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-Structure paper
| タイトル | Architecture of the spinach plastid-encoded RNA polymerase. |
|---|---|
| ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 9838, Year 2024 |
| 掲載日 | 2024年11月13日 |
著者 | Tongtong Wang / Guang-Lei Wang / Ying Fang / Yi Zhang / Wenxin Peng / Yue Zhou / Aihong Zhang / Long-Jiang Yu / Congming Lu / ![]() |
| PubMed 要旨 | The plastid-encoded RNA polymerase serves as the principal transcription machinery within chloroplasts, transcribing over 80% of all primary plastid transcripts. This polymerase consists of a ...The plastid-encoded RNA polymerase serves as the principal transcription machinery within chloroplasts, transcribing over 80% of all primary plastid transcripts. This polymerase consists of a prokaryotic-like core enzyme known as the plastid-encoded RNA polymerase core, and is supplemented by newly evolved associated proteins known as PAPs. However, the architecture of the plastid-encoded RNA polymerase and the possible functions of PAPs remain unknown. Here, we present the cryo-electron microscopy structure of a 19-subunit plastid-encoded RNA polymerase complex derived from spinach (Spinacia oleracea). The structure shows that the plastid-encoded RNA polymerase core resembles bacterial RNA polymerase. Twelve PAPs and two additional proteins (FLN2 and pTAC18) bind at the periphery of the plastid-encoded RNA polymerase core, forming extensive interactions that may facilitate complex assembly and stability. PAPs may also protect the complex against oxidative damage and has potential functions in transcriptional regulation. This research offers a structural basis for future investigations into the functions and regulatory mechanisms governing the transcription of plastid genes. |
リンク | Nat Commun / PubMed:39537621 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.16 - 3.87 Å |
| 構造データ | EMDB-38799, PDB-8xzv: ![]() EMDB-61149: RpoC2 SI domain of the spinach plastid-encoded RNA polymerase ![]() EMDB-61151: MurE subunit of the spinach plastid-encoded RNA polymerase |
| 化合物 | ![]() ChemComp-FE: |
| 由来 |
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キーワード | TRANSCRIPTION / plastid-encoded RNA polymerase / chloroplast / spinach |
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spinacia oleracea (ホウレンソウ)
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