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基本情報
登録情報 | データベース: EMDB / ID: EMD-5968 | |||||||||
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タイトル | Single particle EM reveals plasticity of interactions between the adenovirus penton base and integrin alphaV-beta3 | |||||||||
![]() | Reconstruction of the human alphaV-beta3 integrin in complex with the monomeric RGD-loop containing insertion domain of the adenovirus 9 penton base subunit | |||||||||
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![]() | adenovirus / integrin / virus infection / electron microscopy / fluorescence correlation microscopy | |||||||||
機能・相同性 | ![]() T=25 icosahedral viral capsid / integrin alphav-beta6 complex / integrin alphav-beta8 complex / transforming growth factor beta production / negative regulation of entry of bacterium into host cell / integrin alphav-beta5 complex / : / opsonin binding / integrin alphav-beta1 complex / Cross-presentation of particulate exogenous antigens (phagosomes) ...T=25 icosahedral viral capsid / integrin alphav-beta6 complex / integrin alphav-beta8 complex / transforming growth factor beta production / negative regulation of entry of bacterium into host cell / integrin alphav-beta5 complex / : / opsonin binding / integrin alphav-beta1 complex / Cross-presentation of particulate exogenous antigens (phagosomes) / extracellular matrix protein binding / Laminin interactions / integrin alphav-beta3 complex / negative regulation of lipoprotein metabolic process / alphav-beta3 integrin-PKCalpha complex / entry into host cell by a symbiont-containing vacuole / alphav-beta3 integrin-HMGB1 complex / negative regulation of lipid transport / negative regulation of low-density lipoprotein receptor activity / regulation of phagocytosis / Elastic fibre formation / alphav-beta3 integrin-IGF-1-IGF1R complex / transforming growth factor beta binding / filopodium membrane / positive regulation of small GTPase mediated signal transduction / extracellular matrix binding / apolipoprotein A-I-mediated signaling pathway / apoptotic cell clearance / wound healing, spreading of epidermal cells / heterotypic cell-cell adhesion / integrin complex / Molecules associated with elastic fibres / positive regulation of intracellular signal transduction / microvillus membrane / negative chemotaxis / cell adhesion mediated by integrin / Syndecan interactions / cell-substrate adhesion / endodermal cell differentiation / positive regulation of osteoblast proliferation / TGF-beta receptor signaling activates SMADs / PECAM1 interactions / lamellipodium membrane / negative regulation of macrophage derived foam cell differentiation / negative regulation of lipid storage / fibronectin binding / positive regulation of cell adhesion / ECM proteoglycans / voltage-gated calcium channel activity / vasculogenesis / Integrin cell surface interactions / coreceptor activity / specific granule membrane / extrinsic apoptotic signaling pathway in absence of ligand / ERK1 and ERK2 cascade / phagocytic vesicle / substrate adhesion-dependent cell spreading / cell-matrix adhesion / Signal transduction by L1 / integrin-mediated signaling pathway / negative regulation of extrinsic apoptotic signaling pathway / calcium ion transmembrane transport / protein kinase C binding / endocytosis involved in viral entry into host cell / ruffle membrane / VEGFA-VEGFR2 Pathway / cell-cell adhesion / cell migration / integrin binding / virus receptor activity / positive regulation of cytosolic calcium ion concentration / angiogenesis / protease binding / cell adhesion / positive regulation of cell migration / symbiont entry into host cell / external side of plasma membrane / focal adhesion / positive regulation of cell population proliferation / Neutrophil degranulation / cell surface / extracellular exosome / membrane / metal ion binding / plasma membrane / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / ネガティブ染色法 / 解像度: 54.0 Å | |||||||||
![]() | Veesler D / Cupelli K / Burger M / Graber P / Stehle T / Johnson JE | |||||||||
![]() | ![]() タイトル: Single-particle EM reveals plasticity of interactions between the adenovirus penton base and integrin αVβ3. 著者: David Veesler / Karolina Cupelli / Markus Burger / Peter Gräber / Thilo Stehle / John E Johnson / ![]() ![]() 要旨: Human adenoviruses are double-stranded DNA viruses responsible for numerous infections, some of which can be fatal. Furthermore, adenoviruses are currently used in clinical trials as vectors for gene ...Human adenoviruses are double-stranded DNA viruses responsible for numerous infections, some of which can be fatal. Furthermore, adenoviruses are currently used in clinical trials as vectors for gene therapy applications. Although initial binding of adenoviruses to host attachment receptors has been extensively characterized, the interactions with the entry receptor (integrins) remain poorly understood at the structural level. We characterized the interactions between the adenovirus 9 penton base subunit and αVβ3 integrin using fluorescence correlation spectroscopy and single-particle electron microscopy to understand the mechanisms underlying virus internalization and infection. Our results indicate that the penton base subunit can bind integrins with high affinity and in several different orientations. These outcomes correlate with the requirement of the pentameric penton base to simultaneously bind several integrins to enable their clustering and promote virus entry into the host cell. | |||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
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マップデータ | ![]() | 2.1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 10.8 KB 10.8 KB | 表示 表示 | ![]() |
画像 | ![]() ![]() | 18.6 KB 2.2 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 78.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 77.5 KB | 表示 | |
XML形式データ | ![]() | 495 B | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 5955C ![]() 5956C ![]() 5957C ![]() 5958C ![]() 5959C ![]() 5960C ![]() 5961C ![]() 5962C ![]() 5963C ![]() 5964C ![]() 5965C ![]() 5966C ![]() 5967C ![]() 5969C ![]() 5970C ![]() 5971C ![]() 5972C ![]() 5973C C: 同じ文献を引用 ( |
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類似構造データ |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Reconstruction of the human alphaV-beta3 integrin in complex with the monomeric RGD-loop containing insertion domain of the adenovirus 9 penton base subunit | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 4.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Human alphaV-beta3 integrin ectodomain in complex with the monome...
全体 | 名称: Human alphaV-beta3 integrin ectodomain in complex with the monomeric RGD-loop containing insertion domain of the adenovirus 9 penton base subunit |
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要素 |
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-超分子 #1000: Human alphaV-beta3 integrin ectodomain in complex with the monome...
超分子 | 名称: Human alphaV-beta3 integrin ectodomain in complex with the monomeric RGD-loop containing insertion domain of the adenovirus 9 penton base subunit タイプ: sample / ID: 1000 / 集合状態: heterotrimer / Number unique components: 2 |
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分子量 | 理論値: 210 KDa |
-分子 #1: alphaV-beta3 integrin ectodomain
分子 | 名称: alphaV-beta3 integrin ectodomain / タイプ: protein_or_peptide / ID: 1 / 詳細: Second UniProt identifier: P05106 / コピー数: 1 / 集合状態: heterodimer / 組換発現: Yes |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 180 KDa |
組換発現 | 生物種: ![]() ![]() 組換細胞: Sf9 / 組換プラスミド: pFastBacDual |
配列 | UniProtKB: Integrin alpha-V |
-分子 #2: adenovirus 9 penton base
分子 | 名称: adenovirus 9 penton base / タイプ: protein_or_peptide / ID: 2 詳細: Only the monomeric RGD-loop containing insertion domain of the adenovirus 9 penton base subunit is present in the construct used (residues 116 to 360). コピー数: 1 / 集合状態: Monomer / 組換発現: Yes |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 30 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | UniProtKB: Penton base |
-実験情報
-構造解析
手法 | ネガティブ染色法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 / 詳細: 10 mM Tris, 150 mM NaCl, 2 mM MnCl2 |
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染色 | タイプ: NEGATIVE / 詳細: 2% uranyl formate |
グリッド | 詳細: C-flat 2/0.5 grids overlaid with a thin layer of carbon |
凍結 | 凍結剤: NONE / 装置: OTHER |
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電子顕微鏡法
顕微鏡 | FEI TECNAI 12 |
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日付 | 2013年5月30日 |
撮影 | カテゴリ: CCD フィルム・検出器のモデル: TVIPS TEMCAM-F416 (4k x 4k) 実像数: 1600 / 平均電子線量: 24 e/Å2 |
電子線 | 加速電圧: 120 kV / 電子線源: LAB6 |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.2 mm / 最大 デフォーカス(公称値): 2.8 µm / 最小 デフォーカス(公称値): 0.1 µm / 倍率(公称値): 76097 |
試料ステージ | 試料ホルダーモデル: OTHER |
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画像解析
詳細 | Random conical tilt reconstruction |
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最終 再構成 | アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 54.0 Å / 解像度の算出法: OTHER / ソフトウェア - 名称: Spider / 使用した粒子像数: 451 |
最終 2次元分類 | クラス数: 1 |