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- EMDB-61959: EF-G2 bound 70S ribosome complex of M. smegmatis -

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Basic information

Entry
Database: EMDB / ID: EMD-61959
TitleEF-G2 bound 70S ribosome complex of M. smegmatis
Map data
Sample
  • Complex: EF-G2 bound 70S ribosome complex of M. smegmatis
    • RNA: x 5 types
    • Protein or peptide: x 53 types
  • Ligand: x 4 types
KeywordsMycobacterium 70S ribosome / stationary phase / elongation factor G2 / non-canonical factor / RIBOSOME
Function / homology
Function and homology information


ribosome disassembly / translation elongation factor activity / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit ...ribosome disassembly / translation elongation factor activity / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / GTP binding / RNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
: / Elongation factor G, domain III / EFG, domain V / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like ...: / Elongation factor G, domain III / EFG, domain V / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / Elongation Factor G, domain II / Elongation Factor G, domain III / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L10 / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / EF-G domain III/V-like / : / : / Ribosomal protein S14, type Z / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein L11, bacterial-type / Elongation factor Tu domain 2 / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L10-like domain superfamily / : / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L18, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S11, bacterial-type
Similarity search - Domain/homology
Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein bL12 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 ...Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein bL12 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Uncharacterized protein / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL32 / Elongation factor G-like protein / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein bS18B / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL25
Similarity search - Component
Biological speciesMycolicibacterium smegmatis MC2 155 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.7 Å
AuthorsSengupta J / Baid P
Funding support India, 3 items
OrganizationGrant numberCountry
Science and Engineering Research Board (SERB)SPF/2021/000141 India
Science and Engineering Research Board (SERB)CRG/2019/001788 India
Council of Scientific & Industrial Research (CSIR)MLP-139 India
CitationJournal: FEBS J / Year: 2025
Title: Cryo-EM structural analyses reveal a unique role for elongation factor G2 (EF-G2) in Mycobacteria.
Authors: Priya Baid / Jayati Sengupta /
Abstract: The gene-encoding translation elongation factor G (EF-G) has undergone gene duplication across various bacterial species including Mycobacteria, and in mammalian mitochondria, leading to the ...The gene-encoding translation elongation factor G (EF-G) has undergone gene duplication across various bacterial species including Mycobacteria, and in mammalian mitochondria, leading to the emergence of the paralogue elongation factor G2 (EF-G2). Our study reveals that mycobacterial EF-G2, unlike EF-G1, neither participates in ribosome-recycling nor significantly contributes to overall translation, suggesting that it plays an alternative role in Mycobacteria. Remarkably, our investigation found a significant overexpression of mycobacterial EF-G2 during the stationary growth phase. Moreover, EF-G2 lacks ribosome-dependent GTPase activity, an observation consistent with previous reports. Cryo-EM analysis of the M. smegmatis 70S ribosome purified from the nutrient-starved (stationary) phase and complexed with EF-G2 unveiled the structural basis for its inability to hydrolyse GTP in a ribosome-dependent manner. Furthermore, we report an unprecedented binding mode of two EF-G2 copies on the 50S ribosomal subunit that impedes subunit association, thereby preventing the formation of active 70S ribosomes. Thus, instead of performing canonical functions, mycobacterial EF-G2 acts as a translation repressor during nutrient starvation. Altogether, our findings shed light on the multifaceted mechanisms by which EF-G2 modulates protein synthesis under nutrient-limited conditions, providing insights into adaptive strategies employed by Mycobacteria to survive in hostile environments.
History
DepositionOct 16, 2024-
Header (metadata) releaseJul 9, 2025-
Map releaseJul 9, 2025-
UpdateJul 9, 2025-
Current statusJul 9, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_61959.png

Downloads & links

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Map

FileDownload / File: emd_61959.map.gz / Format: CCP4 / Size: 163.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
1.38 Å/pix.
x 350 pix.
= 483. Å		1.38 Å/pix.
x 350 pix.
= 483. Å		1.38 Å/pix.
x 350 pix.
= 483. Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.38 Å
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Contour LevelBy AUTHOR: 0.00715
Minimum - Maximum-0.02739546 - 0.09018755
Average (Standard dev.)0.00024104731 (±0.006317157)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions350350350
Spacing350350350
CellA=B=C: 483.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_61959_half_map_1.map
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Half map: #1

Fileemd_61959_half_map_2.map
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Sample components

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Entire : EF-G2 bound 70S ribosome complex of M. smegmatis

EntireName: EF-G2 bound 70S ribosome complex of M. smegmatis
Components
  • Complex: EF-G2 bound 70S ribosome complex of M. smegmatis
    • RNA: P/P-site Phe-tRNA(Phe)
    • Protein or peptide: 50S ribosomal protein bL37
    • RNA: mRNA fragment
    • Protein or peptide: Large ribosomal subunit protein bL12
    • Protein or peptide: Translation elongation factor EF-G
    • RNA: 16S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: Small ribosomal subunit protein uS4
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Small ribosomal subunit protein bS6
    • Protein or peptide: Small ribosomal subunit protein uS7
    • Protein or peptide: Small ribosomal subunit protein uS8
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: Small ribosomal subunit protein uS10
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: Small ribosomal subunit protein uS14B
    • Protein or peptide: Small ribosomal subunit protein uS15
    • Protein or peptide: Small ribosomal subunit protein bS16
    • Protein or peptide: Small ribosomal subunit protein uS17
    • Protein or peptide: Small ribosomal subunit protein bS18B
    • Protein or peptide: Small ribosomal subunit protein uS19
    • Protein or peptide: Small ribosomal subunit protein bS20
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Small ribosomal subunit protein uS2
    • Protein or peptide: Large ribosomal subunit protein bL25
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • Protein or peptide: Large ribosomal subunit protein bL32
    • Protein or peptide: Large ribosomal subunit protein bL33A
    • Protein or peptide: Large ribosomal subunit protein bL34
    • Protein or peptide: Large ribosomal subunit protein bL35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: Large ribosomal subunit protein bL31
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein uL5
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: Large ribosomal subunit protein uL10
    • Protein or peptide: Large ribosomal subunit protein uL11
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: Large ribosomal subunit protein uL15
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein bL17
    • Protein or peptide: Large ribosomal subunit protein uL18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: Large ribosomal subunit protein bL21
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Large ribosomal subunit protein uL24
    • RNA: 23S ribosomal RNA
  • Ligand: PHENYLALANINE
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: EF-G2 bound 70S ribosome complex of M. smegmatis

SupramoleculeName: EF-G2 bound 70S ribosome complex of M. smegmatis / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#58
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 2.7 MDa

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Macromolecule #1: P/P-site Phe-tRNA(Phe)

MacromoleculeName: P/P-site Phe-tRNA(Phe) / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 24.420508 KDa
SequenceString:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG ACUCAUAACC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

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Macromolecule #3: mRNA fragment

MacromoleculeName: mRNA fragment / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 5.866619 KDa
SequenceString:
AGGAGGUAAA AAUGCCAA

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Macromolecule #6: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 6 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 490.111719 KDa
SequenceString: UUUGGAGAGU UUGAUCCUGG CUCAGGACGA ACGCUGGCGG CGUGCUUAAC ACAUGCAAGU CGAACGGAAA GGCCCUUUCG GGGGUACUC GAGUGGCGAA CGGGUGAGUA ACACGUGGGU GAUCUGCCCU GCACUUUGGG AUAAGCCUGG GAAACUGGGU C UAAUACCG ...String:
UUUGGAGAGU UUGAUCCUGG CUCAGGACGA ACGCUGGCGG CGUGCUUAAC ACAUGCAAGU CGAACGGAAA GGCCCUUUCG GGGGUACUC GAGUGGCGAA CGGGUGAGUA ACACGUGGGU GAUCUGCCCU GCACUUUGGG AUAAGCCUGG GAAACUGGGU C UAAUACCG AAUACACCCU GCUGGUCGCA UGGCCUGGUA GGGGAAAGCU UUUGCGGUGU GGGAUGGGCC CGCGGCCUAU CA GCUUGUU GGUGGGGUGA UGGCCUACCA AGGCGACGAC GGGUAGCCGG CCUGAGAGGG UGACCGGCCA CACUGGGACU GAG AUACGG CCCAGACUCC UACGGGAGGC AGCAGUGGGG AAUAUUGCAC AAUGGGCGCA AGCCUGAUGC AGCGACGCCG CGUG AGGGA UGACGGCCUU CGGGUUGUAA ACCUCUUUCA GCACAGACGA AGCGCAAGUG ACGGUAUGUG CAGAAGAAGG ACCGG CCAA CUACGUGCCA GCAGCCGCGG UAAUACGUAG GGUCCGAGCG UUGUCCGGAA UUACUGGGCG UAAAGAGCUC GUAGGU GGU UUGUCGCGUU GUUCGUGAAA ACUCACAGCU UAACUGUGGG CGUGCGGGCG AUACGGGCAG ACUAGAGUAC UGCAGGG GA GACUGGAAUU CCUGGUGUAG CGGUGGAAUG CGCAGAUAUC AGGAGGAACA CCGGUGGCGA AGGCGGGUCU CUGGGCAG U AACUGACGCU GAGGAGCGAA AGCGUGGGGA GCGAACAGGA UUAGAUACCC UGGUAGUCCA CGCCGUAAAC GGUGGGUAC UAGGUGUGGG UUUCCUUCCU UGGGAUCCGU GCCGUAGCUA ACGCAUUAAG UACCCCGCCU GGGGAGUACG GCCGCAAGGC UAAAACUCA AAGGAAUUGA CGGGGGCCCG CACAAGCGGC GGAGCAUGUG GAUUAAUUCG AUGCAACGCG AAGAACCUUA C CUGGGUUU GACAUGCACA GGACGCCGGC AGAGAUGUCG GUUCCCUUGU GGCCUGUGUG CAGGUGGUGC AUGGCUGUCG UC AGCUCGU GUCGUGAGAU GUUGGGUUAA GUCCCGCAAC GAGCGCAACC CUUGUCUCAU GUUGCCAGCA CGUUAUGGUG GGG ACUCGU GAGAGACUGC CGGGGUCAAC UCGGAGGAAG GUGGGGAUGA CGUCAAGUCA UCAUGCCCCU UAUGUCCAGG GCUU CACAC AUGCUACAAU GGCCGGUACA AAGGGCUGCG AUGCCGUGAG GUGGAGCGAA UCCUUUCAAA GCCGGUCUCA GUUCG GAUC GGGGUCUGCA ACUCGACCCC GUGAAGUCGG AGUCGCUAGU AAUCGCAGAU CAGCAACGCU GCGGUGAAUA CGUUCC CGG GCCUUGUACA CACCGCCCGU CACGUCAUGA AAGUCGGUAA CACCCGAAGC CGGUGGCCUA ACCCUUGUGG AGGGAGC CG UCGAAGGUGG GAUCGGCGAU UGGGACGAAG UCGUAACAAG GUAGCCGUAC CGGAAGGUGC GGCUGGAUC

GENBANK: GENBANK: CP000480.1

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Macromolecule #7: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 7 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 38.061816 KDa
SequenceString:
GUUACGGCGG UCCAUAGCGG CAGGGAAACG CCCGGUCCCA UCCCGAACCC GGAAGCUAAG CCUGCCAGCG CCGAUGAUAC UACCCAUCC GGGUGGAAAA GUAGGACACC GCCGAACAC

GENBANK: GENBANK: CP000480.1

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Macromolecule #58: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 58 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 1.014391625 MDa
SequenceString: UUGUAAGUGU UUAAGGGCGC AUGGUGGAUG CCUUGGCACU GGGAGCCGAU GAAGGACGUA GGAGGCUGCG AUAAGCCUCG GGGAGCUGU CAACCGAGCG UUGAUCCGAG GAUGUCCGAA UGGGGAAACC CGGCACGAGU GAUGUCGUGU CACCAGGCGC U GAAUAUAU ...String:
UUGUAAGUGU UUAAGGGCGC AUGGUGGAUG CCUUGGCACU GGGAGCCGAU GAAGGACGUA GGAGGCUGCG AUAAGCCUCG GGGAGCUGU CAACCGAGCG UUGAUCCGAG GAUGUCCGAA UGGGGAAACC CGGCACGAGU GAUGUCGUGU CACCAGGCGC U GAAUAUAU AGGCGUCUGG GGGGAACGCG GGGAAGUGAA ACAUCUCAGU ACCCGUAGGA AGAGAAAACA AAAUGUGAUU CC GUGAGUA GUGGCGAGCG AAAGCGGAGG AUGGCUAAAC CGUAUGCAUG UGAUACCGGG UAGGGGUUGU GUGUGCGGGG UUG UGGGAC CUAUCUUUCC GGCUCUACCU GGCUGGAGGG CAGUGAGAAA AUGUUGUGGU UAGCGGAAAU GGCUUGGGAU GGCC UGCCG UAGACGGUGA GAGCCCGGUA CGUGAAAACC CGACGUCUGU CUUGAUGGUG UUCCCGAGUA GCAGCGGGCC CGUGG AAUC UGCUGUGAAU CUGCCGGGAC CACCCGGUAA GCCUGAAUAC UUCCCAGUGA CCGAUAGCGG AUUAGUACCG UGAGGG AAU GGUGAAAAGU ACCCCGGGAG GGGAGUGAAA GAGUACCUGA AACCGUGCGC UUACAAUCCG UCAGAGCCCU CGACGUG UC GUGGGGUGAU GGCGUGCCUU UUGAAGAAUG AGCCUGCGAG UCAGGGACAU GUCGCGAGGU UAACCCGGGU GGGGUAGC C GCAGCGAAAG CGAGUCUGAA UAGGGCGUAU CCACACAAGA GUGUGUGGUG UAGUGGUGUG UUCUGGACCC GAAGCGGAG UGAUCUACCC AUGGCCAGGG UGAAGCGCGG GUAAGACCGC GUGGAGGCCC GAACCCACUU AGGUUGAAGA CUGAGGGGAU GAGCUGUGG GUAGGGGUGA AAGGCCAAUC AAACUCCGUG AUAGCUGGUU CUCCCCGAAA UGCAUUUAGG UGCAGCGUCG C AUGUUUCU UGCCGGAGGU AGAGCUACUG GAUGGCCGAU GGGCCCCACA GGGUUACUGA CGUCAGCCAA ACUCCGAAUG CC GGUAAGU CCAAGAGUGC GGCAGUGAGA CGGCGGGGGA UAAGCUCCGU GCGUCGAGAG GGAAACAGCC CAGAUCGCCG GCU AAGGCC CCUAAGCGUG UGCUAAGUGG AAAAGGAUGU GCAGUCGCGA AGACAACCAG GAGGUUGGCU UAGAAGCAGC CACC CUUGA AAGAGUGCGU AAUAGCUCAC UGGUCAAGUG AUUGUGCGCC GAUAAUGUAG CGGGGCUCAA GCACACCGCC GAAGC CGCG GCAGCCAACG UGUUGGCUGG GUAGGGGAGC GUCCUGCAUC CGGUGAAGCC GCCGAGUGAU CGAGUGGUGG AGGGUG UGG GAGUGAGAAU GCAGGCAUGA GUAGCGAUUA GGCAAGUGAG AACCUUGCCC GCCGAAAGAC CAAGGGUUCC UGGGCCA GG CCAGUCCGCC CAGGGUGAGU CGGGACCUAA GGCGAGGCCG ACAGGCGUAG UCGAUGGACA ACGGGUUGAU AUUCCCGU A CCCGUGUAUG UGCGUCCAUG AUGAAUCAGC GGUACUAACC AUCCAAAACC ACCGUGACCG CACCUUUCGG GGUGUGGCG UUGGUGGGGC UGCAUGGGAC CUUCGUUGGU AGUAGUCAAG CGAUGGGGUG ACGCAGGAAG GUAGCCGUAC CGGUCAGUGG UAAUACCGG GGUAAGCCUG UAGGGAGUCA GAUAGGUAAA UCCGUCUGGC AUAUAUCCUG AGAGGUGAUG CAUAGCCGAG U GAGGCGAA UUCGGUGAUC CUAUGCUGCC GAGAAAAGCC UCUAGCGAGG ACAUACACGG CCCGUACCCC AAACCAACAC AG GUGGUCA GGUAGAGAAU ACUAAGGCGU ACGAGUGAAC UAUGGUUAAG GAACUCGGCA AAAUGCCCCC GUAACUUCGG GAG AAGGGG GACCCACAUG GCGUGUAAGC CUUUACGGCC CAAGCGUGAG UGGGUGGCAC AAACCAGUGA GAAGCGACUG UUUA CUAAA AACACAGGUC CGUGCGAAGU CGCAAGACGA UGUAUACGGA CUGACGCCUG CCCGGUGCUG GAAGGUUAAG AGGAC CCGU UAACUCCCUU UGGGGGUGAA GCGGAGAAUU UAAGCCCCAG UAAACGGCGG UGGUAACUAU AACCAUCCUA AGGUAG CGA AAUUCCUUGU CGGGUAAGUU CCGACCUGCA CGAAUGGCGU AACGACUUCU CAACUGUCUC AACCAUAGAC UCGGCGA AA UUGCACUACG AGUAAAGAUG CUCGUUACGC GCGGCAGGAC GAAAAGACCC CGGGACCUUC ACUACAACUU GGUAUUGG U GCUCGAUACG GUUUGUGUAG GAUAGGUGGG AGACUGUGAA GCUCACACGC CAGUGUGGGU GGAGUCGUUG UUGAAAUAC CACUCUGAUC GUAUUGGGCC UCUAACCUCG GACCGUAUAU CCGGUUCAGG GACAGUGCCU GGUGGGUAGU UUAACUGGGG CGGUUGCCU CCUAAAAUGU AACGGAGGCG CCCAAAGGUU CCCUCAACCU GGACGGCAAU CAGGUGUUGA GUGUAAGUGC A CAAGGGAG CUUGACUGCG AGACGGACAU GUCGAGCAGG GACGAAAGUC GGGACUAGUG AUCCGGCACC UCUGAGUGGA AG GGGUGUC GCUCAACGGA UAAAAGGUAC CCCGGGGAUA ACAGGCUGAU CUUCCCCAAG AGUCCAUAUC GACGGGAUGG UUU GGCACC UCGAUGUCGG CUCGUCGCAU CCUGGGGCUG GAGCAGGUCC CAAGGGUUGG GCUGUUCGCC CAUUAAAGCG GCAC GCGAG CUGGGUUUAG AACGUCGUGA GACAGUUCGG UCUCUAUCCG CCGCGCGCGU CAGAAGCUUG AGGAAACCUG UCCCU AGUA CGAGAGGACC GGGACGGACG AACCUCUGGU AUACCAGUUG UCCCACCAGG GGCACGGCUG GAUAGCCACG UUCGGA CAG GAUAACCGCU GAAAGCAUCU AAGCGGGAAA CCUCUUCCAA GACCAGGCUU CUCACCCUCU AGGAGGGAUA AGGCCCC CC GCAGACCACG GGAUUGAUAG ACCAGACCUG GAAGCCUAGU AAUAGGUGCA GGGAACUGGC ACUAACCGGC CGAAAACU U ACAACA

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Macromolecule #2: 50S ribosomal protein bL37

MacromoleculeName: 50S ribosomal protein bL37 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 2.710179 KDa
SequenceString:
AKRGRKKRDR KHSKANHGKR PNA

UniProtKB: Uncharacterized protein

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Macromolecule #4: Large ribosomal subunit protein bL12

MacromoleculeName: Large ribosomal subunit protein bL12 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 7.199378 KDa
SequenceString:
EFDVILEGAG EKKIGVIKVV REIVSGLGLK EAKDLVDSAP KPLLEKVTKE AADDAKAKLE AAGASVTVK

UniProtKB: Large ribosomal subunit protein bL12

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Macromolecule #5: Translation elongation factor EF-G

MacromoleculeName: Translation elongation factor EF-G / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 75.420797 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: GSVPTAERPA AIRNVALVGP SGGGKTTLVE ALLVAGGVLT RPGSVADGST VCDFDEAEIA QQRSVSLALA SLSHNGIKVN LIDTPGYAD FVGELRAGLR AADCALFVIA ANETIDEPTK TLWQECNEVQ MPRAVVITKL DHPRANYDAA LTAAQEAFGD K VAPLYFPV ...String:
GSVPTAERPA AIRNVALVGP SGGGKTTLVE ALLVAGGVLT RPGSVADGST VCDFDEAEIA QQRSVSLALA SLSHNGIKVN LIDTPGYAD FVGELRAGLR AADCALFVIA ANETIDEPTK TLWQECNEVQ MPRAVVITKL DHPRANYDAA LTAAQEAFGD K VAPLYFPV GDGESCKGVV GLLTRTYYDY SGGTHTARPP DGSHDAAIAE LRGSLIEGVI EESEDETLME RYLGGEEIDE AV LIADLEK AVARASFFPV IPVCSQSGVG TLELLDIISR GFPAPPEHQL PEVFTPQGAP RKPLSCDPDG PLLAEVVKTT SDP YVGRVS LVRVFSGTIR PDATVHVSGH FSAFTDTSGS HGAAAAADGS THSHADHDED ERIGTLSFPL GKQQRPAPTV VAGD ICAIG RLSRAETGDT LSDKTDPLVL RPWTMPDPLL PIAVAPRAKT DEDKLSVGLQ RLAAEDPTLR IEQNPETHQI VLWCM GEAH AAVVLDALSR RYGVSVDTVE LRVPLRETFA GKATGHGRHV KQSGGHGQYA VCDIEVEPLP EGSGFEFVDK VVGGAV PRQ FIPSVEKGVR AQMEKGVTGE SGYPVVDIRV TLFDGKAHSV DSSDFAFQMA GALALRDAAA ATKINLLEPV DDVTIVV PD DLVGTIMGDL SGRRGRVLGT DKHDADRTVI KAEIPEVELV RYAIDLRSMS HGAGQFRRSF ARYEPMP

UniProtKB: Elongation factor G-like protein

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Macromolecule #8: Small ribosomal subunit protein uS3

MacromoleculeName: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 23.61715 KDa
SequenceString: GQKINPHGFR LGITTEWKSR WYADKQYKDY VKEDVAIRKL LATGLERAGI ADVEIERTRD RVRVDIHTAR PGIVIGRRGT EADRIRADL EKLTGKQVQL NILEVKNPES QAQLVAQGVA EQLSNRVAFR RAMRKAIQSA MRQPNVKGIR VQCSGRLGGA E MSRSEFYR ...String:
GQKINPHGFR LGITTEWKSR WYADKQYKDY VKEDVAIRKL LATGLERAGI ADVEIERTRD RVRVDIHTAR PGIVIGRRGT EADRIRADL EKLTGKQVQL NILEVKNPES QAQLVAQGVA EQLSNRVAFR RAMRKAIQSA MRQPNVKGIR VQCSGRLGGA E MSRSEFYR EGRVPLHTLR ADIDYGLYEA KTTFGRIGVK VWIYKGDIVG

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #9: Small ribosomal subunit protein uS4

MacromoleculeName: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 23.28459 KDa
SequenceString: ARYTGPATRK SRRLGVDLVG GDQSFEKRPY PPGQHGRARI KESEYRQQLQ EKQKARFSYG VMEKQFRRYY EEANRQPGKT GDNLLRILE SRLDNVVYRA GLARTRRMAR QLVSHGHFLV NGVKVDIPSY RVSQYDIIDV KEKSLNTLPF QIARETAGER P IPSWLQVV ...String:
ARYTGPATRK SRRLGVDLVG GDQSFEKRPY PPGQHGRARI KESEYRQQLQ EKQKARFSYG VMEKQFRRYY EEANRQPGKT GDNLLRILE SRLDNVVYRA GLARTRRMAR QLVSHGHFLV NGVKVDIPSY RVSQYDIIDV KEKSLNTLPF QIARETAGER P IPSWLQVV GERQRILVHQ LPERAQIDVP LTEQLIVELY SK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #10: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 18.481439 KDa
SequenceString:
NYIERVVSIN RVSKVVKGGR RFSFTALVIV GDGKGMVGVG YGKAKEVPAA IAKGVEEARK NFFRVPLIGS TITHPVQGEA AAGVVMLRP ASPGTGVIAG GAARAVLECA GVHDILAKSL GSDNAINVVH ATVAALKLLQ RPEEVAARRG LPIEDVAPAG M LKARRESE ALAAAAAREG SA

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #11: Small ribosomal subunit protein bS6

MacromoleculeName: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.991637 KDa
SequenceString:
MRPYEIMVIL DPTLDERTVA PSLETFLNVI RKDGGTVDKV DIWGRRRLAY EIAKHAEGIY AVIDVKAEPA TVSELDRQLN LNESVLRTK VLRTDKH

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #12: Small ribosomal subunit protein uS7

MacromoleculeName: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 17.52918 KDa
SequenceString:
PRKGPAPKRP LVNDPVYGSQ LVTQLVNKVL LEGKKSLAER IVYGALEQAR EKTGTDPVVT LKRALDNVKP ALEVRSRRVG GATYQVPVE VRPDRSTTLA LRWLVNFSRQ RREKTMVERL ANEILDASNG LGASVKRRED THKMAEANRA FAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #13: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.361442 KDa
SequenceString:
TMTDPIADFL TRLRNANSAY HDEVTLPHSK LKANIAEILK REGYISDYRT EDARVGKSLV VQLKYGPSRE RSIAGLRRVS KPGLRVYAK STNLPRVLGG LGVAIISTSS GLLTDRQAAR QGVGGEVLAY VW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #14: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.093392 KDa
SequenceString:
PIQTVGRRKE AVVRVRLVPG TGQFNLDGRT LENYFPNKVH QQLIKAPLVT VDRVDQFDIY AHLDGGGPSG QAGALRLAIA RALILVQPE DRPALKKAGF LTRDPRAIER KKYGLKKARK APQYSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #15: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 11.252039 KDa
SequenceString:
GQKIRIRLKA YDHEAIDASA RKIVETVTRT GASVVGPVPL PTEKNVYCVI RSPHKYKDSR EHFEMRTHKR LIDILDPTPK TVDALMRID LPASVDVNIQ

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #16: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.151729 KDa
SequenceString:
KNVPHGAAHI KSTFNNTIVS ITDPQGNVIA WASSGHVGFK GSRKSTPFAA QLAAENAARK AQEHGVKKVD VFVKGPGSGR ETAIRSLQA AGLEVGTISD VTPQPHNGCR PPKRRR

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #17: Small ribosomal subunit protein uS12

MacromoleculeName: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.678093 KDa
SequenceString:
PTIQQLVRKG RRDKIAKVKT AALKGSPQRR GVCTRVYTTT PKKPNSALRK VARVKLTSQV EVTAYIPGEG HNLQEHSMVL VRGGRVKDL PGVRYKIIRG SLDTQGVKNR KQARSRYGAK KEK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #18: Small ribosomal subunit protein uS13

MacromoleculeName: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.374481 KDa
SequenceString:
ARLVGVDLPR DKRMEIALTY IYGIGRTRSN EILAATGIDK NMRTKDLTDD QVTVLRDYIE GNLKVEGDLR REVQADIRRK IEIGCYQGL RHRRGLPVRG QRTKTNARTR KGPKRTI

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #19: Small ribosomal subunit protein uS14B

MacromoleculeName: Small ribosomal subunit protein uS14B / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.845213 KDa
SequenceString:
AKKALVHKAN KKPKFAVRAY TRCNKCGRPH SVYRKFGLCR ICLREMAHAG ELPGVQKSSW

UniProtKB: Small ribosomal subunit protein uS14B

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Macromolecule #20: Small ribosomal subunit protein uS15

MacromoleculeName: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.2369 KDa
SequenceString:
ALTAEQKKEI LGQYGLHDTD TGSPEAQVAL LTKRIQDLTE HLKVHKHDHH SRRGLLLLVG RRRRLLKYVA QVDVARYRSL IERLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #21: Small ribosomal subunit protein bS16

MacromoleculeName: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.635652 KDa
SequenceString:
AVKIKLTRLG KIRNPQYRII VADARTRRDG RAIEVIGRYH PKEEPSLIQI DSERAQYWLG VGAQPTEPVL ALLKITGDWQ KFKGLPGAE GTLKVKEPKP SKLDLFNAAL AEAE

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #22: Small ribosomal subunit protein uS17

MacromoleculeName: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.680461 KDa
SequenceString:
QKGPKYTPAA EKPRGRRKTA IGYVVSDKMQ KTIVVELEDR KSHPLYGKII RTTKKVKAHD ENGEAGIGDR VSLMETRPLS ATKRWRLVE ILEKA

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #23: Small ribosomal subunit protein bS18B

MacromoleculeName: Small ribosomal subunit protein bS18B / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 7.353585 KDa
SequenceString:
TRKCVFCSKK GQTIDYKDTA LLRTYISERG KIRARRVTGN CVQHQRDIAV AVKNAREVAL LPFGS

UniProtKB: Small ribosomal subunit protein bS18B

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Macromolecule #24: Small ribosomal subunit protein uS19

MacromoleculeName: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.379874 KDa
SequenceString:
PRSLKKGPFV DDHLLKKVDV QNEKNTKQVI KTWSRRSTII PDFIGHTFAV HDGRKHVPVF VTEAMVGHKL GEFAPTRTFK GH

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #25: Small ribosomal subunit protein bS20

MacromoleculeName: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.424908 KDa
SequenceString:
ANIKSQIKRI RTNERRRLRN QSVKSSLRTA IRGFREAVDA GDKDKASELL HATSRKLDKA ASKGVIHPNQ AANKKSALAL ALNKL

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #26: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.748448 KDa
SequenceString:
ATITDPRDII LAPVISEKSY GLIEDNVYTF VVHPDSNKTQ IKIAIEKIFD VKVDSVNTAN RQGKRKRTRT GFGKRKSTKR AIVKLAAGS KPIDLFGA

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #27: Small ribosomal subunit protein uS2

MacromoleculeName: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 25.576547 KDa
SequenceString: MAVVTMKQLL DSGAHFGHQT RRWNPKMKRF IFTDRNGIYI IDLQQTLTYI DKAYEFVKET VAHGGTVLFV GTKKQAQESI AEEATRVGM PYVNQRWLGG MLTNFSTVHK RLQRLKELEA MEQTGGFEGR TKKEILMLTR EKNKLERSLG GIRDMQKVPS A VWVVDTNK ...String:
MAVVTMKQLL DSGAHFGHQT RRWNPKMKRF IFTDRNGIYI IDLQQTLTYI DKAYEFVKET VAHGGTVLFV GTKKQAQESI AEEATRVGM PYVNQRWLGG MLTNFSTVHK RLQRLKELEA MEQTGGFEGR TKKEILMLTR EKNKLERSLG GIRDMQKVPS A VWVVDTNK EHIAVGEARK LGIPVIAILD TNCDPDVVDY PIPGNDDAIR SAALLTKVIA SAVAEGLQAR

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #28: Large ribosomal subunit protein bL25

MacromoleculeName: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 20.331725 KDa
SequenceString: TANIPNKLTA NVRTRTGKGA SRQARRDGKV PAVLYGHGTD PQHLELNARD FAAVLRSHGT NAILTLDIEG TEQLALTKAL DVHPIRRNI QHADLLVVQR GEKVTVEVTV LVEGDATPGT LVTQDANTIE IEAEALSIPE QLTVSVEGVE AGTQITAGQI S LPEGVNLI ...String:
TANIPNKLTA NVRTRTGKGA SRQARRDGKV PAVLYGHGTD PQHLELNARD FAAVLRSHGT NAILTLDIEG TEQLALTKAL DVHPIRRNI QHADLLVVQR GEKVTVEVTV LVEGDATPGT LVTQDANTIE IEAEALSIPE QLTVSVEGVE AGTQITAGQI S LPEGVNLI SDPELLVVNV VEAPSAEALE EEGA

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #29: Large ribosomal subunit protein bL27

MacromoleculeName: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 8.322479 KDa
SequenceString:
SSSRNGRDSA AQRLGVKRFG GQVVKAGEIL VRQRGTHFHP GVNVGRGGDD TLFALAPGAV EFGAKRGRKT VNIVPVARP

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #30: Large ribosomal subunit protein bL28

MacromoleculeName: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.75982 KDa
SequenceString:
AAVCDICGKG PGFGKSVSHS HRRTSRRWNP NIQPVRAVTR PGGNKQRINA CTSCIKAGKV SRA

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #31: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 7.590576 KDa
SequenceString:
GTTPGELREL TDDELKDKLR ESKEELFNLR FQMATGQLSN NRRLRTVRQE IARVYTVLRE RELG

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #32: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.761772 KDa
SequenceString:
AELKITQVRS TIGARWKQRE SLRTLGLKKI RQSVVREDNA QTRGLINTVH HLVEVEEVG

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #33: Large ribosomal subunit protein bL32

MacromoleculeName: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.050149 KDa
SequenceString:
AVPKRRMSRA NTRSRRAQWK AEAPGLVTVS VAGQQRKVPR RLLKAARLGL VDLD

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #34: Large ribosomal subunit protein bL33A

MacromoleculeName: Large ribosomal subunit protein bL33A / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 5.833775 KDa
SequenceString:
DVRPKITLAC EVCKHRNYIT KKNRRNDPDR LEIKKFCPNC GTHQPHKES

UniProtKB: Large ribosomal subunit protein bL33A

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Macromolecule #35: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 5.391384 KDa
SequenceString:
AKGKRTFQPN NRRRARVHGF RLRMRTRAGR AIVANRRSKG RRALTA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #36: Large ribosomal subunit protein bL35

MacromoleculeName: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 7.16731 KDa
SequenceString:
PKAKTHSGAS KRFRRTGTGK IVRQKANRRH LLEHKPTKRT RRLDGRTTVS AADNSRINKL LNG

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #37: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 4.341277 KDa
SequenceString:
MKVNPSVKPI CDKCRVIRRH GRVMVICSDP RHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #38: Large ribosomal subunit protein bL31

MacromoleculeName: Large ribosomal subunit protein bL31 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 5.257952 KDa
SequenceString:
MKTGIHPEYV DTTVQCGCGH SFTTRSTKQS GTIVVEVCSQ CHPFYTGK

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #39: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 30.008379 KDa
SequenceString: GIRKYKPTTP GRRGASVSDF AEITRSTPEK SLVRPLHGKG GRNAHGRITT RHKGGGHKRA YRVIDFRRHD KDGVNAKVAH IEYDPNRTA NIALLHYLDG EKRYIIAPQG LKQGDVIESG ANADIKPGNN LPLRNIPAGT VIHAVELRPG GGAKLARSAG V SIQLLGKE ...String:
GIRKYKPTTP GRRGASVSDF AEITRSTPEK SLVRPLHGKG GRNAHGRITT RHKGGGHKRA YRVIDFRRHD KDGVNAKVAH IEYDPNRTA NIALLHYLDG EKRYIIAPQG LKQGDVIESG ANADIKPGNN LPLRNIPAGT VIHAVELRPG GGAKLARSAG V SIQLLGKE GTYAALRMPS GEIRRVDVRC RATVGEVGNA EQSNINWGKA GRMRWKGKRP TVRGVVMNPV DHPHGGGEGK TS GGRHPVS PWGKPEGRTR KPNKPSDKLI VRRRRTGK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #40: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 22.621902 KDa
SequenceString: ARKGILGTKL GMTQVFDENN KVVPVTVVKA GPNVVTRIRT TERDGYSAVQ LAYGEISPRK VIKPVAGQFA AAGVNPRRHV AELRLDDEA AVAEYEVGQE LTAEIFSDGA YVDVTGTSKG KGFAGTMKRH GFRGQGAAHG AQAVHRRPGS IGGCATPGRV F KGTRMSGR ...String:
ARKGILGTKL GMTQVFDENN KVVPVTVVKA GPNVVTRIRT TERDGYSAVQ LAYGEISPRK VIKPVAGQFA AAGVNPRRHV AELRLDDEA AVAEYEVGQE LTAEIFSDGA YVDVTGTSKG KGFAGTMKRH GFRGQGAAHG AQAVHRRPGS IGGCATPGRV F KGTRMSGR MGNDRVTTQN LKVHKVDAEN GVLLIKGAIP GRNGGLVVVR SAIKRG

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #41: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 22.346301 KDa
SequenceString: TLKVDVKTPA GKTDGSVELP AELFDVEPNI ALMHQVVTAQ LAAKRQGTHS TKTRGEVSGG GKKPYRQKGT GRARQGSTRA PQFTGGGTV HGPKPRDYSQ RTPKKMIAAA LRGALSDRAR NDRIHAVTEL VEGQTPSTKS AKTFLGTLTE NKKVLVVIGR T DEVGAKSV ...String:
TLKVDVKTPA GKTDGSVELP AELFDVEPNI ALMHQVVTAQ LAAKRQGTHS TKTRGEVSGG GKKPYRQKGT GRARQGSTRA PQFTGGGTV HGPKPRDYSQ RTPKKMIAAA LRGALSDRAR NDRIHAVTEL VEGQTPSTKS AKTFLGTLTE NKKVLVVIGR T DEVGAKSV RNLPGVHVIS PDQLNTYDVL NADDVVFSVE ALNAYISANS K

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #42: Large ribosomal subunit protein uL5

MacromoleculeName: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 20.588701 KDa
SequenceString:
KALPRLKQRY REEIREALQQ EFNYANVMQI PGVVKVVVNM GVGDAARDAK LINGAINDLA LITGQKPEVR RARKSIAQFK LREGMPIGA RVTLRGDRMW EFLDRLISIA LPRIRDFRGL SPKQFDGTGN YTFGLNEQSM FHEIDVDSID RPRGMDITVV T TATNDAEG RALLRALGFP FKEN

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #43: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 19.165934 KDa
SequenceString:
SRIGKQPVPV PSGVDVTING QNLSVKGPKG TLTLDVAEPI SVSRAEDGAI VVTRPDDERR SRSLHGLSRT LIANLVTGVT EGYTQKMEI FGVGYRVQLK GQNLEFALGY SHPVLIEAPE GITFAVESPT KFSVSGIDKQ KVGQISAVIR RLRRPDPYKG K GVRYEGEQ IRRKVGKT

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #44: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 15.949253 KDa
SequenceString:
MKLILTAEVE HLGAAGDTVE VKDGYGRNYL LPRGLAIVAS RGAERQAEEI RRARESKVIR DIEHANELKT ALEGLGDVTL SVNAAGDTG KLFGSVTAAD VVNAIKKAGG PNLDKRTVQL AKAHIKSVGT HPVTVKLHTG VEAKVSLNVV AQ

UniProtKB: Large ribosomal subunit protein bL9

+
Macromolecule #45: Large ribosomal subunit protein uL10

MacromoleculeName: Large ribosomal subunit protein uL10 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.095021 KDa
SequenceString:
MAKADKATAV ADIAEQFKAS TATVVTEYRG LTVANLAELR RALGDSATYT VAKNTLVKRA ASEAGIEGLD ELFAGPTAIA FVKGEAVDA AKAIKKFAKD NKALVIKGGY MDGKALSVAD VEKIADL

UniProtKB: Large ribosomal subunit protein uL10

+
Macromolecule #46: Large ribosomal subunit protein uL11

MacromoleculeName: Large ribosomal subunit protein uL11 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.109185 KDa
SequenceString:
LIKLQIQAGQ ANPAPPVGPA LGQHGVNIME FCKAYNAATE SQRGNVIPVE ITVYEDRSFT FALKTPPAAK LLLKAAGVQK GSGEPHKTK VAKVTWDQVR EIAETKKADL NANDIDAAAK IIAGTARSMG ITVE

UniProtKB: Large ribosomal subunit protein uL11

+
Macromolecule #47: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 16.015493 KDa
SequenceString:
PTYTPKAGDT TRSWYVIDAS DVVLGRLASA AATLLRGKHK PTFTPNVDGG DFVIVINADK IAVSGDKLTK KFAYRHSGYP GGLRKRTIG ELLEKHPTRV VENAIIGMLP HNKLGRQIQK KLKVYAGPDH PHAAQQPIPF EIKQVAQ

UniProtKB: Large ribosomal subunit protein uL13

+
Macromolecule #48: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.400625 KDa
SequenceString:
MIQQESRLKV ADNTGAKEIL CIRVLGGSSR RYAGIGDVIV ATVKDAIPGG NVKRGDVVKA VVVRTVKERR RADGSYIKFD ENAAVIIKN DNDPRGTRIF GPVGRELREK KFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

+
Macromolecule #49: Large ribosomal subunit protein uL15

MacromoleculeName: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 15.383968 KDa
SequenceString:
VIKLHDLKPA PGEKKAKTRV GRGEGSKGKT AGRGTKGTKA RKNVPVMFEG GQMPIHMRLP KLKGFKNRFR TEYQVVNVGD INKAFPQGG TVGVDELVAK GLVRKNSLVK VLGDGKLTVK VDVTANKFSG SAREAITAAG GSATEL

UniProtKB: Large ribosomal subunit protein uL15

+
Macromolecule #50: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 15.498937 KDa
SequenceString:
MLIPRKVKHR KQHHPEQRGI ASGGTSVSFG DYGIQALEHA YITNRQIESA RIAINRHIKR GGKVWINIFP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVLFELSYP DEKTARDALT RAIHKLPIKA RIVTREE

UniProtKB: Large ribosomal subunit protein uL16

+
Macromolecule #51: Large ribosomal subunit protein bL17

MacromoleculeName: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.210346 KDa
SequenceString:
PKPTKGPRLG GSSSHQSALL ANLATSLFEH GRIKTTEPKA RALRPYAEKL ITHAKKGALH NRREVMKKIR DKDVVHTLFA EIGPFYADR NGGYTRIIKV ENRKGDNAPM AVIELVREK

UniProtKB: Large ribosomal subunit protein bL17

+
Macromolecule #52: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.580531 KDa
SequenceString:
AHKPVGQNIS EVRRNARLRR HARLRKKVAG TAEVPRLVVN RSARHIHVQL VNDLNGTTLA AASSIEADVR AIDGDKKAHS VRVGQLIAE RAKAAGVETV VFDRGGYTYG GRIAALADAA REAGLKF

UniProtKB: Large ribosomal subunit protein uL18

+
Macromolecule #53: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.892806 KDa
SequenceString:
MNTLDFVDQA SLRDDIPTFS PGDTVNVHVK VIEGSKERIQ VFKGVVIRRQ GGGISETFTV RKESYGVGVE RTFPVHSPNI DHIDVLTRG DVRRAKLYYL RELRGKKAKI KEKR

UniProtKB: Large ribosomal subunit protein bL19

+
Macromolecule #54: Large ribosomal subunit protein bL20

MacromoleculeName: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.035175 KDa
SequenceString:
ARVKRALNAQ KKRRTVLKAS KGYRGQRSRL YRKAKEQQLH SLTYAYRDRR ARKGEFRKLW ISRINAAARA NDITYNRLIQ GLKAAGVEV DRKNLAELAV SDPAAFTALV DVARAALPED VNAPS

UniProtKB: Large ribosomal subunit protein bL20

+
Macromolecule #55: Large ribosomal subunit protein bL21

MacromoleculeName: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.72444 KDa
SequenceString:
TYAIVKTGGK QYKVAAGDVV KVEKLDSEPG ASVSLPVALV VDGANVTSKA DDLAKVAVTA EVLEHTKGPK IRIHKFKNKT GYHKRQGHR QQLTVLKVTG I

UniProtKB: Large ribosomal subunit protein bL21

+
Macromolecule #56: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.408192 KDa
SequenceString:
EFPSATAKAR YVRVSATKAR RVIDLVRGKS VEEALDILRW APQAASEPVA KVIASAAANA QNNEGLDPST LVVATVYADE GPTAKRIRP RAQGRAFRIR KRTSHITVIV ESRPP

UniProtKB: Large ribosomal subunit protein uL22

+
Macromolecule #57: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 11.228946 KDa
SequenceString:
MKVHKGDTVL VISGKDKGAK GKVLVAYPDR NKVLVEGVNR IKKHTAVSAN ERGASSGGIV TQEAPIHVSN VMVVDSDGKP TRVGYRIDD ETGKKVRIAK TNGKDI

UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #59: PHENYLALANINE

MacromoleculeName: PHENYLALANINE / type: ligand / ID: 59 / Number of copies: 1 / Formula: PHE
Molecular weightTheoretical: 165.189 Da
Chemical component information

ChemComp-PHE:
PHENYLALANINE

+
Macromolecule #60: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 60 / Number of copies: 1 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

+
Macromolecule #61: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 61 / Number of copies: 12 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #62: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 62 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.8
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 54.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.3000000000000003 µm / Nominal defocus min: 1.8 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5o61

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 19431
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: ANGULAR RECONSTITUTION

-
Atomic model buiding 1

Initial modelPDB ID:

5o61


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-9k0z:
EF-G2 bound 70S ribosome complex of M. smegmatis

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