EMDB-39957: Cryo-EM structure of the human ubiquitylated pre-40S ribosome wit...

Basic information

Entry

Database: EMDB / ID: EMD-39957

• Title: Cryo-EM structure of the human ubiquitylated pre-40S ribosome with RIOK3
• Map data: local_resolution filtered map

Sample

• Complex: ubiquitylated pre-40S ribosome with RIOK3
  • RNA: x 1 types
  • Protein or peptide: x 36 types
• Ligand: x 1 types

• Keywords: ubiquitylation / ribosome / RIOK3

Function / homology

Function:

cellular response to dsDNA / negative regulation of MDA-5 signaling pathway / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / caspase binding / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / positive regulation of respiratory burst involved in inflammatory response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / nucleolus organization / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / protein tyrosine kinase inhibitor activity / IRE1-RACK1-PP2A complex / positive regulation of endodeoxyribonuclease activity / positive regulation of Golgi to plasma membrane protein transport / translation at postsynapse / cellular response to dsRNA / TNFR1-mediated ceramide production / positive regulation of innate immune response / negative regulation of DNA repair / negative regulation of RNA splicing / mammalian oogenesis stage / supercoiled DNA binding / activation-induced cell death of T cells / oxidized purine DNA binding / NF-kappaB complex / neural crest cell differentiation / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / U3 snoRNA binding / ubiquitin-like protein conjugating enzyme binding / translation at presynapse / regulation of establishment of cell polarity / rRNA modification in the nucleus and cytosol / positive regulation of ubiquitin-protein transferase activity / negative regulation of phagocytosis / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / laminin receptor activity / negative regulation of peptidyl-serine phosphorylation / protein kinase A binding / preribosome, small subunit precursor / negative regulation of ubiquitin protein ligase activity / pigmentation / Ribosomal scanning and start codon recognition / ion channel inhibitor activity / Translation initiation complex formation / positive regulation of mitochondrial depolarization / positive regulation of T cell receptor signaling pathway / fibroblast growth factor binding / negative regulation of Wnt signaling pathway / positive regulation of activated T cell proliferation / negative regulation of translational frameshifting / monocyte chemotaxis / Protein hydroxylation / BH3 domain binding / TOR signaling / cysteine-type endopeptidase activator activity involved in apoptotic process / SARS-CoV-1 modulates host translation machinery / regulation of cell division / iron-sulfur cluster binding / mTORC1-mediated signalling / T cell proliferation involved in immune response / Peptide chain elongation / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Formation of a pool of free 40S subunits / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / phagocytic cup / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / negative regulation of ubiquitin-dependent protein catabolic process / Viral mRNA Translation / negative regulation of respiratory burst involved in inflammatory response / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / erythrocyte development / Major pathway of rRNA processing in the nucleolus and cytosol / negative regulation of protein-containing complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / spindle assembly / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Protein methylation / ribosomal small subunit export from nucleus / positive regulation of intrinsic apoptotic signaling pathway / negative regulation of canonical NF-kappaB signal transduction / Nuclear events stimulated by ALK signaling in cancer / positive regulation of JUN kinase activity / translation regulator activity / rough endoplasmic reticulum / laminin binding / Mitotic Prometaphase

Domain/homology:

Serine/threonine-protein kinase Rio3 / : / Putative WW-binding domain and destruction box / Putative WW-binding domain and destruction box / Nin one binding (NOB1) Zn-ribbon-like / Ribonuclease Nob1, eukaryote / NOB1 zinc finger-like superfamily / Nin one binding (NOB1) Zn-ribbon like / Ribonuclease, PIN domain / RNA-binding protein NOB1 / PIN domain of ribonuclease / Low temperature viability protein Ltv1 / Low temperature viability protein / RIO kinase, conserved site / RIO1/ZK632.3/MJ0444 family signature. / Tsr1, G-like domain / RIO kinase / RIO-like kinase / RIO domain / : / : / Eukaryotic type KH-domain (KH-domain type I) (327.11.2) / Large family of predicted nucleotide-binding domains / Ribosome biogenesis protein BMS1/TSR1, C-terminal / AARP2CN / Bms1/Tsr1-type G domain / Ribosome biogenesis protein Bms1/Tsr1 / 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal / AARP2CN (NUC121) domain / Bms1-type guanine nucleotide-binding (G) domain profile. / AARP2CN (NUC121) domain / Protein of unknown function (DUF663) / PIN domain / 40S ribosomal protein SA / K Homology domain, type 1 superfamily / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / : / Ribosomal protein S7e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S27e signature. / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S6, eukaryotic / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S19A/S15e / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein S7e / Ribosomal protein S7e / : / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e

Component:

Serine/threonine-protein kinase RIO3 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS28 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1 / Pre-rRNA-processing protein TSR1 homolog / Protein LTV1 homolog / RNA-binding protein PNO1 / RNA-binding protein NOB1

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.8 Å
• Authors: Huang Z / Wang M / Li Y / Beckmann R / Cheng J

Funding support

1 items

Organization	Grant number	Country
Not funded

• Citation: Journal: Mol Cell / Year: 2025; Title: RIOK3 mediates the degradation of 40S ribosomes.; Authors: Zixuan Huang / Frances F Diehl / Mengjiao Wang / Yi Li / Aixia Song / Fei Xavier Chen / Nicolle A Rosa-Mercado / Roland Beckmann / Rachel Green / Jingdong Cheng / ; Abstract: Cells tightly regulate ribosome homeostasis to adapt to changing environments. Ribosomes are degraded during stress, but the mechanisms responsible remain unclear. Here, we show that starvation induces the selective depletion of 40S ribosomes following their ubiquitylation by the E3 ligase RNF10. The atypical kinase RIOK3 specifically recognizes these ubiquitylated 40S ribosomes through a unique ubiquitin-interacting motif, visualized by cryoelectron microscopy (cryo-EM). RIOK3 binding and ubiquitin recognition are essential for 40S ribosome degradation during starvation. RIOK3 induces the degradation of ubiquitylated 40S ribosomes through progressive decay of their 18S rRNA beginning at the 3' end, as revealed by cryo-EM structures of degradation intermediates. Together, these data define a pathway and mechanism for stress-induced degradation of 40S ribosomes, directly connecting ubiquitylation to regulation of ribosome homeostasis.

History

Deposition	May 1, 2024	-
Header (metadata) release	Feb 12, 2025	-
Map release	Feb 12, 2025	-
Update	Mar 12, 2025	-
Current status	Mar 12, 2025	Processing site: PDBc / Status: Released

Map

• File: Download / File: emd_39957.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: local_resolution filtered map
• Voxel size: X=Y=Z: 1.064 Å

Density

Contour Level	By AUTHOR: 0.02
Minimum - Maximum	-0.16070834 - 0.35848603
Average (Standard dev.)	0.0011643698 (±0.009515517)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 383.04 Å α=β=γ: 90.0 °

Supplemental data

Additional map: multibody refinement focusing on 40S body

• File: emd_39957_additional_1.map
• Annotation: multibody refinement focusing on 40S body

Additional map: consensus map

• File: emd_39957_additional_2.map
• Annotation: consensus map

Additional map: multibody refinement focusing on 40S head, and local...

• File: emd_39957_additional_3.map
• Annotation: multibody refinement focusing on 40S head, and local resolution filtered

Additional map: multibody refinement focusing on 40S head

• File: emd_39957_additional_4.map
• Annotation: multibody refinement focusing on 40S head

Additional map: Filtered by DeepEMhancer

• File: emd_39957_additional_5.map
• Annotation: Filtered by DeepEMhancer

Additional map: multibody refinement focusing on 40S body, and local...

• File: emd_39957_additional_6.map
• Annotation: multibody refinement focusing on 40S body, and local resolution filtered

Half map: #2

• File: emd_39957_half_map_1.map

Half map: #1

• File: emd_39957_half_map_2.map

Sample components

Entire : ubiquitylated pre-40S ribosome with RIOK3

• Entire: Name: ubiquitylated pre-40S ribosome with RIOK3

Components

• Complex: ubiquitylated pre-40S ribosome with RIOK3
  • RNA: 18S rRNA
  • Protein or peptide: Small ribosomal subunit protein eS17
  • Protein or peptide: Small ribosomal subunit protein uS2
  • Protein or peptide: Small ribosomal subunit protein eS1
  • Protein or peptide: Small ribosomal subunit protein uS5
  • Protein or peptide: Small ribosomal subunit protein eS4, X isoform
  • Protein or peptide: Small ribosomal subunit protein eS6
  • Protein or peptide: Small ribosomal subunit protein eS7
  • Protein or peptide: Small ribosomal subunit protein eS8
  • Protein or peptide: Small ribosomal subunit protein uS4
  • Protein or peptide: Small ribosomal subunit protein uS17
  • Protein or peptide: Small ribosomal subunit protein uS15
  • Protein or peptide: Small ribosomal subunit protein uS11
  • Protein or peptide: Small ribosomal subunit protein eS21
  • Protein or peptide: Small ribosomal subunit protein uS8
  • Protein or peptide: Small ribosomal subunit protein uS12
  • Protein or peptide: Small ribosomal subunit protein eS24
  • Protein or peptide: Small ribosomal subunit protein eS27
  • Protein or peptide: RNA-binding protein PNO1
  • Protein or peptide: RNA-binding protein NOB1
  • Protein or peptide: Pre-rRNA-processing protein TSR1 homolog
  • Protein or peptide: Protein LTV1 homolog
  • Protein or peptide: Small ribosomal subunit protein uS14
  • Protein or peptide: Small ribosomal subunit protein uS3
  • Protein or peptide: Small ribosomal subunit protein uS7
  • Protein or peptide: Small ribosomal subunit protein eS10
  • Protein or peptide: Small ribosomal subunit protein eS12
  • Protein or peptide: Small ribosomal subunit protein uS19
  • Protein or peptide: Small ribosomal subunit protein uS9
  • Protein or peptide: Small ribosomal subunit protein uS13
  • Protein or peptide: Small ribosomal subunit protein eS19
  • Protein or peptide: Small ribosomal subunit protein uS10
  • Protein or peptide: Small ribosomal subunit protein eS25
  • Protein or peptide: Small ribosomal subunit protein eS28
  • Protein or peptide: Small ribosomal subunit protein RACK1
  • Protein or peptide: Serine/threonine-protein kinase RIO3
  • Protein or peptide: Ubiquitin
• Ligand: ZINC ION

Supramolecule #1: ubiquitylated pre-40S ribosome with RIOK3

• Supramolecule: Name: ubiquitylated pre-40S ribosome with RIOK3 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#37
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 604.10075 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC CACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGGCGC UGACCCCCUU CGCGGGGGGG AUGCGUGCAU UUAUCAGAUC AAAACCAACC CG GUCAGCC CCUCUCCGGC CCCGGCCGGG GGGCGGGCGC CGGCGGCUUU GGUGACUCUA GAUAACCUCG GGCCGAUCGC ACG CCCCCC GUGGCGGCGA CGACCCAUUC GAACGUCUGC CCUAUCAACU UUCGAUGGUA GUCGCCGUGC CUACCAUGGU GACC ACGGG UGACGGGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCG CGCA AAUUACCCAC UCCCGACCCG GGGAGGUAGU GACGAAAAAU AACAAUACAG GACUCUUUCG AGGCCCUGUA AUUGGA AUG AGUCCACUUU AAAUCCUUUA ACGAGGAUCC AUUGGAGGGC AAGUCUGGUG CCAGCAGCCG CGGUAAUUCC AGCUCCA AU AGCGUAUAUU AAAGUUGCUG CAGUUAAAAA GCUCGUAGUU GGAUCUUGGG AGCGGGCGGG CGGUCCGCCG CGAGGCGA G CCACCGCCCG UCCCCGCCCC UUGCCUCUCG GCGCCCCCUC GAUGCUCUUA GCUGAGUGUC CCGCGGGGCC CGAAGCGUU UACUUUGAAA AAAUUAGAGU GUUCAAAGCA GGCCCGAGCC GCCUGGAUAC CGCAGCUAGG AAUAAUGGAA UAGGACCGCG GUUCUAUUU UGUUGGUUUU CGGAACUGAG GCCAUGAUUA AGAGGGACGG CCGGGGGCAU UCGUAUUGCG CCGCUAGAGG U GAAAUUCU UGGACCGGCG CAAGACGGAC CAGAGCGAAA GCAUUUGCCA AGAAUGUUUU CAUUAAUCAA GAACGAAAGU CG GAGGUUC GAAGACGAUC AGAUACCGUC GUAGUUCCGA CCAUAAACGA UGCCGACCGG CGAUGCGGCG GCGUUAUUCC CAU GACCCG CCGGGCAGCU UCCGGGAAAC CAAAGUCUUU GGGUUCCGGG GGGAGUAUGG UUGCAAAGCU GAAACUUAAA GGAA UUGAC GGAAGGGCAC CACCAGGAGU GGAGCCUGCG GCUUAAUUUG ACUCAACACG GGAAACCUCA CCCGGCCCGG ACACG GACA GGAUUGACAG AUUGAUAGCU CUUUCUCGAU UCCGUGGGUG GUGGUGCAUG GCCGUUCUUA GUUGGUGGAG CGAUUU GUC UGGUUAAUCC CGAUAACGAA CGAGACUCUG GCAUGCUAAC UAGUUACGCG ACCCCCGAGC GGUCGGCGUC CCCCAAC UU CUUAGAGGGA CAAGUGGCGU UCAGCCACCC GAGAUUGAGC AAUAACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUG C ACGCGCGCUA CACUGACUGG CUCAGCGUGU GCCUACCCUA CGCCGGCAGG CGCGGGUAAC CCGUUGAACC CCAUUCGUG AUGGGGAUCG GGGAUUGCAA UUAUUCCCCA UGAACGAGGA AUUCCCAGUA AGUGCGGGUC AUAAGCUUGC GUUGAUUAAG UCCCUGCCC UUUGUACACA CCGCCCGUCG CUACUACCGA UUGGAUGGUU UAGUGAGGCC CUCGGAUCGG CCCCGCCGGG G UCGGCCCA CGGCCCUGGC GGAGCGCUGA GAAGACGGUC GAACUUGACU AUCUAGAGGA AGUAAAAGUC GUAACAAGGU UU CCGUAGG UGAACCUGCG GAAGGAUCAU UAACGG

Macromolecule #2: Small ribosomal subunit protein eS17

• Macromolecule: Name: Small ribosomal subunit protein eS17 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.578156 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGPV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #3: Small ribosomal subunit protein uS2

• Macromolecule: Name: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 32.883938 KDa

Sequence

String:

MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGATTDWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #4: Small ribosomal subunit protein eS1

• Macromolecule: Name: Small ribosomal subunit protein eS1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #5: Small ribosomal subunit protein uS5

• Macromolecule: Name: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 31.376516 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG NRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW MPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #6: Small ribosomal subunit protein eS4, X isoform

• Macromolecule: Name: Small ribosomal subunit protein eS4, X isoform / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4, X isoform

Macromolecule #7: Small ribosomal subunit protein eS6

• Macromolecule: Name: Small ribosomal subunit protein eS6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #8: Small ribosomal subunit protein eS7

• Macromolecule: Name: Small ribosomal subunit protein eS7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #9: Small ribosomal subunit protein eS8

• Macromolecule: Name: Small ribosomal subunit protein eS8 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #10: Small ribosomal subunit protein uS4

• Macromolecule: Name: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #11: Small ribosomal subunit protein uS17

• Macromolecule: Name: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #12: Small ribosomal subunit protein uS15

• Macromolecule: Name: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #13: Small ribosomal subunit protein uS11

• Macromolecule: Name: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #14: Small ribosomal subunit protein eS21

• Macromolecule: Name: Small ribosomal subunit protein eS21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.124389 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #15: Small ribosomal subunit protein uS8

• Macromolecule: Name: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #16: Small ribosomal subunit protein uS12

• Macromolecule: Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.844666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #17: Small ribosomal subunit protein eS24

• Macromolecule: Name: Small ribosomal subunit protein eS24 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.463333 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #18: Small ribosomal subunit protein eS27

• Macromolecule: Name: Small ribosomal subunit protein eS27 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #19: RNA-binding protein PNO1

• Macromolecule: Name: RNA-binding protein PNO1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.970355 KDa

Sequence

String:

MESEMETQSA RAEEGFTQVT RKGGRRAKKR QAEQLSAAGE GGDAGRMDTE EARPAKRPVF PPLCGDGLLS GKEETRKIPV PANRYTPLK ENWMKIFTPI VEHLGLQIRF NLKSRNVEIR TCKETKDVSA LTKAADFVKA FILGFQVEDA LALIRLDDLF L ESFEITDV KPLKGDHLSR AIGRIAGKGG KTKFTIENVT RTRIVLADVK VHILGSFQNI KMARTAICNL ILGNPPSKVY GN IRAVASR SADRF

UniProtKB: RNA-binding protein PNO1

Macromolecule #20: RNA-binding protein NOB1

• Macromolecule: Name: RNA-binding protein NOB1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO / EC number: Hydrolases; Acting on ester bonds
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.743914 KDa

Sequence

String:

MAPVEHVVAD AGAFLRHAAL QDIGKNIYTI REVVTEIRDK ATRRRLAVLP YELRFKEPLP EYVRLVTEFS KKTGDYPSLS ATDIQVLAL TYQLEAEFVG VSHLKQEPQK VKVSSSIQHP ETPLHISGFH LPYKPKPPQE TEKGHSACEP ENLEFSSFMF W RNPLPNID HELQELLIDR GEDVPSEEEE EEENGFEDRK DDSDDDGGGW ITPSNIKQIQ QELEQCDVPE DVRVGCLTTD FA MQNVLLQ MGLHVLAVNG MLIREARSYI LRCHGCFKTT SDMSRVFCSH CGNKTLKKVS VTVSDDGTLH MHFSRNPKVL NPR GLRYSL PTPKGGKYAI NPHLTEDQRF PQLRLSQKAR QKTNVFAPDY IAGVSPFVEN DISSRSATLQ VRDSTLGAGR RRLN PNASR KKFVKKR

UniProtKB: RNA-binding protein NOB1

Macromolecule #21: Pre-rRNA-processing protein TSR1 homolog

• Macromolecule: Name: Pre-rRNA-processing protein TSR1 homolog / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 91.951188 KDa

Sequence

String:

MAAHRPGPLK QQNKAHKGGR HRGRGSAQRD GKGRLALKTL SKKVRKELSR VDQRHRASQL RKQKKEAVLA EKRQLGGKDG PPHQVLVVP LHSRISLPEA MQLLQDRDTG TVHLNELGNT QNFMLLCPRL KHRWFFTSAR PGDLHVVLDM AKVADTILFL L DPLEGWDS TGDYCLSCLF AQGLPTYTLA VQGISGLPLK KQIDTRKKLS KAVEKRFPHD KLLLLDTQQE AGMLLRQLAN QK QQHLAFR DRRAYLFAHA VDFVPSEENN LVGTLKISGY VRGQTLNVNR LLHIVGYGDF QMKQIDAPGD PFPLNPRGIK PQK DPDMAM EICATDAVDD MEEGLKVLMK ADPGRQESLQ AEVIPDPMEG EQTWPTEEEL SEAKDFLKES SKVVKKVPKG TSSY QAEWI LDGGSQSGGE GDEYEYDDME HEDFMEEESQ DESSEEEEEY ETMTIGESVH DDLYDKKVDE EAEAKMLEKY KQERL EEMF PDEVDTPRDV AARIRFQKYR GLKSFRTSPW DPKENLPQDY ARIFQFQNFT NTRKSIFKEV EEKEVEGAEV GWYVTL HVS EVPVSVVECF RQGTPLIAFS LLPHEQKMSV LNMVVRRDPG NTEPVKAKEE LIFHCGFRRF RASPLFSQHT AADKHKL QR FLTADMALVA TVYAPITFPP ASVLLFKQKS NGMHSLIATG HLMSVDPDRM VIKRVVLSGH PFKIFTKMAV VRYMFFNR E DVLWFKPVEL RTKWGRRGHI KEPLGTHGHM KCSFDGKLKS QDTVLMNLYK RVFPKWTYDP YVPEPVPWLK SEISSTVPQ GGME

UniProtKB: Pre-rRNA-processing protein TSR1 homolog

Macromolecule #22: Protein LTV1 homolog

• Macromolecule: Name: Protein LTV1 homolog / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 54.935891 KDa

Sequence

String:

MPHRKKKPFI EKKKAVSFHL VHRSQRDPLA ADESAPQRVL LPTQKIDNEE RRAEQRKYGV FFDDDYDYLQ HLKEPSGPSE LIPSSTFSA HNRREEKEET LVIPSTGIKL PSSVFASEFE EDVGLLNKAA PVSGPRLDFD PDIVAALDDD FDFDDPDNLL E DDFILQAN KATGEEEGMD IQKSENEDDS EWEDVDDEKG DSNDDYDSAG LLSDEDCMSV PGKTHRAIAD HLFWSEETKS RF TEYSMTS SVMRRNEQLT LHDERFEKFY EQYDDDEIGA LDNAELEGSI QVDSNRLQEV LNDYYKEKAE NCVKLNTLEP LED QDLPMN ELDESEEEEM ITVVLEEAKE KWDCESICST YSNLYNHPQL IKYQPKPKQI RISSKTGIPL NVLPKKGLTA KQTE RIQMI NGSDLPKVST QPRSKNESKE DKRARKQAIK EERKERRVEK KANKLAFKLE KRRQEKELLN LKKNVEGLKL

UniProtKB: Protein LTV1 homolog

Macromolecule #23: Small ribosomal subunit protein uS14

• Macromolecule: Name: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #24: Small ribosomal subunit protein uS3

• Macromolecule: Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.729369 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPTGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #25: Small ribosomal subunit protein uS7

• Macromolecule: Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #26: Small ribosomal subunit protein eS10

• Macromolecule: Name: Small ribosomal subunit protein eS10 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #27: Small ribosomal subunit protein eS12

• Macromolecule: Name: Small ribosomal subunit protein eS12 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.538987 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #28: Small ribosomal subunit protein uS19

• Macromolecule: Name: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.076207 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #29: Small ribosomal subunit protein uS9

• Macromolecule: Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.477377 KDa

Sequence

String:

MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #30: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.759777 KDa

Sequence

String:

MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #31: Small ribosomal subunit protein eS19

• Macromolecule: Name: Small ribosomal subunit protein eS19 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.091562 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #32: Small ribosomal subunit protein uS10

• Macromolecule: Name: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #33: Small ribosomal subunit protein eS25

• Macromolecule: Name: Small ribosomal subunit protein eS25 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.776224 KDa

Sequence

String:

MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #34: Small ribosomal subunit protein eS28

• Macromolecule: Name: Small ribosomal subunit protein eS28 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #35: Small ribosomal subunit protein RACK1

• Macromolecule: Name: Small ribosomal subunit protein RACK1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #36: Serine/threonine-protein kinase RIO3

• Macromolecule: Name: Serine/threonine-protein kinase RIO3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO / EC number: non-specific serine/threonine protein kinase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 59.178945 KDa

Sequence

String:

MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA VAEGPFITGE NIDTSSDLML AQMLQMEYD REYDAQLRRE EKKFNGDSKV SISFENYRKV HPYEDSDSSE DEVDWQDTRD DPYRPAKPVP TPKKGFIGKG K DITTKHDE VVCGRKNTAR MENFAPEFQV GDGIGMDLKL SNHVFNALKQ HAYSEERRSA RLHEKKEHST AEKAVDPKTR LL MYKMVNS GMLETITGCI STGKESVVFH AYGGSMEDEK EDSKVIPTEC AIKVFKTTLN EFKNRDKYIK DDFRFKDRFS KLN PRKIIR MWAEKEMHNL ARMQRAGIPC PTVVLLKKHI LVMSFIGHDQ VPAPKLKEVK LNSEEMKEAY YQTLHLMRQL YHEC TLVHA DLSEYNMLWH AGKVWLIDVS QSVEPTHPHG LEFLFRDCRN VSQFFQKGGV KEALSERELF NAVSGLNITA DNEAD FLAE IEALEKMNED HVQKNGRKAA SFLKDDGDPP LLYDE

UniProtKB: Serine/threonine-protein kinase RIO3

Macromolecule #37: Ubiquitin

• Macromolecule: Name: Ubiquitin / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.576831 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #38: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 38 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 58.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

4351

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 29221
• Initial angle assignment: Type: OTHER / Details: Relion
• Final angle assignment: Type: OTHER / Details: Relion