[English] 日本語
Yorodumi
- PDB-9v10: Cryo-EM structure of the 48-nm repeat doublet microtubule from mo... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 9v10
TitleCryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm
Components
  • (Cilia- and flagella-associated protein ...) x 13
  • (Ciliary microtubule inner protein ...) x 5
  • (EF-hand calcium-binding domain-containing protein ...) x 2
  • (EF-hand domain-containing family member ...) x 2
  • (Piercer of microtubule wall ...) x 2
  • (RIB43A-like with coiled-coils protein ...) x 2
  • (Sperm microtubule inner protein ...) x 4
  • (Sperm-associated microtubule inner protein ...) x 3
  • Cilia- and flagella- associated protein 210
  • Ciliary microtubule associated protein 1A
  • Dual specificity phosphatase 21
  • EF-hand domain-containing protein 1
  • Enkurin
  • Luteinizing hormone beta
  • Meiosis-specific nuclear structural protein 1
  • Nucleoside diphosphate kinase homolog 7
  • Parkin coregulated gene protein homolog
  • Protein Flattop
  • Protein SPMIP1
  • Protein SPMIP3
  • Protein SPMIP7
  • Protein SPMIP9
  • Sperm axonemal maintenance protein CFAP97D1
  • Sperm-associated antigen 8
  • Stabilizer of axonemal microtubules 4
  • Tektin bundle-interacting protein 1
  • Tektin-1
  • Tektin-2
  • Tektin-3
  • Tektin-4
  • Tektin-5
  • Tektin-like protein 1
  • Testis-specific serine/threonine-protein kinase 6
  • Tubulin alpha-3 chain
  • Tubulin beta-4B chain
KeywordsSTRUCTURAL PROTEIN / sperm flagellar doublet microtubules
Function / homology
Function and homology information


protein localization to motile cilium / axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath ...protein localization to motile cilium / axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / outer dense fiber / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / protein polyglutamylation / left/right pattern formation / regulation of calcineurin-NFAT signaling cascade / sperm axoneme assembly / regulation of microtubule nucleation / Carboxyterminal post-translational modifications of tubulin / Intraflagellar transport / positive regulation of feeding behavior / COPI-independent Golgi-to-ER retrograde traffic / MAP kinase tyrosine/serine/threonine phosphatase activity / inner dynein arm assembly / cilium-dependent cell motility / cerebrospinal fluid circulation / regulation of cilium beat frequency involved in ciliary motility / epithelial cilium movement involved in extracellular fluid movement / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / 9+2 motile cilium / COPI-mediated anterograde transport / cilium movement involved in cell motility / intraciliary transport / regulation of store-operated calcium entry / Kinesins / acrosomal membrane / axoneme assembly / PKR-mediated signaling / cilium movement / left/right axis specification / Aggrephagy / RHO GTPases activate IQGAPs / microtubule sliding / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / Recycling pathway of L1 / calcium ion sensor activity / The role of GTSE1 in G2/M progression after G2 checkpoint / sperm DNA condensation / COPI-dependent Golgi-to-ER retrograde traffic / axonemal microtubule / Hedgehog 'off' state / RHO GTPases Activate Formins / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / Separation of Sister Chromatids / cilium organization / Recruitment of NuMA to mitotic centrosomes / AURKA Activation by TPX2 / gamma-tubulin ring complex / manchette / Regulation of PLK1 Activity at G2/M Transition / positive regulation of cilium assembly / MHC class II antigen presentation / flagellated sperm motility / 3'-5'-DNA exonuclease activity / UTP biosynthetic process / CTP biosynthetic process / motile cilium / determination of left/right symmetry / DNA catabolic process / extrinsic component of membrane / nucleoside diphosphate kinase activity / protein targeting to membrane / : / positive regulation of cell motility / GTP biosynthetic process / intermediate filament / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / tubulin complex / protein-serine/threonine phosphatase / AMP binding / ciliary base / regulation of neuron projection development / protein serine/threonine phosphatase activity / receptor clustering / cerebral cortex cell migration / ciliary transition zone / phosphatase activity / mitotic cytokinesis / microtubule organizing center / cellular response to UV-C / cilium assembly / cellular response to unfolded protein / regulation of cell division
Similarity search - Function
Spermatogenesis-associated protein 48 / Protein of unknown function DUF4555 / Spermatogenesis-associated protein 48 / Sperm-associated microtubule inner protein 4 / Uncharacterized protein C1orf100 / : / Family of unknown function (DUF5530) / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 ...Spermatogenesis-associated protein 48 / Protein of unknown function DUF4555 / Spermatogenesis-associated protein 48 / Sperm-associated microtubule inner protein 4 / Uncharacterized protein C1orf100 / : / Family of unknown function (DUF5530) / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 / Testis-expressed protein 33 / Testis-expressed sequence 37 protein / CFAP97 domain-containing protein 1/2 / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / : / : / Normal lung function maintenance, Low in Lung Cancer 1 protein / Domain of unknown function (DUF4562) / Spermatid-specific manchette-related protein 1 / TSC21 family / Testis-expressed sequence 33 protein family / Sperm-associated microtubule inner protein 5 / Ciliary microtubule inner protein 3 / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Sperm microtubule inner protein 8 / Sperm microtubule inner protein 11 / : / : / Sperm microtubule inner protein 1 / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Cilia- and flagella-associated protein 68 / Sperm-associated microtubule inner protein 10 / Protein of unknown function DUF4541 / Tektin bundle interacting protein 1 / Hemingway/CFA97 / Coiled-coil domain-containing protein 105 / : / Hemingway/CFA97 / Tektin bundle interacting protein 1 / Cilia and flagella associated protein 90 / : / DJBP, EF-hand domain / FAM183 family / EF-hand domain / FAM183A and FAM183B related / Sperm-tail PG-rich repeat / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Sperm-tail PG-rich repeat / Protein CFAP95 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / : / Tektin family / Protein of unknown function (DUF3695) / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 161-like domain / : / : / EFHB C-terminal EF-hand domain / : / : / Nucleoside diphosphate kinase 7 N-terminal PH domain / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / :
Similarity search - Domain/homology
ADENOSINE-5'-TRIPHOSPHATE / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Cilia- and flagella-associated protein 77 / Luteinizing hormone beta / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Protein SPMIP1 / Sperm microtubule inner protein 2 / Tektin-5 ...ADENOSINE-5'-TRIPHOSPHATE / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Cilia- and flagella-associated protein 77 / Luteinizing hormone beta / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Protein SPMIP1 / Sperm microtubule inner protein 2 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Stabilizer of axonemal microtubules 4 / Tektin-4 / Sperm microtubule inner protein 6 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 107 / Piercer of microtubule wall 1 protein / Cilia- and flagella-associated protein 52 / Cilia- and flagella-associated protein 144 / Protein SPMIP7 / Meiosis-specific nuclear structural protein 1 / Sperm microtubule inner protein 8 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Cilia- and flagella-associated protein 161 / Enkurin / Tektin-3 / EF-hand calcium-binding domain-containing protein 3 / Cilia- and flagella-associated protein 20 / Sperm microtubule inner protein 11 / EF-hand domain-containing family member B / Ciliary microtubule inner protein 3 / Ciliary microtubule associated protein 1A / Tektin-2 / Testis-specific serine/threonine-protein kinase 6 / Cilia- and flagella-associated protein 95 / Sperm-associated microtubule inner protein 5 / RIB43A-like with coiled-coils protein 1 / Cilia- and flagella-associated protein 68 / Cilia- and flagella-associated protein 53 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 2A / Tektin-like protein 1 / RIB43A-like with coiled-coils protein 2 / Sperm-associated microtubule inner protein 4 / Dual specificity phosphatase 21 / Cilia- and flagella-associated protein 141 / Sperm-associated microtubule inner protein 10 / Ciliary microtubule inner protein 4 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 45 / Ciliary microtubule inner protein 1 / Protein SPMIP3 / Cilia- and flagella-associated protein 276 / Protein SPMIP9 / Tektin-1 / Parkin coregulated gene protein homolog / Sperm axonemal maintenance protein CFAP97D1 / Cilia- and flagella-associated protein 90 / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 2 protein
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsLiu, Q. / Gui, M. / Wu, J.P. / Zhou, L.N.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Nat Commun / Year: 2026
Title: Doublet microtubule-associated tektins and enzymes differentially regulate sperm flagellar integrity and motility.
Authors: Qi Liu / Lunni Zhou / Xiaochen Liang / Pengyu Chen / Bo Li / Shuo Yang / Yuqi Liu / Haibo Zhao / Jin Hu / Shan Feng / Shanshan Xie / Jianping Wu / Miao Gui /
Abstract: Doublet microtubule (DMT)-associated proteins assemble and drive sperm flagella, which are essential for successful fertilization. However, the exact roles of different DMT-associated proteins in ...Doublet microtubule (DMT)-associated proteins assemble and drive sperm flagella, which are essential for successful fertilization. However, the exact roles of different DMT-associated proteins in sperm function and the underlying molecular mechanisms remain elusive. Here, we generate four gene-knockout mice based on high-resolution structures targeting distinct DMT components: two intermediate filament-like tektins (TEKT1, TEKT5) and two enzymes (TSSK6, DUSP21). The depletion of TEKT1, shared by sperm flagella and motile cilia, causes male infertility characterized by impaired sperm motility and loss of the tektin bundle, whereas sperm-specific Tekt5 knockout (KO) mice remain fertile with largely normal flagellar function, indicating functional divergence within the tektin family. Tssk6 KO spermatozoa exhibit severely disturbed morphology and motility, resulting in homozygote infertility and heterozygote subfertility. Phosphoproteomics reveals dysregulated phosphorylation of axonemal proteins, highlighting the critical role of kinase-mediated signaling in regulating sperm motility. Conversely, Dusp21 KO mice display no fertility or sperm motility defects, suggesting compensatory phosphatase activity. Phenotypic comparisons between Tekt1 and Tssk6 KO mice suggest their involvement in distinct subtypes of asthenozoospermia. Overall, this study elucidates how filamentous and enzymatic DMT proteins govern sperm function through divergent mechanisms, which have implications for molecular diagnosis of male infertility.
History
DepositionMay 19, 2025Deposition site: PDBJ / Processing site: PDBC
Revision 1.0May 27, 2026Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
0: Cilia- and flagella-associated protein 95
1: EF-hand domain-containing family member B
2: EF-hand domain-containing family member B
3: Cilia- and flagella-associated protein 53
4: Cilia- and flagella-associated protein 53
5: Nucleoside diphosphate kinase homolog 7
6: Nucleoside diphosphate kinase homolog 7
7: Cilia- and flagella-associated protein 95
8: Cilia- and flagella-associated protein 107
A: Cilia- and flagella-associated protein 141
A0: Tektin-1
A1: Tektin-1
A2: Tektin-1
A3: Tektin-1
A4: Tektin-1
A5: Tektin-1
AA: Tubulin alpha-3 chain
AB: Tubulin beta-4B chain
AC: Tubulin alpha-3 chain
AD: Tubulin beta-4B chain
AE: Tubulin alpha-3 chain
AF: Tubulin beta-4B chain
AG: Tubulin alpha-3 chain
AH: Tubulin beta-4B chain
AI: Tubulin alpha-3 chain
AJ: Tubulin beta-4B chain
AK: Tubulin alpha-3 chain
AL: Tubulin beta-4B chain
AM: Tubulin alpha-3 chain
AN: Tubulin beta-4B chain
AO: Tubulin alpha-3 chain
AP: Tubulin beta-4B chain
Aa: Sperm axonemal maintenance protein CFAP97D1
Ab: Sperm axonemal maintenance protein CFAP97D1
Ac: Sperm axonemal maintenance protein CFAP97D1
B: Meiosis-specific nuclear structural protein 1
B0: Tektin-2
B1: Tektin-2
B2: Tektin-2
B3: Tektin-2
B4: Tektin-2
B5: Tektin-2
BB: Tubulin beta-4B chain
BC: Tubulin alpha-3 chain
BD: Tubulin beta-4B chain
BE: Tubulin alpha-3 chain
BF: Tubulin beta-4B chain
BG: Tubulin alpha-3 chain
BH: Tubulin beta-4B chain
BI: Tubulin alpha-3 chain
BJ: Tubulin beta-4B chain
BK: Tubulin alpha-3 chain
BL: Tubulin beta-4B chain
BM: Tubulin alpha-3 chain
BN: Tubulin beta-4B chain
BO: Tubulin alpha-3 chain
BP: Tubulin beta-4B chain
Ba: EF-hand calcium-binding domain-containing protein 3
Bb: EF-hand calcium-binding domain-containing protein 3
Bc: EF-hand calcium-binding domain-containing protein 3
C: Meiosis-specific nuclear structural protein 1
C0: Tektin-3
C1: Tektin-3
C2: Tektin-3
C3: Tektin-3
C4: Tektin-3
C6: Tektin-3
CC: Tubulin alpha-3 chain
CD: Tubulin beta-4B chain
CE: Tubulin alpha-3 chain
CF: Tubulin beta-4B chain
CG: Tubulin alpha-3 chain
CH: Tubulin beta-4B chain
CI: Tubulin alpha-3 chain
CJ: Tubulin beta-4B chain
CK: Tubulin alpha-3 chain
CL: Tubulin beta-4B chain
CM: Tubulin alpha-3 chain
CN: Tubulin beta-4B chain
CO: Tubulin alpha-3 chain
CP: Tubulin beta-4B chain
D: Sperm-associated antigen 8
D0: Tektin-4
D1: Tektin-4
D2: Tektin-4
D3: Tektin-4
D4: Tektin-4
D5: Tektin-4
DC: Tubulin alpha-3 chain
DD: Tubulin beta-4B chain
DE: Tubulin alpha-3 chain
DF: Tubulin beta-4B chain
DG: Tubulin alpha-3 chain
DH: Tubulin beta-4B chain
DI: Tubulin alpha-3 chain
DJ: Tubulin beta-4B chain
DK: Tubulin alpha-3 chain
DL: Tubulin beta-4B chain
DM: Tubulin alpha-3 chain
DN: Tubulin beta-4B chain
DO: Tubulin alpha-3 chain
DP: Tubulin beta-4B chain
E: Cilia- and flagella-associated protein 161
EA: Tubulin alpha-3 chain
EB: Tubulin beta-4B chain
EC: Tubulin alpha-3 chain
ED: Tubulin beta-4B chain
EE: Tubulin alpha-3 chain
EF: Tubulin beta-4B chain
EG: Tubulin alpha-3 chain
EH: Tubulin beta-4B chain
EI: Tubulin alpha-3 chain
EJ: Tubulin beta-4B chain
EK: Tubulin alpha-3 chain
EL: Tubulin beta-4B chain
EM: Tubulin alpha-3 chain
EN: Tubulin beta-4B chain
Ea: Ciliary microtubule inner protein 1
F: Cilia- and flagella-associated protein 161
FB: Tubulin beta-4B chain
FC: Tubulin alpha-3 chain
FD: Tubulin beta-4B chain
FE: Tubulin alpha-3 chain
FF: Tubulin beta-4B chain
FG: Tubulin alpha-3 chain
FH: Tubulin beta-4B chain
FI: Tubulin alpha-3 chain
FJ: Tubulin beta-4B chain
FK: Tubulin alpha-3 chain
FL: Tubulin beta-4B chain
FM: Tubulin alpha-3 chain
FN: Tubulin beta-4B chain
Fa: Ciliary microtubule inner protein 3
Fb: Ciliary microtubule inner protein 3
Fc: Ciliary microtubule inner protein 3
Fd: Ciliary microtubule inner protein 3
G: Piercer of microtubule wall 2 protein
GB: Tubulin beta-4B chain
GC: Tubulin alpha-3 chain
GD: Tubulin beta-4B chain
GE: Tubulin alpha-3 chain
GF: Tubulin beta-4B chain
GG: Tubulin alpha-3 chain
GH: Tubulin beta-4B chain
GI: Tubulin alpha-3 chain
GJ: Tubulin beta-4B chain
GK: Tubulin alpha-3 chain
GL: Tubulin beta-4B chain
GM: Tubulin alpha-3 chain
GN: Tubulin beta-4B chain
GO: Tubulin alpha-3 chain
Ga: Ciliary microtubule inner protein 4
H: Ciliary microtubule inner protein 2A
HB: Tubulin beta-4B chain
HC: Tubulin alpha-3 chain
HD: Tubulin beta-4B chain
HE: Tubulin alpha-3 chain
HF: Tubulin beta-4B chain
HG: Tubulin alpha-3 chain
HH: Tubulin beta-4B chain
HI: Tubulin alpha-3 chain
HJ: Tubulin beta-4B chain
HK: Tubulin alpha-3 chain
HL: Tubulin beta-4B chain
HM: Tubulin alpha-3 chain
HN: Tubulin beta-4B chain
HO: Tubulin alpha-3 chain
Ha: Luteinizing hormone beta
Hb: Luteinizing hormone beta
I: Ciliary microtubule inner protein 2A
I1: Cilia- and flagella-associated protein 68
IB: Tubulin beta-4B chain
IC: Tubulin alpha-3 chain
ID: Tubulin beta-4B chain
IE: Tubulin alpha-3 chain
IF: Tubulin beta-4B chain
IG: Tubulin alpha-3 chain
IH: Tubulin beta-4B chain
II: Tubulin alpha-3 chain
IJ: Tubulin beta-4B chain
IK: Tubulin alpha-3 chain
IL: Tubulin beta-4B chain
IM: Tubulin alpha-3 chain
IN: Tubulin beta-4B chain
IO: Tubulin alpha-3 chain
IP: Tubulin beta-4B chain
Ia: Protein SPMIP1
Ib: Protein SPMIP1
Ic: Protein SPMIP1
J: Ciliary microtubule inner protein 2A
J1: Cilia- and flagella-associated protein 77
J2: Cilia- and flagella-associated protein 77
J3: Cilia- and flagella-associated protein 77
J4: Cilia- and flagella-associated protein 77
J5: Cilia- and flagella-associated protein 77
JC: Tubulin alpha-3 chain
JD: Tubulin beta-4B chain
JE: Tubulin alpha-3 chain
JF: Tubulin beta-4B chain
JG: Tubulin alpha-3 chain
JH: Tubulin beta-4B chain
JI: Tubulin alpha-3 chain
JJ: Tubulin beta-4B chain
JK: Tubulin alpha-3 chain
JL: Tubulin beta-4B chain
JM: Tubulin alpha-3 chain
JN: Tubulin beta-4B chain
JO: Tubulin alpha-3 chain
JP: Tubulin beta-4B chain
JQ: Tubulin alpha-3 chain
K: Ciliary microtubule inner protein 2A
K1: Cilia- and flagella-associated protein 144
K2: Cilia- and flagella-associated protein 144
K3: Sperm-associated antigen 8
KA: Tubulin alpha-3 chain
KB: Tubulin beta-4B chain
KC: Tubulin alpha-3 chain
KD: Tubulin beta-4B chain
KE: Tubulin alpha-3 chain
KF: Tubulin beta-4B chain
KG: Tubulin alpha-3 chain
KH: Tubulin beta-4B chain
KI: Tubulin alpha-3 chain
KJ: Tubulin beta-4B chain
KK: Tubulin alpha-3 chain
KL: Tubulin beta-4B chain
KM: Tubulin alpha-3 chain
KN: Tubulin beta-4B chain
KO: Tubulin alpha-3 chain
KP: Tubulin beta-4B chain
Ka: Protein SPMIP3
Kb: Protein SPMIP3
L: Ciliary microtubule inner protein 2A
L1: Cilia- and flagella-associated protein 90
LA: Tubulin alpha-3 chain
LB: Tubulin beta-4B chain
LC: Tubulin alpha-3 chain
LD: Tubulin beta-4B chain
LE: Tubulin alpha-3 chain
LF: Tubulin beta-4B chain
LG: Tubulin alpha-3 chain
LH: Tubulin beta-4B chain
LI: Tubulin alpha-3 chain
LJ: Tubulin beta-4B chain
LK: Tubulin alpha-3 chain
LL: Tubulin beta-4B chain
LM: Tubulin alpha-3 chain
LN: Tubulin beta-4B chain
LO: Tubulin alpha-3 chain
LP: Tubulin beta-4B chain
La: Sperm-associated microtubule inner protein 4
Lb: Sperm-associated microtubule inner protein 4
M: Ciliary microtubule inner protein 2A
M1: Ciliary microtubule inner protein 2C
M2: Ciliary microtubule inner protein 2C
M3: Ciliary microtubule inner protein 2C
M4: Ciliary microtubule inner protein 2C
MA: Tubulin alpha-3 chain
MB: Tubulin beta-4B chain
MC: Tubulin alpha-3 chain
MD: Tubulin beta-4B chain
ME: Tubulin alpha-3 chain
MF: Tubulin beta-4B chain
MG: Tubulin alpha-3 chain
MH: Tubulin beta-4B chain
MI: Tubulin alpha-3 chain
MJ: Tubulin beta-4B chain
MK: Tubulin alpha-3 chain
ML: Tubulin beta-4B chain
MM: Tubulin alpha-3 chain
MN: Tubulin beta-4B chain
MO: Tubulin alpha-3 chain
MP: Tubulin beta-4B chain
Ma: Protein SPMIP7
N: Ciliary microtubule inner protein 2A
N1: Cilia- and flagella-associated protein 141
N2: Cilia- and flagella-associated protein 161
NA: Tubulin alpha-3 chain
NB: Tubulin beta-4B chain
NC: Tubulin alpha-3 chain
ND: Tubulin beta-4B chain
NE: Tubulin alpha-3 chain
NF: Tubulin beta-4B chain
NG: Tubulin alpha-3 chain
NH: Tubulin beta-4B chain
NI: Tubulin alpha-3 chain
NJ: Tubulin beta-4B chain
NK: Tubulin alpha-3 chain
NL: Tubulin beta-4B chain
NM: Tubulin alpha-3 chain
NN: Tubulin beta-4B chain
NO: Tubulin alpha-3 chain
NP: Tubulin beta-4B chain
Na: Sperm microtubule inner protein 8
Nb: Sperm microtubule inner protein 8
Nc: Sperm microtubule inner protein 8
Nd: Sperm microtubule inner protein 8
Ne: Sperm microtubule inner protein 8
O: RIB43A-like with coiled-coils protein 1
O1: Dual specificity phosphatase 21
O2: Dual specificity phosphatase 21
O3: Dual specificity phosphatase 21
O4: Dual specificity phosphatase 21
O5: EF-hand domain-containing protein 1
O6: EF-hand domain-containing protein 1
O7: Ciliary microtubule inner protein 2A
O8: Ciliary microtubule inner protein 2A
O9: Ciliary microtubule inner protein 2A
OB: Tubulin beta-4B chain
OC: Tubulin alpha-3 chain
OD: Tubulin beta-4B chain
OE: Tubulin alpha-3 chain
OF: Tubulin beta-4B chain
OG: Tubulin alpha-3 chain
OH: Tubulin beta-4B chain
OI: Tubulin alpha-3 chain
OJ: Tubulin beta-4B chain
OK: Tubulin alpha-3 chain
OL: Tubulin beta-4B chain
OM: Tubulin alpha-3 chain
ON: Tubulin beta-4B chain
OO: Tubulin alpha-3 chain
OP: Tubulin beta-4B chain
Oa: Sperm microtubule inner protein 6
Ob: Sperm microtubule inner protein 6
Oc: Sperm microtubule inner protein 6
Od: Sperm microtubule inner protein 6
Oe: Sperm microtubule inner protein 6
Of: Sperm microtubule inner protein 6
Og: Sperm microtubule inner protein 6
Oh: Sperm microtubule inner protein 6
P: RIB43A-like with coiled-coils protein 1
P1: Sperm microtubule inner protein 11
P2: Sperm microtubule inner protein 11
P3: Protein SPMIP9
P4: Protein SPMIP9
P5: Protein SPMIP9
P6: Protein SPMIP9
P7: Protein SPMIP9
P8: Ciliary microtubule inner protein 2A
P9: Ciliary microtubule inner protein 2A
PB: Tubulin beta-4B chain
PC: Tubulin alpha-3 chain
PD: Tubulin beta-4B chain
PE: Tubulin alpha-3 chain
PF: Tubulin beta-4B chain
PG: Tubulin alpha-3 chain
PH: Tubulin beta-4B chain
PI: Tubulin alpha-3 chain
PJ: Tubulin beta-4B chain
PK: Tubulin alpha-3 chain
PL: Tubulin beta-4B chain
PM: Tubulin alpha-3 chain
PN: Tubulin beta-4B chain
PO: Tubulin alpha-3 chain
PP: Tubulin beta-4B chain
Q: RIB43A-like with coiled-coils protein 1
Q1: Tektin bundle-interacting protein 1
Q2: Tektin bundle-interacting protein 1
Q3: Tektin bundle-interacting protein 1
Q4: Tektin bundle-interacting protein 1
QC: Tubulin alpha-3 chain
QD: Tubulin beta-4B chain
QE: Tubulin alpha-3 chain
QF: Tubulin beta-4B chain
QG: Tubulin alpha-3 chain
QH: Tubulin beta-4B chain
QI: Tubulin alpha-3 chain
QJ: Tubulin beta-4B chain
QK: Tubulin alpha-3 chain
QL: Tubulin beta-4B chain
QM: Tubulin alpha-3 chain
QN: Tubulin beta-4B chain
QO: Tubulin alpha-3 chain
QP: Tubulin beta-4B chain
R: RIB43A-like with coiled-coils protein 2
R0: Tektin-2
R1: Tektin-2
R2: Tektin-2
R3: Tektin-2
R4: Tektin-2
R5: Tektin-2
RC: Tubulin alpha-3 chain
RD: Tubulin beta-4B chain
RE: Tubulin alpha-3 chain
RF: Tubulin beta-4B chain
RG: Tubulin alpha-3 chain
RH: Tubulin beta-4B chain
RI: Tubulin alpha-3 chain
RJ: Tubulin beta-4B chain
RK: Tubulin alpha-3 chain
RL: Tubulin beta-4B chain
RM: Tubulin alpha-3 chain
RN: Tubulin beta-4B chain
RO: Tubulin alpha-3 chain
RP: Tubulin beta-4B chain
S: RIB43A-like with coiled-coils protein 2
S0: Tektin-3
S1: Tektin-3
S2: Tektin-3
S3: Tektin-3
S4: Tektin-3
S5: Tektin-3
S6: Tektin-3
S7: Tektin-3
S8: Tektin-3
S9: Tektin-3
SA: Tubulin alpha-3 chain
SB: Tubulin beta-4B chain
SC: Tubulin alpha-3 chain
SD: Tubulin beta-4B chain
SE: Tubulin alpha-3 chain
SF: Tubulin beta-4B chain
SG: Tubulin alpha-3 chain
SH: Tubulin beta-4B chain
SI: Tubulin alpha-3 chain
SJ: Tubulin beta-4B chain
SK: Tubulin alpha-3 chain
SL: Tubulin beta-4B chain
SM: Tubulin alpha-3 chain
SN: Tubulin beta-4B chain
T: EF-hand domain-containing protein 1
T0: Tektin-4
T1: Tektin-4
T2: Tektin-4
T3: Tektin-4
T4: Tektin-4
T5: Tektin-4
TA: Tubulin alpha-3 chain
TB: Tubulin beta-4B chain
TC: Tubulin alpha-3 chain
TD: Tubulin beta-4B chain
TE: Tubulin alpha-3 chain
TF: Tubulin beta-4B chain
TG: Tubulin alpha-3 chain
TH: Tubulin beta-4B chain
TI: Tubulin alpha-3 chain
TJ: Tubulin beta-4B chain
TK: Tubulin alpha-3 chain
TL: Tubulin beta-4B chain
TM: Tubulin alpha-3 chain
TN: Tubulin beta-4B chain
U: EF-hand domain-containing protein 1
U0: Tektin-5
U1: Tektin-5
U2: Tektin-5
U3: Tektin-5
U4: Tektin-5
U5: Tektin-5
U6: Tektin-5
U7: Tektin-5
U8: Tektin-5
U9: Tektin-5
UA: Tubulin alpha-3 chain
UB: Tubulin beta-4B chain
UC: Tubulin alpha-3 chain
UD: Tubulin beta-4B chain
UE: Tubulin alpha-3 chain
UF: Tubulin beta-4B chain
UG: Tubulin alpha-3 chain
UH: Tubulin beta-4B chain
UI: Tubulin alpha-3 chain
UJ: Tubulin beta-4B chain
UK: Tubulin alpha-3 chain
UL: Tubulin beta-4B chain
UM: Tubulin alpha-3 chain
UN: Tubulin beta-4B chain
V: EF-hand domain-containing protein 1
V0: Tektin-5
V1: Tektin-5
V2: Tektin-5
V3: Tektin-5
V4: Tektin-5
V5: Tektin-5
V6: Tektin-5
V7: Tektin-5
V8: Tektin-5
V9: Tektin-5
VA: Tubulin alpha-3 chain
VB: Tubulin beta-4B chain
VC: Tubulin alpha-3 chain
VD: Tubulin beta-4B chain
VE: Tubulin alpha-3 chain
VF: Tubulin beta-4B chain
VG: Tubulin alpha-3 chain
VH: Tubulin beta-4B chain
VI: Tubulin alpha-3 chain
VJ: Tubulin beta-4B chain
VK: Tubulin alpha-3 chain
VL: Tubulin beta-4B chain
VM: Tubulin alpha-3 chain
VN: Tubulin beta-4B chain
VO: Tubulin alpha-3 chain
W: EF-hand domain-containing family member C2
W0: Tektin-5
W2: Tektin-5
W4: Tektin-5
W5: Tektin-5
W6: Tektin-5
W7: Tektin-5
WB: Tubulin beta-4B chain
WC: Tubulin alpha-3 chain
WD: Tubulin beta-4B chain
WE: Tubulin alpha-3 chain
WF: Tubulin beta-4B chain
WG: Tubulin alpha-3 chain
WH: Tubulin beta-4B chain
WI: Tubulin alpha-3 chain
WJ: Tubulin beta-4B chain
WK: Tubulin alpha-3 chain
WL: Tubulin beta-4B chain
WM: Tubulin alpha-3 chain
WN: Tubulin beta-4B chain
WO: Tubulin alpha-3 chain
X: EF-hand domain-containing family member C2
X4: Stabilizer of axonemal microtubules 4
X5: Stabilizer of axonemal microtubules 4
X6: Testis-specific serine/threonine-protein kinase 6
X7: Testis-specific serine/threonine-protein kinase 6
X8: Testis-specific serine/threonine-protein kinase 6
XB: Cilia- and flagella-associated protein 20
XC: Cilia- and flagella-associated protein 20
XD: Cilia- and flagella-associated protein 20
XE: Cilia- and flagella-associated protein 20
XF: Cilia- and flagella-associated protein 20
XG: Cilia- and flagella-associated protein 20
XH: Cilia- and flagella-associated protein 20
Y: EF-hand domain-containing family member C2
Y0: Ciliary microtubule associated protein 1A
Y1: Ciliary microtubule associated protein 1A
Y2: Ciliary microtubule associated protein 1A
Y3: Ciliary microtubule associated protein 1A
Y4: Ciliary microtubule associated protein 1A
Y5: Ciliary microtubule associated protein 1A
Y6: Ciliary microtubule associated protein 1A
Y7: Ciliary microtubule associated protein 1A
YA: Parkin coregulated gene protein homolog
YB: Parkin coregulated gene protein homolog
YC: Parkin coregulated gene protein homolog
YD: Parkin coregulated gene protein homolog
YE: Parkin coregulated gene protein homolog
YF: Parkin coregulated gene protein homolog
YG: Parkin coregulated gene protein homolog
YH: Parkin coregulated gene protein homolog
Z: EF-hand domain-containing family member C2
Z2: Ciliary microtubule inner protein 2A
Z3: Ciliary microtubule inner protein 2A
Z4: Ciliary microtubule inner protein 2A
Z5: Ciliary microtubule inner protein 2A
a: Cilia- and flagella-associated protein 45
a1: Sperm-associated microtubule inner protein 5
a2: Sperm-associated microtubule inner protein 5
a3: Sperm-associated microtubule inner protein 5
a4: Sperm-associated microtubule inner protein 5
a6: Cilia- and flagella-associated protein 45
b: Cilia- and flagella-associated protein 45
b1: Tektin-like protein 1
b2: Tektin-like protein 1
b3: Tektin-like protein 1
b4: Tektin-like protein 1
b5: Tektin-like protein 1
c: Cilia- and flagella-associated protein 45
d: Cilia- and flagella-associated protein 45
e: Cilia- and flagella-associated protein 52
f: Cilia- and flagella-associated protein 52
g: Cilia- and flagella-associated protein 52
h: Enkurin
h1: Sperm-associated microtubule inner protein 10
h2: Sperm-associated microtubule inner protein 10
h3: Sperm-associated microtubule inner protein 10
h4: Sperm-associated microtubule inner protein 10
i: Enkurin
i1: Protein Flattop
i2: EF-hand calcium-binding domain-containing protein 6
i3: EF-hand calcium-binding domain-containing protein 6
i4: EF-hand calcium-binding domain-containing protein 6
j: Enkurin
j1: Nucleoside diphosphate kinase homolog 7
k: Enkurin
l: Protein Flattop
m: Protein Flattop
n: Protein Flattop
o: Cilia- and flagella- associated protein 210
p: Cilia- and flagella- associated protein 210
q: Cilia- and flagella-associated protein 276
r: Cilia- and flagella-associated protein 276
s: Cilia- and flagella-associated protein 276
y: Piercer of microtubule wall 1 protein
ke: Sperm microtubule inner protein 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,941,6681099
Polymers27,773,299589
Non-polymers168,369510
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

-
Components

+
Cilia- and flagella-associated protein ... , 13 types, 37 molecules 07348AN1EFN2I1J1J2J3J4J5K1K2L1XBXCXDXEXFXGXHaa6bc...

#1: Protein Cilia- and flagella-associated protein 95


Mass: 26633.035 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CQC3
#3: Protein Cilia- and flagella-associated protein 53 / Coiled-coil domain-containing protein 11


Mass: 62036.609 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D439
#5: Protein Cilia- and flagella-associated protein 107


Mass: 23062.510 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q4KKZ1
#6: Protein Cilia- and flagella-associated protein 141


Mass: 12278.145 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9D9
#17: Protein Cilia- and flagella-associated protein 161


Mass: 34433.383 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6P8Y0
#24: Protein Cilia- and flagella-associated protein 68


Mass: 19552.668 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D131
#26: Protein
Cilia- and flagella-associated protein 77


Mass: 32786.020 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A0A087WRI3
#27: Protein Cilia- and flagella-associated protein 144 / Testis highly expressed protein 6


Mass: 16155.242 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q5NC57
#29: Protein Cilia- and flagella-associated protein 90 / Y regulated sperm protein


Mass: 20886.428 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAR0
#46: Protein
Cilia- and flagella-associated protein 20 / Gene trap locus 3 protein


Mass: 22781.389 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8BTU1
#49: Protein
Cilia- and flagella-associated protein 45 / Coiled-coil domain-containing protein 19 / Nasopharyngeal epithelium-specific protein 1


Mass: 65962.016 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9U9
#52: Protein Cilia- and flagella-associated protein 52 / WD repeat-containing protein 16


Mass: 68322.164 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q5F201
#58: Protein Cilia- and flagella-associated protein 276


Mass: 18960.092 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAD0

+
EF-hand domain-containing family member ... , 2 types, 6 molecules 12WXYZ

#2: Protein EF-hand domain-containing family member B / Cilia- and flagella-associated protein 21


Mass: 95891.961 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8CDU5
#43: Protein
EF-hand domain-containing family member C2


Mass: 87758.023 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D485

+
Protein , 27 types, 481 molecules 56j1A0A1A2A3A4A5AAACAEAGAIAKAMAOBCBEBGBIBKBMBOCCCECGCICKCM...

#4: Protein Nucleoside diphosphate kinase homolog 7 / NDK 7 / NDP kinase homolog 7 / 3'-5' exonuclease NME7 / Protein kinase NME7 / nm23-H7


Mass: 44489.828 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: Q9QXL8, Hydrolases; Acting on ester bonds, Transferases; Transferring phosphorus-containing groups
#7: Protein
Tektin-1


Mass: 48713.035 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAJ2
#8: Protein ...
Tubulin alpha-3 chain / Alpha-tubulin 3/7 / Alpha-tubulin isotype M-alpha-3/7 / Tubulin alpha-3/alpha-7 chain


Mass: 50012.359 Da / Num. of mol.: 167 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: P05214, Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
#9: Protein ...
Tubulin beta-4B chain / Tubulin beta-2C chain


Mass: 49877.824 Da / Num. of mol.: 170 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: P68372
#10: Protein Sperm axonemal maintenance protein CFAP97D1 / CFAP97 domain-containing protein 1


Mass: 19811.641 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAN9
#11: Protein Meiosis-specific nuclear structural protein 1


Mass: 60353.012 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q61884
#12: Protein
Tektin-2 / Tektin-t / Testicular tektin


Mass: 50385.066 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q922G7
#14: Protein
Tektin-3


Mass: 56754.777 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6X6Z7
#15: Protein Sperm-associated antigen 8 / Sperm membrane protein 1 / SMP-1 / Sperm membrane protein BS-84


Mass: 50591.805 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q3V0Q6
#16: Protein
Tektin-4 / Testicular microtubules-related protein 4


Mass: 52121.449 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q149S1
#23: Protein Luteinizing hormone beta


Mass: 34424.383 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A0A571BEL8
#25: Protein Protein SPMIP1 / ATP6V1F neighbor gene protein homolog / Protein ATP6V1FNB / Sperm-associated microtubule inner protein 1


Mass: 20439.783 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: B9EJX3
#28: Protein Protein SPMIP3 / Sperm microtubule inner protein 3 / Sperm-associated microtubule inner protein 3


Mass: 17001.305 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAA7
#32: Protein Protein SPMIP7 / Post-meiotic spermatogenesis protein 1 / Sperm microtubule inner protein 7 / Spermatogenesis- ...Post-meiotic spermatogenesis protein 1 / Sperm microtubule inner protein 7 / Spermatogenesis-associated protein 48


Mass: 50476.312 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q5NC83
#35: Protein
Dual specificity phosphatase 21


Mass: 21527.574 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: Q9D9D8, protein-serine/threonine phosphatase, protein-tyrosine-phosphatase
#36: Protein
EF-hand domain-containing protein 1 / Myoclonin-1


Mass: 75235.422 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9T8
#39: Protein
Protein SPMIP9 / Sperm microtubule inner protein 9 / Testis-expressed sequence 37 protein / Testis-specific ...Sperm microtubule inner protein 9 / Testis-expressed sequence 37 protein / Testis-specific conserved protein of 21 kDa


Mass: 21069.139 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAG4
#40: Protein
Tektin bundle-interacting protein 1


Mass: 23987.262 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A6H6Q4
#42: Protein ...
Tektin-5


Mass: 62817.535 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: G5E8A8
#44: Protein Stabilizer of axonemal microtubules 4 / Protein phosphatase 1 regulatory subunit 32


Mass: 47873.078 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q148A4
#45: Protein Testis-specific serine/threonine-protein kinase 6 / TSK-6 / TSSK-6 / Testis-specific kinase 6 / Serine/threonine-protein kinase SSTK / Small ...TSK-6 / TSSK-6 / Testis-specific kinase 6 / Serine/threonine-protein kinase SSTK / Small serine/threonine kinase


Mass: 30332.943 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: Q925K9, non-specific serine/threonine protein kinase
#47: Protein
Ciliary microtubule associated protein 1A / Outer dense fiber of sperm tails protein 3 / Outer dense fiber protein 3 / Sperm tail protein SHIPPO 1


Mass: 27690.973 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q920N1
#48: Protein
Parkin coregulated gene protein homolog / Hypertension-related protein 1-like protein / PARK2 coregulated gene protein


Mass: 27763.268 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAK2
#51: Protein
Tektin-like protein 1 / Coiled-coil domain-containing protein 105


Mass: 57406.484 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D4K7
#53: Protein
Enkurin


Mass: 29587.521 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6SP97
#55: Protein
Protein Flattop / Cilia- and flagella-associated protein 126


Mass: 20575.123 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6P8X9
#57: Protein Cilia- and flagella- associated protein 210 / Coiled-coil domain-containing protein 173


Mass: 65266.520 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A0JLY1

+
EF-hand calcium-binding domain-containing protein ... , 2 types, 6 molecules BaBbBci2i3i4

#13: Protein EF-hand calcium-binding domain-containing protein 3


Mass: 49795.141 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q80X60
#56: Protein EF-hand calcium-binding domain-containing protein 6 / DJ-1-binding protein / DJBP


Mass: 176028.312 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6P1E8

+
Ciliary microtubule inner protein ... , 5 types, 26 molecules EaFaFbFcFdGaHIJKLMNO7O8O9P8P9Z2Z3Z4Z5M1M2M3M4

#18: Protein Ciliary microtubule inner protein 1 / Uncharacterized protein C20orf85 homolog


Mass: 15609.958 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DA42
#19: Protein
Ciliary microtubule inner protein 3


Mass: 23717.516 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8K168
#21: Protein Ciliary microtubule inner protein 4 / Testis-expressed protein 33


Mass: 30278.754 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9J2
#22: Protein
Ciliary microtubule inner protein 2A


Mass: 36659.566 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D4K5
#31: Protein
Ciliary microtubule inner protein 2C


Mass: 23097.957 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAS2

+
Piercer of microtubule wall ... , 2 types, 2 molecules Gy

#20: Protein Piercer of microtubule wall 2 protein


Mass: 13728.513 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: V9GXK1
#59: Protein Piercer of microtubule wall 1 protein / Pierce1 / p53-induced expression in RB-null cells protein 1


Mass: 18862.852 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q5BN45

+
Sperm-associated microtubule inner protein ... , 3 types, 10 molecules LaLba1a2a3a4h1h2h3h4

#30: Protein Sperm-associated microtubule inner protein 4 / Protein TISP74


Mass: 67755.727 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D5Y0
#50: Protein
Sperm-associated microtubule inner protein 5


Mass: 25847.469 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CQT6
#54: Protein
Sperm-associated microtubule inner protein 10 / Testis-expressed protein 43


Mass: 16305.608 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9I1

+
Sperm microtubule inner protein ... , 4 types, 16 molecules NaNbNcNdNeOaObOcOdOeOfOgOhP1P2ke

#33: Protein
Sperm microtubule inner protein 8 / Testis / prostate and placenta-expressed protein


Mass: 24985.697 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6IMH0
#37: Protein
Sperm microtubule inner protein 6 / Ciliated bronchial epithelial protein 1 / Spermatid-specific manchette-related protein 1 / Testis ...Ciliated bronchial epithelial protein 1 / Spermatid-specific manchette-related protein 1 / Testis development protein NYD-SP22


Mass: 29977.191 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q2MH31
#38: Protein Sperm microtubule inner protein 11 / Testis-expressed protein 49


Mass: 15528.622 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8CDT5
#60: Protein Sperm microtubule inner protein 2


Mass: 22372.723 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: E9QAV2

+
RIB43A-like with coiled-coils protein ... , 2 types, 5 molecules OPQRS

#34: Protein RIB43A-like with coiled-coils protein 1


Mass: 45032.160 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D0B8
#41: Protein RIB43A-like with coiled-coils protein 2


Mass: 45328.156 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D4Q1

+
Non-polymers , 4 types, 510 molecules

#61: Chemical...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 167 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#62: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 170 / Source method: obtained synthetically / Formula: Mg
#63: Chemical...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 170 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#64: Chemical ChemComp-ATP / ADENOSINE-5'-TRIPHOSPHATE


Mass: 507.181 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C10H16N5O13P3 / Comment: ATP, energy-carrying molecule*YM

+
Details

Has ligand of interestY
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: doublet microtubule of mouse sperm flagella / Type: COMPLEX / Entity ID: #1-#60 / Source: NATURAL
Source (natural)Organism: Mus musculus (house mouse)
Buffer solutionpH: 7.2
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2800 nm / Nominal defocus min: 1300 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

-
Processing

EM softwareName: PHENIX / Version: 1.20.1_4487: / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 83043 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0121638427
ELECTRON MICROSCOPYf_angle_d1.1492221377
ELECTRON MICROSCOPYf_dihedral_angle_d9.282223789
ELECTRON MICROSCOPYf_chiral_restr0.056242709
ELECTRON MICROSCOPYf_plane_restr0.009289519

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more