+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | WT 12C IM fraction, B-b5 | |||||||||
![]() | WT 12C IM fraction, B-b5 | |||||||||
![]() |
| |||||||||
![]() | 50S subunit / assembly intermediate / RNA-protein complex / ribosome | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 5.76 Å | |||||||||
![]() | Lee J / Sheng K / Williamson JR | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: 50S ribosome assembly intermediates at low temperature reveal bound RluB Authors: Lee J / Sheng K / Williamson JR / Gebert L | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 136.5 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 14.8 KB 14.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11.2 KB | Display | ![]() |
Images | ![]() | 79.9 KB | ||
Filedesc metadata | ![]() | 4.3 KB | ||
Others | ![]() ![]() | 134.5 MB 134.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | WT 12C IM fraction, B-b5 | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.15 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: WT 12C IM fraction, B-b5, half map B
File | emd_44804_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | WT 12C IM fraction, B-b5, half map B | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: WT 12C IM fraction, B-b5, half map A
File | emd_44804_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | WT 12C IM fraction, B-b5, half map A | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : WT 12C 50S ribosomal subunit assembly intermediate
Entire | Name: WT 12C 50S ribosomal subunit assembly intermediate |
---|---|
Components |
|
-Supramolecule #1: WT 12C 50S ribosomal subunit assembly intermediate
Supramolecule | Name: WT 12C 50S ribosomal subunit assembly intermediate / type: complex / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 Component:
Details: Most of the sucrose was removed by spin concentration. | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 | ||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV / Details: 3 microliter of the sample was added.. |
-
Electron microscopy
Microscope | FEI TALOS ARCTICA |
---|---|
Details | In order to account for highly preferred orientation of the specimen, data were acquired using tilts at -20 degrees. |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Average exposure time: 5.0 sec. / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 36000 |
Sample stage | Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |