EMDB-45802: Cryo-EM structure of porcine brain ventricles cilia doublet micro...

基本情報

登録情報

データベース: EMDB / ID: EMD-45802

• タイトル: Cryo-EM structure of porcine brain ventricles cilia doublet microtubule (48-nm periodicity)

試料

• 細胞器官・細胞要素: cilia from porcine brain ventricles
  • タンパク質・ペプチド: x 41種
• リガンド: x 2種

• キーワード: axoneme / cilia / microtubule / dynein / brain ventricle / doublet microtubule (DMT) / STRUCTURAL PROTEIN

機能・相同性

分子機能:

outer dynein arm docking complex / regulation of cilium beat frequency / determination of heart left/right asymmetry / protein localization to motile cilium / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / mucociliary clearance / axonemal B tubule inner sheath / axonemal A tubule inner sheath / inner dynein arm assembly / regulation of calcineurin-NFAT signaling cascade / sperm axoneme assembly / regulation of microtubule nucleation / positive regulation of feeding behavior / outer dynein arm assembly / cilium-dependent cell motility / 9+0 non-motile cilium / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / cerebrospinal fluid circulation / epithelial cilium movement involved in extracellular fluid movement / 9+2 motile cilium / regulation of store-operated calcium entry / intraciliary transport / regulation of cilium assembly / axoneme assembly / microtubule sliding / cilium movement / ciliary transition zone / left/right axis specification / calcium ion sensor activity / axonemal microtubule / ciliary tip / gamma-tubulin ring complex / ciliary rootlet / ventricular system development / manchette / positive regulation of cilium assembly / flagellated sperm motility / UTP biosynthetic process / CTP biosynthetic process / determination of left/right symmetry / intermediate filament / positive regulation of cell motility / nucleoside diphosphate kinase activity / motile cilium / GTP biosynthetic process / seminiferous tubule development / ciliary base / receptor clustering / regulation of neuron projection development / cerebral cortex cell migration / microtubule organizing center / regulation of cell division / mitotic cytokinesis / homeostasis of number of cells / axoneme / cilium assembly / glial cell projection / spermatid development / alpha-tubulin binding / sperm flagellum / single fertilization / beta-tubulin binding / microtubule-based process / cytoplasmic microtubule / sperm midpiece / Hsp70 protein binding / 3'-5' exonuclease activity / centriole / acrosomal vesicle / lung development / cellular response to leukemia inhibitory factor / mitotic spindle organization / meiotic cell cycle / Hsp90 protein binding / G protein-coupled receptor binding / brain development / SH3 domain binding / structural constituent of cytoskeleton / centriolar satellite / microtubule cytoskeleton organization / spindle / multicellular organism growth / cell morphogenesis / spindle pole / mitotic spindle / intracellular protein localization / nuclear envelope / mitotic cell cycle / heart development / actin binding / microtubule cytoskeleton / cell body / spermatogenesis / microtubule binding / gene expression

ドメイン・相同性:

: / : / Outer dynein arm-docking complex subunit 4 / Protein of unknown function DUF4586 / Outer dynein arm-docking complex subunit 3 / Cilia-and flagella-associated protein 96 / FAM183 family / FAM183A and FAM183B related / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / : / : / Protein CFAP95 / ODAD1 central coiled coil region / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / Sperm acrosome-associated protein 9 / : / Tektin family / Protein of unknown function (DUF3695) / Sperm acrosome-associated protein 9 / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / EFHB C-terminal EF-hand domain / Nucleoside diphosphate kinase 7 N-terminal PH domain / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Recoverin family / Parkin co-regulated protein / Parkin co-regulated protein / EF hand / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase (NDPK)-like domain profile. / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / HEAT repeats / Armadillo/plakoglobin ARM repeat profile. / Armadillo/beta-catenin-like repeat / Armadillo/beta-catenin-like repeats / Armadillo / Tetratricopeptide repeat / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / TPR repeat profile. / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tetratricopeptide repeats / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Tetratricopeptide repeat / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site

構成要素:

Cilia- and flagella-associated protein 53 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 45 / Outer dynein arm-docking complex subunit 2 / Sperm-associated antigen 8 isoform 1 / Tektin / Tektin / Piercer of microtubule wall 2 / Meiosis-specific nuclear structural protein 1 / Enkurin / Calaxin / Tektin / Cilia-and flagella-associated protein 96 / Tubulin beta chain / Tubulin alpha chain / Outer dynein arm docking complex subunit 3 / Cilia and flagella associated protein 161 / Parkin coregulated / Cilia and flagella associated protein 276 / Cilia and flagella associated protein 95 / Nucleoside diphosphate kinase homolog 7 / Cilia and flagella associated protein 141 / RIB43A-like with coiled-coils protein 1 / Sperm acrosome associated 9 / Tektin / Ciliary microtubule inner protein 2B / Protein Flattop / Piercer of microtubule wall 1 / Ciliary microtubule inner protein 2B / Outer dynein arm-docking complex subunit 4 / Cilia and flagella associated protein 107 / EF-hand domain family member B / Cilia and flagella associated protein 77 / Cilia and flagella associated protein 210 / Cilia and flagella associated protein 144 / RIB43A domain with coiled-coils 2 / Cilia- and flagella-associated protein 52 / Cilia-and flagella-associated protein 20 / Coiled-coil domain-containing protein 114 isoform X2 / EF-hand domain-containing family member C2

• 生物種: Sus scrofa (ブタ)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.65 Å
• データ登録者: Sun C / Zhang R

資金援助

米国, 1件

Organization	Grant number	国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	1R01 GM138854	米国

• 引用: ジャーナル: Nature / 年: 2025; タイトル: Structural diversity of axonemes across mammalian motile cilia.; 著者: Miguel Ricardo Leung / Chen Sun / Jianwei Zeng / Jacob R Anderson / Qingwei Niu / Wei Huang / Willem E M Noteborn / Alan Brown / Tzviya Zeev-Ben-Mordehai / Rui Zhang / ; 要旨: Reproduction, development and homeostasis depend on motile cilia, whose rhythmic beating is powered by a microtubule-based molecular machine called the axoneme. Although an atomic model of the axoneme is available for the alga Chlamydomonas reinhardtii, structures of mammalian axonemes are incomplete. Furthermore, we do not fully understand how molecular structures of axonemes vary across motile-ciliated cell types in the body. Here we use cryoelectron microscopy, cryoelectron tomography and proteomics to resolve the 96-nm modular repeat of axonemal doublet microtubules (DMTs) from both sperm flagella and epithelial cilia of the oviduct, brain ventricles and respiratory tract. We find that sperm DMTs are the most specialized, with epithelial cilia having only minor differences across tissues. We build a model of the mammalian sperm DMT, defining the positions and interactions of 181 proteins including 34 newly identified proteins. We elucidate the composition of radial spoke 3 and uncover binding sites of kinases associated with regeneration of ATP and regulation of ciliary motility. We discover a sperm-specific, axoneme-tethered T-complex protein ring complex (TRiC) chaperone that may contribute to construction or maintenance of the long flagella of mammalian sperm. We resolve axonemal dyneins in their prestroke states, illuminating conformational changes that occur during ciliary movement. Our results illustrate how elements of chemical and mechanical regulation are embedded within the axoneme, providing valuable resources for understanding the aetiology of ciliopathy and infertility, and exemplifying the discovery power of modern structural biology.

履歴

登録	2024年7月18日	-
ヘッダ(付随情報) 公開	2024年10月23日	-
マップ公開	2024年10月23日	-
更新	2025年3月19日	-
現状	2025年3月19日	処理サイト: RCSB / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_45802.map.gz / 形式: CCP4 / 大きさ: 512 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• ボクセルのサイズ: X=Y=Z: 1.34 Å

密度

表面レベル	登録者による: 0.35
最小 - 最大	0.0 - 2.3817267
平均 (標準偏差)	0.011283159 (±0.07916241)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 512 512 512 Spacing 512 512 512
セル	A=B=C: 686.08 Å α=β=γ: 90.0 °

添付データ

マスク #1

• ファイル: emd_45802_msk_1.map

ハーフマップ: #2

• ファイル: emd_45802_half_map_1.map

ハーフマップ: #1

• ファイル: emd_45802_half_map_2.map

試料の構成要素

全体 : cilia from porcine brain ventricles

• 全体: 名称: cilia from porcine brain ventricles

要素

• 細胞器官・細胞要素: cilia from porcine brain ventricles
  • タンパク質・ペプチド: Armadillo repeat-containing protein 4 isoform 1
  • タンパク質・ペプチド: Outer dynein arm-docking complex subunit 4
  • タンパク質・ペプチド: EF-hand calcium binding domain 1
  • タンパク質・ペプチド: Coiled-coil domain-containing protein 114 isoform X2
  • タンパク質・ペプチド: Outer dynein arm docking complex subunit 3
  • タンパク質・ペプチド: EFCAB6
  • タンパク質・ペプチド: Cilia- and flagella-associated protein 52
  • タンパク質・ペプチド: Cilia- and flagella-associated protein 45
  • タンパク質・ペプチド: Cilia and flagella associated protein 210
  • タンパク質・ペプチド: Cilia and flagella associated protein 276
  • タンパク質・ペプチド: Enkurin
  • タンパク質・ペプチド: Parkin coregulated
  • タンパク質・ペプチド: Cilia- and flagella-associated protein 20
  • タンパク質・ペプチド: Protein Flattop
  • タンパク質・ペプチド: Tektin
  • タンパク質・ペプチド: Tektin
  • タンパク質・ペプチド: Tektin
  • タンパク質・ペプチド: Tektin
  • タンパク質・ペプチド: RIB43A domain with coiled-coils 2
  • タンパク質・ペプチド: EF-hand domain containing 1
  • タンパク質・ペプチド: EF-hand domain-containing family member C2
  • タンパク質・ペプチド: Ciliary microtubule inner protein 2B
  • タンパク質・ペプチド: Ciliary microtubule inner protein 2B
  • タンパク質・ペプチド: Sperm-associated antigen 8 isoform 1
  • タンパク質・ペプチド: Nucleoside diphosphate kinase homolog 7
  • タンパク質・ペプチド: Cilia and flagella associated protein 161
  • タンパク質・ペプチド: Chromosome 1 C9orf135 homolog
  • タンパク質・ペプチド: Piercer of microtubule wall 1
  • タンパク質・ペプチド: Chromosome 1 C15orf65 homolog
  • タンパク質・ペプチド: EF-hand domain family member B
  • タンパク質・ペプチド: Cilia and flagella associated protein 141
  • タンパク質・ペプチド: Meiosis-specific nuclear structural protein 1
  • タンパク質・ペプチド: Cilia- and flagella-associated protein 53
  • タンパク質・ペプチド: CFAP107/C1orf158
  • タンパク質・ペプチド: Cilia and flagella associated protein 77
  • タンパク質・ペプチド: Cilia and flagella associated protein 144
  • タンパク質・ペプチド: Cilia-and flagella-associated protein 96
  • タンパク質・ペプチド: Sperm acrosome associated 9
  • タンパク質・ペプチド: Tubulin alpha chain
  • タンパク質・ペプチド: Tubulin beta chain
  • タンパク質・ペプチド: RIB43A-like with coiled-coils protein 1
• リガンド: GUANOSINE-5'-TRIPHOSPHATE
• リガンド: GUANOSINE-5'-DIPHOSPHATE

超分子 #1: cilia from porcine brain ventricles

• 超分子: 名称: cilia from porcine brain ventricles / タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 / 含まれる分子: #1-#19, #21-#41, #20
• 由来(天然): 生物種: Sus scrofa (ブタ)

分子 #1: Armadillo repeat-containing protein 4 isoform 1

• 分子: 名称: Armadillo repeat-containing protein 4 isoform 1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 116.987055 KDa

配列

文字列:

MGVALTRSAQ WTAAGHGTRN LEITPLNEAI LKEIIIFVES FIYKHPQEAN YVFVEPLEWQ TKLDPSAFGS GYAVSETTVK SEEVDKNGE PLLFLSVPQI KIRSFGQLSR LLFIAKNTKL KEAQACIEAN RNPITKILGL DYNVINEINE DSTLLTILDK I TKDEDPES ETKVKIALLL KQLDLHLLNH SLRHISLEIR LNPRTVKNDI ELLKKFSGKG EQTVLESIDY TSDYEFSNGC RA PPWRQMQ GEICYVLVKP HDVEILCLTC SNEGVFLNGG KTDDEGEINY ERKGEIYKDL VTLLKEKSAV FSENMSKQEV RFS EEQQKE KDQLNEVPKE EEAAALHKAS NASHRSNLEK NQINLGKNQM TKRLEPSLNW KTTVDAKEKR RLQRDNLEEK RGGR LEKPR PSILPGRAQL IRRSIEKAEE VISESSSESE EDEEPPDHRQ EANADLPSEY WQIQKLVKYL KGGNQTATVI ALCSM KDFN LAQETCQLAI RDVGGLEVLI NLLETDEVKC QIGSLKILKE ISHNPQIRRN IVDLGGLPIM VNILDSPHKS LKCLAA ETI ANVAKFRRAR QAVRRHGGIT KLVALLDCGQ NLPEPAYLSL YETRDVEVAR CGALALWSCS KSYANKEAIR KAGGIPL LA RLLKTSHQDM LIPVVGTLQE CASEENYRAA IKAERIIENL VKNLNSENEQ LQEHCAMAIY QCAEDEETRD LVRLHGGL K PLASLLNNTD NKERLAAVTG AIWKCSISKE NVIKFREYKA IETLVGLLTD QPEEVLVNVV GALGECCQEH ENRVIVRRC GGIQPLVNLL VGINQALLVN VTKAVGACAV EPESMMIIDR LDGVRLLWSL LKNPHADVKA SAAWALCPCI QNAKDAGEMV RSFVGGLEL VVNLLKSDNK EVLASVCAAI TNIAKDEENL AVITDHGVVP LLSKLANTNN DKLRRHLAEA ISRCCMWGRN R VAFGEHKA VAPLVRYLKS SDTNVHRATA QALYQLSENA DNCITMHENG AVKLLLDMVG SPDQDLQEAA AGCISNIRRL AL ATEKARY T

UniProtKB: Outer dynein arm-docking complex subunit 2

分子 #2: Outer dynein arm-docking complex subunit 4

• 分子: 名称: Outer dynein arm-docking complex subunit 4 / タイプ: protein_or_peptide / ID: 2 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 78.707766 KDa

配列

文字列:

MAEQEANGLR STFPSYMAEG ERLYLCGEFA KAAHSFSNAL HLQNGDKNCL VARSKCFLKM GELEKSLADA EASLQGDPTF CKGILQKAE TLYTMGDFEF ALVFYHRGYK LRPDREFKVG IQKAQEAINN SVGSPASIKL ENREDLSFLS KQAERMQAQQ K AHPVRQFL YHPKRESKRK GSLKSEKIVR QLLGELYVDK EYLEKLLLDE DLIRGTIKHG LTVEDLIMTG INYLDTRSNF WR QQKPIYA RERDRKLMQE KWLRDRKRRP SQTAHYILKS LEDIDMLLTS GSAEGSLQKA EKVLKKVLEW SKEEVPNKDE LLG NLYSCI GNAQMELGQM VAALQSHRKD LEIAKEYDLP EAKSRALDNI GRVFARVGKF QQAIDTWEEK IPLARTTLEK TWLF HEIGR CYLELDQAWQ AQNYGEKSQQ CAEEEGDIEW QLNASVLVAQ AQVKLRDFES AVNNFEKALE RAKLVHSNEA QQAII SALD DANKGIIEEL KKTNYRDILK DKKEKEDVLD NTSAKEKEAT RRVRDEPEKV MKRWEHEQME NEKEMEGDLS KDTLGT AAS GQEGHEQRGA IEKASPLRRT EQAAMGRDPG EAAKEHLGEA GRTLSELKRG ETREIYRRPS DLDQNLSEEL STREPEV LE KKLSETGRGE SERLEKTESE EIGETENTEE MRKDEKEDEL ALE

UniProtKB: Outer dynein arm-docking complex subunit 4

分子 #3: EF-hand calcium binding domain 1

• 分子: 名称: EF-hand calcium binding domain 1 / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 29.379803 KDa

配列

文字列:

MNSVCLGKDT ALTLGRSQQL SVALMGVNLT MDACRRPGAA VCKLTCAELK LGIKDGLNAC CYICIFSFTV NKFEVKCLIS LFYNLVGDV TERQGVVVGL DRNAFRNILH VTFGMTDDMI MDRVFRGFDK DNDGCVSVSE WVYGLSVFLR GTLEEKMKYC F EVFDLNGD GFISKEEMVH MLKNSLLKQP SEEDPDEGIK DLVEITLKKM DHDHDGKLSF PDYEAAVREE ALLLEAFGPC LP DPKSQKE FEAQVFKDPN EFGEV

UniProtKB: Calaxin

分子 #4: Coiled-coil domain-containing protein 114 isoform X2

• 分子: 名称: Coiled-coil domain-containing protein 114 isoform X2; タイプ: protein_or_peptide / ID: 4 / コピー数: 3 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 80.337516 KDa

配列

文字列:

MPLGLSAGST RSEDGSEAFL EGMVDWELSR LQRQCKVMEG ERRAYSKEVH QRINKQLGEI QRLEGVRDKL QVQISIAQSQ VKRLRDSER LENMGHLLKC RVQVQAEVKE LQEQTRALDR EIQEWETRIF AHSQDVKAPG CVLSQKVKVR QRIKILEDQL D RVTCRFDI QLVRNATLRE ELDLLRIERN RYLNVDHKLQ KEIQHLRHAV SALMVSSTSA YTVREEAKNK ISMLRERAEK EV AQNETEV QILQRQVAHL EQLHRFLKLK NDDRQPDPAI LAKREQRARE VAEGLRKTSQ EKLVLRYEDA LNKLYQLTGE SDP DLMVEK YLELEERNFA EFNFINEQNS ELEHLQEEIK EMQEALVSRR RSEEDRRAQQ EQQRTELQQR VDEVHTEAEN LEAR FQDFR GQLEALKTAI QRLFTRAQCD STLINDLLGV KTQMRDRDIG LFLGLIEKRL VELLMVHAFL ETQSYPFTSL PSAAL MVLG QSPEELLKKV VLPLPPDNLE EPPGFEARDD YPLSKEELLS QVVKSLKVQE QAREQHLKEL AEAVKKVDST PSITFS QKA SSSTRLLPKS PSAIPGSIMS HRTSGILVAG GGRAPSSNVG HVTFGEPSSS AGRMTYGSTS ATGALASSRS STGGRVT FR SPSSSSYLGS TGYLESSRGH ESFGGLESRG PGSESSGGLG FSRGQVSSTG PASSTGQGST TSKDSRSNY

UniProtKB: Coiled-coil domain-containing protein 114 isoform X2

分子 #5: Outer dynein arm docking complex subunit 3

• 分子: 名称: Outer dynein arm docking complex subunit 3 / タイプ: protein_or_peptide / ID: 5 / コピー数: 3 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 72.117602 KDa

配列

文字列:

MTSPLCWAAS ANAMPSQDQV SASSKGRGNQ AQAKGHPQGK GSVQAWQSLH SKAGPFHASE GKSTVHTQVA ELQRKIQLLE GDRKAFYES TQWNIRKNQE TINQLREETR VLQLQLADLL QGDEKVVQAV IREWKSEKPY LKNRTGQQAL EHLDHQLSEK V KQLNALRH QLGLRQKWLE ELQLQHSLRE LEMAEAQDRN TEVAKTMRNL ENRLEKALMK AEEAEHITNV YLQLKAYLQE ES LHLENRL DFMEAEVVRT KHELEELNVV NQEALNARDI AKNQLQDLEE TVLRERKKRD RYLTECKKRA EERKLQNERM ERK TQREDV LLHSDDTLQD SQRSKEEELR RRWSMYQMEV LFGKVKDATG VAETHAVVRR FLAQGETFTQ LETLKIENEQ MLLR LKQEK QRLQKELEDL KYSGEAMLMS EQKLQVELQE RIKAEEQRRA DVQDRLERTM RAMHMTKEAL EHLASKLDHI TVADS APEE APPRAPQDVR GSSTITQEAS GFAGKELDPK ASDYLPNLLG LVEEKLLKLQ AKLQNHNVPE MLRHIADREF FSTLEG KLP SYNTRIALPL AGHKDKFFDE EESEEEDNEV VTRAALKMRS QKLIESRSKR RGRSRKS

UniProtKB: Outer dynein arm docking complex subunit 3

分子 #6: EFCAB6

• 分子: 名称: EFCAB6 / タイプ: protein_or_peptide / ID: 6 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 167.103703 KDa

配列

文字列:

MSTPSSTTAM ANPILSSLDV KRILFQSITN KGDDLKKAFH LLDTGNDRTV SKNELRRVIA DFLLPLTREQ FQDILAQIPL TRAGAVPYL EFLSRFGGTD LNVNAVKRGG ENAMNHCRTL RDLEAQVAEK IFKNIHTVAK AFKLLDVNKT GLVQARELRR V LETSVRGL LDERFYFRFA KHYNVDKDTA VDYNVFLKNL GTNNDLNLKY DVGSQEVSWE EQRAKNPSRE CLPSSLSPED IR GNYSLDD LERVFCQELS RSHERVEKAL SAGDPSRGGY VSLNYLKIVL DTFVCRLPRR IFIQLMKRFG LKTTAKINWK QFL TSIYEP QWLETSNKIP MTKRNSINSR QPRQENIIPR LFSHEDHSTA LKEALLIINS KPGRQITGEE LRHTLNDMAV KISD SEFKE LMRTLDPGST GWVNVSTFVE LLEEKPRLSK PSPCEDPKAP LLLAWDSVEE IVHHSVTRNL (UNK)GFRRMLQSC D LGDTGLVG RQNFKKIMRA FCPCLTAEHL VKLCNKFQDI TSGRILYKKL LACLGINGPP TVSPVLVPKD QLFNEHFQKE DQ QQPDLSE RTEPTESKSA VTKKVTKEEV IEKLKDCIRQ RDPAFRKRFL DFSKEPNGKI NAHDFRKVLE EHGMPMDDDQ YAL LTAKIG YKKEGMSYLD FATGFEDAAM NGPEATPPQT PGLSKGTSDS SLITAKECLR QFPRRLKEFF RDPYAAFFNL DLDR DGLIS MHDLRRLLLH LRFNLKEEEF ERFLGLLGLR LSVTLNFREF RNLFEKRPFR SEDDAPQRLL RPKQKVADSE LACEQ AHQY LVTKAKTRWA DLSKNFIETD SEGNGILRRR DVKNALYGFD IPLTPREFEK LWLSYDTEGR GHVTYQEFLQ KLGISY SAD VHRPYANDHF NFMGHFTKQQ QMHEELRELQ QSAEKAVPPR DKLKDHYQDI SKALSKLDKP KNGSISFCKL QKLLQEC GC SLKQEELTDL LNSWGISWHD NSISYLDFLR AVENSKTTRP QPREKKERVP LDFATLNPEE VLKSIQEVVA ASGQALST A FSALDKEDTG FVKASDFGQV LKEFCYKLAD NQYHYFLRKL RLHLTPHINW KYFLQNFSSY LEETAAEWAE KMPKGPPPR SPKETANQAI LARLHKAVAS RYHAIAREFD GFDTAKTNTA SRDEFRSICT RHVQVLTDEQ FDRLWEEMPV NA(UNK)GRL KYL DFLSTFSSEK AATPPATDDS AKAQRGSSVP EVSEGGRSAG SSPTRNLRTG SKQQSHPCSP VGAALPLQNC ELIENKL RK RLQGCWRKFL RECKDRDAGK RGEIAPADFL ALAEKFNLDI NRDECQQLIV KYDLKNDGTF AYCSFIQSCV LLLRAKES S LLQRMKIQNA NKMKEAGAET SSFYCALLRI QPRIAHCWRP MRRAFKAHDQ GGTGFLSVAD FRKVLRQYSV ILSEEEFFH VLEHYDKTLS AKIPYNDFLR AFLQ

分子 #7: Cilia- and flagella-associated protein 52

• 分子: 名称: Cilia- and flagella-associated protein 52 / タイプ: protein_or_peptide / ID: 7 / コピー数: 3 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 68.199891 KDa

配列

文字列:

MENQIQPKAE VAKLELEAVI GFNGHVPTGL KCHPDQEHLI YPLGCTILIQ AIKTQEQNFL HGHSNNVSCV TISKSGVYIA SGQVTFMGF KADIILWDYK KRELMARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKTEAIC GSPAAGLNVG N ATSVVFSQ CHDEMFVTAG NGTIRVWELD LPNRKIWPTE CQTGQMKRIV MSISMASDDS FFYLGTTTGD ILKMNPRTKL LA DTGPAKD KFSLGVSAIK CLKTGGLLVG SGAGLLVFCR SPSYKPIKKI QLQGGITSIS LRGEGHQFFV GTEESHIYRV NFT DFKETL VTTCHFEAVE DVVFPFGTAE LFATCAKKDI RVWHTLSNRE LLRITVPNMT CHGIDFMRDG KSLISAWDDG KIRA FAPET GRLMYVISNA HRIGVTAVAT TSDCTRVISG GGEGEVRVWQ IGHQTQKLEE ALKEHKSSVS CIRVKKNNEE CVTAS TDGT CIIWDLVRLR RNQMILANTL FQCVCYHPEE FQIITSGTDR KIAYWEVFDG SGIRELDGSL SGSINGMDIT PEGVHF VTG GNDHLVKVWD YNEGEVTHVG VGHSGNITRV RISPGNEYIV SVSADGAILR WKYPFPS

UniProtKB: Cilia- and flagella-associated protein 52

分子 #8: Cilia- and flagella-associated protein 45

• 分子: 名称: Cilia- and flagella-associated protein 45 / タイプ: protein_or_peptide / ID: 8 / コピー数: 4 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 65.621914 KDa

配列

文字列:

MPLSPAGVLS STSTASNRSR NRPRYRTKAL SSEVDESLFG GIKPLNQSDS PIVLLRDKHA IRKTLTALGL DHKPETIQLI TRDMVRELI IPTKDPSGES LIMSPEEFER IRWASHVPTR EELEAKEQAC KKEKEAIVDA VTTRKKIMKQ KEMVSRNNQK L SDLEEVAK ERAQNLLQRA SQLRMEQEEE LKDMKKIILN AKCHAIRDAQ ILEKQLIQKE LDAEEKRLDQ MMEVERQKSI QR QEELDKK RREERIRGRR HIVEQMEKNQ EERSLLAEQR EQEKEQMLEY MEQLQEEDLR DLERRHQQKL KMQAEIKRIN DEN QKQKAE LLAQEKLADQ MVMEFTKKKM AREAEFEAEQ ERIRREKEKE ISRLRALQEK VQDYQAEQDA LRAKRNQEVA DREW RRKEK ENAQKKMETE AQLRKSRLEQ VAFKEHSLAV QVQRDRDEFE RILRAQREQI EKEQQEEEKK AMERLQHANE LRRQV RENQ QKQVQDRIAI FEEGQRLKEE AQKRRERIEG IKKKKIEELR ATGLPEKYCI EAERKANIPT NTSVN

UniProtKB: Cilia- and flagella-associated protein 45

分子 #9: Cilia and flagella associated protein 210

• 分子: 名称: Cilia and flagella associated protein 210 / タイプ: protein_or_peptide / ID: 9 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 66.292844 KDa

配列

文字列:

MGTSEVVVRF GRRCGRAKEI TEIKNYEEDQ VLYPPLLPDK VDLRQVTIIP HNEWERIRDS LDSLTREAAH LRAKRKAKKE MHFRSQEVV KHWTNTYAGM RKQKLEAKKK RDEEIEAERQ ILDVEEAIYK QGERKKAIEY AKQYQFYQTE RVKNFHSGLL L SRVMKERD AQIEFQKSKI KSDKKWEEQL KLNIEKGFKE EQEKAEKQRR DRLALAKDHL KQIKEHEEEE VRRRKQEEKD AE EIKQQNL LYEIEMKKKL GMKKEKINES RRLFFEHLND KNVIKAIERQ QQEEEDEKIR KFIKAKKRLT QMGKEKEAET HRL MEERRE RINNFLSKLM KEKFDNEDLI IARDIAEAEA EWEKREREKH EKNKAELKAI AEHRAVVMNN KEEEERRRKI EAKE QLLAI LKADQIFWEH EKEKKRKADK ERREVQDAHI QQMAKNKFKV KQAKQAELED CRLTEALIAE KEKEFQDYAR EVIEL ESKS TNKYMHPLVK AVQEGPGGGH GPVFVDRGGL RPSYQVNDIT GVQLPFYNSQ GSKYNNFQKS KGRLGFTW

UniProtKB: Cilia and flagella associated protein 210

分子 #10: Cilia and flagella associated protein 276

• 分子: 名称: Cilia and flagella associated protein 276 / タイプ: protein_or_peptide / ID: 10 / コピー数: 3 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 19.304631 KDa

配列

文字列:

MPLTRDPFQH STLDNDDSYL GKIRASKKLP YKNPTHLAQQ QEPWCRLSST ATITSMRRDA CFFDPEIPKD DLDFRLSALY NHHSGTFKN KSEILIHQET IQDTHGIKIQ FPGESLPPPQ PPPITSQANI RHWINPKKES IHSIQGSIVS PHTAATNRGY S RKNDGGFF ST

UniProtKB: Cilia and flagella associated protein 276

分子 #11: Enkurin

• 分子: 名称: Enkurin / タイプ: protein_or_peptide / ID: 11 / コピー数: 3 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 29.949805 KDa

配列

文字列:

MDPTYSSESI YNLIPSDWKE PPQPPRYISI FKATVKDDMQ KFKTAMKTMG PAKLEVPSPK DFLKKHSKEK TLPPKKKFDR HEPKKPPVP LRTEHPVMGI QSEKNFINTN AANVIMGVAK KPKPVYVDKR TGDKHDLETS GLVPKYINKK DYGVTPEYIC K RNEEVKKA QEEYDNYIQE NLRKAAMKRL SDEEREAVLQ GLKKNWEEVH KEFQSLSVFI DSIPKKMRKQ KLEEEMKQLE HD IAVLEKH KIIYIANKK

UniProtKB: Enkurin

分子 #12: Parkin coregulated

• 分子: 名称: Parkin coregulated / タイプ: protein_or_peptide / ID: 12 / コピー数: 6 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 29.412234 KDa

配列

文字列:

MVAEKETLSL SKCPDKMPKR TKLLAQQPLP VHQPHPLVSE GFTVKAMMKN SVVRGPPTAG AFKERPTKPT AFRKFYERGD FPIALEHDS KGNKIAWKVE IEKLDYHHYL PLFFDGLCEM TFPYEFFARQ GIHDMLEHGG NKILPVIPQL IIPIKNALNL R NRQVICVT LKVLQHLVVS AEMVGEALVP YYRQILPILN IFKNMNVNSG DGIDYSQQKR ENIGDLIQET LEAFERYGGE DA FINIKYM VPTYESCLLN

UniProtKB: Parkin coregulated

分子 #13: Cilia- and flagella-associated protein 20

• 分子: 名称: Cilia- and flagella-associated protein 20 / タイプ: protein_or_peptide / ID: 13 / コピー数: 5 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 22.781389 KDa

配列

文字列:

MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

UniProtKB: Cilia-and flagella-associated protein 20

分子 #14: Protein Flattop

• 分子: 名称: Protein Flattop / タイプ: protein_or_peptide / ID: 14 / コピー数: 3 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 19.236705 KDa

配列

文字列:

MATNYSANQY EKAFSPRYLQ NWSLAKPTKQ RISSHEGYTQ IIANDRGHLL PSVPRSKASP WGSFMGTWDM PLKVPPARVT LTSRTAAGA ASLTKWIQKN PNLLKASNGL RPEILGKPHD PDGQRKFGKS IIKTVQQAPR STIIPSSPGS NLNSPDHPQS S HPSAGHTP GPQSPANSK

UniProtKB: Protein Flattop

分子 #15: Tektin

• 分子: 名称: Tektin / タイプ: protein_or_peptide / ID: 15 / コピー数: 4 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 48.432867 KDa

配列

文字列:

MAKLLQPPPK FLPSEWHIAN KNHYHRAEAQ RSRSERLVAE SQRLVDEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVSATE DLLIYQTRLE KALESLKEPL HITEKCLEYR EKRVGIDLVR DEVEQELIKE AEVIQGVRAL LTRTLEETAE Q IRLNRSAK YSLEKDLRDK FTALTIDDIC FSLNNNSPNI RYSENAVRIE PNSVSLEDWL DFSSTNVEKA DRQRNNSMTL KA LVDRILS QTASDLQRQC DVVDTAFRNG LKETKDARDK LAAHLAKVME EIASQEKNMT VLEKAILDQE GPAKVAHTRL ETR THRPNV ELCRDVAQYR LIKEVDEIAY NVARLKETLA QAQAELKGLN RRQLALQEEI QVKENSIYID EVLCVHMRRS IPPR DGDDR GQWAGGLRPD AVC

UniProtKB: Tektin

分子 #16: Tektin

• 分子: 名称: Tektin / タイプ: protein_or_peptide / ID: 16 / コピー数: 4 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 49.973676 KDa

配列

文字列:

MATLSVKPSQ RFRLPDWHTN SYLLSTNAER QRDASHQIRQ EARVLRNETN NQTIWDEHDN RTRLAERIDT VNRWKEMLDK CLTDLDAEI EALTQMKESA EQNLQAKNLP LDVAIECLTL RDCRRDIDVV KDPVEEELHK EVEVIEATKK ALQQKISQAF E KLCLLQEA RQQLNADHRG KMETLDIDRG CLSLNLKSPN ISLKINPTRV PSGSTTLQQW DDFSQFNKNR AEAEMKGAVE LR EAIALTI AETNNELEAQ RVATEFAFRK RLREMEKVYS ELKWQEKNTL EEIAELQEDI RHLEEDLRRK LQNLKLCHTR LET RTYRPN VELCRDQAQF GLTDEVHQLE ATIAALKQKL AQAQDALDAL YKHLARLQAD IACKANSMLL DTKCMDTRRK LTVP AEKFV PEVDTFTRTT NRTLSPLKTC QLELT

UniProtKB: Tektin

分子 #17: Tektin

• 分子: 名称: Tektin / タイプ: protein_or_peptide / ID: 17 / コピー数: 4 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 56.55284 KDa

配列

文字列:

MELLGSTLTA TYAHPRPTPT SLLPAISTMA SSYRDRFPHY NLTHSLSLPW RPSTYYKAAS NWPALDPYCT RAQSVSQSAM LPFVSNRTT LFTRYTPDDW YRSNLTNYLE SNTSRHNSER LRVDTSRLIQ DKYQQTRKTQ ADSTQNLGER VNDIGFWKSE I THELDAMI GETNELTDVK KKLERALMET EAPLQVAREC LFHREKRMGI DLVHDEVETE LLTEVDIILC CQERMKLHLD KA IAQLAAS RASQHELEKD LSDKQAAYRI DDKCHHLRNT SDGISYFRGV ERVDATVSVP ESWAKFTDDN ILRSQSERAA SVK LRDDIQ NLLVVTANEM WNQFNKVNLA FTNRIAETAD AKNKMQIHLA KTLQEIFQTE TTIDSIKKAI RDKAAFLKVA QTRL DERTR RPNIELCRDM AQLRLVNEVY EVDDTIQTLQ QRLRDAEDTL QSLVHTKATL EHDLAVKANS LYIDQEKCMT MRKSF PSTM RLVGFC

UniProtKB: Tektin

分子 #18: Tektin

• 分子: 名称: Tektin / タイプ: protein_or_peptide / ID: 18 / コピー数: 4 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 52.015508 KDa

配列

文字列:

MAQTDILTTK QPAPQTVLAC QLPRKLYDVA QNTGAHTSSG LATAGFRTAK YLADEWFQNC YARYHQAFAD RDQSEQQRHE SQQLVAETE ALAQRTQKDS MKKVGEHLQD THCWKSELQR HVEELVAETD LLLAQKQRLE RALDATALPF SIATDNLQCQ E RRQHPDLV RDWVEMELLK EAELIRNIQE LLKRTIMQAV NQIRLNREHK ETCEMGWSDK VEAYNIDEAC CRYHNQSTDV QF YPHSAKF EESASTPETW AKFTQDNLYG AERERLASVN LRELIECILQ DTAEDLRLQC DAVNLAFGRR CEELEDARHK LEY HLRKTL REITDQEHNV SVLKQAVKDK EAPLKVAQTR LYQRSHRPNV ELCRDTAQFR LVSEVEELNV SLATLREKLL EAEQ SLRNL EDTRMNLEKD LAVKTNSLFI NRQKCMTHRA CYPTVLQLAG YQ

UniProtKB: Tektin

分子 #19: RIB43A domain with coiled-coils 2

• 分子: 名称: RIB43A domain with coiled-coils 2 / タイプ: protein_or_peptide / ID: 19 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 45.003449 KDa

配列

文字列:

MEVAQPKDLE EDFGQAKKRH AELCRQKRIF NARNRIFGGD IDAWNVQVCD QKIREATEKA RQETFAAEMR QNDKITCILE DRARRDRKT LCKAISDFQQ SFQKPETRRE FDLSDPLALK KDLPARQSDN DARNTVSGMQ KFMGEDLNFH QRRKFQEEQN R EWSLQQQR EWKIAREDQK HAEDLYLETR LQFDETAKHL QNLEGTTRKA VCAAVKEFNK NQALESAEKK RQERKQEQED NL AEITNLL RGDLLSEDPQ QAASSFGPHR VVPDRWKGMT QEQLEQIRLV QKQQVQEKLR LQEEERQRDM DWDRQRVQKA RAT LLSERQ LQRQQRALRR ALDGSNLSLA KEQLLQKKYM KEIYTNQPTE DYFTQFNTRS R

UniProtKB: RIB43A domain with coiled-coils 2

分子 #20: RIB43A-like with coiled-coils protein 1

• 分子: 名称: RIB43A-like with coiled-coils protein 1 / タイプ: protein_or_peptide / ID: 20 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 44.590852 KDa

配列

文字列:

MFKTDLPPDP KEAAAIEARR NREKERQSRF LNVRTRVMGV DVEALNSQVE ERKLQEATEQ SKKAAYGTNQ VQYDVVAQML EKEQAERTR RLAKKVQEFR EQKQQLKNRS ELDLWDPHRL WKEFPPHLSN NDPYCGPASL QYFSGEDLNR STHLRMQQEQ F RYSLERQL QEQQQARIDY NCADMLNNQL RLAMDMRAAQ MAKLEESCRM AMMSAMANAN KFQVAEVAER QRREHQCQQE AN LTEIQNQ ITSDLLTENP QVAQNPVAPH RVLPYCWKGM TPEQRAAIRK VQEAQRHEKE AQRQAEQAMD SEWESQAERL ARA AQELEE QERELCAEFR RGLGSFNQQL AKEQKAQHNY LNSIIYTNQP TAQYHLQFNT SSR

UniProtKB: RIB43A-like with coiled-coils protein 1

分子 #21: EF-hand domain containing 1

• 分子: 名称: EF-hand domain containing 1 / タイプ: protein_or_peptide / ID: 21 / コピー数: 3 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 74.113344 KDa

配列

文字列:

MVSHPVHGLP FLPGTSFKDS TKTAFHRSQT LGYRNGYAVV RRPTVGIGGD RLQVNQLSQA ELDELANKAP VLTYGQLKRA PPAEFIPAH VAFDKKVLKF DAYFQEDVPG STEEHYRIRQ VNIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRLPKNDR G DHYHWKDL NRGINITIYG RTFRIVNCDK FTQVFLESQG IELNPPEKMA LDPYTELRKQ PLRKYVTPTD FDQLKQFLTF DK QVLRFYA IWDDTDSMFG ECRTYIIHYY LMDDTVEILE VHERNDGRDP FPLLMNRQRM PKVLVENAKN FPQCVLEISD QEV LEWYTA KDFIVGKPLT MLGRTFFIYD CDPFTRQYYK EKFGISDLPR IDVSKKEPPP VKQELPPYNG YGLIEDSAQN CLAL IPKAP KKDVIKMLMN DNKVLRYLAA LESPIPEDKG RRFVLSYFLA TDMISIFEPP VRNSGIIGGK YLGRTKVVKP DSSME NPIY YGPSDFFIGA VIEVFGHRFV ILDSDDYVLK YMESNPAQFS PEALLSIQNH IRRRDTPALE LDSQRTEKDP GTQELE VLI DTIQKQLKDH PCRDSIREAF RIYDTEASGY VDRETFFKIC DSLHLPVDDS LIKELVRMCS HGDDKMDYSS FVRAFSH

UniProtKB: EF-hand domain-containing protein 1

分子 #22: EF-hand domain-containing family member C2

• 分子: 名称: EF-hand domain-containing family member C2 / タイプ: protein_or_peptide / ID: 22 / コピー数: 4 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 87.254148 KDa

配列

文字列:

MALPLLPGNS FNRNIGKEKF HKSQHWGFCN NVRMLVSEDK PGIGGELLLG QKMKPKYSVF PKEMGTGLPS WVAFDKQVLS FDAYLEDEV PDKSQENYRI RCYKIYFYLE DDTIQVNEPA LKNSGLPKGI FIRRHRISLP PPDEDQFYTV YHFNINVDIV L YGRTFKIY DCDTFTKNFL KKIGVKLNPP GQCPEDPYMK TRREKLDCVE PLRPYESFDT LKQFLEYDRK VLRFFCLWDD SG SLFGDRR ELILHYFLSD DTIEIKEVMP HNSGRDAMSL FLQRRKLPKY GPPGVYQPGQ IADRTVLNVY GGLSRRRVYS YLL DKYKLG NLDQEFYKDS DLSIGTTINV WGRKVLLCDC DEFTKTYYKT KYGIENFTSI SCKAPPPPKI ERKFPPYTGF GSEE DSLRS CIGLVPTPHR RNFKKFMEKD SYGNISNTLR FFAKLITHKC ADVERMFVIS YFLSDDTISV FEPIERNSGY TGGMF LKRT RVKKPGQEVF KSEFSEYVKA EELYVGAKVD VNGYLFLLLN ADEYTLNYME KNSDKFPRSN IKLALQKVKE EESKSR ELK QVFTAADCKH TKMVDYNTFR EIMMNLTVGK LTDQELITIA RHYRVPEEPC PDTCIVMAQA HEQLKKHAFE NFERLIA AC VYQDREKKKV LPSKDIKRLC KSSRLPFNDD LLGALLSKFE DSEKQINYES FFCALNWRAN PMPELEAVSY LKERCEDE W LGMPSPIPVK CISYLNLLKD VFGLEEE

UniProtKB: EF-hand domain-containing family member C2

分子 #23: Ciliary microtubule inner protein 2B

• 分子: 名称: Ciliary microtubule inner protein 2B / タイプ: protein_or_peptide / ID: 23 / コピー数: 5 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 30.596564 KDa

配列

文字列:

MTMASTFIPG LNPKNPHYIP GYTGHCPLLR FSMGQTYGQT TGQLLRGSPG LAWPPAHRTL LPPIRSPRSP EIPRRSLPVR RGHERLSSS MIPGYTGFVP QAQFIFAKNC NQVWAEALND FTQWYGGQGS QELPKEAKGE KDMEKDQQPK LEAEVEAENE P ELGQEVEQ ASPYSMDDRD PRKFFMSGFT GYVPRARFLF GSSFPVLSNQ ALQEFDQMKS QGRSQKDPKH LPPLSRTYPH NL GLLPKYG GYVPGYKFQF GRTYGHLTHD ALGAL

UniProtKB: Ciliary microtubule inner protein 2B

分子 #24: Ciliary microtubule inner protein 2B

• 分子: 名称: Ciliary microtubule inner protein 2B / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 28.407986 KDa

配列

文字列:

RFSMGQTYGQ TTGQLLRGSP GPAWPPAHRT LLPPIRSPRS PEIPRRSLPV RRGHERLSSS MIPGYTGFVP QAQFIFAKNC NQVWAEALN DFTQWYGGQG SQELPKEAKG EKDMEKDQQP KLEAEVEAEN EPELGQEVEQ ASPYSMDDRD PRKFFMSGFT G YVPRARFL FGSSFPVLSN QALQEFDQMK SQGRSQKDPK HLPPLSRTYP HNLGLLPKYG GYVPGYKFQF GRTYGHLTHD AL GALSTLQ KQLLA

UniProtKB: Ciliary microtubule inner protein 2B

分子 #25: Sperm-associated antigen 8 isoform 1

• 分子: 名称: Sperm-associated antigen 8 isoform 1 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 50.53725 KDa

配列

文字列:

MVCRLYSNAV PSSECRKMET TDSTELSPSR SLDEQPSSEE LGTTSDPLPS LDGRHRSALA AATAAASAAA AATAAASTAK AAALSTQTP APYSERSLLL EPFSDNLTAA HHTTPRFTHK IGHGRLGFEP VQVSHIARNS YTANDPISSQ GPVPGSSSDP V PGSSSGPV PGSSSSPGPG TSSGPGGGSG PGPGGRSGEG TGPGPASDSR HSPGCGHDPG FSSSAPPGFR NPRADLVPNY AS WSHDSHW EPQKQPWKFL QVSEPGTRGH WKPSEVEGKS KIPSETLPRG QCLLYNWEEE RATNHLDQVP SMQDGSESFF FRH GHQGLL TLWPQSPMSS RTTQKDSYQP PGNHSQPVRG KREAMLEMFL RHQICKEVQA EQEATRKDSE VESVTHHDYR KELV HAGPP TPTRPHDYRT EQPETFWIQR APQLPGVSNI RTLDTPFRKN CSFSTPVPLS LGQPLPYEPE DYSH

UniProtKB: Sperm-associated antigen 8 isoform 1

分子 #26: Nucleoside diphosphate kinase homolog 7

• 分子: 名称: Nucleoside diphosphate kinase homolog 7 / タイプ: protein_or_peptide / ID: 26 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 42.532766 KDa

配列

文字列:

MNHSERFVFI AEWYDPNASL FRRYELLFYP GDGSVEMHDV KNHRTFLKRT KYEDLHLEDL FIGNKVNVFS RQLVLIDYGD QYTARQLGS KKEKTLALIK PDAVSKAGEI IEIINKAGFT LTKLKMMTLS RKEATDFHID HQSRPFLNEL IQFITSGPII A MEILRDDA ICEWKKLLGP ANSGLARTDA PGSIRAVFGT DGIRNAAHGP DSLSCAAREM ELFFPSSGVC GPANTAKFTN CT TCCIVKP HAISEGLLGK ILMAIRDAGF EISAMQMFNM DRVNVEEFYE VYKGVVSEYN EMVTEMYSGP CVAMEIQQTN PTM TFREFC GPADPEIARH LRPGTLRAIF GKTKIQNAVH CTDLPEDGLL EVQYFFKILD N

UniProtKB: Nucleoside diphosphate kinase homolog 7

分子 #27: Cilia and flagella associated protein 161

• 分子: 名称: Cilia and flagella associated protein 161 / タイプ: protein_or_peptide / ID: 27 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 35.381199 KDa

配列

文字列:

MAKNLYGPGV RMGNWNEDVY LEEERMRDFL AKRDEGQLLI QKNRRLKENL LRPMQLSVSE DGYIHYGDKV MLVNPDHPEV EADLFLGGD LSLCMTPDEI KAYLSDELEV PCGLSAAQTK IPVGRNTFTI LCAAGEVIGQ VVRYGQNFRL GIAGGFEGRM F YLSSDHRT LMKSSKRSWL QEVYLTDEDS YLNCWQAAFP DPQLRLEYEG FPIPANAKIL INHCHTNRGL VAHRHLFLST YF GKEVEVA AHTYLDSHRV EKPKNHWMLV TGNPRKNAPT VLDLPKPPAE GTRALEQVAE TPGSSTTPGA HACSHPL

UniProtKB: Cilia and flagella associated protein 161

分子 #28: Chromosome 1 C9orf135 homolog

• 分子: 名称: Chromosome 1 C9orf135 homolog / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 26.817912 KDa

配列

文字列:

MESSDSSCRD WCSGRQYRFE LGPTDLTERK GSLTLRSHHK KYSKPVLVYS WNHDREAYPK DYDIEGPEEV KKLCNSTYCR LGTHEPTVW KSETHEKMAQ VYLNTEFTEI KSKALLNEET ISSGIIERDT GLPATGFGAL FTKHPPDWRK MYTLTTYAEE Y APPYQYQP FDYPCQDDDY SVVHRKCRSQ FTDLNGSKRF GINTWHDESG IYANSYLKRK LYSLTTSPIA PF

UniProtKB: Cilia and flagella associated protein 95

分子 #29: Piercer of microtubule wall 1

• 分子: 名称: Piercer of microtubule wall 1 / タイプ: protein_or_peptide / ID: 29 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 15.692243 KDa

配列

文字列:

MSEENPQECA EPEEPEATAS PEKTSDYYRV SEDLPARFNN PAWFRGYRTK EPPSVYRTSN QAYGSRAPTV HEMPKVFYPN SHKFSRQLA AGGMFQNNTF NVYMEKSIVT GPDNYITSYD RLNFHPSYRV NKPSICD

UniProtKB: Piercer of microtubule wall 1

分子 #30: Chromosome 1 C15orf65 homolog

• 分子: 名称: Chromosome 1 C15orf65 homolog / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 13.888583 KDa

配列

文字列:

MTECDWDKKS TSASNSDTEM KPEQRPSCVN PGNPVFSCML DPRSLHTATS LSKPKMIMYK TNSSNYGEFL PMPQFFPCYY TPKEQVFSG HIRATGFYQN NTLNTTPDRT RTLDFPNFQH TL

UniProtKB: Piercer of microtubule wall 2

分子 #31: EF-hand domain family member B

• 分子: 名称: EF-hand domain family member B / タイプ: protein_or_peptide / ID: 31 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 98.40218 KDa

配列

文字列:

MEMCSFVRVP SPKVLGSSKA LGSSLQPGTA YPLEMSALGK VRVSERKSPL GMAVGFVQEG KGGLGERRVF MGTTSPTELG LRVRLGKED SSCTNKYEER IASSETKSPS SKGLEMRLKR PDISRTFMDG SNLGVNRVSA LQETKPSLSM LPGRVGLENE R FLAGCCPG RVKRPPLGMV CKSPQAVGSR RCPVAGDLEG LGASMGKQLV LDMEPRQELE KESTCVVMKP KAEMEPPVEV DM GLPQPED PDETNPTDLH MGLVIEPSEC QLAQLHEEQK VAENTELGVE PPDHIRPIYS GKFFDRTPCW PSAGKVLPIG YRV ATCLTE KLPRLMTPPE AKKFFNFRYP PAGAERVFYG RANDPQIAPY LTHGIRSKIS IPAKVLINPQ PITTFQQKIK DKKE SVYFS NQRAPLGKSH DQTPGLPKGL DVPNTTFGTA IIRELSLGDV VNPPKPYEEV FEEGQAGHDL YVVSHNDYFV GEAKN RKYN PSSFHRFNLY GVPTPHTNDG RNMAKTLHWL HELQMKRGAK IESKRADDFK EKFQHKLGRV LDPIAETMTV PPDHTF GAC LRPDEYGAGD LIHRRLPGEY LRGKDRERAL IAATRHHLKK VNYQNFDTLL AAFRHYDKKG DGAIDKAELQ EACNQAS LH LDEKLLDQLF EYCDVDNDGL INYLEFANFL NWKDKTPLKG YEERIIIKGR KPDCANPVET NAEESEPAFL IKPEDIIL K EPGSSEKTLR TLLRPSDKVS NYYKTTSSEI GADVGAVLST CHPIFGVPTI RSDIPAPRIR RISDRTNYGE DGNAYSLLY PTIFGQKGVF ERDFFKTRSK KEIAEILCNI GVNLSEDEFE NVWNLASKKH HRGEVCVENI RNVLDELQHA ERTKCKTAV

UniProtKB: EF-hand domain family member B

分子 #32: Cilia and flagella associated protein 141

• 分子: 名称: Cilia and flagella associated protein 141 / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 12.197253 KDa

配列

文字列:

MSMEKMVKVE ESFQRAVGLK KMVDRWQNSH THCLWQMTLS QRRNPYAILR MQNTVVQELA LANKQLLMVR QAALHQLFEK EHRQYQQEL NQMGKAFYVE RL

UniProtKB: Cilia and flagella associated protein 141

分子 #33: Meiosis-specific nuclear structural protein 1

• 分子: 名称: Meiosis-specific nuclear structural protein 1 / タイプ: protein_or_peptide / ID: 33 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 60.768789 KDa

配列

文字列:

MASKRRTLSF SERHQKLVDA NYCKKLHVEA LKKLQNQVRN QMVRNENDDR AEHKRFLRLL QNEQFELDME EAIQKAEENR RLRECQLEQ EEKLALELAK LKHESLKDEK MRQQVRENSI ELRELEKKLQ AAYMNKERAA QIAEKDAIKY EQMKRDAEIA K TMMEEHER LIKEENATED KRNKVKAQYN LDLEKQLEEQ EKKKQEAYEQ LLKEKLMIDE IVRKIYEEDQ LERQQKLEKM KT TQRYIEE FKKQQAVWRK KKREEMEEEN RRIIEFAKMQ QQREEDWMAK VQETEEKRLQ LQNTLSQRLE EMLRQREDLE QVR QELYLE EQAEIHKKKL EEEAEKKLRK QKELKQNFME QMALKELVLQ AAKEEEEIFR KAMLAKYAED DRIELMNAQK QRMK QLEHK KAVEKLIEER RNQFLADKQR ELEEWQLQQR RQGCVNEIIE EERLKLLKEH ATKLLGYLPK GVFKKVDDID MLGEE FRKA YQKRSEICEH K

UniProtKB: Meiosis-specific nuclear structural protein 1

分子 #34: Cilia- and flagella-associated protein 53

• 分子: 名称: Cilia- and flagella-associated protein 53 / タイプ: protein_or_peptide / ID: 34 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 62.200133 KDa

配列

文字列:

MYSQRFGIVQ REVKGPTPKV VIMRAKPPKG QGAEQHLERI RHSHQKHHVI LASTKSNERD RLKAEWDQHN DRKFVDSLVK ARIKDAMQG FIINTEERRN KLRELLASEE NEYFTEMRLK EETIEEKKDR MKDKTRLLRE KKEKERQDFV AEKLDQQFRE C CEELRVEL FCIHQKAVCE ERKAQIAFNE ELKRQKLVEE QMFLKLWEED RLAKEKREVE EARRQKELVE NTRLGLNAQI TS IQAQRQA ARQLKEEEAR LVENNKAQVK LENEQDKLKK QKTKQEIRAV LQKALQEKME QIQQEYREEQ DLNMKLTQNA LQN LQEEAD KKKQKREDMI REQKIYHQYL AQRHEEEKAQ EKELDRILEK EKEKKFAEKD KELRLQKEAR KQLVNEVMCT RKLQ VQEKL QRKAKEQEER AMEQERINEG LKELNREEQE NFARRSSLAQ EYRKQLQMQI CSRQQAREAE KEEERREFEA GIAAE KNFQ DKIEKILSTH QVLPRNIHPM RRACPSKLPP

UniProtKB: Cilia- and flagella-associated protein 53

分子 #35: CFAP107/C1orf158

• 分子: 名称: CFAP107/C1orf158 / タイプ: protein_or_peptide / ID: 35 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 23.449852 KDa

配列

文字列:

MQYFMAVNPQ SFSTPSWKVE TKYSTRVLTG NWVEERRKFT KATEK(UNK)PQSI YRKEYVPFPG HRPDQISRWY SKRRVE GLP YKHLITHHQE PSHHHLISTY DDHYNRHNYN PGLPLLRTWN KHKLLWLPEK ADFPLLAPPT NYGLYERLKQ RWLPPPE AT LRESMYTCSY PRPPSGAMSR REHAIPVPPP RLQPVPRF

UniProtKB: Cilia and flagella associated protein 107

分子 #36: Cilia and flagella associated protein 77

• 分子: 名称: Cilia and flagella associated protein 77 / タイプ: protein_or_peptide / ID: 36 / コピー数: 4 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 32.726729 KDa

配列

文字列:

MPEASSSQDL MRWKKKPPVR RTVSQICPPP RRPLTVADIR PGMENDRLGV VRDSMFQNPL IVKAELGKPR ERSSSLPGID FNYGLYIRG LDGGVPEAIG HWNVFKQQPT CPHELTRNYI VMNRGAVKAG LVTARENFNY HQLKDIRISD QDDRRLKREP P SLPPNMTF GIRARPSTPF FDLLQHRYQQ LWVQEQKATQ KAIKLEKKHK VTLGPLYETR SSQLRKYKPP VRLDALWHMP HF QKVGPHL DTFPTEADRQ RAFKAHREEC AVRQGTLRMG NYTHP

UniProtKB: Cilia and flagella associated protein 77

分子 #37: Cilia and flagella associated protein 144

• 分子: 名称: Cilia and flagella associated protein 144 / タイプ: protein_or_peptide / ID: 37 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 16.325543 KDa

配列

文字列:

MAGRQKEKVI RDEVHQNQIL RELYLKELRT QILYTQYHVN PLRKVHTIAR KPMSWHDNLE EPEDARFLNL IHHAAQGPRK KYPETQTES QEIGWDSEPL VNPARDDRRL NHFRVYNDIT LYKAKMWSLG EKDRHK

UniProtKB: Cilia and flagella associated protein 144

分子 #38: Cilia-and flagella-associated protein 96

• 分子: 名称: Cilia-and flagella-associated protein 96 / タイプ: protein_or_peptide / ID: 38 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 34.594367 KDa

配列

文字列:

MPGEGGKTDM ERIGLFSEME YVTVGDKYVS QFNRSFNEAA SKNRQMLPGG SKEMSSLQAG YFDPHFVRIF EGEGYVNLNQ VRRRHMMEE AKKNLGKAFL PSSGDKKPCG LGSYYGTIGG PVPFFSAQSK PKEKYEPPGK NLYTNPGKKG TGYGYANITI G NQFSHSTD FYDAAKLNYK KENEEHRRLL KGTAFKLNLY PREYFDANPY FFEKPLPPIK KAEKKETLAN PFKPSSPGKK AG GMKAGTF DPYPSHSADP YVVKLTSPIS SKGAKIFHPP GGPKSRPIES VMTLNVKRAL NMKNYKTASV QSY

UniProtKB: Cilia-and flagella-associated protein 96

分子 #39: Sperm acrosome associated 9

• 分子: 名称: Sperm acrosome associated 9 / タイプ: protein_or_peptide / ID: 39 / コピー数: 7 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 25.335152 KDa

配列

文字列:

MTMNEVKESL RSVEQKYRLF QQQQFTFIAA LEHCREDAHD KIRPISSIGQ VQSYMEHHCH NATDRRILLM FLDICTELSK LCQHFEALH AGTPVTNNLL EKCKSLVSHS NDLSSLRAKY PHDVVNHLSC DEARNHYGGV VSLIPVVLDL MKEWVAHSEK L PRKALQHV SEPQGAPQAA AHAPCGTQPW LRKHKCWQLT RDGLKPGAGD KRCSKPPWRP PGGKL

UniProtKB: Sperm acrosome associated 9

分子 #40: Tubulin alpha chain

• 分子: 名称: Tubulin alpha chain / タイプ: protein_or_peptide / ID: 40 / コピー数: 136 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 49.797035 KDa

配列

文字列:

QRECISVHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKELIDLVL DRIRKLADQC TGLQGFLIFH SFGGGTGSGF TSLLMERLSV D YGKKSKLE FSIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLISQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCL LYR GDVVPK DVNAAIAAIK TKRSIQFVDW CPTGFKVGIN YQPPTVVPGD LAKVQRAVCM LSNTTAIAEA WARLDHKFDL MYAK RAFVH WYVGEGMEEG EFSEAREDMA ALEKDYEEVG MDSVEGEGEE EE

UniProtKB: Tubulin alpha chain

分子 #41: Tubulin beta chain

• 分子: 名称: Tubulin beta chain / タイプ: protein_or_peptide / ID: 41 / コピー数: 133 / 光学異性体: LEVO
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 49.798211 KDa

配列

文字列:

MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLGNPVVTR GACLWLGGGE GPQPPSPLRS GLSP

UniProtKB: Tubulin beta chain

分子 #42: GUANOSINE-5'-TRIPHOSPHATE

• 分子: 名称: GUANOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 42 / コピー数: 136 / 式: GTP
• 由来(天然): 生物種: Sus scrofa (ブタ)
• 分子量: 理論値: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, エネルギー貯蔵分子*YM

分子 #43: GUANOSINE-5'-DIPHOSPHATE

• 分子: 名称: GUANOSINE-5'-DIPHOSPHATE / タイプ: ligand / ID: 43 / コピー数: 133 / 式: GDP
• 分子量: 理論値: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, エネルギー貯蔵分子*YM

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: filament

試料調製

• 緩衝液: pH: 7.4
• 凍結: 凍結剤: ETHANE

電子顕微鏡法

• 顕微鏡: TFS KRIOS
• 撮影: フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k); 平均電子線量: 50.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 2.5 µm / 最小 デフォーカス（公称値）: 0.5 µm
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

初期モデル

モデルのタイプ: EMDB MAP
EMDB ID:

40619

• 最終 再構成: 解像度のタイプ: BY AUTHOR / 解像度: 3.65 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 113809
• 初期 角度割当: タイプ: MAXIMUM LIKELIHOOD
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD