[English] 日本語

- EMDB-6445: Atomic structure of a non-enveloped virus reveals pH sensors for ... -
+
Open data
-
Basic information
Entry | Database: EMDB / ID: 6445 |
---|---|
Title | Atomic structure of a non-enveloped virus reveals pH sensors for a coordinated process of cell entry |
![]() | The whole map of BTV virion at pH 3.4 without imposing a reverse B-factor to boost high frequency signals |
![]() | BTV virion at pH 3.4 without imposing a reverse B-factor to boost high frequency signals: virus ![]() |
![]() | ![]() ![]() |
Source | ![]() ![]() |
Method | ![]() ![]() |
![]() | Zhang X / Patel A / Celma C / Roy P / Zhou ZH |
![]() | Journal: Nat. Struct. Mol. Biol. / Year: 2016 Title: Atomic model of a nonenveloped virus reveals pH sensors for a coordinated process of cell entry. ![]() |
Date | Deposition: Aug 27, 2015 / Header (metadata) release: Sep 23, 2015 / Map release: Dec 9, 2015 / Last update: Jan 20, 2016 |
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-
Download
![]() | ![]() |
---|---|
Header (meta data in XML format) | ![]() ![]() ![]() |
Images | ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6445 |
-
Links
-Related structure data
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.89 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire BTV virion at pH 3.4 without imposing a reverse B-factor to boost...
Entire | Name: BTV virion at pH 3.4 without imposing a reverse B-factor to boost high frequency signals Number of components: 1 |
---|
-Component #1: virus, Bluetongue virus 1
Virus | Name: Bluetongue virus 1 / Class: VIRION / Empty: No / Enveloped: No / Isolate: SPECIES |
---|---|
Species | Species: ![]() ![]() |
Source (natural) | Host Species: ![]() ![]() ![]() |
-Experimental details
-
Sample preparation
Specimen | Specimen state: particle / Method: ![]() |
---|---|
Sample solution | Buffer solution: citrate buffer / pH: 3.4 |
Support film | thin carbon on a lacey holey carbon support film |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 90 K / Humidity: 100 % |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
![]() | Microscope: FEI TITAN KRIOS / Date: May 11, 2011 |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: FEI TITAN KRIOS AUTOGRID HOLDER |
Camera | Detector: GATAN ULTRASCAN 4000 (4k x 4k) |
-Image acquisition
Image acquisition | Number of digital images: 350 / Sampling size: 15 microns / Bit depth: 32 |
---|
-
Image processing
![]() | Method: ![]() |
---|---|
3D reconstruction | Algorithm: reference match / Software: Frealign / CTF correction: each particle / Resolution: 3.3 Å / Resolution method: FSC 0.143, semi-independent |