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Yorodumi- EMDB-41885: Structure of the HER2/HER4/BTC Heterodimer Extracellular Domain -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-41885 | ||||||||||||
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Title | Structure of the HER2/HER4/BTC Heterodimer Extracellular Domain | ||||||||||||
Map data | |||||||||||||
Sample |
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Keywords | Receptor Tyrosine Kinase / MEMBRANE PROTEIN / TRANSFERASE | ||||||||||||
Function / homology | Function and homology information establishment of planar polarity involved in nephron morphogenesis / ERBB4 signaling pathway / epidermal growth factor receptor activity / ERBB4-ERBB4 signaling pathway / olfactory bulb interneuron differentiation / central nervous system morphogenesis / Inhibition of Signaling by Overexpressed EGFR / neuregulin receptor activity / EGFR interacts with phospholipase C-gamma / cardiac muscle tissue regeneration ...establishment of planar polarity involved in nephron morphogenesis / ERBB4 signaling pathway / epidermal growth factor receptor activity / ERBB4-ERBB4 signaling pathway / olfactory bulb interneuron differentiation / central nervous system morphogenesis / Inhibition of Signaling by Overexpressed EGFR / neuregulin receptor activity / EGFR interacts with phospholipase C-gamma / cardiac muscle tissue regeneration / negative regulation of immature T cell proliferation in thymus / ERBB3:ERBB2 complex / PLCG1 events in ERBB2 signaling / ERBB2-ERBB4 signaling pathway / GRB7 events in ERBB2 signaling / immature T cell proliferation in thymus / RNA polymerase I core binding / mitochondrial fragmentation involved in apoptotic process / ERBB2-EGFR signaling pathway / ERBB2 Activates PTK6 Signaling / semaphorin receptor complex / GABA receptor binding / Signaling by EGFR / transmembrane receptor protein tyrosine kinase activator activity / positive regulation of urine volume / PI3K events in ERBB4 signaling / negative regulation of epithelial cell apoptotic process / mammary gland epithelial cell differentiation / embryonic pattern specification / Signaling by ERBB4 / regulation of microtubule-based process / positive regulation of protein localization to cell surface / ErbB-3 class receptor binding / ERBB2 Regulates Cell Motility / Sema4D induced cell migration and growth-cone collapse / motor neuron axon guidance / PI3K events in ERBB2 signaling / neural crest cell migration / epithelial cell apoptotic process / neurotransmitter receptor localization to postsynaptic specialization membrane / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / positive regulation of Rho protein signal transduction / epidermal growth factor receptor binding / neuromuscular junction development / Drug-mediated inhibition of ERBB2 signaling / Resistance of ERBB2 KD mutants to trastuzumab / Resistance of ERBB2 KD mutants to sapitinib / Resistance of ERBB2 KD mutants to tesevatinib / Resistance of ERBB2 KD mutants to neratinib / Resistance of ERBB2 KD mutants to osimertinib / Resistance of ERBB2 KD mutants to afatinib / Resistance of ERBB2 KD mutants to AEE788 / Resistance of ERBB2 KD mutants to lapatinib / Drug resistance in ERBB2 TMD/JMD mutants / cell fate commitment / GAB1 signalosome / enzyme-linked receptor protein signaling pathway / ERBB2-ERBB3 signaling pathway / positive regulation of transcription by RNA polymerase I / oligodendrocyte differentiation / cell surface receptor signaling pathway via JAK-STAT / Long-term potentiation / semaphorin-plexin signaling pathway / positive regulation of cell division / cellular response to epidermal growth factor stimulus / Signaling by ERBB2 / positive regulation of cell adhesion / SHC1 events in ERBB4 signaling / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / positive regulation of protein targeting to membrane / GABA-ergic synapse / mammary gland alveolus development / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / GRB2 events in ERBB2 signaling / transmembrane receptor protein tyrosine kinase activity / regulation of angiogenesis / neurogenesis / SHC1 events in ERBB2 signaling / coreceptor activity / regulation of ERK1 and ERK2 cascade / Schwann cell development / basal plasma membrane / Nuclear signaling by ERBB4 / positive regulation of epithelial cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / positive regulation of cardiac muscle cell proliferation / cell surface receptor protein tyrosine kinase signaling pathway / synapse assembly / Downregulation of ERBB4 signaling / myelination / lactation / regulation of cell migration / phosphatidylinositol 3-kinase/protein kinase B signal transduction / Downregulation of ERBB2:ERBB3 signaling / Constitutive Signaling by Overexpressed ERBB2 / positive regulation of mitotic nuclear division / EGFR downregulation / Signaling by ERBB2 TMD/JMD mutants / positive regulation of MAP kinase activity Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.27 Å | ||||||||||||
Authors | Trenker R / Diwanji D / Bingham T / Verba KA / Jura N | ||||||||||||
Funding support | Germany, United States, 3 items
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Citation | Journal: To Be Published Title: Structure of the HER2/HER4/BTC Heterodimer Extracellular Domain Authors: Trenker R / Diwanji D / Bingham T / Verba KA / Jura N | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_41885.map.gz | 203.6 MB | EMDB map data format | |
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Header (meta data) | emd-41885-v30.xml emd-41885.xml | 19.9 KB 19.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_41885_fsc.xml emd_41885_fsc_2.xml | 13.4 KB 17.7 KB | Display Display | FSC data file |
Images | emd_41885.png | 50 KB | ||
Masks | emd_41885_msk_1.map | 216 MB | Mask map | |
Filedesc metadata | emd-41885.cif.gz | 7 KB | ||
Others | emd_41885_half_map_1.map.gz emd_41885_half_map_2.map.gz | 200.6 MB 200.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-41885 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-41885 | HTTPS FTP |
-Validation report
Summary document | emd_41885_validation.pdf.gz | 1.2 MB | Display | EMDB validaton report |
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Full document | emd_41885_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | emd_41885_validation.xml.gz | 21.7 KB | Display | |
Data in CIF | emd_41885_validation.cif.gz | 28.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-41885 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-41885 | HTTPS FTP |
-Related structure data
Related structure data | 8u4kMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_41885.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.835 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_41885_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_41885_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_41885_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Ternary complex of HER2/HER4/BTC
Entire | Name: Ternary complex of HER2/HER4/BTC |
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Components |
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-Supramolecule #1: Ternary complex of HER2/HER4/BTC
Supramolecule | Name: Ternary complex of HER2/HER4/BTC / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 Details: HER2 and HER4 Receptors were expressed in EXPI293F cells. The ligand BTC was expressed in E. coli Origami B (DE3) |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 284.435 KDa |
-Macromolecule #1: Isoform JM-A CYT-1 of Receptor tyrosine-protein kinase erbB-4
Macromolecule | Name: Isoform JM-A CYT-1 of Receptor tyrosine-protein kinase erbB-4 type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: receptor protein-tyrosine kinase |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 67.909648 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: QSVCAGTENK LSSLSDLEQQ YRALRKYYEN CEVVMGNLEI TSIEHNRDLS FLRSVREVTG YVLVALNQFR YLPLENLRII RGTKLYEDR YALAIFLNYR KDGNFGLQEL GLKNLTEILN GGVYVDQNKF LCYADTIHWQ DIVRNPWPSN LTLVSTNGSS G CGRCHKSC ...String: QSVCAGTENK LSSLSDLEQQ YRALRKYYEN CEVVMGNLEI TSIEHNRDLS FLRSVREVTG YVLVALNQFR YLPLENLRII RGTKLYEDR YALAIFLNYR KDGNFGLQEL GLKNLTEILN GGVYVDQNKF LCYADTIHWQ DIVRNPWPSN LTLVSTNGSS G CGRCHKSC TGRCWGPTEN HCQTLTRTVC AEQCDGRCYG PYVSDCCHRE CAGGCSGPKD TDCFACMNFN DSGACVTQCP QT FVYNPTT FQLEHNFNAK YTYGAFCVKK CPHNFVVDSS SCVRACPSSK MEVEENGIKM CKPCTDICPK ACDGIGTGSL MSA QTVDSS NIDKFINCTK INGNLIFLVT GIHGDPYNAI EAIDPEKLNV FRTVREITGF LNIQSWPPNM TDFSVFSNLV TIGG RVLYS GLSLLILKQQ GITSLQFQSL KEISAGNIYI TDNSNLCYYH TINWTTLFST INQRIVIRDN RKAENCTAEG MVCNH LCSS DGCWGPGPDQ CLSCRRFSRG RICIESCNLY DGEFREFENG SICVECDPQC EKMEDGLLTC HGPGPDNCTK CSHFKD GPN CVEKCPDGLQ GANSFIFKYA DPDRECHPCH PNCTQGCNGP TSHDCI UniProtKB: Receptor tyrosine-protein kinase erbB-4 |
-Macromolecule #2: Receptor tyrosine-protein kinase erbB-2
Macromolecule | Name: Receptor tyrosine-protein kinase erbB-2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: receptor protein-tyrosine kinase |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 66.888008 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: QVCTGTDMKL RLPASPETHL DMLRHLYQGC QVVQGNLELT YLPTNASLSF LQDIQEVQGY VLIAHNQVRQ VPLQRLRIVR GTQLFEDNY ALAVLDNGDP LNNTTPVTGA SPGGLRELQL RSLTEILKGG VLIQRNPQLC YQDTILWKDI FHKNNQLALT L IDTNRSRA ...String: QVCTGTDMKL RLPASPETHL DMLRHLYQGC QVVQGNLELT YLPTNASLSF LQDIQEVQGY VLIAHNQVRQ VPLQRLRIVR GTQLFEDNY ALAVLDNGDP LNNTTPVTGA SPGGLRELQL RSLTEILKGG VLIQRNPQLC YQDTILWKDI FHKNNQLALT L IDTNRSRA CHPCSPMCKG SRCWGESSED CQSLTRTVCA GGCARCKGPL PTDCCHEQCA AGCTGPKHSD CLACLHFNHS GI CELHCPA LVTYNTDTFE SMPNPEGRYT FGASCVTACP YNYLSTDVGS CTLVCPLHNQ EVTAEDGTQR CEKCSKPCAR VCY GLGMEH LREVRAVTSA NIQEFAGCKK IFGSLAFLPE SFDGDPASNT APLQPEQLQV FETLEEITGY LYISAWPDSL PDLS VFQNL QVIRGRILHN GAYSLTLQGL GISWLGLRSL RELGSGLALI HHNTHLCFVH TVPWDQLFRN PHQALLHTAN RPEDE CVGE GLACHQLCAR GHCWGPGPTQ CVNCSQFLRG QECVEECRVL QGLPREYVNA RHCLPCHPEC QPQNGSVTCF GPEADQ CVA CAHYKDPPFC VARCPSGVKP DLSYMPIWKF PDEEGACQPC PIN UniProtKB: Receptor tyrosine-protein kinase erbB-2 |
-Macromolecule #3: Betacellulin
Macromolecule | Name: Betacellulin / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 5.630513 KDa |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) |
Sequence | String: GHFSRCPKQY KHYCIKGRCR FVVAEQTPSC VCDEGYIGAR CERVDLFY UniProtKB: Probetacellulin |
-Macromolecule #9: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 9 / Number of copies: 3 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Support film - Material: GRAPHENE OXIDE | ||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 293 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Average electron dose: 45.8 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.9 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |