|Entry||Database: EMDB / ID: 3878|
|Title||The distal end of a non-contractile T6SS sheath.|
|Sample||The distal end of a non-contractile T6SS sheath.|
|Source||Vibrio cholerae / bacteria /|
|Map data||The distal end of a non-contractile T6SS sheath.|
|Method||single particle reconstruction, at 7.5 Å resolution|
|Authors||Nazarov S / Basler M|
|Citation||EMBO J., 2018, 37|
|Date||Deposition: Sep 18, 2017 / Header (metadata) release: Nov 29, 2017 / Map release: Nov 29, 2017 / Last update: Feb 28, 2018|
Downloads & links
|File||emd_3878.map.gz (map file in CCP4 format, 67109 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 2.12 Å|
CCP4 map header:
|File||emd_3878_msk_1.map ( map file in CCP4 format, 67109 KB )|
|Projections & Slices|
|Data type||Image stored as Reals|
|Annotation details||Mask for focused 3D refinement and post-processing.|
|Space group number||1|
-Entire The distal end of a non-contractile T6SS sheath.
|Entire||Name: The distal end of a non-contractile T6SS sheath. / Number of components: 1|
|Mass||Experimental: 540 kDa|
-Component #1: cellular-component, The distal end of a non-contractile T6SS sheath.
|Cellular-component||Name: The distal end of a non-contractile T6SS sheath. / Recombinant expression: No|
|Mass||Experimental: 540 kDa|
|Source||Species: Vibrio cholerae / bacteria / / Strain: 2740-80|
|Source (natural)||Organelle: T6SS|
|Sample solution||pH: 8.3|
|Vitrification||Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277 K / Details: blot for 3 seconds before plunging|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 5 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 130000 X (nominal) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 1500 - 3000 nm / Energy filter: Gatan Quantum-LS Energy Filter|
|Specimen Holder||Model: FEI TITAN KRIOS AUTOGRID HOLDER|
|Camera||Detector: GATAN K2 (4k x 4k)|
|Image acquisition||Number of digital images: 9202|
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Three pioneers of this field were awarded Nobel Prize in Chemistry 2017
- Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
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External links: The 2017 Nobel Prize in Chemistry - Press Release
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