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- EMDB-45953: Bacteriophage PhiTE extended baseplate -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-45953
TitleBacteriophage PhiTE extended baseplate
Map data
Sample
  • Virus: Pectobacterium phage phiTE (virus)
    • Protein or peptide: x 12 types
Keywordsbaseplate / Sheath / Tube / PhiTE / Virus / Phage / VIRAL PROTEIN / sheath initiator / tube initiator / hub / puncture apparatus / fibers / wedge / tape measure protein
Function / homology
Function and homology information


virus tail, fiber / viral tail assembly / virion attachment to host cell
Similarity search - Function
: / : / Dit-like phage tail protein / Cyanophage baseplate Pam3 plug gp18 / Tape measure protein N-terminal / Protein of unknown function DUF2612 / Protein of unknown function (DUF2612) / Tape measure protein / Phage tail collar domain / Phage tail collar domain superfamily ...: / : / Dit-like phage tail protein / Cyanophage baseplate Pam3 plug gp18 / Tape measure protein N-terminal / Protein of unknown function DUF2612 / Protein of unknown function (DUF2612) / Tape measure protein / Phage tail collar domain / Phage tail collar domain superfamily / Phage protein Gp138 N-terminal domain / Phage Tail Collar Domain / Phage protein Gp138 N-terminal domain / Structural protein ORF10, bacteriophage KPP10 / Bacteriophage KPP10, Structural protein ORF10 / : / Vgr protein, OB-fold domain superfamily / Bacterial Ig-like domain (group 2) / Invasin/intimin cell-adhesion fragments / Bacterial Ig-like domain, group 2
Similarity search - Domain/homology
Structural protein / Putative tape measure protein / Uncharacterized protein / Structural protein / Putative baseplate assembly protein / Cyanophage baseplate Pam3 plug gp18 domain-containing protein / Uncharacterized protein / Dit-like phage tail protein N-terminal domain-containing protein / Putative baseplate assembly protein / Tail protein ...Structural protein / Putative tape measure protein / Uncharacterized protein / Structural protein / Putative baseplate assembly protein / Cyanophage baseplate Pam3 plug gp18 domain-containing protein / Uncharacterized protein / Dit-like phage tail protein N-terminal domain-containing protein / Putative baseplate assembly protein / Tail protein / Uncharacterized protein / Putative tail fiber protein
Similarity search - Component
Biological speciesPectobacterium phage phiTE (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.24 Å
AuthorsHodgkinson-Bean J / Ayala R
Funding support Japan, 2 items
OrganizationGrant numberCountry
Ministry of Agriculture, Forestry and Fisheries (MAFF)GD0696J004 Japan
Japan Society for the Promotion of Science (JSPS)21K20645 Japan
CitationJournal: Nat Commun / Year: 2025
Title: Global structural survey of the flagellotropic myophage φTE infecting agricultural pathogen Pectobacterium atrosepticum.
Authors: James Hodgkinson-Bean / Rafael Ayala / Nadishka Jayawardena / Georgia L Rutter / Bridget N J Watson / David Mayo-Muñoz / James Keal / Peter C Fineran / Matthias Wolf / Mihnea Bostina /
Abstract: Bacteriophages offer a promising alternative to drug-based treatments due to their effectiveness and host specificity. This is particularly important in agriculture as a biocontrol agent of plant ...Bacteriophages offer a promising alternative to drug-based treatments due to their effectiveness and host specificity. This is particularly important in agriculture as a biocontrol agent of plant diseases. Phage engineering is facilitated by structural knowledge. However, structural information regarding bacteriophages infecting plant pathogens is limited. Here, we present the cryo-EM structure of bacteriophage φTE that infects plant pathogen Pectobacterium atrosepticum. The structure reveals a distinct neck topology compared with other myophages, where tail terminator proteins compensate for reduced connectivity between sheath subunits. A contact network between tail fibers, the sheath initiator, and baseplate wedge proteins provides insights into triggers that transduce conformational changes from the baseplate to the sheath to orchestrate contraction. We observe two distinct oligomeric states of the tape measure protein (TMP), which is six-fold in regions proximal to the N-terminus and throughout most of the tail, while three-fold at the C-terminus, indicating that the TMP may be proteolytically cleaved. Our results provide a structural atlas of the model bacteriophage φTE, enhancing future interpretation of phage host interactions in pectobacteria. We anticipate that our structure will inform rational design of biocontrol agents against plant pathogens that cause diseases such as soft rot and blackleg disease in potatoes.
History
DepositionJul 26, 2024-
Header (metadata) releaseApr 16, 2025-
Map releaseApr 16, 2025-
UpdateApr 16, 2025-
Current statusApr 16, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_45953.png

Downloads & links

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Map

FileDownload / File: emd_45953.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
1.39 Å/pix.
x 512 pix.
= 710.144 Å		1.39 Å/pix.
x 512 pix.
= 710.144 Å		1.39 Å/pix.
x 512 pix.
= 710.144 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.387 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 6.5
Minimum - Maximum-10.402727000000001 - 22.643469
Average (Standard dev.)0.015242917 (±1.080392)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 710.144 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_45953_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_45953_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Pectobacterium phage phiTE

EntireName: Pectobacterium phage phiTE (virus)
Components
  • Virus: Pectobacterium phage phiTE (virus)
    • Protein or peptide: Wedge 2 protein
    • Protein or peptide: Tail sheath protein
    • Protein or peptide: Tail tube protein
    • Protein or peptide: Tube initiator protein
    • Protein or peptide: Hub protein
    • Protein or peptide: Putative baseplate assembly protein
    • Protein or peptide: Wedge 1 protein
    • Protein or peptide: Spacer protein
    • Protein or peptide: Sheath initiator protein
    • Protein or peptide: Putative tail fiber protein
    • Protein or peptide: Short tail fiber
    • Protein or peptide: Putative tape measure protein

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Supramolecule #1: Pectobacterium phage phiTE

SupramoleculeName: Pectobacterium phage phiTE / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 1116482 / Sci species name: Pectobacterium phage phiTE / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Pectobacterium atrosepticum (bacteria)
Virus shellShell ID: 1 / Diameter: 1000.0 Å / T number (triangulation number): 13

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Macromolecule #1: Wedge 2 protein

MacromoleculeName: Wedge 2 protein / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 54.432738 KDa
SequenceString: MAENYGLTGS GFNLPPMDDL VQETKKTFKS AFGEDFNTES NSVADKLIQI FNEREYQLWL LMGSVYYAQT MQGAEGIYLD DLLGKRGIY RLGKTRSTGT VVMTIDSSVP YNMIYSAATY TIDTDYELSS DVQVAGNIVA QLIKGTDLSV GTYRLQIQNT T DQSVKTLS ...String:
MAENYGLTGS GFNLPPMDDL VQETKKTFKS AFGEDFNTES NSVADKLIQI FNEREYQLWL LMGSVYYAQT MQGAEGIYLD DLLGKRGIY RLGKTRSTGT VVMTIDSSVP YNMIYSAATY TIDTDYELSS DVQVAGNIVA QLIKGTDLSV GTYRLQIQNT T DQSVKTLS LNLTATSGQP LITFFGQIKD FIVNNTILSN QDRIWIDSTE GALYIGYDTN KIMIGLSSRV DFRTNPMAGT RS ISMDVRS IEPGYISRDV HSVRSINPTP GGFVDIDNLS AFIDGSDVES DNEYRIRAAT SISEGKATRP AILAALLNKV EGI EKVRIF NNNTDKTNSL GIPPYRFMVV CYGGGTAEIS QVLYDTIATS NNTYGDTFYD ITTEDDQVER IWHTKAAARQ LAIR VRYRG RPLSLTEETA IANGLATAVN GTMIAGTLYN VRLVGTVMSS TSPDRFTQVY VDIKNKGQPD SAYVNTDVTA STTQV LSLE LEDVIFSQIV

UniProtKB: Putative baseplate assembly protein

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Macromolecule #2: Tail sheath protein

MacromoleculeName: Tail sheath protein / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 50.647766 KDa
SequenceString: MAEYQDKVVD VEVSLGTQPI DTVGFETPMF LAMHGNFPER IRFYVSTAGM VADGFAVGSP AYQFATNAFA GNFAPQRVAI GRMSIDSSK VDFTGTTNTE QVVVNITLNK VVKAVKINVL PGNTPAQIAT ALADAVTADA DLTGKATAVA TGTYVTVTAV S PNVVSVGK ...String:
MAEYQDKVVD VEVSLGTQPI DTVGFETPMF LAMHGNFPER IRFYVSTAGM VADGFAVGSP AYQFATNAFA GNFAPQRVAI GRMSIDSSK VDFTGTTNTE QVVVNITLNK VVKAVKINVL PGNTPAQIAT ALADAVTADA DLTGKATAVA TGTYVTVTAV S PNVVSVGK GAGVYKIVNE SSETVATVLP SVIAENHNWY FLATEARSDA DIVAAAEFAK ANYKLHIYNS TDVDAYAPEN SA ASVFDTL KSLSYDSLGT SDAGADVDFT EGSVIGAMAA NDPSYGDSLH LKTMPGMVPF AGSDTQRSNA WSRNANIYRG LYG GGSYIE GKTSSGQYVD VIRFSHWVKF RMEESVFAYM KRRSDMGLSM KMSDEDLPVL KSVLMNNPIN IGIRNGGILT GYDT ENKVS YDPTIIIPKR ANIPTNDLAA RILRDVKVEL VYNNSLHYVK IRASVVLDRP AGQSTNAQTP MSSSAVGV

UniProtKB: Structural protein

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Macromolecule #3: Tail tube protein

MacromoleculeName: Tail tube protein / type: protein_or_peptide / ID: 3 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 16.79285 KDa
SequenceString:
NQSKILTLQA YDPAKVLVFI GGQRVSGFAA DTKIVITRNN DNISVHAGVD GEISNALSRD NTGVMTLSLQ NTAKWNGYLA QWQRQANVT GLIYLPVQVE GSQGLSLNTI GWIQKQPDLS YGTEVGQMDW EIGVLDAWLS PDQIQGIAAG ITGLLGL

UniProtKB: Structural protein

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Macromolecule #4: Tube initiator protein

MacromoleculeName: Tube initiator protein / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 31.720324 KDa
SequenceString: MAVNNNSISP AQQAQADKRA RDAAAAKRKQ DTEVSNAKSR EDIQYTMFVS GLRRGRLNEF ERKNRTDDLA ILFDSVTTHT YTKDYNKSS YAVESKAKAS DHVTTQDGKF TFSGTVTDSP YLIDPRNMID RDTDKENPML ARRPAKAIEI LELIADSHQL V TLVTEDNI ...String:
MAVNNNSISP AQQAQADKRA RDAAAAKRKQ DTEVSNAKSR EDIQYTMFVS GLRRGRLNEF ERKNRTDDLA ILFDSVTTHT YTKDYNKSS YAVESKAKAS DHVTTQDGKF TFSGTVTDSP YLIDPRNMID RDTDKENPML ARRPAKAIEI LELIADSHQL V TLVTEDNI LSNYVITSFQ VDRSSEAGSS INVQVTLEEF RFKMVNKTVM ARTSDPKKAK NANTGTKQTA EDGAVDDSAK QK RQTPYIG KNAETKERWE NAAIGTTDFS GKPGAKLPFD PSDLMRK

UniProtKB: Dit-like phage tail protein N-terminal domain-containing protein

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Macromolecule #5: Hub protein

MacromoleculeName: Hub protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 36.545242 KDa
SequenceString: MRAMERTYTL VIGRPVVIGE KPVNIEKFAN TSKGDAYEIK DLHIEFNVKK DNSKEPNKGY VTVYNLSDEV VNYLSVNQRE SLAVMLHAG YNGDEKLIFS GTVEYVEDDF PEETRTTKFI LGDGTLNLTT ATTARSYRKG TPVNSVLNDL IADLKLPKGR V IDFGNQTL ...String:
MRAMERTYTL VIGRPVVIGE KPVNIEKFAN TSKGDAYEIK DLHIEFNVKK DNSKEPNKGY VTVYNLSDEV VNYLSVNQRE SLAVMLHAG YNGDEKLIFS GTVEYVEDDF PEETRTTKFI LGDGTLNLTT ATTARSYRKG TPVNSVLNDL IADLKLPKGR V IDFGNQTL QTSMAFTGNA SQNLANLAKN TGSTFSVQDG AVYWTKEGSR FNNVMFEISE EGGMVGTPTP KQPSSSKKLI KA KAKAKSD EAEGKKPKAP SKKKKEHDIK EDVGMTVSTL LNGAILPEST VYLNTRYHKG FYKVAELTHR GGYETGDWIT ELG LVETRG ELIK

UniProtKB: Tail protein

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Macromolecule #6: Putative baseplate assembly protein

MacromoleculeName: Putative baseplate assembly protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 26.474344 KDa
SequenceString: MAYADRYDAA FGIYISRFLR NNVHTNIRGK VVGVNYSGPS VDVQPMAYTE FPSGTTDRYP VIYDVPVLLP SGAGGKARLT MPIKPGDVV GLSFSERNEG DNNDQNTHQL FAGWAVTQIF TDGNSKAIHP DNVVLENDKA IITLKPDGDT SLQNPKVTVE A LADGNVKI ...String:
MAYADRYDAA FGIYISRFLR NNVHTNIRGK VVGVNYSGPS VDVQPMAYTE FPSGTTDRYP VIYDVPVLLP SGAGGKARLT MPIKPGDVV GLSFSERNEG DNNDQNTHQL FAGWAVTQIF TDGNSKAIHP DNVVLENDKA IITLKPDGDT SLQNPKVTVE A LADGNVKI SNGTGEFTMS PSGEIKGGNR GGSFTLQPSG QLMCNGARIT TSGRIITANG VDMDDFYQKY MSHTHGGVSR GG DNTNPPN

UniProtKB: Putative baseplate assembly protein

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Macromolecule #7: Wedge 1 protein

MacromoleculeName: Wedge 1 protein / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 18.869457 KDa
SequenceString:
LKDVNHIHPL PDFVRGGFDY VPSDIFSGKE NFEQTLRILL ERLEFIDQKM VELAELRTTL NAEDAILDEI GRQLGIYRNG LNDPEYRAV IMILTGNNSK SGTRADIIAT LKQLFGEDGV TTYKGYNYRL DINIFNTCME VTDILPEIID MLPLVTHLRV V ENQGY

UniProtKB: Uncharacterized protein

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Macromolecule #8: Spacer protein

MacromoleculeName: Spacer protein / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 13.055748 KDa
SequenceString:
RTVVRTFDFE LSGYPDETFR VVLDSVTYEL RFMWNERDES WFMSLGDIGA QRPTITSKLT CYSDILAPYR YLDNVPDGNL YLWPLGDIR TRAGRFNIGP LKGIQMTYSS LIE

UniProtKB: Cyanophage baseplate Pam3 plug gp18 domain-containing protein

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Macromolecule #9: Sheath initiator protein

MacromoleculeName: Sheath initiator protein / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 18.823365 KDa
SequenceString:
QFKDLLLDPL TGDLDFGTPG DRGMRLALTN QLSLRQRLYL RFAIWAGDWY FDETFGFPYR TFVGKKTVKA VLDGRIKSEV RQEPDVLQI TDFQSTMDVV SRSYKCFFTV VTAEGEEISL AFVGEDEYQY PTPPESNVQL CGDEGVIINF KNKLYYLINF R LPK

UniProtKB: Uncharacterized protein

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Macromolecule #10: Putative tail fiber protein

MacromoleculeName: Putative tail fiber protein / type: protein_or_peptide / ID: 10 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 81.935617 KDa
SequenceString: MSSNTDIIAL LSSDADKAVD QIIEDSKRLH IVVNGTGTEQ AVAEDGSLLP SVRKALIDNL YFKTPPLPWR NGGSVNEFNQ LYSFTDVSG NTTWWYAPGA TVSNPVVMRD SPINDGKFKV FLDKTNIADI YAPLNSPNFV GNPRVPTPAP GDNSQSIPTT G WVQSEMEG ...String:
MSSNTDIIAL LSSDADKAVD QIIEDSKRLH IVVNGTGTEQ AVAEDGSLLP SVRKALIDNL YFKTPPLPWR NGGSVNEFNQ LYSFTDVSG NTTWWYAPGA TVSNPVVMRD SPINDGKFKV FLDKTNIADI YAPLNSPNFV GNPRVPTPAP GDNSQSIPTT G WVQSEMEG LEDLIISSMR GEFENITVRN DAILQDTYIN GEFIVTSDKL AAANTEATFR RIRMVGTGTT GTAATEIAFE QN TTPPPGV STKTNIKPFA VSTGILTVDQ INGKAAQVGD VAVSTDSLNV DGYMRGDYLH LEGNNRNTAG RPQLIVDGIA EIE TLRVTG TIEGVKADVD GLDIHPRSVI VDEGLTVAKD TTMTGKLTVG GTTKVGNLEV TGTLSGVAIS VDGKDIKPRS VNTG NLVAT GDVQISGTTT ATGKITTQDL EVLGTLTANL DLSGSDLSVD NINVAQTATI TDLVVTGTTT GVKGDVEGEA IAPAS VAAT GTVSAASMST TGNMAVGGNL TITGDFRPAS VAGQDTPRLV VQGDSDLQGD LNVSGTITGV VDLTAQDINM NSLGLA QGL TAGGGITSFS TVKGVNGVFG EGSTAPGAIG LQSMTNAKVA TDLLVGEDLR VEGDTNLFGN LSVTGTITGN MNFSGKT IA PATVNATTVT ATNLTVTGTI DLTDSPIVAQ EITATNVEAT GKMVAGKYST TPKTANATTA TFTPDGTTSI YNVNVQAD T EVQAPAGLLS SGKGESIFIY FEQDTTGHAV TFSSAFVVHN AATVSNAANS ITIANLVYRG TGPLIDVFLT GR

UniProtKB: Putative tail fiber protein

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Macromolecule #11: Short tail fiber

MacromoleculeName: Short tail fiber / type: protein_or_peptide / ID: 11 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 59.236 KDa
SequenceString: MAKPKDPIYV WAAADVNLPG TGRPNKSKPI DDLLAKGYDK GQKPAAEEFN YILNMSSAWV NWIVNEKFPE LEAEIARLLA ELENRINQQ LAVIRQDIAQ LRQDVADLRR YVDQKVQELK QEIQGVRNDL NKLRQDFDAA ITQVNGRIDD LEPRLVPIGA V IPWPGATV ...String:
MAKPKDPIYV WAAADVNLPG TGRPNKSKPI DDLLAKGYDK GQKPAAEEFN YILNMSSAWV NWIVNEKFPE LEAEIARLLA ELENRINQQ LAVIRQDIAQ LRQDVADLRR YVDQKVQELK QEIQGVRNDL NKLRQDFDAA ITQVNGRIDD LEPRLVPIGA V IPWPGATV PDGWLECSGQ VFNTGQNPKL YSVLGRNVVP DYRGLFLRGW AHGSDANDPD AGRALGSVQG DAIRNITGYF PA DIGNSNF IGRYVGGAFR DDGSLTSGDD GTRSNEVRKY SFDASREVPV AAENRPKNIA VMYIIKTDQA QSSGGNSPTA IVV SPDTIT NRIGYAVKAT ASVLPASISG QYPISWSTQN AAVATVDGSG NISLVGPGET NIIASISTGM NVVIRVTSYS VLTS ISIAD PGQITVTESK MLVVTKSPSS ANEPLQFLSN NSGVAAVNDA GYVIGVSAGS ATITVRGTLS GVSSTRAVTI IPEQV EESV QDNRLGAVGS YIPPSPSSNV MSWTFQAPQG CALTGIIVQE NESNSGDNIG GVYYKPIQKK VNGVWVTITG

UniProtKB: Uncharacterized protein

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Macromolecule #12: Putative tape measure protein

MacromoleculeName: Putative tape measure protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pectobacterium phage phiTE (virus)
Molecular weightTheoretical: 87.898227 KDa
SequenceString: MAASGNVVVT RTTNRVTFET DKASYARAVK QIRQVGKEWE KASDAISKPK KDPAKAYDKS AQQMRLVNKR LAETRAREEK RASERSIAL ARREAKAKEA IARVSAARIK QQVQQMTSPR AGMSEMKKFY QQQEREAKKA NRKATIGMNA ASRPMNVTRI N SAPLSPRG ...String:
MAASGNVVVT RTTNRVTFET DKASYARAVK QIRQVGKEWE KASDAISKPK KDPAKAYDKS AQQMRLVNKR LAETRAREEK RASERSIAL ARREAKAKEA IARVSAARIK QQVQQMTSPR AGMSEMKKFY QQQEREAKKA NRKATIGMNA ASRPMNVTRI N SAPLSPRG GQGTGMVGDP NKVYNPELIA AQNRAMAGRI RSQSQAQDRK AKAEARQRAA AAARAARIDD VMAQQRIRLS SK YGRSYEG RLGRDGAGQG IQDLNRQFRA GTLSAGQYRQ SIQALERQFR SAQANAGGFG AALGDIRSQL LNAGAAYGVF ASG ASVLKQ GQFFQGMEAT MSMVSDSSEE AGQRIKFVKD QAYRLGLDLK IASQGYTQMA VNSAGILSKA QNDDLFKGFS EFAT ASQVD PVKFQRGITA IGQMMGKGQV MAEELKGQLA EGIPGSLQVF VKAAQEAFGD TTIDVEKLMD MMQKGELKAA KILPF VGKY FAEAARKGGA LPKALESNRV AMQRLSLTWI DFQNQIFQGG FGEQMTRVFR DLATILDSNG ELATNLGAFF GNVIEG FWD MVTEIHDDFV LLDRIVSYYT EKLGYQGDLL KEVFDWAGYA IGIGIFVGGL NKIFKILTKI AGLRTALVGV RQAMGGA IT RGGVTNGAGL GGGAGAAGTP GASRTASFLS KWKGLSKISK VGILGTLYSA GSMLNEQFIK RGDDKLADAG LDQETFQK E YGFMPKPVGL LDVFDKWFNK PRDLDAPTIT APGPGSGMAA QTGIPFPAPQ KVEGDITIKI EAGELRNMVR AVVDENNQF NFNMLIQGGP N

UniProtKB: Putative tape measure protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 4 K

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: OTHER / Details: Ab initio helical model generated in cryoSPARC
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.24 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 16480
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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