|Entry||Database: EMDB / ID: EMD-4268|
|Title||Abp1-SNAP bound to Arp2/3 complex (class 2)|
|Sample||Arp2/3 complex with bound Abp1 dimer|
|Biological species||Saccharomyces cerevisiae (baker's yeast)|
|Method||single particle reconstruction / Resolution: 21 Å|
|Authors||Sokolova OS / Goode BL|
|Funding support|| United States, 1 items |
|Citation||Journal: Nat Commun / Year: 2018|
Title: Abp1 promotes Arp2/3 complex-dependent actin nucleation and stabilizes branch junctions by antagonizing GMF.
Authors: Siyang Guo / Olga S Sokolova / Johnson Chung / Shae Padrick / Jeff Gelles / Bruce L Goode /
Abstract: Formation and turnover of branched actin networks underlies cell migration and other essential force-driven processes. Type I nucleation-promoting factors (NPFs) such as WASP recruit actin monomers ...Formation and turnover of branched actin networks underlies cell migration and other essential force-driven processes. Type I nucleation-promoting factors (NPFs) such as WASP recruit actin monomers to Arp2/3 complex to stimulate nucleation. In contrast, mechanisms of type II NPFs such as Abp1 (also known as HIP55 and Drebrin-like protein) are less well understood. Here, we use single-molecule analysis to investigate yeast Abp1 effects on Arp2/3 complex, and find that Abp1 strongly enhances Arp2/3-dependent branch nucleation by stabilizing Arp2/3 on sides of mother filaments. Abp1 binds dynamically to filament sides, with sub-second lifetimes, yet associates stably with branch junctions. Further, we uncover a role for Abp1 in protecting filament junctions from GMF-induced debranching by competing with GMF for Arp2/3 binding. These data, combined with EM structures of Abp1 dimers bound to Arp2/3 complex in two different conformations, expand our mechanistic understanding of type II NPFs.
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_4268.map.gz / Format: CCP4 / Size: 3.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 2.5 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Entire Arp2/3 complex with bound Abp1 dimer
|Entire||Name: Arp2/3 complex with bound Abp1 dimer / Number of components: 1|
-Component #1: cellular-component, Arp2/3 complex with bound Abp1 dimer
|Cellular-component||Name: Arp2/3 complex with bound Abp1 dimer / Recombinant expression: No|
|Source||Species: Saccharomyces cerevisiae (baker's yeast)|
|Specimen||Specimen state: Particle|
|Sample solution||pH: 7.5|
|Vitrification||Cryogen name: NONE|
-Electron microscopy imaging
|Imaging||Microscope: JEOL 2100|
|Electron gun||Electron source: LAB6 / Accelerating voltage: 200 kV / Electron dose: 13 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN ULTRASCAN 1000 (2k x 2k)|
|Processing||Method: single particle reconstruction / Number of projections: 3500|
|3D reconstruction||Resolution: 21 Å / Resolution method: FSC 0.5 CUT-OFF|
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