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- EMDB-50014: Structure of the small subunit of the flowering plant mitoribosom... -

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Basic information

Entry
Database: EMDB / ID: EMD-50014
TitleStructure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA
Map data
Sample
  • Complex: Small subunit of the flowering plant mitochondrial ribosome in presence of RsgA
    • Protein or peptide: x 35 types
    • RNA: x 1 types
  • Protein or peptide: x 1 types
  • Ligand: x 5 types
Keywordsmitochondria / mitoribosome / assembly factor / plant / RIBOSOME
Function / homology
Function and homology information


pollen tube development / plastid translation / gametophyte development / 3-hydroxyisobutyryl-CoA hydrolase / monocarboxylic acid metabolic process / embryo development ending in seed dormancy / cell communication / molybdenum ion binding / mitochondrial small ribosomal subunit / mitochondrial translation ...pollen tube development / plastid translation / gametophyte development / 3-hydroxyisobutyryl-CoA hydrolase / monocarboxylic acid metabolic process / embryo development ending in seed dormancy / cell communication / molybdenum ion binding / mitochondrial small ribosomal subunit / mitochondrial translation / mitochondrial membrane organization / plastid / catalytic activity / chloroplast / pyridoxal phosphate binding / small ribosomal subunit / small ribosomal subunit rRNA binding / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / GTP binding / mitochondrion / RNA binding / cytoplasm
Similarity search - Function
CID8-like protein, RNA recognition motif 1 / CID8-like protein, RNA recognition motif 2 / IMS import disulfide relay-system, CHCH-CHCH-like Cx9C / CHCH-CHCH-like Cx9C, IMS import disulfide relay-system, / MOSC, N-terminal beta barrel / MOSC N-terminal beta barrel domain / Ribosomal protein S3, mitochondrial, plant / : / Molybdenum cofactor sulfurase, C-terminal / : ...CID8-like protein, RNA recognition motif 1 / CID8-like protein, RNA recognition motif 2 / IMS import disulfide relay-system, CHCH-CHCH-like Cx9C / CHCH-CHCH-like Cx9C, IMS import disulfide relay-system, / MOSC, N-terminal beta barrel / MOSC N-terminal beta barrel domain / Ribosomal protein S3, mitochondrial, plant / : / Molybdenum cofactor sulfurase, C-terminal / : / MOSC domain / MOSC domain profile. / Ataxin-2, C-terminal / Ataxin-2 C-terminal region / 30S ribosomal protein S31, plant / Ribosomal protein S31e / : / Ribosome biogenesis GTPase RsgA / RsgA GTPase domain / RsgA GTPase / EngC GTPase domain profile. / Pentacotripeptide-repeat region of PRORP / Pentacotripeptide-repeat region of PRORP / : / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Ribosomal protein MRP10, mitochondrial / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase / Small ribosomal subunit protein mS41 SAM domain / IGR protein motif / IGR / Cysteine alpha-hairpin motif superfamily / Mitochondrial 28S ribosomal protein S34 / Mitochondrial 28S ribosomal protein S34 / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Pyruvate kinase-like, insert domain superfamily / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Pentatricopeptide repeat / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / 30S ribosomal protein / Coiled coil-helix-coiled coil-helix (CHCH) domain profile. / ClpP/crotonase-like domain superfamily / Ribosomal protein S21 / Ribosomal protein S21 / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / RNA-binding domain superfamily / Ribosomal protein S19, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S14, conserved site / Ribosomal protein S14 signature. / Ribosomal protein S2 signature 1. / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S10 / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / : / Ribosomal protein S2, conserved site / K homology domain superfamily, prokaryotic type / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S5 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal / Ribosomal protein S5, C-terminal / Ribosomal protein S5, N-terminal domain / Ribosomal protein S13, conserved site
Similarity search - Domain/homology
Small ribosomal subunit protein uS3m / Small ribosomal subunit protein bS18c / BnaC06g06470D protein / 30S ribosomal protein S16 / S5 DRBM domain-containing protein / Ribosomal protein S21 / Uncharacterized protein / 30S ribosomal protein S31, mitochondrial / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein uS10 domain-containing protein ...Small ribosomal subunit protein uS3m / Small ribosomal subunit protein bS18c / BnaC06g06470D protein / 30S ribosomal protein S16 / S5 DRBM domain-containing protein / Ribosomal protein S21 / Uncharacterized protein / 30S ribosomal protein S31, mitochondrial / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein uS10 domain-containing protein / Uncharacterized protein / Ribosomal protein S2 / EngC GTPase domain-containing protein / Uncharacterized protein / Small ribosomal subunit protein uS15c / Small ribosomal subunit protein mS41 SAM domain-containing protein / 40S ribosomal protein S15a / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS23 / Ribosomal protein S11 / Small ribosomal subunit protein uS17c / MOSC domain-containing protein / Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein / RRM domain-containing protein / Small ribosomal subunit protein mS38 / PROP1-like PPR domain-containing protein / Mitochondrial 28S ribosomal protein S34 / 3-hydroxyisobutyryl-CoA hydrolase / Uncharacterized protein / (rape) hypothetical protein / IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein / Small ribosomal subunit protein uS19m / Small ribosomal subunit protein uS12m / Ribosomal protein S4
Similarity search - Component
Biological speciesBrassica oleracea var. botrytis (cauliflower)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.27 Å
AuthorsWaltz F / Skaltsogiannis V / Giege P
Funding support Germany, Switzerland, 2 items
OrganizationGrant numberCountry
Alexander von Humboldt Foundation Germany
Swiss National Science Foundation210561 Switzerland
CitationJournal: Nat Commun / Year: 2025
Title: Structural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor.
Authors: Vasileios Skaltsogiannis / Philippe Wolff / Tan-Trung Nguyen / Nicolas Corre / David Pflieger / Todd Blevins / Yaser Hashem / Philippe Giegé / Florent Waltz /
Abstract: Ribosomes are key molecular machines that translate mRNA into proteins. Mitoribosomes are specific ribosomes found in mitochondria, which have been shown to be remarkably diverse across eukaryotic ...Ribosomes are key molecular machines that translate mRNA into proteins. Mitoribosomes are specific ribosomes found in mitochondria, which have been shown to be remarkably diverse across eukaryotic lineages. In plants, they possess unique features, including additional rRNA domains stabilized by plant-specific proteins. However, the structural specificities of plant mitoribosomes in translation state remained unknown. We used cryo-electron microscopy to provide a high-resolution structural characterization of the cauliflower mitoribosome, in translating and maturation states. The structure reveals the mitoribosome bound with a tRNA in the peptidyl site, along with a segment of mRNA and a nascent polypeptide. Moreover, using structural data, nanopore sequencing and mass spectrometry, we identify a set of 19 ribosomal RNA modifications. Additionally, we observe a late assembly intermediate of the small ribosomal subunit, in complex with the RsgA assembly factor. This reveals how a plant-specific extension of RsgA blocks the mRNA channel to prevent premature mRNA association before complete small subunit maturation. Our findings elucidate key aspects of translation in angiosperm plant mitochondria, revealing its distinct features compared to other eukaryotic lineages.
History
DepositionApr 2, 2024-
Header (metadata) releaseMar 18, 2026-
Map releaseMar 18, 2026-
UpdateMar 18, 2026-
Current statusMar 18, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_50014.png

Downloads & links

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Map

FileDownload / File: emd_50014.map.gz / Format: CCP4 / Size: 775.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
0.89 Å/pix.
x 588 pix.
= 524.878 Å		0.89 Å/pix.
x 588 pix.
= 524.878 Å		0.89 Å/pix.
x 588 pix.
= 524.878 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.89265 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.0377
Minimum - Maximum-0.5723365 - 1.0586492
Average (Standard dev.)-0.00063157093 (±0.023356847)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions588588588
Spacing588588588
CellA=B=C: 524.87823 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_50014_msk_1.map
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Half map: #2

Fileemd_50014_half_map_1.map
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Half map: #1

Fileemd_50014_half_map_2.map
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Sample components

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Entire : Small subunit of the flowering plant mitochondrial ribosome in pr...

EntireName: Small subunit of the flowering plant mitochondrial ribosome in presence of RsgA
Components
  • Complex: Small subunit of the flowering plant mitochondrial ribosome in presence of RsgA
    • Protein or peptide: Ribosomal protein S2
    • Protein or peptide: Small ribosomal subunit protein uS3m
    • Protein or peptide: Ribosomal protein S4
    • Protein or peptide: S5 DRBM domain-containing protein
    • Protein or peptide: bS6m
    • Protein or peptide: uS7m,Small ribosomal subunit protein uS7m,Small ribosomal subunit protein uS7m
    • Protein or peptide: 40S ribosomal protein S15a
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: Small ribosomal subunit protein uS10 domain-containing protein
    • Protein or peptide: Ribosomal protein S11
    • Protein or peptide: uS13m
    • Protein or peptide: Small ribosomal subunit protein uS14m
    • Protein or peptide: Small ribosomal subunit protein uS15c
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: Small ribosomal subunit protein uS17c
    • Protein or peptide: Small ribosomal subunit protein bS18c
    • Protein or peptide: Small ribosomal subunit protein uS19m
    • Protein or peptide: Ribosomal protein S21
    • Protein or peptide: 30S ribosomal protein S31, mitochondrial
    • Protein or peptide: Small ribosomal subunit protein mS23
    • Protein or peptide: mS26
    • Protein or peptide: Small ribosomal subunit protein mS29
    • Protein or peptide: BnaC06g06470D protein
    • Protein or peptide: MOSC domain-containing protein
    • Protein or peptide: Mitochondrial 28S ribosomal protein S34
    • Protein or peptide: EngC GTPase domain-containing protein
    • Protein or peptide: Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein
    • Protein or peptide: IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein
    • Protein or peptide: Small ribosomal subunit protein mS38
    • Protein or peptide: IGR domain-containing protein
    • Protein or peptide: mS45
    • Protein or peptide: mS47
    • Protein or peptide: (rape) hypothetical protein
    • Protein or peptide: PROP1-like PPR domain-containing protein
    • Protein or peptide: RRM domain-containing protein
    • RNA: 18S rRNA
  • Protein or peptide: Small ribosomal subunit protein uS12m
  • Ligand: POTASSIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: ZINC ION
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE

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Supramolecule #1: Small subunit of the flowering plant mitochondrial ribosome in pr...

SupramoleculeName: Small subunit of the flowering plant mitochondrial ribosome in presence of RsgA
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#10, #12-#37
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)

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Macromolecule #1: Ribosomal protein S2

MacromoleculeName: Ribosomal protein S2 / type: protein_or_peptide / ID: 1 / Details: GWHPBJSH046906 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 23.743545 KDa
SequenceString: MTLHGAVIQK LLNTGSHLGR RAAEHHFKQY AYGTRNGMTI IDSDKTLICL RSAASFVANL ASARGNIFFV NTNPLFDEIV ELTSRRIQG DAYNHNRSTN LWKMGGFLTN SYSPKKFRSR HKKLCFGPTT MPDCVVVFDA ERKSSVVLEA AKLQIPVVAI V DPNVPLEF ...String:
MTLHGAVIQK LLNTGSHLGR RAAEHHFKQY AYGTRNGMTI IDSDKTLICL RSAASFVANL ASARGNIFFV NTNPLFDEIV ELTSRRIQG DAYNHNRSTN LWKMGGFLTN SYSPKKFRSR HKKLCFGPTT MPDCVVVFDA ERKSSVVLEA AKLQIPVVAI V DPNVPLEF FEKITYPVPA RDSVKFVYLF CNVITKCFVA EQMKMGIKDG DLAA

UniProtKB: Ribosomal protein S2

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Macromolecule #2: Small ribosomal subunit protein uS3m

MacromoleculeName: Small ribosomal subunit protein uS3m / type: protein_or_peptide / ID: 2 / Details: GWHPBJSH000741 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 64.731312 KDa
SequenceString: MARKGNPISV RLGKNRSSDS SWFSDYYYGK FVYQDVNLRS YFGSIRPPTR LTFGFRLGRC ILLHFPKRTF IHFFLPRRPR RLKRREKTR PGKEKGRWWT TFGKAGPIGC LRDDTEEERN EVRGRGARKR VESIRLDDRK KQNEIRGWPK KKQRYGYHDR S PSIKKNLS ...String:
MARKGNPISV RLGKNRSSDS SWFSDYYYGK FVYQDVNLRS YFGSIRPPTR LTFGFRLGRC ILLHFPKRTF IHFFLPRRPR RLKRREKTR PGKEKGRWWT TFGKAGPIGC LRDDTEEERN EVRGRGARKR VESIRLDDRK KQNEIRGWPK KKQRYGYHDR S PSIKKNLS KLLRISGAFK HPKYAGVVND IAFLIENDDS FKKTKLFKFF FPKKSRSDGP TSYLRTLPAV RPSLNFLVMQ YF FNTKNQM NFDPVVVLNH FVAPGAAEPS TMGRANAQGR SLQKRIRSRI AFFVESLTSE KKCLAEAKNR LTHFIRLAND LRF AGTTKT TISLFPFFGA TFFFLRDGVG VYNNLDAREQ LLNQLRVKCW NLLGKDKVME LIEKFKNLGG IEELIKVIDM MIEI ILRKR GIPYRYNSYF YEVKKMRSFL SNRTNTKTLI ESVKIKSVYQ SASLIAQDIS FQLKNKRRSF HSIFAKIVKE IPKRV EGIR ICFSGRLKDA AEKAQTKCYK HRKTSCNVFN QKIDYAPVEV STRYGILGVK VWISYSQKKG GRAISETYEI

UniProtKB: Small ribosomal subunit protein uS3m

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Macromolecule #3: Ribosomal protein S4

MacromoleculeName: Ribosomal protein S4 / type: protein_or_peptide / ID: 3 / Details: GWHPBJSH000696 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 43.438488 KDa
SequenceString: MWLLKKLIQR DIDMSPLRFQ TCRLLLGNVW NRELTIIQRR ILRRLRNRKR SIKKRKIYSK KYLTSYIQLQ TTRKLSLFYG DLPITEMHR GTKRTSYIPF LLNLETRFDV ILLRLHFLET IPQARQLISH RRVCVNKGMV SITHLKLSHG DIISFQENNA I IRGEEIRR ...String:
MWLLKKLIQR DIDMSPLRFQ TCRLLLGNVW NRELTIIQRR ILRRLRNRKR SIKKRKIYSK KYLTSYIQLQ TTRKLSLFYG DLPITEMHR GTKRTSYIPF LLNLETRFDV ILLRLHFLET IPQARQLISH RRVCVNKGMV SITHLKLSHG DIISFQENNA I IRGEEIRR SFYKEILVEK IIGKLLHQPL RMWRRSKTEW FHLLKTKRGC RLLLKSRFLQ QLRSSMQEED LERTKKFGSE KV CLGSSFA EHKRMKRNLL KSLFLSKRRK EKNLNLPTRT ISPIVYNSSL SLYSNSTYCF ASPHKLTMKR RIKRIELPTH YLE VNYRTP KAVVFYGPNI GHIPHDIRLK DLNLLLWSRN GRGQNI

UniProtKB: Ribosomal protein S4

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Macromolecule #4: S5 DRBM domain-containing protein

MacromoleculeName: S5 DRBM domain-containing protein / type: protein_or_peptide / ID: 4 / Details: GWHPBJSH015007 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 58.296727 KDa
SequenceString: MSFRTRASSW TRLLLRRNPR SSFSRQISSL DASGSAFPGR ITPWISSQTH RFSSATTISP AEADRVVRDL LAEVEKEKQR EREERQRQG LDCKDIDDED EDEEDYLGIE PFIEKLKKQN LKDDGELNRR EESSDSDSEL DEVDWDEERK KEDMFNKKFQ R HKELLQTL ...String:
MSFRTRASSW TRLLLRRNPR SSFSRQISSL DASGSAFPGR ITPWISSQTH RFSSATTISP AEADRVVRDL LAEVEKEKQR EREERQRQG LDCKDIDDED EDEEDYLGIE PFIEKLKKQN LKDDGELNRR EESSDSDSEL DEVDWDEERK KEDMFNKKFQ R HKELLQTL TKSETLDEAY KWMTKLDKFE EKHFKLAPEY RVIGELMNRL KVAEGKDKFI LQQKINRAMR LVEWKEAFDP NN PANYGVI ERDNVQGGEE KEERLVADGS KDDNDDNEEE FDDMKERDDI LLEKLNAIDK KLESKLSELD HTFGKKGKRL EEE IRDLAE ERNALTEKKR QPLYRKGYDV HVIDVKKVAK VTKGGRVERY TALMVCGNYE GVIGYAKAKA ETGQSAMQKA YEKC FQNLH YIERHEEHTI AHAIQTSYKK TKLYLWPAPT TTGMKAGRVV KTMLLLAGFK NIKSKVIGSR NSYNTVKAVL KALNA VETP KDVQEKFGRT VVEKYLL

UniProtKB: S5 DRBM domain-containing protein

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Macromolecule #5: bS6m

MacromoleculeName: bS6m / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 16.011447 KDa
SequenceString:
MPLYDCMLLF KPIIRKEGLI DLVARIGKHV YSKNGVLTEV KSFGKVELGY GIKKLDGRHY QGQLMQITMM ATPNINKELH YLNKDDKLL RWLLVKHRDI KIGSSEMEDR QDDSNRVTNR SLYDESSTDE DDDILGLSR

UniProtKB: Uncharacterized protein

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Macromolecule #6: uS7m,Small ribosomal subunit protein uS7m,Small ribosomal subunit...

MacromoleculeName: uS7m,Small ribosomal subunit protein uS7m,Small ribosomal subunit protein uS7m
type: protein_or_peptide / ID: 6
Details: ...Details: GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 17.955059 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) GGLDGEQKLL IKKLVNFRMK EGKRTRVRAI VYQTFHRPA RTERDVIKLM VDAVENIKPI CEVAKVGVAG TIYDVPGIVA RDRQQTLAIR WILEAAFKRR ISYRISLEKC S FAEILDAY ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) GGLDGEQKLL IKKLVNFRMK EGKRTRVRAI VYQTFHRPA RTERDVIKLM VDAVENIKPI CEVAKVGVAG TIYDVPGIVA RDRQQTLAIR WILEAAFKRR ISYRISLEKC S FAEILDAY QKRGSARRKR ENLHGLASTN RSFAHFRWW

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Macromolecule #7: 40S ribosomal protein S15a

MacromoleculeName: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 7 / Details: GWHPBJSH002412 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 14.874127 KDa
SequenceString:
MGRRILNDAL RTIVNAERRG KASVELKPIS TVMSSFLRIM KEKGYIKNFQ VHDPHRVGRI TVDLQGRVND CKALTYRQDV KAKEIEEYT ERTLPTRQWG YVVVTTPDGI LDHEEAIKRN VGGQVLGFFY

UniProtKB: 40S ribosomal protein S15a

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Macromolecule #8: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 8 / Details: GWHPBJSH080139 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 42.32841 KDa
SequenceString: MLSRLFLRSS NLRLATLVSS KSNSQIFSSF IRPLSTNSSG GGGGNDSGNG GNRNDAPWSF SGVNDGKSDP FSSFESGSPG GDGKWPKEE PKRWNMKEEG DEKAVFGDVS NGFGDVKSSG WDVSSKPWDL KEDEEDGKVV FDTSGEMPVS FDNSLVNEEE E RAKKELFE ...String:
MLSRLFLRSS NLRLATLVSS KSNSQIFSSF IRPLSTNSSG GGGGNDSGNG GNRNDAPWSF SGVNDGKSDP FSSFESGSPG GDGKWPKEE PKRWNMKEEG DEKAVFGDVS NGFGDVKSSG WDVSSKPWDL KEDEEDGKVV FDTSGEMPVS FDNSLVNEEE E RAKKELFE KEEKELTEVI KGPDRAFGDL IAKSGITDEM LDSLIALKDF QGVQGLPPLT EIENLRREKS SKKSSRAEIE LQ MQEEIAK ARVRQVDEAG RAYGTGRRKC SIARVWIVPG KGKFLVNDKE FDVYFPMLDH RAALLRPLAE TKTLGSWDIN CTV TGGGTT GQVGAIQLGI SRALQNWEPD MRTALRAAGF LTRDSRVVER KKPGKAKARK SFQWVKR

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Macromolecule #9: Small ribosomal subunit protein uS10 domain-containing protein

MacromoleculeName: Small ribosomal subunit protein uS10 domain-containing protein
type: protein_or_peptide / ID: 9 / Details: GWHPBJSH050210 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 25.251248 KDa
SequenceString: MIGVLRRSSL PSRHALSAAL TSFDSGLSLN ITSAATGASV KLNLVGNGST LSSASFVISA RDFHIRSEPS MVSPAKISFR GYATGSESS IGDSAKIKIS PENVKPLSKK KKGGGESTSK IKGTRICIAI RSFDNPEKEA WCLPPHSRYA AMPDTRTLYT V LRSPHVDK ...String:
MIGVLRRSSL PSRHALSAAL TSFDSGLSLN ITSAATGASV KLNLVGNGST LSSASFVISA RDFHIRSEPS MVSPAKISFR GYATGSESS IGDSAKIKIS PENVKPLSKK KKGGGESTSK IKGTRICIAI RSFDNPEKEA WCLPPHSRYA AMPDTRTLYT V LRSPHVDK KSREQFEMRF KKRFLVIKAQ SHELSNKLFW LKRYRILGAQ YELQFHCKTR LDMSPVLGSK

UniProtKB: Small ribosomal subunit protein uS10 domain-containing protein

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Macromolecule #10: Ribosomal protein S11

MacromoleculeName: Ribosomal protein S11 / type: protein_or_peptide / ID: 10 / Details: GWHPBJSH051931 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 33.108223 KDa
SequenceString: MNGVSRHLRA SSLPSLIRSY GGIKSVCRFS CQSDGSSGGR FGEQGRVPGD SENKSGLPDP NASPSKSFSE MRSGLLNKIG DGGYSAPNA PPTFRNPLRS RLPNTLPSQS GQMNQEGLPN TGGTGFSAPS YQSFTQSNLL KDNFRTGGIS KDLDFVRGVI E DDEGRRAT ...String:
MNGVSRHLRA SSLPSLIRSY GGIKSVCRFS CQSDGSSGGR FGEQGRVPGD SENKSGLPDP NASPSKSFSE MRSGLLNKIG DGGYSAPNA PPTFRNPLRS RLPNTLPSQS GQMNQEGLPN TGGTGFSAPS YQSFTQSNLL KDNFRTGGIS KDLDFVRGVI E DDEGRRAT GLFSHFHRPN LETNADIIHI KLLRNNTFVT VTDSKGNVKC KATSGSLPDL KGGRKMTSYT ADATAENIGR RV KAMGLKS VVVKVNGFTH FKKKRNAIVA FRSGYSNSRN DQNPIVYIED TTRKAHNGCR LPRKRRV

UniProtKB: Ribosomal protein S11

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Macromolecule #11: Small ribosomal subunit protein uS12m

MacromoleculeName: Small ribosomal subunit protein uS12m / type: protein_or_peptide / ID: 11 / Details: GWHPDUBS019876 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 14.294818 KDa
SequenceString:
MPTFNQLIRH GREEKRRTDR TRALDKCPQK LGACLRVSTR TPKKPNSALR KIAKVRLSNR HDIFAYIPGE GHNLQEHSQV LIRGGRVKD LPGVKFHCIR GVKDLMGIPG RRRGRSKYGA EKPKSI

UniProtKB: Small ribosomal subunit protein uS12m

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Macromolecule #12: uS13m

MacromoleculeName: uS13m / type: protein_or_peptide / ID: 12 / Details: GWHPBJSH064799 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 17.398301 KDa
SequenceString:
MLGLRRSAAT LFDHSQSLLR NLSFHGLRVQ GIRVGNAEVP NHKPLKTGLQ EVYGIGRRKS HQVLCGLGIT NKLARDLTGK ELIDLREEV GMHQHGDELR RRVGSEIQRL VEVDCYRGSR HRHGMPCRGQ RTKTNARTKK GKRVAIAGKK KAPRK

UniProtKB: Uncharacterized protein

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Macromolecule #13: Small ribosomal subunit protein uS14m

MacromoleculeName: Small ribosomal subunit protein uS14m / type: protein_or_peptide / ID: 13 / Details: GWHPDUBS019475 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 17.92885 KDa
SequenceString:
MANLRGVLTN VSSYCQRSFS QSALLWRSAV KPPNNLAQIQ SRQISTSLAK HSGTEQGVKR NSADHRRRLL AARFELRRKL YKAFCKDPE LPSEMREKNR YKLSKLPRNS AFTRIRNRCV FTGRSRSVTE LFRMSRICFR GLANKGELVG IKKSSW

UniProtKB: Small ribosomal subunit protein uS14m

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Macromolecule #14: Small ribosomal subunit protein uS15c

MacromoleculeName: Small ribosomal subunit protein uS15c / type: protein_or_peptide / ID: 14 / Details: GWHPBJSH065138 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 47.211242 KDa
SequenceString: MAFHLARRKQ RLHSNPYLIH LFSTSSSSAS PQDGNESGEQ QPSQSPSDFK ISSYFSGIKS SLKQQPQHQA QDGRRQLAGF DPKAPSFSG SGGDFQDVRR NLNEFRRRAA APPARDLQDL YKQNVLSKSG DLGAGKVEGS PFENLKKNLR QMRPQETRWS N LSSLQSIM ...String:
MAFHLARRKQ RLHSNPYLIH LFSTSSSSAS PQDGNESGEQ QPSQSPSDFK ISSYFSGIKS SLKQQPQHQA QDGRRQLAGF DPKAPSFSG SGGDFQDVRR NLNEFRRRAA APPARDLQDL YKQNVLSKSG DLGAGKVEGS PFENLKKNLR QMRPQETRWS N LSSLQSIM KAKDSGNVRS NVFGGGEGLP ISVFGEEIEE RKKMGDDSEE MKSEFIKSYN TKELGEILRQ YRPEGKREEG WF SLQELNQ RLVKLREVEE AAAQGARKGI AFDDLRSEIQ QARKSQAFQN LDFFSVLNGT PKYLLEPPKD ELVQTYFHPD NMS SAEKMK IELAKVREEF KMSESDCGSA RVQVAQLTTK IKHLSSVLHK KDKHSKKGLI AMVHRRKKLL KYMRRTDWDS YCLS LSKLG LRDNPDYKF

UniProtKB: Small ribosomal subunit protein uS15c

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Macromolecule #15: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 15 / Details: GWHPBJSH018119 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 15.330757 KDa
SequenceString:
MVVRLRLSRL GCKNRPFFRV MATDSRSPRD GKHIEVLGYF NPLPGQDGGK RMGLKFDRIK YWLSVGAQAS DPVQRLLFRS GLLPPPPMV AMGRKGGERD TRPVDPMTGR FVDAEKKTPV IIDNQPKEED NAQDKSP

UniProtKB: 30S ribosomal protein S16

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Macromolecule #16: Small ribosomal subunit protein uS17c

MacromoleculeName: Small ribosomal subunit protein uS17c / type: protein_or_peptide / ID: 16 / Details: GWHPBJSH049400 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 12.090024 KDa
SequenceString:
MKPVIGTVVS NKMQKSVVVA VDRLFHNKLY NRYVKRTSKF MAHDETNACN IGDRVKLDPS RPLSKHKNWI VAEIIKKARI YSPQAAAAA ALSFSAAKSP SESSVPPVSS S

UniProtKB: Small ribosomal subunit protein uS17c

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Macromolecule #17: Small ribosomal subunit protein bS18c

MacromoleculeName: Small ribosomal subunit protein bS18c / type: protein_or_peptide / ID: 17 / Details: GWHPBJSH076274 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 27.106117 KDa
SequenceString: MKLAHSAIRS LSDVASFQTC RPFVSRSLST NANQDDWMFG GKDEKANSFF QHLGKAEKDK RDYTGFSRSQ GNGSRFDPSS DGVDGKLKE AALIYNVDDD EGVKDGYSFR PDVNSWGVNQ FPRDMNFRRQ MQRPRQNNKA EITTEEVLKK ADFRNVRFLA Q FITEAGIL ...String:
MKLAHSAIRS LSDVASFQTC RPFVSRSLST NANQDDWMFG GKDEKANSFF QHLGKAEKDK RDYTGFSRSQ GNGSRFDPSS DGVDGKLKE AALIYNVDDD EGVKDGYSFR PDVNSWGVNQ FPRDMNFRRQ MQRPRQNNKA EITTEEVLKK ADFRNVRFLA Q FITEAGIL VKRKQTGISA KAQRKIAREI KTARAFGLMP FTTMGTKAFT FGKTMENRDQ DFEYEVIDDD DEYDDNTPE

UniProtKB: Small ribosomal subunit protein bS18c

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Macromolecule #18: Small ribosomal subunit protein uS19m

MacromoleculeName: Small ribosomal subunit protein uS19m / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 23.675223 KDa
SequenceString: MAFCTKLGGH WKQGVNVPVS SMLGSLRYMS TKLYIGGLSP GTDEHSLKDA FSSFNGVTEA RVMTNKVTGR SRGYGFVNFI SEDSANSAI SAMNGQELNG FNISVNVAKD WPSLPLSLDE SIEEAEKKEN KMMSRSVWKD PFVDAFLMKK KNAALNRKIW S RRSTILPE ...String:
MAFCTKLGGH WKQGVNVPVS SMLGSLRYMS TKLYIGGLSP GTDEHSLKDA FSSFNGVTEA RVMTNKVTGR SRGYGFVNFI SEDSANSAI SAMNGQELNG FNISVNVAKD WPSLPLSLDE SIEEAEKKEN KMMSRSVWKD PFVDAFLMKK KNAALNRKIW S RRSTILPE YVDSAVRIYN GKTHVRCKIT EGKVGHKFGE FAFTRKVKKH AKAK

UniProtKB: Small ribosomal subunit protein uS19m

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Macromolecule #19: Ribosomal protein S21

MacromoleculeName: Ribosomal protein S21 / type: protein_or_peptide / ID: 19 / Details: GWHPBJSH012122 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 11.541774 KDa
SequenceString:
MNTIAKRVTG LLTRPSHSQL QQERGIRVKV FSGDLDKALT ILQRKMQSSG MERLIKAQQT HHIKNSEKKV LARKNLERKI KSIDFARKL QSILIKKVRG L

UniProtKB: Ribosomal protein S21

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Macromolecule #20: 30S ribosomal protein S31, mitochondrial

MacromoleculeName: 30S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 20 / Details: GWHPDUBS022287 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 10.524609 KDa
SequenceString:
MMAAAQWCSS MTRLVMMMQR TSPALACSAR YSSLCPAAAS MEVCGRGDSK TKKGKRFKGS YGNSRGKKQK MIERIKDKLE LPRSTPWPL PFKLI

UniProtKB: 30S ribosomal protein S31, mitochondrial

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Macromolecule #21: Small ribosomal subunit protein mS23

MacromoleculeName: Small ribosomal subunit protein mS23 / type: protein_or_peptide / ID: 21 / Details: GWHPBJSH071753 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 21.934494 KDa
SequenceString: MSWMKGDLLS KTRRLVGGLA TREPVWLKAM EASPPPVFPR SNGNLKKIVL PEDPYVRRFA RKHPEAKLVE PNKVSAFIPD QARVYGCRV LELTKNGISE DDAMSVANME YLAERKEKKK AYKRLKELAV LQDKAPPPKP YLSTKKEMQT QEKKPPTDPP S VRRLVNQL ...String:
MSWMKGDLLS KTRRLVGGLA TREPVWLKAM EASPPPVFPR SNGNLKKIVL PEDPYVRRFA RKHPEAKLVE PNKVSAFIPD QARVYGCRV LELTKNGISE DDAMSVANME YLAERKEKKK AYKRLKELAV LQDKAPPPKP YLSTKKEMQT QEKKPPTDPP S VRRLVNQL KQQKDVLLQD KPGGSANQDH WIDE

UniProtKB: Small ribosomal subunit protein mS23

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Macromolecule #22: mS26

MacromoleculeName: mS26 / type: protein_or_peptide / ID: 22 / Details: GWHPBJSH068774 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 23.093625 KDa
SequenceString: MSLCNQLSRL VKQSPLTKQS LRSFSAEAAT HHRHHQETHS FLEPESYIGS WEAPKDPKDA ERMLAQLRRD YAKKVSLYRK DYVHEIEML RVEKQRKDEA RLVAERAANE ERRRLKAEAA KVRAEERKIF QDEFRKTLMK ERAEKLEFWR MTGLKREEKK K ARKKLLHE ...String:
MSLCNQLSRL VKQSPLTKQS LRSFSAEAAT HHRHHQETHS FLEPESYIGS WEAPKDPKDA ERMLAQLRRD YAKKVSLYRK DYVHEIEML RVEKQRKDEA RLVAERAANE ERRRLKAEAA KVRAEERKIF QDEFRKTLMK ERAEKLEFWR MTGLKREEKK K ARKKLLHE QSSLWIEPKE LEKKITEALV DSTTL

UniProtKB: Uncharacterized protein

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Macromolecule #23: Small ribosomal subunit protein mS29

MacromoleculeName: Small ribosomal subunit protein mS29 / type: protein_or_peptide / ID: 23 / Details: GWHPBJSH054439 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 54.288594 KDa
SequenceString: MFRSVVRITS RNFNLNRRSL IPNDAVSHFP SPPSASAATL IPSLDHAHRR RFSSSKSTKS SITKTKKSEG KKSKSKGGES GAAAGAEDG EFGGSAGDDL EAGRAKRLAD DEKTPSLDVG PNGRPLFTPR DVTLSKLSQK DIGSYFNFDE AALKAVLPEG L ASGIEDEF ...String:
MFRSVVRITS RNFNLNRRSL IPNDAVSHFP SPPSASAATL IPSLDHAHRR RFSSSKSTKS SITKTKKSEG KKSKSKGGES GAAAGAEDG EFGGSAGDDL EAGRAKRLAD DEKTPSLDVG PNGRPLFTPR DVTLSKLSQK DIGSYFNFDE AALKAVLPEG L ASGIEDEF KESWRPALLV RKSFLDLRDN FRRIADPPMW PSEGKGGVKP KKQIILDGPV KSGKSIALAM LVHWARDEGW LV LYAPKGR DWTHGGYFYK NQHTGFWDTP LQAESILKDF VKFNEPRLRE LRCNVYDPIV LGEGAGVGYL KGQETMPIPE DST LYDLVQ MGINSTHAAV SVVVRLRKEL SLVKDVPVLI AIDQYNNWFT FSEFEEPVTP RSCRPIHARE LTTVNAFRSM MHDD MMVGA FSHSTAVGKL RKDLPDVPAD ARQNFPRYSL DEAEAVCYYY LRQRLVRREV FSEENWKKIY YLANGNGAEM RWLVP FMR

UniProtKB: Small ribosomal subunit protein mS29

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Macromolecule #24: BnaC06g06470D protein

MacromoleculeName: BnaC06g06470D protein / type: protein_or_peptide / ID: 24 / Details: GWHPBJSH057126 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 53.476242 KDa
SequenceString: MRGAIGRRLS NPSNGFSLAS IVKHTPFLVQ PTSHFSSSSS DGGAVRGRGR GRGGSGSPAA GAGQFGFNRE PEKANEPVGQ ARGSSESQS PGGYGHGRGR PIQSDPISPP FSSFVKPDSH SVGRGRGSLR SDPVSPFSPE PPRHPAPPQP QQPRFEPAKD G VQGSPPFA ...String:
MRGAIGRRLS NPSNGFSLAS IVKHTPFLVQ PTSHFSSSSS DGGAVRGRGR GRGGSGSPAA GAGQFGFNRE PEKANEPVGQ ARGSSESQS PGGYGHGRGR PIQSDPISPP FSSFVKPDSH SVGRGRGSLR SDPVSPFSPE PPRHPAPPQP QQPRFEPAKD G VQGSPPFA KLEATKDATS PPPPPPPGAP NNISNALGSG AGRGKPFVQN EDNRHIQRSP QPLQRQKRVQ PPKDTTPRPQ LS PEEAGRR ARSQLSRGEA EGGSGGGVRG RGGGRGRGRG ARGRGRGRGG EGWRDDKKEE EAEQEALSVF VGDNADGEKF AKK MGDEIM AKLAEGYEDI CERALPSTAH DALVDAYDTN LMIECEPEYL MPDFGSNPDI DEKPPMPLRE CLEKVKPFIV ALEG IKDQE EWEEAIGEAM AEAPRMKEIV DHYSGPDRVT AKKQNEELDR IATTVPQSAP DSVKRFADRV ALSLKSNPGW GFDKK YQFM DKLVLEVSQN YK

UniProtKB: BnaC06g06470D protein

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Macromolecule #25: MOSC domain-containing protein

MacromoleculeName: MOSC domain-containing protein / type: protein_or_peptide / ID: 25 / Details: GWHPDUBS036572 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 11.631559 KDa
SequenceString:
MASGSLKNLV TSAVTVGVTE ARARIFGHML NPTGQRSPHK ILRKKLFGDK VAEWYPYDIK NEDPNVLARE EKERLSKLEM LKRRNKGPP QKGHGRRAAK RNK

UniProtKB: MOSC domain-containing protein

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Macromolecule #26: Mitochondrial 28S ribosomal protein S34

MacromoleculeName: Mitochondrial 28S ribosomal protein S34 / type: protein_or_peptide / ID: 26 / Details: GWHPBJSH018393 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 16.650186 KDa
SequenceString:
MAATLMNRAI SRTETVGAFR LSLNLLRNFS APAAAAAASP STENPSSDSI KPKRRKKKNL IEVAQFLPNW GIGYHMAKAH WNGVSYEIT KINLYKDGRH GKAWGIVHKD GLRAAEAPKK ISGVHKRCWK YIPNLSKTTP ATTGSAPAAE VQAA

UniProtKB: Mitochondrial 28S ribosomal protein S34

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Macromolecule #27: EngC GTPase domain-containing protein

MacromoleculeName: EngC GTPase domain-containing protein / type: protein_or_peptide / ID: 27 / Details: GWHPBJSH063220 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 55.785578 KDa
SequenceString: MQISSLSIIR HSSPFLLRRA SIHHGGCGVR LGTGIGIRRS FHLLTLSARR DNPDVSRKPH PSKNMLRAKH IGKDHSSLAP VLSPDHNPS LLPSQAIGTV ATAQANFMRV VVQDGDGDGD DRSSSTSSSK DGVELLCVVR AVLKKIRRRV LVGDKVLVGS I DWVDRRGM ...String:
MQISSLSIIR HSSPFLLRRA SIHHGGCGVR LGTGIGIRRS FHLLTLSARR DNPDVSRKPH PSKNMLRAKH IGKDHSSLAP VLSPDHNPS LLPSQAIGTV ATAQANFMRV VVQDGDGDGD DRSSSTSSSK DGVELLCVVR AVLKKIRRRV LVGDKVLVGS I DWVDRRGM IENVFQRRSE ILDPPVANVD HLLVLFSLDQ PKLEPFTLTR FLVEAESTGI PLTLALNKCE LITEEELESW KM RLRGWNY EPFFCSVGTK EGLDAIAFVL RNQTSVIVGP SGVGKSSLIN ILRSSSGGGD VEDENWFEPI IGNKWFEDQR VGE VSTRSG RGKHTTRNVS LLPITEGGYL ADTPGFNQPS LLKVTKHSLA LCFPEIRKMI EEEKCGFKDC LHIGEPGCVV KGEW ERYPY YLQLLDEIRV REEFQLRTFG TKREGDVRYK VGGMGVKQAE PRLMPKKHRR ESRKKVKQTM ISELDEYEDE ESDLD IEDD PIVQAIENEK ER

UniProtKB: EngC GTPase domain-containing protein

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Macromolecule #28: Small ribosomal subunit protein mS35 mitochondrial conserved doma...

MacromoleculeName: Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein
type: protein_or_peptide / ID: 28 / Details: GWHPBJSH049245 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 48.589312 KDa
SequenceString: MRGALFRNAS LCARRLLLSP RINTHQPSSS SNARFVAPIA TPFPPRLFSS ESDSSGENPP PPPESSSPIE SSNKKDLAVE DVGNKELKA RIEKYFNEGN EDALPGIIEA LLQRRLADKH ADTDDEVMDS LQNQPFKDDV KDEDFESDFE EAHSTDDELE D LYNSPEYV ...String:
MRGALFRNAS LCARRLLLSP RINTHQPSSS SNARFVAPIA TPFPPRLFSS ESDSSGENPP PPPESSSPIE SSNKKDLAVE DVGNKELKA RIEKYFNEGN EDALPGIIEA LLQRRLADKH ADTDDEVMDS LQNQPFKDDV KDEDFESDFE EAHSTDDELE D LYNSPEYV KKKMQNNEFF NMDEKKWDVI VRDGIRHGIL KDTKECEEIL EDMLHWDKLL PDDLKKKVEA KFNELGDMCE RG EIEPEAA YELFKEFEDE MVIQYGDQME AAGPPKFDET DPSYSNTNLD DPPGKGPILR WQSRIVFAPG GDAWHPKNRK VKL SVTVKE LGLSKHQARR LRELVGKRYD SGKDELTITS ERFEHREENR KDCLRTLYGL IEEAAKANKI AEDIRTAYVK QRLQ ANPAF MQKLQAKIMR SKESNPINA

UniProtKB: Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein

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Macromolecule #29: IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-cont...

MacromoleculeName: IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein
type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 9.227926 KDa
SequenceString:
MGRKAGNLYI NPKKLGNLAK PCMKEMVSFL NCMALNNIKD DKCEKQKQLL SVCMQGQSDH KNKSWGNINY HLQRLTRGRK

UniProtKB: IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein

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Macromolecule #30: Small ribosomal subunit protein mS38

MacromoleculeName: Small ribosomal subunit protein mS38 / type: protein_or_peptide / ID: 30 / Details: GWHPDUBS042575 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 14.562171 KDa
SequenceString:
MANLMQRFIR NQSCLRAIPK LTPNLIPHQK PCIIINNNVE SPFPLTNPVI AFSGSGIKPD EALRFYPSFP IGYGLNPSVV HGSGLTDTV VEEKEKEVVI HADSVKKKRK KKMNKHKYRK LRKKQGRKS

UniProtKB: Small ribosomal subunit protein mS38

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Macromolecule #31: IGR domain-containing protein

MacromoleculeName: IGR domain-containing protein / type: protein_or_peptide / ID: 31 / Details: GWHPBJSH068514 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 12.49895 KDa
SequenceString:
MAWMQMIQSA RFLRKTQPFS TPSLARFYSK PAPYAVKVGI PEFLSGIGGG VETHVARLET ELGDLSKLLV TRTLKLKKFG IPCKHRKLI LKYSQKYRLG LWKPRADAIK A

UniProtKB: Small ribosomal subunit protein mS41 SAM domain-containing protein

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Macromolecule #32: mS45

MacromoleculeName: mS45 / type: protein_or_peptide / ID: 32 / Details: GWHPBJSH025481 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 44.013531 KDa
SequenceString: MFKLSRMIPR LQSVSLNPST TASGSLIENA PRIFQSLGNR AFSGKSSSDG SGSNENGWDI ATGGSFGGGT TGGSDDLDWD HKSMWSTGL SKEHFDGVSV GRQNPSSENA VSDSGDVMSK LGPREVAMVN EMNEYDDMIK EIEKDNRHSR VFVDGIKQKM M EMSVLLKQ ...String:
MFKLSRMIPR LQSVSLNPST TASGSLIENA PRIFQSLGNR AFSGKSSSDG SGSNENGWDI ATGGSFGGGT TGGSDDLDWD HKSMWSTGL SKEHFDGVSV GRQNPSSENA VSDSGDVMSK LGPREVAMVN EMNEYDDMIK EIEKDNRHSR VFVDGIKQKM M EMSVLLKQ VKEPGARGSY LKDSEKTEMY RLHKENPEVY TVERLAKDYR IMRQRVHAIL FLKEDEEEEE RKLGRPLDDS VE RLLDEYP EFFVSHDREF HVASLSYKPD FKVMPEGWDG TIKDMDEVHY EISKKEDDIL YEEFLRRFEF NKLKWKGEVK CHK YSRRRS SDGWKITVEK LGPQGKRGNG GGWKFVSLPD GSSRPLNEVE KMYVKREAPR RRRKILP

UniProtKB: Uncharacterized protein

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Macromolecule #33: mS47

MacromoleculeName: mS47 / type: protein_or_peptide / ID: 33 / Details: GWHPBJSH021410 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 45.518668 KDa
SequenceString: MQTVRALRRV TKPSQWVRSV SQGKRSFSAL PNFSASDADV QDQVSVEGKA KSRAAILDRP SSLNALSAPM VGRLKRLYES WEENPAISF VLMKGSGKTF CSGADVLPLY HSINEGNTEE CKHFFGSLYN FVYLQGTYLK PNIAIMDGVT MGCGGGISIP G MFRVATDK ...String:
MQTVRALRRV TKPSQWVRSV SQGKRSFSAL PNFSASDADV QDQVSVEGKA KSRAAILDRP SSLNALSAPM VGRLKRLYES WEENPAISF VLMKGSGKTF CSGADVLPLY HSINEGNTEE CKHFFGSLYN FVYLQGTYLK PNIAIMDGVT MGCGGGISIP G MFRVATDK TVLAHPEVQI GFHPDAGASY YLSRLPGYLG EYLALTGQKL DGVEMIACGL ATHFCLHSRL GMVEERIGKL LT DDPTVIE ASLAQYSDLV YPDNTSVLHK IEMIDRYFGL DTVEEIIEAM ENEVADSGNE WCKKTLKQVK EASPLSLKIT LQS IREGRF QTLDQCLTRE YRISLCGVSK TVSGDFCEGI RARLVDKDFA PKWDPPRLED VSKDMVDCYF SPATDADDSE SELK LPTAQ REPYF

UniProtKB: 3-hydroxyisobutyryl-CoA hydrolase

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Macromolecule #34: (rape) hypothetical protein

MacromoleculeName: (rape) hypothetical protein / type: protein_or_peptide / ID: 34 / Details: GWHPBJSH050066 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 43.364805 KDa
SequenceString: MQYLSNSRRI ARALKPLALL EADVRFASHV VDSTQWQNHR SPSVSVSPGM NTQSHMTGLM MLRSSFSSEA KLVEDPTEAV KELHGKILD SVNVKRSMPP NALLWSLIEN CRKEDDISFL FDALQNLRRF RLSNLRIHDN FNCNLCRQVA KTCVRVGAIN H GKRALWKH ...String:
MQYLSNSRRI ARALKPLALL EADVRFASHV VDSTQWQNHR SPSVSVSPGM NTQSHMTGLM MLRSSFSSEA KLVEDPTEAV KELHGKILD SVNVKRSMPP NALLWSLIEN CRKEDDISFL FDALQNLRRF RLSNLRIHDN FNCNLCRQVA KTCVRVGAIN H GKRALWKH NVHGLTPSVA SAHHLLSYAL EHKNSNLMDE VMKLLKANDL PLQPGTADLV FRICHETDSW DLLAKYSKKF CK AGVKLRK TTFDVWMEFA AKRGDTESLW KVDKLRSETY TQHTLSAAFS CAKGFLLEHK PEEAAAVIQI ICQAYPDEKK SAL EAEFKK LVNEWPVDVL KHQNEEDKKA VAASLKSDIP AMVNALVNSG LRVSVDLDEL NKNEALLS

UniProtKB: (rape) hypothetical protein

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Macromolecule #35: PROP1-like PPR domain-containing protein

MacromoleculeName: PROP1-like PPR domain-containing protein / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 82.64882 KDa
SequenceString: MKSFLLSRQA IHRISLLSSK TPTFCRNFSA ITSTISHSDR HLRSYDEQTP FQNVEIPRPI SSFNRYFHFT RESRLSESSA AIDDSNDQE EDDEDGTTNE FLSRFVWIMR GKVSEAYPDC DKKMIDGMLL LIVEKVVEEI ERGGFNKVGS APPSPSSEFS D DLWATIWE ...String:
MKSFLLSRQA IHRISLLSSK TPTFCRNFSA ITSTISHSDR HLRSYDEQTP FQNVEIPRPI SSFNRYFHFT RESRLSESSA AIDDSNDQE EDDEDGTTNE FLSRFVWIMR GKVSEAYPDC DKKMIDGMLL LIVEKVVEEI ERGGFNKVGS APPSPSSEFS D DLWATIWE VSNTVLKDME KERKKEKMKQ YVQSPEVMEM CRFAGEIGIR GDLLRELRFK WAREKMDDAE FYESLEQQRD LD NSIRESE TVDGEVEEEG FVPSDEVESR SISLPKRKGK LKYKIYGLEL SDPKWVEMAD KIHEAEEEAD WREPKPVTGK CKL VMEKLE SLQEGDDPSG LLAEWAELLE PNRVDWIALI NQLREGNTHA YLKVAEGVLD EKSFNASISD YSKLIHIHAK ENHI EDVER ILKKMSQNGI FPDILTATAL VHMYSKSGNF ERATEAFENL KSYGLRPDEK IYEAMILGYV NAGKPKLGER LMKEM QAKE LKASEEVYMA LLRAYAQMGD ANGAAGISSS MQYASDGPLS FEAYSLFVEA YGKAGQVDKA KSNFDEMRKL GHKPDD KCI ANLVRAYKGE NSLDKALRLL LQLEKDGIEI GVITYTVLVD WMANLGLIEE AEQLLVKISQ LGEAPPFELQ VSLCCMY SG VRNEKKTLQA LGVLEAKRDQ MGPNEFDKVI SALKRGGFEK DARRMYKYME ARKFLPSQRL QMDMVAPPRA FGSGSGRV R RFNS

UniProtKB: PROP1-like PPR domain-containing protein

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Macromolecule #36: RRM domain-containing protein

MacromoleculeName: RRM domain-containing protein / type: protein_or_peptide / ID: 36 / Details: GWHPBJSH080930 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 16.001051 KDa
SequenceString:
MHYMGLFSRA GNIFKQPRAM QAANAMLQGN LSLTPSKLFV GGLSPTTDVD ILKEVFGKFG KIVDVVVVSD RETGVSKGFG FVTYDSIDA ANKAMQQMND QELDGRIIGV NPADSGGGGG GFARRGGYGG GRGGFGRGGF GRGGFGRGGY NFVR

UniProtKB: RRM domain-containing protein

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Macromolecule #37: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 37 / Number of copies: 1
Source (natural)Organism: Brassica oleracea var. botrytis (cauliflower)
Molecular weightTheoretical: 504.947344 KDa
SequenceString: CAUAGUCAAA AGAAGAGUUU GAUUCUGGCU CAGAAGGAAC GCUAGCUAUA UGCUUAACAC AUGCAAGUCG AACGUUGUUC UAAAAAAAA AAUUUUUUUU AAGAGUUGAG AACAAAGUGG CGAACGGGUG AGUAAGGCGU GGGAAUCUGC CGAACAGUUC G GGCUAAAU ...String:
CAUAGUCAAA AGAAGAGUUU GAUUCUGGCU CAGAAGGAAC GCUAGCUAUA UGCUUAACAC AUGCAAGUCG AACGUUGUUC UAAAAAAAA AAUUUUUUUU AAGAGUUGAG AACAAAGUGG CGAACGGGUG AGUAAGGCGU GGGAAUCUGC CGAACAGUUC G GGCUAAAU CCCGAAGAAA GCUAGAAAGC GCUGUUUGAU GAGCCUGCGU AGUAUUAGGU AGUUGGUUAG GUAAAGGCUG AC CAAGCCG AUGAUGCUUA GCUGGUCUUU UCGGACGAUC AGCCACACCG GGACUGAGAC ACGGCCCGGA CUCCCACGGG GGG CAGCAG UGGGGAAUCU UGGACAAUGG GCGCAAGCCU GAUCCAGCAA UAUUGCGUGA GUGAAGAAGG GCAAUUCCGC UCGU AAAGC UCUUUCGUCG AGUGCGCGAU CAUGACAUGA CUCGAGGAAG AAGCCCCGGC UAACUCCGUG CCAGCAGCC(G7M) C GGUAAAAC GGGGGGGGCA AGUGUUCUUC GGAAUGACUA GGCGUAAAGG GCACGUAGGC GGUUCAUCGG GUUGAAAGUG AA AGUCGUC AAAAAGUGGC GGAAUGCUUU CGAAACCACU GAACUUGAGU GAGACAGAGG AGAGUGGAAU UUCGUGUGGA GGG GUGAAA UCUACGGAUC UACGAAGGAA CGCCAAAAGC GAAGGCAGCU CUCUGGGUCU CUACCGACGC UGGUGUGCGA AAGC AUGGG GAGCGAACGG GAUUAGAUAC CCUGGUAGUC CAUGCCGUAA ACGAUGAGUG UUCGCCCUUG GUCUAUUCUG AUCUG GGGC UCAGCUAACG CGUUAAACAC UCCGCCUGGG GAGUACGGUC GCAAGACCAA AACUCAAAGG AAUUGACGGG GGCCUG CAC AAGCGGUGGA GCAUGUGGUU UAAUUCGAUA CAACGCGCAG AACCUUACCA GCCCUUGACA UAUGAACGAG AAACCCU AU CCUUAACGGG AUGGUACUGA CUUUCAUACA GGUGCUGCAU GGCUGUCGUC AGCUCGUGUC GUGAGAUGUU UGGUUAAG U CCUAUAACGA GCGAAACCCU CGCCUUGUGU UGCUCAGACA UGCGCCUAAG GAGAACUCCG AAACAAACAA AAAGGUGCG UGACGCACUC ACGAGGGACU GCCAGUGAUA UACUGGAGGA AGGUGGGGAU GACGUCAAGU CCGCAUGGCC CUUAUGGGCU GGGCCACAC ACGUGCUACA AUGGCAAUUA CAAUGGGAAG CAAGGCUGUA AGGCGGAGCG AAUCCGGAAA GAUUGCCUCA G UUCGGAUU GUUCUCUGCA ACUCGGGAAC AUGAAGUUGG AAUCGCUAGU AAUCGCGGAU CAGCAUGCCG CGGUGAAUAU GU ACCCGGG CCCUGUACAC ACCG(4OC)CCGUC ACACCCUGGG AAUUGGUUUC GCCCGAAGCA UCGGACCAAU GAUCACCCA GCACUUCUGU GUAACACUAG UCACACAAAG ACUUUUGGUG GUCUUAUUGA CGCAUACCAC GGUGGGGUCU UCGACUGGGG UGAAGUCG(UR3) AACAAGGUAG CCGUAGG(2MG)G(MA6) (MA6)CCUGUGGCU GGAUUAUCC

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Macromolecule #38: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 38 / Number of copies: 10 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #39: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 39 / Number of copies: 85 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #40: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 40 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #41: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 41 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #42: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 42 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration3 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 50.96 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 165000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 931475
CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.27 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 208855
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final 3D classificationSoftware - Name: cryoSPARC
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL
Output model

PDB-9evt:
Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA

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