登録情報 データベース : EMDB / ID : EMD-38799 ダウンロードとリンクタイトル Architecture of the spinach plastid-encoded RNA polymerase マップデータ 詳細 試料複合体 : spinach plastid-encoded RNA polymeraseリガンド : x 1種 詳細 キーワード plastid-encoded RNA polymerase / chloroplast / spinach / TRANSCRIPTION機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
etioplast organization / plastid transcription / positive regulation of red or far-red light signaling pathway / plastid-encoded plastid RNA polymerase complex / plastid organization / thylakoid membrane organization / chloroplast nucleoid / acid-amino acid ligase activity / chloroplast organization / chloroplast thylakoid ... etioplast organization / plastid transcription / positive regulation of red or far-red light signaling pathway / plastid-encoded plastid RNA polymerase complex / plastid organization / thylakoid membrane organization / chloroplast nucleoid / acid-amino acid ligase activity / chloroplast organization / chloroplast thylakoid / thylakoid / protein-lysine N-methyltransferase activity / biosynthetic process / superoxide dismutase / superoxide dismutase activity / protein-disulfide reductase activity / response to light stimulus / : / DNA-directed RNA polymerase complex / cell redox homeostasis / chloroplast / ribonucleoside binding / : / : / : / : / : / : / DNA-directed RNA polymerase / kinase activity / regulation of cell shape / methylation / protein dimerization activity / cell division / positive regulation of DNA-templated transcription / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / ATP binding / metal ion binding / nucleus / cytoplasm 類似検索 - 分子機能 Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12 / Protein plastid transcriptionally active 7 / PLASTID TRANSCRIPTIONALLY ACTIVE protein 6 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 / Thioredoxin-like protein CITRX, chloroplastic / (S)-ureidoglycine aminohydrolase, cupin domain / EutQ-like cupin domain / : / DNA-directed RNA polymerase subunit RpoC1 / DNA-directed RNA polymerase, subunit beta'' ... Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12 / Protein plastid transcriptionally active 7 / PLASTID TRANSCRIPTIONALLY ACTIVE protein 6 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 / Thioredoxin-like protein CITRX, chloroplastic / (S)-ureidoglycine aminohydrolase, cupin domain / EutQ-like cupin domain / : / DNA-directed RNA polymerase subunit RpoC1 / DNA-directed RNA polymerase, subunit beta'' / UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase / Rubisco LSMT, substrate-binding domain / Rubisco LSMT, substrate-binding domain superfamily / : / Rubisco LSMT substrate-binding / MurE/MurF, N-terminal / : / SAP domain superfamily / Mur ligase, C-terminal / Mur ligase, C-terminal domain superfamily / Mur ligase, glutamate ligase domain / Mur ligase, central / Mur-like, catalytic domain superfamily / Mur ligase middle domain / Manganese/iron superoxide dismutase, binding site / Manganese and iron superoxide dismutases signature. / Manganese/iron superoxide dismutase / Manganese/iron superoxide dismutase, N-terminal / Iron/manganese superoxide dismutases, alpha-hairpin domain / SAP motif profile. / SAP domain / Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation / SAP domain / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Carbohydrate kinase PfkB / pfkB family carbohydrate kinase / Ribokinase-like / Thioredoxin / SET domain / SET domain superfamily / SET domain profile. / SET domain / S1 domain profile. / RmlC-like cupin domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase beta subunit, bacterial-type / Thioredoxin domain profile. / S1 domain / Thioredoxin domain / RmlC-like jelly roll fold / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 3 superfamily / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, RBP11-like subunit / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / Tetratricopeptide-like helical domain superfamily / Thioredoxin-like superfamily / Nucleic acid-binding, OB-fold 類似検索 - ドメイン・相同性 Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7 / Uncharacterized protein LOC110792506 isoform X1 / superoxide dismutase / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X2 / Uncharacterized protein LOC110798943 / Thioredoxin-like protein CITRX, chloroplastic / Fructokinase-like 1, chloroplastic / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 / Uncharacterized protein LOC110782000 / superoxide dismutase ... Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7 / Uncharacterized protein LOC110792506 isoform X1 / superoxide dismutase / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X2 / Uncharacterized protein LOC110798943 / Thioredoxin-like protein CITRX, chloroplastic / Fructokinase-like 1, chloroplastic / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 / Uncharacterized protein LOC110782000 / superoxide dismutase / Uncharacterized protein LOC110791626 / Fructokinase-like 2, chloroplastic isoform X2 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12 / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase subunit beta'' / DNA-directed RNA polymerase subunit beta' 類似検索 - 構成要素生物種 Spinacia oleracea (ホウレンソウ)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.16 Å 詳細 データ登録者Wang G-L / Yu L-J / Lu C 資金援助 中国, 3件 詳細 詳細を隠すOrganization Grant number 国 Ministry of Science and Technology (MoST, China) 2022YFC3401800 中国 Ministry of Science and Technology (MoST, China) 2020YFA0907600 中国 National Natural Science Foundation of China (NSFC) 32000444 中国
引用ジャーナル : Nat Commun / 年 : 2024タイトル : Architecture of the spinach plastid-encoded RNA polymerase.著者 : Tongtong Wang / Guang-Lei Wang / Ying Fang / Yi Zhang / Wenxin Peng / Yue Zhou / Aihong Zhang / Long-Jiang Yu / Congming Lu / 要旨 : The plastid-encoded RNA polymerase serves as the principal transcription machinery within chloroplasts, transcribing over 80% of all primary plastid transcripts. This polymerase consists of a ... The plastid-encoded RNA polymerase serves as the principal transcription machinery within chloroplasts, transcribing over 80% of all primary plastid transcripts. This polymerase consists of a prokaryotic-like core enzyme known as the plastid-encoded RNA polymerase core, and is supplemented by newly evolved associated proteins known as PAPs. However, the architecture of the plastid-encoded RNA polymerase and the possible functions of PAPs remain unknown. Here, we present the cryo-electron microscopy structure of a 19-subunit plastid-encoded RNA polymerase complex derived from spinach (Spinacia oleracea). The structure shows that the plastid-encoded RNA polymerase core resembles bacterial RNA polymerase. Twelve PAPs and two additional proteins (FLN2 and pTAC18) bind at the periphery of the plastid-encoded RNA polymerase core, forming extensive interactions that may facilitate complex assembly and stability. PAPs may also protect the complex against oxidative damage and has potential functions in transcriptional regulation. This research offers a structural basis for future investigations into the functions and regulatory mechanisms governing the transcription of plastid genes. 履歴 登録 2024年1月21日 - ヘッダ(付随情報) 公開 2024年12月4日 - マップ公開 2024年12月4日 - 更新 2024年12月4日 - 現状 2024年12月4日 処理サイト : PDBc / 状態 : 公開
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