axonemal A tubule inner sheath / cilium-dependent cell motility / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / axoneme assembly / axonemal microtubule / cilium organization / flagellated sperm motility / nucleoside-diphosphate kinase / UTP biosynthetic process ...axonemal A tubule inner sheath / cilium-dependent cell motility / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / axoneme assembly / axonemal microtubule / cilium organization / flagellated sperm motility / nucleoside-diphosphate kinase / UTP biosynthetic process / CTP biosynthetic process / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / positive regulation of cell motility / regulation of neuron projection development / ciliary base / microtubule organizing center / mitotic cytokinesis / sperm flagellum / axoneme / cilium assembly / alpha-tubulin binding / phosphatase binding / microtubule-based process / cytoplasmic microtubule / Hsp70 protein binding / acrosomal vesicle / mitotic spindle organization / meiotic cell cycle / cell projection / Hsp90 protein binding / structural constituent of cytoskeleton / microtubule cytoskeleton organization / mitotic spindle / mitotic cell cycle / microtubule cytoskeleton / microtubule binding / spermatogenesis / vesicle / microtubule / cytoskeleton / hydrolase activity / calmodulin binding / neuron projection / ciliary basal body / cilium / GTPase activity / centrosome / calcium ion binding / GTP binding / positive regulation of transcription by RNA polymerase II / ATP binding / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能
Coiled-coil domain-containing protein 81 / : / Testicular haploid expressed repeat / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Testis-expressed protein 33 / CFAP97 domain-containing protein 1/2 / Testicular haploid expressed gene protein-like / : / : ...Coiled-coil domain-containing protein 81 / : / Testicular haploid expressed repeat / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Testis-expressed protein 33 / CFAP97 domain-containing protein 1/2 / Testicular haploid expressed gene protein-like / : / : / Testicular haploid expressed repeat / Normal lung function maintenance, Low in Lung Cancer 1 protein / Domain of unknown function (DUF4562) / Testis-expressed sequence 33 protein family / Ciliary microtubule inner protein 3 / Sperm microtubule inner protein 1 / Repeats in THEG (testicular haploid expressed gene) and several fly proteins. / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Sperm microtubule inner protein 8 / Sperm microtubule inner protein 11 / Protein of unknown function DUF1143 / Protein of unknown function DUF4513 / Hemingway/CFA97 / : / Cilia- and flagella-associated protein 68 / Hemingway/CFA97 / Sperm-associated microtubule inner protein 10 / Protein of unknown function DUF4541 / Coiled-coil domain-containing protein 105 / Cilia and flagella associated protein 90 / CCDC81, HU domain 1 / Protein of unknown function DUF4586 / CCDC81, HU domain 2 / : / CCDC81 eukaryotic HU domain 1 / Cilia-and flagella-associated protein 96 / CCDC81 eukaryotic HU domain 2 / Sperm-tail PG-rich repeat / FAM183 family / Sperm-tail PG-rich repeat / FAM183A and FAM183B related / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Protein CFAP95 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / : / Tektin family / Protein of unknown function (DUF3695) / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Nucleoside diphosphate kinase, active site / Nucleoside diphosphate kinase (NDPK) active site signature. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily 類似検索 - ドメイン・相同性
Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Cilia- and flagella-associated protein 97 / Sperm microtubule inner protein 1 C-terminal domain-containing protein / Uncharacterized protein / Ciliary microtubule inner protein 2A-C-like domain-containing protein / Uncharacterized protein / Flagellar FliJ protein / TEPP protein ...Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Cilia- and flagella-associated protein 97 / Sperm microtubule inner protein 1 C-terminal domain-containing protein / Uncharacterized protein / Ciliary microtubule inner protein 2A-C-like domain-containing protein / Uncharacterized protein / Flagellar FliJ protein / TEPP protein / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 126 / Cilia- and flagella-associated protein 45 / Meiosis-specific nuclear structural protein 1 / Uncharacterized protein / Tubulin beta chain / Cilia- and flagella-associated protein 52 / Protein phosphatase 1 regulatory subunit 32 / Coiled-coil domain-containing protein 81 / Cilia- and flagella-associated protein 161 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Coiled-coil domain-containing protein 105 / Outer dense fiber protein 3 / RIB43A-like with coiled-coils protein 2 / Uncharacterized protein / Meiosis-specific nuclear structural protein 1 / Uncharacterized protein / Trichohyalin-plectin-homology domain-containing protein / Uncharacterized protein / EF-hand domain-containing protein / Uncharacterized protein / Testicular haploid expressed gene protein-like / Enkurin domain-containing protein / Tubulin alpha chain / Uncharacterized protein / Uncharacterized protein / Cilia- and flagella-associated protein 53 / EF-hand domain-containing family member C2 / Uncharacterized protein / Ciliary microtubule inner protein 2C / Outer dense fiber protein 3 / CFA20 domain-containing protein / Nucleoside diphosphate kinase / Tektin / Uncharacterized protein / Cilia- and flagella-associated protein 77 / Cilia-and flagella-associated protein 96 / Uncharacterized protein / Uncharacterized protein / Tektin / Flagellar protofilament ribbon protein rib74 / Tektin 類似検索 - 構成要素
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
1R01GM138854
米国
引用
ジャーナル: Cell / 年: 2023 タイトル: Structural specializations of the sperm tail. 著者: Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / ...著者: Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / Tzviya Zeev-Ben-Mordehai / 要旨: Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule- ...Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level.