: / : / Dit-like phage tail protein / Cyanophage baseplate Pam3 plug gp18 / Tape measure protein N-terminal / Protein of unknown function DUF2612 / Protein of unknown function (DUF2612) / Tape measure protein / Phage tail collar domain / Phage tail collar domain superfamily ...: / : / Dit-like phage tail protein / Cyanophage baseplate Pam3 plug gp18 / Tape measure protein N-terminal / Protein of unknown function DUF2612 / Protein of unknown function (DUF2612) / Tape measure protein / Phage tail collar domain / Phage tail collar domain superfamily / Phage protein Gp138 N-terminal domain / Phage Tail Collar Domain / Phage protein Gp138 N-terminal domain / Structural protein ORF10, bacteriophage KPP10 / Bacteriophage KPP10, Structural protein ORF10 / : / Vgr protein, OB-fold domain superfamily / Bacterial Ig-like domain (group 2) / Invasin/intimin cell-adhesion fragments / Bacterial Ig-like domain, group 2 類似検索 - ドメイン・相同性
Structural protein / Putative tape measure protein / Uncharacterized protein / Structural protein / Putative baseplate assembly protein / Cyanophage baseplate Pam3 plug gp18 domain-containing protein / Uncharacterized protein / Dit-like phage tail protein N-terminal domain-containing protein / Putative baseplate assembly protein / Tail protein ...Structural protein / Putative tape measure protein / Uncharacterized protein / Structural protein / Putative baseplate assembly protein / Cyanophage baseplate Pam3 plug gp18 domain-containing protein / Uncharacterized protein / Dit-like phage tail protein N-terminal domain-containing protein / Putative baseplate assembly protein / Tail protein / Uncharacterized protein / Putative tail fiber protein 類似検索 - 構成要素
Ministry of Agriculture, Forestry and Fisheries (MAFF)
GD0696J004
日本
Japan Society for the Promotion of Science (JSPS)
21K20645
日本
引用
ジャーナル: Nat Commun / 年: 2025 タイトル: Global structural survey of the flagellotropic myophage φTE infecting agricultural pathogen Pectobacterium atrosepticum. 著者: James Hodgkinson-Bean / Rafael Ayala / Nadishka Jayawardena / Georgia L Rutter / Bridget N J Watson / David Mayo-Muñoz / James Keal / Peter C Fineran / Matthias Wolf / Mihnea Bostina / 要旨: Bacteriophages offer a promising alternative to drug-based treatments due to their effectiveness and host specificity. This is particularly important in agriculture as a biocontrol agent of plant ...Bacteriophages offer a promising alternative to drug-based treatments due to their effectiveness and host specificity. This is particularly important in agriculture as a biocontrol agent of plant diseases. Phage engineering is facilitated by structural knowledge. However, structural information regarding bacteriophages infecting plant pathogens is limited. Here, we present the cryo-EM structure of bacteriophage φTE that infects plant pathogen Pectobacterium atrosepticum. The structure reveals a distinct neck topology compared with other myophages, where tail terminator proteins compensate for reduced connectivity between sheath subunits. A contact network between tail fibers, the sheath initiator, and baseplate wedge proteins provides insights into triggers that transduce conformational changes from the baseplate to the sheath to orchestrate contraction. We observe two distinct oligomeric states of the tape measure protein (TMP), which is six-fold in regions proximal to the N-terminus and throughout most of the tail, while three-fold at the C-terminus, indicating that the TMP may be proteolytically cleaved. Our results provide a structural atlas of the model bacteriophage φTE, enhancing future interpretation of phage host interactions in pectobacteria. We anticipate that our structure will inform rational design of biocontrol agents against plant pathogens that cause diseases such as soft rot and blackleg disease in potatoes.