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- PDB-8iyj: Cryo-EM structure of the 48-nm repeat doublet microtubule from mo... -

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Basic information

Entry
Database: PDB / ID: 8iyj
TitleCryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm
Components
  • (Cilia- and flagella-associated protein ...) x 12
  • (EF-hand domain-containing family member ...) x 2
  • (Piercer of microtubule wall ...) x 2
  • (RIB43A-like with coiled-coils protein ...) x 2
  • (Testis-expressed ...) x 2
  • Cilia and flagella-associated protein 77
  • Coiled-coil domain-containing protein 105
  • Dual specificity phosphatase 21
  • EF-hand calcium-binding domain-containing protein 6
  • EF-hand domain-containing protein 1
  • Enkurin
  • Family with sequence similarity 183, member B
  • Meiosis-specific nuclear structural protein 1
  • Nucleoside diphosphate kinase 7
  • Outer dense fiber protein 3
  • Parkin coregulated gene protein homolog
  • Protein FAM166A
  • Protein FAM166C
  • Protein Flattop
  • Protein phosphatase 1 regulatory subunit 32
  • Sperm-associated antigen 8
  • Spermatid-specific manchette-related protein 1
  • Tektin bundle-interacting protein 1
  • Tektin-1
  • Tektin-2
  • Tektin-3
  • Tektin-4
  • Tektin-5
  • Testis expressed 49
  • Testis, prostate and placenta-expressed protein
  • Testis-specific serine/threonine-protein kinase 6
  • Tubulin alpha-3 chain
  • Tubulin beta-4B chain
  • Uncharacterized protein C10orf82 homolog
KeywordsSTRUCTURAL PROTEIN / doublet microtubule
Function / homology
Function and homology information


male germ-line stem cell population maintenance / protein localization to motile cilium / left/right pattern formation / axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane ...male germ-line stem cell population maintenance / protein localization to motile cilium / left/right pattern formation / axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / outer dense fiber / Carboxyterminal post-translational modifications of tubulin / Intraflagellar transport / protein polyglutamylation / inner dynein arm assembly / regulation of calcineurin-NFAT signaling cascade / sperm axoneme assembly / regulation of microtubule nucleation / positive regulation of feeding behavior / COPI-independent Golgi-to-ER retrograde traffic / Transferases; Transferring phosphorus-containing groups / sperm principal piece / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / regulation of cilium beat frequency involved in ciliary motility / MAP kinase tyrosine/serine/threonine phosphatase activity / cerebrospinal fluid circulation / epithelial cilium movement involved in extracellular fluid movement / cilium movement involved in cell motility / COPI-mediated anterograde transport / Aggrephagy / 9+2 motile cilium / Kinesins / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / protein localization to organelle / regulation of store-operated calcium entry / intraciliary transport / Resolution of Sister Chromatid Cohesion / PKR-mediated signaling / acrosomal membrane / The role of GTSE1 in G2/M progression after G2 checkpoint / axoneme assembly / RHO GTPases activate IQGAPs / microtubule sliding / Recycling pathway of L1 / cilium movement / ciliary transition zone / left/right axis specification / calcium ion sensor activity / axonemal microtubule / COPI-dependent Golgi-to-ER retrograde traffic / sperm DNA condensation / RHO GTPases Activate Formins / Separation of Sister Chromatids / cilium organization / Hedgehog 'off' state / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / gamma-tubulin ring complex / Regulation of PLK1 Activity at G2/M Transition / manchette / positive regulation of cilium assembly / flagellated sperm motility / MHC class II antigen presentation / cell projection organization / UTP biosynthetic process / CTP biosynthetic process / determination of left/right symmetry / intermediate filament / protein targeting to membrane / extrinsic component of membrane / positive regulation of cell motility / nucleoside diphosphate kinase activity / protein targeting to mitochondrion / GTP biosynthetic process / tubulin complex / AMP binding / protein-serine/threonine phosphatase / ciliary base / receptor clustering / protein serine/threonine phosphatase activity / phosphatase activity / regulation of cell division / microtubule organizing center / phosphoprotein phosphatase activity / mitotic cytokinesis / cellular response to UV-C / axoneme / cilium assembly / glial cell projection
Similarity search - Function
Spermatid-specific manchette-related protein 1 / Testis-expressed sequence 37 protein / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / : / Spermatid-specific manchette-related protein 1 / TSC21 family / Sperm-associated microtubule inner protein 5 / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein ...Spermatid-specific manchette-related protein 1 / Testis-expressed sequence 37 protein / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / : / Spermatid-specific manchette-related protein 1 / TSC21 family / Sperm-associated microtubule inner protein 5 / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Sperm microtubule inner protein 8 / Sperm microtubule inner protein 11 / : / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / : / Cilia- and flagella-associated protein 68 / Sperm-associated microtubule inner protein 10 / Protein of unknown function DUF4541 / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Tektin bundle interacting protein 1 / Cilia and flagella associated protein 90 / : / Sperm-tail PG-rich repeat / DJBP, EF-hand domain / FAM183 family / Sperm-tail PG-rich repeat / EF-hand domain / FAM183A and FAM183B related / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Protein CFAP95 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / : / Tektin family / Protein of unknown function (DUF3695) / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 161-like domain / : / EFHB C-terminal EF-hand domain / : / Nucleoside diphosphate kinase 7 N-terminal PH domain / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dual specificity phosphatase, catalytic domain / EF hand / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity protein phosphatase domain profile. / Dual specificity protein phosphatase domain / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase (NDPK)-like domain profile. / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / EF-hand domain pair / Mir domain superfamily
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Cilia- and flagella-associated protein 77 / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Cilia- and flagella-associated protein 144 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Stabilizer of axonemal microtubules 4 ...GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Cilia- and flagella-associated protein 77 / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Cilia- and flagella-associated protein 144 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Stabilizer of axonemal microtubules 4 / Tektin-4 / Sperm microtubule inner protein 6 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 107 / Piercer of microtubule wall 1 protein / Cilia- and flagella-associated protein 52 / Meiosis-specific nuclear structural protein 1 / Sperm microtubule inner protein 8 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Cilia- and flagella-associated protein 161 / Enkurin / Tektin-3 / Cilia- and flagella-associated protein 20 / Sperm microtubule inner protein 11 / EF-hand domain-containing family member B / Ciliary microtubule associated protein 1A / Tektin-2 / Testis-specific serine/threonine-protein kinase 6 / Cilia- and flagella-associated protein 95 / Sperm-associated microtubule inner protein 5 / RIB43A-like with coiled-coils protein 1 / Cilia- and flagella-associated protein 68 / Cilia- and flagella-associated protein 53 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 2A / Tektin-like protein 1 / RIB43A-like with coiled-coils protein 2 / Dual specificity phosphatase 21 / Cilia- and flagella-associated protein 141 / Sperm-associated microtubule inner protein 10 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 45 / Cilia- and flagella-associated protein 276 / Protein SPMIP9 / Tektin-1 / Parkin coregulated gene protein homolog / Cilia- and flagella-associated protein 90 / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase homolog 7 / Piercer of microtubule wall 2 protein
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsZhou, L.N. / Gui, M. / Wu, J.P.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Cell / Year: 2023
Title: Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility.
Authors: Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu ...Authors: Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu / Miao Gui / Jianping Wu /
Abstract: Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron ...Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility.
History
DepositionApr 5, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 5, 2023Provider: repository / Type: Initial release
Revision 1.1Jul 3, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond / em_admin / Item: _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
0: Cilia- and flagella-associated protein 95
1: EF-hand domain-containing family member B
2: EF-hand domain-containing family member B
3: Cilia- and flagella-associated protein 53
4: Cilia- and flagella-associated protein 53
5: Nucleoside diphosphate kinase 7
6: Nucleoside diphosphate kinase 7
7: Cilia- and flagella-associated protein 95
8: Cilia- and flagella-associated protein 107
A: Cilia- and flagella-associated protein 141
A0: Tektin-1
A1: Tektin-1
A2: Tektin-1
A3: Tektin-1
A4: Tektin-1
A5: Tektin-1
AA: Tubulin alpha-3 chain
AB: Tubulin beta-4B chain
AC: Tubulin alpha-3 chain
AD: Tubulin beta-4B chain
AE: Tubulin alpha-3 chain
AF: Tubulin beta-4B chain
AG: Tubulin alpha-3 chain
AH: Tubulin beta-4B chain
AI: Tubulin alpha-3 chain
AJ: Tubulin beta-4B chain
AK: Tubulin alpha-3 chain
AL: Tubulin beta-4B chain
AM: Tubulin alpha-3 chain
AN: Tubulin beta-4B chain
AO: Tubulin alpha-3 chain
AP: Tubulin beta-4B chain
B: Meiosis-specific nuclear structural protein 1
B0: Tektin-2
B1: Tektin-2
B2: Tektin-2
B3: Tektin-2
B4: Tektin-2
B5: Tektin-2
BB: Tubulin beta-4B chain
BC: Tubulin alpha-3 chain
BD: Tubulin beta-4B chain
BE: Tubulin alpha-3 chain
BF: Tubulin beta-4B chain
BG: Tubulin alpha-3 chain
BH: Tubulin beta-4B chain
BI: Tubulin alpha-3 chain
BJ: Tubulin beta-4B chain
BK: Tubulin alpha-3 chain
BL: Tubulin beta-4B chain
BM: Tubulin alpha-3 chain
BN: Tubulin beta-4B chain
BO: Tubulin alpha-3 chain
BP: Tubulin beta-4B chain
C: Meiosis-specific nuclear structural protein 1
C0: Tektin-3
C1: Tektin-3
C2: Tektin-3
C3: Tektin-3
C4: Tektin-3
C5: Tektin-3
C6: Tektin-3
CB: Tubulin beta-4B chain
CC: Tubulin alpha-3 chain
CD: Tubulin beta-4B chain
CE: Tubulin alpha-3 chain
CF: Tubulin beta-4B chain
CG: Tubulin alpha-3 chain
CH: Tubulin beta-4B chain
CI: Tubulin alpha-3 chain
CJ: Tubulin beta-4B chain
CK: Tubulin alpha-3 chain
CL: Tubulin beta-4B chain
CM: Tubulin alpha-3 chain
CN: Tubulin beta-4B chain
CO: Tubulin alpha-3 chain
CP: Tubulin beta-4B chain
CQ: Tubulin alpha-3 chain
D: Sperm-associated antigen 8
D0: Tektin-4
D1: Tektin-4
D2: Tektin-4
D3: Tektin-4
D4: Tektin-4
D5: Tektin-4
DB: Tubulin beta-4B chain
DC: Tubulin alpha-3 chain
DD: Tubulin beta-4B chain
DE: Tubulin alpha-3 chain
DF: Tubulin beta-4B chain
DG: Tubulin alpha-3 chain
DH: Tubulin beta-4B chain
DI: Tubulin alpha-3 chain
DJ: Tubulin beta-4B chain
DK: Tubulin alpha-3 chain
DL: Tubulin beta-4B chain
DM: Tubulin alpha-3 chain
DN: Tubulin beta-4B chain
DO: Tubulin alpha-3 chain
DP: Tubulin beta-4B chain
DQ: Tubulin alpha-3 chain
E: Cilia- and flagella-associated protein 161
EA: Tubulin alpha-3 chain
EB: Tubulin beta-4B chain
EC: Tubulin alpha-3 chain
ED: Tubulin beta-4B chain
EE: Tubulin alpha-3 chain
EF: Tubulin beta-4B chain
EG: Tubulin alpha-3 chain
EH: Tubulin beta-4B chain
EI: Tubulin alpha-3 chain
EJ: Tubulin beta-4B chain
EK: Tubulin alpha-3 chain
EL: Tubulin beta-4B chain
EM: Tubulin alpha-3 chain
EN: Tubulin beta-4B chain
EO: Tubulin alpha-3 chain
F: Cilia- and flagella-associated protein 161
FA: Tubulin alpha-3 chain
FB: Tubulin beta-4B chain
FC: Tubulin alpha-3 chain
FD: Tubulin beta-4B chain
FE: Tubulin alpha-3 chain
FF: Tubulin beta-4B chain
FG: Tubulin alpha-3 chain
FH: Tubulin beta-4B chain
FI: Tubulin alpha-3 chain
FJ: Tubulin beta-4B chain
FK: Tubulin alpha-3 chain
FL: Tubulin beta-4B chain
FM: Tubulin alpha-3 chain
FN: Tubulin beta-4B chain
FO: Tubulin alpha-3 chain
G: Piercer of microtubule wall 2 protein
GA: Tubulin alpha-3 chain
GB: Tubulin beta-4B chain
GC: Tubulin alpha-3 chain
GD: Tubulin beta-4B chain
GE: Tubulin alpha-3 chain
GF: Tubulin beta-4B chain
GG: Tubulin alpha-3 chain
GH: Tubulin beta-4B chain
GI: Tubulin alpha-3 chain
GJ: Tubulin beta-4B chain
GK: Tubulin alpha-3 chain
GL: Tubulin beta-4B chain
GM: Tubulin alpha-3 chain
GN: Tubulin beta-4B chain
GO: Tubulin alpha-3 chain
H: Protein FAM166A
HA: Tubulin alpha-3 chain
HB: Tubulin beta-4B chain
HC: Tubulin alpha-3 chain
HD: Tubulin beta-4B chain
HE: Tubulin alpha-3 chain
HF: Tubulin beta-4B chain
HG: Tubulin alpha-3 chain
HH: Tubulin beta-4B chain
HI: Tubulin alpha-3 chain
HJ: Tubulin beta-4B chain
HK: Tubulin alpha-3 chain
HL: Tubulin beta-4B chain
HM: Tubulin alpha-3 chain
HN: Tubulin beta-4B chain
HO: Tubulin alpha-3 chain
HP: Tubulin beta-4B chain
I: Protein FAM166A
I1: Cilia- and flagella-associated protein 68
IA: Tubulin alpha-3 chain
IB: Tubulin beta-4B chain
IC: Tubulin alpha-3 chain
ID: Tubulin beta-4B chain
IE: Tubulin alpha-3 chain
IF: Tubulin beta-4B chain
IG: Tubulin alpha-3 chain
IH: Tubulin beta-4B chain
II: Tubulin alpha-3 chain
IJ: Tubulin beta-4B chain
IK: Tubulin alpha-3 chain
IL: Tubulin beta-4B chain
IM: Tubulin alpha-3 chain
IN: Tubulin beta-4B chain
IO: Tubulin alpha-3 chain
IP: Tubulin beta-4B chain
J: Protein FAM166A
J1: Cilia and flagella-associated protein 77
J2: Cilia and flagella-associated protein 77
J3: Cilia and flagella-associated protein 77
J4: Cilia and flagella-associated protein 77
J5: Cilia and flagella-associated protein 77
JB: Tubulin beta-4B chain
JC: Tubulin alpha-3 chain
JD: Tubulin beta-4B chain
JE: Tubulin alpha-3 chain
JF: Tubulin beta-4B chain
JG: Tubulin alpha-3 chain
JH: Tubulin beta-4B chain
JI: Tubulin alpha-3 chain
JJ: Tubulin beta-4B chain
JK: Tubulin alpha-3 chain
JL: Tubulin beta-4B chain
JM: Tubulin alpha-3 chain
JN: Tubulin beta-4B chain
JO: Tubulin alpha-3 chain
JP: Tubulin beta-4B chain
JQ: Tubulin alpha-3 chain
K: Protein FAM166A
K1: Family with sequence similarity 183, member B
K2: Family with sequence similarity 183, member B
K3: Sperm-associated antigen 8
KA: Tubulin alpha-3 chain
KB: Tubulin beta-4B chain
KC: Tubulin alpha-3 chain
KD: Tubulin beta-4B chain
KE: Tubulin alpha-3 chain
KF: Tubulin beta-4B chain
KG: Tubulin alpha-3 chain
KH: Tubulin beta-4B chain
KI: Tubulin alpha-3 chain
KJ: Tubulin beta-4B chain
KK: Tubulin alpha-3 chain
KL: Tubulin beta-4B chain
KM: Tubulin alpha-3 chain
KN: Tubulin beta-4B chain
KO: Tubulin alpha-3 chain
KP: Tubulin beta-4B chain
L: Protein FAM166A
L1: Cilia- and flagella-associated protein 90
LA: Tubulin alpha-3 chain
LB: Tubulin beta-4B chain
LC: Tubulin alpha-3 chain
LD: Tubulin beta-4B chain
LE: Tubulin alpha-3 chain
LF: Tubulin beta-4B chain
LG: Tubulin alpha-3 chain
LH: Tubulin beta-4B chain
LI: Tubulin alpha-3 chain
LJ: Tubulin beta-4B chain
LK: Tubulin alpha-3 chain
LL: Tubulin beta-4B chain
LM: Tubulin alpha-3 chain
LN: Tubulin beta-4B chain
LO: Tubulin alpha-3 chain
LP: Tubulin beta-4B chain
M: Protein FAM166A
M1: Protein FAM166C
M2: Protein FAM166C
M3: Protein FAM166C
M4: Protein FAM166C
MA: Tubulin alpha-3 chain
MB: Tubulin beta-4B chain
MC: Tubulin alpha-3 chain
MD: Tubulin beta-4B chain
ME: Tubulin alpha-3 chain
MF: Tubulin beta-4B chain
MG: Tubulin alpha-3 chain
MH: Tubulin beta-4B chain
MI: Tubulin alpha-3 chain
MJ: Tubulin beta-4B chain
MK: Tubulin alpha-3 chain
ML: Tubulin beta-4B chain
MM: Tubulin alpha-3 chain
MN: Tubulin beta-4B chain
MO: Tubulin alpha-3 chain
MP: Tubulin beta-4B chain
N: Protein FAM166A
N1: Cilia- and flagella-associated protein 141
N2: Cilia- and flagella-associated protein 161
NA: Tubulin alpha-3 chain
NB: Tubulin beta-4B chain
NC: Tubulin alpha-3 chain
ND: Tubulin beta-4B chain
NE: Tubulin alpha-3 chain
NF: Tubulin beta-4B chain
NG: Tubulin alpha-3 chain
NH: Tubulin beta-4B chain
NI: Tubulin alpha-3 chain
NJ: Tubulin beta-4B chain
NK: Tubulin alpha-3 chain
NL: Tubulin beta-4B chain
NM: Tubulin alpha-3 chain
NN: Tubulin beta-4B chain
NO: Tubulin alpha-3 chain
NP: Tubulin beta-4B chain
O: RIB43A-like with coiled-coils protein 1
O1: Dual specificity phosphatase 21
O2: Dual specificity phosphatase 21
O3: Dual specificity phosphatase 21
O4: Dual specificity phosphatase 21
O5: EF-hand domain-containing protein 1
O6: EF-hand domain-containing protein 1
O7: Protein FAM166A
O8: Protein FAM166A
O9: Protein FAM166A
OA: Tubulin alpha-3 chain
OB: Tubulin beta-4B chain
OC: Tubulin alpha-3 chain
OD: Tubulin beta-4B chain
OE: Tubulin alpha-3 chain
OF: Tubulin beta-4B chain
OG: Tubulin alpha-3 chain
OH: Tubulin beta-4B chain
OI: Tubulin alpha-3 chain
OJ: Tubulin beta-4B chain
OK: Tubulin alpha-3 chain
OL: Tubulin beta-4B chain
OM: Tubulin alpha-3 chain
ON: Tubulin beta-4B chain
OO: Tubulin alpha-3 chain
OP: Tubulin beta-4B chain
P: RIB43A-like with coiled-coils protein 1
P1: Testis expressed 49
P2: Testis expressed 49
P3: Testis-expressed sequence 37 protein
P4: Testis-expressed sequence 37 protein
P5: Testis-expressed sequence 37 protein
P6: Testis-expressed sequence 37 protein
P7: Testis-expressed sequence 37 protein
P8: Protein FAM166A
P9: Protein FAM166A
PB: Tubulin beta-4B chain
PC: Tubulin alpha-3 chain
PD: Tubulin beta-4B chain
PE: Tubulin alpha-3 chain
PF: Tubulin beta-4B chain
PG: Tubulin alpha-3 chain
PH: Tubulin beta-4B chain
PI: Tubulin alpha-3 chain
PJ: Tubulin beta-4B chain
PK: Tubulin alpha-3 chain
PL: Tubulin beta-4B chain
PM: Tubulin alpha-3 chain
PN: Tubulin beta-4B chain
PO: Tubulin alpha-3 chain
PP: Tubulin beta-4B chain
Q: RIB43A-like with coiled-coils protein 1
Q1: Tektin bundle-interacting protein 1
Q2: Tektin bundle-interacting protein 1
Q3: Tektin bundle-interacting protein 1
Q4: Tektin bundle-interacting protein 1
Q5: Tektin bundle-interacting protein 1
QB: Tubulin beta-4B chain
QC: Tubulin alpha-3 chain
QD: Tubulin beta-4B chain
QE: Tubulin alpha-3 chain
QF: Tubulin beta-4B chain
QG: Tubulin alpha-3 chain
QH: Tubulin beta-4B chain
QI: Tubulin alpha-3 chain
QJ: Tubulin beta-4B chain
QK: Tubulin alpha-3 chain
QL: Tubulin beta-4B chain
QM: Tubulin alpha-3 chain
QN: Tubulin beta-4B chain
QO: Tubulin alpha-3 chain
QP: Tubulin beta-4B chain
R: RIB43A-like with coiled-coils protein 2
R0: Tektin-2
R1: Tektin-2
R2: Tektin-2
R3: Tektin-2
R4: Tektin-2
R5: Tektin-2
RC: Tubulin alpha-3 chain
RD: Tubulin beta-4B chain
RE: Tubulin alpha-3 chain
RF: Tubulin beta-4B chain
RG: Tubulin alpha-3 chain
RH: Tubulin beta-4B chain
RI: Tubulin alpha-3 chain
RJ: Tubulin beta-4B chain
RK: Tubulin alpha-3 chain
RL: Tubulin beta-4B chain
RM: Tubulin alpha-3 chain
RN: Tubulin beta-4B chain
RO: Tubulin alpha-3 chain
RP: Tubulin beta-4B chain
S: RIB43A-like with coiled-coils protein 2
S0: Tektin-3
S1: Tektin-3
S2: Tektin-3
S3: Tektin-3
S4: Tektin-3
S5: Tektin-3
S6: Tektin-3
S7: Tektin-3
S8: Tektin-3
S9: Tektin-3
SA: Tubulin alpha-3 chain
SB: Tubulin beta-4B chain
SC: Tubulin alpha-3 chain
SD: Tubulin beta-4B chain
SE: Tubulin alpha-3 chain
SF: Tubulin beta-4B chain
SG: Tubulin alpha-3 chain
SH: Tubulin beta-4B chain
SI: Tubulin alpha-3 chain
SJ: Tubulin beta-4B chain
SK: Tubulin alpha-3 chain
SL: Tubulin beta-4B chain
SM: Tubulin alpha-3 chain
SN: Tubulin beta-4B chain
T: EF-hand domain-containing protein 1
T0: Tektin-4
T1: Tektin-4
T2: Tektin-4
T3: Tektin-4
T4: Tektin-4
T5: Tektin-4
TA: Tubulin alpha-3 chain
TB: Tubulin beta-4B chain
TC: Tubulin alpha-3 chain
TD: Tubulin beta-4B chain
TE: Tubulin alpha-3 chain
TF: Tubulin beta-4B chain
TG: Tubulin alpha-3 chain
TH: Tubulin beta-4B chain
TI: Tubulin alpha-3 chain
TJ: Tubulin beta-4B chain
TK: Tubulin alpha-3 chain
TL: Tubulin beta-4B chain
TM: Tubulin alpha-3 chain
TN: Tubulin beta-4B chain
U: EF-hand domain-containing protein 1
U0: Tektin-5
U1: Tektin-5
U2: Tektin-5
U3: Tektin-5
U4: Tektin-5
U5: Tektin-5
U6: Tektin-5
U7: Tektin-5
U8: Tektin-5
U9: Tektin-5
UA: Tubulin alpha-3 chain
UB: Tubulin beta-4B chain
UC: Tubulin alpha-3 chain
UD: Tubulin beta-4B chain
UE: Tubulin alpha-3 chain
UF: Tubulin beta-4B chain
UG: Tubulin alpha-3 chain
UH: Tubulin beta-4B chain
UI: Tubulin alpha-3 chain
UJ: Tubulin beta-4B chain
UK: Tubulin alpha-3 chain
UL: Tubulin beta-4B chain
UM: Tubulin alpha-3 chain
UN: Tubulin beta-4B chain
UO: Tubulin alpha-3 chain
V: EF-hand domain-containing protein 1
V0: Tektin-5
V1: Tektin-5
V2: Tektin-5
V3: Tektin-5
V4: Tektin-5
V5: Tektin-5
V6: Tektin-5
V7: Tektin-5
V8: Tektin-5
V9: Tektin-5
VA: Tubulin alpha-3 chain
VB: Tubulin beta-4B chain
VC: Tubulin alpha-3 chain
VD: Tubulin beta-4B chain
VE: Tubulin alpha-3 chain
VF: Tubulin beta-4B chain
VG: Tubulin alpha-3 chain
VH: Tubulin beta-4B chain
VI: Tubulin alpha-3 chain
VJ: Tubulin beta-4B chain
VK: Tubulin alpha-3 chain
VL: Tubulin beta-4B chain
VM: Tubulin alpha-3 chain
VN: Tubulin beta-4B chain
VO: Tubulin alpha-3 chain
W: EF-hand domain-containing family member C2
W0: Tektin-5
W2: Tektin-5
W4: Tektin-5
W5: Tektin-5
W6: Tektin-5
W7: Tektin-5
WA: Tubulin alpha-3 chain
WB: Tubulin beta-4B chain
WC: Tubulin alpha-3 chain
WD: Tubulin beta-4B chain
WE: Tubulin alpha-3 chain
WF: Tubulin beta-4B chain
WG: Tubulin alpha-3 chain
WH: Tubulin beta-4B chain
WI: Tubulin alpha-3 chain
WJ: Tubulin beta-4B chain
WK: Tubulin alpha-3 chain
WL: Tubulin beta-4B chain
WM: Tubulin alpha-3 chain
WN: Tubulin beta-4B chain
WO: Tubulin alpha-3 chain
WP: Tubulin beta-4B chain
X: EF-hand domain-containing family member C2
X0: Spermatid-specific manchette-related protein 1
X1: Spermatid-specific manchette-related protein 1
X2: Spermatid-specific manchette-related protein 1
X3: Spermatid-specific manchette-related protein 1
X4: Protein phosphatase 1 regulatory subunit 32
X5: Protein phosphatase 1 regulatory subunit 32
X6: Testis-specific serine/threonine-protein kinase 6
X7: Testis-specific serine/threonine-protein kinase 6
X8: Testis-specific serine/threonine-protein kinase 6
XA: Cilia- and flagella-associated protein 20
XB: Cilia- and flagella-associated protein 20
XC: Cilia- and flagella-associated protein 20
XD: Cilia- and flagella-associated protein 20
XE: Cilia- and flagella-associated protein 20
XF: Cilia- and flagella-associated protein 20
XG: Cilia- and flagella-associated protein 20
XH: Cilia- and flagella-associated protein 20
Y: EF-hand domain-containing family member C2
Y0: Outer dense fiber protein 3
Y1: Outer dense fiber protein 3
Y2: Outer dense fiber protein 3
Y3: Outer dense fiber protein 3
Y4: Outer dense fiber protein 3
Y5: Outer dense fiber protein 3
YA: Parkin coregulated gene protein homolog
YB: Parkin coregulated gene protein homolog
YC: Parkin coregulated gene protein homolog
YD: Parkin coregulated gene protein homolog
YE: Parkin coregulated gene protein homolog
YF: Parkin coregulated gene protein homolog
YG: Parkin coregulated gene protein homolog
YH: Parkin coregulated gene protein homolog
Z: EF-hand domain-containing family member C2
Z1: Testis, prostate and placenta-expressed protein
Z2: Protein FAM166A
Z3: Protein FAM166A
Z4: Protein FAM166A
Z5: Protein FAM166A
a: Cilia- and flagella-associated protein 45
a1: Uncharacterized protein C10orf82 homolog
a2: Uncharacterized protein C10orf82 homolog
a3: Uncharacterized protein C10orf82 homolog
a4: Uncharacterized protein C10orf82 homolog
a6: Cilia- and flagella-associated protein 45
b: Cilia- and flagella-associated protein 45
b1: Coiled-coil domain-containing protein 105
b2: Coiled-coil domain-containing protein 105
b3: Coiled-coil domain-containing protein 105
b4: Coiled-coil domain-containing protein 105
b5: Coiled-coil domain-containing protein 105
c: Cilia- and flagella-associated protein 45
d: Cilia- and flagella-associated protein 45
e: Cilia- and flagella-associated protein 52
f: Cilia- and flagella-associated protein 52
g: Cilia- and flagella-associated protein 52
h: Enkurin
h1: Testis-expressed protein 43
h2: Testis-expressed protein 43
h3: Testis-expressed protein 43
h4: Testis-expressed protein 43
i: Enkurin
i1: Protein Flattop
i2: EF-hand calcium-binding domain-containing protein 6
i3: EF-hand calcium-binding domain-containing protein 6
i4: EF-hand calcium-binding domain-containing protein 6
j: Enkurin
j1: Nucleoside diphosphate kinase 7
k: Enkurin
l: Protein Flattop
m: Protein Flattop
n: Protein Flattop
o: Cilia- and flagella-associated protein 210
p: Cilia- and flagella-associated protein 210
q: Cilia- and flagella-associated protein 276
r: Cilia- and flagella-associated protein 276
s: Cilia- and flagella-associated protein 276
y: Piercer of microtubule wall 1 protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,904,7421108
Polymers27,729,286576
Non-polymers175,456532
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

-
Components

-
Cilia- and flagella-associated protein ... , 12 types, 33 molecules 07348AN1EFN2I1L1XAXBXCXDXEXFXGXHaa6bcdefgop...

#1: Protein Cilia- and flagella-associated protein 95


Mass: 26633.035 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CQC3
#3: Protein Cilia- and flagella-associated protein 53 / Coiled-coil domain-containing protein 11


Mass: 62036.609 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D439
#5: Protein Cilia- and flagella-associated protein 107


Mass: 23062.510 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q4KKZ1
#6: Protein Cilia- and flagella-associated protein 141


Mass: 12278.145 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9D9
#15: Protein Cilia- and flagella-associated protein 161


Mass: 34433.383 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6P8Y0
#18: Protein Cilia- and flagella-associated protein 68


Mass: 19552.668 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D131
#21: Protein Cilia- and flagella-associated protein 90 / Y regulated sperm protein


Mass: 20886.428 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAR0
#35: Protein
Cilia- and flagella-associated protein 20 / Gene trap locus 3 protein


Mass: 22781.389 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8BTU1
#39: Protein
Cilia- and flagella-associated protein 45 / Coiled-coil domain-containing protein 19 / Nasopharyngeal epithelium-specific protein 1


Mass: 65962.016 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9U9
#42: Protein Cilia- and flagella-associated protein 52 / WD repeat-containing protein 16


Mass: 68322.164 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q5F201
#47: Protein Cilia- and flagella-associated protein 210 / Coiled-coil domain-containing protein 173


Mass: 65266.520 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A0JLY1
#48: Protein Cilia- and flagella-associated protein 276


Mass: 18960.092 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAD0

-
EF-hand domain-containing family member ... , 2 types, 6 molecules 12WXYZ

#2: Protein EF-hand domain-containing family member B / Cilia- and flagella-associated protein 21


Mass: 95891.961 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8CDU5
#31: Protein
EF-hand domain-containing family member C2


Mass: 87758.023 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D485

+
Protein , 29 types, 521 molecules 56j1A0A1A2A3A4A5AAACAEAGAIAKAMAOBCBEBGBIBKBMBOCCCECGCICKCM...

#4: Protein Nucleoside diphosphate kinase 7 / NDK 7 / NDP kinase 7 / nm23-M7


Mass: 44489.828 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9QXL8, nucleoside-diphosphate kinase
#7: Protein
Tektin-1


Mass: 48713.035 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAJ2
#8: Protein ...
Tubulin alpha-3 chain / Alpha-tubulin 3/7 / Alpha-tubulin isotype M-alpha-3/7 / Tubulin alpha-3/alpha-7 chain


Mass: 50012.359 Da / Num. of mol.: 178 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: P05214, Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
#9: Protein ...
Tubulin beta-4B chain / Tubulin beta-2C chain


Mass: 49877.824 Da / Num. of mol.: 176 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: P68372
#10: Protein Meiosis-specific nuclear structural protein 1


Mass: 60353.012 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q61884
#11: Protein
Tektin-2 / Tektin-t / Testicular tektin


Mass: 50385.066 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q922G7
#12: Protein
Tektin-3


Mass: 56754.777 Da / Num. of mol.: 17 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6X6Z7
#13: Protein Sperm-associated antigen 8 / Sperm membrane protein 1 / SMP-1 / Sperm membrane protein BS-84


Mass: 50591.805 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q3V0Q6
#14: Protein
Tektin-4 / Testicular microtubules-related protein 4


Mass: 52121.449 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q149S1
#17: Protein
Protein FAM166A


Mass: 36659.566 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D4K5
#19: Protein
Cilia and flagella-associated protein 77


Mass: 32786.020 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A0A087WRI3
#20: Protein Family with sequence similarity 183, member B / Testis highly expressed protein 6


Mass: 16357.517 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: D2D553
#22: Protein
Protein FAM166C


Mass: 23097.957 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAS2
#24: Protein
Dual specificity phosphatase 21


Mass: 21527.574 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: Q9D9D8, protein-serine/threonine phosphatase, protein-tyrosine-phosphatase
#25: Protein
EF-hand domain-containing protein 1 / Myoclonin-1


Mass: 75235.422 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9T8
#26: Protein Testis expressed 49


Mass: 15528.622 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8CDT5
#28: Protein
Tektin bundle-interacting protein 1


Mass: 23987.262 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A6H6Q4
#30: Protein ...
Tektin-5


Mass: 62817.535 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: G5E8A8
#32: Protein
Spermatid-specific manchette-related protein 1 / Ciliated bronchial epithelial protein 1


Mass: 29977.191 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q2MH31
#33: Protein Protein phosphatase 1 regulatory subunit 32 / Protein IIIG9


Mass: 47873.078 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q148A4
#34: Protein Testis-specific serine/threonine-protein kinase 6 / TSK-6 / TSSK-6 / Testis-specific kinase 6 / Serine/threonine-protein kinase SSTK / Small ...TSK-6 / TSSK-6 / Testis-specific kinase 6 / Serine/threonine-protein kinase SSTK / Small serine/threonine kinase


Mass: 30332.943 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: Q925K9, non-specific serine/threonine protein kinase
#36: Protein
Outer dense fiber protein 3 / Outer dense fiber of sperm tails protein 3 / Sperm tail protein SHIPPO 1


Mass: 27690.973 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q920N1
#37: Protein
Parkin coregulated gene protein homolog / Hypertension-related protein 1-like protein / PARK2 coregulated gene protein


Mass: 27763.268 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAK2
#38: Protein Testis, prostate and placenta-expressed protein


Mass: 24985.697 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6IMH0
#40: Protein
Uncharacterized protein C10orf82 homolog


Mass: 25847.469 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CQT6
#41: Protein
Coiled-coil domain-containing protein 105


Mass: 57406.484 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D4K7
#43: Protein
Enkurin


Mass: 29587.521 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6SP97
#45: Protein
Protein Flattop / Cilia- and flagella-associated protein 126


Mass: 20575.123 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6P8X9
#46: Protein EF-hand calcium-binding domain-containing protein 6 / DJ-1-binding protein / DJBP


Mass: 176028.312 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6P1E8

-
Piercer of microtubule wall ... , 2 types, 2 molecules Gy

#16: Protein Piercer of microtubule wall 2 protein


Mass: 13728.513 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: V9GXK1
#49: Protein Piercer of microtubule wall 1 protein / Pierce1 / p53-induced expression in RB-null cells protein 1


Mass: 18862.852 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q5BN45

-
RIB43A-like with coiled-coils protein ... , 2 types, 5 molecules OPQRS

#23: Protein RIB43A-like with coiled-coils protein 1


Mass: 45032.160 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D0B8
#29: Protein RIB43A-like with coiled-coils protein 2


Mass: 45328.156 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D4Q1

-
Testis-expressed ... , 2 types, 9 molecules P3P4P5P6P7h1h2h3h4

#27: Protein
Testis-expressed sequence 37 protein


Mass: 21069.139 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAG4
#44: Protein
Testis-expressed protein 43


Mass: 16305.608 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D9I1

-
Non-polymers , 3 types, 532 molecules

#50: Chemical...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 178 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#51: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 178 / Source method: obtained synthetically / Formula: Mg
#52: Chemical...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 176 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM

-
Details

Has ligand of interestN

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: Mouse sperm DMT / Type: COMPLEX / Entity ID: #1-#49 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Mus musculus (house mouse)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 81000 X / Nominal defocus max: 2600 nm / Nominal defocus min: 1500 nm
Specimen holderSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 2.56 sec. / Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) / Num. of real images: 25135

-
Processing

SoftwareName: PHENIX / Version: 1.20.1_4487: phenix.real_space_refine / Classification: refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 95290 / Symmetry type: POINT

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