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基本情報
登録情報 | ![]() | |||||||||
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タイトル | In-situ structure of typeX supercomplex in respiratory chain (composite) | |||||||||
![]() | In-situ structure of supercomplex in respiratory chain ( typeX ) | |||||||||
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![]() | in-situ cryo-EM structure / mammalian / mitochondria / respiratory supercomplex / proton pumping / membrane protein / electron transport | |||||||||
機能・相同性 | ![]() Complex IV assembly / Cytoprotection by HMOX1 / TP53 Regulates Metabolic Genes / Complex III assembly / subthalamus development / pons development / cerebellar Purkinje cell layer development / respiratory chain complex IV assembly / mitochondrial respirasome assembly / RHOG GTPase cycle ...Complex IV assembly / Cytoprotection by HMOX1 / TP53 Regulates Metabolic Genes / Complex III assembly / subthalamus development / pons development / cerebellar Purkinje cell layer development / respiratory chain complex IV assembly / mitochondrial respirasome assembly / RHOG GTPase cycle / pyramidal neuron development / Complex I biogenesis / thalamus development / Respiratory electron transport / Neutrophil degranulation / Mitochondrial protein degradation / respiratory chain complex IV / respiratory chain complex / cytochrome-c oxidase / respiratory chain complex III / oxidative phosphorylation / cardiac muscle tissue development / quinol-cytochrome-c reductase / oxidoreductase activity, acting on NAD(P)H / mitochondrial electron transport, cytochrome c to oxygen / quinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / mitochondrial electron transport, ubiquinol to cytochrome c / hypothalamus development / midbrain development / ubiquinone binding / electron transport coupled proton transport / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase activity / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / response to cAMP / enzyme regulator activity / positive regulation of vasoconstriction / reactive oxygen species metabolic process / aerobic respiration / hippocampus development / central nervous system development / respiratory electron transport chain / electron transport chain / brain development / metalloendopeptidase activity / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / NAD binding / fatty acid biosynthetic process / FMN binding / nervous system development / 4 iron, 4 sulfur cluster binding / electron transfer activity / mitochondrial inner membrane / oxidoreductase activity / nuclear speck / nuclear body / mitochondrial matrix / copper ion binding / heme binding / mitochondrion / proteolysis / nucleoplasm / metal ion binding / membrane 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 6.5 Å | |||||||||
![]() | Zheng W / Zhang K / Zhu J | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: High-resolution in situ structures of mammalian respiratory supercomplexes. 著者: Wan Zheng / Pengxin Chai / Jiapeng Zhu / Kai Zhang / ![]() ![]() 要旨: Mitochondria play a pivotal part in ATP energy production through oxidative phosphorylation, which occurs within the inner membrane through a series of respiratory complexes. Despite extensive in ...Mitochondria play a pivotal part in ATP energy production through oxidative phosphorylation, which occurs within the inner membrane through a series of respiratory complexes. Despite extensive in vitro structural studies, determining the atomic details of their molecular mechanisms in physiological states remains a major challenge, primarily because of loss of the native environment during purification. Here we directly image porcine mitochondria using an in situ cryo-electron microscopy approach. This enables us to determine the structures of various high-order assemblies of respiratory supercomplexes in their native states. We identify four main supercomplex organizations: IIIIIV, IIIIIV, IIIIIV and IIIIIV, which potentially expand into higher-order arrays on the inner membranes. These diverse supercomplexes are largely formed by 'protein-lipids-protein' interactions, which in turn have a substantial impact on the local geometry of the surrounding membranes. Our in situ structures also capture numerous reactive intermediates within these respiratory supercomplexes, shedding light on the dynamic processes of the ubiquinone/ubiquinol exchange mechanism in complex I and the Q-cycle in complex III. Structural comparison of supercomplexes from mitochondria treated under different conditions indicates a possible correlation between conformational states of complexes I and III, probably in response to environmental changes. By preserving the native membrane environment, our approach enables structural studies of mitochondrial respiratory supercomplexes in reaction at high resolution across multiple scales, from atomic-level details to the broader subcellular context. | |||||||||
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マップデータ | ![]() | 15.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 102.5 KB 102.5 KB | 表示 表示 | ![]() |
画像 | ![]() | 113.1 KB | ||
Filedesc metadata | ![]() | 20.4 KB | ||
その他 | ![]() ![]() | 474.7 MB 474.7 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8ugrMC ![]() 8ud1C ![]() 8ueoC ![]() 8uepC ![]() 8ueqC ![]() 8uerC ![]() 8uesC ![]() 8uetC ![]() 8ueuC ![]() 8uevC ![]() 8uewC ![]() 8uexC ![]() 8ueyC ![]() 8uezC ![]() 8ugdC ![]() 8ugeC ![]() 8ugfC ![]() 8uggC ![]() 8ughC ![]() 8ugiC ![]() 8ugjC ![]() 8ugkC ![]() 8uglC ![]() 8ugnC ![]() 8ugpC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | In-situ structure of supercomplex in respiratory chain ( typeX ) | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.56 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: In-situ structure of supercomplex in respiratory chain ( typeX )
ファイル | emd_42233_half_map_1.map | ||||||||||||
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注釈 | In-situ structure of supercomplex in respiratory chain ( typeX ) | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: In-situ structure of supercomplex in respiratory chain ( typeX )
ファイル | emd_42233_half_map_2.map | ||||||||||||
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注釈 | In-situ structure of supercomplex in respiratory chain ( typeX ) | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : In-situ cryo-EM structure of respiratory supercomplex by directly...
+超分子 #1: In-situ cryo-EM structure of respiratory supercomplex by directly...
+分子 #1: NADH-ubiquinone oxidoreductase chain 3
+分子 #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
+分子 #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
+分子 #4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
+分子 #5: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
+分子 #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
+分子 #7: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
+分子 #8: NADH-ubiquinone oxidoreductase chain 1
+分子 #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
+分子 #10: NADH-ubiquinone oxidoreductase chain 6
+分子 #11: NADH-ubiquinone oxidoreductase chain 4L
+分子 #12: NADH-ubiquinone oxidoreductase chain 5
+分子 #13: NADH-ubiquinone oxidoreductase chain 4
+分子 #14: NADH-ubiquinone oxidoreductase chain 2
+分子 #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...
+分子 #16: NADH:ubiquinone oxidoreductase subunit A9
+分子 #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
+分子 #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
+分子 #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
+分子 #20: NADH:ubiquinone oxidoreductase subunit AB1
+分子 #21: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isof...
+分子 #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
+分子 #23: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
+分子 #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
+分子 #25: NADH:ubiquinone oxidoreductase subunit A13
+分子 #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
+分子 #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
+分子 #28: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
+分子 #29: NADH dehydrogenase [ubiquinone] 1 subunit C2
+分子 #30: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
+分子 #31: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
+分子 #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...
+分子 #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...
+分子 #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
+分子 #35: NADH:ubiquinone oxidoreductase subunit B2
+分子 #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
+分子 #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...
+分子 #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
+分子 #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
+分子 #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
+分子 #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
+分子 #42: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
+分子 #43: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
+分子 #44: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
+分子 #45: Cytochrome b-c1 complex subunit 1, mitochondrial
+分子 #46: Cytochrome b-c1 complex subunit 2, mitochondrial
+分子 #47: Cytochrome b
+分子 #48: Cytochrome c1
+分子 #49: Cytochrome b-c1 complex subunit Rieske, mitochondrial
+分子 #50: Cytochrome b-c1 complex subunit 7
+分子 #51: Cytochrome b-c1 complex subunit 8
+分子 #52: Cytochrome b-c1 complex subunit 6, mitochondrial
+分子 #53: Ubiquinol-cytochrome c reductase complex 7.2 kDa protein
+分子 #54: Cytochrome b-c1 complex subunit 10
+分子 #55: Cytochrome c oxidase subunit 1
+分子 #56: Cytochrome c oxidase subunit 2
+分子 #57: Cytochrome c oxidase subunit 3
+分子 #58: Cytochrome c oxidase subunit 4
+分子 #59: Cytochrome c oxidase subunit 5A, mitochondrial
+分子 #60: Cytochrome c oxidase subunit 5B, mitochondrial
+分子 #61: Cytochrome c oxidase subunit 6A2
+分子 #62: Cytochrome c oxidase subunit 6B1
+分子 #63: Cytochrome c oxidase subunit 6C
+分子 #64: Cytochrome c oxidase subunit 7A1, mitochondrial
+分子 #65: Cytochrome c oxidase subunit 7B
+分子 #66: Cytochrome c oxidase subunit 7C, mitochondrial
+分子 #67: Cytochrome c oxidase subunit 8
+分子 #68: Cytochrome c oxidase subunit NDUFA4
+分子 #69: 1,2-Distearoyl-sn-glycerophosphoethanolamine
+分子 #70: IRON/SULFUR CLUSTER
+分子 #71: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
+分子 #72: FE2/S2 (INORGANIC) CLUSTER
+分子 #73: FLAVIN MONONUCLEOTIDE
+分子 #74: POTASSIUM ION
+分子 #75: CARDIOLIPIN
+分子 #76: GUANOSINE-5'-TRIPHOSPHATE
+分子 #77: MAGNESIUM ION
+分子 #78: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
+分子 #79: ZINC ION
+分子 #80: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butan...
+分子 #81: N-ACETYLMETHIONINE
+分子 #82: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-...
+分子 #83: PROTOPORPHYRIN IX CONTAINING FE
+分子 #84: HEME C
+分子 #85: HEME-A
+分子 #86: COPPER (II) ION
+分子 #87: SODIUM ION
+分子 #88: DINUCLEAR COPPER ION
+分子 #89: (7R,17E,20E)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)MET...
+分子 #90: (1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(STEAROYLOXY)...
+分子 #91: PHOSPHATE ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI POLARA 300 |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 3.0 µm / 最小 デフォーカス(公称値): 1.3 µm |
実験機器 | ![]() モデル: Tecnai Polara / 画像提供: FEI Company |