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- EMDB-48317: Near complete virion structure of bacteriophage PhiTE -

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Basic information

Entry
Database: EMDB / ID: EMD-48317
TitleNear complete virion structure of bacteriophage PhiTE
Map data
Sample
  • Virus: Pectobacterium phage phiTE (virus)
    • Protein or peptide: x 18 types
KeywordsTail / Sheath / Tube / PhiTE / Virus / Phage
Function / homology
Function and homology information


virus tail, fiber / viral tail assembly / host cell cytoplasm / virion attachment to host cell
Similarity search - Function
: / : / Dit-like phage tail protein / Cyanophage baseplate Pam3 plug gp18 / Phage neck terminator protein / Tape measure protein N-terminal / Protein of unknown function DUF2612 / Protein of unknown function (DUF2612) / Tape measure protein / Phage tail collar domain ...: / : / Dit-like phage tail protein / Cyanophage baseplate Pam3 plug gp18 / Phage neck terminator protein / Tape measure protein N-terminal / Protein of unknown function DUF2612 / Protein of unknown function (DUF2612) / Tape measure protein / Phage tail collar domain / Phage tail collar domain superfamily / Phage protein Gp138 N-terminal domain / Phage Tail Collar Domain / Phage protein Gp138 N-terminal domain / Structural protein ORF10, bacteriophage KPP10 / Bacteriophage KPP10, Structural protein ORF10 / : / Bacteriophage SPP1, head-tail adaptor / Phage head-tail joining protein / Major capsid protein GpE / Phage major capsid protein E / Vgr protein, OB-fold domain superfamily / Bacterial Ig-like domain (group 2) / Invasin/intimin cell-adhesion fragments / Bacterial Ig-like domain, group 2
Similarity search - Domain/homology
Putative major head protein / Uncharacterized protein / Structural protein / Putative tape measure protein / Uncharacterized protein / Head stabilization/decoration protein / Structural protein / Putative baseplate assembly protein / Uncharacterized protein / Cyanophage baseplate Pam3 plug gp18 domain-containing protein ...Putative major head protein / Uncharacterized protein / Structural protein / Putative tape measure protein / Uncharacterized protein / Head stabilization/decoration protein / Structural protein / Putative baseplate assembly protein / Uncharacterized protein / Cyanophage baseplate Pam3 plug gp18 domain-containing protein / Uncharacterized protein / Portal protein / Dit-like phage tail protein N-terminal domain-containing protein / Putative baseplate assembly protein / Uncharacterized protein / Tail protein / Uncharacterized protein / Putative tail fiber protein
Similarity search - Component
Biological speciesPectobacterium phage phiTE (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 12.7 Å
AuthorsHodgkinson-Bean J / Ayala R
Funding support Japan, 2 items
OrganizationGrant numberCountry
Ministry of Agriculture, Forestry and Fisheries (MAFF)GD0696J004 Japan
Japan Society for the Promotion of Science (JSPS)21K20645 Japan
CitationJournal: Nat Commun / Year: 2025
Title: Global structural survey of the flagellotropic myophage φTE infecting agricultural pathogen Pectobacterium atrosepticum.
Authors: James Hodgkinson-Bean / Rafael Ayala / Nadishka Jayawardena / Georgia L Rutter / Bridget N J Watson / David Mayo-Muñoz / James Keal / Peter C Fineran / Matthias Wolf / Mihnea Bostina /
Abstract: Bacteriophages offer a promising alternative to drug-based treatments due to their effectiveness and host specificity. This is particularly important in agriculture as a biocontrol agent of plant ...Bacteriophages offer a promising alternative to drug-based treatments due to their effectiveness and host specificity. This is particularly important in agriculture as a biocontrol agent of plant diseases. Phage engineering is facilitated by structural knowledge. However, structural information regarding bacteriophages infecting plant pathogens is limited. Here, we present the cryo-EM structure of bacteriophage φTE that infects plant pathogen Pectobacterium atrosepticum. The structure reveals a distinct neck topology compared with other myophages, where tail terminator proteins compensate for reduced connectivity between sheath subunits. A contact network between tail fibers, the sheath initiator, and baseplate wedge proteins provides insights into triggers that transduce conformational changes from the baseplate to the sheath to orchestrate contraction. We observe two distinct oligomeric states of the tape measure protein (TMP), which is six-fold in regions proximal to the N-terminus and throughout most of the tail, while three-fold at the C-terminus, indicating that the TMP may be proteolytically cleaved. Our results provide a structural atlas of the model bacteriophage φTE, enhancing future interpretation of phage host interactions in pectobacteria. We anticipate that our structure will inform rational design of biocontrol agents against plant pathogens that cause diseases such as soft rot and blackleg disease in potatoes.
History
DepositionDec 16, 2024-
Header (metadata) releaseApr 16, 2025-
Map releaseApr 16, 2025-
UpdateApr 16, 2025-
Current statusApr 16, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48317.png

Downloads & links

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Map

FileDownload / File: emd_48317.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
6.3 Å/pix.
x 440 pix.
= 2773.98 Å		6.3 Å/pix.
x 440 pix.
= 2773.98 Å		6.3 Å/pix.
x 440 pix.
= 2773.98 Å

Surface

Projections

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Images are generated by Spider.

Voxel sizeX=Y=Z: 6.3045 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0356
Minimum - Maximum-0.40040225 - 0.4595237
Average (Standard dev.)0.0004260768 (±0.012086198)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 2773.98 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_48317_additional_1.map
Projections & Slices
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Half map: #1

Fileemd_48317_half_map_1.map
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Half map: #2

Fileemd_48317_half_map_2.map
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Sample components

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Entire : Pectobacterium phage phiTE

EntireName: Pectobacterium phage phiTE (virus)
Components
  • Virus: Pectobacterium phage phiTE (virus)
    • Protein or peptide: Portal protein
    • Protein or peptide: PhiTE adaptor protein
    • Protein or peptide: PhiTE head completion protein
    • Protein or peptide: PhiTE tail terminator protein
    • Protein or peptide: Structural protein
    • Protein or peptide: Tail sheath protein
    • Protein or peptide: Head stabilization/decoration protein
    • Protein or peptide: Major capsid protein
    • Protein or peptide: Putative tail fiber protein
    • Protein or peptide: Sheath initiator protein
    • Protein or peptide: Dit-like phage tail protein N-terminal domain-containing protein
    • Protein or peptide: Cyanophage baseplate Pam3 plug gp18 domain-containing protein
    • Protein or peptide: Wedge 1 protein
    • Protein or peptide: baseplate assembly protein
    • Protein or peptide: Putative tape measure protein
    • Protein or peptide: Tail protein
    • Protein or peptide: Putative baseplate assembly protein
    • Protein or peptide: Short tail fiber

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Supramolecule #1: Pectobacterium phage phiTE

SupramoleculeName: Pectobacterium phage phiTE / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 1116482 / Sci species name: Pectobacterium phage phiTE / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Pectobacterium atrosepticum (bacteria)
Virus shellShell ID: 1 / Diameter: 1000.0 Å / T number (triangulation number): 13

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Macromolecule #1: Portal protein

MacromoleculeName: Portal protein / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 55.500016 KDa
SequenceString: MSRKRNRNRS VQVAKATSEQ LNVSRMRMSE QGTFALAKVQ VDSERMKAEE IRWPHLIGTA ESMKQDATVA TGLDMLYTFV EKAFKDFKV IPGESEESKK AAKFIEYCLK NMEGQTLRQF ARDAATFNEY GLSVVEKVYT QIAVGEYVGK YKVKNLAFRP Q ASLSRTNP ...String:
MSRKRNRNRS VQVAKATSEQ LNVSRMRMSE QGTFALAKVQ VDSERMKAEE IRWPHLIGTA ESMKQDATVA TGLDMLYTFV EKAFKDFKV IPGESEESKK AAKFIEYCLK NMEGQTLRQF ARDAATFNEY GLSVVEKVYT QIAVGEYVGK YKVKNLAFRP Q ASLSRTNP IVYNSDGSAI VGIKQSLSAF QNYTASEIGV GGVSTRMSDV IIPISRVMLM NTGGSSSQAL GVSPLVGCYR AW REKILIE NLEVVGATKD MGGVIELKIP SQILNKAAMD PSSPEADMVR GLMSDAANAH SGEQSFFMLP SDTKDNAPQY SMT LKGIDG MGKQYSTAQL ISDRKKSILD RLGAGFINVG NDKGGSYNLS ESKQTIHTQF VQRVNEIILE ALNENLLPQL LALN DIRLP ETEMPYVKAG EIVDVDMEGF SKAIQRIGAV GYLPKTPKVI NRVLEVLGID EKIEEDISQE ELMKLLGEDT SRAGD GMTK GSSGNGTGKI SASRDNSAAN LDN

UniProtKB: Portal protein

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Macromolecule #2: PhiTE adaptor protein

MacromoleculeName: PhiTE adaptor protein / type: protein_or_peptide / ID: 2 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 20.13277 KDa
SequenceString:
MTNEQVIELV RVLLGGITTE EISDQTIIFF WTKWKLTYDL DNRPEKIPAA LYNTVVDCVR WLIVQEVSSG NSSIRERFEK IGDETISVK GGSSWESWKD FLDWLELNPD YIDPSLAFNS SLVIIGGVRK DEFFRVKNNP NSYNGFMEQG VYPTPAIPKQ S AWPCTAAR RSPWMVR

UniProtKB: Uncharacterized protein

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Macromolecule #3: PhiTE head completion protein

MacromoleculeName: PhiTE head completion protein / type: protein_or_peptide / ID: 3 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 16.58576 KDa
SequenceString:
MARAYSLLSS RNRLIPRVEV QCRKREWVKT DPDSPFLNGG REVLYTPFTA VECTVQPMRG KAIRDQNNQL MIGGEEDYDS YTVYSETLL FRAREGTEHL SDQMLLPDSG GGQTWFTVMK ADMYPSSGVP RYRYYLIAVP VGTEGGL

UniProtKB: Uncharacterized protein

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Macromolecule #4: PhiTE tail terminator protein

MacromoleculeName: PhiTE tail terminator protein / type: protein_or_peptide / ID: 4 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 25.622951 KDa
SequenceString: MALPLDFTNS DVVMGALTKA VGRLCLDVTG YDVVEADETI PKPEGPYILV DLSLLTPLDW ATNEVVDEDG VVHTAHNYTA SYTLTAYRG KPHWALSRVH QAFGLPFLRE KYFPTGSPYA YSSTSNIARM RVPLNQQMFE NRARTIVTFN ATFVEKDLGT F EDIEHIII ...String:
MALPLDFTNS DVVMGALTKA VGRLCLDVTG YDVVEADETI PKPEGPYILV DLSLLTPLDW ATNEVVDEDG VVHTAHNYTA SYTLTAYRG KPHWALSRVH QAFGLPFLRE KYFPTGSPYA YSSTSNIARM RVPLNQQMFE NRARTIVTFN ATFVEKDLGT F EDIEHIII GIDVDNPSGP PIGIGADYDK GVKPGGDDPG LPPKPNPPIV YHDAIAQVCM ATPVIDKPAL ISDKTGE

UniProtKB: Uncharacterized protein

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Macromolecule #5: Structural protein

MacromoleculeName: Structural protein / type: protein_or_peptide / ID: 5 / Number of copies: 162 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 17.28042 KDa
SequenceString:
MLNQSKILTL QAYDPAKVLV FIGGQRVSGF AADTKIVITR NNDNISVHAG VDGEISNALS RDNTGVMTLS LQNTAKWNGY LAQWQRQAN VTGLIYLPVQ VEGSQGLSLN TIGWIQKQPD LSYGTEVGQM DWEIGVLDAW LSPDQIQGIA AGITGLLGLD Q

UniProtKB: Structural protein

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Macromolecule #6: Tail sheath protein

MacromoleculeName: Tail sheath protein / type: protein_or_peptide / ID: 6 / Number of copies: 162 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 50.647766 KDa
SequenceString: MAEYQDKVVD VEVSLGTQPI DTVGFETPMF LAMHGNFPER IRFYVSTAGM VADGFAVGSP AYQFATNAFA GNFAPQRVAI GRMSIDSSK VDFTGTTNTE QVVVNITLNK VVKAVKINVL PGNTPAQIAT ALADAVTADA DLTGKATAVA TGTYVTVTAV S PNVVSVGK ...String:
MAEYQDKVVD VEVSLGTQPI DTVGFETPMF LAMHGNFPER IRFYVSTAGM VADGFAVGSP AYQFATNAFA GNFAPQRVAI GRMSIDSSK VDFTGTTNTE QVVVNITLNK VVKAVKINVL PGNTPAQIAT ALADAVTADA DLTGKATAVA TGTYVTVTAV S PNVVSVGK GAGVYKIVNE SSETVATVLP SVIAENHNWY FLATEARSDA DIVAAAEFAK ANYKLHIYNS TDVDAYAPEN SA ASVFDTL KSLSYDSLGT SDAGADVDFT EGSVIGAMAA NDPSYGDSLH LKTMPGMVPF AGSDTQRSNA WSRNANIYRG LYG GGSYIE GKTSSGQYVD VIRFSHWVKF RMEESVFAYM KRRSDMGLSM KMSDEDLPVL KSVLMNNPIN IGIRNGGILT GYDT ENKVS YDPTIIIPKR ANIPTNDLAA RILRDVKVEL VYNNSLHYVK IRASVVLDRP AGQSTNAQTP MSSSAVGV

UniProtKB: Structural protein

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Macromolecule #7: Head stabilization/decoration protein

MacromoleculeName: Head stabilization/decoration protein / type: protein_or_peptide / ID: 7 / Number of copies: 780 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 15.552637 KDa
SequenceString:
MAKAHVATLE GNYSDIVLGR VVAFGDTGWN FKEVDMTFIA DDADADSKTT LFAGVLVGED GTPATAAAGV FGVLVDRKVL PGVDHYIGV FEPGEKVPMV LAVRGLTLNQ LKLKYADGTA IDAAGIQALE AQGNQVTDKI VGTQFIGSVL

UniProtKB: Head stabilization/decoration protein

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Macromolecule #8: Major capsid protein

MacromoleculeName: Major capsid protein / type: protein_or_peptide / ID: 8 / Number of copies: 775 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 37.596047 KDa
SequenceString: MQNGDFQILD YTGLISTMPR VDTLLQSMNL FTEHFGRTTV ARIERLDDGA GDIKAVQRGG VRQHLANDRK KIVNLNIPFF PLDRSIDRA DIQNFREFGT ENAPATVDAE VQRHMARIRR SHAILKSKAM YAALKGTSWS PDDPVSDYNY YDVWGATQTT A DVDFTKLG ...String:
MQNGDFQILD YTGLISTMPR VDTLLQSMNL FTEHFGRTTV ARIERLDDGA GDIKAVQRGG VRQHLANDRK KIVNLNIPFF PLDRSIDRA DIQNFREFGT ENAPATVDAE VQRHMARIRR SHAILKSKAM YAALKGTSWS PDDPVSDYNY YDVWGATQTT A DVDFTKLG VDPIEVLEAE ARAHIIDWAG DNGDNYEIVV LASRQWFSAL IAHPQVTGAY SQYPSTQEIL RRRLGGNANN RI FEHKNIL FIEDISGNIP AGEAYIFPRG ISRMFEIYYA PSDTLRDANQ AAQELYVFFK ESNYLREAKI ESETSFLTVN NRP ELVVKS TGKFTA

UniProtKB: Putative major head protein

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Macromolecule #9: Putative tail fiber protein

MacromoleculeName: Putative tail fiber protein / type: protein_or_peptide / ID: 9 / Number of copies: 18 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 81.935617 KDa
SequenceString: MSSNTDIIAL LSSDADKAVD QIIEDSKRLH IVVNGTGTEQ AVAEDGSLLP SVRKALIDNL YFKTPPLPWR NGGSVNEFNQ LYSFTDVSG NTTWWYAPGA TVSNPVVMRD SPINDGKFKV FLDKTNIADI YAPLNSPNFV GNPRVPTPAP GDNSQSIPTT G WVQSEMEG ...String:
MSSNTDIIAL LSSDADKAVD QIIEDSKRLH IVVNGTGTEQ AVAEDGSLLP SVRKALIDNL YFKTPPLPWR NGGSVNEFNQ LYSFTDVSG NTTWWYAPGA TVSNPVVMRD SPINDGKFKV FLDKTNIADI YAPLNSPNFV GNPRVPTPAP GDNSQSIPTT G WVQSEMEG LEDLIISSMR GEFENITVRN DAILQDTYIN GEFIVTSDKL AAANTEATFR RIRMVGTGTT GTAATEIAFE QN TTPPPGV STKTNIKPFA VSTGILTVDQ INGKAAQVGD VAVSTDSLNV DGYMRGDYLH LEGNNRNTAG RPQLIVDGIA EIE TLRVTG TIEGVKADVD GLDIHPRSVI VDEGLTVAKD TTMTGKLTVG GTTKVGNLEV TGTLSGVAIS VDGKDIKPRS VNTG NLVAT GDVQISGTTT ATGKITTQDL EVLGTLTANL DLSGSDLSVD NINVAQTATI TDLVVTGTTT GVKGDVEGEA IAPAS VAAT GTVSAASMST TGNMAVGGNL TITGDFRPAS VAGQDTPRLV VQGDSDLQGD LNVSGTITGV VDLTAQDINM NSLGLA QGL TAGGGITSFS TVKGVNGVFG EGSTAPGAIG LQSMTNAKVA TDLLVGEDLR VEGDTNLFGN LSVTGTITGN MNFSGKT IA PATVNATTVT ATNLTVTGTI DLTDSPIVAQ EITATNVEAT GKMVAGKYST TPKTANATTA TFTPDGTTSI YNVNVQAD T EVQAPAGLLS SGKGESIFIY FEQDTTGHAV TFSSAFVVHN AATVSNAANS ITIANLVYRG TGPLIDVFLT GR

UniProtKB: Putative tail fiber protein

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Macromolecule #10: Sheath initiator protein

MacromoleculeName: Sheath initiator protein / type: protein_or_peptide / ID: 10 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 20.452148 KDa
SequenceString:
MATQFKDLLL DPLTGDLDFG TPGDRGMRLA LTNQLSLRQR LYLRFAIWAG DWYFDETFGF PYRTFVGKKT VKAVLDGRIK SEVRQEPDV LQITDFQSTM DVVSRSYKCF FTVVTAEGEE ISLAFVGEDE YQYPTPPESN VQLCGDEGVI INFKNKLYYL I NFRLPKYG DSTWVNSWK

UniProtKB: Uncharacterized protein

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Macromolecule #11: Dit-like phage tail protein N-terminal domain-containing protein

MacromoleculeName: Dit-like phage tail protein N-terminal domain-containing protein
type: protein_or_peptide / ID: 11 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 31.720324 KDa
SequenceString: MAVNNNSISP AQQAQADKRA RDAAAAKRKQ DTEVSNAKSR EDIQYTMFVS GLRRGRLNEF ERKNRTDDLA ILFDSVTTHT YTKDYNKSS YAVESKAKAS DHVTTQDGKF TFSGTVTDSP YLIDPRNMID RDTDKENPML ARRPAKAIEI LELIADSHQL V TLVTEDNI ...String:
MAVNNNSISP AQQAQADKRA RDAAAAKRKQ DTEVSNAKSR EDIQYTMFVS GLRRGRLNEF ERKNRTDDLA ILFDSVTTHT YTKDYNKSS YAVESKAKAS DHVTTQDGKF TFSGTVTDSP YLIDPRNMID RDTDKENPML ARRPAKAIEI LELIADSHQL V TLVTEDNI LSNYVITSFQ VDRSSEAGSS INVQVTLEEF RFKMVNKTVM ARTSDPKKAK NANTGTKQTA EDGAVDDSAK QK RQTPYIG KNAETKERWE NAAIGTTDFS GKPGAKLPFD PSDLMRK

UniProtKB: Dit-like phage tail protein N-terminal domain-containing protein

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Macromolecule #12: Cyanophage baseplate Pam3 plug gp18 domain-containing protein

MacromoleculeName: Cyanophage baseplate Pam3 plug gp18 domain-containing protein
type: protein_or_peptide / ID: 12 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 14.404139 KDa
SequenceString:
MANTTERTVV RTFDFELSGY PDETFRVVLD SVTYELRFMW NERDESWFMS LGDIGAQRPT ITSKLTCYSD ILAPYRYLDN VPDGNLYLW PLGDIRTRAG RFNIGPLKGI QMTYSSLIED VEDIE

UniProtKB: Cyanophage baseplate Pam3 plug gp18 domain-containing protein

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Macromolecule #13: Wedge 1 protein

MacromoleculeName: Wedge 1 protein / type: protein_or_peptide / ID: 13 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 24.21515 KDa
SequenceString: MVEEIVLKDV NHIHPLPDFV RGGFDYVPSD IFSGKENFEQ TLRILLERLE FIDQKMVELA ELRTTLNAED AILDEIGRQL GIYRNGLND PEYRAVIMIL TGNNSKSGTR ADIIATLKQL FGEDGVTTYK GYNYRLDINI FNTCMEVTDI LPEIIDMLPL V THLRVVEN ...String:
MVEEIVLKDV NHIHPLPDFV RGGFDYVPSD IFSGKENFEQ TLRILLERLE FIDQKMVELA ELRTTLNAED AILDEIGRQL GIYRNGLND PEYRAVIMIL TGNNSKSGTR ADIIATLKQL FGEDGVTTYK GYNYRLDINI FNTCMEVTDI LPEIIDMLPL V THLRVVEN QGYPFGFNGD TQAFGFASVY DSGRTGEGGM STLIYVSDDE EDWSFT

UniProtKB: Uncharacterized protein

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Macromolecule #14: baseplate assembly protein

MacromoleculeName: baseplate assembly protein / type: protein_or_peptide / ID: 14 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 54.432738 KDa
SequenceString: MAENYGLTGS GFNLPPMDDL VQETKKTFKS AFGEDFNTES NSVADKLIQI FNEREYQLWL LMGSVYYAQT MQGAEGIYLD DLLGKRGIY RLGKTRSTGT VVMTIDSSVP YNMIYSAATY TIDTDYELSS DVQVAGNIVA QLIKGTDLSV GTYRLQIQNT T DQSVKTLS ...String:
MAENYGLTGS GFNLPPMDDL VQETKKTFKS AFGEDFNTES NSVADKLIQI FNEREYQLWL LMGSVYYAQT MQGAEGIYLD DLLGKRGIY RLGKTRSTGT VVMTIDSSVP YNMIYSAATY TIDTDYELSS DVQVAGNIVA QLIKGTDLSV GTYRLQIQNT T DQSVKTLS LNLTATSGQP LITFFGQIKD FIVNNTILSN QDRIWIDSTE GALYIGYDTN KIMIGLSSRV DFRTNPMAGT RS ISMDVRS IEPGYISRDV HSVRSINPTP GGFVDIDNLS AFIDGSDVES DNEYRIRAAT SISEGKATRP AILAALLNKV EGI EKVRIF NNNTDKTNSL GIPPYRFMVV CYGGGTAEIS QVLYDTIATS NNTYGDTFYD ITTEDDQVER IWHTKAAARQ LAIR VRYRG RPLSLTEETA IANGLATAVN GTMIAGTLYN VRLVGTVMSS TSPDRFTQVY VDIKNKGQPD SAYVNTDVTA STTQV LSLE LEDVIFSQIV

UniProtKB: Putative baseplate assembly protein

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Macromolecule #15: Putative tape measure protein

MacromoleculeName: Putative tape measure protein / type: protein_or_peptide / ID: 15 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 87.898227 KDa
SequenceString: MAASGNVVVT RTTNRVTFET DKASYARAVK QIRQVGKEWE KASDAISKPK KDPAKAYDKS AQQMRLVNKR LAETRAREEK RASERSIAL ARREAKAKEA IARVSAARIK QQVQQMTSPR AGMSEMKKFY QQQEREAKKA NRKATIGMNA ASRPMNVTRI N SAPLSPRG ...String:
MAASGNVVVT RTTNRVTFET DKASYARAVK QIRQVGKEWE KASDAISKPK KDPAKAYDKS AQQMRLVNKR LAETRAREEK RASERSIAL ARREAKAKEA IARVSAARIK QQVQQMTSPR AGMSEMKKFY QQQEREAKKA NRKATIGMNA ASRPMNVTRI N SAPLSPRG GQGTGMVGDP NKVYNPELIA AQNRAMAGRI RSQSQAQDRK AKAEARQRAA AAARAARIDD VMAQQRIRLS SK YGRSYEG RLGRDGAGQG IQDLNRQFRA GTLSAGQYRQ SIQALERQFR SAQANAGGFG AALGDIRSQL LNAGAAYGVF ASG ASVLKQ GQFFQGMEAT MSMVSDSSEE AGQRIKFVKD QAYRLGLDLK IASQGYTQMA VNSAGILSKA QNDDLFKGFS EFAT ASQVD PVKFQRGITA IGQMMGKGQV MAEELKGQLA EGIPGSLQVF VKAAQEAFGD TTIDVEKLMD MMQKGELKAA KILPF VGKY FAEAARKGGA LPKALESNRV AMQRLSLTWI DFQNQIFQGG FGEQMTRVFR DLATILDSNG ELATNLGAFF GNVIEG FWD MVTEIHDDFV LLDRIVSYYT EKLGYQGDLL KEVFDWAGYA IGIGIFVGGL NKIFKILTKI AGLRTALVGV RQAMGGA IT RGGVTNGAGL GGGAGAAGTP GASRTASFLS KWKGLSKISK VGILGTLYSA GSMLNEQFIK RGDDKLADAG LDQETFQK E YGFMPKPVGL LDVFDKWFNK PRDLDAPTIT APGPGSGMAA QTGIPFPAPQ KVEGDITIKI EAGELRNMVR AVVDENNQF NFNMLIQGGP N

UniProtKB: Putative tape measure protein

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Macromolecule #16: Tail protein

MacromoleculeName: Tail protein / type: protein_or_peptide / ID: 16 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 36.545242 KDa
SequenceString: MRAMERTYTL VIGRPVVIGE KPVNIEKFAN TSKGDAYEIK DLHIEFNVKK DNSKEPNKGY VTVYNLSDEV VNYLSVNQRE SLAVMLHAG YNGDEKLIFS GTVEYVEDDF PEETRTTKFI LGDGTLNLTT ATTARSYRKG TPVNSVLNDL IADLKLPKGR V IDFGNQTL ...String:
MRAMERTYTL VIGRPVVIGE KPVNIEKFAN TSKGDAYEIK DLHIEFNVKK DNSKEPNKGY VTVYNLSDEV VNYLSVNQRE SLAVMLHAG YNGDEKLIFS GTVEYVEDDF PEETRTTKFI LGDGTLNLTT ATTARSYRKG TPVNSVLNDL IADLKLPKGR V IDFGNQTL QTSMAFTGNA SQNLANLAKN TGSTFSVQDG AVYWTKEGSR FNNVMFEISE EGGMVGTPTP KQPSSSKKLI KA KAKAKSD EAEGKKPKAP SKKKKEHDIK EDVGMTVSTL LNGAILPEST VYLNTRYHKG FYKVAELTHR GGYETGDWIT ELG LVETRG ELIK

UniProtKB: Tail protein

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Macromolecule #17: Putative baseplate assembly protein

MacromoleculeName: Putative baseplate assembly protein / type: protein_or_peptide / ID: 17 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 26.474344 KDa
SequenceString: MAYADRYDAA FGIYISRFLR NNVHTNIRGK VVGVNYSGPS VDVQPMAYTE FPSGTTDRYP VIYDVPVLLP SGAGGKARLT MPIKPGDVV GLSFSERNEG DNNDQNTHQL FAGWAVTQIF TDGNSKAIHP DNVVLENDKA IITLKPDGDT SLQNPKVTVE A LADGNVKI ...String:
MAYADRYDAA FGIYISRFLR NNVHTNIRGK VVGVNYSGPS VDVQPMAYTE FPSGTTDRYP VIYDVPVLLP SGAGGKARLT MPIKPGDVV GLSFSERNEG DNNDQNTHQL FAGWAVTQIF TDGNSKAIHP DNVVLENDKA IITLKPDGDT SLQNPKVTVE A LADGNVKI SNGTGEFTMS PSGEIKGGNR GGSFTLQPSG QLMCNGARIT TSGRIITANG VDMDDFYQKY MSHTHGGVSR GG DNTNPPN

UniProtKB: Putative baseplate assembly protein

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Macromolecule #18: Short tail fiber

MacromoleculeName: Short tail fiber / type: protein_or_peptide / ID: 18 / Number of copies: 18 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 59.236 KDa
SequenceString: MAKPKDPIYV WAAADVNLPG TGRPNKSKPI DDLLAKGYDK GQKPAAEEFN YILNMSSAWV NWIVNEKFPE LEAEIARLLA ELENRINQQ LAVIRQDIAQ LRQDVADLRR YVDQKVQELK QEIQGVRNDL NKLRQDFDAA ITQVNGRIDD LEPRLVPIGA V IPWPGATV ...String:
MAKPKDPIYV WAAADVNLPG TGRPNKSKPI DDLLAKGYDK GQKPAAEEFN YILNMSSAWV NWIVNEKFPE LEAEIARLLA ELENRINQQ LAVIRQDIAQ LRQDVADLRR YVDQKVQELK QEIQGVRNDL NKLRQDFDAA ITQVNGRIDD LEPRLVPIGA V IPWPGATV PDGWLECSGQ VFNTGQNPKL YSVLGRNVVP DYRGLFLRGW AHGSDANDPD AGRALGSVQG DAIRNITGYF PA DIGNSNF IGRYVGGAFR DDGSLTSGDD GTRSNEVRKY SFDASREVPV AAENRPKNIA VMYIIKTDQA QSSGGNSPTA IVV SPDTIT NRIGYAVKAT ASVLPASISG QYPISWSTQN AAVATVDGSG NISLVGPGET NIIASISTGM NVVIRVTSYS VLTS ISIAD PGQITVTESK MLVVTKSPSS ANEPLQFLSN NSGVAAVNDA GYVIGVSAGS ATITVRGTLS GVSSTRAVTI IPEQV EESV QDNRLGAVGS YIPPSPSSNV MSWTFQAPQG CALTGIIVQE NESNSGDNIG GVYYKPIQKK VNGVWVTITG

UniProtKB: Uncharacterized protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 4 K

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: OTHER / Details: Ab initio helical model generated in cryoSPARC
Final reconstructionResolution.type: BY AUTHOR / Resolution: 12.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12638
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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