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- EMDB-5375: Direct electron detection yields cryo-EM reconstructions at resol... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-5375 | |||||||||
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Title | Direct electron detection yields cryo-EM reconstructions at resolutions beyond .75 Nyquist frequency | |||||||||
![]() | Icosahedral reconstruction of bacteriophage P22 | |||||||||
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![]() | Cryo-EM / Electron cryo-microscopy / Direct detection device / Active pixel sensor / CMOS detector / Nyquist frequency | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 8.5 Å | |||||||||
![]() | Bammes BE / Rochat RH / Jakana J / Chen D / Chiu W | |||||||||
![]() | ![]() Title: Direct electron detection yields cryo-EM reconstructions at resolutions beyond 3/4 Nyquist frequency. Authors: Benjamin E Bammes / Ryan H Rochat / Joanita Jakana / Dong-Hua Chen / Wah Chiu / ![]() Abstract: One limitation in electron cryo-microscopy (cryo-EM) is the inability to recover high-resolution signal from the image-recording media at the full-resolution limit of the transmission electron ...One limitation in electron cryo-microscopy (cryo-EM) is the inability to recover high-resolution signal from the image-recording media at the full-resolution limit of the transmission electron microscope. Direct electron detection using CMOS-based sensors for digitally recording images has the potential to alleviate this shortcoming. Here, we report a practical performance evaluation of a Direct Detection Device (DDD®) for biological cryo-EM at two different microscope voltages: 200 and 300 kV. Our DDD images of amorphous and graphitized carbon show strong per-pixel contrast with image resolution near the theoretical sampling limit of the data. Single-particle reconstructions of two frozen-hydrated bacteriophages, P22 and ε15, establish that the DDD is capable of recording usable signal for 3D reconstructions at about 4/5 of the Nyquist frequency, which is a vast improvement over the performance of conventional imaging media. We anticipate the unparalleled performance of this digital recording device will dramatically benefit cryo-EM for routine tomographic and single-particle structural determination of biological specimens. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 196.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.9 KB 9.9 KB | Display Display | ![]() |
Images | ![]() | 116.4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 77.6 KB | Display | ![]() |
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Full document | ![]() | 76.7 KB | Display | |
Data in XML | ![]() | 494 B | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Icosahedral reconstruction of bacteriophage P22 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.48 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Bacteriophage P22 procapsid
Entire | Name: Bacteriophage P22 procapsid |
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Components |
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-Supramolecule #1000: Bacteriophage P22 procapsid
Supramolecule | Name: Bacteriophage P22 procapsid / type: sample / ID: 1000 / Number unique components: 1 |
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-Supramolecule #1: Enterobacteria phage P22
Supramolecule | Name: Enterobacteria phage P22 / type: virus / ID: 1 / Name.synonym: P22 / NCBI-ID: 10754 / Sci species name: Enterobacteria phage P22 / Database: NCBI / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: Yes / Syn species name: P22 |
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Host (natural) | Organism: ![]() |
Virus shell | Shell ID: 1 / Name: Procapsid / Diameter: 525 Å / T number (triangulation number): 7 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 7.6 / Details: 50 mM Tris pH 7.6, 25 mM NaCl, 2mM EDTA |
Grid | Details: 400 mesh copper grid |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 170 K / Instrument: OTHER / Details: Vitrification instrument: Vitrobot / Method: Blot for 2 seconds before plunging |
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Electron microscopy
Microscope | JEOL 2010F |
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Temperature | Min: 100 K / Max: 100 K / Average: 100 K |
Alignment procedure | Legacy - Astigmatism: objective lens astigmatism was corrected at 100,000 times magnification |
Date | Jun 10, 2010 |
Image recording | Category: CCD / Film or detector model: DIRECT ELECTRON DE-12 (4k x 3k) / Digitization - Sampling interval: 6 µm / Number real images: 200 / Average electron dose: 17 e/Å2 Details: Images were collected on a CMOS type detector DE-12 from Direct Electron |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 17200 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 5.0 µm / Nominal defocus min: 2.0 µm / Nominal magnification: 15000 |
Sample stage | Specimen holder: Side Entry / Specimen holder model: GATAN LIQUID NITROGEN |
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Image processing
CTF correction | Details: Each Micrograph |
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Final reconstruction | Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 8.5 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: MPSA Details: Resolution was calculated to be 8.5 Angstroms at 0.5 FSC and 7.3 Angstroms at 0.143 FSC as compared against the 3.8 Angstrom reconstruction of P22. Number images used: 7500 |