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- EMDB-42742: Constituent EM map: Focused refinement BSol2 of the Structure of ... -
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Open data
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Basic information
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Title | Constituent EM map: Focused refinement BSol2 of the Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium | |||||||||
![]() | Constituent EM map: Focused refinement BSol2 of the Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium | |||||||||
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![]() | calcium channel / MEMBRANE PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.61 Å | |||||||||
![]() | Miotto MC / Marks AR | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for ryanodine receptor type 2 leak in heart failure and arrhythmogenic disorders Authors: Miotto MC | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 243.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.2 KB 16.2 KB | Display Display | ![]() |
Images | ![]() | 7.2 KB | ||
Filedesc metadata | ![]() | 4.4 KB | ||
Others | ![]() ![]() | 473.6 MB 473.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8uq2C ![]() 8uq3C ![]() 8uq4C ![]() 8uq5C ![]() 8uxcC ![]() 8uxeC ![]() 8uxfC ![]() 8uxgC ![]() 8uxhC ![]() 8uxiC ![]() 8uxlC ![]() 8uxmC C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Constituent EM map: Focused refinement BSol2 of the Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_42742_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_42742_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Complex of RyR2-R420W and Calstabin-2
Entire | Name: Complex of RyR2-R420W and Calstabin-2 |
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Components |
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-Supramolecule #1: Complex of RyR2-R420W and Calstabin-2
Supramolecule | Name: Complex of RyR2-R420W and Calstabin-2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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-Supramolecule #2: Ryanodine Receptor 2
Supramolecule | Name: Ryanodine Receptor 2 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Supramolecule #3: Peptidyl- cis-trans isomerase FKBP1B
Supramolecule | Name: Peptidyl- cis-trans isomerase FKBP1B / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 2.5 mg/mL | |||||||||||||||||||||||||||
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Buffer | pH: 7.4 Component:
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Temperature | Min: 80.0 K / Max: 100.0 K |
Specialist optics | Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Average electron dose: 58.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.5 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: INSILICO MODEL / In silico model: CryoSPARC ab initio |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.61 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 597792 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
-Atomic model buiding 1
Initial model | PDB ID: Chain - Source name: PDB / Chain - Initial model type: experimental model |
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