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- EMDB-4269: Abp1-SNAP bound to Arp2/3 complex (class 1) -

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Basic information

Database: EMDB / ID: 4269
TitleAbp1-SNAP bound to Arp2/3 complex (class 1)
Map dataAbp1-SNAP bound to Arp2/3 complex (class 1)
SampleArp2/3 complex with bound Abp1 dimer:
SourceSaccharomyces cerevisiae (baker's yeast)
Methodsingle particle reconstruction / 21 Å resolution
AuthorsSokolova OS / Goode BL
CitationJournal: To Be Published
Title: Abp1 mechanism in controlling Arp2/3 complex-dependent actin nucleation and branch stability
Authors: Guo S / Sokolova OS / Chung J / Padrick S / Gelles J / Goode BL
DateDeposition: Jan 23, 2018 / Header (metadata) release: Mar 14, 2018 / Map release: Nov 28, 2018 / Last update: Nov 28, 2018

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 0.18
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.18
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
Supplemental images

Downloads & links


Fileemd_4269.map.gz (map file in CCP4 format, 4001 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
100 pix
2.5 Å/pix.
= 250. Å
100 pix
2.5 Å/pix.
= 250. Å
100 pix
2.5 Å/pix.
= 250. Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.5 Å
Contour Level:0.18 (by author), 0.18 (movie #1):
Minimum - Maximum-0.44605464 - 1.5727929
Average (Standard dev.)0.021142097 (0.11353068)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 250.0 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.52.52.5
M x/y/z100100100
origin x/y/z0.0000.0000.000
length x/y/z250.000250.000250.000
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS-50-50-50
D min/max/mean-0.4461.5730.021

Supplemental data

Sample components

Entire Arp2/3 complex with bound Abp1 dimer

EntireName: Arp2/3 complex with bound Abp1 dimer / Number of components: 1

Component #1: cellular-component, Arp2/3 complex with bound Abp1 dimer

Cellular-componentName: Arp2/3 complex with bound Abp1 dimer / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)

Experimental details

Sample preparation

SpecimenSpecimen state: particle
Sample solutionpH: 7.5
VitrificationCryogen name: NONE

Electron microscopy imaging

ImagingMicroscope: JEOL 2100
Electron gunElectron source: LAB6 / Accelerating voltage: 200 kV / Electron dose: 13 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN ULTRASCAN 1000 (2k x 2k)

Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 2500
3D reconstructionResolution: 21 Å / Resolution method: FSC 0.5 CUT-OFF

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