EMDB-64679: Cryo-EM structure of the 48-nm repeat doublet microtubule from mo...

Basic information

Entry

Database: EMDB / ID: EMD-64679

• Title: Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm

Sample

• Complex: doublet microtubule of mouse sperm flagella
  • Protein or peptide: x 60 types
• Ligand: x 4 types

• Keywords: sperm flagellar doublet microtubules / STRUCTURAL PROTEIN

Function / homology

Function:

male germ-line stem cell population maintenance / protein localization to motile cilium / epithelial cilium movement involved in determination of left/right asymmetry / left/right pattern formation / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / axonemal microtubule doublet inner sheath / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / outer dense fiber / sperm axoneme assembly / protein polyglutamylation / positive regulation of feeding behavior / cerebral cortex cell migration / regulation of calcineurin-NFAT signaling cascade / regulation of microtubule nucleation / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cargo trafficking to the periciliary membrane / Sealing of the nuclear envelope (NE) by ESCRT-III / cerebrospinal fluid circulation / inner dynein arm assembly / epithelial cilium movement involved in extracellular fluid movement / Carboxyterminal post-translational modifications of tubulin / Intraflagellar transport / MAP kinase tyrosine/serine/threonine phosphatase activity / cilium-dependent cell motility / regulation of cilium beat frequency involved in ciliary motility / COPI-independent Golgi-to-ER retrograde traffic / 9+2 motile cilium / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / left/right axis specification / regulation of store-operated calcium entry / intraciliary transport / cilium movement involved in cell motility / COPI-mediated anterograde transport / acrosomal membrane / axoneme assembly / microtubule sliding / Kinesins / cilium movement / sperm DNA condensation / perinuclear theca / PKR-mediated signaling / Aggrephagy / RHO GTPases activate IQGAPs / motile cilium assembly / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / COPI-dependent Golgi-to-ER retrograde traffic / Resolution of Sister Chromatid Cohesion / calcium ion sensor activity / The role of GTSE1 in G2/M progression after G2 checkpoint / Recycling pathway of L1 / cilium organization / axonemal microtubule / cellular response to UV-C / gamma-tubulin ring complex / Hedgehog 'off' state / RHO GTPases Activate Formins / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / cellular response to leukemia inhibitory factor / Separation of Sister Chromatids / Anchoring of the basal body to the plasma membrane / Recruitment of NuMA to mitotic centrosomes / AURKA Activation by TPX2 / manchette / positive regulation of cell motility / ciliary transition zone / determination of left/right symmetry / sperm head-tail coupling apparatus / positive regulation of cilium assembly / Regulation of PLK1 Activity at G2/M Transition / MHC class II antigen presentation / 3'-5'-DNA exonuclease activity / UTP biosynthetic process / CTP biosynthetic process / motile cilium / DNA catabolic process / protein targeting to membrane / nucleoside diphosphate kinase activity / extrinsic component of membrane / : / single fertilization / mRNA stabilization / sperm principal piece / GTP biosynthetic process / intermediate filament / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / spermatid development / AMP binding / centriolar satellite / tubulin complex / protein-serine/threonine phosphatase / intercellular bridge

Domain/homology:

Spermatogenesis-associated protein 48 / Protein of unknown function DUF4555 / Spermatogenesis-associated protein 48 / Sperm-associated microtubule inner protein 4 / Uncharacterized protein C1orf100 / : / Family of unknown function (DUF5530) / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 / Testis-expressed protein 33 / Testis-expressed sequence 37 protein / CFAP97 domain-containing protein 1/2 / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / : / : / Normal lung function maintenance, Low in Lung Cancer 1 protein / Domain of unknown function (DUF4562) / Spermatid-specific manchette-related protein 1 / TSC21 family / Testis-expressed sequence 33 protein family / Sperm-associated microtubule inner protein 5 / Ciliary microtubule inner protein 3 / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Sperm microtubule inner protein 8 / Sperm microtubule inner protein 11 / : / : / Sperm microtubule inner protein 1 / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Cilia- and flagella-associated protein 68 / Sperm-associated microtubule inner protein 10 / Protein of unknown function DUF4541 / Tektin bundle interacting protein 1 / Hemingway/CFA97 / Coiled-coil domain-containing protein 105 / : / Hemingway/CFA97 / Tektin bundle interacting protein 1 / Cilia and flagella associated protein 90 / : / FAM183 family / FAM183A and FAM183B related / Sperm-tail PG-rich repeat / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Sperm-tail PG-rich repeat / Protein CFAP95 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / : / Tektin family / Protein of unknown function (DUF3695) / DJBP, EF-hand domain / EF-hand domain / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 161-like domain / : / : / EFHB C-terminal EF-hand domain / : / : / Nucleoside diphosphate kinase 7 N-terminal PH domain / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / :

Component:

Cilia- and flagella-associated protein 77 / Luteinizing hormone beta / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Protein SPMIP1 / Sperm microtubule inner protein 2 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Stabilizer of axonemal microtubules 4 / Tektin-4 / Sperm microtubule inner protein 6 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 107 / Piercer of microtubule wall 1 protein / Cilia- and flagella-associated protein 52 / Cilia- and flagella-associated protein 144 / Protein SPMIP7 / Meiosis-specific nuclear structural protein 1 / Sperm microtubule inner protein 8 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Cilia- and flagella-associated protein 161 / Enkurin / Tektin-3 / EF-hand calcium-binding domain-containing protein 3 / Cilia- and flagella-associated protein 20 / Sperm microtubule inner protein 11 / EF-hand domain-containing family member B / Ciliary microtubule inner protein 3 / Ciliary microtubule associated protein 1A / Tektin-2 / Testis-specific serine/threonine-protein kinase 6 / Cilia- and flagella-associated protein 95 / Sperm-associated microtubule inner protein 5 / RIB43A-like with coiled-coils protein 1 / Cilia- and flagella-associated protein 68 / Cilia- and flagella-associated protein 53 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 2A / Tektin-like protein 1 / RIB43A-like with coiled-coils protein 2 / Sperm-associated microtubule inner protein 4 / Dual specificity phosphatase 21 / Cilia- and flagella-associated protein 141 / Sperm-associated microtubule inner protein 10 / Ciliary microtubule inner protein 4 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 45 / Ciliary microtubule inner protein 1 / Protein SPMIP3 / Cilia- and flagella-associated protein 276 / Protein SPMIP9 / Tektin-1 / Parkin coregulated gene protein homolog / Sperm axonemal maintenance protein CFAP97D1 / Cilia- and flagella-associated protein 90 / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 2 protein

• Biological species: Mus musculus (house mouse)
• Method: single particle reconstruction / cryo EM / Resolution: 3.7 Å
• Authors: Liu Q / Gui M / Wu JP / Zhou LN

Funding support

China, 1 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)		China

• Citation: Journal: Nat Commun / Year: 2026; Title: Doublet microtubule-associated tektins and enzymes differentially regulate sperm flagellar integrity and motility.; Authors: Qi Liu / Lunni Zhou / Xiaochen Liang / Pengyu Chen / Bo Li / Shuo Yang / Yuqi Liu / Haibo Zhao / Jin Hu / Shan Feng / Shanshan Xie / Jianping Wu / Miao Gui / ; Abstract: Doublet microtubule (DMT)-associated proteins assemble and drive sperm flagella, which are essential for successful fertilization. However, the exact roles of different DMT-associated proteins in sperm function and the underlying molecular mechanisms remain elusive. Here, we generate four gene-knockout mice based on high-resolution structures targeting distinct DMT components: two intermediate filament-like tektins (TEKT1, TEKT5) and two enzymes (TSSK6, DUSP21). The depletion of TEKT1, shared by sperm flagella and motile cilia, causes male infertility characterized by impaired sperm motility and loss of the tektin bundle, whereas sperm-specific Tekt5 knockout (KO) mice remain fertile with largely normal flagellar function, indicating functional divergence within the tektin family. Tssk6 KO spermatozoa exhibit severely disturbed morphology and motility, resulting in homozygote infertility and heterozygote subfertility. Phosphoproteomics reveals dysregulated phosphorylation of axonemal proteins, highlighting the critical role of kinase-mediated signaling in regulating sperm motility. Conversely, Dusp21 KO mice display no fertility or sperm motility defects, suggesting compensatory phosphatase activity. Phenotypic comparisons between Tekt1 and Tssk6 KO mice suggest their involvement in distinct subtypes of asthenozoospermia. Overall, this study elucidates how filamentous and enzymatic DMT proteins govern sperm function through divergent mechanisms, which have implications for molecular diagnosis of male infertility.

History

Deposition	May 19, 2025	-
Header (metadata) release	May 27, 2026	-
Map release	May 27, 2026	-
Update	May 27, 2026	-
Current status	May 27, 2026	Processing site: PDBc / Status: Released

Map

• File: Download / File: emd_64679.map.gz / Format: CCP4 / Size: 1.1 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.087 Å

Density

Contour Level	By AUTHOR: 4.0
Minimum - Maximum	-14.100918999999999 - 25.268740000000001
Average (Standard dev.)	-0.000000000001078 (±1.0)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 672 672 672 Spacing 672 672 672
Cell	A=B=C: 730.464 Å α=β=γ: 90.0 °

Supplemental data

Half map: #1

• File: emd_64679_half_map_1.map

Half map: #2

• File: emd_64679_half_map_2.map

Sample components

Entire : doublet microtubule of mouse sperm flagella

• Entire: Name: doublet microtubule of mouse sperm flagella

Components

• Complex: doublet microtubule of mouse sperm flagella
  • Protein or peptide: Cilia- and flagella-associated protein 95
  • Protein or peptide: EF-hand domain-containing family member B
  • Protein or peptide: Cilia- and flagella-associated protein 53
  • Protein or peptide: Nucleoside diphosphate kinase homolog 7
  • Protein or peptide: Cilia- and flagella-associated protein 107
  • Protein or peptide: Cilia- and flagella-associated protein 141
  • Protein or peptide: Tektin-1
  • Protein or peptide: Tubulin alpha-3 chain
  • Protein or peptide: Tubulin beta-4B chain
  • Protein or peptide: Sperm axonemal maintenance protein CFAP97D1
  • Protein or peptide: Meiosis-specific nuclear structural protein 1
  • Protein or peptide: Tektin-2
  • Protein or peptide: EF-hand calcium-binding domain-containing protein 3
  • Protein or peptide: Tektin-3
  • Protein or peptide: Sperm-associated antigen 8
  • Protein or peptide: Tektin-4
  • Protein or peptide: Cilia- and flagella-associated protein 161
  • Protein or peptide: Ciliary microtubule inner protein 1
  • Protein or peptide: Ciliary microtubule inner protein 3
  • Protein or peptide: Piercer of microtubule wall 2 protein
  • Protein or peptide: Ciliary microtubule inner protein 4
  • Protein or peptide: Ciliary microtubule inner protein 2A
  • Protein or peptide: Luteinizing hormone beta
  • Protein or peptide: Cilia- and flagella-associated protein 68
  • Protein or peptide: Protein SPMIP1
  • Protein or peptide: Cilia- and flagella-associated protein 77
  • Protein or peptide: Cilia- and flagella-associated protein 144
  • Protein or peptide: Protein SPMIP3
  • Protein or peptide: Cilia- and flagella-associated protein 90
  • Protein or peptide: Sperm-associated microtubule inner protein 4
  • Protein or peptide: Ciliary microtubule inner protein 2C
  • Protein or peptide: Protein SPMIP7
  • Protein or peptide: Sperm microtubule inner protein 8
  • Protein or peptide: RIB43A-like with coiled-coils protein 1
  • Protein or peptide: Dual specificity phosphatase 21
  • Protein or peptide: EF-hand domain-containing protein 1
  • Protein or peptide: Sperm microtubule inner protein 6
  • Protein or peptide: Sperm microtubule inner protein 11
  • Protein or peptide: Protein SPMIP9
  • Protein or peptide: Tektin bundle-interacting protein 1
  • Protein or peptide: RIB43A-like with coiled-coils protein 2
  • Protein or peptide: Tektin-5
  • Protein or peptide: EF-hand domain-containing family member C2
  • Protein or peptide: Stabilizer of axonemal microtubules 4
  • Protein or peptide: Testis-specific serine/threonine-protein kinase 6
  • Protein or peptide: Cilia- and flagella-associated protein 20
  • Protein or peptide: Ciliary microtubule associated protein 1A
  • Protein or peptide: Parkin coregulated gene protein homolog
  • Protein or peptide: Cilia- and flagella-associated protein 45
  • Protein or peptide: Sperm-associated microtubule inner protein 5
  • Protein or peptide: Tektin-like protein 1
  • Protein or peptide: Cilia- and flagella-associated protein 52
  • Protein or peptide: Enkurin
  • Protein or peptide: Sperm-associated microtubule inner protein 10
  • Protein or peptide: Protein Flattop
  • Protein or peptide: EF-hand calcium-binding domain-containing protein 6
  • Protein or peptide: Cilia- and flagella- associated protein 210
  • Protein or peptide: Cilia- and flagella-associated protein 276
  • Protein or peptide: Piercer of microtubule wall 1 protein
  • Protein or peptide: Sperm microtubule inner protein 2
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: ADENOSINE-5'-TRIPHOSPHATE

Supramolecule #1: doublet microtubule of mouse sperm flagella

• Supramolecule: Name: doublet microtubule of mouse sperm flagella / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#60
• Source (natural): Organism: Mus musculus (house mouse)

Macromolecule #1: Cilia- and flagella-associated protein 95

• Macromolecule: Name: Cilia- and flagella-associated protein 95 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 26.633035 KDa

Sequence

String:

MGTFDSQCHE CFESKERWYE IGPTDLLERK GSLTLRSHHK KYSEPVLVYS WHRNREAFPK DYDIESLETV KNLCMSTYKR FGSDSPRWM SEAREQMAQV LVNKDLVEKK KTGLLDEETL CPGILERDPE LPPTGFRDVP MESPPDPRKP CFSTTYSEDF T PQYEYPPP ACPRREEYSI VHRKCRSQFT DLNGSKRLGI NTWHDESGIY ANSEAKQKLY ALARNPIVPF

UniProtKB: Cilia- and flagella-associated protein 95

Macromolecule #2: EF-hand domain-containing family member B

• Macromolecule: Name: EF-hand domain-containing family member B / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 95.891961 KDa

Sequence

String:

MCSFVRVGSP KPLQTSASPL EMSSLRRTRA PEISELGLTP EQKDDIRDRV LRGSKSPTEL GFDLRLEQDR KWRERMGSSE AKSPPCHAL GVGLERHTIS GTPTEMGNLG LHKGSAFQGS KPLGVLPGRV GPENKGLPPR LRYGGTLHPP FSTVHASPLA A ESRRRPLA WGSAWTDAVV EKQPVVGLEL RKEPEKEPTC VVMNPYPEMP PKEVDIGLPQ TQESDEAKNT EPLIGLVREP SE CPFAQQP EEKKEPGSTE PGVEPPGNIR PIYSGKFFDR VPCWPSAGKV KPVGYRVATC LTEKLPRLMT PPEAKKYFNF RYP PAGAER VFYGRANDPQ IAPYLTHGLR SKISIPMGSL INPQPITTFQ QKIKDKKESI YFSHQRAPLG KSHDQTPGLP KGMD VINTT LGTPTIRELS VRDTVNPSKS FEDVLKEGQE GHDLYTVSHN DYFAGEAKNR KYNPASFHRF NLYGIPTPHF NDGRT MAKA LHWLHELQME RGAKIVSKRV DDFKEKFQHK LGKVLDPIAE TMNVPPGHTF GSCLHPEEYG AGDLIHYRSP DEYLRG KDH QRAVVAAARH HLKKFNHQNF DTLQVAFRHY DKKGDGVIDR AELHEACVQA NLHLDKMLLD HLFDYCDVDQ DGLINYL EF ANFLNWKDRI PLKEHEKRVV VKGKKPDCEN VTDTSMGEAE PSLLINPEDI VPKEPGSSEE TLRTIQRPGD KVSHQYKT T SSEINAVVGA VPSMCHPIFG VPTIRSDISA PRIRRVSDMN NYGDEGNAYS LLHPSIFSQK GVFERDFFKT RSKEEISDI LTNIGVKLSK EEFENVWNLA SKKHQRGEVC VETIRNVLDE LLHADLVKCK TAM

UniProtKB: EF-hand domain-containing family member B

Macromolecule #3: Cilia- and flagella-associated protein 53

• Macromolecule: Name: Cilia- and flagella-associated protein 53 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 62.036609 KDa

Sequence

String:

MYSQQFGTVP REFKGPTPKA VIIRAKPPKA QRAEQHLKRI QRSYHKYHTT LASIKSNEEN RLKCDWIQRN NHKTFDSLVQ ARVQDAMQG FVINTEERRN KLRELLASEE NEYFSEMQLK GETIEEKKDK MRERTKLLRE KKEKERQEFV AEKLDQQFRE R CEELRTKL ASIHEKKVVE ERNAQIEFNK ELKRQKLVEE HLFARLWEED RLAKERREAQ EEKRQRELVQ NTRLGLDAQV TS IQAQRQG ARRMKEEEAR ILEQNKAQIK REDEQEKLQK QKRRQETRSS LKKAVQDKIE SMQREYREDL DLNMKLVGRA LQD LQDEAD KKKQKREEMG REQKIYNDYL MQRREEEKAQ EKELNRLLED IKAKKLAEKD RELALQRAAR KQLMNEVMNT RKLQ VQERL QRKLREQEEL ALHEQRISES LKVLHQEDME DFARRCALAE EYRNQLQMQI AHQQQAREAE KEEERQEFEA GLAAN KACL DKIQRILSEN QALSQNVHPM RRGYPDKPPL

UniProtKB: Cilia- and flagella-associated protein 53

Macromolecule #4: Nucleoside diphosphate kinase homolog 7

• Macromolecule: Name: Nucleoside diphosphate kinase homolog 7 / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO / EC number: Hydrolases; Acting on ester bonds
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 44.489828 KDa

Sequence

String:

MRACQQGRSS SLVSPYMAPK NQSERFAFIA EWYDPNASLL RRYELLFYPV DGSVEMHDVK NRRTFLKRTK YEDLRLEDLF IGNKVNVFS RQLVLIDYGD QYTARQLGSR KEKTLALIKP DAVSKAGEII EMINKSGFTI TKLRMMTLTR KEAADFHVDH H SRPFYNEL IQFITSGPVI AMEILRDDAI CEWKRLLGPA NSGLSRTDAP GSIRALFGTD GVRNAAHGPD TFASAAREME LF FPSSGGC GPANTAKFTN CTCCIIKPHA ISEGMLGKNL IAIRDACFGM SAIQMFNLDR ANVEEFYEVY KGVVSEYNDM VTE LCSGPC VAIEIQQSNP TKTFREFCGP ADPEIARHLR PETLRAIFGK TKVQNAVHCT DLPEDGLLEV QYFFKILDN

UniProtKB: Nucleoside diphosphate kinase 7

Macromolecule #5: Cilia- and flagella-associated protein 107

• Macromolecule: Name: Cilia- and flagella-associated protein 107 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.06251 KDa

Sequence

String:

MAMLSTSVVP EAFSTPGWQI EKKYSTKVLL GNWVEERGKF TKAIDHTPQC IYRKEYVPMP DHRPDFVSRW YSKSKMEGLP YKHLITHHQ EPSHRYLIST YDDHYNRHNY NPGLPALRTW NGQKLLWLPE KSDFPLVAPP TNYGLLEQLQ QKWLASKTSL K ESIYTTSY PRLPVCAMSR REHAIPVPHP RLQPIPRF

UniProtKB: Cilia- and flagella-associated protein 107

Macromolecule #6: Cilia- and flagella-associated protein 141

• Macromolecule: Name: Cilia- and flagella-associated protein 141 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.278145 KDa

Sequence

String:

MSAEKMTKLE ENLQRAVALK KTVDRWRNFH IHCMWQTTLD QRRNLFAALR MKDTKEQELA LSNKQLLVVR QAALHELFEK EYQQYQQEL NQMGKAFYEE RL

UniProtKB: Cilia- and flagella-associated protein 141

Macromolecule #7: Tektin-1

• Macromolecule: Name: Tektin-1 / type: protein_or_peptide / ID: 7 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 48.713035 KDa

Sequence

String:

MAKLLQSPPR FLPEEWYIAN KSQYHRAEAQ RSQSERLVAE SQRLVEEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVNQTD DLLTYKTRLE RSLESYKEPL HITEKCLEYR EKRVGIDLVH DVVEQELQKE ADIIHGVMNL LIRTLEESTE Q IRLNRSAK YNLEKDLRDK FTAITIDDVC FSLNNNSPNI NFSEKVVRIE PNSVSLEDWL DFSNANVEKA DKQLNNSTAL KT LVDQILS QTANDLRRQC EVVDEAFING LKETKDARNK LADHLAKVME EIASQEKNIM ALENAITQQE GPAKVAHTRL ETR THRPNV ELCRDIAQYR LIKEIQEINH NVARLKETLA QAQTQLKALY RRQLALQEEI QVKENTIYID QVLCMEMRKS IPPR DGDDH GAWEGGIRAE AIC

UniProtKB: Tektin-1

Macromolecule #8: Tubulin alpha-3 chain

• Macromolecule: Name: Tubulin alpha-3 chain / type: protein_or_peptide / ID: 8 / Number of copies: 167 / Enantiomer: LEVO; EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.012359 KDa

Sequence

String:

MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVVDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIVDLVL DRIRKLADLC TGLQGFLIFH SFGGGTGSGF ASLLMERLSV D YGKKSKLE FAIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCM LYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GVDSVEAEAE EGEEY

UniProtKB: Tubulin alpha-3 chain

Macromolecule #9: Tubulin beta-4B chain

• Macromolecule: Name: Tubulin beta-4B chain / type: protein_or_peptide / ID: 9 / Number of copies: 170 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 49.877824 KDa

Sequence

String:

MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEAEEEVA

UniProtKB: Tubulin beta-4B chain

Macromolecule #10: Sperm axonemal maintenance protein CFAP97D1

• Macromolecule: Name: Sperm axonemal maintenance protein CFAP97D1 / type: protein_or_peptide / ID: 10 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 19.811641 KDa

Sequence

String:

MNNSLDYLAY PVIVSNHRQS TTFRKKLDFG HYILHKNRVQ IVKPAVDTKP PMAHTHYILK LSKIQGEQKR IDKIEYENRQ LCQKIANAY RGPAKVDCWN EYLSKSLNRE SRNRELVRIT MENQGILRRL SDRKPNYDRR SSEMDWQNSR RYIRNTTRYL L FQED

UniProtKB: Sperm axonemal maintenance protein CFAP97D1

Macromolecule #11: Meiosis-specific nuclear structural protein 1

• Macromolecule: Name: Meiosis-specific nuclear structural protein 1 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 60.353012 KDa

Sequence

String:

MATKKRALSF SEKHQKLVDE KFRKSLNIQV MNKLERQAKN QVVQNENDEK VERQRFLRVL QNEQFELDME EAIQKAEANK MLRDRQLEQ EERLANELAR LKHESLKDKK MRQQVRENSI ELRELEQKLK AAYMNKERAA QIVEKDAMKY EQMKRDAEIE R IMMEEHDR LLKEESAKQE RRNKERAQYY LDLEKQLEDQ ERRKQEAYEQ LLKEKLMIDE IVRKIYEEDQ VERQQKLEKK NA IQKYIEE FQRAQDFWRQ KKREEMEEEN RKIIEFANIQ EQREGERMAR VHEIEEKRVQ RQNLLMKQLE ETLRQRDDLE QVR QELYQE EQAEIIKLKV KEEAELRLRR QREMKQDFED QMALKELILQ AAKEEEETFK KAMLAKFAED DRIELMNAQK QRMK QLEHK RAVEKLIEER RSQFLADKQR ELEELQLQQR RQGCINEIIE EERLRLLKEH AAKLLGYLPK GVFKREDDVD MLGEE FRKA YQKRDGV

UniProtKB: Meiosis-specific nuclear structural protein 1

Macromolecule #12: Tektin-2

• Macromolecule: Name: Tektin-2 / type: protein_or_peptide / ID: 12 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.385066 KDa

Sequence

String:

MATLSFKPSE RYRLSDWRTN SYLLSTNAER QRDASHQIRQ EARILRNETN NQIVWDEHDN RTRLAERIDT VNRWKETLDK CLTDLDAEI DSLAQAKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV RDPVEEELLK EVEVIEATKK VLQEKISQAF Q HLCLLQEI RQQLNSDHRD KMETLEIDRG CLSLNLTSPN ISLKVNPTRI PKDSTTLQQW DEFTRFNKNR AEAEMKASIE LR EAIALAI AQTNNELDAQ RVATEFTFRK RLREMESFYS ELKWQEKNTL EEIAELQGDI RRLEEDLRRK MMNLKLAHTR LES RTYRSN VELCRDQTQY GLIDEVHQLE ATINTMKQKL AQTQNALDAL FKHLARIQAD IACKTNTLLL DTKCMDTRRK LTVP AEKFV PQVDTFTRTT NRTLSPLKIC QLELT

UniProtKB: Tektin-2

Macromolecule #13: EF-hand calcium-binding domain-containing protein 3

• Macromolecule: Name: EF-hand calcium-binding domain-containing protein 3 / type: protein_or_peptide / ID: 13 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 49.795141 KDa

Sequence

String:

MAVSEIKATM KLKPLKAPVF HKRDKDLRGS FSDHLKHKES KLSPAQLEAF RNAYNFFTKD RTGCIDSHGL ISTIAKLGMN LNTYDIYNE LKCADLDRDG KINFSDFINV LTDKKLFLKA VVPEKKICLD LANNPGILLF EILSKFVETS SLHRKDIIEL V SYFRKRFQ ESHSEIMWSS YGRRGLKSEI CSPPRSSTAA FANSARISIM KERDLYKFLE ALKRCNLRTD SPYSKIPVFP LF PDVDGTV MGKPFKDTQK IEMLRRKEPL TFFEDYFFNK RDWKTQAMNV KPLKSASGYS DDILAIDHLF KKKQHWTVTD AAA IKQHVK KATESYNLGI ALDHRKEMLN LWKKIRGDLV GIESNNESFY NTFSTYTWSW NVCQELLSAK DLRLHDASMN KSSP SNSGL SSPSDFSESD PETGRKRKRK SSRGFRQ

UniProtKB: EF-hand calcium-binding domain-containing protein 3

Macromolecule #14: Tektin-3

• Macromolecule: Name: Tektin-3 / type: protein_or_peptide / ID: 14 / Number of copies: 16 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 56.754777 KDa

Sequence

String:

MELLGSTLTA TYAHPPPASA SFLPAIGTIT SSYKDRFPHR NLTHSLSLPW RPNTYYKTAY NYPTLAPYSS RSQRVCESTM LPFVSNRTT FFTRYTPDDW YRSNLVSFQE SNSSRHNSER LRVDTSRLIQ DKYQQIRKTQ AHSTQNLGER VNDLAFWKSE I THELDEMI GETNALTDIK RRLERGLIET EGPLQVSREC LFHREKRMGI DLVHDEAEKE LLAEVDTILC CQERMRQHLD KA NAQLASD RSAQHELEKD LSDKQAALRI DDKCQHLRNT SEGVSYFRGV ERVDATVSVP ETWAKFTDDN VLRSQSERAA SAK LREETE NLLIVTANEM WNQFNKVNLA FTNRIAETVD AKNKIHTHLT KTLQEIFQIE MTIESIKKAI KEKSAFLKVA QTRL DERTR RPNVELCRDM AQLRLVNEVY EVDETIQTLQ QRLRDSEDTL QSLAHTKATL EHDLAVKANT LYIDQEKCMS MRNSY PSTL RLVGYC

UniProtKB: Tektin-3

Macromolecule #15: Sperm-associated antigen 8

• Macromolecule: Name: Sperm-associated antigen 8 / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.591805 KDa

Sequence

String:

METTESTEGS LSRSCDVQPS SERLDTPSEP VPSSSSSPRS TAPAEAPAQY SVLTEPSSDS LYGAPCPPAH HRGHGFGFQP FYVSCIPQD PCNMADLSSR ADPTSSYPCH SSVHGSGSGT CGLGQSSEPS QGSGPTSGPA PASVPSLVSG PDSASGPDSS A SGPALASG PGPADPGQGP KFSTCIPQGY RCIPVDLAPD YNAWCQHLHW KPQRSWEPLQ VSEPGVRGPY KPPEPGALGP CE PCEPCEP PEAESEETLC KARPRGQCLL YNWEEERATN QLDQIPPLQD GSESYFFRHG HQGLLTTQPQ SPMSSSTTQR DSY QLPRHI CQPLRGKREA MLEMLLRHQI CKEVQAEQEP ARKLFETESV THHDYRVELV RAAPPASTKP HDYRQEQPET FWIQ RAARL PGVSDIRTLD TPFRKNCSFS TPVPLSLGQP LPYELESGPH QVGVISSLAC QGGGQGCGRT KTTPI

UniProtKB: Sperm-associated antigen 8

Macromolecule #16: Tektin-4

• Macromolecule: Name: Tektin-4 / type: protein_or_peptide / ID: 16 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 52.121449 KDa

Sequence

String:

MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK YLRDEWFQNS YARYHQAFAD RDYSERQRHE SGQLVAETG ALAQRTQLDS TRKVGERLED MHCWKSELQR EIDELSSETD LMMAQKLRLQ RALDATSVPY SIATDNLQCR E RRQHPDLV RDYVEVELLK ETELIRNIQE LLKRTIGQAV DQIRLNREHK ESCEMNWSDK VEVYNIDDTC SRYTNESTQV QF YPHSSKF EESASTPETW AKFNHDNLLR AERERLASVN LRKLIDCILR DTAEDLRLQC DAVNSAFSSR CQELDDSLQK LQY HLRKTL TEITDQEHQI AALKQAIKDK EAPLRVAQTR LYQRSHRPNV ELCRDNAQFR LLSEVEELNM SLRALKEKLQ DAEQ ALRNL EDSRMSLEKD IAVKTNSLFI DRQKCMTHRN RYPSVLQLAG YQ

UniProtKB: Tektin-4

Macromolecule #17: Cilia- and flagella-associated protein 161

• Macromolecule: Name: Cilia- and flagella-associated protein 161 / type: protein_or_peptide / ID: 17 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 34.433383 KDa

Sequence

String:

MAQNVYGPGV RMGNWNEDVY LEEERMRHFL EKREKGELLI QRNRRVKKNI LRPMQLSVSE DGYVHYGDKV IIVNPDQVLG EEAGKFMRG DLSLCMSPDE VKAQLSDDLE IPCGVSAVQT IAPMGRNTFT ILSDGANSCE MGQVVVYGQN FCLGIAAGLE G KMLYLTSD HRTLLKSSLK SGLQEVTLTD EVTHLNCWQA AFLDPQLRLE YEGFPVRANE KIVIYHRHTN RALAVHRNLF LR TYFGKEM EVVAHTYLDS HKVEKPKNQW MLVTGNPRNK SNTMLDISKP ITEDTRALEQ AMGINT

UniProtKB: Cilia- and flagella-associated protein 161

Macromolecule #18: Ciliary microtubule inner protein 1

• Macromolecule: Name: Ciliary microtubule inner protein 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.609958 KDa

Sequence

String:

MAQKPLSTTA AERMNLVAQD EIWKYRLRAE SEARQNWPAK WGYLTTSMKE LLEGEEEPQT PKPKPELPSH FYVRPVSPMD KHIKILPSP PVPKTTQGFI GWRSGKPALY CLEKYSEVCS CKGAYARELC WPEQGVH

UniProtKB: Ciliary microtubule inner protein 1

Macromolecule #19: Ciliary microtubule inner protein 3

• Macromolecule: Name: Ciliary microtubule inner protein 3 / type: protein_or_peptide / ID: 19 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.717516 KDa

Sequence

String:

MKGDRERQAD LSPREGPSVP RPVLLQTEPR SVFPGDTSRP WSPAALPARA PGDRWCGRRR RSPGNASYLD SDISEKSRSA SEEEKSHAL GACHCAENAF QDSQKSLVPS RGQKMTSGTK AKVPLTALSQ KWKRDREQTL EAAYVPVVVD PRGQNPDTSI R FSFYNSQY SNSLNPFYTL QKPTCGYLYQ RETDHTRKRF DVPPANLILW RT

UniProtKB: Ciliary microtubule inner protein 3

Macromolecule #20: Piercer of microtubule wall 2 protein

• Macromolecule: Name: Piercer of microtubule wall 2 protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.728513 KDa

Sequence

String:

MARETDCDLD KKTSLTSDAE MRPEPPALCV NPGNPVFSCM LDPKTLHTAT SLSKAQMIMY KTSASQYGAF SPRPFFFPCK FLPQEQAFT EHLKTTGFYQ NNSLNVGPDR TRTIDSPNYQ HTL

UniProtKB: Piercer of microtubule wall 2 protein

Macromolecule #21: Ciliary microtubule inner protein 4

• Macromolecule: Name: Ciliary microtubule inner protein 4 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 30.278754 KDa

Sequence

String:

MELSHRQGTT TLTRTHPNDK EGQQDMNSFR ANHSSLDNSK FKYHARLSQS PLGSSLGQGY LETPPLPPTP TCRTSLAMNS HPEDLKKGA SRSSSRDARE TFREGCVGEE GQDSRSPEQR TVPLSKKDSV IPENIRHKFG SKMVDQLISE DQARQAIGEM F EGQKRPSS WPSRTQSPMQ ASSIFSDYYD LGYHMRSNLF QGPPQETKSL MKASYTPEVI EKSVRDVEHW HGRKTDDLGR WH RKNAMNM NLQKALEEKY GEKSRSKAK

UniProtKB: Ciliary microtubule inner protein 4

Macromolecule #22: Ciliary microtubule inner protein 2A

• Macromolecule: Name: Ciliary microtubule inner protein 2A / type: protein_or_peptide / ID: 22 / Number of copies: 16 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 36.659566 KDa

Sequence

String:

MTATQKHNLF TPEPHYIPGY AGFYPQLRYQ VGNTYGRTTA QLLTDPSVQK SPCSVLSPMT KPKFIEDFSK SKPPWIPCRD LREPYIPHY TSLKPYKNFE ILGQLPRQDV DTQGPPQVEN RQGPLTAGFM PYPPYPACPP GRKGEARDLG HPGLLLAYGE E AWKDAAPL QDTPGKNNQL YHCRRDEYLP PHPPQETLDV GRFQRLPQLD HPNLIQRKAI SGYAGFVPRF AWVMGMNYRD GV TQAMDDF DKNQFVFRHP VCALGERLPR THWPNTTIYR SQGLIPFYMG FIPSMQDNYA LTFGNSTRRA YQKELDRRSH TL

UniProtKB: Ciliary microtubule inner protein 2A

Macromolecule #23: Luteinizing hormone beta

• Macromolecule: Name: Luteinizing hormone beta / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 34.424383 KDa

Sequence

String:

MAGRTLALRY GPPWSPISET EVLGTWPNWH LTSSGVAHHW IPPASFPLPT LQSMVKAPLP AVSKVISRWE TTSQSTYTPK THGGPCAQP KAPEHKDPRR TLGIKSLAEK LRRHEGRGVP LNTKCQISET KAQYKGWPDV DQSAPLFAEP QPPELADHHR G GPSQALIP WTKNPEMAGQ PFTVNKMGIL DRLQPYQTIT ASDFTRNVSG YPHQKPVMGL SWPRKASHRD RASLPRVRPA RP RPSQPYL GALSLTRESY GPLVHPLCGM DRICPVHASW GSPHRKPVLG LYSVPKAYCT ENSRYGSARA ELL

UniProtKB: Luteinizing hormone beta

Macromolecule #24: Cilia- and flagella-associated protein 68

• Macromolecule: Name: Cilia- and flagella-associated protein 68 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 19.552668 KDa

Sequence

String:

MAVSCSLNHS TYLQRQNLVC YLRNPHYGSL IYADGHGEVW TDWNDMSKFL QYGWRCTTNE NSYSNRTLVG NWNQERYDLK NIVKPKPLP SQFGHAFETT YDANYSRKKP QSTHRFKREP HWFPGHQPEL DPPHYKCTAK STYMTNYSEP QPTHYSCCVY D PSVSQS

UniProtKB: Cilia- and flagella-associated protein 68

Macromolecule #25: Protein SPMIP1

• Macromolecule: Name: Protein SPMIP1 / type: protein_or_peptide / ID: 25 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.439783 KDa

Sequence

String:

MSRQLNMDTL RQNFWKEEYL KEMMLRYEWQ RKYGTLVKAK QKAKAASRLP RKLPTLLPQA SVAPPPPASK TTPSKAPSPA PEPLFLSDM YPVAPNTKAL LYEGISHDLQ GRYQYLNTRK LDLPETRYLF PITTNFTYGW QLGPPTKQEL VSCKMCRIES F FRKNGAFA LLDPRDLAL

UniProtKB: Protein SPMIP1

Macromolecule #26: Cilia- and flagella-associated protein 77

• Macromolecule: Name: Cilia- and flagella-associated protein 77 / type: protein_or_peptide / ID: 26 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 32.78602 KDa

Sequence

String:

MPDPAKPGKD LTAWKKKKQP VHRTVSQICP PPRRPLTVVD IRTGMENERL GVVRDSMFQN PLIVKAELGK PRERSCSLPG INFNYGLYI RGLDGGVPEA IGHWNVFKQQ PTCPHELTRN YIAMNRGAVK AGLVTARENM LYRELNDIRI NDQEDRRQKE P PPIPPNMT FGIRSRPSTP FFDLLQHRYQ QLWVQEQKAT QQAIKMEKKQ KVILGKLYET RSSQLRKYKP PVKLDALWHM PH FKKVASH LATFPTEADR QRALKAHKEE YAVRQGTLRM GNYTHP

UniProtKB: Cilia- and flagella-associated protein 77

Macromolecule #27: Cilia- and flagella-associated protein 144

• Macromolecule: Name: Cilia- and flagella-associated protein 144 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.155242 KDa

Sequence

String:

MAGRVGQMKN QDEVHQNQIL RELFLKELRA QKLYTQYHVN PLRKVHTITR KPMSWHDNLE EPEDAKFLNL IHHAAQGPKK KYSETQTEA QEIGWDPNPL INPDRQDHRL NHFRVYHDIT LYKAKLWSLG EDDHQK

UniProtKB: Cilia- and flagella-associated protein 144

Macromolecule #28: Protein SPMIP3

• Macromolecule: Name: Protein SPMIP3 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.001305 KDa

Sequence

String:

MSTIRLREFV ERRPSIPPRL YITHQGRDIK GYYPGQLARL HFDYSGRKAP RPLIDLTIPL KSTTPYQPQL DQQTLIRSIC SRRLSRPTD LWHNETSYQR DYSLPFYESG WDRKLGTISL HPRPVNSVPE VYCCGGERSS YARSTF

UniProtKB: Protein SPMIP3

Macromolecule #29: Cilia- and flagella-associated protein 90

• Macromolecule: Name: Cilia- and flagella-associated protein 90 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.886428 KDa

Sequence

String:

MEELAKKERR AMDPGGLKKE GKVEEEAGKE EGREEEGGEE EEVTSETLRG KPRPLPISAL PAFSYIPPRH QGPKERSYFS REGQTGIVS LYDCVFKRRL DYNQKLHRDD REHAKNLGLH INEEEQERTV PVLMSSVYGK RINQPIEPLN RDYGHVSHVK T DFYRKNEI PSIKGPGFGH INPA

UniProtKB: Cilia- and flagella-associated protein 90

Macromolecule #30: Sperm-associated microtubule inner protein 4

• Macromolecule: Name: Sperm-associated microtubule inner protein 4 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 67.755727 KDa

Sequence

String:

MEVIHGRPHC CRNLEGADIL SNTFYSNGLH TPYETTIRPT ASQARYQELR EALPQCRLRW GADREYGGVL PVSLPEEHRP KCEPPRLMS KGHQHYGFGG EIWPKKLPIE QYYYMTQNKK SDVYGNDSLL PKPPNSAVKE ICSPYPIEHP YHTHISRGSV F PTFTSPKD LYTGIKARTQ QPFPPTVPTK ACDTTILKTR GNPYRYELLD FPMDSKKKAL TWPGQGVYYD FPKFVEKNKP VF YPKPPKT FAPNSSVNPW DTMSSAKDAN IQRNLERSHW LTSYAHDFTG LGPMSPLELD DYHEKELAEL TGQIGFDPQP QEK FHPALK TPRPLDGRIA RLTQNQRPLE ATVQIPPPCP DCTPRVLCAF HTFIPTSAEI MAMNNNLLSG ITHKNQDVEE KIKE EQGLM STCPLPTCYE SKDLTSLYDV QSFPKITDTK KTDDLYWRQL EMKPLPISCS KSNHYIDYEP LKSAYRDPYA MCPNP VRLS KSNILQNKTD TADFTFDNFL SKPEFLGMNM ESNEETRPLL DWIPRAGVPK HHSNLRNLRN TFSKSMAQKR LHNSIQ EEQ KDLRDKLQCG MRHQFFGYNG HHFYN

UniProtKB: Sperm-associated microtubule inner protein 4

Macromolecule #31: Ciliary microtubule inner protein 2C

• Macromolecule: Name: Ciliary microtubule inner protein 2C / type: protein_or_peptide / ID: 31 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.097957 KDa

Sequence

String:

MAFRSAGTLM TEFNAAFVPP ALMPGYKGHV PGVASSFGSS YGNTTFKYFQ DLRNTGLEKS YALLSGGCFP TIFSPNSPLV LTDNSQNWD RWLHQPTYSR SNQDNGRTTE LTNFYKTVQQ QRKYYQDKTG ILSRVPYFVL PVKEPDRYPL PTDLPPLSEK S KWNLLRLS PDYKRTYQTF PSGKRVSSQE RQRRDLYFEF RA

UniProtKB: Ciliary microtubule inner protein 2C

Macromolecule #32: Protein SPMIP7

• Macromolecule: Name: Protein SPMIP7 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.476312 KDa

Sequence

String:

MDMEVKTMPG KISIPRRFIF SEGKAIEHPD YPHYSNLLQK MNMPSVKGLE DRHNCVRFEK KCNPTFLKFH PYPPSVLPDY HLHYPYPPP YGRAYPLAPL RDDVPLGDPC SGFMSPGGDA NLKPNIGRAI PNLVSFHDVK PQNRVPRPDK GFQTTIKRQT I LAEELKQD RRWNSRKVPD ISIKAKLGGW TSPMKVVPVP VHEHEVGTLS RIYTFDEEAI STDDSEPLVQ LDKKYNIKDS FY KSSTQKA YEDVPWDKML PPKLDPEETT VEKAADHISQ CFSLKRYERL PAITQMVGGL WDRFQTRLFS APAKPINFVS PST RSKYIP LYTGHVQSTD ADNVDNPYGD IKSVASPRHS KLQYTNSSRS ANIPGYTGKV HFTATHPTNS NIPSREPSAD SEMN RLLLQ EMRVDRFRHQ GPMSQMVTTV KPYNPFNKKE KETLEY

UniProtKB: Protein SPMIP7

Macromolecule #33: Sperm microtubule inner protein 8

• Macromolecule: Name: Sperm microtubule inner protein 8 / type: protein_or_peptide / ID: 33 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.985697 KDa

Sequence

String:

MAQIIDLVPW DECSAHLYAS PAVLLPLERV RHPLAGVKHQ LYHPALPSLR RMDMDTVKGC LSDEHCQSST YFSKDDFNKA HFTLLGVPN KPLQCLDFTA TGQKLCHKYR GGKMIPIAPG INRVDWPCFT RAIEDWSKFV SRSEEFKLPC ANKRVEGFSG Y AVRYLKPE VTQNWRYCLN QNPSLDRYGQ KPLPFDSLNA FRRFGSHYSR INYLTPWH

UniProtKB: Sperm microtubule inner protein 8

Macromolecule #34: RIB43A-like with coiled-coils protein 1

• Macromolecule: Name: RIB43A-like with coiled-coils protein 1 / type: protein_or_peptide / ID: 34 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 45.03216 KDa

Sequence

String:

MYKLDLVSDP RELAAIEARR NREKERQSRF FNVRNRVMGV DVEALNYQVE ERKFREAIER NKDIAYGTKH AHYDLVAQML EKEEAERAY RLSKRVQDFR EQRQQQYKNG HEFDFWDPDQ FQEFRVPYYE KDAYFGPASM QYFFGEDLER ASHVRMQQEQ M RYNLEKQL QEQQAAREEE ARAALLSDQL RLAADTRAAE LARLEESCRA AMRTAMANAN KAQAAKQALQ QRREQQRQQE AN LTEVKKQ VTSDLLTENP QVAQRANAPH RVLPYCWKGM SAEQRAAIRK TQETQRQEKK EQRQAEKLVE AEWGRQNKRL AEA ALELEE QERELCAEFR RGLGSFNREL AKEQQAQQNY LNSVIYTNQP TAHYYLQFNT SSR

UniProtKB: RIB43A-like with coiled-coils protein 1

Macromolecule #35: Dual specificity phosphatase 21

• Macromolecule: Name: Dual specificity phosphatase 21 / type: protein_or_peptide / ID: 35 / Number of copies: 4 / Enantiomer: LEVO / EC number: protein-serine/threonine phosphatase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.527574 KDa

Sequence

String:

MTTASCIFPS QATQQDNIYG LSQITASLFI SNSAVANDKL TLSNNHITTI INVSAEVVNT FFEDIQYVQV PVSDAPNSYL YDFFDPIAD HIHGVEMRNG RTLLHCAAGV SRSATLCLAY LMKYHNMTLL DAHTWTKTCR PIIRPNNGFW EQLIHYEFKL F SRNTVRMI YSPIGLIPNI YEKEAYLMEL M

UniProtKB: Dual specificity phosphatase 21

Macromolecule #36: EF-hand domain-containing protein 1

• Macromolecule: Name: EF-hand domain-containing protein 1 / type: protein_or_peptide / ID: 36 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 75.235422 KDa

Sequence

String:

MGTNPVHGLP FLPGSSFTDS TKTAFHRSQT LNYRNGYAVV RRPTMGIGGD RLHYNQLSQA ELDELANKAP ILTYGPLKQA PLAEFVPAH VAFDKKVLKF SAYFQEDVPI SMEEHYRIRH VNIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRFTKNDM G DHYHWKDL NRGINLTVYG KTFRIVDCDR FTQDFLESQG IELNPSEKIP LDPYTQLRKE PVRKYVTPSD FDQLKQFLTF DK QVLRFYA IWDDTDSLFG ECRHYIIHYY LMDDTVEIRE VHERNNGRDP FPLLMNRQRM PKVLVENAKN FPKCVLEISD QEV LEWYTA KDFIVGKPLT ILGRTFFIYD CDPFTRQFYK DKFGMPDLPP VDVTKKEPPP VKQELPPYNG YGLIEDSAQN CFAL IPKAP RKDVVKMLMN DNKVLRYLAA LESPIPEDKD RRFVFSYFLA TDMISIFEPP VRNSGIIGGK FLGRTKVVKS FSPVD NPIY YSPSDFFIGA VIEVFGHRFV ILDTDEYVLK YMESNASQYS PEALASIQNR IQKPELPAPE LESKQATGEP MVQGTE ESK VQDLDALIDQ IHMHLKYNSY KENLRETFQM YDKDESGYVD RETFFKICET LNVPVDDSLI KELIRLCTHG EGRINYY NF VRAFSN

UniProtKB: EF-hand domain-containing protein 1

Macromolecule #37: Sperm microtubule inner protein 6

• Macromolecule: Name: Sperm microtubule inner protein 6 / type: protein_or_peptide / ID: 37 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.977191 KDa

Sequence

String:

MFLFSRKTKT PISTYSDSYR APTSIKEVYK DPPLWAWEAN KFVTPGLTQT MHRHVDPEAL QKMTKCAAQD YTYKSSISGH PYLPEKYWL SPDEEDKCCP SYLDNDRYNT WKTSPCSNYW NKYTGCLPRL SKDTGMESVR GMPLEYPPKQ ERLNAYEREV V VNMLNSLS RNRTLPQIVP RCGCVDPLPG RLPYQGYESP CSGRHYCLRG MDYCTTREPS TERRLRPLCS QQPTECVALR SP ARNAMCC YNSPAIILPV SQP

UniProtKB: Sperm microtubule inner protein 6

Macromolecule #38: Sperm microtubule inner protein 11

• Macromolecule: Name: Sperm microtubule inner protein 11 / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.528622 KDa

Sequence

String:

MAFFNLYLLG YQHYFQSKKR NTAQETNQKD EVPTRLPPII SEDGNYSVHQ NSHERYQEAV RKVLLKTFPN QVFRVPVTDA QNFSFWRSN IPGVRPEKAI PWIKTPHHCL IKSPMTRFVD HSHLNDKTFS LY

UniProtKB: Sperm microtubule inner protein 11

Macromolecule #39: Protein SPMIP9

• Macromolecule: Name: Protein SPMIP9 / type: protein_or_peptide / ID: 39 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.069139 KDa

Sequence

String:

MARVVRPQKN HVDLDIYQSS YMVDYKPFGK YKYSRVTPQE QAKLDAQLQS KEFYQPKPNP NPKLEEGYPA FRRPYMTALD LGVPGFFPP QERVTTRKDD GRFTTTCHYA YPASLALYLA QQDPYWLHQR ADFPCLMEPE RQPAPEVGKG YLLLPGCLCD H HQRVKVPI LNRWGPLMPF YQ

UniProtKB: Protein SPMIP9

Macromolecule #40: Tektin bundle-interacting protein 1

• Macromolecule: Name: Tektin bundle-interacting protein 1 / type: protein_or_peptide / ID: 40 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.987262 KDa

Sequence

String:

MENVRREATR PSVPSGTLEL YFPDHLYRND YVSLEGPRWA PAIKQAVRWK FTPMGRDAAG QVWFTGLTNS EPGDAWYKLP RALDTPYRE AHTRWHGCFQ SRQRGLPPAY TQHLREMAFW DPAITAQYLN SGPRWGCMQW RDRQIRGKEF VVTRNQFGAK L PWRSDYVP LLSLPQRPRF TAQDFRQRGL QRPCPAIGQP PPAFTPAL

UniProtKB: Tektin bundle-interacting protein 1

Macromolecule #41: RIB43A-like with coiled-coils protein 2

• Macromolecule: Name: RIB43A-like with coiled-coils protein 2 / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 45.328156 KDa

Sequence

String:

MEVAMSKDLQ QEANLAKKRY IDLCRQGRIF DARNRIIGGD TQAWDFQVRD QKIKEITDKA RHEAFAAEMK HNDKVMCMAH DREQRHRKQ LCRAINDFQQ NFQKPETRRE FDLSDPLALQ KELPARISDN DMRNTISGMQ KFMGEDLNFQ ERRRFQKEQS R EWFLQQHG EREKARADHL LAEHLHTQTR LKFDETAREL MKLEGSTRKE VCAAVKAFNK NQVVELTERK RQEKQQEQED NM TEITNLL HGDLLSENPR PVASSFGSHR VVLDRWKGMN REQLEEIWFT QKRQIQEKLR LQEEERQHSM DWDLRRIRKA HAS LLHERQ QQRLLREQRR ALDCSNLNLA RQQYLQKKQM NTASSSQPTE DYFSQFNTRS R

UniProtKB: RIB43A-like with coiled-coils protein 2

Macromolecule #42: Tektin-5

• Macromolecule: Name: Tektin-5 / type: protein_or_peptide / ID: 42 / Number of copies: 26 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 62.817535 KDa

Sequence

String:

MEFLGTTQTA SFCGPKKGCG LQALPPAGQE PVVQECYQPF HLPGYRYLNA WRPSVFHKIA TSQTIPEECS GIRRPPTILP SLRSALFCR YTPRDWDRSN DLQIRNAEAS RLWASRLTGD SLRIMQDKDQ LIHQMQEGTS RNLGQRLSDL GFWKSELCYE L DRLLTENS SMDTLKRRLE CAAEEVNCPL QVALECLYNR EKRIGIDLVH DNVEKNLIRE VDLLKCCQDQ MRKLAKRIDF QI RDNRDAQ HSLERDIEDK SSAQYIDENC FNLRSTSDSI SFFHGVEKFD GTVSIPETWA KFSNDNIRHA QNMRANSIRL REE AEHLFE TLSDQMWKQF TNTNLAFNAR ISEETDVKNK LQTQLAKILQ EIFQAENTIM LLERAIVAKE YPLKMAQTML ACRT RRPNV ELCRDVPQFR LVNEVFTIDD TLQTLKLRLR ETQDTLQLLV MTKSRLEHEL AIKANTLCID KDKCMSMRKS FPSTP RLTG YTCSAIGSGP YANHAPRISS GPCSGSALCK GPASCGGGAS CGGGASCGGH APCGSALCSH SVSRSGPGFA PVC

UniProtKB: Tektin-5

Macromolecule #43: EF-hand domain-containing family member C2

• Macromolecule: Name: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 43 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 87.758023 KDa

Sequence

String:

MALPFLPGNS FNRNIGKERF HKSQHWGFCN NVRMLVSENK PGVGGDLLYG QKIKPKHSVF PKGDGTDAPS WVAFDKQVLS FDAYLEDEI SDKRQEIFRI RYYKIYFYLE DDTIQVNEPE VINSGLPQGT SIRRQRIPYP PPNDDQFYTV YDFNINISVV F YGRTFKIY DCDPFTKNFL KKIGIKLNPP GQCPLDPYMK MRRETLEFVD PFRPYQSFDT LKRFIQYDGK VLRFFCLWDD ST SLFGDRR EFVLHYFLCD GTVEIREVLP SNSGRDAMSS FLRRGKLPKY GPPGIYQPGQ ITDRAVLNVY GGLSEWRADG YLL DKYQLG KVEQDFYTDQ DLSIGATINV WGRKVLLCDC DEFTKTYYRT KYGVDNFTPI SCKPPHLPKI ERKYPPYTGF GSEE DSFRS CVGLKPTPHR KNFKKFMELD SFGNISNILR YFGKLITHKC ADVDRIFVIA FYLSDDTISV FEPIENNSGN AGGMF LKRS RVKKPGQEVF KSEFSEYIKA EELYIGATVN INGYLFILLN ADEYTLNYME NNTDKFPYSN FELAIQKLKQ EKSKSR EIT QVFAAADYNH TKVVPYNTFR DILMSITMGK LIDQELITIA RHYRVPEIMD PDLAYLIARA HEKFKKNIFE NFDMFIY NC VYEDREKKGV LPTKDIRRMC KSSRLPLDDD FLDCLLSRFE DKDHQINYEI FFSVLNWRMN PTPDLQAPPY LKEKCEDV W VGMPSPIPVK YVRYLDFLID VYGLEDNML

UniProtKB: EF-hand domain-containing family member C2

Macromolecule #44: Stabilizer of axonemal microtubules 4

• Macromolecule: Name: Stabilizer of axonemal microtubules 4 / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 47.873078 KDa

Sequence

String:

MMGKLPLGVV SPYVKMSSGG CSDPLKFYAT SYCTAYGREE FKPRLGSHVG TGYKSNYRPL VSYQPHLDTL DNPAIGQQIR DTSKSVTSQ SYSPLEVPDG KQPLPWNLHQ TTSSYGREKL NPGPHSKEVR KVHFDTQDHG PQTITGLEPK EVPLIHQQQG K GSTEWENS HYGPRFMTSE YNSKYIKESP NHPDLLLKKT IGSKEETGFT EESTKNPIVF QPPSQAFPGD PVLHPGRSIT KS DYLPVTH PQGSDFLPVL SRGSDRDTGF SRVNERTLNP RVPTPAPQPA SMSHRSYQPP QRMQQTNVAL LGRESVGNKE PTG FTLNNP SYVRSSYEQD RDQRYLTTYN QGYFENIPKG LDREGWTRGG IQPQKAGAYA LSELNNHTLM DSTPNPTETL RHLH PHVGR TLASVDPFYR DMPHSSRYPA SS

UniProtKB: Stabilizer of axonemal microtubules 4

Macromolecule #45: Testis-specific serine/threonine-protein kinase 6

• Macromolecule: Name: Testis-specific serine/threonine-protein kinase 6 / type: protein_or_peptide / ID: 45 / Number of copies: 3 / Enantiomer: LEVO / EC number: non-specific serine/threonine protein kinase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 30.332943 KDa

Sequence

String:

MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP DFVNKFLPRE LSILRGVRHP HIVHVFEFIE VCNGKLYIV MEAAATDLLQ AVQRNGRIPG SQARELFSQI AGAVRYLHDH HLVHRDLKCE NVLLSPDERR VKITDFGFGR Q AHGYPDLS TTYCGSAAYA SPEVLLGIPY DPKKYDVWSL GVVLYVMVTG CMPFDDSDIA GLPRRQKRGV LYPDGLELSE RC KSLIAEL LQFSPSARPS AGQVARNGWL RAGDSG

UniProtKB: Testis-specific serine/threonine-protein kinase 6

Macromolecule #46: Cilia- and flagella-associated protein 20

• Macromolecule: Name: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 46 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.781389 KDa

Sequence

String:

MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

UniProtKB: Cilia- and flagella-associated protein 20

Macromolecule #47: Ciliary microtubule associated protein 1A

• Macromolecule: Name: Ciliary microtubule associated protein 1A / type: protein_or_peptide / ID: 47 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 27.690973 KDa

Sequence

String:

MAEEVWMGTW RPHRPRGPIM ALYSSPGPKY LIPPTTGFVK HTPTKLRAPA YSFRGAPMLL AENCSPGPRY SVNPKILKTG KDLGPAYSI LGRYHTKTLL TPGPGDYFPE KSTKYVFDSA PSHSISARTK TFRVDSTPGP AAYMLPVVMG PHTVGKVSQP S FSIKGRSK LGSFSDDLHK TPGPAAYRQT EVQVTKFKAP QYTMAARVEP PGDKTLKPGP GAHSPEKVTL NKPCAPTVTF GI KHSDYMT PLVVDVE

UniProtKB: Ciliary microtubule associated protein 1A

Macromolecule #48: Parkin coregulated gene protein homolog

• Macromolecule: Name: Parkin coregulated gene protein homolog / type: protein_or_peptide / ID: 48 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 27.763268 KDa

Sequence

String:

MPKRTKLLPQ QTFQVHQPRS LVSEGFTVKA MMKNSVVRGP PVAGAFKERP AKPTTFRKCY ERGDFPIALE HDSKGNKIAW KVEIEKLDY HHYLPLFFDG LSEMTFPYEF FARRGIHDML EHGGNKILPV IPQLIIPIKN ALNLRNRQII CVTLKVLQHL V VSSEMVGE ALLPYYRQIL PILNIFKNMN VNSGDGIDYS QQKRENIGDL IQETLEAFER YGGEDAFINI KYMVPTYESC LL N

UniProtKB: Parkin coregulated gene protein homolog

Macromolecule #49: Cilia- and flagella-associated protein 45

• Macromolecule: Name: Cilia- and flagella-associated protein 45 / type: protein_or_peptide / ID: 49 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 65.962016 KDa

Sequence

String:

MPLRPGDASS SASTASNRSR TRTRYRTKAM NSEVDESLFG GVKPSSQGKS DSPIVVIHDK HAIRKTLSAL GLEHKTETIQ LITRDMVRE LIVPTEDPSG ESLIISPEEF ERIKWASQVL TKEELNAREQ ALKKEKEGIL EAVTIRKKIM KQKEMTWNNN K KLSDLEEV ARERAQNLLQ RADKLRMEQE EELKDMSKII LNAKCHAIRD AQILEKQQIQ KELDEEERRL DHMMEIDRRE SL QRQEDRE RKRREERVRG KRHIVEQIKK NEEERSLQAE HREQEKEQML AYLDRLQEED LQDLERRHQE KLKMQAEIKR IND ENQRQK AEMLAQERLA DQMVMEFTKK KMAREAEYEA EQEKIRREKE KEIARLRALQ EKAQDYQAEQ DALRAKRNQE VADR EWRRK EKENAQKKIE TEEKLRKSRL EQVAFKEHTL AVQVQRDRDE FERILRAQRE QIEREKQEQE KKAKGCLQHA NELRR QVRE NQQKHVQNRL ATFEEGRRLK EEAEKRRERI EDIKKQKLEE LRATGLPEKY CIEVERKANI LPATSVN

UniProtKB: Cilia- and flagella-associated protein 45

Macromolecule #50: Sperm-associated microtubule inner protein 5

• Macromolecule: Name: Sperm-associated microtubule inner protein 5 / type: protein_or_peptide / ID: 50 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 25.847469 KDa

Sequence

String:

MESPKTFMRK LPITPGYCGF IPWLSCQESS SEDRMNPCVK AFQERTQRYK EDQQGLNCSV ANTPPLKPIC SEDTVLWVLH EYAKKYHPL TLECKNEKKP LQEPPIPGWA GYLPRARVTE FGYATRYTIM AKKCYKDFLD LVEQAKRAQL KPYEQTYDVR A AQPLSPSS KILQLQGLSP AFPEFSGPGQ TPPSEDPQAP RPCGCAQWSS QSCSRNVYGE PPSLAKAFAE S

UniProtKB: Sperm-associated microtubule inner protein 5

Macromolecule #51: Tektin-like protein 1

• Macromolecule: Name: Tektin-like protein 1 / type: protein_or_peptide / ID: 51 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 57.406484 KDa

Sequence

String:

MPVLLPSTDP GQDSRVGAPE WRQAAKATSR KAHLLTDRCG QEAVTMWQPK DSVLDPNVAH HLGRAAYMEP WRFRVEMLKG GGTLEKPPP GEGVTLWKGK MKPPAWYARL PLPMHRDARA QQTAEVVHAH ARGARLTAAR LGRAQHQING QLRLLLRQRE A TDRRLSEV RKGLLINQQS VKLRGYRPKC EKIPDKADSL LVWEKKELKS MKRKMEKDME ISEDLLKALA SCRDTLDFYC QE RLQAVEL MNQPLDKVLE QAGRHSWVDI TRPPTPRTQG LKTPPPDPIG TYTPACAKAL FEAKRLLMES KDILTEMAKN EVD IQNQQQ EISNRVCSSL AQKMRETLEL KERMTMTLGL MRGTIHRCMK FNQEMYVTRG IIKGPLLKRN LEAREKLNRP LVRM YQRHV GTQLPEATRL AQGTDLLTRH NLHMEKNLKE LRTTHDNLAW GLNCKKIGHD VDYDVVRLRL RQRHPHVCYE QAQRL VNDW DPPTPARSQT NTASK

UniProtKB: Tektin-like protein 1

Macromolecule #52: Cilia- and flagella-associated protein 52

• Macromolecule: Name: Cilia- and flagella-associated protein 52 / type: protein_or_peptide / ID: 52 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 68.322164 KDa

Sequence

String:

MEEQVLPELD VAELELQAVI GFNGHVPNGL KCHPDQEHLI YPLGCTVLIQ AINTNEQNFL HGHGNNVSCV TISKEGDYIA SGQVTFMGF KADIILWDFK KRELIARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKRDAIC GSPAAGLNVG N ATSVVFSR CRDEMFVTAG NGTIRVWELD LPNRKIWPTE CQTGQMKRIV LSTGMADDDS FFYLGTTTGD ILKMNPKTKL LA DTGPVKD KFSLGVSALR CLKMGGLLVG SGAGLLIFCK SPSYKPIKKV QLQGGITSIT LRGEGHQFFV GTEESHIYRV NFT DFKETL IATCHFEAVQ DIVFPFGTAE LFATCAKKDI RVWHTMSKRE LLRITVPNMT CHGIDFMRDG KSIISAWDDG KIRA FAPES GRLMYTINSA HRIGVTAIAT TSDCKRIISG GGEGEVRVWQ VGCQTQKLEE ALKEHKSSVS CIRVKKNNEE CVTAS TDGT CIIWDLVRLR RNQMILANTL FQCVCYHPEE FQIITSGTDR KIAYWEVFDG SVIRELEGSL SGSINGMDIT QEGGHF VTG GHDHLVKVWD YNEGEVTHVG VGHSGNIMAM RISPGNQYIV SVSADGAILR WKYPFAS

UniProtKB: Cilia- and flagella-associated protein 52

Macromolecule #53: Enkurin

• Macromolecule: Name: Enkurin / type: protein_or_peptide / ID: 53 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.587521 KDa

Sequence

String:

MDSPCTSESI YNLIPSDLKE PPQHPRYTSL FRATIKNDMK KFKTAMKTMG PAKVEIPSPK DFLKKHSKEK TLPPKKKFNR CSPKKPAVP LRTDHPVMGI QSGKNFINTN AADVIMGVAK KPKPIYVDKR TGDKHDLETS GLFPKYINKK DYGITPEYIC K RNEDVKKA QEEYDNYIQE NLKKAAMKRL SDEEREAVLQ GLKKNWEEVH KEFQSLSVFI DSVPKKIRKQ KLEKEMKQLE HD ISVIEKH KIIYIANK

UniProtKB: Enkurin

Macromolecule #54: Sperm-associated microtubule inner protein 10

• Macromolecule: Name: Sperm-associated microtubule inner protein 10 / type: protein_or_peptide / ID: 54 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.305608 KDa

Sequence

String:

MASEKDDGPA LPKLDDDNQT AENTCKPAEE QPQQLRWDDI HLPRFSLKQG MIPTRYVMPW KENMKFRNVN LQQAEACGIY AGPLEDSLF WGYSERLCHG EDRKAVLKKG LPEIKITDMP LHSPLSRYQS TVISHGFRRR LI

UniProtKB: Sperm-associated microtubule inner protein 10

Macromolecule #55: Protein Flattop

• Macromolecule: Name: Protein Flattop / type: protein_or_peptide / ID: 55 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.575123 KDa

Sequence

String:

MATNYSANQY EKAYLPTYLQ NWSPARPTKE KIAAHEGYTQ IIANDRGHLL PSVPRSKASP WGSFMGTWQM PLKIPPAKVT LTARTTTAA DNLTKWIHKN PDLLNACNGL RPEISGKPFD PDSQTKQKKS VTKTVQQAPN PTIIPSSPVI QGDNPDEPQS S HPSAGHTP GPQTPVNSPN NPPPSPCKST K

UniProtKB: Protein Flattop

Macromolecule #56: EF-hand calcium-binding domain-containing protein 6

• Macromolecule: Name: EF-hand calcium-binding domain-containing protein 6 / type: protein_or_peptide / ID: 56 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 176.028312 KDa

Sequence

String:

MKRNGTRLNF AKANSTKSGS TRARDLMNKM SIIPEWHSLY SNTRKLPCSR PHSSPCKMQR TIFQDLCRPS SSTTAIANPV LSYLDIERI LAQKISSRRD DIKKVFQILD RNHNQMVTKG DLKRVITAFL IPLTKDQFQD LLAQIPISSL GNVPYLEFLS R FGGGIDIN INGIKRVNEN EVDNRRTVKE VQLTEKIFRN MRSIRKVFQV MDVNNTGLVQ PQELRRVLET FCLRMQDGDY EK FLEQYNI DKTTAVDYNA FLKNLSVKND ASFKYLLSNA AELSRETQQG KNGKRLLDTG SSEDVWKNYS LDDLEKTFCQ EFS KSYEKI EKALSAGDPS KGGYISLNYL KVVLDTFIYR LPRRIFIQLI RRFGLKTSTK INWKQFLTAI YERQKLEVSK TLPL KKRSS TEARNRSRKE NIIKKLFKYS EDRYTALKKT LLIISSTPSG HITWEELRHI LNCMVAKLND SEFSELKQTF DPEGT GAVR VNSLLDVLDD STKVRKMSPS TDTKTPLPVA WDSVEELVLD SITRNLQAFY SMLQSYDLRD TGTIGKNNFR KVMRVF CPY LSNEHLVRFS SKFQEAGSGR ILYKKFLLSI GVGIPPPTPP LSSPKVQLSD QFQIEEPGQQ DERTQPSGEK TSEINNM TK EEVIDGLKHR IQQKDPVFRK QFLSISKEPD VKINQEEFRK VLERSGMPMN DCQYAMLASK LGFKNEEGMS YQDFTMGF E DCMLSGLETV PLQSRTASRT NMDEHFISAE ECLRIFPKKL KESFRDVYSA FFRIDLDRDG IISMHDFHRL LQYLQLNMV DLEFERFLSL LGLRLSVTLN FREFQNLCEK RPWKSDEAPQ RLIRCKQKVA DSELACEQAH QYLIMKAKTR WADLSKNFIE TDNEGNGIL RRRDIKNSLY GFDIPLTPRE FEKLWQNYDT EGRGYITYQE FLHRLGIRYS PKVHRPYKED YFNFLGHFTK P KQVQEEIQ ELQQISEREK LMNHYEEISK AFNAMEKSKP VALCRVQKVL QECGCPLKEE ELISLLKSLD VSVHNNHIDP VE FLRALEI SWASKARPKE KEESSPPPIS FSKVTPDEVI KTMQEVVESS QPALVEAFSA LDKEDTGFVK AMEFGDVLRS VCQ KLTDNQ YHYFLRRLRL HLTPNIHWKY FLENFSTFQD ETADDWAENM PKAPPPMSPK ETAHRDIVAR VQKAVASHYH TIVQ EFENF DTLKSNTVSR DEFRSICTRH IQILTDEQFD RLWSELPVNA KGRLKYQDFL SKLSIERVPS PPMAAGDSGE STMAQ RGSS APEFSQGTRS NLYSPPRDSR VGLKSRSHPC TPVGTPPLQN CEPIESRLRK QIQGCWRELL RECKEKDTDK QGTISA AEF LALVEKFKLD ISREESQQLI VKYDLKNNGK FAYCDFIQSC VLLLKAKETS LMRRMRIQNA DKMKEAGMET PSFYSAL LR IQPKIVHCWR PMRRSFKTYD KNGTGLLSVA DFRKVLRQYS INLSEEEFFH VLEYYDKSLS SKISYNDFLR AFLQ

UniProtKB: EF-hand calcium-binding domain-containing protein 6

Macromolecule #57: Cilia- and flagella- associated protein 210

• Macromolecule: Name: Cilia- and flagella- associated protein 210 / type: protein_or_peptide / ID: 57 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 65.26652 KDa

Sequence

String:

MQVRFGRRSG QAKESQGMDC FEKEEIPYPP LLPSKVDLQQ ITIIPHAEWE RIRDSLNRLT REAAVLRAER AAKKKMHVKS QELVKHWTN TYAGMKEQKL KAKQKRDEEI EAERKVLDLE EEIYKEGERK KAIESARQCQ FHQTERVKRF HSGLLLSRVM K ERDVQIQY KKNAVKSDKK WEEQVKLNDE KAFKEDQEKE EKRRRERVAL AEDHLKQIEE HKEEEEARKK SEEKDAEEMK RQ NLLYEIE MKKNLSKKQE EIDTNRKLLL DNMHNKNIIR AVEQQQQEEE DEKIRKFIKA KKRLIQMRMD KDAETHRLME ERR ERINNF LSKLIKEKLD TEDLIIARDI SEADAELEKR EKEKHEKNQA DLKAIAEYRA SVMKNKEEEE RQRKIEAKEQ LQAV LKADK IFQELEKEKS LKVTREKLEI QDAHIQQIAI NKYNAKQMKE EELDYWRLTD ALTVEKEKEF EKYAREVINF ESEST KKYA YPMVKAVQEG VGGGRGPPFV GRGGIRPSYQ ATDATGVQLP CFKSQGSKYN DFQKSKRRLG FTW

UniProtKB: Cilia- and flagella- associated protein 210

Macromolecule #58: Cilia- and flagella-associated protein 276

• Macromolecule: Name: Cilia- and flagella-associated protein 276 / type: protein_or_peptide / ID: 58 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.960092 KDa

Sequence

String:

MLQPQETFSN PALRDEDKHL GNLWASKKSL YKNPAHLAQQ QDPWSRLSST PTATSRSRDT FFDSKIPKDD LDFRLATLYN HHTGAFKNK TEILLHQETI EDIQGTKIQF PGECFHAPSA PITSRTTIRH WINPKKESIH SIQGSIVSPH TAATNGGYSR K NDGGFFST

UniProtKB: Cilia- and flagella-associated protein 276

Macromolecule #59: Piercer of microtubule wall 1 protein

• Macromolecule: Name: Piercer of microtubule wall 1 protein / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.862852 KDa

Sequence

String:

MSEEKPQQSA EEPEPGEPKA KPAPEEPEPG EPKAKPAPEE PEPGEPKAKP APEKTSDYYR ISEKLPVRFN NPGWFHGYGT KEAVSMYRT SNQTYGSRAP TVHEMPKVFY PSSNKFSRQH AAFGMFQSHN INVTLEKSLV TGPDNHITHY DRLNFHPSYN V NRPSICD

UniProtKB: Piercer of microtubule wall 1 protein

Macromolecule #60: Sperm microtubule inner protein 2

• Macromolecule: Name: Sperm microtubule inner protein 2 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.372723 KDa

Sequence

String:

MALSHREPHC ETVGKRMIVT GPDYVKDHLP KVHQHTAYIG EKRPALEKTG DLRYLWRPAS NRSLPAKYKH EYTCGIGWGI PQYSFFNRS RVESGFHIQH GELSLRAMDK ITHRYQNPWQ PKAFVLNKQL GYSRGFLAWN MSDYEDIEQR NSKRAILVKQ S KLIRSNLP SRLPKLQEKK KWKFNSQDKY F

UniProtKB: Sperm microtubule inner protein 2

Macromolecule #61: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 61 / Number of copies: 167 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #62: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 62 / Number of copies: 170 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #63: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 63 / Number of copies: 170 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #64: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 64 / Number of copies: 3 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.2
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 1.3 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION

Startup model

Type of model: EMDB MAP
EMDB ID:

35823

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 83043
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD