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- EMDB-49998: Cryo-EM structure of Methanosarcina acetivorans 70S ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-49998
TitleCryo-EM structure of Methanosarcina acetivorans 70S ribosome
Map data
Sample
  • Complex: Methanosarcina acetivorans 70S ribosome
    • RNA: x 3 types
    • Protein or peptide: x 55 types
  • Ligand: x 2 types
KeywordsArchaea / Methanosarcina acetivorans / 70S ribosome / RIBOSOME
Function / homology
Function and homology information


ribonuclease P activity / tRNA 5'-leader removal / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit ...ribonuclease P activity / tRNA 5'-leader removal / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / cytoplasm / cytosol
Similarity search - Function
Small zinc finger protein HVO_2753-like, zinc-binding pocket / Small zinc finger protein HVO_2753-like / Small zinc finger protein HVO_2753-like, Zn-binding pocket / Ribosomal protein L40e, archaeal / Ribosomal protein L19e, domain 2 / Ribosomal protein L19e, domain 3 / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal / Ribosomal protein S6e, archaeal ...Small zinc finger protein HVO_2753-like, zinc-binding pocket / Small zinc finger protein HVO_2753-like / Small zinc finger protein HVO_2753-like, Zn-binding pocket / Ribosomal protein L40e, archaeal / Ribosomal protein L19e, domain 2 / Ribosomal protein L19e, domain 3 / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal / Ribosomal protein S6e, archaeal / Ribosomal protein S4, archaeal / Ribosomal protein S12, archaea / Ribosomal protein S3, archaeal / 30S ribosomal protein S3Ae / : / Ribosomal protein S7, archaeal / Ribosomal protein S19e, archaeal / Ribosomal protein S11, archaeal / Ribosomal protein S2, archaeal / Ribosomal protein S14, type Z, archaeal / Ribosomal protein S8e, archaeal / Ribosomal protein L15e, archaeal / Ribosomal protein L19e, archaeal / Ribosomal protein L30, archaeal / Ribosomal protein L6P, archaea / Ribosomal protein L14P, archaeal / Ribosomal protein L21e, archaeal / Ribosomal protein L18e, archaea / Ribosomal protein L10e, archaea / Ribosomal protein L3, archaeal / Ribosomal protein L4, archaea / Ribosomal protein L5, archaeal / Ribosomal protein L32e, archaeal / : / Ribosomal protein L7Ae, archaea / Ribosomal protein L30e / Ribosomal L15/L27a, N-terminal / Ribosomal protein L23 / : / Ribosomal protein L2, archaeal-type / metallochaperone-like domain / TRASH domain / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / : / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal L40e family / Ribosomal protein L18e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein 60S L18 and 50S L18e / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L30e signature 2. / Ribosomal protein L39e, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein S27e signature. / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / 60S ribosomal protein L19 / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L30/YlxQ / Ribosomal protein L31e, conserved site / Ribosomal protein L31e signature. / Ribosomal protein L18e / Ribosomal protein L19e, C-terminal domain / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal S24e conserved site / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein S24e signature. / Ribosomal protein L37ae
Similarity search - Domain/homology
DUF1610 domain-containing protein / Large ribosomal subunit protein eL43 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL19 / Small ribosomal subunit protein eS19 / Large ribosomal subunit protein eL42 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein eS17 ...DUF1610 domain-containing protein / Large ribosomal subunit protein eL43 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL19 / Small ribosomal subunit protein eS19 / Large ribosomal subunit protein eL42 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL40 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein eS6 / Large ribosomal subunit protein eL24 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein eL18 / Large ribosomal subunit protein uL16
Similarity search - Component
Biological speciesMethanosarcina acetivorans (archaea)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.92 Å
AuthorsGhosh A / Fordjour GNR / Armache J-P / Ferry JG / Murakami KS / Bevilacqua PC
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35-GM156623 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35-GM127064 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32GM149417 United States
CitationJournal: J Biol Chem / Year: 2025
Title: Cryo-EM study and in vivo chemical mapping of the Methanosarcina acetivorans ribosome and its dimerization via a repurposed enzyme and translation factor.
Authors: George N R Fordjour / Anwesha Ghosh / James G Ferry / Jean-Paul Armache / Philip C Bevilacqua / Katsuhiko S Murakami /
Abstract: Despite the overall conservation of ribosomes across all domains of life, differences in their 3D architecture, rRNA sequences, ribosomal protein composition, and translation factor requirements ...Despite the overall conservation of ribosomes across all domains of life, differences in their 3D architecture, rRNA sequences, ribosomal protein composition, and translation factor requirements reflect lineage-specific adaptations to environmental niches. In the domain Archaea, structural studies have primarily focused on nonmethanogenic thermophiles and halophiles, leaving it unclear whether these represent the broader Archaea domain. Here, we report the cryo-electron microscopy (cryo-EM) structure of the ribosome from Methanosarcina acetivorans, a previously unreported high-resolution structure from a model mesophilic methanogenic archaeon. Compared to ribosomes from extremophiles, the M. acetivorans ribosome has a simplified architecture, lacking paralogous duplications and containing a reduced complement of ribosomal proteins. Structures of the large subunit (50S) from cells grown with either methanol or acetate show conserved rRNA folding and protein composition. High-resolution structures of the 50S subunit from the two growth substrates enabled us to investigate structural properties that may influence in vivo dimethyl sulfate reactivity, an orthogonal chemical approach used to probe RNA structure. We observed good agreement between in vivo dimethyl sulfate reactivity and ribosome structure. Finally, we identify a previously uncharacterized ribosome dimerization mode involving only 50S subunits and mediated by a heterotetrameric complex of PurH and aEF2-proteins with alternative metabolic and translational roles. This macromolecular assembly, which we term the methanogen ribosome dimerization factor, likely mediates ribosome hibernation, revealing an alternative regulatory mechanism in translation.
History
DepositionApr 3, 2025-
Header (metadata) releaseSep 24, 2025-
Map releaseSep 24, 2025-
UpdateNov 5, 2025-
Current statusNov 5, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_49998.png

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Map

FileDownload / File: emd_49998.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.73 Å/pix.
x 480 pix.
= 351.84 Å		0.73 Å/pix.
x 480 pix.
= 351.84 Å		0.73 Å/pix.
x 480 pix.
= 351.84 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.733 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.046
Minimum - Maximum-0.1509495 - 0.25633064
Average (Standard dev.)0.0038819874 (±0.012073599)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 351.84 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_49998_half_map_1.map
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Half map: #1

Fileemd_49998_half_map_2.map
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Sample components

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Entire : Methanosarcina acetivorans 70S ribosome

EntireName: Methanosarcina acetivorans 70S ribosome
Components
  • Complex: Methanosarcina acetivorans 70S ribosome
    • RNA: 16S rRNA
    • Protein or peptide: Small ribosomal subunit protein uS2
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: Small ribosomal subunit protein eS1
    • Protein or peptide: Small ribosomal subunit protein uS4
    • Protein or peptide: Small ribosomal subunit protein eS4
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Small ribosomal subunit protein uS7
    • Protein or peptide: Small ribosomal subunit protein uS8
    • Protein or peptide: Small ribosomal subunit protein eS8
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: Small ribosomal subunit protein uS14
    • Protein or peptide: Small ribosomal subunit protein uS15
    • Protein or peptide: Small ribosomal subunit protein uS17A
    • Protein or peptide: Small ribosomal subunit protein uS19
    • Protein or peptide: Small ribosomal subunit protein eS19
    • Protein or peptide: Small ribosomal subunit protein eS24
    • Protein or peptide: Small ribosomal subunit protein eS27
    • Protein or peptide: Small ribosomal subunit protein eS28
    • Protein or peptide: Small ribosomal subunit protein eS6
    • Protein or peptide: Small ribosomal subunit protein uS10
    • Protein or peptide: Small ribosomal subunit protein eS17
    • RNA: 23S rRNA
    • RNA: 5S rRNA
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein uL5
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: Large ribosomal subunit protein eL8
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: Large ribosomal subunit protein uL14
    • Protein or peptide: Large ribosomal subunit protein uL15
    • Protein or peptide: Large ribosomal subunit protein eL15
    • Protein or peptide: Large ribosomal subunit protein uL18
    • Protein or peptide: Large ribosomal subunit protein eL18
    • Protein or peptide: Large ribosomal subunit protein eL19
    • Protein or peptide: Large ribosomal subunit protein eL20
    • Protein or peptide: Large ribosomal subunit protein eL21
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein eL24
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • Protein or peptide: Large ribosomal subunit protein eL30
    • Protein or peptide: Large ribosomal subunit protein eL31
    • Protein or peptide: Large ribosomal subunit protein eL32
    • Protein or peptide: Large ribosomal subunit protein eL43
    • Protein or peptide: Large ribosomal subunit protein eL37
    • Protein or peptide: Large ribosomal subunit protein eL39
    • Protein or peptide: Large ribosomal subunit protein eL40
    • Protein or peptide: Large ribosomal subunit protein eL42
    • Protein or peptide: Putative zinc finger domain-containing protein
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Methanosarcina acetivorans 70S ribosome

SupramoleculeName: Methanosarcina acetivorans 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#58
Source (natural)Organism: Methanosarcina acetivorans (archaea)

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Macromolecule #1: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 478.373938 KDa
SequenceString: AUUCUGGUUG AUCCUGCCAG AGGUUACUGC UAUCGGUGUU CGCCUAAGCC AUGCGAGUCA UAUGUUCUUU GUGAACAUGG CGUACUGCU CAGUAACACG UGGAUAACCU GCCCUUGGGU CCGGCAUAAC CCCGGGAAAC UGGGGAUAAU ACCGGAUAAC G CACAUAUG ...String:
AUUCUGGUUG AUCCUGCCAG AGGUUACUGC UAUCGGUGUU CGCCUAAGCC AUGCGAGUCA UAUGUUCUUU GUGAACAUGG CGUACUGCU CAGUAACACG UGGAUAACCU GCCCUUGGGU CCGGCAUAAC CCCGGGAAAC UGGGGAUAAU ACCGGAUAAC G CACAUAUG CUGGAAUGCU UUCUGCGUAA AACGGAUUCG UCUGCCCAAG GAUGGGUCUG CGGCCUAUCA GGUAGUAGUG GG UGUAACG UACCUACUAG CCGACAACGG GUACGGGUUG UGAGAGCAAG AGCCCGGAGA UGGAUUCUGA GACAUGAAUC CAG GCCCUA CGGGGCGCAG CAGGCGCGAA AACUUUACAA UGCGGGAAAC CGUGAUAAGG GGACACCGAG UGCCAGCAUC AUAU GCUGG CUGUCCGGAU GUGUAAAAUA CAUCUGUUAG CAAGGGCCGG GCAAGACCGG UGCCAGCCGC CGCGGUAACA CCGGC GGCC CGAGUGGUGA UCGUGAUUAU UGGGUCUAAA GGGUCCGUAG CCGGUUUGGU CAGUCCUCCG GGAAAUCUGA CAGCUC AAC UGUUAGGCUU UCGGGGGAUA CUGCCAGACU UGGAACCGGG AGAGGUAAGA GGUACUACAG GGGUAGGAGU GAAAUCU UG UAAUCCCUGU GGGACCACCU GUGGCGAAGG CGUCUUACCA GAACGGGUUC GACGGUGAGG GACGAAAGCU GGGGGCAC G AACCGGAUUA GAUACCCGGG UAGUCCCAGC CGUAAACGAU GCUCGCUAGG UGUCAGGCAU GGCGCGACCG UGUCUGGUG CCGCAGGGAA GCCGUGAAGC GAGCCACCUG GGAAGUACGG CCGCAAGGCU GAAACUUAAA GGAAUUGGCG GGGGAGCACA ACAACGGGU GGAGCCUGCG GUUUAAUUGG ACUCAACGCC GGACAACUCA CCGGGGACGA CAGCAAUAUG UAGGCCAGGC C GAAGACCU UGCCUGAAUC GCUGAGAGGA GGUGCAUGGC CGUCGCCAGU UCGUACUGUG AAGCAUCCUG UUAAGUCAGG CA ACGAGCG AGACCCGUGC CCACUGUUAC CAGCAUGUCC UCCGGGACGA UGGGUACUCU GUGGGGACCG CCGGUGUUAA AUC GGAGGA AGGUGCGGGC CACGGUAGGU CAGUAUGCCC CGAAUUUCCC GGGCUACACG CGGGCUACAA UGAAUGGGAC AAUG GGUCC CUCCCCUGAA AAGGGCUGGU AAUCUCACAA ACCCAUCCGU AGUUCGGAUC GAGGGCUGUA ACUCGCCCUC GUGAA GCUG GAAUCCGUAG UAAUCGCGUU UCAAUAUAGC GCGGUGAAUA CGUCCCUGCU CCUUGCACAC ACCGCCCGUC AAACCA CCC GAGUGAGGUA UGGGUGAGGG CACGGACUUC GUGCCGUGUU CGAACCUGAA UUUUGCAAGG GGGGUUAAGU CGUAACA AG GUAGCCGUAG GGGAAUCUGC GGCUGGAUCA CCUCCU

GENBANK: GENBANK: NR_074110.1

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Macromolecule #26: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 26 / Number of copies: 1
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 937.66775 KDa
SequenceString: AGGAAUUAUG CUAUCAGGUG GAUGGCUCGG CUCAAGAGCU UAUGAAGGAC GUGCCAAGCU GCGAUAAGCC CGGGGUAGGU GCAUGGAGC CAAUGAACCC GGGAUUUCCG AAUGGGACCU CUCCGUAGUG AUCAUUCAUG AUCGGGAACG CUCCGAAUUG A AACAUCUC ...String:
AGGAAUUAUG CUAUCAGGUG GAUGGCUCGG CUCAAGAGCU UAUGAAGGAC GUGCCAAGCU GCGAUAAGCC CGGGGUAGGU GCAUGGAGC CAAUGAACCC GGGAUUUCCG AAUGGGACCU CUCCGUAGUG AUCAUUCAUG AUCGGGAACG CUCCGAAUUG A AACAUCUC AGUAGGAGCG GGAAAAGAAA UCAAUUGAGA UGCCGUAAGU AACGGCGAGU GAAACCGGCA CAGUUCAAAC CG AAUCCCU UCGGGGACAU GUGGUGUUGU AGGGCUGUUC AAACGUCUGG CGGUAAAAUC GAACUUGCCU GGAACGGCAG ACC AUAGAG UGUGACAGUC ACUUAGAUGU ACGCCAAAAG ACCGGAACAU GUCCCUGAGU ACCGUGCGUU GGAUAUCGUG CGUG AAUCU GGGGGGCACC AACCUCCAAA ACUAAAUACU CCUUGAGACC GAUAGCGAAA UAGUAGGGUG ACCGAACGCU GAAAA GUAC CCCGAGAAGG GAGGUGCAAA GUGCCUGAAA CCUGAUAGUG AUGGAACGAU ACGGCAUGAA AGGAUCUCUG GCAUGA AGG AAUCAUUCGC GAGGAUGUAG UACGAAUGUC ACUGCCAGUG UCGUAUCUUA CGUUUUGAAG AACGGGCCAG GGAGUGU AU CUUGGUGGCA AUGGCUAACC UUUUAUCUGG GCAGCCGAAG CGAAAGCAAC AUGUGCGCAG CCUUUUGGCG AGGCACGA C GUAUACAAGU GCGUGGAGUC ACCGGGGUAC GACCCGAAGC CGGGUGAUCU AGGCGUGGGC AGGUUGAAGC GUGGCGAAA GCUACGUGGA GGACCGUAAA CGGUAUUGAU CUGCAAAUCA UUCGUGUGAC CUGCGUCUCG UAGUGAAAUG CUAAUCUAAC CCGGCAUCC GCUGGUUCCU UCCGAAACAU GUCGCAGCAU GACCUGACUU GAGAUCGUCG GUGGAGUAGA GCACUGAUUG G CGAUUCCG GGGGAGAAAU CCCUCGCUCG CUUGUCAAAC UCCAAACCCC CCGUCAUCCU AGAAAGUCGG AGUCCGGACU GC UGGGGUA AGCUUGUAGU CCGUAAGGGA GACAACCCAG CCCGUGGUUA AGGUCCCCAA GUGUCGACUA AGUGUUAAUA CUA AAGGGC GUCCCAAGCC CCAGACAGCU GGAAGGUUAG CUUAGAAGCA GCUACCCUUC AAAGAGUGCG UAACAGCUCA CCAG UCGAG GUUUGGGGCC CCGAAAAUUG ACGGGGCUCA AGUCGACCAC CGAUACCACG GAGUACCGCA AGGUAAUCUC GUAGG AAGG CGUUGUGUUC GGGUCGAAGC AGGGCUGUGA AGUCCUGUGG ACCGUUCACA AACGAAAAUC CUGGUAAUAG UAGCAG CAA AGCAGGGUGA GACUCCCUGC CGCCGAAGGG GCCAGGUUUC CUCGGCAAUG UUCGUCAGCC GAGGGUUAGU CGGUCCU AA GACGUACCGU AAUUCGAGUA CGCCGAAAGG GAAACAGGUU AAUAUUCCUG UACCAUUUAG CGUUUAGUCU GACGUCUC A GGGCAAGCCG AGCGGCGUCG UCGCGCCGUC UAAGCAGCGA ACCCCGUGGA GAGCCGUAAU GGCGAGAAGC GGGCGAAUC UGUAAUGGCA CAAGUCGGCU UCACCCCGGG ACCCGUGAAA AGGACAGCUA AAUGUCCGUA CCGAGAACUG ACACAGGUGC CCCUAGCUG AAAAGGCUAA GGCGUGUCGG AGUAAUUCAG UUAAGGGAAU UCGGCAAAUU AGCUCCGUAA CUUCGGGAUA A GGAGUGCC UGCUGUGUAG ACAGCAGGUC GCAGUGACCA GGGGGCUCUA ACUGUCUAAU ACCAACAUAG GAGAUUGCAA AC CCGUAAG GGCUAGUACA AUCUCUGAAU CCUGCUCAGU GCAGGUACCU GAAACCCCGG UUCAACGGGA AGAAGGGCCU GUA AACAGC GGGGGUAACU AUGACCCUCU UAAGGUAGCG UAGUACCUUG UCGCUUAAUU GGCGACUUGC AUGAAUGGAU CAAU GAGAG CCCUACUGUC CCUAACUGGA GUCCGGUGAA GCUUACAUUC UAGUGCACAG UCUAGAGACC UCUAGGGGGA AGUGA AGAC CCCGUGGAGC UUUACUGCAG CCUGUCGUUG GGUUGUGGUU CUGGAUGUAC AGUGUAGGUA GGAGGCAUCG AAGCGG GUG CGCCAGCAUU CGUGGAGCCG UCCUUGGGAC ACUACCCUUC CGGGACUACG ACCCUAACUC UGAGAAGAGG ACCCCGA UA GGUGGGCAGU UUGCCUGGGG CGGGACGCCC UUGAAAAGAU AUCAAGGGCG CGCAAUGGUU GACUCAAGCG GGUCGGAA A CUCGCUGAAG AGUGCAAGAG CAUAAGUCAG CCUGACGUUA UUCAGCAUAG CAGUGGAUGA CGAGACGAAA GUCGGCUCU AGCGAACUUU UGAGCCUCCU UGGUGGGGGC CAAAAAUGAC AGAAAAGUUA CCCCGGGGAU AAUUGAGUCG UUGCCGGCAA GAGUACAUA UCGACCCGGC AGCUUGCUAC CUCGAUGUCG GUUCUUUCCA UCCUGGCCGU GCAGCAGCGG CCAAGGGUGA G GUUGUUCG CCUAUUAAAG GAGAUCGUGA GCUGGGUUUA GACCGUCGUG AGACAGGUCG GUUACUAUCU ACUAGAGGUG CC CGAGGUC UGCGGGUAAG CUGCUUUUAG UACGAGAGGA ACCAAGCAGC GGCGCCACUG GUGUACCGGU UGUCUGACAA GGC AUCGCC GGGCAGCUAC GCGCUCAGGA AUAAGAGCUG AAUGCAUCUA AGCUCGAACU CUGACCUAAA AAGAGACCUC UUUA AGGAU UCCGGUAAAA GACCGGUUUG AUAGAAACGG GAUGUAAGCA CCAAGGCAAC GAGGUGUUCA GUCCGCGUUU ACUAA CCAU UCGUUCCUUU CACUU

GENBANK: GENBANK: AE010299.1

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Macromolecule #27: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 27 / Number of copies: 1
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 41.399551 KDa
SequenceString:
GAGUUUGGCG GCCAUAGCGG UAGGGCAACU CCUGUACCCA UCCCGAACAC AGCAGAUAAG CCUGCCCGCG UUCCUUACUG UACUGAAGU GUGCGAGCCU UCGGGAACUC UGGAUCGCUG CCAAGCUCAC

GENBANK: GENBANK: AE010299.1

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Macromolecule #2: Small ribosomal subunit protein uS2

MacromoleculeName: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 27.23458 KDa
SequenceString: MEEIEQTGQE ETGAQPMPEE DVVAEAKPAL EKEAAVKTGS IPSESEDETA SHKEGSTSLV SIDEYLAAGV HIGTQQKTQD MMRFVYRVR TDGLYVLDIQ STDERIRVAS KLLSHYDPAR ILVVSSRQYG QHPARMFSRA LGTRAMLGRF IPGSLTNPQI H GFFEPDVI ...String:
MEEIEQTGQE ETGAQPMPEE DVVAEAKPAL EKEAAVKTGS IPSESEDETA SHKEGSTSLV SIDEYLAAGV HIGTQQKTQD MMRFVYRVR TDGLYVLDIQ STDERIRVAS KLLSHYDPAR ILVVSSRQYG QHPARMFSRA LGTRAMLGRF IPGSLTNPQI H GFFEPDVI IVTDPAGDAQ VLKEASSIGV PVVALCDTNN LTSNVDLVIP TNNKGRKALS LVYWLLAREV SRLNDTPFNY EL TDFETPL

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: Small ribosomal subunit protein uS3

MacromoleculeName: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 34.780445 KDa
SequenceString: MAIEKKFVND GYVKASMDEY FAEQLSRAGY GGMELNRTPM GTQIVIYSEK PGMVIGKAGK VIRKLTRDVA NKYSLENPQI DAQEVKRPE LNAQMMASRL AASIERGWYF RKAGHNTLRA VMNAGALGCE VVISGKLTGA RSRVEKFVDG YIKHSGNPVE E VVDEGFAV ...String:
MAIEKKFVND GYVKASMDEY FAEQLSRAGY GGMELNRTPM GTQIVIYSEK PGMVIGKAGK VIRKLTRDVA NKYSLENPQI DAQEVKRPE LNAQMMASRL AASIERGWYF RKAGHNTLRA VMNAGALGCE VVISGKLTGA RSRVEKFVDG YIKHSGNPVE E VVDEGFAV AVKKLGTLGC KVRIIQPGVV LPDSYKVRES VEVEEPAEKP AEKPAEKPAE KAAAPKKEAA KARAPAPAPE AP APAPEAP APAPVEEAEV AEPEEAEEVQ AETSEEIEGA ELVYVEGSDE VRRQVNGVWQ HKHGSYDYWH PMARVHKEAK E

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #4: Small ribosomal subunit protein eS1

MacromoleculeName: Small ribosomal subunit protein eS1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 22.938652 KDa
SequenceString: MARKKVQRKL DGWKSKEWYN IEAPVYLNRA IVGNTMAGDP SLLVGRNVET TVGELTNDMT KNNTKVILRI NNVVGDIATT DLMGHELTT DYIRSIVKRQ TSRIDANVDV KTKDGYVIRV KPTCFTIKRA RSSQMQAIRE MMVEIVKKRA AESDFETFMQ E AILGRLSA ...String:
MARKKVQRKL DGWKSKEWYN IEAPVYLNRA IVGNTMAGDP SLLVGRNVET TVGELTNDMT KNNTKVILRI NNVVGDIATT DLMGHELTT DYIRSIVKRQ TSRIDANVDV KTKDGYVIRV KPTCFTIKRA RSSQMQAIRE MMVEIVKKRA AESDFETFMQ E AILGRLSA AIYRQAKFIY PLRRVEIRKT EVETIPAAAP APAAA

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #5: Small ribosomal subunit protein uS4

MacromoleculeName: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 24.559277 KDa
SequenceString: MAYPGKKSKS YETPKHPWQE ARMATEVQLV KAYGLRNKKE VWKAASMLRM YRSEARKLLA HVASGQEGGL EGHYRTQSEE ILSKLIRYG IIKSDANIDD ILSLKTENIL ERRLQTQVLR LGLARTVIQA RQFITHGHIA INGRKATIPG MLVSKEDEMH I GYYGNSPL ...String:
MAYPGKKSKS YETPKHPWQE ARMATEVQLV KAYGLRNKKE VWKAASMLRM YRSEARKLLA HVASGQEGGL EGHYRTQSEE ILSKLIRYG IIKSDANIDD ILSLKTENIL ERRLQTQVLR LGLARTVIQA RQFITHGHIA INGRKATIPG MLVSKEDEMH I GYYGNSPL KNESHPERPV QVASFLADSG TTLKAAAEAK QAREKPPERG GGRRKRGGRR

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #6: Small ribosomal subunit protein eS4

MacromoleculeName: Small ribosomal subunit protein eS4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 26.301408 KDa
SequenceString: MTHQKRLSVP KSWKVGKKGN KWISTTRPGP HSQARSLPLG IIIRDILKLV DNSREGKRIL SEGKVLVDGI PRKDLRFPVG LFDVITLPL VNEAYRMLQD EKGRLTLHKL NETNVNKLCR INNKTTVKGG KFQLNLNDGT NIIGSNEYGT KDSLILSIPD K QVVKHLKF ...String:
MTHQKRLSVP KSWKVGKKGN KWISTTRPGP HSQARSLPLG IIIRDILKLV DNSREGKRIL SEGKVLVDGI PRKDLRFPVG LFDVITLPL VNEAYRMLQD EKGRLTLHKL NETNVNKLCR INNKTTVKGG KFQLNLNDGT NIIGSNEYGT KDSLILSIPD K QVVKHLKF EVGNLAMVVG GQHSGETGKI MEIREVKSSR HNTVMISGET DFETIEDYVI VIGEDKPEIR LGGEISE

UniProtKB: Small ribosomal subunit protein eS4

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Macromolecule #7: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 22.732264 KDa
SequenceString: MAFDQDWVPK TRLGKLVVEG QVASMDEAIK SGLPIREPQI IDMLLPDLED EVLDINMVQR MTDSGRRVKF RATVIVGNRN GYVGLGQAK DVQVGPAIRK AIDAAKLNIT YIRRGCGSWE CACGLPHTVP YEVTGKAGSV SVTLIPAPRG LGIAAGNTAT K VLEKAGIK ...String:
MAFDQDWVPK TRLGKLVVEG QVASMDEAIK SGLPIREPQI IDMLLPDLED EVLDINMVQR MTDSGRRVKF RATVIVGNRN GYVGLGQAK DVQVGPAIRK AIDAAKLNIT YIRRGCGSWE CACGLPHTVP YEVTGKAGSV SVTLIPAPRG LGIAAGNTAT K VLEKAGIK DVWTKTFGTT RSTLNFAKAT YDALNQVNVV RLPVYYGKEE V

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #8: Small ribosomal subunit protein uS7

MacromoleculeName: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 21.06235 KDa
SequenceString:
MIFLYKIFGK WDPTEVEVKD LGIKRYVSLT PVIVPHSSGK HARQQFNKSE ISIVERLANN LMRTETNTGK KQVTLRAVEE AFDIVNKKT KQNPIQVLVD AIANAGPREE VVRLKYGGIS VPKAVDTAPQ RRVDTALRYI SMGTNAAAFK SKRSVAECLA T ELIGAANR DTKSFSINRK DAKERVAKAA R

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #9: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 14.085301 KDa
SequenceString:
MVLLDPLANA LSTIKNAEAI GKSSCVVRPA SKNIGNVLKV MQDLGYIGDF EFIDDGKAGI YSVTLVGRIN KCGAIKPRYS VGTASFERW EKQFLPAKNF GALIVTTSSG VMSQYEARDK KIGGQLLAYV Y

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #10: Small ribosomal subunit protein eS8

MacromoleculeName: Small ribosomal subunit protein eS8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 13.801993 KDa
SequenceString:
MRWQGSSRRK ATGGKVITAR GKRKFEMGRE SAETRISEIK RKKVPTMGGN RKVRLLQSNV ANVTNPKDGK TVTAPIETVI DNTANKHYV RRNILTKGSV IRTSMGTARV TSRPGQDGVV NAVLIE

UniProtKB: Small ribosomal subunit protein eS8

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Macromolecule #11: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 14.512666 KDa
SequenceString:
MVKVVNSSGK HKTATARATV MKGTGKVRIN KIPLELYTPE LAMMKISEPL LIAGNDVVSG LDINVDVRGG GIIGQANAVR TAVARGIVE WTNDTAIRDN FAAYDRNLLV SDSRQKESKN FGGPGARAKY QKSYR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #12: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 13.324279 KDa
SequenceString:
MADMKWAVAH IKSSFNNTII TVTDITGAET IAKSSGGMVV KAARDESSPY TAMQMAGQLA DQLKDKGING IHIRVRAPGG NKQRSPGPG AQAAIRAFAR AGIRIGRIED VTPVPHDGTR PKGGRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #13: Small ribosomal subunit protein uS12

MacromoleculeName: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 15.54014 KDa
SequenceString:
MAKGKYAANI LKQTRKDARW KDTYYGRRVL GLNVKADPLG GAPQGRGIVL EKVGVEAKQP NSAIRKCVRV QLIKNGRQVT AFCPGDGAV NFIDEHDEVT VEKIGGRMGG AMGDIPGVRF KVIAVNNVSL NQLVIGRMEK PRR

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #14: Small ribosomal subunit protein uS13

MacromoleculeName: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 20.693932 KDa
SequenceString:
MRAAPEFSAY SLRVFDRGYR MLYALQWRKY MVEENNNEEL RHLVRIMNTD LQGAKPVQYA LTGLPGVGRR TAKLIAKGAG VDPSAILGY LPEEEVAKLD TAIGKFEDIV PSWMLNRQKD LATGQDKHLL GTDILLTFRE DINNLKKVRA YRGLRHERGL K VRGQRTKS TGRRGSTVGV SRKK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #15: Small ribosomal subunit protein uS14

MacromoleculeName: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 5.971021 KDa
SequenceString:
MTETIKKSGR GVNECKRCGR KQGLVRKYDI YLCRHCFREI AHEMGFEKYS

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #16: Small ribosomal subunit protein uS15

MacromoleculeName: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 17.503594 KDa
SequenceString:
MAKMHTKRKG KSSSTRPIRT DPPEWCKIGA DEVTTIVLDL WKQGVSTAEI GMVLRDRYGV PDAKLITGKK VTTILKENNV APNIPEDLT NLIVKALGLR KHLSVNKKDV HNKRSLNLTE SKIRRLVKYY KQEKVLPRDW FYKPETAEMM ITR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #17: Small ribosomal subunit protein uS17A

MacromoleculeName: Small ribosomal subunit protein uS17A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 12.184246 KDa
SequenceString:
MARDIGLNIP APSEECDDAY CPFHGTLPVR GQILVGTVVS SKMDNTVVIE RQYMKMVSKY QRYEKRRSKI HAHNPACISA KVGDIVTIA ECRPISKTKS YVVVKAEVPK

UniProtKB: Small ribosomal subunit protein uS17A

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Macromolecule #18: Small ribosomal subunit protein uS19

MacromoleculeName: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 15.467917 KDa
SequenceString:
MAKKSSSRLP KRKGEYTYRG KTVSELQELS LEEFAELLPS RERRSLKRGF TDGQKKVLHE FKEGKKIRTH HRDMIILPEM IGKTIEIHN GKGFVSVDLQ PEMVGHRFGE FAPTRSRVSH GSAGVGATRS SKFVPLK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #19: Small ribosomal subunit protein eS19

MacromoleculeName: Small ribosomal subunit protein eS19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 16.563168 KDa
SequenceString:
MTTVFDVPAT EMIEKLAGIL KEDDKIVPPE WAGNVKTGVH KELPPTNDDW WYIRCAAVLR KIYTDGPIGI ERLRSVYGGK KDNGSKPFH KAKGSGSVAR KTVQQLEAAG FLQKVKDGRT VSAKGRSMMD NAAHELKLEL LEKIPELAKY

UniProtKB: Small ribosomal subunit protein eS19

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Macromolecule #20: Small ribosomal subunit protein eS24

MacromoleculeName: Small ribosomal subunit protein eS24 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 11.620263 KDa
SequenceString:
MDIKILKDKN NSLLNRRELD FVVKYEGSTP SRSDVRNKLA AMLNAPLELL IIQRIKTEYG MQESKGYAKV YADEARMKQV EQEYILKRN PAPGAEAEEE EA

UniProtKB: Small ribosomal subunit protein eS24

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Macromolecule #21: Small ribosomal subunit protein eS27

MacromoleculeName: Small ribosomal subunit protein eS27 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 6.955049 KDa
SequenceString:
MVDYTQRPKS RFLRVKCNDC ENEQIIFGSA SRKITCVVCG RTLAEPTGGK STITTHILEV LE

UniProtKB: Small ribosomal subunit protein eS27

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Macromolecule #22: Small ribosomal subunit protein eS28

MacromoleculeName: Small ribosomal subunit protein eS28 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 8.268643 KDa
SequenceString:
MLIMAEESTI GGFAAEVIDV IGNTGMHGEA SQIQCRVLEG RDKGRVITRN CVGPVRIGDI LMLLETSREA KKLTTR

UniProtKB: Small ribosomal subunit protein eS28

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Macromolecule #23: Small ribosomal subunit protein eS6

MacromoleculeName: Small ribosomal subunit protein eS6 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 14.474798 KDa
SequenceString:
MANFKVVVSD PKEGRAYQID IKDAEANALI GKAIGDVVDG AIFGLAGYKV QITGGCDGSG FVMKPDLPGP RRQRILTATG VGYVPKLPG QRRRKMMRGK EIAPDIIQVN AKIVEYGSKS IKALLGLETA EEAPAAE

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #24: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 11.38028 KDa
SequenceString:
MQKARIRLSG ISPKDLDGVC NQVKSIAERT GVNISGPVPL PTKKLVVPTR KSPSGDGTAT WDHWEMRVHK RLIDIAADER ALRQLMRIQ VPKDINIEIV LEG

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #25: Small ribosomal subunit protein eS17

MacromoleculeName: Small ribosomal subunit protein eS17 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 7.477688 KDa
SequenceString:
MGNIRETNIK RTAFSLLENY GDVFTKDFET NKALVTKYTT IESKIIRNRV AGYVTRKVAR MKVY

UniProtKB: Small ribosomal subunit protein eS17

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Macromolecule #28: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 25.738627 KDa
SequenceString: MGKRLISQNR GRGTPTYRAP SHKYKADLRH PRVDENSSLR GEVVGIEHDP ARSAPIAKVA FENGEELFLL ASEGIAVGNI IECGDDAEV KPGNIVPIGN VPEGFFICNV ESKPNDGGKF VRSSGVYATV VTHEATRTAV SMPSGNIKWL NPKCRAVVGI V AGSGRVDR ...String:
MGKRLISQNR GRGTPTYRAP SHKYKADLRH PRVDENSSLR GEVVGIEHDP ARSAPIAKVA FENGEELFLL ASEGIAVGNI IECGDDAEV KPGNIVPIGN VPEGFFICNV ESKPNDGGKF VRSSGVYATV VTHEATRTAV SMPSGNIKWL NPKCRAVVGI V AGSGRVDR PWLKAGKKYH KMKTRAAKYP RVSAVAMNPR DHPFGGGAWK HPGKPTTVSR NAPPGRKVGL IAARRTGMKR

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #29: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 36.949645 KDa
SequenceString: MASIHRPKRG SLAFSPRKRA KSHIPRFRAW PEATGEPKLQ SFAGYKVGMT HVIMVDDTKN SLTQGMEISV PVTVIETPAI RVAAIRAYA EDSTGEKAIA EVWAADLDPE LKRRIPIPAA GNQAEALENI GKLIEEGRVS DVRAVIYTLP KSLTGVPKKV P DIMESGIS ...String:
MASIHRPKRG SLAFSPRKRA KSHIPRFRAW PEATGEPKLQ SFAGYKVGMT HVIMVDDTKN SLTQGMEISV PVTVIETPAI RVAAIRAYA EDSTGEKAIA EVWAADLDPE LKRRIPIPAA GNQAEALENI GKLIEEGRVS DVRAVIYTLP KSLTGVPKKV P DIMESGIS ARDLGTKFEY SKTILGTLVS VTDVFKNGTL VDTAAITIGK GTQGPVKRWG IQLMKGKHSR QGSLRQVGTL GA FNPSRVS WRVPQMGQMG YHQRTEFNKR ILKIGSDGEE VTPEGGFINY GLVRGDYILI KGSVPGPSKR LIRLRDPIRA KKA DLGEPN ILYISRESKQ G

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #30: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 27.51657 KDa
SequenceString: MATAKTIDLT GKAVGEVELP AVFDADYRPD LIKKAVLAAQ ANRLQPYGPR LYSGMETSAR GWGSGRGVSH VPRLVNSSRA ARVPHAKGG RRAHPPKPEA DRSEKVNTKE RRYAIRSAIA ATTDPTLVSL RGHIFEAELP IVAVNDLESL ERTKQVIEFL E AAGLYEDV ...String:
MATAKTIDLT GKAVGEVELP AVFDADYRPD LIKKAVLAAQ ANRLQPYGPR LYSGMETSAR GWGSGRGVSH VPRLVNSSRA ARVPHAKGG RRAHPPKPEA DRSEKVNTKE RRYAIRSAIA ATTDPTLVSL RGHIFEAELP IVAVNDLESL ERTKQVIEFL E AAGLYEDV LRAKYGRHIR AGRGKLRGRK YKHKKSVLIV AGENTPILKA ARNLSGVDVV TVDSLNAELL APGTHAGRLT VW TESAIGK LEGAFQ

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #31: Large ribosomal subunit protein uL5

MacromoleculeName: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 18.398299 KDa
SequenceString:
MRTPIVEKVI VHMGVGESGQ HLVNAEDILR NITGQEVVRC FAKRTLPAFS IKKNEPIGCK VTLRGQKAQE FLETALGIVE KTLNRSQFD SFGNVSFGIE EHTDFPGMRY DPNIGVFGMD VTVVLKRPGE RICKRRIAAR KIPAGHRVTV DDAIAFLNES Y GVEVM

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #32: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 19.530715 KDa
SequenceString:
MVKEIARTIE IPEGVSVSLA QDVFTATGPK GTVERKLWYP GIMIDVKDGE VVVDAEYARK EQKAMVGTFA SHIRNLVKGV NEGFECKMS IVYAHFPMQV KVDGKTLIIG NFLGEKKPRF AKIIGETKVK VSGNDVTITG INKEDVGQTA ANIEQKTKIK R FDPRIFQD GIYIVQKA

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #33: Large ribosomal subunit protein eL8

MacromoleculeName: Large ribosomal subunit protein eL8 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 12.741726 KDa
SequenceString:
MAQLAKFDVP EELTNKALEA LELARDTGKI KKGTNEATKA IERGNAKLVL IAEDIEPAEI VAHIGPLSEE KKAPYIFIKN QKELGAASG LGVSCATVAI VDAGKAAEMV QDIAQKLEAL K

UniProtKB: Large ribosomal subunit protein eL8

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Macromolecule #34: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 19.409648 KDa
SequenceString:
MVRKPGSMYR NVRQRSFTRR KYMGGVPGSQ VIHYDMGDKA NTSFPVKISL IVEEKCQIRH TALEAARITA NRHLVADTGK MGFYMKLRV YPHEVIRENK QATGAGADRV SSGMRRAFGK NVGTAARVKP MQKIFTVAVE KQNFKAAKEA LWHAGQKLPT P CRIVVDEG AELVQ

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #35: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 16.085799 KDa
SequenceString:
MLKMTVIDAK GLILGRLASS VAKQLLSGDE KVYIINAEQA IISGSRAATL REYRETRERG ATEFGPYFPK RPDRILKRTI RGMLPYKRA RGRDAMSRLK VYVGVPYELK GAETVTIPDA DMRLLSSSRY VELGEVSQKM GSKF

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #36: Large ribosomal subunit protein uL14

MacromoleculeName: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 14.356907 KDa
SequenceString:
MKGMRSNIPR ALNAGAQIAC VDNTGAKVVE IISVKKYRGV KNRMPCAGIG DMCVVSVKKG TPEMRKQVLL AVVVRQKQEF RRPDGLHVS FEDNAMVITD EEGIPKGTDI KGPVAREVAE RFPKIGTTAS IIV

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #37: Large ribosomal subunit protein uL15

MacromoleculeName: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 15.129009 KDa
SequenceString:
MDTKKFRGSR TCGGGTHKNR RGAGNRGGRG KAGGCKHHFV RAMMRGYSYG KHGFKRPDEV SRDVSIVNVG ELDELASYLV EEGLAEVKD GAYHINLENL GIEKVLGSGR VTKNLVVTSE EFSASAREKI ENAGGSCIDA E

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #38: Large ribosomal subunit protein eL15

MacromoleculeName: Large ribosomal subunit protein eL15 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 22.650105 KDa
SequenceString: MVKSFYGYVR DAWKNPDETY VNELRWERLQ VWRKQGSVTR IERPTRIDRA RSLGYKAKQG IVVVRVNVRR GGLGHVRPNR GRRTQKMGK NKVSGGMSIQ RIAEVRADRR YPNLEVLNSY WVGEDGKHKW FEVILVDPHH PVIKSDKNLN WVCDPSSRGR A TRGKTSAG ...String:
MVKSFYGYVR DAWKNPDETY VNELRWERLQ VWRKQGSVTR IERPTRIDRA RSLGYKAKQG IVVVRVNVRR GGLGHVRPNR GRRTQKMGK NKVSGGMSIQ RIAEVRADRR YPNLEVLNSY WVGEDGKHKW FEVILVDPHH PVIKSDKNLN WVCDPSSRGR A TRGKTSAG RKGRGMATRG KGTEKTRPSI RAYKSRGK

UniProtKB: Large ribosomal subunit protein eL15

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Macromolecule #39: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 19.233713 KDa
SequenceString:
MATGPRYKVP FRRRREGRTN YHLRLKLLLS RQDRVVVRKS SRNVQIQLIA PTPDGDITYS SAVSNELAKY GYTGATGNTT AAYLTGLLF GLKSLQKGYE GGILDIGLQA SSAGSRVYAA LKGVVDSGFE IPCSPEVFPP DERIRGEHIA GYREESSDLP E QFEATKEK IFAEFS

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #40: Large ribosomal subunit protein eL18

MacromoleculeName: Large ribosomal subunit protein eL18 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 13.714989 KDa
SequenceString:
MGKKSLVKLT RKTNPRIVSL ILTLKERANG DSAPIWKDIA KRLEAPSRNY AAVNISKINR HTAEDDVLLI PGKVLGAGLL DHPVTIAAL TFSDSAFEKI TEAGGKCLSL EEIMEANPKG SGIRIFR

UniProtKB: Large ribosomal subunit protein eL18

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Macromolecule #41: Large ribosomal subunit protein eL19

MacromoleculeName: Large ribosomal subunit protein eL19 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 17.061982 KDa
SequenceString:
MSDLSNQRKL ASKVLGCGLD RVWLNPEASE EIASAITRED IRGLIEEGTI KAKPVKGVSR GRARALAAKR KYGHCKGHGS RKGKKGART PKKEQWIKKI RALRRRLKEL RADGALDKSV YCRLYRKAKG GEYRSVSHLN SHLESEKLLK KE

UniProtKB: Large ribosomal subunit protein eL19

+
Macromolecule #42: Large ribosomal subunit protein eL20

MacromoleculeName: Large ribosomal subunit protein eL20 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 6.972045 KDa
SequenceString:
MVMMKSFIVK GKFKAGSTWE KFTKKIESQN EKNATDKTYS IFGSKHGVKR SQVQIESVAE E

UniProtKB: Large ribosomal subunit protein eL20

+
Macromolecule #43: Large ribosomal subunit protein eL21

MacromoleculeName: Large ribosomal subunit protein eL21 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 11.06372 KDa
SequenceString:
MTNSHGEKRC TRYKLQKTVR ERGISPVSRA IQEFEEGQMV HIDIDPSVQK GMPNPKFQGS TGKVIGQRGR SYVLEVRSGN AMKEVFSLP QHLKPQKY

UniProtKB: Large ribosomal subunit protein eL21

+
Macromolecule #44: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 16.764578 KDa
SequenceString:
MARINYSING DPETTSKAMG SELHISPKKS REVCCKIKGM KASEARKFLE DVIAMKQAVP FKKHHDGSGH RKGPMAAGKY PISASKEIL KVLKNAESNA EYKGLEPANM YIIHAAIQRG RVIHGFMPRA RGRATPKDTE TVNIEMILSE VR

UniProtKB: Large ribosomal subunit protein uL22

+
Macromolecule #45: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 9.447194 KDa
SequenceString:
MSSINYPFVT EKAMMLLDEN KLQFIVDTRS NKKQILEDVE KMYGFKVKSV RTMTTMKGMK KAVLAFEEPE AAHEIATRIG LM

UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #46: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 13.075341 KDa
SequenceString:
MIAMVSKQPR KQRKARYAAP LHIRQKFMGA RLSEALTKEY GTRSAAVITG DTVKVMRGDF KGTEGKVQSV SLMDGTITVD GVISTKVDG TEVPRPLNPS NVMITKLEMK DGRRASSIKK

UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #47: Large ribosomal subunit protein eL24

MacromoleculeName: Large ribosomal subunit protein eL24 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 7.204596 KDa
SequenceString:
MEQRKCYFCG KMLEPGTGKL YVKKDGSTYF MCSSKCMSNF ALGRLPRRTE WTEKGKIQLK KA

UniProtKB: Large ribosomal subunit protein eL24

+
Macromolecule #48: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 7.622637 KDa
SequenceString:
MAILRTSEIR TMTIEERADE LENLNNELVR ERALTSAGGA PENPGRIGEI RRTIARIKTI QHELNEI

UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #49: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 17.627416 KDa
SequenceString:
MYAVVRLRGQ VNVRYTIEDT MKMLRLHKVN HCVFVPENPH YKGMVQKVKD YVAYGKIDAK TLAEVLENRG RLEGDTRLTE EYIRENTDY DSIQAFAEAV IEGKSSLKDI PKLKPVFRLH PPRKGHAGIK RTVQQGGVLG NHDENINVLL HKMR

UniProtKB: Large ribosomal subunit protein uL30

+
Macromolecule #50: Large ribosomal subunit protein eL30

MacromoleculeName: Large ribosomal subunit protein eL30 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 10.316188 KDa
SequenceString:
MMKMKINVDK SLIKAVKTGK VIVGANRTID AAANGSAKMV VLASNCPEDI KKKIQATDIP VLEYEGTSVE LGPVCGKPFT IAAMAILDV GESDILAATA

UniProtKB: Large ribosomal subunit protein eL30

+
Macromolecule #51: Large ribosomal subunit protein eL31

MacromoleculeName: Large ribosomal subunit protein eL31 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 10.331096 KDa
SequenceString:
MADDMVKEQI YTIPLREVRK VPAWKRANRA VIEVRGFLVR HMKTESVQVK LDKSINEHLW EKGCEKPPLS IRVRAVKFAD GEVQAELAQ

UniProtKB: Large ribosomal subunit protein eL31

+
Macromolecule #52: Large ribosomal subunit protein eL32

MacromoleculeName: Large ribosomal subunit protein eL32 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 17.880393 KDa
SequenceString:
MIMMAEEFNE VEGTNEVEDI NEVEGTSVST LDMDPESRRL FNVRKVQKGK KPQFKRTCSH KFKRLDDNWR RPRGSQGKQR RKYVSKGAL VQVGYGSPAA VKGLHPSGYS DVLISSIAEL ELVDPSYEAI RIAGTIGAQK KALILAKAEE AGIKVLNSGR S E

UniProtKB: Large ribosomal subunit protein eL32

+
Macromolecule #53: Large ribosomal subunit protein eL43

MacromoleculeName: Large ribosomal subunit protein eL43 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 10.557483 KDa
SequenceString:
MAKKFTKKGR ISRSAGRFGP RYGRKDRKLV ADLEERMRAP HVCTKCARPT VERIGTGIWK CSKCGHTFAG GTYIPYTSVG QTLLRTMKN IAEAK

UniProtKB: Large ribosomal subunit protein eL43

+
Macromolecule #54: Large ribosomal subunit protein eL37

MacromoleculeName: Large ribosomal subunit protein eL37 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 6.456628 KDa
SequenceString:
MSKGTSSMGK RQKRTHAKCR RCGSVSFNVH TKQCTSCGFG KTSRMRTYKW QAKCKY

UniProtKB: Large ribosomal subunit protein eL37

+
Macromolecule #55: Large ribosomal subunit protein eL39

MacromoleculeName: Large ribosomal subunit protein eL39 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 6.257516 KDa
SequenceString:
MSHNMKGQKK RLAKAHKQNT RVPVWVIVKT NRKVVSHPRR RHWRRRSLDV K

UniProtKB: Large ribosomal subunit protein eL39

+
Macromolecule #56: Large ribosomal subunit protein eL40

MacromoleculeName: Large ribosomal subunit protein eL40 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 5.964202 KDa
SequenceString:
MTKMARFPEA EERLLNKKIC MKCNARNAIR ATRCRKCGYG TLRVKSKESK GA

UniProtKB: Large ribosomal subunit protein eL40

+
Macromolecule #57: Large ribosomal subunit protein eL42

MacromoleculeName: Large ribosomal subunit protein eL42 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 10.872954 KDa
SequenceString:
MKIPKRFRTY CPFCKTHNEV VVERVKKGQA SSMTHIARQK KRQEGIGNSG KFSKVPGGDK PTKRIWLRYR CTVCKKAHQR PCFRAKKFE FKE

UniProtKB: Large ribosomal subunit protein eL42

+
Macromolecule #58: Putative zinc finger domain-containing protein

MacromoleculeName: Putative zinc finger domain-containing protein / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Methanosarcina acetivorans (archaea)
Molecular weightTheoretical: 6.16226 KDa
SequenceString:
MSSQKVEYCT SCGIRLVEKG YVKFPCPQCG AEIGRCISCR QQGNVYTCPK CGFKAP

UniProtKB: DUF1610 domain-containing protein

+
Macromolecule #59: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 59 / Number of copies: 51 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #60: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 60 / Number of copies: 9 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.5 mg/mL
BufferpH: 7.5
Component:
ConcentrationNameFormula
20.0 mMTris acetate
60.0 mMAmmonium chlorideNH4Cl
7.5 mMMagnessium acetateMg(CH3COO)2
6.0 mMbeta-mercaptoethanol
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC (ver. v4.6.2) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6skf

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.92 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. v4.6.2) / Number images used: 91385
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. v4.6.2)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. v4.6.2)
FSC plot (resolution estimation)

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