EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	RIOK3 mediates the degradation of 40S ribosomes.
ジャーナル・号・ページ	Mol Cell, Vol. 85, Issue 4, Page 802-814.e12, Year 2025
掲載日	2025年2月20日
著者	Zixuan Huang / Frances F Diehl / Mengjiao Wang / Yi Li / Aixia Song / Fei Xavier Chen / Nicolle A Rosa-Mercado / Roland Beckmann / Rachel Green / Jingdong Cheng /
PubMed 要旨	Cells tightly regulate ribosome homeostasis to adapt to changing environments. Ribosomes are degraded during stress, but the mechanisms responsible remain unclear. Here, we show that starvation ...Cells tightly regulate ribosome homeostasis to adapt to changing environments. Ribosomes are degraded during stress, but the mechanisms responsible remain unclear. Here, we show that starvation induces the selective depletion of 40S ribosomes following their ubiquitylation by the E3 ligase RNF10. The atypical kinase RIOK3 specifically recognizes these ubiquitylated 40S ribosomes through a unique ubiquitin-interacting motif, visualized by cryoelectron microscopy (cryo-EM). RIOK3 binding and ubiquitin recognition are essential for 40S ribosome degradation during starvation. RIOK3 induces the degradation of ubiquitylated 40S ribosomes through progressive decay of their 18S rRNA beginning at the 3' end, as revealed by cryo-EM structures of degradation intermediates. Together, these data define a pathway and mechanism for stress-induced degradation of 40S ribosomes, directly connecting ubiquitylation to regulation of ribosome homeostasis.
リンク	Mol Cell / PubMed:39947183
手法	EM (単粒子)
解像度	3.6 - 8.2 Å
構造データ	39956 8zdb EMDB-39956, PDB-8zdb: Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 手法: EM (単粒子) / 解像度: 3.6 Å 39957 8zdc EMDB-39957, PDB-8zdc: Cryo-EM structure of the human ubiquitylated pre-40S ribosome with RIOK3 手法: EM (単粒子) / 解像度: 3.8 Å 39958 8zdd EMDB-39958, PDB-8zdd: Cryo-EM structure of the human ubiquitylated pre-40S ribosome with RIOK3 (without NOB1) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-39959: Cryo-EM structure of the human ubiquitylated 40S ribosome dimer with RIOK3 手法: EM (単粒子) / 解像度: 8.2 Å EMDB-39960: Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg1) 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-39962: Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg2) 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-39963: Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg3) 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-39964: Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg4) 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	RIBOSOME / ubiquitylation / RIOK3