EMDB-4255: human Bact spliceosome core structure

Basic information

• Entry: Database: EMDB / ID: EMD-4255
• Title: human Bact spliceosome core structure

Sample

• Complex: human Bact spliceosome state 1 unmasked
  • Protein or peptide: x 24 types
  • RNA: x 3 types
• RNA: x 1 types
• Ligand: x 4 types

• Keywords: spliceosome / human / HELA / BACT / dynamics / SPLICING

Function / homology

Function:

RES complex / negative regulation of chemokine-mediated signaling pathway / snoRNA splicing / U11/U12 snRNP / regulation of retinoic acid receptor signaling pathway / post-mRNA release spliceosomal complex / regulation of vitamin D receptor signaling pathway / B-WICH complex / splicing factor binding / nuclear retinoic acid receptor binding / embryonic brain development / U12-type spliceosomal complex / RNA splicing, via transesterification reactions / mRNA 3'-end processing / blastocyst formation / regulation of mRNA splicing, via spliceosome / C2H2 zinc finger domain binding / U2-type catalytic step 1 spliceosome / positive regulation of mRNA splicing, via spliceosome / pre-mRNA binding / positive regulation by host of viral transcription / U2-type precatalytic spliceosome / U2-type spliceosomal complex / positive regulation of vitamin D receptor signaling pathway / mRNA cis splicing, via spliceosome / U2-type prespliceosome assembly / Transport of Mature mRNA derived from an Intron-Containing Transcript / nuclear vitamin D receptor binding / Notch binding / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U2-type catalytic step 2 spliceosome / RUNX3 regulates NOTCH signaling / NOTCH4 Intracellular Domain Regulates Transcription / SAGA complex / RHOBTB1 GTPase cycle / U2 snRNP / RNA Polymerase II Transcription Termination / NOTCH3 Intracellular Domain Regulates Transcription / U2-type prespliceosome / WD40-repeat domain binding / protein peptidyl-prolyl isomerization / positive regulation of transcription by RNA polymerase III / positive regulation of neurogenesis / nuclear androgen receptor binding / K63-linked polyubiquitin modification-dependent protein binding / precatalytic spliceosome / Notch-HLH transcription pathway / Formation of paraxial mesoderm / positive regulation of transforming growth factor beta receptor signaling pathway / SMAD binding / positive regulation of transcription by RNA polymerase I / spliceosomal complex assembly / regulation of RNA splicing / mRNA Splicing - Minor Pathway / mRNA 3'-splice site recognition / spliceosomal tri-snRNP complex assembly / Prp19 complex / U5 snRNA binding / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / protein localization to nucleus / U5 snRNP / positive regulation of G1/S transition of mitotic cell cycle / U2 snRNA binding / U6 snRNA binding / pre-mRNA intronic binding / regulation of DNA repair / Cajal body / RHOBTB2 GTPase cycle / retinoic acid receptor signaling pathway / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / cellular response to retinoic acid / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / positive regulation of RNA splicing / DNA damage checkpoint signaling / peptidyl-prolyl cis-trans isomerase activity / positive regulation of protein export from nucleus / nuclear receptor binding / RNA polymerase II transcription regulatory region sequence-specific DNA binding / RNA polymerase II CTD heptapeptide repeat P3 isomerase activity / RNA polymerase II CTD heptapeptide repeat P6 isomerase activity / peptidylprolyl isomerase / stem cell differentiation / spliceosomal complex / Downregulation of SMAD2/3:SMAD4 transcriptional activity / response to cocaine / RING-type E3 ubiquitin transferase / negative regulation of protein catabolic process / mRNA splicing, via spliceosome / positive regulation of neuron projection development / B-WICH complex positively regulates rRNA expression / Pre-NOTCH Transcription and Translation / NOTCH1 Intracellular Domain Regulates Transcription / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / positive regulation of protein import into nucleus / nuclear matrix / fibrillar center

Domain/homology:

: / Pre-mRNA-splicing factor CWC24-like / Ist3-like, RNA recognition motif / : / Bud13 / : / Pre-mRNA-splicing factor of RES complex / : / SF3B6, RNA recognition motif / PWI domain superfamily / PWI domain / PWI domain profile. / Cactus-binding C-terminus of cactin protein / PWI domain / PWI, domain in splicing factors / : / SF3A2 domain / : / Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11) / mRNA splicing factor Cwf21 domain / cwf21 domain / Splicing factor 3B, subunit 5 / Pre-mRNA-processing factor 17 / Splicing factor 3B subunit 1 / : / Splicing factor 3B subunit 1 / Domain of unknown function DUF382 / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Domain of unknown function (DUF382) / G10 protein signature 2. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / BUD31/G10-related, conserved site / G10 protein signature 1. / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / : / STL11, N-terminal / Zinc-finger of C2H2 type / PHF5-like / PHF5-like protein / SKI-interacting protein SKIP, SNW domain / PSP, proline-rich / SKI-interacting protein, SKIP / SKIP/SNW domain / PSP / proline-rich domain in spliceosome associated proteins / Pre-mRNA-splicing factor Cwf15/Cwc15 / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Cwf15/Cwc15 cell cycle control protein / Splicing factor 3B subunit 10 (SF3b10) / : / WD repeat Prp46/PLRG1-like / Splicing factor 3B subunit 1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / Matrin/U1-C, C2H2-type zinc finger / Zinc finger matrin-type profile. / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / Myb-like DNA-binding domain / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / : / Zinc finger, CCCH-type superfamily / PPP2R1A-like HEAT repeat / SAP motif profile. / zinc finger / SAP domain / Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation / SAP domain / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Pre-mRNA-splicing factor Syf1-like / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / Myb-type HTH DNA-binding domain profile. / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Myb domain / RSE1/DDB1/CPSF1 second beta-propeller / Cleavage/polyadenylation specificity factor, A subunit, C-terminal / Cleavage/polyadenylation specificity factor, A subunit, N-terminal / : / CPSF A subunit region / RSE1/DDB1/CPSF1 first beta-propeller / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / Zinc finger, C3HC4 type (RING finger) / Elongation Factor G, domain II / Elongation Factor G, domain III / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus

Component:

E3 ubiquitin-protein ligase RNF113A / Pleiotropic regulator 1 / Pre-mRNA-processing factor 17 / Splicing factor 3B subunit 1 / Protein BUD31 homolog / Splicing factor 3B subunit 2 / SNW domain-containing protein 1 / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing factor 3B subunit 3 / Splicing factor 3A subunit 2 / Pre-mRNA-processing-splicing factor 8 / Spliceosome-associated protein CWC27 homolog / PHD finger-like domain-containing protein 5A / Serine/arginine repetitive matrix protein 1 / Cell division cycle 5-like protein / BUD13 homolog / Splicing factor 3B subunit 5 / Crooked neck-like protein 1 / Pre-mRNA-splicing factor RBM22 / Spliceosome-associated protein CWC15 homolog / Serine/arginine repetitive matrix protein 2 / RNA-binding motif protein, X-linked 2 / Splicing factor 3B subunit 6 / Peptidyl-prolyl cis-trans isomerase-like 1

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.4 Å
• Authors: Haselbach D / Komarov I / Agafonov D / Hartmuth K / Graf B / Kastner B
• Citation: Journal: Cell / Year: 2018; Title: Structure and Conformational Dynamics of the Human Spliceosomal B Complex.; Authors: David Haselbach / Ilya Komarov / Dmitry E Agafonov / Klaus Hartmuth / Benjamin Graf / Olexandr Dybkov / Henning Urlaub / Berthold Kastner / Reinhard Lührmann / Holger Stark / ; Abstract: The spliceosome is a highly dynamic macromolecular complex that precisely excises introns from pre-mRNA. Here we report the cryo-EM 3D structure of the human B spliceosome at 3.4 Å resolution. In the B state, the spliceosome is activated but not catalytically primed, so that it is functionally blocked prior to the first catalytic step of splicing. The spliceosomal core is similar to the yeast B spliceosome; important differences include the presence of the RNA helicase aquarius and peptidyl prolyl isomerases. To examine the overall dynamic behavior of the purified spliceosome, we developed a principal component analysis-based approach. Calculating the energy landscape revealed eight major conformational states, which we refined to higher resolution. Conformational differences of the highly flexible structural components between these eight states reveal how spliceosomal components contribute to the assembly of the spliceosome, allowing it to generate a dynamic interaction network required for its subsequent catalytic activation.

History

Deposition	Jan 3, 2018	-
Header (metadata) release	Aug 29, 2018	-
Map release	Aug 29, 2018	-
Update	Nov 6, 2024	-
Current status	Nov 6, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_4255.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.16 Å

Density

Contour Level	By AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum	-0.14888132 - 0.31364983
Average (Standard dev.)	0.0006721058 (±0.0072913566)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 487.19998 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.16	1.16	1.16
M x/y/z	420	420	420
origin x/y/z	0.000	0.000	0.000
length x/y/z	487.200	487.200	487.200
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	420	420	420
D min/max/mean	-0.149	0.314	0.001

Supplemental data

Sample components

Entire : human Bact spliceosome state 1 unmasked

• Entire: Name: human Bact spliceosome state 1 unmasked

Components

• Complex: human Bact spliceosome state 1 unmasked
  • Protein or peptide: RNA-binding motif protein, X-linked 2
  • RNA: U2 snRNA
  • Protein or peptide: BUD13 homolog
  • RNA: U5 snRNA
  • Protein or peptide: Splicing factor 3A subunit 2
  • Protein or peptide: Splicing factor 3B subunit 2
  • Protein or peptide: Pre-mRNA-processing-splicing factor 8
  • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
  • Protein or peptide: SNW domain-containing protein 1
  • Protein or peptide: Pleiotropic regulator 1
  • Protein or peptide: Pre-mRNA-processing factor 17
  • Protein or peptide: Cell division cycle 5-like protein
  • Protein or peptide: Crooked neck-like protein 1
  • Protein or peptide: Pre-mRNA-splicing factor RBM22
  • Protein or peptide: Protein BUD31 homolog
  • Protein or peptide: Spliceosome-associated protein CWC15 homolog
  • Protein or peptide: Serine/arginine repetitive matrix protein 2
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 1
  • Protein or peptide: Serine/arginine repetitive matrix protein 1
  • RNA: pre mRNA
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase CWC27 homolog
  • Protein or peptide: RING finger protein 113A
  • Protein or peptide: Splicing factor 3B subunit 1
  • Protein or peptide: Splicing factor 3B subunit 3
  • Protein or peptide: Splicing factor 3B subunit 5
  • Protein or peptide: PHD finger-like domain-containing protein 5A
  • Protein or peptide: Splicing factor 3B subunit 6
• RNA: U6 snRNA
• Ligand: MAGNESIUM ION
• Ligand: INOSITOL HEXAKISPHOSPHATE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: human Bact spliceosome state 1 unmasked

• Supramolecule: Name: human Bact spliceosome state 1 unmasked / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4, #6-#28
• Source (natural): Organism: Homo sapiens (human) / Cell: HeLa
• Molecular weight: Theoretical: 4.5 MDa

Macromolecule #1: RNA-binding motif protein, X-linked 2

• Macromolecule: Name: RNA-binding motif protein, X-linked 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.425984 KDa

Sequence

String:

MNPLTKVKLI NELNEREVQL GVADKVSWHS EYKDSAWIFL GGLPYELTEG DIICVFSQYG EIVNINLVRD KKTGKSKGFC FLCYEDQRS TILAVDNFNG IKIKGRTIRV DHVSNYRAPK DSEEIDDVTR QLQEKGCGAR TPSPSLSESS EDEKPTKKHK K DKKEKKKK KKEKEKADRE VQAEQPSSSS PRRKTVKEKD DTGPKKHSSK NSERAQKSEP REGQKLPKSR TAYSGGAEDL ER ELKKEKP KHEHKSSSRR EAREEKTRIR DRGRSSDAHS SWYNGRSEGR SYRSRSRSRD KSHRHKRARR SRERESSNPS DRW RH

UniProtKB: RNA-binding motif protein, X-linked 2

Macromolecule #3: BUD13 homolog

• Macromolecule: Name: BUD13 homolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 70.669211 KDa

Sequence

String:

MAAAPPLSKA EYLKRYLSGA DAGVDRGSES GRKRRKKRPK PGGAGGKGMR IVDDDVSWTA ISTTKLEKEE EEDDGDLPVV AEFVDERPE EVKQMEAFRS SAKWKLLGGH NEDLPSNRHF RHDTPDSSPR RVRHGTPDPS PRKDRHDTPD PSPRRARHDT P DPSPLRGA RHDSDTSPPR RIRHDSSDTS PPRRARHDSP DPSPPRRPQH NSSGASPRRV RHDSPDPSPP RRARHGSSDI SS PRRVHNN SPDTSRRTLG SSDTQQLRRA RHDSPDLAPN VTYSLPRTKS GKAPERASSK TSPHWKESGA SHLSFPKNSK YEY DPDISP PRKKQAKSHF GDKKQLDSKG DCQKATDSDL SSPRHKQSPG HQDSDSDLSP PRNRPRHRSS DSDLSPPRRR QRTK SSDSD LSPPRRSQPP GKKAAHMYSG AKTGLVLTDI QREQQELKEQ DQETMAFEAE FQYAETVFRD KSGRKRNLKL ERLEQ RRKA EKDSERDELY AQWGKGLAQS RQQQQNVEDA MKEMQKPLAR YIDDEDLDRM LREQEREGDP MANFIKKNKA KENKNK KVR PRYSGPAPPP NRFNIWPGYR WDGVDRSNGF EQKRFARLAS KKAVEELAYK WSVEDM

UniProtKB: BUD13 homolog

Macromolecule #6: Splicing factor 3A subunit 2

• Macromolecule: Name: Splicing factor 3A subunit 2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.327355 KDa

Sequence

String:

MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKE AKEAPAQPAP EKVKVEVKKF VKIGRPGYKV TKQRDSEMGQ QSLLFQIDYP EIAEGIMPRH RFMSAYEQRI E PPDRRWQY LLMAAEPYET IAFKVPSREI DKAEGKFWTH WNRETKQFFL QFHFKMEKPP APPSLPAGPP GVKRPPPPLM NG LPPRPPL PESLPPPPPG GLPLPPMPPT GPAPSGPPGP PQLPPPAPGV HPPAPVVHPP ASGVHPPAPG VHPPAPGVHP PAP GVHPPT SGVHPPAPGV HPPAPGVHPP APGVHPPAPG VHPPAPGVHP PPSAGVHPQA PGVHPAAPAV HPQAPGVHPP APGM HPQAP GVHPQPPGVH PSAPGVHPQP PGVHPSNPGV HPPTPMPPML RPPLPSEGPG NIPPPPPTN

UniProtKB: Splicing factor 3A subunit 2

Macromolecule #7: Splicing factor 3B subunit 2

• Macromolecule: Name: Splicing factor 3B subunit 2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.377812 KDa

Sequence

String:

MATEHPEPPK AELQLPPPPP PGHYGAWAAQ ELQAKLAEIG APIQGNREEL VERLQSYTRQ TGIVLNRPVL RGEDGDKAAP PPMSAQLPG IPMPPPPLGL PPLQPPPPPP PPPPGLGLGF PMAHPPNLGP PPPLRVGEPV ALSEEERLKL AQQQAALLMQ Q EERAKQQG DHSLKEHELL EQQKRAAVLL EQERQQEIAK MGTPVPRPPQ DMGQIGVRTP LGPRVAAPVG PVGPTPTVLP MG APVPRPR GPPPPPGDEN REMDDPSVGP KIPQALEKIL QLKESRQEEM NSQQEEEEME TDARSSLGQS ASETEEDTVS VSK KEKNRK RRNRKKKKKP QRVRGVSSES SGDREKDSTR SRGSDSPAAD VEIEYVTEEP EIYEPNFIFF KRIFEAFKLT DDVK KEKEK EPEKLDKLEN SAAPKKKGFE EEHKDSDDDS SDDEQEKKPE APKLSKKKLR RMNRFTVAEL KQLVARPDVV EMHDV TAQD PKLLVHLKAT RNSVPVPRHW CFKRKYLQGK RGIEKPPFEL PDFIKRTGIQ EMREALQEKE EQKTMKSKMR EKVRPK MGK IDIDYQKLHD AFFKWQTKPK LTIHGDLYYE GKEFETRLKE KKPGDLSDEL RISLGMPVGP NAHKVPPPWL IAMQRYG PP PSYPNLKIPG LNSPIPESCS FGYHAGGWGK PPVDETGKPL YGDVFGTNAA EFQTKTEEEE IDRTPWGELE PSDEESSE E EEEEESDEDK PDETGFITPA DSGLITPGGF SSVPAGMETP ELIELRKKKI EEAMDGSETP QLFTVLPEKR TATVGGAMM GSTHIYDMST VMSRKGPAPE LQGVEVALAP EELELDPMAM TQKYEEHVRE QQAQVEKEDF SDMVAEHAAK QKQKKRKAQP QDSRGGSKK YKEFKF

UniProtKB: Splicing factor 3B subunit 2

Macromolecule #8: Pre-mRNA-processing-splicing factor 8

• Macromolecule: Name: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 273.97425 KDa

Sequence

String:

MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

UniProtKB: Pre-mRNA-processing-splicing factor 8

Macromolecule #9: 116 kDa U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 109.560625 KDa

Sequence

String:

MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKFFDDPMLL E LAKQDVVL NYPM

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

Macromolecule #10: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 61.610703 KDa

Sequence

String:

MALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQ VDSEGKIKYD AIARQGQSKD KVIYSKYTDL VPKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV A AAMPVRAA DKLAPAQYIR YTPSQQGVAF NSGAKQRVIR MVEMQKDPME PPRFKINKKI PRGPPSPPAP VMHSPSRKMT VK EQQEWKI PPCISNWKNA KGYTIPLDKR LAADGRGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKMAQKEK EKH EEKLRE MAQKARERRA GIKTHVEKED GEARERDEIR HDRRKERQHD RNLSRAAPDK RSKLQRNENR DISEVIALGV PNPR TSNEV QYDQRLFNQS KGMDSGFAGG EDEIYNVYDQ AWRGGKDMAQ SIYRPSKNLD KDMYGDDLEA RIKTNRFVPD KEFSG SDRR QRGREGPVQF EEDPFGLDKF LEEAKQHGGS KRPSDSSRPK EHEHEGKKRR KE

UniProtKB: SNW domain-containing protein 1

Macromolecule #11: Pleiotropic regulator 1

• Macromolecule: Name: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 57.280758 KDa

Sequence

String:

MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQE VEYFVAGTHP YPPGPGVALT ADTKIQRMPS ESAAQSLAVA LPLQTKADAN RTAPSGSEYR HPGASDRPQP T AMNSIVME TGNTKNSALM AKKAPTMPKP QWHPPWKLYR VISGHLGWVR CIAVEPGNQW FVTGSADRTI KIWDLASGKL KL SLTGHIS TVRGVIVSTR SPYLFSCGED KQVKCWDLEY NKVIRHYHGH LSAVYGLDLH PTIDVLVTCS RDSTARIWDV RTK ASVHTL SGHTNAVATV RCQAAEPQII TGSHDTTIRL WDLVAGKTRV TLTNHKKSVR AVVLHPRHYT FASGSPDNIK QWKF PDGSF IQNLSGHNAI INTLTVNSDG VLVSGADNGT MHLWDWRTGY NFQRVHAAVQ PGSLDSESGI FACAFDQSES RLLTA EADK TIKVYREDDT ATEETHPVSW KPEIIKRKRF

UniProtKB: Pleiotropic regulator 1

Macromolecule #12: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.61218 KDa

Sequence

String:

MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMF APEFGPENPF RTQQMAAPRN MLSGYAEPAH INDFMFEQQR RTFATYGYAL DPSLDNHQVS AKYIGSVEEA E KNQGLTVF ETGQKKTEKR KKFKENDASN IDGFLGPWAK YVDEKDVAKP SEEEQKELDE ITAKRQKKGK QEEEKPGEEK TI LHVKEMY DYQGRSYLHI PQDVGVNLRS TMPPEKCYLP KKQIHVWSGH TKGVSAVRLF PLSGHLLLSC SMDCKIKLWE VYG ERRCLR TFIGHSKAVR DICFNTAGTQ FLSAAYDRYL KLWDTETGQC ISRFTNRKVP YCVKFNPDED KQNLFVAGMS DKKI VQWDI RSGEIVQEYD RHLGAVNTIV FVDENRRFVS TSDDKSLRVW EWDIPVDFKY IAEPSMHSMP AVTLSPNGKW LACQS MDNQ ILIFGAQNRF RLNKKKIFKG HMVAGYACQV DFSPDMSYVI SGDGNGKLNI WDWKTTKLYS RFKAHDKVCI GAVWHP HET SKVITCGWDG LIKLWD

UniProtKB: Pre-mRNA-processing factor 17

Macromolecule #13: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 92.406883 KDa

Sequence

String:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

Macromolecule #14: Crooked neck-like protein 1

• Macromolecule: Name: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.610008 KDa

Sequence

String:

MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA GKQRIPKVAK VKNKAPAEVQ ITAEQLLREA KERELELLPP PPQQKITDEE ELNDYKLRKR KTFEDNIRKN RT VISNWIK YAQWEESLKE IQRARSIYER ALDVDYRNIT LWLKYAEMEM KNRQVNHARN IWDRAITTLP RVNQFWYKYT YME EMLGNV AGARQVFERW MEWQPEEQAW HSYINFELRY KEVDRARTIY ERFVLVHPDV KNWIKYARFE EKHAYFAHAR KVYE RAVEF FGDEHMDEHL YVAFAKFEEN QKEFERVRVI YKYALDRISK QDAQELFKNY TIFEKKFGDR RGIEDIIVSK RRFQY EEEV KANPHNYDAW FDYLRLVESD AEAEAVREVY ERAIANVPPI QEKRHWKRYI YLWINYALYE ELEAKDPERT RQVYQA SLE LIPHKKFTFA KMWILYAQFE IRQKNLSLAR RALGTSIGKC PKNKLFKVYI ELELQLREFD RCRKLYEKFL EFGPENC TS WIKFAELETI LGDIDRARAI YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW ISFAQFEL S SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES

UniProtKB: Crooked neck-like protein 1

Macromolecule #15: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.959555 KDa

Sequence

String:

MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLE YGLPIQVRDA GLSFKDDMPK SDVNKEYYTQ NMEREISNSD GTRPVGMLGK ATSTSDMLLK LARTTPYYKR N RPHICSFW VKGECKRGEE CPYRHEKPTD PDDPLADQNI KDRYYGINDP VADKLLKRAS TMPRLDPPED KTITTLYVGG LG DTITETD LRNHFYQFGE IRTITVVQRQ QCAFIQFATR QAAEVAAEKS FNKLIVNGRR LNVKWGRSQA ARGKEKEKDG TTD SGIKLE PVPGLPGALP PPPAAEEEAS ANYFNLPPSG PPAVVNIALP PPPGIAPPPP PGFGPHMFHP MGPPPPFMRA PGPI HYPSQ DPQRMGAHAG KHSSP

UniProtKB: Pre-mRNA-splicing factor RBM22

Macromolecule #16: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.03285 KDa

Sequence

String:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

Macromolecule #17: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.674447 KDa

Sequence

String:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

Macromolecule #18: Serine/arginine repetitive matrix protein 2

• Macromolecule: Name: Serine/arginine repetitive matrix protein 2 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 300.255312 KDa

Sequence

String:

MYNGIGLPTP RGSGTNGYVQ RNLSLVRGRR GERPDYKGEE ELRRLEAALV KRPNPDILDH ERKRRVELRC LELEEMMEEQ GYEEQQIQE KVATFRLMLL EKDVNPGGKE ETPGQRPAVT ETHQLAELNE KKNERLRAAF GISDSYVDGS SFDPQRRARE A KQPAPEPP KPYSLVRESS SSRSPTPKQK KKKKKKDRGR RSESSSPRRE RKKSSKKKKH RSESESKKRK HRSPTPKSKR KS KDKKRKR SRSTTPAPKS RRAHRSTSAD SASSSDTSRS RSRSAAAKTH TTALAGRSPS PASGRRGEGD APFSEPGTTS TQR PSSPET ATKQPSSPYE DKDKDKKEKS ATRPSPSPER SSTGPEPPAP TPLLAERHGG SPQPLATTPL SQEPVNPPSE ASPT RDRSP PKSPEKLPQS SSSESSPPSP QPTKVSRHAS SSPESPKPAP APGSHREISS SPTSKNRSHG RAKRDKSHSH TPSRR MGRS RSPATAKRGR SRSRTPTKRG HSRSRSPQWR RSRSAQRWGR SRSPQRRGRS RSPQRPGWSR SRNTQRRGRS RSARRG RSH SRSPATRGRS RSRTPARRGR SRSRTPARRR SRSRTPTRRR SRSRTPARRG RSRSRTPARR RSRTRSPVRR RSRSRSP AR RSGRSRSRTP ARRGRSRSRT PARRGRSRSR TPARRSGRSR SRTPARRGRS RSRTPRRGRS RSRSLVRRGR SHSRTPQR R GRSGSSSERK NKSRTSQRRS RSNSSPEMKK SRISSRRSRS LSSPRSKAKS RLSLRRSLSG SSPCPKQKSQ TPPRRSRSG SSQPKAKSRT PPRRSRSSSS PPPKQKSKTP SRQSHSSSSP HPKVKSGTPP RQGSITSPQA NEQSVTPQRR SCFESSPDPE LKSRTPSRH SCSGSSPPRV KSSTPPRQSP SRSSSPQPKV KAIISPRQRS HSGSSSPSPS RVTSRTTPRR SRSVSPCSNV E SRLLPRYS HSGSSSPDTK VKPETPPRQS HSGSISPYPK VKAQTPPGPS LSGSKSPCPQ EKSKDSLVQS CPGSLSLCAG VK SSTPPGE SYFGVSSLQL KGQSQTSPDH RSDTSSPEVR QSHSESPSLQ SKSQTSPKGG RSRSSSPVTE LASRSPIRQD RGE FSASPM LKSGMSPEQS RFQSDSSSYP TVDSNSLLGQ SRLETAESKE KMALPPQEDA TASPPRQKDK FSPFPVQDRP ESSL VFKDT LRTPPRERSG AGSSPETKEQ NSALPTSSQD EELMEVVEKS EEPAGQILSH LSSELKEMST SNFESSPEVE ERPAV SLTL DQSQSQASLE AVEVPSMASS WGGPHFSPEH KELSNSPLRE NSFGSPLEFR NSGPLGTEMN TGFSSEVKED LNGPFL NQL ETDPSLDMKE QSTRSSGHSS SELSPDAVEK AGMSSNQSIS SPVLDAVPRT PSRERSSSAS SPEMKDGLPR TPSRRSR SG SSPGLRDGSG TPSRHSLSGS SPGMKDIPRT PSRGRSECDS SPEPKALPQT PRPRSRSPSS PELNNKCLTP QRERSGSE S SVDQKTVART PLGQRSRSGS SQELDVKPSA SPQERSESDS SPDSKAKTRT PLRQRSRSGS SPEVDSKSRL SPRRSRSGS SPEVKDKPRA APRAQSGSDS SPEPKAPAPR ALPRRSRSGS SSKGRGPSPE GSSSTESSPE HPPKSRTARR GSRSSPEPKT KSRTPPRRR SSRSSPELTR KARLSRRSRS ASSSPETRSR TPPRHRRSPS VSSPEPAEKS RSSRRRRSAS SPRTKTTSRR G RSPSPKPR GLQRSRSRSR REKTRTTRRR DRSGSSQSTS RRRQRSRSRS RVTRRRRGGS GYHSRSPARQ ESSRTSSRRR RG RSRTPPT SRKRSRSRTS PAPWKRSRSR ASPATHRRSR SRTPLISRRR SRSRTSPVSR RRSRSRTSVT RRRSRSRASP VSR RRSRSR TPPVTRRRSR SRTPTTRRRS RSRTPPVTRR RSRSRTPPVT RRRSRSRTSP ITRRRSRSRT SPVTRRRSRS RTSP VTRRR SRSRTSPVTR RRSRSRTPPA IRRRSRSRTP LLPRKRSRSR SPLAIRRRSR SRTPRTARGK RSLTRSPPAI RRRSA SGSS SDRSRSATPP ATRNHSGSRT PPVALNSSRM SCFSRPSMSP TPLDRCRSPG MLEPLGSSRT PMSVLQQAGG SMMDGP GPR IPDHQRTSVP ENHAQSRIAL ALTAISLGTA RPPPSMSAAG LAARMSQVPA PVPLMSLRTA PAANLASRIP AASAAAM NL ASARTPAIPT AVNLADSRTP AAAAAMNLAS PRTAVAPSAV NLADPRTPTA PAVNLAGART PAALAALSLT GSGTPPTA A NYPSSSRTPQ APASANLVGP RSAHATAPVN IAGSRTAAAL APASLTSARM APALSGANLT SPRVPLSAYE RVSGRTSPP LLDRARSRTP PSAPSQSRMT SERAPSPSSR MGQAPSQSLL PPAQDQPRSP VPSAFSDQSR CLIAQTTPVA GSQSLSSGAV ATTTSSAGD HNGMLSVPAP GVPHSDVGEP PASTGAQQPS ALAALQPAKE RRSSSSSSSS SSSSSSSSSS SSSSSSSGSS S SDSEGSSL PVQPEVALKR VPSPTPAPKE AVREGRPPEP TPAKRKRRSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SS SSSSSSS SPSPAKPGPQ ALPKPASPKK PPPGERRSRS PRKPIDSLRD SRSLSYSPVE RRRPSPQPSP RDQQSSSSER GSR RGQRGD SRSPSHKRRR ETPSPRPMRH RSSRSP

UniProtKB: Serine/arginine repetitive matrix protein 2

Macromolecule #19: Peptidyl-prolyl cis-trans isomerase-like 1

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase-like 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.257805 KDa

Sequence

String:

MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPD LKFTGAGILA MANAGPDTNG SQFFVTLAPT QWLDGKHTIF GRVCQGIGMV NRVGMVETNS QDRPVDDVKI I KAYPSG

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 1

Macromolecule #20: Serine/arginine repetitive matrix protein 1

• Macromolecule: Name: Serine/arginine repetitive matrix protein 1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 102.600539 KDa

Sequence

String:

MDAGFFRGTS AEQDNRFSNK QKKLLKQLKF AECLEKKVDM SKVNLEVIKP WITKRVTEIL GFEDDVVIEF IFNQLEVKNP DSKMMQINL TGFLNGKNAR EFMGELWPLL LSAQENIAGI PSAFLELKKE EIKQRQIEQE KLASMKKQDE DKDKRDKEEK E SSREKRER SRSPRRRKSR SPSPRRRSSP VRRERKRSHS RSPRHRTKSR SPSPAPEKKE KTPELPEPSV KVKEPSVQEA TS TSDILKV PKPEPIPEPK EPSPEKNSKK EKEKEKTRPR SRSRSKSRSR TRSRSPSHTR PRRRHRSRSR SYSPRRRPSP RRR PSPRRR TPPRRMPPPP RHRRSRSPVR RRRRSSASLS GSSSSSSSSR SRSPPKKPPK RTSSPPRKTR RLSPSASPPR RRHR PSPPA TPPPKTRHSP TPQQSNRTRK SRVSVSPGRT SGKVTKHKGT EKRESPSPAP KPRKVELSES EEDKGGKMAA ADSVQ QRRQ YRRQNQQSSS DSGSSSSSED ERPKRSHVKN GEVGRRRRHS PSRSASPSPR KRQKETSPRG RRRRSPSPPP TRRRRS PSP APPPRRRRTP TPPPRRRTPS PPPRRRSPSP RRYSPPIQRR YSPSPPPKRR TASPPPPPKR RASPSPPPKR RVSHSPP PK QRSSPVTKRR SPSLSSKHRK GSSPSRSTRE ARSPQPNKRH SPSPRPRAPQ TSSSPPPVRR GASSSPQRRQ SPSPSTRP I RRVSRTPEPK KIKKAASPSP QSVRRVSSSR SVSGSPEPAA KKPPAPPSPV QSQSPSTNWS PAVPVKKAKS PTPSPSPPR NSDQEGGGKK KKKKKDKKHK KDKKHKKHKK HKKEKAVAAA AAAAVTPAAI AAATTTLAQE EPVAAPEPKK ETESEAEDNL DDLEKHLRE KALRSMRKAQ VSPQS

UniProtKB: Serine/arginine repetitive matrix protein 1

Macromolecule #22: Peptidyl-prolyl cis-trans isomerase CWC27 homolog

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase CWC27 homolog / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 53.941227 KDa

Sequence

String:

MSNIYIQEPP TNGKVLLKTT AGDIDIELWS KEAPKACRNF IQLCLEAYYD NTIFHRVVPG FIVQGGDPTG TGSGGESIYG APFKDEFHS RLRFNRRGLV AMANAGSHDN GSQFFFTLGR ADELNNKHTI FGKVTGDTVY NMLRLSEVDI DDDERPHNPH K IKSCEVLF NPFDDIIPRE IKRLKKEKPE EEVKKLKPKG TKNFSLLSFG EEAEEEEEEV NRVSQSMKGK SKSSHDLLKD DP HLSSVPV VESEKGDAPD LVDDGEDESA EHDEYIDGDE KNLMRERIAK KLKKDTSANV KSAGEGEVEK KSVSRSEELR KEA RQLKRE LLAAKQKKVE NAAKQAEKRS EEEEAPPDGA VAEYRREKQK YEALRKQQSK KGTSREDQTL ALLNQFKSKL TQAI AETPE NDIPETEVED DEGWMSHVLQ FEDKSRKVKD ASMQDSDTFE IYDPRNPVNK RRREESKKLM REKKERR

UniProtKB: Spliceosome-associated protein CWC27 homolog

Macromolecule #23: RING finger protein 113A

• Macromolecule: Name: RING finger protein 113A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 38.847199 KDa

Sequence

String:

MAEQLSPGKA VDQVCTFLFK KPGRKGAAGR RKRPACDPEP GESGSSSDEG CTVVRPEKKR VTHNPMIQKT RDSGKQKAAY GDLSSEEEE ENEPESLGVV YKSTRSAKPV GPEDMGATAV YELDTEKERD AQAIFERSQK IQEELRGKED DKIYRGINNY Q KYMKPKDT SMGNASSGMV RKGPIRAPEH LRATVRWDYQ PDICKDYKET GFCGFGDSCK FLHDRSDYKH GWQIERELDE GR YGVYEDE NYEVGSDDEE IPFKCFICRQ SFQNPVVTKC RHYFCESCAL QHFRTTPRCY VCDQQTNGVF NPAKELIAKL EKH RATGEG GASDLPEDPD EDAIPIT

UniProtKB: E3 ubiquitin-protein ligase RNF113A

Macromolecule #24: Splicing factor 3B subunit 1

• Macromolecule: Name: Splicing factor 3B subunit 1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 146.024938 KDa

Sequence

String:

MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR FAGYVTSIAA TELEDDDDDY SSSTSLLGQK KPGYHAPVA LLNDIPQSTE QYDPFAEHRP PKIADREDEY KKHRRTMIIS PERLDPFADG GKTPDPKMNA RTYMDVMREQ H LTKEEREI RQQLAEKAKA GELKVVNGAA ASQPPSKRKR RWDQTADQTP GATPKKLSSW DQAETPGHTP SLRWDETPGR AK GSETPGA TPGSKIWDPT PSHTPAGAAT PGRGDTPGHA TPGHGGATSS ARKNRWDETP KTERDTPGHG SGWAETPRTD RGG DSIGET PTPGASKRKS RWDETPASQM GGSTPVLTPG KTPIGTPAMN MATPTPGHIM SMTPEQLQAW RWEREIDERN RPLS DEELD AMFPEGYKVL PPPAGYVPIR TPARKLTATP TPLGGMTGFH MQTEDRTMKS VNDQPSGNLP FLKPDDIQYF DKLLV DVDE STLSPEEQKE RKIMKLLLKI KNGTPPMRKA ALRQITDKAR EFGAGPLFNQ ILPLLMSPTL EDQERHLLVK VIDRIL YKL DDLVRPYVHK ILVVIEPLLI DEDYYARVEG REIISNLAKA AGLATMISTM RPDIDNMDEY VRNTTARAFA VVASALG IP SLLPFLKAVC KSKKSWQARH TGIKIVQQIA ILMGCAILPH LRSLVEIIEH GLVDEQQKVR TISALAIAAL AEAATPYG I ESFDSVLKPL WKGIRQHRGK GLAAFLKAIG YLIPLMDAEY ANYYTREVML ILIREFQSPD EEMKKIVLKV VKQCCGTDG VEANYIKTEI LPPFFKHFWQ HRMALDRRNY RQLVDTTVEL ANKVGAAEII SRIVDDLKDE AEQYRKMVME TIEKIMGNLG AADIDHKLE EQLIDGILYA FQEQTTEDSV MLNGFGTVVN ALGKRVKPYL PQICGTVLWR LNNKSAKVRQ QAADLISRTA V VMKTCQEE KLMGHLGVVL YEYLGEEYPE VLGSILGALK AIVNVIGMHK MTPPIKDLLP RLTPILKNRH EKVQENCIDL VG RIADRGA EYVSAREWMR ICFELLELLK AHKKAIRRAT VNTFGYIAKA IGPHDVLATL LNNLKVQERQ NRVCTTVAIA IVA ETCSPF TVLPALMNEY RVPELNVQNG VLKSLSFLFE YIGEMGKDYI YAVTPLLEDA LMDRDLVHRQ TASAVVQHMS LGVY GFGCE DSLNHLLNYV WPNVFETSPH VIQAVMGALE GLRVAIGPCR MLQYCLQGLF HPARKVRDVY WKIYNSIYIG SQDAL IAHY PRIYNDDKNT YIRYELDYIL

UniProtKB: Splicing factor 3B subunit 1

Macromolecule #25: Splicing factor 3B subunit 3

• Macromolecule: Name: Splicing factor 3B subunit 3 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 135.718844 KDa

Sequence

String:

MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY HVVGVDVGFE NPMFACLEMD YEEADNDPTG EAAANTQQTL TFYELDLGLN HVVRKYSEPL EEHGNFLITV PG GSDGPSG VLICSENYIT YKNFGDQPDI RCPIPRRRND LDDPERGMIF VCSATHKTKS MFFFLAQTEQ GDIFKITLET DED MVTEIR LKYFDTVPVA AAMCVLKTGF LFVASEFGNH YLYQIAHLGD DDEEPEFSSA MPLEEGDTFF FQPRPLKNLV LVDE LDSLS PILFCQIADL ANEDTPQLYV ACGRGPRSSL RVLRHGLEVS EMAVSELPGN PNAVWTVRRH IEDEFDAYII VSFVN ATLV LSIGETVEEV TDSGFLGTTP TLSCSLLGDD ALVQVYPDGI RHIRADKRVN EWKTPGKKTI VKCAVNQRQV VIALTG GEL VYFEMDPSGQ LNEYTERKEM SADVVCMSLA NVPPGEQRSR FLAVGLVDNT VRIISLDPSD CLQPLSMQAL PAQPESL CI VEMGGTEKQD ELGERGSIGF LYLNIGLQNG VLLRTVLDPV TGDLSDTRTR YLGSRPVKLF RVRMQGQEAV LAMSSRSW L SYSYQSRFHL TPLSYETLEF ASGFASEQCP EGIVAISTNT LRILALEKLG AVFNQVAFPL QYTPRKFVIH PESNNLIII ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA AFLNENLPES IFGAPKAGNG QWASVIRVMN PIQGNTLDLV QLEQNEAAF SVAVCRFSNT GEDWYVLVGV AKDLILNPRS VAGGFVYTYK LVNNGEKLEF LHKTPVEEVP AAIAPFQGRV L IGVGKLLR VYDLGKKKLL RKCENKHIAN YISGIQTIGH RVIVSDVQES FIWVRYKRNE NQLIIFADDT YPRWVTTASL LD YDTVAGA DKFGNICVVR LPPNTNDEVD EDPTGNKALW DRGLLNGASQ KAEVIMNYHV GETVLSLQKT TLIPGGSESL VYT TLSGGI GILVPFTSHE DHDFFQHVEM HLRSEHPPLC GRDHLSFRSY YFPVKNVIDG DLCEQFNSME PNKQKNVSEE LDRT PPEVS KKLEDIRTRY AF

UniProtKB: Splicing factor 3B subunit 3

Macromolecule #26: Splicing factor 3B subunit 5

• Macromolecule: Name: Splicing factor 3B subunit 5 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.149369 KDa

Sequence

String:

MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL NYFAIAENES KARVRFNLME KMLQPCGPPA DKPEEN

UniProtKB: Splicing factor 3B subunit 5

Macromolecule #27: PHD finger-like domain-containing protein 5A

• Macromolecule: Name: PHD finger-like domain-containing protein 5A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.427524 KDa

Sequence

String:

MAKHHPDLIF CRKQAGVAIG RLCEKCDGKC VICDSYVRPC TLVRICDECN YGSYQGRCVI CGGPGVSDAY YCKECTIQEK DRDGCPKIV NLGSSKTDLF YERKKYGFKK R

UniProtKB: PHD finger-like domain-containing protein 5A

Macromolecule #28: Splicing factor 3B subunit 6

• Macromolecule: Name: Splicing factor 3B subunit 6 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.6069 KDa

Sequence

String:

MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVV LYYNANRAFQ KMDTKKKEEQ LKLLKEKYGI NTDPPK

UniProtKB: Splicing factor 3B subunit 6

Macromolecule #2: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 60.186445 KDa

Sequence

String:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUGGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

GENBANK: GENBANK: X02665.1

Macromolecule #4: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.908668 KDa

Sequence

String:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

GENBANK: GENBANK: X01691.1

Macromolecule #5: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.404438 KDa

Sequence

String:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUUU

Macromolecule #21: pre mRNA

• Macromolecule: Name: pre mRNA / type: rna / ID: 21 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 153.787984 KDa

Sequence

String:

GAAUACAAGC UCAUCCGAUA UCCGUACACC AUCAGGGUAC GAGCUAGCCC AUGGCGUACA CCAUCAGGGU ACGACUAGUA GAUCUCGUA CACCAUCAGG GUACGGAAUU CUCUAGAGUC GAGGAGGACA UCUCAGCAAA AGAGAAGCUG CUGCGGGCGU C GGAGGACG AGCGGGACCG GGUGCUGGAG GAGCUGCACA AGGCAGAGGA CAGCCUGCUG GCUGCCGACG AGACCGCCGC CA AGGUAUG UAUCAAGCUU ACAAGACAGC UUUAAGGAGA CCAAUAGAAA CUGGGCAUGU GGAGACAGAG AAGACUCUUG GCC UCGAGA AACCUGUAAC UGGAAUGUGU GUGGAGUGUG ACUGAUAGAA CACUACCUGA UUCUUAUGUA UUUACUGACC UGUG UUUUU UUGCUACUUU UUUUCUUUUC UCCCCUUCCC CUUUCCCUAU UUUUUUUCUU GCCCUGAUCC GGAAUUUGGA UCC

Macromolecule #29: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 29 / Number of copies: 4 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #30: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 30 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Macromolecule #31: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 31 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #32: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 32 / Number of copies: 10 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.05 mg/mL

Buffer

pH: 7.9
Component:

Concentration	Name	Formula
20.0 mM	HEPES
150.0 mM		NaCl
1.5 mM		MgCl2

• Grid: Model: Quantifoil R3.5/1 / Material: COPPER / Mesh: 400 / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: HOLEY / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: CARBON / Support film - #1 - topology: CONTINUOUS
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 75 % / Chamber temperature: 277 K / Instrument: LEICA EM GP / Details: blot with blotting sensor.

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Alignment procedure: Coma free - Residual tilt: 14.0 mrad
• Specialist optics: Spherical aberration corrector: Microscope was modified with a Cs corrector with two hexapoles elements
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 32000 / Average exposure time: 1.0 sec. / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 70.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 0.001 mm / Nominal defocus max: 4.5 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 59000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 308000
• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 17000
• Initial angle assignment: Type: OTHER / Software - Name: EMAN2
• Final angle assignment: Type: PROJECTION MATCHING / Software - Name: RELION
• Final 3D classification: Number classes: 220 / Software - Name: RELION

Atomic model buiding 1

• Refinement: Protocol: RIGID BODY FIT

Output model

PDB-6ff4:
human Bact spliceosome core structure