PDB-6vu3: Cryo-EM structure of Escherichia coli transcription-translation c...

ID or keywords:

Entry

Database: PDB / ID: 6vu3

Title

Cryo-EM structure of Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer

Components

(30S ribosomal protein ...) x 21
(50S ribosomal protein ...) x 32
(DNA-directed RNA polymerase subunit ...Polymerase) x 3
16S rRNA
23S rRNA23S ribosomal RNA
5S rRNA5S ribosomal RNA
E-site and A-site tRNA (fMet)
NT DNA
T DNA
Transcription termination/antitermination protein NusG
mRNA with 12 nt long spacer

Keywords

RIBOSOME / TRANSCRIPTION/TRANSLATION / bacterial coupled transcription-translation complex / TRANSCRIPTION-TRANSLATION complex

Function / homology

Function and homology information

transcription elongation-coupled chromatin remodeling / DnaA-L2 complex / negative regulation of DNA-templated DNA replication initiation /

DNA-directed RNA polymerase complex / regulation of DNA-templated transcription elongation / transcription antitermination / DNA-templated transcription termination /

ribonucleoside binding / small ribosomal subunit rRNA binding / DNA-directed 5'-3' RNA polymerase activity ...
Similarity search - Function

Ribosomal protein S1 /

Ribosomal protein S1-like /

: / Transcription antitermination protein, NusG / Transcription antitermination protein, NusG, bacteria, conserved site / Transcription termination factor nusG signature. / NusG-like / Transcription termination factor nusG /

Ribosomal protein L7/L12, oligomerisation /

Ribosomal protein L7/L12, oligomerisation domain superfamily ...

Ribosomal protein S1 /

Ribosomal protein S1-like /

: / Transcription antitermination protein, NusG / Transcription antitermination protein, NusG, bacteria, conserved site / Transcription termination factor nusG signature. / NusG-like / Transcription termination factor nusG /

Ribosomal protein L7/L12, oligomerisation /

Ribosomal protein L7/L12, oligomerisation domain superfamily /

Ribosomal protein L7/L12 dimerisation domain /

Ribosomal protein L7/L12 /

Ribosomal protein L7/L12, C-terminal /

Ribosomal protein L7/L12 C-terminal domain /

RNA-binding domain, S1 /

Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / NusG, N-terminal domain superfamily /

Ribosomal protein L10, eubacterial, conserved site /

Ribosomal protein L10 signature. /

Ribosomal protein L10 / S1 domain profile. /

DNA-directed RNA polymerase, subunit beta-prime, bacterial type /

Ribosomal protein S21, conserved site /

Ribosomal protein S21 signature. /

DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily /

DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain /

RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain /

DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase beta subunit, bacterial-type /

Ribosomal protein L25, short-form /

Ribosomal protein S14, bacterial/plastid /

Ribosomal protein S1-like RNA-binding domain /

Ribosomal protein L11, bacterial-type / S1 RNA binding domain /

Ribosomal protein L31 type A /

Ribosomal protein S21 superfamily /

S1 domain /

Ribosomal protein S21 /

Ribosomal protein S16, conserved site /

Ribosomal protein S16 signature. /

Ribosomal protein L31 signature. /

Ribosomal protein S21 /

Ribosomal protein L31 /

Ribosomal protein L31 superfamily /

Ribosomal protein L31 /

Ribosomal protein L11, conserved site /

Ribosomal protein L10-like domain superfamily /

Ribosomal protein L21, conserved site /

Ribosomal protein L21 signature. /

Ribosomal protein L10P /

Ribosomal protein L10 /

Ribosomal protein L11 signature. /

Ribosomal protein L16 signature 1. /

: /

Ribosomal protein L6, conserved site /

Ribosomal protein L6 signature 1. /

Ribosomal protein L16, conserved site /

Ribosomal protein L16 signature 2. /

Ribosomal protein L11, N-terminal /

Ribosomal protein L17 signature. /

Ribosomal protein L9 signature. /

Ribosomal protein L9, bacteria/chloroplast /

Ribosomal protein L9, C-terminal /

Ribosomal protein L9, C-terminal domain /

Ribosomal protein L9, C-terminal domain superfamily /

Ribosomal protein L11/L12 /

Ribosomal protein L11, C-terminal /

Ribosomal protein L11, C-terminal domain superfamily /

Ribosomal protein L11/L12, N-terminal domain superfamily /

Ribosomal protein L11/L12 /

Ribosomal protein L11, N-terminal domain /

Ribosomal protein L11, RNA binding domain / Ribosomal L25p family /

Ribosomal protein L25 /

Ribosomal protein L28/L24 superfamily /

Ribosomal protein L36 signature. /

Ribosomal protein L25/Gln-tRNA synthetase, N-terminal /

Ribosomal protein L32p, bacterial type /

Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily /

Ribosomal protein L9, N-terminal domain superfamily /

Ribosomal protein L9 /

Ribosomal protein L9, N-terminal /

Ribosomal protein L9, N-terminal domain /

Ribosomal protein L28 /

Ribosomal protein L35, conserved site /

Ribosomal protein L35 signature. /

Ribosomal protein L33, conserved site /

Ribosomal protein L33 signature. /

Ribosomal protein L35, non-mitochondrial /

Ribosomal protein L5, bacterial-type /

Ribosomal protein L6, bacterial-type /

Ribosomal protein L18, bacterial-type /

Ribosomal protein L19, conserved site /

Ribosomal protein L19 signature. /

Ribosomal protein L36 /

Ribosomal protein L36 superfamily
Similarity search - Domain/homology

: /

DNA / DNA (> 10) /

RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) ...

: /

DNA / DNA (> 10) /

RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL15 /

30S ribosomal protein S10 /

50S ribosomal protein L29 /

50S ribosomal protein L27 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein bL36 /

DNA-directed RNA polymerase subunit beta /

30S ribosomal protein S18 / Large ribosomal subunit protein bL17 /

50S ribosomal protein L7/L12 /

30S ribosomal protein S3 / Small ribosomal subunit protein bS6 /

50S ribosomal protein L10 /

30S ribosomal protein S13 / Large ribosomal subunit protein bL19 /

DNA-directed RNA polymerase subunit beta' /

50S ribosomal protein L6 /

DNA-directed RNA polymerase subunit alpha /

30S ribosomal protein S1 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS2 /

50S ribosomal protein L23 /

30S ribosomal protein S20 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS15 /

50S ribosomal protein L4 /

50S ribosomal protein L22 /

50S ribosomal protein L14 /

50S ribosomal protein L21 /

50S ribosomal protein L13 /

50S ribosomal protein L9 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 /

50S ribosomal protein L35 / Small ribosomal subunit protein uS12 /

30S ribosomal protein S14 /

50S ribosomal protein L30 /

50S ribosomal protein L28 /

50S ribosomal protein L20 /

30S ribosomal protein S4 /

50S ribosomal protein L33 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 /

30S ribosomal protein S7 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL5 /

30S ribosomal protein S9 / Transcription termination/antitermination protein NusG / Large ribosomal subunit protein bL31
Similarity search - Component

Biological species

Escherichia coli (E. coli)
synthetic construct (others)

Method

ELECTRON MICROSCOPY /

single particle reconstruction /

cryo EM / Resolution: 3.7 Å

Authors

Molodtsov, V. / Wang, C. / Su, M. / Ebright, R.

Citation

Journal: Science / Year: 2020
Title: Structural basis of transcription-translation coupling.
Authors: Chengyuan Wang / Vadim Molodtsov / Emre Firlar / Jason T Kaelber / Gregor Blaha / Min Su / Richard H Ebright /

Abstract: In bacteria, transcription and translation are coupled processes in which the movement of RNA polymerase (RNAP)-synthesizing messenger RNA (mRNA) is coordinated with the movement of the first ...

History

Deposition	Feb 14, 2020	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Sep 2, 2020	Provider: repository / Type: Initial release
Revision 1.1	Sep 16, 2020	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Sep 23, 2020	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

Movie	Deposited structure unit Imaged by Jmol Download Superimposition on EM map EMDB-21386 Imaged by UCSF Chimera Download Movie viewer
Structure viewer	Molecule: MolmilJmol/JSmol

-
Download

PDBx/mmCIF format	6vu3.cif.gz	6.7 MB	Display	PDBx/mmCIF format
PDB format	pdb6vu3.ent.gz		Display	PDB format
PDBx/mmJSON format	6vu3.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

-
Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/vu/6vu3 ftp://data.pdbj.org/pub/pdb/validation_reports/vu/6vu3	HTTPS FTP

-
Related structure data

Related structure data	21386MC 21468C 21469C 21470C 21471C 21472C 21474C 21475C 21476C 21477C 21482C 21483C 21485C 21486C 21494C 22082C 22084C 22087C 22107C 22141C 22142C 22181C 22192C 22193C 6vyqC 6vyrC 6vysC 6vytC 6vyuC 6vywC 6vyxC 6vyyC 6vyzC 6vz2C 6vz3C 6vz5C 6vz7C 6vzjC 6x6tC 6x7fC 6x7kC 6x9qC 6xdqC 6xdrC 6xgfC 6xiiC 6xijC M: map data used to model this data C: citing same article (ref.)
Similar structure data	6vyr-d 6vyq-d 6vzj-d 6vys-d 6xij-d 6ztm-d 6ztl-d 6zu1-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

-
Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#253 - Jan 2021 Expressome similarity (134) #157 - Jan 2013 Transfer-Messenger RNA similarity (38) #121 - Jan 2010 70S Ribosomes similarity (22) #181 - Jan 2015 Cascade and CRISPR similarity (2) #130 - Oct 2010 Riboswitches similarity (2) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (44) #67 - Jul 2005 TATA-Binding Protein similarity (1) #260 - Aug 2021 Ribonuclease P similarity (2) #255 - Mar 2021 Cisplatin and DNA similarity (5) #87 - Mar 2007 Zinc Fingers similarity (2) #91 - Jul 2007 Thymine Dimers similarity (1) #229 - Jan 2019 Fluorescent RNA Aptamers similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (3) #10 - Oct 2000 Ribosome similarity (4) #227 - Nov 2018 Telomerase similarity (3) #15 - Mar 2001 Transfer RNA similarity (1) #249 - Sep 2020 SARS-CoV-2 RNA-dependent RNA Polymerase similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (11) #84 - Dec 2006 Transposase similarity (1) #135 - Mar 2011 Integrase similarity (1) #210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (3) #245 - May 2020 Spliceosomes similarity (2) #65 - May 2005 Self-splicing RNA similarity (2) #105 - Sep 2008 Ribonuclease A similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #98 - Feb 2008 Small Interfering RNA similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #266 - Feb 2022 Oligosaccharyltransferase similarity (7) #161 - May 2013 Ricin similarity (6) #143 - Nov 2011 Toll-like Receptors similarity (1) #89 - May 2007 Aconitase and Iron Regulatory Protein 1 similarity (1) #81 - Sep 2006 Elongation Factors similarity (1) #230 - Feb 2019 Initiation Factor eIF4E similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #125 - May 2010 Parvoviruses similarity (1) #131 - Nov 2010 Inteins similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #106 - Oct 2008 Poly(A) Polymerase similarity (1) #23 - Nov 2001 DNA similarity (1) #104 - Aug 2008 Selenocysteine Synthase similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (6) #86 - Feb 2007 Exosomes similarity (2)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#253 - Jan 2021
Expressome
similarity (134)
#157 - Jan 2013
Transfer-Messenger RNA
similarity (38)
#121 - Jan 2010
70S Ribosomes
similarity (22)
#181 - Jan 2015
Cascade and CRISPR
similarity (2)
#130 - Oct 2010
Riboswitches
similarity (2)
#16 - Apr 2001
Aminoacyl-tRNA Synthetases
similarity (1)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (44)
#67 - Jul 2005
TATA-Binding Protein
similarity (1)
#260 - Aug 2021
Ribonuclease P
similarity (2)
#255 - Mar 2021
Cisplatin and DNA
similarity (5)
#87 - Mar 2007
Zinc Fingers
similarity (2)
#91 - Jul 2007
Thymine Dimers
similarity (1)
#229 - Jan 2019
Fluorescent RNA Aptamers
similarity (1)
#168 - Dec 2013
DNA Helicase
similarity (1)
#146 - Feb 2012
Aminoglycoside Antibiotics
similarity (3)
#10 - Oct 2000
Ribosome
similarity (4)
#227 - Nov 2018
Telomerase
similarity (3)
#15 - Mar 2001
Transfer RNA
similarity (1)
#249 - Sep 2020
SARS-CoV-2 RNA-dependent RNA Polymerase
similarity (1)
#122 - Feb 2010
Enhanceosome
similarity (1)
#172 - Apr 2014
RecA and Rad51
similarity (1)
#48 - Dec 2003
Catabolite Activator Protein
similarity (11)
#84 - Dec 2006
Transposase
similarity (1)
#135 - Mar 2011
Integrase
similarity (1)
#210 - Jun 2017
Adenine Riboswitch in Action
similarity (1)
#180 - Dec 2014
TAL Effectors
similarity (1)
#150 - Jun 2012
Sliding Clamps
similarity (1)
#112 - Apr 2009
Oct and Sox Transcription Factors
similarity (1)
#226 - Oct 2018
Aminoglycoside Antibiotics and Resistance
similarity (3)
#245 - May 2020
Spliceosomes
similarity (2)
#65 - May 2005
Self-splicing RNA
similarity (2)
#105 - Sep 2008
Ribonuclease A
similarity (1)
#73 - Jan 2006
Topoisomerase
similarity (1)
#98 - Feb 2008
Small Interfering RNA
similarity (1)
#40 - Apr 2003
RNA Polymerase
similarity (1)
#3 - Mar 2000
DNA Polymerase
similarity (1)
#139 - Jul 2011
DNA Methyltransferase
similarity (1)
#155 - Nov 2012
Vitamin D Receptor
similarity (1)
#266 - Feb 2022
Oligosaccharyltransferase
similarity (7)
#161 - May 2013
Ricin
similarity (6)
#143 - Nov 2011
Toll-like Receptors
similarity (1)
#89 - May 2007
Aconitase and Iron Regulatory Protein 1
similarity (1)
#81 - Sep 2006
Elongation Factors
similarity (1)
#230 - Feb 2019
Initiation Factor eIF4E
similarity (1)
#119 - Nov 2009
Designed DNA Crystal
similarity (1)
#125 - May 2010
Parvoviruses
similarity (1)
#131 - Nov 2010
Inteins
similarity (1)
#160 - Apr 2013
Actinomycin
similarity (1)
#190 - Oct 2015
Two-component Systems
similarity (1)
#45 - Sep 2003
Estrogen Receptor
similarity (1)
#106 - Oct 2008
Poly(A) Polymerase
similarity (1)
#23 - Nov 2001
DNA
similarity (1)
#104 - Aug 2008
Selenocysteine Synthase
similarity (1)
#240 - Dec 2019
Hypoxia-Inducible Factors
similarity (1)
#92 - Aug 2007
Anabolic Steroids
similarity (1)
#257 - May 2021
Fetal Hemoglobin
similarity (1)
#258 - Jun 2021
Glucocorticoid Receptor and Dexamethasone
similarity (1)
#8 - Aug 2000
Restriction Enzymes
similarity (1)
#31 - Jul 2002
p53 Tumor Suppressor
similarity (1)
#7 - Jul 2000
Nucleosome
similarity (1)
#39 - Mar 2003
lac Repressor
similarity (1)
#55 - Jul 2004
DNA Ligase
similarity (1)
#33 - Sep 2002
HIV Reverse Transcriptase
similarity (1)
#241 - Jan 2020
Twenty Years of Molecules
similarity (6)
#86 - Feb 2007
Exosomes
similarity (2)

Deposited unit

0: 50S ribosomal protein L21

1: 50S ribosomal protein L22

2: 50S ribosomal protein L23

3: 50S ribosomal protein L24

4: 50S ribosomal protein L25

5: NT DNA

6: T DNA

7: mRNA with 12 nt long spacer

9: 50S ribosomal protein L10

A: E-site and A-site tRNA (fMet)

AA: DNA-directed RNA polymerase subunit beta

AB: Transcription termination/antitermination protein NusG

AC: DNA-directed RNA polymerase subunit alpha

AD: DNA-directed RNA polymerase subunit alpha

AE: DNA-directed RNA polymerase subunit beta'

B: E-site and A-site tRNA (fMet)

C: 30S ribosomal protein S18

D: 16S rRNA

E: 30S ribosomal protein S20

F: 30S ribosomal protein S21

G: 30S ribosomal protein S2

H: 30S ribosomal protein S1

I: 30S ribosomal protein S3

J: 30S ribosomal protein S4

K: 30S ribosomal protein S5

L: 30S ribosomal protein S6

M: 30S ribosomal protein S7

N: 30S ribosomal protein S8

O: 30S ribosomal protein S9

P: 30S ribosomal protein S10

Q: 30S ribosomal protein S11

R: 30S ribosomal protein S12

S: 30S ribosomal protein S14

T: 30S ribosomal protein S15

U: 30S ribosomal protein S16

V: 30S ribosomal protein S17

W: 30S ribosomal protein S19

X: 30S ribosomal protein S13

Y: 50S ribosomal protein L11

Z: 50S ribosomal protein L7/L12

a: 23S rRNA

b: 50S ribosomal protein L27

c: 50S ribosomal protein L28

d: 5S rRNA

e: 50S ribosomal protein L29

f: 50S ribosomal protein L30

g: 50S ribosomal protein L31

h: 50S ribosomal protein L2

i: 50S ribosomal protein L32

j: 50S ribosomal protein L3

k: 50S ribosomal protein L33

l: 50S ribosomal protein L4

m: 50S ribosomal protein L34

n: 50S ribosomal protein L5

o: 50S ribosomal protein L35

p: 50S ribosomal protein L6

q: 50S ribosomal protein L36

r: 50S ribosomal protein L9

s: 50S ribosomal protein L13

t: 50S ribosomal protein L14

u: 50S ribosomal protein L15

v: 50S ribosomal protein L16

w: 50S ribosomal protein L17

x: 50S ribosomal protein L18

y: 50S ribosomal protein L19

z: 50S ribosomal protein L20

hetero molecules

2.67 MDa, 66 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author

+
50S ribosomal protein ... , 32 types, 32 molecules 012349YZbcefghijklmnopqrstuvwxyz

#1: Protein	50S ribosomal protein L21 / Mass: 11586.374 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplU / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7ZEN7
#2: Protein	50S ribosomal protein L22 / Mass: 12253.359 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplV / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7Z9G0
#3: Protein	50S ribosomal protein L23 / Mass: 10661.560 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplW / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D6I208
#4: Protein	50S ribosomal protein L24 / Mass: 11208.054 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplX / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7XKZ6
#5: Protein	50S ribosomal protein L25 / Mass: 10713.465 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplY / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7XH79
#9: Protein	50S ribosomal protein L10 / Mass: 15981.518 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplJ / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A3W3FJZ4
#37: Protein	50S ribosomal protein L11 / Mass: 14763.165 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplK / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: S1ERR4
#38: Protein/peptide	50S ribosomal protein L7/L12 / Ribosome Mass: 3300.856 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplL / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A376CUC4
#40: Protein	50S ribosomal protein L27 / Mass: 8275.498 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmA / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A069XRX8
#41: Protein	50S ribosomal protein L28 / Mass: 8896.354 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmB / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: J7RMJ2
#43: Protein	50S ribosomal protein L29 / Mass: 7155.267 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmC / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A069XF36
#44: Protein	50S ribosomal protein L30 / Mass: 6423.625 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmD / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: I2X1N4
#45: Protein	50S ribosomal protein L31 / Mass: 7516.693 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmE / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: V0YYV3
#46: Protein	50S ribosomal protein L2 / / Large ribosomal subunit protein uL2 Mass: 29663.244 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: MRE600 / Gene: rplB / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: P60422
#47: Protein	50S ribosomal protein L32 / Mass: 6332.249 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: H10407 / ETEC / Gene: rpmF / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: E3PJQ3
#48: Protein	50S ribosomal protein L3 / / Large ribosomal subunit protein uL3 Mass: 22277.535 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplC / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: P60438
#49: Protein	50S ribosomal protein L33 / Mass: 6057.179 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmG / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: L4IX08
#50: Protein	50S ribosomal protein L4 / Mass: 22121.566 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplD / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7Z9F6
#51: Protein/peptide	50S ribosomal protein L34 / Mass: 5397.463 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmH / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: B7MGC4
#52: Protein	50S ribosomal protein L5 / Mass: 20073.234 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplE / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: S1EUW9
#53: Protein	50S ribosomal protein L35 / Mass: 7181.835 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmI / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: G0F552
#54: Protein	50S ribosomal protein L6 / Mass: 18672.418 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplF / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A5B7PA13
#55: Protein/peptide	50S ribosomal protein L36 / Mass: 4377.390 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmJ / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A0E2L017
#56: Protein	50S ribosomal protein L9 / Mass: 15789.020 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: MRE600 / Gene: rplI / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7ZI15
#57: Protein	50S ribosomal protein L13 / Mass: 16050.606 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplM / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7ZET0
#58: Protein	50S ribosomal protein L14 / Mass: 13565.067 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: MRE600 / Gene: rplN / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7Z9G5
#59: Protein	50S ribosomal protein L15 / Mass: 15008.471 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplO / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A037Y8L6
#60: Protein	50S ribosomal protein L16 / / Large ribosomal subunit protein uL16 Mass: 15312.269 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplP / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: P0ADY7
#61: Protein	50S ribosomal protein L17 / Mass: 13592.823 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplQ / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A2T1LEK9
#62: Protein	50S ribosomal protein L18 / Mass: 12663.471 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplR / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: F4TL27
#63: Protein	50S ribosomal protein L19 / Mass: 13028.082 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplS / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A4P8C2I6
#64: Protein	50S ribosomal protein L20 / Mass: 13396.828 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplT / Production host: Escherichia coli (E. coli) / References: UniProt: L3CCT0

-
DNA chain , 2 types, 2 molecules 56

#6: DNA chain	NT DNA Mass: 11042.110 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#7: DNA chain	T DNA Mass: 8155.232 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

-
RNA chain , 5 types, 6 molecules 7ABDad

#8: RNA chain	mRNA with 12 nt long spacer Mass: 9238.331 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#10: RNA chain	E-site and A-site tRNA (fMet) Mass: 24496.617 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: GenBank: 1845826323
#16: RNA chain	16S rRNA / Mass: 499690.031 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: GenBank: 1732209707
#39: RNA chain	23S rRNA / 23S ribosomal RNA Mass: 941635.438 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: GenBank: 937521852
#42: RNA chain	5S rRNA / 5S ribosomal RNA Mass: 38790.090 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: GenBank: 1273279017

-
DNA-directed RNA polymerase subunit ... , 3 types, 4 molecules AAACADAE

#11: Protein	DNA-directed RNA polymerase subunit beta / Polymerase / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta Mass: 150820.875 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoB / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain References: UniProt: A0A140SS80, DNA-directed RNA polymerase
#13: Protein	DNA-directed RNA polymerase subunit alpha / Polymerase / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha Mass: 25497.023 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoA / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain References: UniProt: A0A5F0PYH4, DNA-directed RNA polymerase
#14: Protein	DNA-directed RNA polymerase subunit beta' / Polymerase / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta' Mass: 155366.781 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoC / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain References: UniProt: A0A4S1NBU2, DNA-directed RNA polymerase

-
Protein , 1 types, 1 molecules AB

#12: Protein	Transcription termination/antitermination protein NusG Mass: 20560.523 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: nusG / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: U9XYQ6

+
30S ribosomal protein ... , 21 types, 21 molecules CEFGHIJKLMNOPQRSTUVWX

#15: Protein	30S ribosomal protein S18 / Mass: 7734.896 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsR / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A222QU44
#17: Protein	30S ribosomal protein S20 / Mass: 9577.268 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsT / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7X3A7
#18: Protein	30S ribosomal protein S21 / Mass: 8392.844 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsU / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: E9TH65
#19: Protein	30S ribosomal protein S2 / Mass: 25072.867 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsB / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: C3TPN2
#20: Protein	30S ribosomal protein S1 / Mass: 61238.887 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsA / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: B7LDA3
#21: Protein	30S ribosomal protein S3 / Mass: 23248.994 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsC / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A376HTV6
#22: Protein	30S ribosomal protein S4 / Mass: 23383.002 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsD / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: L3PZ69
#23: Protein	30S ribosomal protein S5 / Mass: 16475.037 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsE / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: F4TL26
#24: Protein	30S ribosomal protein S6 / Mass: 12125.993 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsF / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A376ZL25
#25: Protein	30S ribosomal protein S7 / Mass: 16861.523 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsG / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: S1D5F0
#26: Protein	30S ribosomal protein S8 / Mass: 14015.361 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsH / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D8A1L7
#27: Protein	30S ribosomal protein S9 / Mass: 14554.882 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsI / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: T9TH92
#28: Protein	30S ribosomal protein S10 / Mass: 11254.041 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsJ / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A069XEJ0
#29: Protein	30S ribosomal protein S11 / / Small ribosomal subunit protein uS11 Mass: 12487.200 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsK / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: P0A7R9
#30: Protein	30S ribosomal protein S12 / Mass: 13768.157 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsL / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: I2UHF1
#31: Protein	30S ribosomal protein S14 / Mass: 11475.364 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsN / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: I2X0X3
#32: Protein	30S ribosomal protein S15 / Mass: 10159.621 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsO / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: D7XN21
#33: Protein	30S ribosomal protein S16 / Mass: 9207.572 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsP / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: B7MIU7
#34: Protein	30S ribosomal protein S17 / Mass: 9263.946 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsQ / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A080IK26
#35: Protein	30S ribosomal protein S19 / Mass: 9421.018 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsS / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: S1EA57
#36: Protein	30S ribosomal protein S13 / Mass: 12868.091 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsM / Production host: Escherichia coli (E. coli) / Strain (production host): JE28 strain / References: UniProt: A0A4P8BX31

-
Non-polymers , 2 types, 3 molecules

#65: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg
#66: Chemical	ChemComp-ZN / ZINC ION Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn

-
Details

Has ligand of interest	N

-
Experiment

Experiment	Method: ELECTRON MICROSCOPY
EM experiment	Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

Component	Name: Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer Type: COMPLEX / Entity ID: #1-#7, #9-#10, #12-#64 / Source: RECOMBINANT
Molecular weight	Experimental value: NO
Source (natural)	Organism: Escherichia coli (E. coli) / Strain: JE28 strain
Source (recombinant)	Organism: Escherichia coli (E. coli)
Buffer solution	pH: 7.5
Specimen	Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Vitrification	Cryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company
Microscopy	Model: FEI TITAN KRIOS
Electron gun	Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lens	Mode: BRIGHT FIELDBright-field microscopy
Image recording	Electron dose: 45 e/Å² / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

-
Processing

CTF correction	Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstruction	Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 24959 / Symmetry type: POINT

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

Version 3 of the EMDB header file is now the official format.
The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi

Thousand views of thousand structures

Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
Download

-
Validation report

-
Related structure data

-
Links

-
Assembly

-
Components

+
50S ribosomal protein ... , 32 types, 32 molecules 012349YZbcefghijklmnopqrstuvwxyz

-
DNA chain , 2 types, 2 molecules 56

-
RNA chain , 5 types, 6 molecules 7ABDad

-
DNA-directed RNA polymerase subunit ... , 3 types, 4 molecules AAACADAE

-
Protein , 1 types, 1 molecules AB

+
30S ribosomal protein ... , 21 types, 21 molecules CEFGHIJKLMNOPQRSTUVWX

-
Non-polymers , 2 types, 3 molecules

-
Details

-
Experimental details

-
Experiment

-
Sample preparation

-
Electron microscopy imaging

-
Processing

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

-
Aug 12, 2020. Covid-19 info

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi

-Movie

-Structure viewers

+Viewer log

-This page

-This web site

-Options

+Open data

-Basic information

-Structure visualization

-Downloads & links

-Download

-Validation report

-Related structure data

-Links

-Assembly

-Components

+50S ribosomal protein ... , 32 types, 32 molecules 012349YZbcefghijklmnopqrstuvwxyz

-DNA chain , 2 types, 2 molecules 56

-RNA chain , 5 types, 6 molecules 7ABDad

-DNA-directed RNA polymerase subunit ... , 3 types, 4 molecules AAACADAE

-Protein , 1 types, 1 molecules AB

+30S ribosomal protein ... , 21 types, 21 molecules CEFGHIJKLMNOPQRSTUVWX

-Non-polymers , 2 types, 3 molecules

-Details

-Experimental details

-Experiment

-Sample preparation

-Electron microscopy imaging

-Processing

+About Yorodumi

-News

-Feb 9, 2022. New format data for meta-information of EMDB entries

-Aug 12, 2020. Covid-19 info

+Mar 5, 2020. Novel coronavirus structure data

+Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+Jul 12, 2017. Major update of PDB

-Yorodumi

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
Download

-
Validation report

-
Related structure data

-
Links

-
Assembly

-
Components

+
50S ribosomal protein ... , 32 types, 32 molecules 012349YZbcefghijklmnopqrstuvwxyz

-
DNA chain , 2 types, 2 molecules 56

-
RNA chain , 5 types, 6 molecules 7ABDad

-
DNA-directed RNA polymerase subunit ... , 3 types, 4 molecules AAACADAE

-
Protein , 1 types, 1 molecules AB

+
30S ribosomal protein ... , 21 types, 21 molecules CEFGHIJKLMNOPQRSTUVWX

-
Non-polymers , 2 types, 3 molecules

-
Details

-
Experimental details

-
Experiment

-
Sample preparation

-
Electron microscopy imaging

-
Processing

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

-
Aug 12, 2020. Covid-19 info

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi