAAAKTN LEKKQSVKED KKKATKTVKK ADKKTQKK

UniProtKB: 60S ribosomal protein L34, putative

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Macromolecule #35: uL29

MacromoleculeName: uL29 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 13.925793 KDa
SequenceString:
ASLKISAIKA MSKNDMNAKL IELKGELANL RTAKVTGGAP SKISRLRVVK KDIARLLTVM NTQRMEALRK YYKQAKYVPK ELRPKTTRK ERRQLTKAQL NLKTVKAAKK AQHFPARKFF VLA

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Macromolecule #36: 60S ribosomal protein L35a, putative

MacromoleculeName: 60S ribosomal protein L35a, putative / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 12.143319 KDa
SequenceString:
MSKCRLHVNA VFMGFQRAQR NQYPNVALLQ ISGVNTKEDT PFYIGKRVAY VYKVNVKKGS NKPAIRAIFG KIVAPHGNHG VVKARFLHN LPPKAMGQNL KVFLYPSNI

UniProtKB: 60S ribosomal protein L35a, putative

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Macromolecule #37: 60S ribosomal protein L36, putative

MacromoleculeName: 60S ribosomal protein L36, putative / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 11.942095 KDa
SequenceString:
MARGIAVGLK RGYPVHTMKT AKRHYGVTKR KHVVNDVIRE ACGFSAYERH MMDLLRRGLD KKALKYAKKH LGTHKRGLAK RDEIQRALE AIKAAHAHLG HHEQH

UniProtKB: 60S ribosomal protein L36, putative

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Macromolecule #38: 60S ribosomal protein L37-A, putative

MacromoleculeName: 60S ribosomal protein L37-A, putative / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 9.046786 KDa
SequenceString:
MTKGTSCRGT RHNKTHILCK RCGKRSWHLQ KQRCASCGYP DAKMRQYAWG YKALRRRTQG TGRMRHLKIA LKRTTKA

UniProtKB: 60S ribosomal protein L37-A, putative

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Macromolecule #39: 60S ribosomal protein L37A, putative

MacromoleculeName: 60S ribosomal protein L37A, putative / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 10.249147 KDa
SequenceString:
MARRTKKVGI VGKYGTRYGA SLRKVIKKIE IAQHSKYVCP FCCKEAVRRT ACGIWECKGC GKQIAGGAYT LSTISGATVR STVRRLRDA KNSQ

UniProtKB: 60S ribosomal protein L37A, putative

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Macromolecule #40: 60S ribosomal protein L38, putative

MacromoleculeName: 60S ribosomal protein L38, putative / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 9.586526 KDa
SequenceString:
MVCFIQLSPK QVQEFKQILK LLKSGSIRAV RVKTNGENTK FKVRCAKYLY TYICADKAKA EELKKAIPKD IHVVEIAKKA PKAE

UniProtKB: 60S ribosomal protein L38, putative

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Macromolecule #41: Ribosomal protein L39, putative

MacromoleculeName: Ribosomal protein L39, putative / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 6.282477 KDa
SequenceString:
MTGHKGFSYK LKLGKKIHQN RGVPQWFRMK TGNTINYNFK RRHWNRRKMG M

UniProtKB: Ribosomal protein L39, putative

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Macromolecule #42: 60S ribosomal protein L40, putative

MacromoleculeName: 60S ribosomal protein L40, putative / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 6.482917 KDa
SequenceString:
MGIEPALEAI ARKYNCDKMI CRRCYARLPL RATTCRKKKC GHSGDLRPKK KIQSKK

UniProtKB: 60S ribosomal protein L40, putative

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Macromolecule #43: 60S ribosomal protein L44, putative

MacromoleculeName: 60S ribosomal protein L44, putative / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 11.603004 KDa
SequenceString:
MVVVPKTRKT YCPRCDKHTP HKVSQYKKGK DSLHVQGKRR YDRKQQGFGG QTKPVFKKKA KTTKKVVLKI ECTKCKYKLQ KCLKRCKHF ELGNEKKDK

UniProtKB: 60S ribosomal protein L44, putative

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Macromolecule #44: Unknown peptide

MacromoleculeName: Unknown peptide / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Entamoeba histolytica HM-1:IMSS (eukaryote)
Molecular weightTheoretical: 1.124378 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 7.4
Details: 20nM HEPES-sodium salt buffer pH 7.4, 100mM Potassium acetate, 10mM Magnesium acetate, 10mm Ammonium acetate, 3mM DTT
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 50 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 290 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Number real images: 3470 / Average exposure time: 2.0 sec. / Average electron dose: 1.34 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 75000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.8 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 763731
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: EMDB MAP
EMDB ID:

2660

Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 378061
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 2
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChain

5xxb

source_name: PDB, initial_model_type: experimental model

4ug0

source_name: PDB, initial_model_type: experimental model
Detailsphenix.real_space_refinement
RefinementSpace: REAL / Protocol: FLEXIBLE FIT / Overall B value: 67.76
Output model

PDB-9v1i:
Cryo- EM structure of ribosomal large subunit (LSU) from Entamoeba histolytica at 2.8 angstrom resolution

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