EMDB-17187: 48-nm repeat of the native axonemal doublet microtubule from bovi...

Basic information

Entry

Database: EMDB / ID: EMD-17187

• Title: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm

Sample

• Complex: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm
  • Protein or peptide: x 66 types
• Ligand: x 3 types

• Keywords: microtubule / cilia / sperm / axoneme / STRUCTURAL PROTEIN

Function / homology

Function:

axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / manchette / sperm axoneme assembly / protein polyglutamylation / inner dynein arm assembly / regulation of calcineurin-NFAT signaling cascade / positive regulation of feeding behavior / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / cilium-dependent cell motility / COPI-dependent Golgi-to-ER retrograde traffic / COPI-independent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / MAP kinase tyrosine/serine/threonine phosphatase activity / RHO GTPases activate IQGAPs / cerebrospinal fluid circulation / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / RHO GTPases Activate Formins / cilium movement involved in cell motility / regulation of store-operated calcium entry / Transferases; Transferring phosphorus-containing groups / MHC class II antigen presentation / acrosomal membrane / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / ciliary transition zone / cilium movement / axoneme assembly / Aggrephagy / sperm DNA condensation / calcium ion sensor activity / The role of GTSE1 in G2/M progression after G2 checkpoint / axonemal microtubule / Separation of Sister Chromatids / cilium organization / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Recruitment of NuMA to mitotic centrosomes / gamma-tubulin ring complex / flagellated sperm motility / Regulation of PLK1 Activity at G2/M Transition / Hedgehog 'off' state / UTP biosynthetic process / CTP biosynthetic process / determination of left/right symmetry / Neutrophil degranulation / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / positive regulation of cell motility / histone H2AXS140 phosphatase activity / RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity / RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity / MAP kinase serine/threonine phosphatase activity / calmodulin-dependent protein phosphatase activity / myosin phosphatase activity / AMP binding / regulation of neuron projection development / ciliary base / beta-tubulin binding / spermatid development / cerebral cortex cell migration / microtubule organizing center / regulation of cell division / mitotic cytokinesis / sperm flagellum / cellular response to UV-C / axoneme / cilium assembly / glial cell projection / single fertilization / alpha-tubulin binding / phosphatase binding / intercellular bridge / cytoplasmic microtubule / 3'-5' exonuclease activity / sperm midpiece / protein tyrosine phosphatase activity

Domain/homology:

Protein of unknown function DUF4542 / Uncharacterized protein C1orf100 / Sperm microtubule associated protein 1 / Family of unknown function (DUF5530) / Testicular haploid expressed repeat / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 / Testis-expressed protein 33 / Testis-expressed sequence 37 protein / CFAP97 domain-containing protein 1/2 / Testicular haploid expressed gene protein-like / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / : / : / : / Testicular haploid expressed repeat / Normal lung function maintenance, Low in Lung Cancer 1 protein / Domain of unknown function (DUF4562) / Spermatid-specific manchette-related protein 1 / TSC21 family / Testis-expressed sequence 33 protein family / Sperm-associated microtubule inner protein 5 / Ciliary microtubule inner protein 3 / Sperm microtubule inner protein 1 / Repeats in THEG (testicular haploid expressed gene) and several fly proteins. / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Sperm microtubule inner protein 8 / Sperm microtubule inner protein 11 / : / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Hemingway/CFA97 / Cilia- and flagella-associated protein 68 / Hemingway/CFA97 / Sperm-associated microtubule inner protein 10 / Protein of unknown function DUF4541 / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Tektin bundle interacting protein 1 / Cilia and flagella associated protein 90 / DJBP, EF-hand domain / Protein of unknown function DUF4586 / : / EF-hand domain / Cilia-and flagella-associated protein 96 / Sperm-tail PG-rich repeat / FAM183 family / Sperm-tail PG-rich repeat / FAM183A and FAM183B related / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Protein CFAP95 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / Sperm acrosome-associated protein 9 / : / Tektin family / Protein of unknown function (DUF3695) / Sperm acrosome-associated protein 9 / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 161-like domain / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / : / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile.

Component:

Ciliary microtubule inner protein 3 / Piercer of microtubule wall 2 protein / Cilia and flagella associated protein 77 / Cilia and flagella associated protein 90 / Sperm microtubule associated protein 2 like / Sperm microtubule inner protein 11 / Sperm acrosome associated 9 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 1 / Protein SPMIP9 / Parkin coregulated gene protein / Tektin-3 / Sperm-associated microtubule inner protein 5 / Cilia- and flagella-associated protein 144 / Sperm-associated microtubule inner protein 10 / Sperm microtubule inner protein 8 / Enkurin / Cilia- and flagella-associated protein 276 / Sperm microtubule associated protein 1 / Cilia- and flagella- associated protein 210 / Cilia- and flagella-associated protein 52 / EF-hand domain-containing protein 1 / Sperm-associated antigen 8 / EF-hand domain-containing family member B / Tektin bundle interacting protein 1 / non-specific serine/threonine protein kinase / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 161 / CFAP97 domain containing 1 / Outer dense fiber of sperm tails 3 / Ciliary microtubule inner protein 2A / Sperm microtubule inner protein 1 / EF-hand calcium binding domain 6 / Dual specificity phosphatase 21 / Meiosis-specific nuclear structural protein 1 / Protein SPMIP2 / Cilia-and flagella-associated protein 96 / EF-hand calcium-binding domain-containing protein 3 / Cilia- and flagella-associated protein 68 / Tektin-2 / Stabilizer of axonemal microtubules 4 / Cilia- and flagella-associated protein 107 / Tektin-4 / Tektin-5 / Tektin-like protein 1 / Tubulin alpha-3 chain / Sperm microtubule inner protein 6 / Tektin-1 / Protein SPMIP3 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 95 / Ciliary microtubule inner protein 4 / RIB43A-like with coiled-coils protein 2 / Cilia- and flagella-associated protein 45 / Piercer of microtubule wall 1 protein / Tubulin beta-4B chain / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase homolog 7 / Cilia- and flagella-associated protein 20

• Biological species: Bos taurus (domestic cattle)
• Method: single particle reconstruction / cryo EM / Resolution: 3.6 Å
• Authors: Leung MR / Zeng J / Zhang R / Zeev-Ben-Mordehai T

Funding support

Netherlands, 1 items

Organization	Grant number	Country
Netherlands Organisation for Scientific Research (NWO)	740.018.007	Netherlands

• Citation: Journal: Cell / Year: 2023; Title: Structural specializations of the sperm tail.; Authors: Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / Tzviya Zeev-Ben-Mordehai / ; Abstract: Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level.

History

Deposition	Apr 21, 2023	-
Header (metadata) release	Nov 22, 2023	-
Map release	Nov 22, 2023	-
Update	Nov 22, 2023	-
Current status	Nov 22, 2023	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_17187.map.gz / Format: CCP4 / Size: 1.1 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.041 Å

Density

Contour Level	By AUTHOR: 0.2
Minimum - Maximum	0.0 - 2.2935586
Average (Standard dev.)	0.013966969 (±0.0784226)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 672 672 672 Spacing 672 672 672
Cell	A=B=C: 699.552 Å α=β=γ: 90.0 °

Supplemental data

Additional map: #1

• File: emd_17187_additional_1.map

Half map: #2

• File: emd_17187_half_map_1.map

Half map: #1

• File: emd_17187_half_map_2.map

Sample components

Entire : 48-nm repeat of the native axonemal doublet microtubule from bovi...

• Entire: Name: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm

Components

• Complex: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm
  • Protein or peptide: Cilia- and flagella-associated protein 95
  • Protein or peptide: Sperm acrosome associated 9
  • Protein or peptide: Testis specific serine kinase 6
  • Protein or peptide: EF-hand domain-containing protein
  • Protein or peptide: ATP6V1F neighbor
  • Protein or peptide: Outer dense fiber of sperm tails 3 like 1
  • Protein or peptide: Outer dense fiber of sperm tails 3
  • Protein or peptide: Tubulin alpha-3 chain
  • Protein or peptide: Tubulin beta-4B chain
  • Protein or peptide: Protein Flattop
  • Protein or peptide: Cilia- and flagella-associated protein 53
  • Protein or peptide: Nucleoside diphosphate kinase 7
  • Protein or peptide: EF-hand domain-containing family member C2
  • Protein or peptide: Family with sequence similarity 166 member A
  • Protein or peptide: Protein FAM166C
  • Protein or peptide: Outer dense fiber of sperm tails 3B
  • Protein or peptide: Spermatid-specific manchette-related protein 1
  • Protein or peptide: Enkurin
  • Protein or peptide: Coiled-coil domain-containing protein 105
  • Protein or peptide: Protein phosphatase 1 regulatory subunit 32
  • Protein or peptide: RIB43A-like with coiled-coils protein 2
  • Protein or peptide: Cilia- and flagella-associated protein 107
  • Protein or peptide: Piercer of microtubule wall 1 protein
  • Protein or peptide: Piercer of microtubule wall 2 protein
  • Protein or peptide: Stabilizer of axonemal microtubules 1
  • Protein or peptide: Stabilizer of axonemal microtubules 3
  • Protein or peptide: Meiosis-specific nuclear structural protein 1
  • Protein or peptide: Tektin-3
  • Protein or peptide: Tektin-5
  • Protein or peptide: Sperm-associated antigen 8
  • Protein or peptide: Spermatogenesis-associated protein 48
  • Protein or peptide: Tektin-1
  • Protein or peptide: Tektin-2
  • Protein or peptide: Uncharacterized protein C1orf100 homolog
  • Protein or peptide: TEPP protein
  • Protein or peptide: Uncharacterized protein MGC137036
  • Protein or peptide: Testis expressed 33
  • Protein or peptide: Testis-expressed sequence 37 protein
  • Protein or peptide: Testis-expressed protein 43
  • Protein or peptide: Testis expressed 49
  • Protein or peptide: Theg spermatid protein like
  • Protein or peptide: EF-hand domain-containing family member B
  • Protein or peptide: Tektin bundle interacting protein 1
  • Protein or peptide: Tektin-4
  • Protein or peptide: Chromosome 7 open reading frame 31
  • Protein or peptide: Cilia- and flagella- associated protein 210
  • Protein or peptide: EF-hand domain-containing protein 1
  • Protein or peptide: Uncharacterized protein C10orf82 homolog
  • Protein or peptide: Cilia- and flagella-associated protein 144
  • Protein or peptide: Chromosome 20 C5orf49 homolog
  • Protein or peptide: Chromosome 19 C17orf98 homolog
  • Protein or peptide: Chromosome 13 C20orf85 homolog
  • Protein or peptide: Cilia- and flagella-associated protein 68
  • Protein or peptide: Cilia and flagella associated protein 77
  • Protein or peptide: UPF0602 protein C4orf47 homolog
  • Protein or peptide: Cilia- and flagella-associated protein 20
  • Protein or peptide: Parkin coregulated gene protein
  • Protein or peptide: CFAP97 domain containing 1
  • Protein or peptide: Cilia- and flagella-associated protein 45
  • Protein or peptide: Cilia- and flagella-associated protein 52
  • Protein or peptide: Cilia- and flagella-associated protein 141
  • Protein or peptide: Cilia- and flagella-associated protein 161
  • Protein or peptide: Dual specificity phosphatase 21
  • Protein or peptide: Uncharacterized protein C4orf45 homolog
  • Protein or peptide: Cilia- and flagella-associated protein 276
  • Protein or peptide: EF-hand calcium-binding domain-containing protein 3
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: 48-nm repeat of the native axonemal doublet microtubule from bovi...

• Supramolecule: Name: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#66
• Source (natural): Organism: Bos taurus (domestic cattle)

Macromolecule #1: Cilia- and flagella-associated protein 95

• Macromolecule: Name: Cilia- and flagella-associated protein 95 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 26.661025 KDa

Sequence

String:

MDSSDSSCQE WCSHGQYWLE LGPTDLMERK GSLTLRSHHK KYSKPVLVYS WHRDRETYPK DYDIEGPEEV KKLCNSTYRR LGTSEPPVW ISETREKMAQ VCLNTKLAKI KSKALLNEET MNSGIIERDT GLPATGFGAL FTRHSPDWSK MCTLTTYAEE Y APPYEYQP LGDPCQDDDY SIVHRKCRSQ FTDLDGSKRV GINTWHDESG IYANSYVKRK LYSLTGGPIA PFLK

UniProtKB: Cilia- and flagella-associated protein 95

Macromolecule #2: Sperm acrosome associated 9

• Macromolecule: Name: Sperm acrosome associated 9 / type: protein_or_peptide / ID: 2 / Number of copies: 29 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 25.174801 KDa

Sequence

String:

MMNEVKESLR SVEQKYKIFQ QQQFTFIGAL EHCRENAHDK IRPISSIGQV QSYMEHHCSN STDRRILLMF LDICSELSKL CQHFEALHA GTPVTNNLLE KCKTLVSQSN DLSSLRAKYP HDVVNHLSCD EARNHYGGVV SLIPIILDLM KEWVAHSEKL P RKALQQVS EPQAATRATA HAPQASGTQP QLRKQNCGQL IQNIPKPGGK DQGSSKPPWR PPGGKL

UniProtKB: Sperm acrosome associated 9

Macromolecule #3: Testis specific serine kinase 6

• Macromolecule: Name: Testis specific serine kinase 6 / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 30.256893 KDa

Sequence

String:

MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP DFVNKFLPRE LSILRGVRHP HIVHVFEFIE VCNGKLYIV MEAAATDLLQ AVQRNGRIPG GQARDLFAQI AGAVRYLHDH HLVHRDLKCE NVLLSPDERR VKLTDFGFGR Q AHGYPDLS TTYCGSAAYA SPEVLLGIPY DPKKYDVWSL GVVLYVMVTG CMPFDDSDIA GLPRRQKRGV LYPDGLELSE RC KALIAEL LQFSPSARPS AGQVARNGWL RAGDSG

UniProtKB: non-specific serine/threonine protein kinase

Macromolecule #4: EF-hand domain-containing protein

• Macromolecule: Name: EF-hand domain-containing protein / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 31.349949 KDa

Sequence

String:

MPVNSKGRLR YLDFLSSFNV DKQATPLATS DSAKAQRGSS VPEVSDRGRS AGSSPTRDPK AGSKPRSHPC TPSSVSGTPP LQNCEPIET KLRKKVQGCW RELLRECKER DFNKQGEIPG PEFLALVEKF NLDISRDECQ QLLIKYDLKN NGKFAYCSFI Q SCVLLLKA KETSLMQRMK IQNANKMKEA GAETCSFYSA LLRIQPKIVH CWRPMRRTFK AYDEGGTGLL SVADFRKVLR QY SINLSEE EFFHILEYYD KTLSSKISYN DFLRAFLQ

UniProtKB: EF-hand calcium binding domain 6

Macromolecule #5: ATP6V1F neighbor

• Macromolecule: Name: ATP6V1F neighbor / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 20.279707 KDa

Sequence

String:

MSRQLSMDTV RQNFWKEEYL REKMLRCEWH HKYGSLVKAK QKAKAAARLP LKLPTLPPKA PLSPPPTPKA VPSRASSPAL EAPIQSEMY PVPPATRALL YEGISHDFQG RYRYLSTRKL DVPEKRYLFP ITTNFTYGWQ LGPPVKQELV SCKMCRIESF F RKNGAFSL LDPRDLAL

UniProtKB: Sperm microtubule inner protein 1

Macromolecule #6: Outer dense fiber of sperm tails 3 like 1

• Macromolecule: Name: Outer dense fiber of sperm tails 3 like 1 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 30.860854 KDa

Sequence

String:

MKLPKGVKNP VFYGQQPEKK VPMSSGHEIK QTPVVLAMLK GPGPAKYLRP SCTGYIDHDV SMFQEPAYTL HAWHPEKRIM DIRSPGPCY FLDPKITRFG MVSCPQVPMA EHISNLRLSP TPAPCHYHLE KTHLPGERRA PGYSFGFRCP YRVMDPNPGP N RYQMPLLL GPNLPANKAA PCYSLAPLDK NWFYKNMSRG PGPAAHTRPE PSVYQNRSPV YSMAKRFGYP VDHTPRPGPG SH NVQQVRA HKPRPPAFTM GIKHSPHLCP LIVDIRD

Macromolecule #7: Outer dense fiber of sperm tails 3

• Macromolecule: Name: Outer dense fiber of sperm tails 3 / type: protein_or_peptide / ID: 7 / Number of copies: 14 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 27.684936 KDa

Sequence

String:

MAEEVWVGTW RPHRPRGPIM ALYSSPGPKY LIPPTTGFVK HTPTKLRAPA YSFRGAPMLL AENCSPGPRY SVNPKILRTG KDLGPAYSI LGRYHTKTTL TPGPGDYFPE KSTKHVFDSA PSHSISARTK TFRVDSTPGP AAYMLPMVMG PHTIGKVSQP S FSIKGRSK LGSFSDDLHK TPGPAAYRQT DVQVTKFKAP QYTMAARVEP PGDKTLKPGP GAHSPEKVTM TRPCAPVVSF GI KHSDYMT PLVVDVD

UniProtKB: Outer dense fiber of sperm tails 3

Macromolecule #8: Tubulin alpha-3 chain

• Macromolecule: Name: Tubulin alpha-3 chain / type: protein_or_peptide / ID: 8 / Number of copies: 149 / Enantiomer: LEVO; EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 49.978344 KDa

Sequence

String:

MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVVDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIVDLVL DRIRKLADLC TGLQGFLIFH SFGGGTGSGF ASLLMERLSV D YGKKSKLE FAIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCM LYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKLD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GVDSVEAEAE EGEEY

UniProtKB: Tubulin alpha-3 chain

Macromolecule #9: Tubulin beta-4B chain

• Macromolecule: Name: Tubulin beta-4B chain / type: protein_or_peptide / ID: 9 / Number of copies: 153 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 49.877824 KDa

Sequence

String:

MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEAEEEVA

UniProtKB: Tubulin beta-4B chain

Macromolecule #10: Protein Flattop

• Macromolecule: Name: Protein Flattop / type: protein_or_peptide / ID: 10 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 21.214035 KDa

Sequence

String:

MATNYSANQY EKPFSPKYLQ NWSLAKPTKE RISSHEGYTQ IIANDRGHLL PSVPRSKASP WGSFMGTWQM PLKVPPARAT LTSRTAAGA ASLTRWIQKN PDLLKASNGL RPEIFGKPHD PDSQKKLRKS ITKTVQQAPS PTIIPSSPAS NLSSPDQLQS S HPSAGHTP GPQSPLNSPK CPPGSPCLPH AGRNLAEV

Macromolecule #11: Cilia- and flagella-associated protein 53

• Macromolecule: Name: Cilia- and flagella-associated protein 53 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 62.138992 KDa

Sequence

String:

MYSQRFGIVQ REVKGPTPKV VIVRAKPPKG QGAEHHLQRI QHSHQKHHAI LASIKSIERD RLKTEWDQHN DCKFVDSLVK ARVKDAMQG FIINTEERRN KLRELLASEE NEYFTEMQLK EETIEEKKDR MRDKIRLLRE KKEKERQDFV AEKLDQQFRE R CQELRAEL FCIHQKAVCE ERKAQIAFNE ELKRQKVVEE QMFSKLWEED RLAKERREAK EERRQKELVE NTRLGLNAQV TS IQAQRQA AQRLKEEEAL LVENENAQVK LENEQDKLKK QKTKQEIRAA LQKALQEKME RMQQEYREEQ DLNMKLMQNA LQS LQEETD KKKQKKEDMR REQKIYYQYL AQRHEEEKAQ EKELDRMLEK EKEKKFAEKD KELRLEKEAR KQLLNEVMCT RKLQ VQEKL QRKAKEQEER TMEQERINEG LKELNCEERE NFIRRCSLAQ EYRKQLQMQI CSQQQAREAE EEEERREFEA GIAAE KSFQ DKIQGILSTH QVVPRNIHPM RRACSTKLPP

UniProtKB: Cilia- and flagella-associated protein 53

Macromolecule #12: Nucleoside diphosphate kinase 7

• Macromolecule: Name: Nucleoside diphosphate kinase 7 / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 42.650895 KDa

Sequence

String:

MNHSERFVFI AEWFDPNASL FRRYELLFYP GDGSVEMHDV KNHRTFLKRT KYEDLHLEDL FIGNKVNIFS RQLVLLDYGD QYTARQLGS KKEKTLALIK PDAVSKAGEI IEIINKAGFT LTKLKMMTLS RKEATDFHID HQSRPFLNEL IQFITSGPII A MEILRDDA VCEWKRLLGP ANSGLARTDA PESIRALFGT DGIKNAAHGP DSFACAAREM ELFFPSSGVC GPANTAKFTN CT TCCIVKP HAVSEGLLGK ILMTIRDAGF EISAMQMFNM DRINVEEFYE VYKGVVSEYN EMVTEMYSGP CVAMEIQQTN PTM TFREFC GPADPEIARH LRPGTLRAIF GKTKIQNAVH CTDLPEDGLL EVQYFFKILD N

UniProtKB: Nucleoside diphosphate kinase homolog 7

Macromolecule #13: EF-hand domain-containing family member C2

• Macromolecule: Name: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 13 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 85.566766 KDa

Sequence

String:

MGKEKFHKSQ HWGFCNNVRM LVGEDKPGIG GELLFGQKIK PKYSVFPKGM GTDSPSWVAF DKQVLSFDAY LEDEVPDKSQ ENYRIRRYK IYFYLEDDTV EVNEPVLQNS GLPQGIFIRR HRISLPPPNE DQFYTVHHFN VNTDIVFYGR TFKVYDCDAF T KNFLTKIG VKLNPPGQCP EDPYMKTRRE KLDCMEPICP YESFDTLKQF LEYDRKVLRF FCVWDDSGSV FGDRRELILH YF LSDDTIE IKEVLPHNSG RDAMSLFLQR RKLPKYGPPG VYQPGQLTDQ TVLNVYGGYS ETRVYGFLLD KYQLGKLDQE FYK DTDLSI GTTINVWGRK VLLCDCDDFT KTYYRTKYGI ENFTSIPCKA PSPPKIERKF PPYTGFGSEE DSLRSCIGLM PTPH QRNFK KFIEFDSYGN ISNTLRFFAK LITHKCADVE RMFVISYFLS DDTISVFEPI ERNSGYTGGM FLKRVRVKKP GQEVF KSEF SEYIKAEELY VGAKVNVNGY LFFLVNADEY TLNYMERNSD KFPLSSIELV IQKLKEEECK SRELKQVFTA ADCMHT KMV DFNTFREIMM NLTVGKLTDQ EVITIARRYR VPEDPCPHRN VLVAQAHEQL KKNAFENFER LIAMCVYEDR EKKKVLP SK DIKRLCKSSR LPLNEDLLGS LLSGFEDSEK QINYESFFCA LNWRVNPVPV LEVASYIKER CEDEWLGMPS PIPVKYIY Y LNLLKDVFGV DE

UniProtKB: EF-hand domain-containing family member C2

Macromolecule #14: Family with sequence similarity 166 member A

• Macromolecule: Name: Family with sequence similarity 166 member A / type: protein_or_peptide / ID: 14 / Number of copies: 14 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 36.251363 KDa

Sequence

String:

MTAPQKHNLF SPEPHYIPGY AGFYPQLRYR VGDTYGRTTA QLLTDPSVQK SPCSVLAPVA KPKFIEDFSK PKPPFVPCRD LIEPYIPHY TGLKPYKNFE MLGRFPPQEA DAQGSLGGEN VSRQVPLPAG FMPYPPYAPC PPGRKGDSRD LGHPGLRLAL G EEAWKSTA PACEAPGQYQ LYHCRRDESP PLAHWQETLD VGRFHRLPQL GHPKLIQRKA ISGYAGFVPR FAWVMGMNYR DG VTQAMDE FDKSQFLLRN PICALGERLP STHWPSNTIY RSQGLIPFYM GFIPSMQDNY ALTFGNSTRK AYQKELERRS RTL

UniProtKB: Ciliary microtubule inner protein 2A

Macromolecule #15: Protein FAM166C

• Macromolecule: Name: Protein FAM166C / type: protein_or_peptide / ID: 15 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 23.414553 KDa

Sequence

String:

MASRSAGTLL TEFNAAYVPP GLMPGYKGHV PGVAFSFGSP YGTTTLKYFQ DQRNAALGRS STAFSRGGHF PTIFSLNPTQ VLRNRALTR DRWLHTPSYT RFNLDSSRWA ELLHFYQMAQ RHREHYQDKT GLVHRVPYFV LPVKEWDRYP IPTDLPPLSP K EKWHLLRV APENLRTYQT FPSGKRVSPQ ERQRRDCYFE FRA

UniProtKB: Ciliary microtubule inner protein 2C

Macromolecule #16: Outer dense fiber of sperm tails 3B

• Macromolecule: Name: Outer dense fiber of sperm tails 3B / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 32.579918 KDa

Sequence

String:

MGSGSWALQW WGSRHPTLPS QPGDTGTGVA GRKRSHRAMG SDVWVGPWRP HRPRGPIGAL YRGPGPKYKL PPSTGYIMHD PSRPRAPAF TFGARLPAQQ TSCGPGPGHL VPARMTVRGL DSAPAYSIFG RPRHAAPFLT PGPGRYFPER AGNAAYPSAP R HTIAPRNW GLRLESQTPG PGTYTMPSLL GPRVVGKVSA PNYSIYGRSA VGSFCEDLSK TPGPCAYHAV NPGVYKPRAP QF SMLPRTS FPQGNTLNPG PAAYNVDQHR KPRGWTFGIR HSDYLAPMMT EADGDPLGGR CAADVS

Macromolecule #17: Spermatid-specific manchette-related protein 1

• Macromolecule: Name: Spermatid-specific manchette-related protein 1 / type: protein_or_peptide / ID: 17 / Number of copies: 10 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 35.109043 KDa

Sequence

String:

MFLFSRKTKT PISTYSDSYR APTSIKEVYK DPPLWAWEAN KFVTPGLTHT AQRHVDPDAL QKMLKCAVQD YSYKGSIPSH PYFPEKYWL CPEEADRCNP NYLCGNPNYL CSNQYNTWRM GPYNCWNKCT TYLPRLPKEA GMETVVRGMP LVYPPKPERL N AYEREVVV NMLNSLSRNQ PLPQITPRCG CVDPLPGRLP FQGYESACSG RHYCLRGMDY CVSGPPCTER RLRPLCTELP TV RSVSPCE HRSGMQCAVI TPQPSYYPCP NLRWDTSHFK KTGGSQRNNY VVHPEFVSET YPDYHCW

UniProtKB: Sperm microtubule inner protein 6

Macromolecule #18: Enkurin

• Macromolecule: Name: Enkurin / type: protein_or_peptide / ID: 18 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 30.215129 KDa

Sequence

String:

MVAMDPTGPS ESIYNLIPSD WKEPPQPPRY VSIFKATVKD DMQKFKTAMK TMGPAKLEVP SPKDFLKKHS KEKILPPKKK FEWNERRKP PVPLRTDHPV MGIQSEKNFI NTNAADVIMG VAKKPKPIYV DKRTGDKHDL ETSGLVPKYI NKKDYGVTPE Y ICKRNEEV KKAQEEYDNY IQENLRKAAM KRLSDEEREA VLQGLKKNWE EVHKEFQSLS VFIDSIPKKI RKQKLEEEMK QL EHDIGVL EKHKVIYIAN KK

UniProtKB: Enkurin

Macromolecule #19: Coiled-coil domain-containing protein 105

• Macromolecule: Name: Coiled-coil domain-containing protein 105 / type: protein_or_peptide / ID: 19 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 57.747836 KDa

Sequence

String:

MPVLIPPVEH SQDTRVGHPA WREATRALAE EAHQLTDRCG QEAVTMWQPN ESVRDPHVAH HLCRAAYILP WRFRVEMLKG GSTMEKPPP GEGVTLWKSK MKPPAWHARL PLPMHRDARA LQTAEVVQAH ARGARLTAAR LGRAQHQING QLRLLQRQRE A TDRRLSEV RKALLINQQS VKLRGYRPKS ETISDKADSM LTWEKEELKS MKRKMEADME KSEALLKTLA SCRDALVFCC KE RLQAVEL MNQPLDKVLE QAGRHSWVNI SRVPTPRTQG LKTPPPDPVG TYTPECAKAL YEAKRLLMES KDTLLDMAKN EED IREQQQ QISDRVCASL AQKMRETLEL KDRLNMTLGL MRGTIHRCTK FNQEMYITRG LIKGPLSKSH LETREKLDRP LVRM YQRHV GTQLPEAARL AQGTDKLQRH ISHVEKNLDE LLSMRKKLTW SFNCKKIGHN VDYSVVRLRL RQRHPQVCYE QAQRL VNDW DPRTPPRVKS NTAITK

UniProtKB: Tektin-like protein 1

Macromolecule #20: Protein phosphatase 1 regulatory subunit 32

• Macromolecule: Name: Protein phosphatase 1 regulatory subunit 32 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 47.818125 KDa

Sequence

String:

MMGKLPLGVV SPYVKMSSGG CTDPLKFYAT SYCTAYGRED FKPRVGSHRG TGYKSNYRPV VFYQPHLDAL DNTATGEQGC NNFQTVTSQ SYRPLEVPDG THPLPWNLHQ TNSGYSREKA SAVTPIKEVR KVHFDTQDYG PQAITGLEPK DAPLLHQQQN K GSLEWENA GHGPRFMTSE YNSKYLKEPS HQPDLLQKNS VGAKEETGFT EESNKNPIVF QPPSQALPGD PVLLPGRSVT KS DFLPISH PHGDEFLPVL ARGSERETGF SRVNERTLNP RVPPPCPEPS SMNHWQFQSP QRMQQTNVAL LGRETVGNKE PSG FSLNNP SYVRSPYDPD MDNRYLTTYN QGYFENIPKG LDREGWTRGG LQPQKPGGYA LNQPVTRLEA TPTPTESLRR LHPH LGRTL ISVDPFYRTA PPSGHVSRFT APN

UniProtKB: Stabilizer of axonemal microtubules 4

Macromolecule #21: RIB43A-like with coiled-coils protein 2

• Macromolecule: Name: RIB43A-like with coiled-coils protein 2 / type: protein_or_peptide / ID: 21 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 44.773418 KDa

Sequence

String:

MEVAQPKDLK EDFVLAKRRH AELVRQKRIF NARNRIIGGD TTAWDAQVCD QNIKAATEKA RDEAFAAEMR QNDKIACLSE NRERRDRKN LCKAINDFQQ SFQRPETRRE FDLSDPLALK KDRPARQSDY DARNTISGMQ KFMGEDLNFH LRKKFQEEQN R EWSLQQQK EQMIGRENQK CAEDLYLKTR LQFDETAKHL QNLETATRKA VCATVKEFNK NQALESAEKK IQERKQEQED NL AEISNML RGDLLSENPQ QAASSFGPHR VVPDRWKGMS QEQLEEIRLV QRQQVQEKLR LQEEERQRDM DWDRRRIQKA RAT LLFEQQ QQRLQRGLRR ALDCSNLSLA REQLLQKKHM KELCTNHATE DYFTQFNTGS R

UniProtKB: RIB43A-like with coiled-coils protein 2

Macromolecule #22: Cilia- and flagella-associated protein 107

• Macromolecule: Name: Cilia- and flagella-associated protein 107 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 23.271453 KDa

Sequence

String:

MQFLTAVSPQ SSSTPSWKIE TKYSTRVLTG NWTEERRKFI KATEKTPQTI YRKEYVPFPG HRPDQISRWY SKRTVEGLPY KYLITHHQE PSQRYLISTY DDHYNRHNYH PGLPELRTWN RHKLLWLPEK ADFPLLGPPT NYGLYEQLKQ KWLPPPEATL R ESIYTSSY PRPPAGAMSR REHAIPVPPP RLQPVPHF

UniProtKB: Cilia- and flagella-associated protein 107

Macromolecule #23: Piercer of microtubule wall 1 protein

• Macromolecule: Name: Piercer of microtubule wall 1 protein / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 15.673458 KDa

Sequence

String:

MSEEDPKACA EPEEPKAGPP PEKTSDWYRV SEDLPARFNN PAWFRGYRTK EPPSVYRTSN QAYGSRAPTV HEMPKVFYPN SYKFSRQVA VGGMFQNNTL NVYMEKSIVT GPDNYITSYD RLNFHPSYRV CRPSICD

UniProtKB: Piercer of microtubule wall 1 protein

Macromolecule #24: Piercer of microtubule wall 2 protein

• Macromolecule: Name: Piercer of microtubule wall 2 protein / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 13.784531 KDa

Sequence

String:

MTECDWEKKS TSASNSDTEM KPELPPCVNP GNPVFSCMLD PKTLHTTTSL SKPKMIMYKT NSSNYGEFLP MPQFFPCYYT PREQVFSSH IRATGFYQNN TLNTAPDRTR TLDFPNFQHT L

UniProtKB: Piercer of microtubule wall 2 protein

Macromolecule #25: Stabilizer of axonemal microtubules 1

• Macromolecule: Name: Stabilizer of axonemal microtubules 1 / type: protein_or_peptide / ID: 25 ; Details: MAPTKGKCVCELCSCGRHHCPHLPTKIYDKTEKPCLLSEYTENYPVYHSYLPRESFKPKM DYQRACTPMEGLTTSRRDFGPHKVLPVKIHQPNPFVPSEENMDLQTTYKQDYNPYPLCRV DPFKPRDSKYPCGDKMESLPTYKADYLPWNQPRRELLRPPHHYRPASTKFDSRTTQQDDY SMKGLVNTRSCKPPAVPKLCNVPLEDLTNYKMSYVAHPLEKRFVHESEKFRPCEIPFESL TTHKESYRGLMGEPAKSLKPPARPYGLDTPFSNTTEFRDKYQAWPTPQVFSKPPSMYVPP EEKMDLLTTVQTHYTYPKGAPAESCRPALSVKKGGRFEGSTTTKEDYKQWASTRTEPAKP IPQLNLPTEPLDCLTTARAHYVPHLPMMTKSCKPVWSGPQGNIPVEGQTTYTISFTPKEM SRCLASYPEPPGYIFEEIDALGHRIYRPVSQTGSRRSSRFSVGDSENPNQQELTVSA; Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 40.613059 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #26: Stabilizer of axonemal microtubules 3

• Macromolecule: Name: Stabilizer of axonemal microtubules 3 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 37.465422 KDa

Sequence

String:

MAGRTLALRY GPPWSPISGT EVPGSWPNWH LTSSGVAHHF IPPVSFPPPT VQSTVAEPLP PASKQDLHIW AFDEVISRWE TTSGSALVP KTHGGPYAQP KTPEPANPTR AVGIKDLGEK LRHRGWRLPL ITKHQCSETK AQYGGWPDLD RGPTSYFGPQ P LELADHHR GGPSQALIPW TKNPELAGRP FTISDRGILD RHQLYLTTSA RDFRPYSKKE SSSYPCKDSM TSNFGETPQA GG HSQKQLP CPSSSRPPQR AKIRLPRGRP VIPVVPHRGA LTLAQESYNL PLHPLRRLDR FCPLELPWGG PHWKPVSGIY SVP HAYRTE NSNYGSLKPA LV

Macromolecule #27: Meiosis-specific nuclear structural protein 1

• Macromolecule: Name: Meiosis-specific nuclear structural protein 1 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 60.538367 KDa

Sequence

String:

MASIRRTLSF SERHQKLVDI NYCKKLHVEA LQRLQNQTRD QMVQNENDDR AERKRFLRLL QDEQFELDME EAIQKAEENK RLRELQLAQ EEKLATELAK LKRESLKDEK LRQQVRENSA ELRELEKKLK AAYMNKERAA QIAEKDAIKY GQMKRDAEIA R TMMEEHER LIKEESAAED KRNQAKAQYS HDLEKQLEEQ GKKKQEAYEQ LLKEKLMIDE IVRKIYEEDQ LERQQRLEKM NT TRRYIEE FQKEQALWRK KKREEMEEEN RKIIEFAKLQ QQREEDRMAK VQEKVKKKRL QLKNMLTQRL EEMLRQREDL EQV RQELYQ EEQAEIYKKK LEEEAEEKLR KQKELKQDFM DQMALKELIL QAAKEEEETF RKAMLAKFAE DDRIELMNAQ KPRM KQLEH KRAVEKLIEE RRNQFLADKQ RELEEWQWQQ RRQGCINAIV EEERLKLLKE HATKLLGYLP KGVFKNEDDI DMLGE EFRK AYQKRSEICE K

UniProtKB: Meiosis-specific nuclear structural protein 1

Macromolecule #28: Tektin-3

• Macromolecule: Name: Tektin-3 / type: protein_or_peptide / ID: 28 / Number of copies: 15 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 56.76091 KDa

Sequence

String:

MELLGSTLTA TYAHPRPTPT NFLPAISTMA STYRDRFPHY NLTHSLSLPW RPSTYYKAAS NWPTLDPYCT RSQRVSESTM LPFVSNRTT LFTRYTPDDW YRSNLTNFQE SNTSRHNSER LRVDTSRLIQ DKYQQTRKTQ ADSTQNLGER VNDIGFWKSE I IHELDAMI GETNELTDIK KRLERALMET EAPLQVAREC LFHREKRMGI DLVHDEVEKE LLTEVDTILC CQERMKLYLD KA IAQLAAN RAAQHELEKD LSDKQSAYRI DDKCHHLRNT SDGVSYFHGV ERVDATVSVP ESWAKFTDDN ILRSQSERAA SAK LRDDIQ NVLVVTANEM WNQFNKVNLA FTNRIAETAD AKNKIQTHLA KTLQEIFQTE MTIESIKKAI VEKSAFLKVA QTRL DERTR RPNIELCRDM AQLRLVNEVY EVDDTIQTLQ QRLRDAEDTL QSLAHTKATL EHDLAVKANS LYIDQDKCMS MRRSF PSTL RLVGFC

UniProtKB: Tektin-3

Macromolecule #29: Tektin-5

• Macromolecule: Name: Tektin-5 / type: protein_or_peptide / ID: 29 / Number of copies: 26 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 56.564809 KDa

Sequence

String:

MEFLGTTQTA TYCGPKKPCG GSVLPSVASG PTVQECYQPY YLPGYRYLNA WRPSLFYKVA NAQTCLDEGA TCRSALRPPT ILPAVRSAL FCRYSPQDWD QSNRLQLCGA EASRLWAGRM TGDSMRLMQD KDQLTRQMQE GTCRNLGQRL SDIGFWKSEL N YELERLLN ENHSLDAIKR RLECAAEELR CPLQVALECL YQREKRIGID LVHDNVEKNL IREVDMIKCC QEQMRKLAQR ID IQMRDNR DAQHALERDL EDKNSAEFID EKCLNLRNTS DCISFFHGVE KIDGTISVPE TWAKFSNDNI RHSQNMRANS IRL REEAQN LFDVLADQLW RQFTNTNLAF NARIAEETDV KNKLQTQLAK TLQEIFQTEN TIMLLERSIM AKECPLKVAQ TRLE CRTRR PNVELCRDSP QFKLVNEVFT IDDTLQTLKL RLRETRDTLQ LLVMTKCRLE HELAIKANTL CIDKEKCMAM RKTFP STPC MGICP

UniProtKB: Tektin-5

Macromolecule #30: Sperm-associated antigen 8

• Macromolecule: Name: Sperm-associated antigen 8 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 51.604398 KDa

Sequence

String:

METSESTDRS QSRCLDLQPS SDGLGSSSDP FSSWDGRHRS ALVAATAAAS AAATAASTAR AAALWTKSPA PYSHGNLLTE PSSDSLTER YTGPRFTHKI SHGRLGFQPA YFSHIAWNPY TTNDLSSSRG PIPGSSSGPV PGSSSSPGPD SSSDPGPSSS S GPGGSPGG SGRGPGHGPG PGGGSGQGPG GGSGQGTDLG PAIDSRHSPG HGHGPRFNFS APVGFRNPRG DLIPNYTGCK HH CHWEPQK QSWKFLKVSE PGARGLWKPP EVEGKSTVLS ETLPRGQCLL YNWEEERATN YLDQVPVMQD GSESFFFRHG HRG LLTLQP QSPTSSCTTQ KDSYQPPKSH CQPIRGKREA ILEMLLRQQI CKEVQAEQEP TRKDSEVESV THHDYKKELV QAGP PAPTK IHDYHTEQPE TFWLERAPQL PGVSNIRTLD TPFRKNCSFS TPVPLSLEQP LPFEPESYSQ HGEISSLACQ GGGQG GGGG

UniProtKB: Sperm-associated antigen 8

Macromolecule #31: Spermatogenesis-associated protein 48

• Macromolecule: Name: Spermatogenesis-associated protein 48 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 49.735164 KDa

Sequence

String:

MDTYSQLIPG KISISEGSFL SRTKAVENID YPHYCALLRK LNMPFVKGVE DRHDYGRMEK KCNPTFLKFH PYPPSVLPDY HLHYPYPPP YGPDYPLFPL RDDVPLQDPC SGFLSPGGDA DLKPGVGRTI PSLVDFSDVK PQHRVPRPDT GFQTTLKRKT I LLEELKQD RRWNSRAVPD ISIRARLGGW TSPLKVTPSQ PHHGEHSINH PYIFDEEATC TDNGEPVLQS NKKYSAKDSF YK SSTQKAY EMVPWDKMLP PKLDPEETTV EKAADPVSHC FTLKRYEGLP AITQMVGGLW DRFQTRSFLA PVKPVNFVSP SSR SKYIPL YTGCVQSTNA DDIDNPCGDI TSLAKPRSSE ILYTNSSRSA NIPGYTGKVH FTATHPANSD RPSTTPSPDS EVHH VLRKR MAVDLFRHQG PLSRMVTTVT PYNPFNKKDK ETISY

Macromolecule #32: Tektin-1

• Macromolecule: Name: Tektin-1 / type: protein_or_peptide / ID: 32 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 48.798375 KDa

Sequence

String:

MAKLLQQPPK FLRAEWQIAN KNQYHRAEAQ RSRSERLVAE SQRLVDEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVYATE DLLLYQTRLQ KALESFKEPL HITEKCLEYR EKRVGIDLVH DEVEQELIKE HEIIRGVMTL LTRTLEETCE Q IRLNRSAK YNLEKDLRDK FTAITIDDIC FSLNNNSPNI KYSENVVRVE PNSVSLEDWL DFSNTNVEKA DKQRNNSLTL KA LVDRILF QTASDLRRQC DVVDTAFKNG LKETKDARDK LALHLDKVME EIASQEKNIV VLEKAILDQE GPAKVAHTRL ETR THRPNV ELCRDVAQYR LIKEVDEITH NVARLKETLA QAHVELKGLN RRQLALQEEI QIKENTIYID EVLCVPMRKS IPPR DGDDH GEWAGGSHPE AVC

UniProtKB: Tektin-1

Macromolecule #33: Tektin-2

• Macromolecule: Name: Tektin-2 / type: protein_or_peptide / ID: 33 / Number of copies: 9 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 49.962656 KDa

Sequence

String:

MATLSVKPSP RFRLPDWQTN SYLLSTNAER QRDASHQIRQ EARVLRNETN NQTIWDEHDN RTRLAERIDT VSRWKEMLDK CLTDLDAEI DALAQMKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV KDPVEEELHK EVEVIEATKK ALQQKISQAF E KLFLLQEA RQRLNSDHRG KMETLDIDRG CLSLNLTSPN ISLKINPTRV PNGSTSLQQW DDLSRFNKDH GEAEMKKAIE LR EAIALTI AETNNELEAQ RVATEFAFRK RLREMEKLYS ELKWQEKNTL EEIAELHEDI RHLEEDLRRK LQNLKLCHTR LEA RTYRPN VELCRDQAQY GLTDEVHQLE ATIAALKQKL AQAQDALDAL YKHLARLQAD IACKANSMLL DTKCMDTRRK LTVP AEKFV PEVDTFTRTT NRTLSPLKTC QLELA

UniProtKB: Tektin-2

Macromolecule #34: Uncharacterized protein C1orf100 homolog

• Macromolecule: Name: Uncharacterized protein C1orf100 homolog / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 16.642066 KDa

Sequence

String:

MTAIRLREFI DRRPAIPPSI FIVHQGKDLR GYYTGQLARV HYDYSVKRSP RPLIDLDIPF KKKSQYQPQL DQQTLIQYIC FRRRSKPAE PWYKETSYQR DYSLPFYKTD WDRKLATVSS NPRPLNSLPE HYCCEGRWL

UniProtKB: Protein SPMIP3

Macromolecule #35: TEPP protein

• Macromolecule: Name: TEPP protein / type: protein_or_peptide / ID: 35 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 24.939441 KDa

Sequence

String:

MARIIDLVPW EDGSTHVYAS PAILLPIPRR RNQLAGVKQQ LYHPALPSLR RMDMDSVKAC LSDEHCQSTT YCRKDDFDNA YFTLLGVPN KPLQCLDITE TGQRLRNRYH EGKLAPIAPG INRVDWPCFT RAIEDWSRFV SSAGEFKLPC ASKKVESFSG Y AVRYLKPE VTQSWRFCLN QNPSLDRYGQ KPLPFDSLNA FRRFGSNYSR VNYLTPWH

UniProtKB: Sperm microtubule inner protein 8

Macromolecule #36: Uncharacterized protein MGC137036

• Macromolecule: Name: Uncharacterized protein MGC137036 / type: protein_or_peptide / ID: 36 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 21.238109 KDa

Sequence

String:

MLLAEEFAES ALHRRVLFHA AAGRAGAEGG PSLPRLPCSG LDSAPSPRGP LPGHAPRRPP RPRTGRQVLG APPLSEKCEL PGQDSQKSS VPSHGPKTPS GQKVKAPHRP LSLSWKQDRE QTLAAAYVPV VVDPRGQNPE KLRFNFYTSQ YSNSLNPFYT L QKPTCGYL YRRDTDHTRK RFDVPPANLV LWRS

UniProtKB: Ciliary microtubule inner protein 3

Macromolecule #37: Testis expressed 33

• Macromolecule: Name: Testis expressed 33 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 30.322604 KDa

Sequence

String:

MELGHRPGTT TLTRAHPNNN KEGQQDTDPW RAAHSPLDTS KLKYQAPVSV KPSLYRGDSP RSSSLGRASL EEIPPPPPSA VSRGSLWRD SDPESLKKGS FRPSSRESRA SLREGAQPCQ APKEDPNAGA QGQSGSSIPN NIRHKFGSNV VDQLVSEEQA R KAIGEALE GQKRTSSWPS RIQSPTEITS IFSDYYDLGY NMRSNLFQGA PQETKSLMKA SYTPEVIEKS VRDLEHWHGR KT DDLGRWH QKNAMNMNLQ KALDEKEKSK NKNSK

UniProtKB: Ciliary microtubule inner protein 4

Macromolecule #38: Testis-expressed sequence 37 protein

• Macromolecule: Name: Testis-expressed sequence 37 protein / type: protein_or_peptide / ID: 38 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 20.375061 KDa

Sequence

String:

MSGVVFPGQA PVDLDIYQSS YMIDYRPYGK HKYARVTSEE QAKLDTQLQD KEFYRPTPSP NPKLEDGYPA FKRPHMTAKD LGQPGFFPP QGRVGPVEDE WRFTSTCPSV YPASHALYLA HGDPNRIQQS ADFPCLLEPE HQPAPDVGKG YFLLPGCACT Y HCTVKVPI LNRWGPLMPF YQ

UniProtKB: Protein SPMIP9

Macromolecule #39: Testis-expressed protein 43

• Macromolecule: Name: Testis-expressed protein 43 / type: protein_or_peptide / ID: 39 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 15.209745 KDa

Sequence

String:

MASGKDTCPI LPKLTNNCSD ENSDKLANKC EIHLPRFSLK QGMIPRHYVM PWKQNMKFRN VNLKHAEACG IHAGPLEDSL FLDHSERLC HGEDRKVVLK KGPPEIKIAD MPLHSPLSRY QSTVISHGFR RRLI

UniProtKB: Sperm-associated microtubule inner protein 10

Macromolecule #40: Testis expressed 49

• Macromolecule: Name: Testis expressed 49 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 15.441421 KDa

Sequence

String:

MAFFNLHLLG YQNSFRSKKR DKTEETNQKD PVPTRLPPIF SEDGNYSVHQ NSHTKYHEAV RKVSLKTFPN QVFRVPLTDA QNFSFWRSN AAGARPEETM PWIQNPRHCL IKSAMTRFMD HSILNDRNFS LY

UniProtKB: Sperm microtubule inner protein 11

Macromolecule #41: Theg spermatid protein like

• Macromolecule: Name: Theg spermatid protein like / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 50.941473 KDa

Sequence

String:

MESQEFSGPS GLSDGHHTAE TSARSEALQK PLVRIFDMNK QLEESEEAGF SEESEEIPNH PKAYELYQSY ESHKPLKPHA PYEPHAPRE VRKPREPRKP RGPPVPREPH KSQEIELLPR AGVMVSPSLL MRLPPRIPPS SLSGRCWGLS GALSNPTPSR P GGSNGKVC LSFSRKRIQD LSRPKKQWRA PDRKLFWGNQ DPIRPVSRNA LKAQMTQRLE NLAQSKEVSH RYVPNRVQYY YS CGRESVI WEIPSPALFC QPSKRIQKLA QPNRSKRGDY LIRGSLQFSD PSPRILRLSI AKATNPNYVP PKSIETKILS STL TAIAAP RIVDLAHPRI KIEGLCFERE RSEKPIRPIS HAALLAHPSP RILALAKAKP LHKDYLPPRD ALWPVSYAAI HSKI SPRIQ ELANPNTRTP VHIVYYDPEV FKVKPAALKT QCSPRIQELA EPITR

UniProtKB: Sperm microtubule associated protein 2 like

Macromolecule #42: EF-hand domain-containing family member B

• Macromolecule: Name: EF-hand domain-containing family member B / type: protein_or_peptide / ID: 42 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 97.960547 KDa

Sequence

String:

MCSFLRVPSP KGLEPSKGLG APLQPGTAYP SEMSALGPGM TVSGRKSPLS MEVGLAPEGK DDLEERRVFM GTTSPTELGL RVGLRKDFP CSKTYEERMA SPEIRSPSSK GLELRLKRQN ISRTVMDGSN LGVHRVSASQ ETKPPLSALP GRVGLEKERF L AGYCPGRV TQPPLGQGCE SPQASGGRRC PVTGDPEGFG ASVSKLPALG MECRGELGGE SACVVMKPRA ETEPPVEVDM GL TRLEEPA EMESPEPQMG LVMEPPAWQL AQQPEEQREA ENTEPGVEPP DRIRPIYSGK FFDRMPCWPS AGKVLPIGYR AAT CLTERF PRLMTPPEAK KFFNFRYPPA GAERVFYGRA NDPQIAPSLT HGIRSKISIP AKVLINPQPI TTFQQKMKDK KESV YFSNQ RAPLGKSHDQ TPGLPKGLDI LNTTFGTAIV RETSARDMVN PPKPYKEVFE EAQAGHDLYV VSHNDYFVGE AKNRK YDPS SFHRFNLYGI PTPHFNDGRN MAKTLHWLHE LQMKRGAKIV SKRVDDFKEK FQHRLGRVLD PIAETMNVPP DYTFGA FLR PEDYGVGDLI HRRLPGEYLR GKDRQRGLVA AVRHHLKKVN YQNFDTLLAA FRHYDKKGDG VIDRAELQEA CDQACLH LD EKLLDQLFEY CDVDKDGLIN YLEFANFLTW KDKTPLKEYE ERVLIKGRKA DCANPAEANV EESEPALLLK PEDIVLKE P GSSEKTLRTL LRPSDKVSNH YKTTSSEISA VVGAVPSTCY PTYGVPTIRS DIPAPLIRRV SDRTSYGEEG NAYSLLHPT IFAQKGVFER DFFKTRSKQE ISEILCNIGV KLSEDEFENV WNLASKKHHR GEVCVENIRS VLDELQHADG AKCKAAT

UniProtKB: EF-hand domain-containing family member B

Macromolecule #43: Tektin bundle interacting protein 1

• Macromolecule: Name: Tektin bundle interacting protein 1 / type: protein_or_peptide / ID: 43 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 24.559482 KDa

Sequence

String:

MQTLRRQAAW PCVPRGTLEV DFPPPLYSDD YLSQEGPRWT PAIKQATRWK YTPMGRDAAG QLWYTGLTNS DSREAWYTLP RAPDSPYRE AYARWHGCYG HRERSLPSAY TQRLRETAWY DPIIPAQYTD PSTRWGSVLW KDRPIRGKEF AINRHRFGVE P LWRASDYV RYLSAPQRPR YTAQNYRQWG LEPYCPATNQ RPPPVYTPSH

UniProtKB: Tektin bundle interacting protein 1

Macromolecule #44: Tektin-4

• Macromolecule: Name: Tektin-4 / type: protein_or_peptide / ID: 44 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 52.025488 KDa

Sequence

String:

MAQTDILLTK EPAPQTVPAC QLPRKLYDVA RNTGAHTSSG LATSGFRTAK YLLDEWFQNC YARYHQAFAD RDQSERQRHE SQQLAAETE ALAQRTQQDS TRKVGERLQD MHGWKSELQR QVEELVSETE LLLAQKQRLE RALDATAGPF SIVTDNLQCR E RRQHPDLV RDCVEIELLK EAELIRNIQE LLKRTIKQAV SQIRLNWEHK ETCEMDWSDK VEAYNIDEAC CRYNNQSTDV QF YPHSAKF EESASTPETW AKFTQEHLYR AERERLASVN LRNLIDCILQ DTSEDLRLQC DAVNLAFGRR CEELEDARHK LEH HLRKTL REISDQEHNI AALKQAIKDK EAPLKVAQTR LYQRSHRPNV ELCRDAAQFR LASEVEELNL SLAALKEKLL EAEQ SLRNL EDTRMSLEKD IAIKTNSLFI DRHKCMAHRA HYPTVLQLAG YQ

UniProtKB: Tektin-4

Macromolecule #45: Chromosome 7 open reading frame 31

• Macromolecule: Name: Chromosome 7 open reading frame 31 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 67.68557 KDa

Sequence

String:

MEVIHGQPYC CRELEGADIL SDTFYSNELH TPLETATRPT ASEDRYQKFR QSLQRCRLPW GAEREYGGMI PISLPEEHRP KCEPPRVMG KGHQHYGFGG EIWPRKLPIE QFYYLSQNKK SDIYGNDSLL PKPPNSTVGE ICSPYPIEHP YHTHISRGAM F PTFTSPKD LYTGIKARTQ QPFPPTVPTK PYDTTVLKTR GNPYRYELLD FPMDSKKKAL VWPGQRVYFD LPKCVEKNKP VF YPKPPKT FAPNTSLNSW DPITSLKEVN IQRNLEKSHW ITSYNHDFTG LGPMNPLELD DYHEKEVAEL TGQIGFDPEP QEK VHPALK PTRPLEGRIA RLIQNQRPLE AILEQRPSSC PDCTPRVLCT FHTFVPSSTE MMALSDNIPA DVTHKNQEIE EKIK EEQSL LSTYALPSCY PTKDLANTYD IKPFPKITDT KKTEDLYWRQ LSLKPQLIPY CNPDHYIPYE HLNQYNVYQN PVSLS KPGI LQSKPDLKTF DFEHFLSKPE QLTLNMEDDE ETKPILGWIP RAGVAKPQTD LLELKNAFSK TGAQKRFHKS VLEDYK DLR DKEHLGKKHQ FYGHNSYYFY N

Macromolecule #46: Cilia- and flagella- associated protein 210

• Macromolecule: Name: Cilia- and flagella- associated protein 210 / type: protein_or_peptide / ID: 46 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 65.437598 KDa

Sequence

String:

MLVRFGRRSG QAKESAEMKN CEEDPVLYPP LLPTKVDLRQ VTIIPHNEWE RIRDSLDSLT REAACLRAER KAKKEMHLRS QEVVKHWTN TYAGMKEQKL EAKKKRDEEI EAERQILDIE EAIYKQGERK KAIELAKQYQ FYQTERVKNF HSGLLLSRVM K ERDAQIEF QKSKIKSDKK WEEQVKLNVE KAFKEEREKA EKQRRERVAL AKDHLKQIKE HEEEEERRRK EEEKDAEEIK RQ NSLYEIE MKKKQGKKKE EINESRRLFF EHLNDKHIIK AVEQQQQEEE DEKIRKFIKA KKRLTQMGKE KEAETHRLME ERR KRINNF LSKLMKEKFD NEDLIIARDI AEAEAEWEKR EREKYEKNKA ELKAIAEHRA LVMKNKEEEE RQRKIEATEQ MLAI LKADQ IFWEHEKEKK QKADKERREV QDAHIQQMAK HKFNALQAKQ AESEYCRLTE ALVAEKEKEF QDYAREVIES ESEST KKYI YPLVKAVQEG PGGGRGPVLV DRGGLRPSYQ ANDTTGVQLP FYNSPGSKYN NFQKSKGRLG FTW

UniProtKB: Cilia- and flagella- associated protein 210

Macromolecule #47: EF-hand domain-containing protein 1

• Macromolecule: Name: EF-hand domain-containing protein 1 / type: protein_or_peptide / ID: 47 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 74.125344 KDa

Sequence

String:

MVSNPVHGLP FLPGTSFKDL TKTAFHRSQT LGYRNGYAVV RRPTVGIGGD RLQVNQLSQA DLDELASKIP ILTYGQARQA PPAAFVPAH VAFDKKVLKF DAYFQEDVPM SIEEHYRIRQ VHIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRLSKNDR G DHYHWKDL NRGINITIYG KTFRIVDCDK FTQVFLESQG IELNPPEKMA LDPYTELRKQ PLRKYVTPTD FDQLKQFLTF DK QVLRFYA IWDDTDSMFG ECRTYIIHYY LMDDTVEIRE VHERNDGRDP FPLLMNRQRM PKVLVENAKN FPRCVLEISD KEV LEWYTA KDFIVGKPLT ILGRTFFIYD CDPFTRQYYQ EKFGISDLPR IDMSKKEPPP MKQELPPYNG FGLIEDSAQN CFAL IPKAP QKDVIKMLMN ENKVLRYLAT LESPFPEDKG RRFVLSYFLA TDMISIFEPP VRNSGIIGGK YLGRTKVVKP GSSVE NPVY YGPSDFFIGA VIEVFGHRFV ILDTDDYVLK YMESNAAQYS PEALLSIQNH IRKQEAPAPE LDGQQAEEDP GVRDLE ALI DTIQKQLKDR PCRDNIREAF QIYDKEASGY VDRETFFKIC GSYQLPVDDS LIKELIRMCS HGEDKIDYYN FVRAFSN

UniProtKB: EF-hand domain-containing protein 1

Macromolecule #48: Uncharacterized protein C10orf82 homolog

• Macromolecule: Name: Uncharacterized protein C10orf82 homolog / type: protein_or_peptide / ID: 48 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 26.677043 KDa

Sequence

String:

MESSKTFMRQ MPITPGYSGF VPYLSCQGTS SEDNMANCLK IFQENTRRCK DQLEEFHCSV ATAPKLKPVR SEGTVLRTLH QYYRQYHPL SLECKNIKKP LHEPPIPGWA GYLPRARVTE LGCSTRYTVM ARNCYRDFLD LMERAKQAHL KPYEKIYGVK S TPPPPTPP PKVLLHEGLL PKYPDFSIPG ARCPTLSRAL MEDPRPLMTC GCPQRPSVWC SGKIYLEPLS GGKDMEG

UniProtKB: Sperm-associated microtubule inner protein 5

Macromolecule #49: Cilia- and flagella-associated protein 144

• Macromolecule: Name: Cilia- and flagella-associated protein 144 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 16.178324 KDa

Sequence

String:

MAGHQKEKAI ADEVHQNQIL RELYLKELRT QKLYTQYHVN PLRKVHTIAR KPMSWHDNLE EPADARFLNL IHHAAQGPRK KYPETQTEG QEIGWDSEPL VNPQRDDRRL NHFRVYKDIT LYKAKMWSLG EDDRHK

UniProtKB: Cilia- and flagella-associated protein 144

Macromolecule #50: Chromosome 20 C5orf49 homolog

• Macromolecule: Name: Chromosome 20 C5orf49 homolog / type: protein_or_peptide / ID: 50 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 16.820848 KDa

Sequence

String:

MEDDEEEITA AALRGKPRPL PISALSAFSY IPPRRQDPKE HSYYYRQGWT GIISLYDCVF KRDPGYNQKL HRDDREHAKS LGLRINEEE CERPVGVLSS SVYGRRIDQP VEPLNRDHGR AGHVKADFYR KNDITSIKAP GFGHISPA

UniProtKB: Cilia and flagella associated protein 90

Macromolecule #51: Chromosome 19 C17orf98 homolog

• Macromolecule: Name: Chromosome 19 C17orf98 homolog / type: protein_or_peptide / ID: 51 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 17.813178 KDa

Sequence

String:

MAHLCACPLR REKGFILDGV AVSTMARSYE HLRPKLWSAI PPYNAQQDCH SRRYFRSRVV PPILRETDQD HGGTGRDGWI VDYFHIFGQ GQRYLNRRNW AGAGHSLQQV TGHDHYNTNL KAIQGFNGRF GYRRNTPALR HCPSVFGEIT RFPLF

UniProtKB: Sperm microtubule associated protein 1

Macromolecule #52: Chromosome 13 C20orf85 homolog

• Macromolecule: Name: Chromosome 13 C20orf85 homolog / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 15.810196 KDa

Sequence

String:

MAQKPISTAA AERMNLVAQD EIWKYRLKAE NEARQNWAQN WGFLSTPFEE LIKCEEEPAT PKPKIELPER FRIRPVTPVE KYIKVLPSP PVPKTTQGFI GWRSGVPGLN KCLEHDYEIR SCKGAYAKEL NWPKQGIH

UniProtKB: Ciliary microtubule inner protein 1

Macromolecule #53: Cilia- and flagella-associated protein 68

• Macromolecule: Name: Cilia- and flagella-associated protein 68 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 20.16326 KDa

Sequence

String:

MTATDYHCYS EFFQRQVLAC FLTNPHYGSL INADGHAEVW TDWNDMSKFF QYGWRCNTNE DAYSNRTLMG NWNQERYDLK NIVQPKPLP SQFGHYFETT YDTSYNNRRP LSTHRFKREP HWFPGHQPEL VPPPYKCTEK STYMTSYSKP QTDHHSVCVW N PSNCQFQS P

UniProtKB: Cilia- and flagella-associated protein 68

Macromolecule #54: Cilia and flagella associated protein 77

• Macromolecule: Name: Cilia and flagella associated protein 77 / type: protein_or_peptide / ID: 54 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 32.999203 KDa

Sequence

String:

MPEARNSCQD LTRWKKKKPP VRRTVSQICP PPRRPLTVAD IRPGMENERL GVVRDSMFQN PLIVKAELGK PRERSCSLPG INFNYGLYI RGLDGGVPEA IGHWNVFKQQ PTCPHELTRN YIAMNRGAVK AGLVTARENL HYRQLNDIRI SDQDDRRLKK E PPSLPPNM TFGIRARPST PFFDLLQHRY QQLWVQEQKD TQKAIKLEKK HKVILGKLYE TRSSQLRKYK PPMGLDTLWH MP HFQKVGP HLATFPTEAD RQRAFKAHRE ECAVRQGTLR MGNYTHP

UniProtKB: Cilia and flagella associated protein 77

Macromolecule #55: UPF0602 protein C4orf47 homolog

• Macromolecule: Name: UPF0602 protein C4orf47 homolog / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 34.554371 KDa

Sequence

String:

MPAEGGKTDM ERIGLFSEME YITVGDKYVS QFNRPFNESA SKNRQILPGG SKEMSNLQAG YFDPHFVRIF EGESYVNPNQ VRRRYMMEE AKKNLSKAFL PSNGEKKPCG LGSYYGTIGG PVPFFSAQSK PKEKYEPPGK NLYTNPGKKG TGYGYANITI G KQFSHSSD LYDAAKLNNK KENEEHRRLL KGTAFKLNLY PREYFDTNPY MSEKPLPPIK KVEKKETVGN PFKPSSPGKK AG GMKAGTF DPYPSHSADP YVVKLKSPSS KSAKVFHPPG GPKSRPIESI MALNVKRALN MKNYKTASVQ SY

UniProtKB: Cilia-and flagella-associated protein 96

Macromolecule #56: Cilia- and flagella-associated protein 20

• Macromolecule: Name: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 56 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 22.781389 KDa

Sequence

String:

MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

UniProtKB: Cilia- and flagella-associated protein 20

Macromolecule #57: Parkin coregulated gene protein

• Macromolecule: Name: Parkin coregulated gene protein / type: protein_or_peptide / ID: 57 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 29.323059 KDa

Sequence

String:

MVAEKETLSL NKCPAKLPTR TKLLAQQPLP VHQPHSLVSE GFTVKAMMKN SVVRGPPSAG AFKERPTKPT AFRKFYERGD FPIALEHDS KGNKIAWKVE IEKLDYHHYL PLFFDGLCEM TFPYEFFARQ GIHDMLEHGG NKILPVIPQL IIPIKNALNL R NRQVICVT LKVLQHLVVS AEMVGEALVP YYRQILPILN IFKNKNVNSG DGIDYSQQKR ENIGDLIQET LEAFERYGGE DA FINIKYM VPTYESCLLN

UniProtKB: Parkin coregulated gene protein

Macromolecule #58: CFAP97 domain containing 1

• Macromolecule: Name: CFAP97 domain containing 1 / type: protein_or_peptide / ID: 58 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 19.600385 KDa

Sequence

String:

MNNSLDYLAY PVIVSNHRQS TSFRKKLDFG HYAFHKNRIQ IVKPTVDTKP PAAHTHHILK LSKLQGEQKR IDKIEYENKQ LCQKIANAH RGPAKVDCWN EYFSKSLNRE TRNRELVRIT VENQGILKRL GDRKPHYDRK SSELDWQNSR RYIRNTTRYL L SRDK

UniProtKB: CFAP97 domain containing 1

Macromolecule #59: Cilia- and flagella-associated protein 45

• Macromolecule: Name: Cilia- and flagella-associated protein 45 / type: protein_or_peptide / ID: 59 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 65.800328 KDa

Sequence

String:

MPLSTAGVLS SASTASNRSR NRPRYRTKAL SSEVDESLFG AIKPLTRSDS PIVLLRDKHA IRKTLTALGL DHKPETIQLI TRDMVRELI IPTKDPSGQS LIMSPEEFER IKWASHVLTK EELEAREQAF KKEKEAIVDT VTTRKKIMKQ KEMVWRNNRK L SDLEEVAK ERAQNLLQRA NQLRMEQEEE LKDMKKIILN AKCHAIRDAQ ILEKQLIQKE LDAEEKRLDQ MMEVERQKSV QR QEELDRK RREERIRGRR HIVEQMEKNQ EERSLLAEQR EQEKEQMLEY MEKLQEEDLR DLEQRHQQKL KMQAEIKRIN DEN QRQKAE LLAQEKLADQ MVMEFTKKKM AREAEFEAEQ ERIRREKEKE IARLRAMQEK AQDYQAEQDA LRAKRNQEVA DREW RRKEK ENAQKKMETE AKLRKSRLEQ VAFKEHTLAV QVQRDRDEFE RILRAQREQI EKERLEEEKK ATGRLQHANE LRRQV RENQ QKQVQARIAT FDEGRRLKEE AQKRRERIED IKRKKLEELR ATGLPEKYCI EAERKANIPV NTSVN

UniProtKB: Cilia- and flagella-associated protein 45

Macromolecule #60: Cilia- and flagella-associated protein 52

• Macromolecule: Name: Cilia- and flagella-associated protein 52 / type: protein_or_peptide / ID: 60 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 68.719602 KDa

Sequence

String:

METQASPKDE VAEAVELELE AVIGFNGHVP TGLKCHPDQE HLVYPLGCTI LIQAINTQEQ NFLHGHGNNV SCVAISKSGL YIASGQVTF MGFKADIILW DYKKRELMAR LSLHKGKIEA LAFSPNDMYL VSLGGPDDGS VVVWSIAKRE AICGSPAAGL N VGNATTVI FSKCRDEMFV TAGNGTIRVW ELDLPNRKIW PTECQTGQMK RIVMSISMAN DDSFFYLGTT TGDILKMNPR TK LLADTGP AKDKFSLGVS AICCLKMGGL LVGSGDGLLV FCKSPSYKPI KKIQLQGGIT SITLRGEGHQ FFVGTEESHI YRV NFTNFK ETLITTCHFE SVEDIVFPFG TAELFATCAK KDIRVWHTLT NRELLRITVP NMTCHGIDFM RDGKSIISAW DDGR IRAFA PETGRLMYVI NNAHRIGVTA IATTSDCKRV ISGGGEGEVR VWHIGHQTQK LEEALKEHKS SVSCIRVKKS NEECV TAST DGTCIIWDLV RLRRNQMILA NTLFQCVCYH PEEFQIITSG TDRKIAYWEV FDGSVIRELD GSLSGAVNGM DITVEG VHF VTGGNDHLVK VWDYNEGEVT HVGVGHSGNI TRIRISPGNQ YIVSVSADGA ILRWKYPFPS

UniProtKB: Cilia- and flagella-associated protein 52

Macromolecule #61: Cilia- and flagella-associated protein 141

• Macromolecule: Name: Cilia- and flagella-associated protein 141 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 12.361437 KDa

Sequence

String:

MSMEKMGKVE EHFQRALELK KMVHRWRNSH THCLWQITLS QRRNPYAILR MQDTMVQELA LANKQLLMVR QAALHQLFEK EHQQYQQEL NEKGKAFYME RL

UniProtKB: Cilia- and flagella-associated protein 141

Macromolecule #62: Cilia- and flagella-associated protein 161

• Macromolecule: Name: Cilia- and flagella-associated protein 161 / type: protein_or_peptide / ID: 62 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 36.519594 KDa

Sequence

String:

MAQNLYGPRV RIGNWNEDVY LEEEIMKDFL AKRDKGQLLI QRNRRLKENL LRPMQLSVSE DGYIHYGDKV MLVSPDHPET EADLFLPGD LSLCMTPDEI KAHLSNELEV PCGLSAAQTK IPVGRNTFTI LCAAGEVIGQ VLRYGQNFRL GITGGFDDRM L YLSSDHRT LLKSSKRSWL QEVFLTHEDS YLNCWQAAFP HPQLRLEYEG SPVPANTKIL ITHCHTNRGL VAHRHLFLRT YF GQEAEVA AHTYLDSHRV EKPKNHWMLV TGAPRKDLST MLDLPKPPAE DTRALEQERE QVSDPGARST PDARGCVPQC TLP M

UniProtKB: Cilia- and flagella-associated protein 161

Macromolecule #63: Dual specificity phosphatase 21

• Macromolecule: Name: Dual specificity phosphatase 21 / type: protein_or_peptide / ID: 63 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 22.075557 KDa

Sequence

String:

MTTPLCPFPI QAVRQPAAHG LSQITKSLYL SDAVAAKDKA MLSTNHITTV INVSMEATDT FLEDIQYVKV PLADAPNSRL YDFFDFIAD HIHSVEMKQG RTLLHCAAGV SRSATFCLAY LMKYHSMSLL DAHTWTKSCR PTIRPNNGFW EQLIYYEFKL F SKNTVHMI SSSMGMIPDV YEKEICLMTL MGVIPDSS

UniProtKB: Dual specificity phosphatase 21

Macromolecule #64: Uncharacterized protein C4orf45 homolog

• Macromolecule: Name: Uncharacterized protein C4orf45 homolog / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 22.640896 KDa

Sequence

String:

MACVTFREPI SATVGKRMVF TGPAYVKDHL PQVAQHTAYI GEKRPALEKT GDLRYLWRPA SNRSLPAKYK PEYVGEIGWG IPEYDFINK TRLQTGFHIK YEEFSQAAID KLSHRYQSPW QPNPSIMDAE GSSSRGFIAW HMGDYEDTSQ RNSKRAVLLR Q SKAALPIG SRPPKLPKLP KKEEKSKFRP LHQHDARCY

UniProtKB: Protein SPMIP2

Macromolecule #65: Cilia- and flagella-associated protein 276

• Macromolecule: Name: Cilia- and flagella-associated protein 276 / type: protein_or_peptide / ID: 65 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 19.387805 KDa

Sequence

String:

MPLTRDPFQN PALDKDDSYL GKSRASKKLP YKNPTHLAQQ QEPWCRLSST PTITSMKRDG FFFYSEIPKD DLDFRLAALY NHHTGTFKN KSEILTHQET IQDTRRIKTQ FPGEFLPAPQ PPLITSRANI RHWINPKKES IHSIQGSIVS PHTAATNGGY S RKNDGGFF ST

UniProtKB: Cilia- and flagella-associated protein 276

Macromolecule #66: EF-hand calcium-binding domain-containing protein 3

• Macromolecule: Name: EF-hand calcium-binding domain-containing protein 3 / type: protein_or_peptide / ID: 66 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 50.207961 KDa

Sequence

String:

MAVSEIKAKF KLNPLTKVPN FHSRRDKDLP GLLPCQLQHK ERKLSPSQMR AFQDAYNFFN KDKTGCIDLH GMMCTLAKLG MNLTKHDVH NELRCADIDQ DGKVNFSDFL KVLTDKNRFL KAVVPEKGTC LDLAGNPGIL LFEILSKLIE TSALPKKAIS E IVSYFRRK FQETTSGMVW SPDTTGYGKR RFKPDICTPP SSSTAAFANA ARIAIMKEKD LFKFLDELKK CNPPSDSPYS KI PIFPLFP NVDGVVMGKP FKDIQKLEML KRKEPLNFFE NYFFHKKDWK TQAANIKPVD PTSGYSTDIL TIDQMLKRKQ NWT VADAAA IKPHVKRATE TYNLGIALEH RKQMLNLWRK IRGDLIGIES KNESFYDTFS TYTWSWNVCQ ELLSPKDLKL YDAH VNRNA LHNSVFSSSS DISECDTDTG RKRKRKGFKG FRQ

UniProtKB: EF-hand calcium-binding domain-containing protein 3

Macromolecule #67: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 67 / Number of copies: 149 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #68: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 68 / Number of copies: 149 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #69: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 69 / Number of copies: 153 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.9
• Grid: Model: Quantifoil / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE-PROPANE / Instrument: HOMEMADE PLUNGER

Electron microscopy

• Microscope: FEI TALOS ARCTICA
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
• Sample stage: Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Talos Arctica / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 532665

Startup model

Type of model: EMDB MAP
EMDB ID:

24664

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 34083
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)