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- EMDB-63296: Cryo-EM structure of CTR-bound type III-B CRISPR-Cas effector complex -

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Basic information

Entry
Database: EMDB / ID: EMD-63296
TitleCryo-EM structure of CTR-bound type III-B CRISPR-Cas effector complex
Map data
Sample
  • Complex: CTR-bound type III-B CRISPR-Cas effector complex
    • Protein or peptide: x 6 types
    • RNA: x 2 types
  • Ligand: x 3 types
KeywordsCRISPR-Cas complex / adaptive immunity / RNA degradation / second messenger / IMMUNE SYSTEM/RNA / IMMUNE SYSTEM-RNA complex
Function / homology
Function and homology information


defense response to virus / cytoplasm
Similarity search - Function
CRISPR-associated protein (Cas_Cmr5) / CRISPR-associated protein, TM1791 / CRISPR-associated protein, Cmr3 / CRISPR-associated protein (Cas_Cmr3) / CRISPR-associated protein, Cmr5 / CRISPR-associated RAMP Cmr4 / AF1862-like domain superfamily / CRISPR-associated protein Cmr2 / CRISPR-associated protein Cmr2, N-terminal / CRISPR-Cas system, Cmr2 subunit, D1 domain, cysteine cluster ...CRISPR-associated protein (Cas_Cmr5) / CRISPR-associated protein, TM1791 / CRISPR-associated protein, Cmr3 / CRISPR-associated protein (Cas_Cmr3) / CRISPR-associated protein, Cmr5 / CRISPR-associated RAMP Cmr4 / AF1862-like domain superfamily / CRISPR-associated protein Cmr2 / CRISPR-associated protein Cmr2, N-terminal / CRISPR-Cas system, Cmr2 subunit, D1 domain, cysteine cluster / CRISPR-associated protein Cmr2, N-terminal / : / Cas10/Cmr2, second palm domain / CRISPR type III-associated protein / RAMP superfamily / Reverse transcriptase/Diguanylate cyclase domain
Similarity search - Domain/homology
CRISPR type III-B/RAMP module-associated protein Cmr5 / RAMP superfamily protein / Type III-B CRISPR-associated protein Cas10/Cmr2 / Uncharacterized protein / CRISPR-associated protein Cmr3 / Type III-B CRISPR module RAMP protein Cmr4
Similarity search - Component
Biological speciesBacteroides fragilis (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.85 Å
AuthorsJin X / Duan B / Chen Z / Zhao B
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Mechanisms of SAM-AMP synthesis and degradation in antiviral type III CRISPR signaling.
Authors: Duan B / Jin X / Chen Z / Zhao B
History
DepositionJan 28, 2025-
Header (metadata) releaseNov 26, 2025-
Map releaseNov 26, 2025-
UpdateNov 26, 2025-
Current statusNov 26, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_63296.png

Downloads & links

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Map

FileDownload / File: emd_63296.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 360 pix.
= 335.52 Å		0.93 Å/pix.
x 360 pix.
= 335.52 Å		0.93 Å/pix.
x 360 pix.
= 335.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.932 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.14387429 - 38.241126999999999
Average (Standard dev.)-0.0055629304 (±0.5715779)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 335.52 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_63296_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_63296_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : CTR-bound type III-B CRISPR-Cas effector complex

EntireName: CTR-bound type III-B CRISPR-Cas effector complex
Components
  • Complex: CTR-bound type III-B CRISPR-Cas effector complex
    • Protein or peptide: Cmr1
    • Protein or peptide: CRISPR-associated protein Cmr3
    • Protein or peptide: Type III-B CRISPR module RAMP protein Cmr4
    • Protein or peptide: CRISPR type III-B/RAMP module-associated protein Cmr5
    • Protein or peptide: RAMP superfamily protein
    • RNA: CTR
    • RNA: RNA (49-MER)
    • Protein or peptide: Type III-B CRISPR-associated protein Cas10/Cmr2
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: S-ADENOSYLMETHIONINE
  • Ligand: MANGANESE (II) ION

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Supramolecule #1: CTR-bound type III-B CRISPR-Cas effector complex

SupramoleculeName: CTR-bound type III-B CRISPR-Cas effector complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#8
Source (natural)Organism: Bacteroides fragilis (bacteria)

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Macromolecule #1: Cmr1

MacromoleculeName: Cmr1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 55.34025 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MNQLTAILKQ HTPMIHFQHN ESGATLRASE VKPLLDKFIL TKLGNGDIRE GRLYAKKNNW LIDNEKNYAL NYKLSISLQK KSRLEYLIT SSTFPLPTER PSNFFTIQNS PYFAQEKCVG INTNSTIILK KSNSDPRKKE AEFKEKNWSQ IDKKGLEWQD F TIKIFSLK ...String:
MNQLTAILKQ HTPMIHFQHN ESGATLRASE VKPLLDKFIL TKLGNGDIRE GRLYAKKNNW LIDNEKNYAL NYKLSISLQK KSRLEYLIT SSTFPLPTER PSNFFTIQNS PYFAQEKCVG INTNSTIILK KSNSDPRKKE AEFKEKNWSQ IDKKGLEWQD F TIKIFSLK GDLINKIQTY LPAFFICHNF GTRNNKGFGS FTVEYINNQK NICNVEDTLK ENFAFVYKKK IALSRQSTLD FI YIYNQIF STIKKDYQIL KSGYNFRNEY IKSLLFCYFV SKYPNYRWEK RKMKQLIKAR GYELKGDHSP ISGIRENDNS WND PNPNGY NYAYIRAILG LAEQYEFQLE TPYQKAIVKI KSANNCISRY KSPLLFKIIN NSIYLVGNEI NTEILNKPFQ YSYI EQTKN KNMRTGKSEI TERTMHINEI EMNYKNRINY HYTPTSFSLI DFMQYAMSYK KNGKNILNYI PLKQ

UniProtKB: Uncharacterized protein

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Macromolecule #2: CRISPR-associated protein Cmr3

MacromoleculeName: CRISPR-associated protein Cmr3 / type: protein_or_peptide / ID: 2 / Details: deletions / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 49.059207 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MSHHHHHHHH DGKPIPNPLL GLDSTGSDQT ENSGENLYFQ GANAMNRHYL ITLTPMDWFF FGGERTLDDG KSADYISHSN KFPQQSALL GMIRYQLLKQ HNLLSQFPYT ENKPTEKEIM KTLIGEQSFR MTERKAKSLG LGVIKQISPL MLIECKDDTS S RSIYFPLP ...String:
MSHHHHHHHH DGKPIPNPLL GLDSTGSDQT ENSGENLYFQ GANAMNRHYL ITLTPMDWFF FGGERTLDDG KSADYISHSN KFPQQSALL GMIRYQLLKQ HNLLSQFPYT ENKPTEKEIM KTLIGEQSFR MTERKAKSLG LGVIKQISPL MLIECKDDTS S RSIYFPLP LDDGYKVSFN ETSNEDKVFY NGIECPIPNV YPASEEQDSG NQKRKFFDHK TYNNYLFWCT QGNNQIKKLL SD EIWISKM QIGITKHVEE GEDNDKSFYK QEFLQLKKSF IYAFYITLSG ESELSSDIIQ LGGQRSVFRM EVESIEENSD IQE KYQTAA QFLTQSDRLL ILSPTYVDNL KELSALCNFM WSDSIVFRNI QTTNASNFYG KPIKSSSKYH FLKPGSVLYF KQGK RKEVE KLLMDYTYLR LSGYNIYI

UniProtKB: CRISPR-associated protein Cmr3

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Macromolecule #3: Type III-B CRISPR module RAMP protein Cmr4

MacromoleculeName: Type III-B CRISPR module RAMP protein Cmr4 / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 31.537041 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MTTRMYVINT LSNMHVGSGE VNYGVIANLI QRDSVTNLPN INSSGLKGAI REYFKENEDL VRELFGSAPR DEKTLPGKVR FFEANLLSM PVRSDKVPFL MAISDEVLQE LITKMKFFNC EEATQYISHL STLLDNIKTQ AQGTDFAYVF DPLLQGAIIE E VSIRATCP ...String:
MTTRMYVINT LSNMHVGSGE VNYGVIANLI QRDSVTNLPN INSSGLKGAI REYFKENEDL VRELFGSAPR DEKTLPGKVR FFEANLLSM PVRSDKVPFL MAISDEVLQE LITKMKFFNC EEATQYISHL STLLDNIKTQ AQGTDFAYVF DPLLQGAIIE E VSIRATCP SHIPLQPSLK KLLGDRLVIL SHKYFSILSD DNHLPVLSRN NLENGQSANL WYEQVLPRYS RLYFMLMDGN AQ SEYLKKF RDTLCTPSTI IQIGANASIG YGYCQISELS PF

UniProtKB: Type III-B CRISPR module RAMP protein Cmr4

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Macromolecule #4: CRISPR type III-B/RAMP module-associated protein Cmr5

MacromoleculeName: CRISPR type III-B/RAMP module-associated protein Cmr5 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 15.273493 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MKISKKQIEY AIEALRANNI ITNDNQYPKV FKGYISSFGA AVIQSGLIPA IIFFENEDND ANADRHKIIG VLKDIINAMR QQYTVTDAT ILVSSQIPAN YSMAQYIIEH GNTDQLLKEI TEAAVAMKLA LRMYKSE

UniProtKB: CRISPR type III-B/RAMP module-associated protein Cmr5

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Macromolecule #5: RAMP superfamily protein

MacromoleculeName: RAMP superfamily protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 35.951883 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MPKNYTLQNA SNLGWLFYKD YYRQEPNVDF ISTQGKESDT TADFFRKTNQ RITAYQLNSE SPLVAAFNNH FGTPLQLKTI YPGLITGSG LPHQTGSKGE FKLGFQFDYT TGLPYIPGSS IKGTLRSMFP FSLKDKGSTK RILPEYRKER MEYIRDLIIE V TNINEISD ...String:
MPKNYTLQNA SNLGWLFYKD YYRQEPNVDF ISTQGKESDT TADFFRKTNQ RITAYQLNSE SPLVAAFNNH FGTPLQLKTI YPGLITGSG LPHQTGSKGE FKLGFQFDYT TGLPYIPGSS IKGTLRSMFP FSLKDKGSTK RILPEYRKER MEYIRDLIIE V TNINEISD TEIQALEYAI FTNSTPSGKT IEFSLEEKDV FYDAFVADSK DGVMLSDDYI TPHGENPLKD PKPILFLKIR PD VTINFYF KLCTTHLYKE KVCSSKQIEE IKKQNDFSSS DYKMITAHQK RNLFEKILLC IGIGAKTNIG YGQLKKL

UniProtKB: RAMP superfamily protein

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Macromolecule #8: Type III-B CRISPR-associated protein Cas10/Cmr2

MacromoleculeName: Type III-B CRISPR-associated protein Cas10/Cmr2 / type: protein_or_peptide / ID: 8 / Details: deletions / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 69.247078 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKYIAITLGP ITRTIEMAES TKELWAASYF FSYLAKKIVE PFVKKNRTFQ LPLINEEMQK PHCGAGLFPD RYIFKSEPGD LELLKQHSD QVLIEIAGHI ASPSLPGTAK DVSQIYHYLK SYIKIYFIER TLESDDPHVV IPACEKYLNI IENQETFPEQ E ETMISHQK ...String:
MKYIAITLGP ITRTIEMAES TKELWAASYF FSYLAKKIVE PFVKKNRTFQ LPLINEEMQK PHCGAGLFPD RYIFKSEPGD LELLKQHSD QVLIEIAGHI ASPSLPGTAK DVSQIYHYLK SYIKIYFIER TLESDDPHVV IPACEKYLNI IENQETFPEQ E ETMISHQK SDFLKFLITN VNGKIYRKDK NSIPRFTGSF LTRDAFGDMN GERLFESILE ISASELNINI QQKALEVITA NE KNKGEKY SDQIWDAEEI ILNDNKAQLR PYHKYIAIIK SDGDSMGETI KSMGAYNIPI TQLSKALLSF NIESINEIVA YGG KPIFIG GDDLLCFAPV CCNGNNVFNL VEKLSTCFDQ CINQHLQQYI NACSEAQRPL PSLSFGISIT YHKYPMFEAL HTTD YLLEM VAKDNLFKYT LSNKNILNEN MKRFILKNKL AFSLQKHSGQ IYHTAMSKKG KSYVKFNMLL QKYILKNKDM SKTQE SEKF LSSVIQMIRA HAEILQIILQ NEDKRTEMLK NYFDNNFNES CHLGYTGLFE DIQTLLCLRY QENIQDYQNR NEIIQQ NTI LTSDEKEILI VSPAMDAIHT IFTALQFIHF INYNKDE

UniProtKB: Type III-B CRISPR-associated protein Cas10/Cmr2

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Macromolecule #6: CTR

MacromoleculeName: CTR / type: rna / ID: 6 / Details: deletions / Number of copies: 1
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 15.756532 KDa
SequenceString:
CACAAGGGAC GCCACUUCAU GGAAAUAAAU CACUCACGAA UAGACACGA

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Macromolecule #7: RNA (49-MER)

MacromoleculeName: RNA (49-MER) / type: rna / ID: 7 / Details: deletions / Number of copies: 1
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 15.671214 KDa
SequenceString:
AUUAAGACAU UCGUGAGUGA UUUAUUUCCA UGAAGUGGCG UCCCUUGUG

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Macromolecule #9: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 9 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #10: S-ADENOSYLMETHIONINE

MacromoleculeName: S-ADENOSYLMETHIONINE / type: ligand / ID: 10 / Number of copies: 1 / Formula: SAM
Molecular weightTheoretical: 398.437 Da
Chemical component information

ChemComp-SAM:
S-ADENOSYLMETHIONINE

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Macromolecule #11: MANGANESE (II) ION

MacromoleculeName: MANGANESE (II) ION / type: ligand / ID: 11 / Number of copies: 1 / Formula: MN
Molecular weightTheoretical: 54.938 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.85 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 82768
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

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