EMDB-35823: Cryo-EM structure of the 48-nm repeat doublet microtubule from mo...

Basic information

Entry

Database: EMDB / ID: EMD-35823

• Title: Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm

Sample

• Complex: Mouse sperm DMT
  • Protein or peptide: x 49 types
• Ligand: x 3 types

• Keywords: doublet microtubule / STRUCTURAL PROTEIN

Function / homology

Function:

male germ-line stem cell population maintenance / protein localization to motile cilium / left/right pattern formation / axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / outer dense fiber / Carboxyterminal post-translational modifications of tubulin / Intraflagellar transport / protein polyglutamylation / inner dynein arm assembly / regulation of calcineurin-NFAT signaling cascade / sperm axoneme assembly / regulation of microtubule nucleation / positive regulation of feeding behavior / COPI-independent Golgi-to-ER retrograde traffic / Transferases; Transferring phosphorus-containing groups / sperm principal piece / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / regulation of cilium beat frequency involved in ciliary motility / MAP kinase tyrosine/serine/threonine phosphatase activity / cerebrospinal fluid circulation / epithelial cilium movement involved in extracellular fluid movement / cilium movement involved in cell motility / COPI-mediated anterograde transport / Aggrephagy / 9+2 motile cilium / Kinesins / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / protein localization to organelle / regulation of store-operated calcium entry / intraciliary transport / Resolution of Sister Chromatid Cohesion / PKR-mediated signaling / acrosomal membrane / The role of GTSE1 in G2/M progression after G2 checkpoint / axoneme assembly / RHO GTPases activate IQGAPs / microtubule sliding / Recycling pathway of L1 / cilium movement / ciliary transition zone / left/right axis specification / calcium ion sensor activity / axonemal microtubule / COPI-dependent Golgi-to-ER retrograde traffic / sperm DNA condensation / RHO GTPases Activate Formins / Separation of Sister Chromatids / cilium organization / Hedgehog 'off' state / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / gamma-tubulin ring complex / Regulation of PLK1 Activity at G2/M Transition / manchette / positive regulation of cilium assembly / flagellated sperm motility / MHC class II antigen presentation / cell projection organization / UTP biosynthetic process / CTP biosynthetic process / determination of left/right symmetry / intermediate filament / protein targeting to membrane / extrinsic component of membrane / positive regulation of cell motility / nucleoside diphosphate kinase activity / protein targeting to mitochondrion / GTP biosynthetic process / tubulin complex / AMP binding / protein-serine/threonine phosphatase / ciliary base / receptor clustering / protein serine/threonine phosphatase activity / phosphatase activity / regulation of cell division / microtubule organizing center / phosphoprotein phosphatase activity / mitotic cytokinesis / cellular response to UV-C / axoneme / cilium assembly / glial cell projection

Domain/homology:

Spermatid-specific manchette-related protein 1 / Testis-expressed sequence 37 protein / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / : / Spermatid-specific manchette-related protein 1 / TSC21 family / Sperm-associated microtubule inner protein 5 / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Sperm microtubule inner protein 8 / Sperm microtubule inner protein 11 / : / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / : / Cilia- and flagella-associated protein 68 / Sperm-associated microtubule inner protein 10 / Protein of unknown function DUF4541 / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Tektin bundle interacting protein 1 / Cilia and flagella associated protein 90 / : / Sperm-tail PG-rich repeat / DJBP, EF-hand domain / FAM183 family / Sperm-tail PG-rich repeat / EF-hand domain / FAM183A and FAM183B related / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Protein CFAP95 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / : / Tektin family / Protein of unknown function (DUF3695) / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 161-like domain / : / EFHB C-terminal EF-hand domain / : / Nucleoside diphosphate kinase 7 N-terminal PH domain / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dual specificity phosphatase, catalytic domain / EF hand / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity protein phosphatase domain profile. / Dual specificity protein phosphatase domain / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase (NDPK)-like domain profile. / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / EF-hand domain pair / Mir domain superfamily

Component:

Cilia- and flagella-associated protein 77 / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Cilia- and flagella-associated protein 144 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Stabilizer of axonemal microtubules 4 / Tektin-4 / Sperm microtubule inner protein 6 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 107 / Piercer of microtubule wall 1 protein / Cilia- and flagella-associated protein 52 / Meiosis-specific nuclear structural protein 1 / Sperm microtubule inner protein 8 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Cilia- and flagella-associated protein 161 / Enkurin / Tektin-3 / Cilia- and flagella-associated protein 20 / Sperm microtubule inner protein 11 / EF-hand domain-containing family member B / Ciliary microtubule associated protein 1A / Tektin-2 / Testis-specific serine/threonine-protein kinase 6 / Cilia- and flagella-associated protein 95 / Sperm-associated microtubule inner protein 5 / RIB43A-like with coiled-coils protein 1 / Cilia- and flagella-associated protein 68 / Cilia- and flagella-associated protein 53 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 2A / Tektin-like protein 1 / RIB43A-like with coiled-coils protein 2 / Dual specificity phosphatase 21 / Cilia- and flagella-associated protein 141 / Sperm-associated microtubule inner protein 10 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 45 / Cilia- and flagella-associated protein 276 / Protein SPMIP9 / Tektin-1 / Parkin coregulated gene protein homolog / Cilia- and flagella-associated protein 90 / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase homolog 7 / Piercer of microtubule wall 2 protein

• Biological species: Mus musculus (house mouse)
• Method: single particle reconstruction / cryo EM / Resolution: 3.5 Å
• Authors: Zhou LN / Gui M / Wu JP

Funding support

China, 1 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)		China

• Citation: Journal: Cell / Year: 2023; Title: Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility.; Authors: Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu / Miao Gui / Jianping Wu / ; Abstract: Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility.

History

Deposition	Apr 5, 2023	-
Header (metadata) release	Jul 5, 2023	-
Map release	Jul 5, 2023	-
Update	Jul 3, 2024	-
Current status	Jul 3, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_35823.map.gz / Format: CCP4 / Size: 689 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.087 Å

Density

Contour Level	By AUTHOR: 0.3
Minimum - Maximum	0.0 - 2.8214865
Average (Standard dev.)	0.0727588 (±0.17619546)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 204 133 0 Dimensions 426 530 800 Spacing 530 426 800
Cell	A: 576.11 Å / B: 463.062 Å / C: 869.60004 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Mouse sperm DMT

• Entire: Name: Mouse sperm DMT

Components

• Complex: Mouse sperm DMT
  • Protein or peptide: Cilia- and flagella-associated protein 95
  • Protein or peptide: EF-hand domain-containing family member B
  • Protein or peptide: Cilia- and flagella-associated protein 53
  • Protein or peptide: Nucleoside diphosphate kinase 7
  • Protein or peptide: Cilia- and flagella-associated protein 107
  • Protein or peptide: Cilia- and flagella-associated protein 141
  • Protein or peptide: Tektin-1
  • Protein or peptide: Tubulin alpha-3 chain
  • Protein or peptide: Tubulin beta-4B chain
  • Protein or peptide: Meiosis-specific nuclear structural protein 1
  • Protein or peptide: Tektin-2
  • Protein or peptide: Tektin-3
  • Protein or peptide: Sperm-associated antigen 8
  • Protein or peptide: Tektin-4
  • Protein or peptide: Cilia- and flagella-associated protein 161
  • Protein or peptide: Piercer of microtubule wall 2 protein
  • Protein or peptide: Protein FAM166A
  • Protein or peptide: Cilia- and flagella-associated protein 68
  • Protein or peptide: Cilia and flagella-associated protein 77
  • Protein or peptide: Family with sequence similarity 183, member B
  • Protein or peptide: Cilia- and flagella-associated protein 90
  • Protein or peptide: Protein FAM166C
  • Protein or peptide: RIB43A-like with coiled-coils protein 1
  • Protein or peptide: Dual specificity phosphatase 21
  • Protein or peptide: EF-hand domain-containing protein 1
  • Protein or peptide: Testis expressed 49
  • Protein or peptide: Testis-expressed sequence 37 protein
  • Protein or peptide: Tektin bundle-interacting protein 1
  • Protein or peptide: RIB43A-like with coiled-coils protein 2
  • Protein or peptide: Tektin-5
  • Protein or peptide: EF-hand domain-containing family member C2
  • Protein or peptide: Spermatid-specific manchette-related protein 1
  • Protein or peptide: Protein phosphatase 1 regulatory subunit 32
  • Protein or peptide: Testis-specific serine/threonine-protein kinase 6
  • Protein or peptide: Cilia- and flagella-associated protein 20
  • Protein or peptide: Outer dense fiber protein 3
  • Protein or peptide: Parkin coregulated gene protein homolog
  • Protein or peptide: Testis, prostate and placenta-expressed protein
  • Protein or peptide: Cilia- and flagella-associated protein 45
  • Protein or peptide: Uncharacterized protein C10orf82 homolog
  • Protein or peptide: Coiled-coil domain-containing protein 105
  • Protein or peptide: Cilia- and flagella-associated protein 52
  • Protein or peptide: Enkurin
  • Protein or peptide: Testis-expressed protein 43
  • Protein or peptide: Protein Flattop
  • Protein or peptide: EF-hand calcium-binding domain-containing protein 6
  • Protein or peptide: Cilia- and flagella-associated protein 210
  • Protein or peptide: Cilia- and flagella-associated protein 276
  • Protein or peptide: Piercer of microtubule wall 1 protein
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: Mouse sperm DMT

• Supramolecule: Name: Mouse sperm DMT / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#49
• Source (natural): Organism: Mus musculus (house mouse)

Macromolecule #1: Cilia- and flagella-associated protein 95

• Macromolecule: Name: Cilia- and flagella-associated protein 95 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 26.633035 KDa

Sequence

String:

MGTFDSQCHE CFESKERWYE IGPTDLLERK GSLTLRSHHK KYSEPVLVYS WHRNREAFPK DYDIESLETV KNLCMSTYKR FGSDSPRWM SEAREQMAQV LVNKDLVEKK KTGLLDEETL CPGILERDPE LPPTGFRDVP MESPPDPRKP CFSTTYSEDF T PQYEYPPP ACPRREEYSI VHRKCRSQFT DLNGSKRLGI NTWHDESGIY ANSEAKQKLY ALARNPIVPF

UniProtKB: Cilia- and flagella-associated protein 95

Macromolecule #2: EF-hand domain-containing family member B

• Macromolecule: Name: EF-hand domain-containing family member B / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 95.891961 KDa

Sequence

String:

MCSFVRVGSP KPLQTSASPL EMSSLRRTRA PEISELGLTP EQKDDIRDRV LRGSKSPTEL GFDLRLEQDR KWRERMGSSE AKSPPCHAL GVGLERHTIS GTPTEMGNLG LHKGSAFQGS KPLGVLPGRV GPENKGLPPR LRYGGTLHPP FSTVHASPLA A ESRRRPLA WGSAWTDAVV EKQPVVGLEL RKEPEKEPTC VVMNPYPEMP PKEVDIGLPQ TQESDEAKNT EPLIGLVREP SE CPFAQQP EEKKEPGSTE PGVEPPGNIR PIYSGKFFDR VPCWPSAGKV KPVGYRVATC LTEKLPRLMT PPEAKKYFNF RYP PAGAER VFYGRANDPQ IAPYLTHGLR SKISIPMGSL INPQPITTFQ QKIKDKKESI YFSHQRAPLG KSHDQTPGLP KGMD VINTT LGTPTIRELS VRDTVNPSKS FEDVLKEGQE GHDLYTVSHN DYFAGEAKNR KYNPASFHRF NLYGIPTPHF NDGRT MAKA LHWLHELQME RGAKIVSKRV DDFKEKFQHK LGKVLDPIAE TMNVPPGHTF GSCLHPEEYG AGDLIHYRSP DEYLRG KDH QRAVVAAARH HLKKFNHQNF DTLQVAFRHY DKKGDGVIDR AELHEACVQA NLHLDKMLLD HLFDYCDVDQ DGLINYL EF ANFLNWKDRI PLKEHEKRVV VKGKKPDCEN VTDTSMGEAE PSLLINPEDI VPKEPGSSEE TLRTIQRPGD KVSHQYKT T SSEINAVVGA VPSMCHPIFG VPTIRSDISA PRIRRVSDMN NYGDEGNAYS LLHPSIFSQK GVFERDFFKT RSKEEISDI LTNIGVKLSK EEFENVWNLA SKKHQRGEVC VETIRNVLDE LLHADLVKCK TAM

UniProtKB: EF-hand domain-containing family member B

Macromolecule #3: Cilia- and flagella-associated protein 53

• Macromolecule: Name: Cilia- and flagella-associated protein 53 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 62.036609 KDa

Sequence

String:

MYSQQFGTVP REFKGPTPKA VIIRAKPPKA QRAEQHLKRI QRSYHKYHTT LASIKSNEEN RLKCDWIQRN NHKTFDSLVQ ARVQDAMQG FVINTEERRN KLRELLASEE NEYFSEMQLK GETIEEKKDK MRERTKLLRE KKEKERQEFV AEKLDQQFRE R CEELRTKL ASIHEKKVVE ERNAQIEFNK ELKRQKLVEE HLFARLWEED RLAKERREAQ EEKRQRELVQ NTRLGLDAQV TS IQAQRQG ARRMKEEEAR ILEQNKAQIK REDEQEKLQK QKRRQETRSS LKKAVQDKIE SMQREYREDL DLNMKLVGRA LQD LQDEAD KKKQKREEMG REQKIYNDYL MQRREEEKAQ EKELNRLLED IKAKKLAEKD RELALQRAAR KQLMNEVMNT RKLQ VQERL QRKLREQEEL ALHEQRISES LKVLHQEDME DFARRCALAE EYRNQLQMQI AHQQQAREAE KEEERQEFEA GLAAN KACL DKIQRILSEN QALSQNVHPM RRGYPDKPPL

UniProtKB: Cilia- and flagella-associated protein 53

Macromolecule #4: Nucleoside diphosphate kinase 7

• Macromolecule: Name: Nucleoside diphosphate kinase 7 / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 44.489828 KDa

Sequence

String:

MRACQQGRSS SLVSPYMAPK NQSERFAFIA EWYDPNASLL RRYELLFYPV DGSVEMHDVK NRRTFLKRTK YEDLRLEDLF IGNKVNVFS RQLVLIDYGD QYTARQLGSR KEKTLALIKP DAVSKAGEII EMINKSGFTI TKLRMMTLTR KEAADFHVDH H SRPFYNEL IQFITSGPVI AMEILRDDAI CEWKRLLGPA NSGLSRTDAP GSIRALFGTD GVRNAAHGPD TFASAAREME LF FPSSGGC GPANTAKFTN CTCCIIKPHA ISEGMLGKNL IAIRDACFGM SAIQMFNLDR ANVEEFYEVY KGVVSEYNDM VTE LCSGPC VAIEIQQSNP TKTFREFCGP ADPEIARHLR PETLRAIFGK TKVQNAVHCT DLPEDGLLEV QYFFKILDN

UniProtKB: Nucleoside diphosphate kinase homolog 7

Macromolecule #5: Cilia- and flagella-associated protein 107

• Macromolecule: Name: Cilia- and flagella-associated protein 107 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.06251 KDa

Sequence

String:

MAMLSTSVVP EAFSTPGWQI EKKYSTKVLL GNWVEERGKF TKAIDHTPQC IYRKEYVPMP DHRPDFVSRW YSKSKMEGLP YKHLITHHQ EPSHRYLIST YDDHYNRHNY NPGLPALRTW NGQKLLWLPE KSDFPLVAPP TNYGLLEQLQ QKWLASKTSL K ESIYTTSY PRLPVCAMSR REHAIPVPHP RLQPIPRF

UniProtKB: Cilia- and flagella-associated protein 107

Macromolecule #6: Cilia- and flagella-associated protein 141

• Macromolecule: Name: Cilia- and flagella-associated protein 141 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.278145 KDa

Sequence

String:

MSAEKMTKLE ENLQRAVALK KTVDRWRNFH IHCMWQTTLD QRRNLFAALR MKDTKEQELA LSNKQLLVVR QAALHELFEK EYQQYQQEL NQMGKAFYEE RL

UniProtKB: Cilia- and flagella-associated protein 141

Macromolecule #7: Tektin-1

• Macromolecule: Name: Tektin-1 / type: protein_or_peptide / ID: 7 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 48.713035 KDa

Sequence

String:

MAKLLQSPPR FLPEEWYIAN KSQYHRAEAQ RSQSERLVAE SQRLVEEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVNQTD DLLTYKTRLE RSLESYKEPL HITEKCLEYR EKRVGIDLVH DVVEQELQKE ADIIHGVMNL LIRTLEESTE Q IRLNRSAK YNLEKDLRDK FTAITIDDVC FSLNNNSPNI NFSEKVVRIE PNSVSLEDWL DFSNANVEKA DKQLNNSTAL KT LVDQILS QTANDLRRQC EVVDEAFING LKETKDARNK LADHLAKVME EIASQEKNIM ALENAITQQE GPAKVAHTRL ETR THRPNV ELCRDIAQYR LIKEIQEINH NVARLKETLA QAQTQLKALY RRQLALQEEI QVKENTIYID QVLCMEMRKS IPPR DGDDH GAWEGGIRAE AIC

UniProtKB: Tektin-1

Macromolecule #8: Tubulin alpha-3 chain

• Macromolecule: Name: Tubulin alpha-3 chain / type: protein_or_peptide / ID: 8 / Number of copies: 178 / Enantiomer: LEVO; EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.012359 KDa

Sequence

String:

MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVVDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIVDLVL DRIRKLADLC TGLQGFLIFH SFGGGTGSGF ASLLMERLSV D YGKKSKLE FAIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCM LYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GVDSVEAEAE EGEEY

UniProtKB: Tubulin alpha-3 chain

Macromolecule #9: Tubulin beta-4B chain

• Macromolecule: Name: Tubulin beta-4B chain / type: protein_or_peptide / ID: 9 / Number of copies: 176 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 49.877824 KDa

Sequence

String:

MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEAEEEVA

UniProtKB: Tubulin beta-4B chain

Macromolecule #10: Meiosis-specific nuclear structural protein 1

• Macromolecule: Name: Meiosis-specific nuclear structural protein 1 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 60.353012 KDa

Sequence

String:

MATKKRALSF SEKHQKLVDE KFRKSLNIQV MNKLERQAKN QVVQNENDEK VERQRFLRVL QNEQFELDME EAIQKAEANK MLRDRQLEQ EERLANELAR LKHESLKDKK MRQQVRENSI ELRELEQKLK AAYMNKERAA QIVEKDAMKY EQMKRDAEIE R IMMEEHDR LLKEESAKQE RRNKERAQYY LDLEKQLEDQ ERRKQEAYEQ LLKEKLMIDE IVRKIYEEDQ VERQQKLEKK NA IQKYIEE FQRAQDFWRQ KKREEMEEEN RKIIEFANIQ EQREGERMAR VHEIEEKRVQ RQNLLMKQLE ETLRQRDDLE QVR QELYQE EQAEIIKLKV KEEAELRLRR QREMKQDFED QMALKELILQ AAKEEEETFK KAMLAKFAED DRIELMNAQK QRMK QLEHK RAVEKLIEER RSQFLADKQR ELEELQLQQR RQGCINEIIE EERLRLLKEH AAKLLGYLPK GVFKREDDVD MLGEE FRKA YQKRDGV

UniProtKB: Meiosis-specific nuclear structural protein 1

Macromolecule #11: Tektin-2

• Macromolecule: Name: Tektin-2 / type: protein_or_peptide / ID: 11 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.385066 KDa

Sequence

String:

MATLSFKPSE RYRLSDWRTN SYLLSTNAER QRDASHQIRQ EARILRNETN NQIVWDEHDN RTRLAERIDT VNRWKETLDK CLTDLDAEI DSLAQAKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV RDPVEEELLK EVEVIEATKK VLQEKISQAF Q HLCLLQEI RQQLNSDHRD KMETLEIDRG CLSLNLTSPN ISLKVNPTRI PKDSTTLQQW DEFTRFNKNR AEAEMKASIE LR EAIALAI AQTNNELDAQ RVATEFTFRK RLREMESFYS ELKWQEKNTL EEIAELQGDI RRLEEDLRRK MMNLKLAHTR LES RTYRSN VELCRDQTQY GLIDEVHQLE ATINTMKQKL AQTQNALDAL FKHLARIQAD IACKTNTLLL DTKCMDTRRK LTVP AEKFV PQVDTFTRTT NRTLSPLKIC QLELT

UniProtKB: Tektin-2

Macromolecule #12: Tektin-3

• Macromolecule: Name: Tektin-3 / type: protein_or_peptide / ID: 12 / Number of copies: 17 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 56.754777 KDa

Sequence

String:

MELLGSTLTA TYAHPPPASA SFLPAIGTIT SSYKDRFPHR NLTHSLSLPW RPNTYYKTAY NYPTLAPYSS RSQRVCESTM LPFVSNRTT FFTRYTPDDW YRSNLVSFQE SNSSRHNSER LRVDTSRLIQ DKYQQIRKTQ AHSTQNLGER VNDLAFWKSE I THELDEMI GETNALTDIK RRLERGLIET EGPLQVSREC LFHREKRMGI DLVHDEAEKE LLAEVDTILC CQERMRQHLD KA NAQLASD RSAQHELEKD LSDKQAALRI DDKCQHLRNT SEGVSYFRGV ERVDATVSVP ETWAKFTDDN VLRSQSERAA SAK LREETE NLLIVTANEM WNQFNKVNLA FTNRIAETVD AKNKIHTHLT KTLQEIFQIE MTIESIKKAI KEKSAFLKVA QTRL DERTR RPNVELCRDM AQLRLVNEVY EVDETIQTLQ QRLRDSEDTL QSLAHTKATL EHDLAVKANT LYIDQEKCMS MRNSY PSTL RLVGYC

UniProtKB: Tektin-3

Macromolecule #13: Sperm-associated antigen 8

• Macromolecule: Name: Sperm-associated antigen 8 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.591805 KDa

Sequence

String:

METTESTEGS LSRSCDVQPS SERLDTPSEP VPSSSSSPRS TAPAEAPAQY SVLTEPSSDS LYGAPCPPAH HRGHGFGFQP FYVSCIPQD PCNMADLSSR ADPTSSYPCH SSVHGSGSGT CGLGQSSEPS QGSGPTSGPA PASVPSLVSG PDSASGPDSS A SGPALASG PGPADPGQGP KFSTCIPQGY RCIPVDLAPD YNAWCQHLHW KPQRSWEPLQ VSEPGVRGPY KPPEPGALGP CE PCEPCEP PEAESEETLC KARPRGQCLL YNWEEERATN QLDQIPPLQD GSESYFFRHG HQGLLTTQPQ SPMSSSTTQR DSY QLPRHI CQPLRGKREA MLEMLLRHQI CKEVQAEQEP ARKLFETESV THHDYRVELV RAAPPASTKP HDYRQEQPET FWIQ RAARL PGVSDIRTLD TPFRKNCSFS TPVPLSLGQP LPYELESGPH QVGVISSLAC QGGGQGCGRT KTTPI

UniProtKB: Sperm-associated antigen 8

Macromolecule #14: Tektin-4

• Macromolecule: Name: Tektin-4 / type: protein_or_peptide / ID: 14 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 52.121449 KDa

Sequence

String:

MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK YLRDEWFQNS YARYHQAFAD RDYSERQRHE SGQLVAETG ALAQRTQLDS TRKVGERLED MHCWKSELQR EIDELSSETD LMMAQKLRLQ RALDATSVPY SIATDNLQCR E RRQHPDLV RDYVEVELLK ETELIRNIQE LLKRTIGQAV DQIRLNREHK ESCEMNWSDK VEVYNIDDTC SRYTNESTQV QF YPHSSKF EESASTPETW AKFNHDNLLR AERERLASVN LRKLIDCILR DTAEDLRLQC DAVNSAFSSR CQELDDSLQK LQY HLRKTL TEITDQEHQI AALKQAIKDK EAPLRVAQTR LYQRSHRPNV ELCRDNAQFR LLSEVEELNM SLRALKEKLQ DAEQ ALRNL EDSRMSLEKD IAVKTNSLFI DRQKCMTHRN RYPSVLQLAG YQ

UniProtKB: Tektin-4

Macromolecule #15: Cilia- and flagella-associated protein 161

• Macromolecule: Name: Cilia- and flagella-associated protein 161 / type: protein_or_peptide / ID: 15 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 34.433383 KDa

Sequence

String:

MAQNVYGPGV RMGNWNEDVY LEEERMRHFL EKREKGELLI QRNRRVKKNI LRPMQLSVSE DGYVHYGDKV IIVNPDQVLG EEAGKFMRG DLSLCMSPDE VKAQLSDDLE IPCGVSAVQT IAPMGRNTFT ILSDGANSCE MGQVVVYGQN FCLGIAAGLE G KMLYLTSD HRTLLKSSLK SGLQEVTLTD EVTHLNCWQA AFLDPQLRLE YEGFPVRANE KIVIYHRHTN RALAVHRNLF LR TYFGKEM EVVAHTYLDS HKVEKPKNQW MLVTGNPRNK SNTMLDISKP ITEDTRALEQ AMGINT

UniProtKB: Cilia- and flagella-associated protein 161

Macromolecule #16: Piercer of microtubule wall 2 protein

• Macromolecule: Name: Piercer of microtubule wall 2 protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.728513 KDa

Sequence

String:

MARETDCDLD KKTSLTSDAE MRPEPPALCV NPGNPVFSCM LDPKTLHTAT SLSKAQMIMY KTSASQYGAF SPRPFFFPCK FLPQEQAFT EHLKTTGFYQ NNSLNVGPDR TRTIDSPNYQ HTL

UniProtKB: Piercer of microtubule wall 2 protein

Macromolecule #17: Protein FAM166A

• Macromolecule: Name: Protein FAM166A / type: protein_or_peptide / ID: 17 / Number of copies: 16 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 36.659566 KDa

Sequence

String:

MTATQKHNLF TPEPHYIPGY AGFYPQLRYQ VGNTYGRTTA QLLTDPSVQK SPCSVLSPMT KPKFIEDFSK SKPPWIPCRD LREPYIPHY TSLKPYKNFE ILGQLPRQDV DTQGPPQVEN RQGPLTAGFM PYPPYPACPP GRKGEARDLG HPGLLLAYGE E AWKDAAPL QDTPGKNNQL YHCRRDEYLP PHPPQETLDV GRFQRLPQLD HPNLIQRKAI SGYAGFVPRF AWVMGMNYRD GV TQAMDDF DKNQFVFRHP VCALGERLPR THWPNTTIYR SQGLIPFYMG FIPSMQDNYA LTFGNSTRRA YQKELDRRSH TL

UniProtKB: Ciliary microtubule inner protein 2A

Macromolecule #18: Cilia- and flagella-associated protein 68

• Macromolecule: Name: Cilia- and flagella-associated protein 68 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 19.552668 KDa

Sequence

String:

MAVSCSLNHS TYLQRQNLVC YLRNPHYGSL IYADGHGEVW TDWNDMSKFL QYGWRCTTNE NSYSNRTLVG NWNQERYDLK NIVKPKPLP SQFGHAFETT YDANYSRKKP QSTHRFKREP HWFPGHQPEL DPPHYKCTAK STYMTNYSEP QPTHYSCCVY D PSVSQS

UniProtKB: Cilia- and flagella-associated protein 68

Macromolecule #19: Cilia and flagella-associated protein 77

• Macromolecule: Name: Cilia and flagella-associated protein 77 / type: protein_or_peptide / ID: 19 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 32.78602 KDa

Sequence

String:

MPDPAKPGKD LTAWKKKKQP VHRTVSQICP PPRRPLTVVD IRTGMENERL GVVRDSMFQN PLIVKAELGK PRERSCSLPG INFNYGLYI RGLDGGVPEA IGHWNVFKQQ PTCPHELTRN YIAMNRGAVK AGLVTARENM LYRELNDIRI NDQEDRRQKE P PPIPPNMT FGIRSRPSTP FFDLLQHRYQ QLWVQEQKAT QQAIKMEKKQ KVILGKLYET RSSQLRKYKP PVKLDALWHM PH FKKVASH LATFPTEADR QRALKAHKEE YAVRQGTLRM GNYTHP

UniProtKB: Cilia- and flagella-associated protein 77

Macromolecule #20: Family with sequence similarity 183, member B

• Macromolecule: Name: Family with sequence similarity 183, member B / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.357517 KDa

Sequence

String:

MAMAGRVGQM KNQDEVHQNQ ILRELFLKEL RAQKLYTQYH VNPLRKVHTI TRKPMSWHDN LEEPEDAKFL NLIHHAAQGP KKKYSETQT EAQEIGWDPN PLINPDRQDH RLNHFRVYHD ITLYKAKLWS LGEDDHQK

UniProtKB: Cilia- and flagella-associated protein 144

Macromolecule #21: Cilia- and flagella-associated protein 90

• Macromolecule: Name: Cilia- and flagella-associated protein 90 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.886428 KDa

Sequence

String:

MEELAKKERR AMDPGGLKKE GKVEEEAGKE EGREEEGGEE EEVTSETLRG KPRPLPISAL PAFSYIPPRH QGPKERSYFS REGQTGIVS LYDCVFKRRL DYNQKLHRDD REHAKNLGLH INEEEQERTV PVLMSSVYGK RINQPIEPLN RDYGHVSHVK T DFYRKNEI PSIKGPGFGH INPA

UniProtKB: Cilia- and flagella-associated protein 90

Macromolecule #22: Protein FAM166C

• Macromolecule: Name: Protein FAM166C / type: protein_or_peptide / ID: 22 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.097957 KDa

Sequence

String:

MAFRSAGTLM TEFNAAFVPP ALMPGYKGHV PGVASSFGSS YGNTTFKYFQ DLRNTGLEKS YALLSGGCFP TIFSPNSPLV LTDNSQNWD RWLHQPTYSR SNQDNGRTTE LTNFYKTVQQ QRKYYQDKTG ILSRVPYFVL PVKEPDRYPL PTDLPPLSEK S KWNLLRLS PDYKRTYQTF PSGKRVSSQE RQRRDLYFEF RA

UniProtKB: Ciliary microtubule inner protein 2C

Macromolecule #23: RIB43A-like with coiled-coils protein 1

• Macromolecule: Name: RIB43A-like with coiled-coils protein 1 / type: protein_or_peptide / ID: 23 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 45.03216 KDa

Sequence

String:

MYKLDLVSDP RELAAIEARR NREKERQSRF FNVRNRVMGV DVEALNYQVE ERKFREAIER NKDIAYGTKH AHYDLVAQML EKEEAERAY RLSKRVQDFR EQRQQQYKNG HEFDFWDPDQ FQEFRVPYYE KDAYFGPASM QYFFGEDLER ASHVRMQQEQ M RYNLEKQL QEQQAAREEE ARAALLSDQL RLAADTRAAE LARLEESCRA AMRTAMANAN KAQAAKQALQ QRREQQRQQE AN LTEVKKQ VTSDLLTENP QVAQRANAPH RVLPYCWKGM SAEQRAAIRK TQETQRQEKK EQRQAEKLVE AEWGRQNKRL AEA ALELEE QERELCAEFR RGLGSFNREL AKEQQAQQNY LNSVIYTNQP TAHYYLQFNT SSR

UniProtKB: RIB43A-like with coiled-coils protein 1

Macromolecule #24: Dual specificity phosphatase 21

• Macromolecule: Name: Dual specificity phosphatase 21 / type: protein_or_peptide / ID: 24 / Number of copies: 4 / Enantiomer: LEVO / EC number: protein-serine/threonine phosphatase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.527574 KDa

Sequence

String:

MTTASCIFPS QATQQDNIYG LSQITASLFI SNSAVANDKL TLSNNHITTI INVSAEVVNT FFEDIQYVQV PVSDAPNSYL YDFFDPIAD HIHGVEMRNG RTLLHCAAGV SRSATLCLAY LMKYHNMTLL DAHTWTKTCR PIIRPNNGFW EQLIHYEFKL F SRNTVRMI YSPIGLIPNI YEKEAYLMEL M

UniProtKB: Dual specificity phosphatase 21

Macromolecule #25: EF-hand domain-containing protein 1

• Macromolecule: Name: EF-hand domain-containing protein 1 / type: protein_or_peptide / ID: 25 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 75.235422 KDa

Sequence

String:

MGTNPVHGLP FLPGSSFTDS TKTAFHRSQT LNYRNGYAVV RRPTMGIGGD RLHYNQLSQA ELDELANKAP ILTYGPLKQA PLAEFVPAH VAFDKKVLKF SAYFQEDVPI SMEEHYRIRH VNIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRFTKNDM G DHYHWKDL NRGINLTVYG KTFRIVDCDR FTQDFLESQG IELNPSEKIP LDPYTQLRKE PVRKYVTPSD FDQLKQFLTF DK QVLRFYA IWDDTDSLFG ECRHYIIHYY LMDDTVEIRE VHERNNGRDP FPLLMNRQRM PKVLVENAKN FPKCVLEISD QEV LEWYTA KDFIVGKPLT ILGRTFFIYD CDPFTRQFYK DKFGMPDLPP VDVTKKEPPP VKQELPPYNG YGLIEDSAQN CFAL IPKAP RKDVVKMLMN DNKVLRYLAA LESPIPEDKD RRFVFSYFLA TDMISIFEPP VRNSGIIGGK FLGRTKVVKS FSPVD NPIY YSPSDFFIGA VIEVFGHRFV ILDTDEYVLK YMESNASQYS PEALASIQNR IQKPELPAPE LESKQATGEP MVQGTE ESK VQDLDALIDQ IHMHLKYNSY KENLRETFQM YDKDESGYVD RETFFKICET LNVPVDDSLI KELIRLCTHG EGRINYY NF VRAFSN

UniProtKB: EF-hand domain-containing protein 1

Macromolecule #26: Testis expressed 49

• Macromolecule: Name: Testis expressed 49 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.528622 KDa

Sequence

String:

MAFFNLYLLG YQHYFQSKKR NTAQETNQKD EVPTRLPPII SEDGNYSVHQ NSHERYQEAV RKVLLKTFPN QVFRVPVTDA QNFSFWRSN IPGVRPEKAI PWIKTPHHCL IKSPMTRFVD HSHLNDKTFS LY

UniProtKB: Sperm microtubule inner protein 11

Macromolecule #27: Testis-expressed sequence 37 protein

• Macromolecule: Name: Testis-expressed sequence 37 protein / type: protein_or_peptide / ID: 27 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.069139 KDa

Sequence

String:

MARVVRPQKN HVDLDIYQSS YMVDYKPFGK YKYSRVTPQE QAKLDAQLQS KEFYQPKPNP NPKLEEGYPA FRRPYMTALD LGVPGFFPP QERVTTRKDD GRFTTTCHYA YPASLALYLA QQDPYWLHQR ADFPCLMEPE RQPAPEVGKG YLLLPGCLCD H HQRVKVPI LNRWGPLMPF YQ

UniProtKB: Protein SPMIP9

Macromolecule #28: Tektin bundle-interacting protein 1

• Macromolecule: Name: Tektin bundle-interacting protein 1 / type: protein_or_peptide / ID: 28 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.987262 KDa

Sequence

String:

MENVRREATR PSVPSGTLEL YFPDHLYRND YVSLEGPRWA PAIKQAVRWK FTPMGRDAAG QVWFTGLTNS EPGDAWYKLP RALDTPYRE AHTRWHGCFQ SRQRGLPPAY TQHLREMAFW DPAITAQYLN SGPRWGCMQW RDRQIRGKEF VVTRNQFGAK L PWRSDYVP LLSLPQRPRF TAQDFRQRGL QRPCPAIGQP PPAFTPAL

UniProtKB: Tektin bundle-interacting protein 1

Macromolecule #29: RIB43A-like with coiled-coils protein 2

• Macromolecule: Name: RIB43A-like with coiled-coils protein 2 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 45.328156 KDa

Sequence

String:

MEVAMSKDLQ QEANLAKKRY IDLCRQGRIF DARNRIIGGD TQAWDFQVRD QKIKEITDKA RHEAFAAEMK HNDKVMCMAH DREQRHRKQ LCRAINDFQQ NFQKPETRRE FDLSDPLALQ KELPARISDN DMRNTISGMQ KFMGEDLNFQ ERRRFQKEQS R EWFLQQHG EREKARADHL LAEHLHTQTR LKFDETAREL MKLEGSTRKE VCAAVKAFNK NQVVELTERK RQEKQQEQED NM TEITNLL HGDLLSENPR PVASSFGSHR VVLDRWKGMN REQLEEIWFT QKRQIQEKLR LQEEERQHSM DWDLRRIRKA HAS LLHERQ QQRLLREQRR ALDCSNLNLA RQQYLQKKQM NTASSSQPTE DYFSQFNTRS R

UniProtKB: RIB43A-like with coiled-coils protein 2

Macromolecule #30: Tektin-5

• Macromolecule: Name: Tektin-5 / type: protein_or_peptide / ID: 30 / Number of copies: 26 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 62.817535 KDa

Sequence

String:

MEFLGTTQTA SFCGPKKGCG LQALPPAGQE PVVQECYQPF HLPGYRYLNA WRPSVFHKIA TSQTIPEECS GIRRPPTILP SLRSALFCR YTPRDWDRSN DLQIRNAEAS RLWASRLTGD SLRIMQDKDQ LIHQMQEGTS RNLGQRLSDL GFWKSELCYE L DRLLTENS SMDTLKRRLE CAAEEVNCPL QVALECLYNR EKRIGIDLVH DNVEKNLIRE VDLLKCCQDQ MRKLAKRIDF QI RDNRDAQ HSLERDIEDK SSAQYIDENC FNLRSTSDSI SFFHGVEKFD GTVSIPETWA KFSNDNIRHA QNMRANSIRL REE AEHLFE TLSDQMWKQF TNTNLAFNAR ISEETDVKNK LQTQLAKILQ EIFQAENTIM LLERAIVAKE YPLKMAQTML ACRT RRPNV ELCRDVPQFR LVNEVFTIDD TLQTLKLRLR ETQDTLQLLV MTKSRLEHEL AIKANTLCID KDKCMSMRKS FPSTP RLTG YTCSAIGSGP YANHAPRISS GPCSGSALCK GPASCGGGAS CGGGASCGGH APCGSALCSH SVSRSGPGFA PVC

UniProtKB: Tektin-5

Macromolecule #31: EF-hand domain-containing family member C2

• Macromolecule: Name: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 31 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 87.758023 KDa

Sequence

String:

MALPFLPGNS FNRNIGKERF HKSQHWGFCN NVRMLVSENK PGVGGDLLYG QKIKPKHSVF PKGDGTDAPS WVAFDKQVLS FDAYLEDEI SDKRQEIFRI RYYKIYFYLE DDTIQVNEPE VINSGLPQGT SIRRQRIPYP PPNDDQFYTV YDFNINISVV F YGRTFKIY DCDPFTKNFL KKIGIKLNPP GQCPLDPYMK MRRETLEFVD PFRPYQSFDT LKRFIQYDGK VLRFFCLWDD ST SLFGDRR EFVLHYFLCD GTVEIREVLP SNSGRDAMSS FLRRGKLPKY GPPGIYQPGQ ITDRAVLNVY GGLSEWRADG YLL DKYQLG KVEQDFYTDQ DLSIGATINV WGRKVLLCDC DEFTKTYYRT KYGVDNFTPI SCKPPHLPKI ERKYPPYTGF GSEE DSFRS CVGLKPTPHR KNFKKFMELD SFGNISNILR YFGKLITHKC ADVDRIFVIA FYLSDDTISV FEPIENNSGN AGGMF LKRS RVKKPGQEVF KSEFSEYIKA EELYIGATVN INGYLFILLN ADEYTLNYME NNTDKFPYSN FELAIQKLKQ EKSKSR EIT QVFAAADYNH TKVVPYNTFR DILMSITMGK LIDQELITIA RHYRVPEIMD PDLAYLIARA HEKFKKNIFE NFDMFIY NC VYEDREKKGV LPTKDIRRMC KSSRLPLDDD FLDCLLSRFE DKDHQINYEI FFSVLNWRMN PTPDLQAPPY LKEKCEDV W VGMPSPIPVK YVRYLDFLID VYGLEDNML

UniProtKB: EF-hand domain-containing family member C2

Macromolecule #32: Spermatid-specific manchette-related protein 1

• Macromolecule: Name: Spermatid-specific manchette-related protein 1 / type: protein_or_peptide / ID: 32 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.977191 KDa

Sequence

String:

MFLFSRKTKT PISTYSDSYR APTSIKEVYK DPPLWAWEAN KFVTPGLTQT MHRHVDPEAL QKMTKCAAQD YTYKSSISGH PYLPEKYWL SPDEEDKCCP SYLDNDRYNT WKTSPCSNYW NKYTGCLPRL SKDTGMESVR GMPLEYPPKQ ERLNAYEREV V VNMLNSLS RNRTLPQIVP RCGCVDPLPG RLPYQGYESP CSGRHYCLRG MDYCTTREPS TERRLRPLCS QQPTECVALR SP ARNAMCC YNSPAIILPV SQP

UniProtKB: Sperm microtubule inner protein 6

Macromolecule #33: Protein phosphatase 1 regulatory subunit 32

• Macromolecule: Name: Protein phosphatase 1 regulatory subunit 32 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 47.873078 KDa

Sequence

String:

MMGKLPLGVV SPYVKMSSGG CSDPLKFYAT SYCTAYGREE FKPRLGSHVG TGYKSNYRPL VSYQPHLDTL DNPAIGQQIR DTSKSVTSQ SYSPLEVPDG KQPLPWNLHQ TTSSYGREKL NPGPHSKEVR KVHFDTQDHG PQTITGLEPK EVPLIHQQQG K GSTEWENS HYGPRFMTSE YNSKYIKESP NHPDLLLKKT IGSKEETGFT EESTKNPIVF QPPSQAFPGD PVLHPGRSIT KS DYLPVTH PQGSDFLPVL SRGSDRDTGF SRVNERTLNP RVPTPAPQPA SMSHRSYQPP QRMQQTNVAL LGRESVGNKE PTG FTLNNP SYVRSSYEQD RDQRYLTTYN QGYFENIPKG LDREGWTRGG IQPQKAGAYA LSELNNHTLM DSTPNPTETL RHLH PHVGR TLASVDPFYR DMPHSSRYPA SS

UniProtKB: Stabilizer of axonemal microtubules 4

Macromolecule #34: Testis-specific serine/threonine-protein kinase 6

• Macromolecule: Name: Testis-specific serine/threonine-protein kinase 6 / type: protein_or_peptide / ID: 34 / Number of copies: 3 / Enantiomer: LEVO / EC number: non-specific serine/threonine protein kinase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 30.332943 KDa

Sequence

String:

MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP DFVNKFLPRE LSILRGVRHP HIVHVFEFIE VCNGKLYIV MEAAATDLLQ AVQRNGRIPG SQARELFSQI AGAVRYLHDH HLVHRDLKCE NVLLSPDERR VKITDFGFGR Q AHGYPDLS TTYCGSAAYA SPEVLLGIPY DPKKYDVWSL GVVLYVMVTG CMPFDDSDIA GLPRRQKRGV LYPDGLELSE RC KSLIAEL LQFSPSARPS AGQVARNGWL RAGDSG

UniProtKB: Testis-specific serine/threonine-protein kinase 6

Macromolecule #35: Cilia- and flagella-associated protein 20

• Macromolecule: Name: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 35 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.781389 KDa

Sequence

String:

MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

UniProtKB: Cilia- and flagella-associated protein 20

Macromolecule #36: Outer dense fiber protein 3

• Macromolecule: Name: Outer dense fiber protein 3 / type: protein_or_peptide / ID: 36 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 27.690973 KDa

Sequence

String:

MAEEVWMGTW RPHRPRGPIM ALYSSPGPKY LIPPTTGFVK HTPTKLRAPA YSFRGAPMLL AENCSPGPRY SVNPKILKTG KDLGPAYSI LGRYHTKTLL TPGPGDYFPE KSTKYVFDSA PSHSISARTK TFRVDSTPGP AAYMLPVVMG PHTVGKVSQP S FSIKGRSK LGSFSDDLHK TPGPAAYRQT EVQVTKFKAP QYTMAARVEP PGDKTLKPGP GAHSPEKVTL NKPCAPTVTF GI KHSDYMT PLVVDVE

UniProtKB: Ciliary microtubule associated protein 1A

Macromolecule #37: Parkin coregulated gene protein homolog

• Macromolecule: Name: Parkin coregulated gene protein homolog / type: protein_or_peptide / ID: 37 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 27.763268 KDa

Sequence

String:

MPKRTKLLPQ QTFQVHQPRS LVSEGFTVKA MMKNSVVRGP PVAGAFKERP AKPTTFRKCY ERGDFPIALE HDSKGNKIAW KVEIEKLDY HHYLPLFFDG LSEMTFPYEF FARRGIHDML EHGGNKILPV IPQLIIPIKN ALNLRNRQII CVTLKVLQHL V VSSEMVGE ALLPYYRQIL PILNIFKNMN VNSGDGIDYS QQKRENIGDL IQETLEAFER YGGEDAFINI KYMVPTYESC LL N

UniProtKB: Parkin coregulated gene protein homolog

Macromolecule #38: Testis, prostate and placenta-expressed protein

• Macromolecule: Name: Testis, prostate and placenta-expressed protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.985697 KDa

Sequence

String:

MAQIIDLVPW DECSAHLYAS PAVLLPLERV RHPLAGVKHQ LYHPALPSLR RMDMDTVKGC LSDEHCQSST YFSKDDFNKA HFTLLGVPN KPLQCLDFTA TGQKLCHKYR GGKMIPIAPG INRVDWPCFT RAIEDWSKFV SRSEEFKLPC ANKRVEGFSG Y AVRYLKPE VTQNWRYCLN QNPSLDRYGQ KPLPFDSLNA FRRFGSHYSR INYLTPWH

UniProtKB: Sperm microtubule inner protein 8

Macromolecule #39: Cilia- and flagella-associated protein 45

• Macromolecule: Name: Cilia- and flagella-associated protein 45 / type: protein_or_peptide / ID: 39 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 65.962016 KDa

Sequence

String:

MPLRPGDASS SASTASNRSR TRTRYRTKAM NSEVDESLFG GVKPSSQGKS DSPIVVIHDK HAIRKTLSAL GLEHKTETIQ LITRDMVRE LIVPTEDPSG ESLIISPEEF ERIKWASQVL TKEELNAREQ ALKKEKEGIL EAVTIRKKIM KQKEMTWNNN K KLSDLEEV ARERAQNLLQ RADKLRMEQE EELKDMSKII LNAKCHAIRD AQILEKQQIQ KELDEEERRL DHMMEIDRRE SL QRQEDRE RKRREERVRG KRHIVEQIKK NEEERSLQAE HREQEKEQML AYLDRLQEED LQDLERRHQE KLKMQAEIKR IND ENQRQK AEMLAQERLA DQMVMEFTKK KMAREAEYEA EQEKIRREKE KEIARLRALQ EKAQDYQAEQ DALRAKRNQE VADR EWRRK EKENAQKKIE TEEKLRKSRL EQVAFKEHTL AVQVQRDRDE FERILRAQRE QIEREKQEQE KKAKGCLQHA NELRR QVRE NQQKHVQNRL ATFEEGRRLK EEAEKRRERI EDIKKQKLEE LRATGLPEKY CIEVERKANI LPATSVN

UniProtKB: Cilia- and flagella-associated protein 45

Macromolecule #40: Uncharacterized protein C10orf82 homolog

• Macromolecule: Name: Uncharacterized protein C10orf82 homolog / type: protein_or_peptide / ID: 40 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 25.847469 KDa

Sequence

String:

MESPKTFMRK LPITPGYCGF IPWLSCQESS SEDRMNPCVK AFQERTQRYK EDQQGLNCSV ANTPPLKPIC SEDTVLWVLH EYAKKYHPL TLECKNEKKP LQEPPIPGWA GYLPRARVTE FGYATRYTIM AKKCYKDFLD LVEQAKRAQL KPYEQTYDVR A AQPLSPSS KILQLQGLSP AFPEFSGPGQ TPPSEDPQAP RPCGCAQWSS QSCSRNVYGE PPSLAKAFAE S

UniProtKB: Sperm-associated microtubule inner protein 5

Macromolecule #41: Coiled-coil domain-containing protein 105

• Macromolecule: Name: Coiled-coil domain-containing protein 105 / type: protein_or_peptide / ID: 41 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 57.406484 KDa

Sequence

String:

MPVLLPSTDP GQDSRVGAPE WRQAAKATSR KAHLLTDRCG QEAVTMWQPK DSVLDPNVAH HLGRAAYMEP WRFRVEMLKG GGTLEKPPP GEGVTLWKGK MKPPAWYARL PLPMHRDARA QQTAEVVHAH ARGARLTAAR LGRAQHQING QLRLLLRQRE A TDRRLSEV RKGLLINQQS VKLRGYRPKC EKIPDKADSL LVWEKKELKS MKRKMEKDME ISEDLLKALA SCRDTLDFYC QE RLQAVEL MNQPLDKVLE QAGRHSWVDI TRPPTPRTQG LKTPPPDPIG TYTPACAKAL FEAKRLLMES KDILTEMAKN EVD IQNQQQ EISNRVCSSL AQKMRETLEL KERMTMTLGL MRGTIHRCMK FNQEMYVTRG IIKGPLLKRN LEAREKLNRP LVRM YQRHV GTQLPEATRL AQGTDLLTRH NLHMEKNLKE LRTTHDNLAW GLNCKKIGHD VDYDVVRLRL RQRHPHVCYE QAQRL VNDW DPPTPARSQT NTASK

UniProtKB: Tektin-like protein 1

Macromolecule #42: Cilia- and flagella-associated protein 52

• Macromolecule: Name: Cilia- and flagella-associated protein 52 / type: protein_or_peptide / ID: 42 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 68.322164 KDa

Sequence

String:

MEEQVLPELD VAELELQAVI GFNGHVPNGL KCHPDQEHLI YPLGCTVLIQ AINTNEQNFL HGHGNNVSCV TISKEGDYIA SGQVTFMGF KADIILWDFK KRELIARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKRDAIC GSPAAGLNVG N ATSVVFSR CRDEMFVTAG NGTIRVWELD LPNRKIWPTE CQTGQMKRIV LSTGMADDDS FFYLGTTTGD ILKMNPKTKL LA DTGPVKD KFSLGVSALR CLKMGGLLVG SGAGLLIFCK SPSYKPIKKV QLQGGITSIT LRGEGHQFFV GTEESHIYRV NFT DFKETL IATCHFEAVQ DIVFPFGTAE LFATCAKKDI RVWHTMSKRE LLRITVPNMT CHGIDFMRDG KSIISAWDDG KIRA FAPES GRLMYTINSA HRIGVTAIAT TSDCKRIISG GGEGEVRVWQ VGCQTQKLEE ALKEHKSSVS CIRVKKNNEE CVTAS TDGT CIIWDLVRLR RNQMILANTL FQCVCYHPEE FQIITSGTDR KIAYWEVFDG SVIRELEGSL SGSINGMDIT QEGGHF VTG GHDHLVKVWD YNEGEVTHVG VGHSGNIMAM RISPGNQYIV SVSADGAILR WKYPFAS

UniProtKB: Cilia- and flagella-associated protein 52

Macromolecule #43: Enkurin

• Macromolecule: Name: Enkurin / type: protein_or_peptide / ID: 43 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.587521 KDa

Sequence

String:

MDSPCTSESI YNLIPSDLKE PPQHPRYTSL FRATIKNDMK KFKTAMKTMG PAKVEIPSPK DFLKKHSKEK TLPPKKKFNR CSPKKPAVP LRTDHPVMGI QSGKNFINTN AADVIMGVAK KPKPIYVDKR TGDKHDLETS GLFPKYINKK DYGITPEYIC K RNEDVKKA QEEYDNYIQE NLKKAAMKRL SDEEREAVLQ GLKKNWEEVH KEFQSLSVFI DSVPKKIRKQ KLEKEMKQLE HD ISVIEKH KIIYIANK

UniProtKB: Enkurin

Macromolecule #44: Testis-expressed protein 43

• Macromolecule: Name: Testis-expressed protein 43 / type: protein_or_peptide / ID: 44 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.305608 KDa

Sequence

String:

MASEKDDGPA LPKLDDDNQT AENTCKPAEE QPQQLRWDDI HLPRFSLKQG MIPTRYVMPW KENMKFRNVN LQQAEACGIY AGPLEDSLF WGYSERLCHG EDRKAVLKKG LPEIKITDMP LHSPLSRYQS TVISHGFRRR LI

UniProtKB: Sperm-associated microtubule inner protein 10

Macromolecule #45: Protein Flattop

• Macromolecule: Name: Protein Flattop / type: protein_or_peptide / ID: 45 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.575123 KDa

Sequence

String:

MATNYSANQY EKAYLPTYLQ NWSPARPTKE KIAAHEGYTQ IIANDRGHLL PSVPRSKASP WGSFMGTWQM PLKIPPAKVT LTARTTTAA DNLTKWIHKN PDLLNACNGL RPEISGKPFD PDSQTKQKKS VTKTVQQAPN PTIIPSSPVI QGDNPDEPQS S HPSAGHTP GPQTPVNSPN NPPPSPCKST K

UniProtKB: Protein Flattop

Macromolecule #46: EF-hand calcium-binding domain-containing protein 6

• Macromolecule: Name: EF-hand calcium-binding domain-containing protein 6 / type: protein_or_peptide / ID: 46 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 176.028312 KDa

Sequence

String:

MKRNGTRLNF AKANSTKSGS TRARDLMNKM SIIPEWHSLY SNTRKLPCSR PHSSPCKMQR TIFQDLCRPS SSTTAIANPV LSYLDIERI LAQKISSRRD DIKKVFQILD RNHNQMVTKG DLKRVITAFL IPLTKDQFQD LLAQIPISSL GNVPYLEFLS R FGGGIDIN INGIKRVNEN EVDNRRTVKE VQLTEKIFRN MRSIRKVFQV MDVNNTGLVQ PQELRRVLET FCLRMQDGDY EK FLEQYNI DKTTAVDYNA FLKNLSVKND ASFKYLLSNA AELSRETQQG KNGKRLLDTG SSEDVWKNYS LDDLEKTFCQ EFS KSYEKI EKALSAGDPS KGGYISLNYL KVVLDTFIYR LPRRIFIQLI RRFGLKTSTK INWKQFLTAI YERQKLEVSK TLPL KKRSS TEARNRSRKE NIIKKLFKYS EDRYTALKKT LLIISSTPSG HITWEELRHI LNCMVAKLND SEFSELKQTF DPEGT GAVR VNSLLDVLDD STKVRKMSPS TDTKTPLPVA WDSVEELVLD SITRNLQAFY SMLQSYDLRD TGTIGKNNFR KVMRVF CPY LSNEHLVRFS SKFQEAGSGR ILYKKFLLSI GVGIPPPTPP LSSPKVQLSD QFQIEEPGQQ DERTQPSGEK TSEINNM TK EEVIDGLKHR IQQKDPVFRK QFLSISKEPD VKINQEEFRK VLERSGMPMN DCQYAMLASK LGFKNEEGMS YQDFTMGF E DCMLSGLETV PLQSRTASRT NMDEHFISAE ECLRIFPKKL KESFRDVYSA FFRIDLDRDG IISMHDFHRL LQYLQLNMV DLEFERFLSL LGLRLSVTLN FREFQNLCEK RPWKSDEAPQ RLIRCKQKVA DSELACEQAH QYLIMKAKTR WADLSKNFIE TDNEGNGIL RRRDIKNSLY GFDIPLTPRE FEKLWQNYDT EGRGYITYQE FLHRLGIRYS PKVHRPYKED YFNFLGHFTK P KQVQEEIQ ELQQISEREK LMNHYEEISK AFNAMEKSKP VALCRVQKVL QECGCPLKEE ELISLLKSLD VSVHNNHIDP VE FLRALEI SWASKARPKE KEESSPPPIS FSKVTPDEVI KTMQEVVESS QPALVEAFSA LDKEDTGFVK AMEFGDVLRS VCQ KLTDNQ YHYFLRRLRL HLTPNIHWKY FLENFSTFQD ETADDWAENM PKAPPPMSPK ETAHRDIVAR VQKAVASHYH TIVQ EFENF DTLKSNTVSR DEFRSICTRH IQILTDEQFD RLWSELPVNA KGRLKYQDFL SKLSIERVPS PPMAAGDSGE STMAQ RGSS APEFSQGTRS NLYSPPRDSR VGLKSRSHPC TPVGTPPLQN CEPIESRLRK QIQGCWRELL RECKEKDTDK QGTISA AEF LALVEKFKLD ISREESQQLI VKYDLKNNGK FAYCDFIQSC VLLLKAKETS LMRRMRIQNA DKMKEAGMET PSFYSAL LR IQPKIVHCWR PMRRSFKTYD KNGTGLLSVA DFRKVLRQYS INLSEEEFFH VLEYYDKSLS SKISYNDFLR AFLQ

UniProtKB: EF-hand calcium-binding domain-containing protein 6

Macromolecule #47: Cilia- and flagella-associated protein 210

• Macromolecule: Name: Cilia- and flagella-associated protein 210 / type: protein_or_peptide / ID: 47 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 65.26652 KDa

Sequence

String:

MQVRFGRRSG QAKESQGMDC FEKEEIPYPP LLPSKVDLQQ ITIIPHAEWE RIRDSLNRLT REAAVLRAER AAKKKMHVKS QELVKHWTN TYAGMKEQKL KAKQKRDEEI EAERKVLDLE EEIYKEGERK KAIESARQCQ FHQTERVKRF HSGLLLSRVM K ERDVQIQY KKNAVKSDKK WEEQVKLNDE KAFKEDQEKE EKRRRERVAL AEDHLKQIEE HKEEEEARKK SEEKDAEEMK RQ NLLYEIE MKKNLSKKQE EIDTNRKLLL DNMHNKNIIR AVEQQQQEEE DEKIRKFIKA KKRLIQMRMD KDAETHRLME ERR ERINNF LSKLIKEKLD TEDLIIARDI SEADAELEKR EKEKHEKNQA DLKAIAEYRA SVMKNKEEEE RQRKIEAKEQ LQAV LKADK IFQELEKEKS LKVTREKLEI QDAHIQQIAI NKYNAKQMKE EELDYWRLTD ALTVEKEKEF EKYAREVINF ESEST KKYA YPMVKAVQEG VGGGRGPPFV GRGGIRPSYQ ATDATGVQLP CFKSQGSKYN DFQKSKRRLG FTW

UniProtKB: Cilia- and flagella- associated protein 210

Macromolecule #48: Cilia- and flagella-associated protein 276

• Macromolecule: Name: Cilia- and flagella-associated protein 276 / type: protein_or_peptide / ID: 48 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.960092 KDa

Sequence

String:

MLQPQETFSN PALRDEDKHL GNLWASKKSL YKNPAHLAQQ QDPWSRLSST PTATSRSRDT FFDSKIPKDD LDFRLATLYN HHTGAFKNK TEILLHQETI EDIQGTKIQF PGECFHAPSA PITSRTTIRH WINPKKESIH SIQGSIVSPH TAATNGGYSR K NDGGFFST

UniProtKB: Cilia- and flagella-associated protein 276

Macromolecule #49: Piercer of microtubule wall 1 protein

• Macromolecule: Name: Piercer of microtubule wall 1 protein / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.862852 KDa

Sequence

String:

MSEEKPQQSA EEPEPGEPKA KPAPEEPEPG EPKAKPAPEE PEPGEPKAKP APEKTSDYYR ISEKLPVRFN NPGWFHGYGT KEAVSMYRT SNQTYGSRAP TVHEMPKVFY PSSNKFSRQH AAFGMFQSHN INVTLEKSLV TGPDNHITHY DRLNFHPSYN V NRPSICD

UniProtKB: Piercer of microtubule wall 1 protein

Macromolecule #50: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 50 / Number of copies: 178 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #51: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 51 / Number of copies: 178 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #52: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 52 / Number of copies: 176 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number real images: 25135 / Average exposure time: 2.56 sec. / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.6 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 81000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

24664

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 95290
• Initial angle assignment: Type: NOT APPLICABLE
• Final angle assignment: Type: NOT APPLICABLE