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- PDB-6ztl: E. coli 70S-RNAP expressome complex in collided state bound to NusG -

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Basic information

Entry
Database: PDB / ID: 6ztl
TitleE. coli 70S-RNAP expressome complex in collided state bound to NusG
Components
  • (30S ribosomal protein ...) x 20
  • (50S ribosomal protein ...) x 28
  • (DNA-directed RNA polymerase subunit ...Polymerase) x 4
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
  • Non-template DNA strand
  • Phe-NH-tRNA(Phe) A-site
  • Template DNA strand
  • Transcription termination/antitermination protein NusG
  • mRNAMessenger RNA
  • tRNA(fmet) P-site
KeywordsGENE REGULATION / Transcription / Translation / Expressome / Ribosome / RNA polymerase
Function / homology
Function and homology information


DNA-templated transcription, elongation / regulation of DNA-templated transcription, elongation / RNA polymerase complex / mRNA binding involved in posttranscriptional gene silencing / ornithine decarboxylase inhibitor activity / misfolded RNA binding / transcription antitermination factor activity, RNA binding / RNA folding / Group I intron splicing / endoribonuclease inhibitor activity ...DNA-templated transcription, elongation / regulation of DNA-templated transcription, elongation / RNA polymerase complex / mRNA binding involved in posttranscriptional gene silencing / ornithine decarboxylase inhibitor activity / misfolded RNA binding / transcription antitermination factor activity, RNA binding / RNA folding / Group I intron splicing / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / negative regulation of endoribonuclease activity / transcription antitermination / translation repressor activity / negative regulation of translational initiation / four-way junction DNA binding / mature ribosome assembly / ribosome assembly / endodeoxyribonuclease activity / translation repressor activity, mRNA regulatory element binding / positive regulation of RNA splicing / polysomal ribosome / DNA-templated transcription, termination / assembly of large subunit precursor of preribosome / positive regulation of translational fidelity / maintenance of translational fidelity / regulation of cell growth / response to reactive oxygen species / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / large ribosomal subunit rRNA binding / cytoplasmic translation / cytosolic large ribosomal subunit / large ribosomal subunit / cytosolic small ribosomal subunit / ribosomal large subunit assembly / regulation of translation / ribosome binding / 5S rRNA binding / response to radiation / small ribosomal subunit / regulation of mRNA stability / protein-containing complex assembly / transferase activity / negative regulation of translation / tRNA binding / ribosome / rRNA binding / structural constituent of ribosome / protein dimerization activity / translation / transcription, DNA-templated / mRNA binding / response to antibiotic / negative regulation of transcription, DNA-templated / magnesium ion binding / RNA binding / DNA binding / zinc ion binding / membrane / cytosol / cytoplasm
Ribosomal protein L2, domain 3 / Translation elongation factor EF1B/ribosomal protein S6 / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / Zinc-binding ribosomal protein / DNA-directed RNA polymerase, alpha subunit / RNA polymerase subunit, RPB6/omega / Nucleic acid-binding, OB-fold / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein L23/L15e core domain superfamily / DNA-directed RNA polymerase, subunit beta-prime ...Ribosomal protein L2, domain 3 / Translation elongation factor EF1B/ribosomal protein S6 / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / Zinc-binding ribosomal protein / DNA-directed RNA polymerase, alpha subunit / RNA polymerase subunit, RPB6/omega / Nucleic acid-binding, OB-fold / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein L23/L15e core domain superfamily / DNA-directed RNA polymerase, subunit beta-prime / 50S ribosomal protein uL4 / Ribosomal protein L25/L23 / Thioredoxin domain / Ribosomal protein S5, N-terminal / Ribosomal protein S5 domain 2-type fold, subgroup / RNA polymerase, alpha subunit, C-terminal / Ribosomal protein L2, domain 2 / Ribosomal protein S6 superfamily / Transcription antitermination protein, NusG, bacteria, conserved site / K homology domain-like, alpha/beta / Ribosomal protein L30, ferredoxin-like fold domain / Ribosomal protein L10e/L16 / Ribosomal protein L30, conserved site / Ribosomal protein S4, conserved site / Ribosomal S11, conserved site / Ribosomal protein S2, conserved site / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein L29, conserved site / DNA-directed RNA polymerase, insert domain / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L21, conserved site / Ribosomal protein L30, bacterial-type / Ribosomal protein S5, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S19, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L15, bacterial-type / Ribosomal protein L20 / Ribosomal protein L13 / Ribosomal protein L13, bacterial-type / KOW / Ribosomal protein L24/L26, conserved site / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S12/S23 / Ribosomal protein S13-like, H2TH / RNA polymerase, subunit omega/K/RPB6 / RNA polymerase, N-terminal / NusG, N-terminal / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / Translation protein SH3-like domain superfamily / Translation protein, beta-barrel domain superfamily / K homology domain superfamily, prokaryotic type / Ribosomal protein L9/RNase H1, N-terminal / S15/NS1, RNA-binding / Ribosomal protein L19, conserved site / Ribosomal protein L22/L17, conserved site / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L4 domain superfamily / Ribosomal protein S19 conserved site / Ribosomal protein L34, conserved site / Ribosomal protein L18e/L15P / Ribosomal protein L35 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L2, C-terminal / Ribosomal protein L2, conserved site / Ribosomal protein S4/S9 / Ribosomal protein L5 domain superfamily / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L13, conserved site / Ribosomal protein S19, superfamily / Ribosomal protein L5, conserved site / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S7 domain / Ribosomal protein S16 domain superfamily / Ribosomal protein L28/L24 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S10 domain / Ribosomal protein L21-like / Ribosomal protein L25 / Ribosomal protein L15 / Ribosomal protein L5, C-terminal / Ribosomal protein L5, N-terminal / L28p-like / Ribosomal protein L5, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein L27, conserved site / Ribosomal protein S17, conserved site / Ribosomal protein L33, conserved site / Ribosomal protein L35, conserved site / Ribosomal protein S10, conserved site / Ribosomal protein S13, conserved site / Ribosomal protein S14, conserved site / Ribosomal protein S18, conserved site / Ribosomal protein S21, conserved site / Ribosomal protein S3, conserved site
50S ribosomal protein L17 / 30S ribosomal protein S14 / 50S ribosomal protein L6 / 50S ribosomal protein L30 / 50S ribosomal protein L21 / 50S ribosomal protein L23 / 30S ribosomal protein S15 / Thioredoxin-like protein 2.1 / 50S ribosomal protein L16 / 50S ribosomal protein L14 ...50S ribosomal protein L17 / 30S ribosomal protein S14 / 50S ribosomal protein L6 / 50S ribosomal protein L30 / 50S ribosomal protein L21 / 50S ribosomal protein L23 / 30S ribosomal protein S15 / Thioredoxin-like protein 2.1 / 50S ribosomal protein L16 / 50S ribosomal protein L14 / 50S ribosomal protein L13 / 50S ribosomal protein L18 / 30S ribosomal protein S17 / gb:cp054236.1: / 50S ribosomal protein L2 / 50S ribosomal protein L3 / 50S ribosomal protein L24 / 50S ribosomal protein L4 / 50S ribosomal protein L22 / 50S ribosomal protein L5 / 30S ribosomal protein S21 / 50S ribosomal protein L25 / 30S ribosomal protein S6 / gb:cp054232.1: / gb:cp047127.1: / gb:cp023165.1: / DNA-directed RNA polymerase subunit omega / DNA-directed RNA polymerase subunit beta' / 50S ribosomal protein L34 / DNA-directed RNA polymerase subunit alpha / 50S ribosomal protein L9 / 50S ribosomal protein L36 / 50S ribosomal protein L15 / Transcription termination/antitermination protein NusG / 30S ribosomal protein S7 / 50S ribosomal protein L19 / 50S ribosomal protein L20 / 50S ribosomal protein L27 / 50S ribosomal protein L28 / 50S ribosomal protein L29 / 50S ribosomal protein L32 / 50S ribosomal protein L33 / 50S ribosomal protein L35 / 30S ribosomal protein S10 / 30S ribosomal protein S9 / 30S ribosomal protein S11 / 30S ribosomal protein S12 / 30S ribosomal protein S13 / 30S ribosomal protein S16 / 30S ribosomal protein S18 / 30S ribosomal protein S19 / 30S ribosomal protein S20 / 30S ribosomal protein S2 / 30S ribosomal protein S3 / 30S ribosomal protein S4 / 30S ribosomal protein S5 / 30S ribosomal protein S8 / gb:cp035706.1:
Biological speciesEscherichia coli (E. coli)
synthetic construct (others)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsWebster, M.W. / Takacs, M. / Weixlbaumer, A.
Funding support France, 5items
OrganizationGrant numberCountry
European Research Council (ERC)679734 France
French Infrastructure for Integrated Structural Biology (FRISBI)ANR-10-INBS-05 France
French National Research AgencyANR-10-LABX-0030-INRT France
French National Research AgencyANR-10-IDEX-0002-02 France
European Molecular Biology Organization (EMBO)LTF375-2019 France
CitationJournal: Science / Year: 2020
Title: Structural basis of transcription-translation coupling and collision in bacteria.
Authors: Michael William Webster / Maria Takacs / Chengjin Zhu / Vita Vidmar / Ayesha Eduljee / Mo'men Abdelkareem / Albert Weixlbaumer /
Abstract: Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The ...Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The basis of expressome assembly and its consequences for transcription and translation are poorly understood. Here, we present a series of structures representing uncoupled, coupled, and collided expressome states determined by cryo-electron microscopy. A bridge between the ribosome and RNAP can be formed by the transcription factor NusG, which stabilizes an otherwise-variable interaction interface. Shortening of the intervening mRNA causes a substantial rearrangement that aligns the ribosome entrance channel to the RNAP exit channel. In this collided complex, NusG linkage is no longer possible. These structures reveal mechanisms of coordination between transcription and translation and provide a framework for future study.
Validation Report
SummaryFull reportAbout validation report
History
DepositionJul 20, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 16, 2020Provider: repository / Type: Initial release
Revision 1.1Sep 23, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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Assembly

Deposited unit
AA: 16S ribosomal RNA
AB: 30S ribosomal protein S2
AC: 30S ribosomal protein S3
AD: 30S ribosomal protein S4
AE: 30S ribosomal protein S5
AF: 30S ribosomal protein S6
AG: 30S ribosomal protein S7
AH: 30S ribosomal protein S8
AI: 30S ribosomal protein S9
AJ: 30S ribosomal protein S10
AK: 30S ribosomal protein S11
AL: 30S ribosomal protein S12
AM: 30S ribosomal protein S13
AN: 30S ribosomal protein S14
AO: 30S ribosomal protein S15
AP: 30S ribosomal protein S16
AQ: 30S ribosomal protein S17
AR: 30S ribosomal protein S18
AS: 30S ribosomal protein S19
AT: 30S ribosomal protein S20
AU: 30S ribosomal protein S21
AV: mRNA
AW: tRNA(fmet) P-site
AX: Phe-NH-tRNA(Phe) A-site
BA: 23S ribosomal RNA
BB: 5S ribosomal RNA
BC: 50S ribosomal protein L2
BD: 50S ribosomal protein L3
BE: 50S ribosomal protein L4
BF: 50S ribosomal protein L5
BG: 50S ribosomal protein L6
BH: 50S ribosomal protein L9
BK: 50S ribosomal protein L13
BL: 50S ribosomal protein L14
BM: 50S ribosomal protein L15
BN: 50S ribosomal protein L16
BO: 50S ribosomal protein L17
BP: 50S ribosomal protein L18
BQ: 50S ribosomal protein L19
BR: 50S ribosomal protein L20
BS: 50S ribosomal protein L21
BT: 50S ribosomal protein L22
BU: 50S ribosomal protein L23
BV: 50S ribosomal protein L24
BW: 50S ribosomal protein L25
BX: 50S ribosomal protein L27
BY: 50S ribosomal protein L28
BZ: 50S ribosomal protein L29
B1: 50S ribosomal protein L30
B2: 50S ribosomal protein L32
B3: 50S ribosomal protein L33
B4: 50S ribosomal protein L34
B5: 50S ribosomal protein L35
B6: 50S ribosomal protein L36
CN: Non-template DNA strand
CT: Template DNA strand
CA: DNA-directed RNA polymerase subunit alpha
CB: DNA-directed RNA polymerase subunit alpha
CC: DNA-directed RNA polymerase subunit beta
CD: DNA-directed RNA polymerase subunit beta'
CE: DNA-directed RNA polymerase subunit omega
CF: Transcription termination/antitermination protein NusG
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,641,526507
Polymers2,630,44662
Non-polymers11,080445
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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RNA chain , 6 types, 6 molecules AAAVAWAXBABB

#1: RNA chain 16S ribosomal RNA /


Mass: 499888.406 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: GenBank: CP054232.1
#22: RNA chain mRNA / Messenger RNA


Mass: 15789.517 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#23: RNA chain tRNA(fmet) P-site


Mass: 24832.918 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: GenBank: CP047127.1
#24: RNA chain Phe-NH-tRNA(Phe) A-site


Mass: 24751.018 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: GenBank: CP054236.1
#25: RNA chain 23S ribosomal RNA /


Mass: 941820.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: GenBank: CP023165.1
#26: RNA chain 5S ribosomal RNA /


Mass: 38790.090 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: GenBank: CP035706.1

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30S ribosomal protein ... , 20 types, 20 molecules ABACADAEAFAGAHAIAJAKALAMANAOAPAQARASATAU

#2: Protein 30S ribosomal protein S2 / / Small ribosomal subunit protein uS2


Mass: 26781.670 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7V0
#3: Protein 30S ribosomal protein S3 / / Small ribosomal subunit protein uS3


Mass: 26031.316 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7V3
#4: Protein 30S ribosomal protein S4 / / Small ribosomal subunit protein uS4


Mass: 23514.199 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7V8
#5: Protein 30S ribosomal protein S5 / / Small ribosomal subunit protein uS5


Mass: 17617.455 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7W1
#6: Protein 30S ribosomal protein S6 /


Mass: 15197.032 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: W8T6F0
#7: Protein 30S ribosomal protein S7 / / Small ribosomal subunit protein uS7


Mass: 17637.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P02359
#8: Protein 30S ribosomal protein S8 / / Small ribosomal subunit protein uS8


Mass: 14146.557 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7W7
#9: Protein 30S ribosomal protein S9 / / Small ribosomal subunit protein uS9


Mass: 14886.270 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7X3
#10: Protein 30S ribosomal protein S10 / / Small ribosomal subunit protein uS10


Mass: 11755.597 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7R5
#11: Protein 30S ribosomal protein S11 / / Small ribosomal subunit protein uS11


Mass: 13870.975 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7R9
#12: Protein 30S ribosomal protein S12 / / Small ribosomal subunit protein uS12


Mass: 13814.249 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7S3
#13: Protein 30S ribosomal protein S13 / / Small ribosomal subunit protein uS13


Mass: 13128.467 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7S9
#14: Protein 30S ribosomal protein S14 / / Small ribosomal subunit protein uS14


Mass: 11606.560 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0AG59
#15: Protein 30S ribosomal protein S15 / / Small ribosomal subunit protein uS15


Mass: 10290.816 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0ADZ4
#16: Protein 30S ribosomal protein S16 / / Small ribosomal subunit protein bS16


Mass: 9207.572 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7T3
#17: Protein 30S ribosomal protein S17 / / Small ribosomal subunit protein uS17


Mass: 9724.491 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0AG63
#18: Protein 30S ribosomal protein S18 / / Small ribosomal subunit protein bS18


Mass: 9005.472 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7T7
#19: Protein 30S ribosomal protein S19 / / Small ribosomal subunit protein uS19


Mass: 10455.355 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7U3
#20: Protein 30S ribosomal protein S20 / / Small ribosomal subunit protein bS20


Mass: 9708.464 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7U7
#21: Protein 30S ribosomal protein S21 / / Small ribosomal subunit protein bS21


Mass: 8524.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P68679

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50S ribosomal protein ... , 28 types, 28 molecules BCBDBEBFBGBHBKBLBMBNBOBPBQBRBSBTBUBVBWBXBYBZB1B2B3B4B5B6

#27: Protein 50S ribosomal protein L2 / / Large ribosomal subunit protein uL2


Mass: 29923.619 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P60422
#28: Protein 50S ribosomal protein L3 / / Large ribosomal subunit protein uL3


Mass: 22291.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P60438
#29: Protein 50S ribosomal protein L4 / / Large ribosomal subunit protein uL4


Mass: 22121.566 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P60723
#30: Protein 50S ribosomal protein L5 / / Large ribosomal subunit protein uL5


Mass: 20333.611 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P62399
#31: Protein 50S ribosomal protein L6 / / Large ribosomal subunit protein uL6


Mass: 18932.791 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0AG55
#32: Protein 50S ribosomal protein L9 / / Large ribosomal subunit protein bL9


Mass: 15789.020 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7R1
#33: Protein 50S ribosomal protein L13 / / Large ribosomal subunit protein uL13


Mass: 16050.606 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0AA10
#34: Protein 50S ribosomal protein L14 / / Large ribosomal subunit protein uL14


Mass: 13565.067 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0ADY3
#35: Protein 50S ribosomal protein L15 /


Mass: 14994.443 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: A0A6D2WI95
#36: Protein 50S ribosomal protein L16 / / Large ribosomal subunit protein uL16


Mass: 15327.261 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0ADY7
#37: Protein 50S ribosomal protein L17 / / Large ribosomal subunit protein bL17


Mass: 14393.657 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0AG44
#38: Protein 50S ribosomal protein L18 / / Large ribosomal subunit protein uL18


Mass: 12794.668 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0C018
#39: Protein 50S ribosomal protein L19 / / Large ribosomal subunit protein bL19


Mass: 13159.278 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7K6
#40: Protein 50S ribosomal protein L20 / / Large ribosomal subunit protein bL20


Mass: 13528.024 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7L3
#41: Protein 50S ribosomal protein L21 / / Large ribosomal subunit protein bL21


Mass: 11586.374 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0AG48
#42: Protein 50S ribosomal protein L22 / / Large ribosomal subunit protein uL22


Mass: 12253.359 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P61175
#43: Protein 50S ribosomal protein L23 / / Large ribosomal subunit protein uL23


Mass: 11222.160 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0ADZ0
#44: Protein 50S ribosomal protein L24 / / Large ribosomal subunit protein uL24


Mass: 11339.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P60624
#45: Protein 50S ribosomal protein L25 / / Large ribosomal subunit protein bL25


Mass: 10713.465 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P68919
#46: Protein 50S ribosomal protein L27 / / Large ribosomal subunit protein bL27


Mass: 9146.540 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7L8
#47: Protein 50S ribosomal protein L28 / / Large ribosomal subunit protein bL28


Mass: 9027.551 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7M2
#48: Protein 50S ribosomal protein L29 / / Large ribosomal subunit protein uL29


Mass: 7286.464 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7M6
#49: Protein 50S ribosomal protein L30 / / Large ribosomal subunit protein uL30


Mass: 6554.820 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0AG51
#50: Protein 50S ribosomal protein L32 / / Large ribosomal subunit protein bL32


Mass: 6463.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7N4
#51: Protein 50S ribosomal protein L33 / / Large ribosomal subunit protein bL33


Mass: 6388.631 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7N9
#52: Protein/peptide 50S ribosomal protein L34 / / Large ribosomal subunit protein bL34


Mass: 5397.463 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7P5
#53: Protein 50S ribosomal protein L35 / / Large ribosomal subunit protein bL35 / Ribosomal protein A


Mass: 7313.032 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7Q1
#54: Protein/peptide 50S ribosomal protein L36 /


Mass: 5720.060 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: A0A2X1PTA3

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DNA chain , 2 types, 2 molecules CNCT

#55: DNA chain Non-template DNA strand


Mass: 12063.754 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#56: DNA chain Template DNA strand


Mass: 11872.596 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules CACBCCCDCE

#57: Protein DNA-directed RNA polymerase subunit alpha / Polymerase / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha


Mass: 36558.680 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoA, pez, phs, sez, b3295, JW3257 / Plasmid: pEcrpoABC(-XH)Z / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0A7Z4, DNA-directed RNA polymerase
#58: Protein DNA-directed RNA polymerase subunit beta / Polymerase / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 150820.875 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950
Plasmid: pEcrpoABC(-XH)Z / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0A8V2, DNA-directed RNA polymerase
#59: Protein DNA-directed RNA polymerase subunit beta' / Polymerase / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta'


Mass: 155366.781 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoC, Z5561, ECs4911 / Plasmid: pEcrpoABC(-XH)Z / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0A8T8, DNA-directed RNA polymerase
#60: Protein DNA-directed RNA polymerase subunit omega / Polymerase / RNAP omega subunit / RNA polymerase omega subunit / Transcriptase subunit omega


Mass: 10249.547 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoZ, b3649, JW3624 / Plasmid: pEcrpoABC(-XH)Z / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0A800, DNA-directed RNA polymerase

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Protein , 1 types, 1 molecules CF

#61: Protein Transcription termination/antitermination protein NusG


Mass: 20560.523 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: nusG, A6592_25585, A6V01_22710, A8C65_22140, A9819_22730, A9P13_25180, A9R57_05240, A9X72_23950, AC067_25565, AC789_1c43770, ACN002_4069, ACN68_05355, ACN81_23140, ACU57_23215, ACU90_26230, ...Gene: nusG, A6592_25585, A6V01_22710, A8C65_22140, A9819_22730, A9P13_25180, A9R57_05240, A9X72_23950, AC067_25565, AC789_1c43770, ACN002_4069, ACN68_05355, ACN81_23140, ACU57_23215, ACU90_26230, AM270_23415, AM446_26285, AM464_13235, AML07_02765, AML35_27155, APT94_08685, APZ14_17375, ARC77_16990, AU473_00875, AUQ13_12310, AUS26_26490, AW059_24245, AW106_22725, AWB10_24980, AWG90_024840, AWP75_07120, B7C53_21495, B9M99_23250, B9T59_11605, BANRA_02652, BANRA_02965, BANRA_04868, BANRA_05054, BB545_03125, BE963_11245, BEN53_24665, BER14_24185, BHF03_13055, BHF46_22020, BHS81_23795, BHS87_22260, BIQ87_22825, BIU72_18845, BIZ41_20425, BJJ90_25150, BK248_06370, BK292_28055, BK296_22625, BK334_23885, BK373_27240, BK375_25285, BK383_27750, BMT91_03315, BN17_39461, BOH76_00570, BON63_20075, BON65_15050, BON66_14345, BON69_07640, BON71_08905, BON72_17240, BON75_02205, BON76_16185, BON83_01585, BON86_02250, BON87_25635, BON92_00555, BON94_22220, BON95_12895, BON96_11035, BTQ04_24460, BTQ06_27300, BUE81_05395, BvCms12BK_01423, BvCms2454_04763, BvCms28BK_04512, BvCms35BK_02101, BvCmsC61A_02144, BvCmsHHP001_05230, BvCmsHHP019_04457, BvCmsHHP056_02613, BvCmsKKP036_03586, BvCmsKKP061_02988, BvCmsKSNP073_02860, BvCmsKSNP081_04473, BvCmsKSNP120_03442, BvCmsKSP011_02762, BvCmsKSP024_02914, BvCmsKSP026_03368, BvCmsKSP045_04193, BvCmsKSP058_05150, BvCmsKSP067_02572, BvCmsKSP076_03394, BvCmsNSNP036_01151, BvCmsNSP006_02609, BvCmsNSP007_05025, BvCmsNSP047_00265, BvCmsNSP072_02887, BvCmsOUP014_03194, BvCmsSINP011_03926, BvCmsSINP022_04149, BvCmsSIP019_00806, BvCmsSIP044_03280, BVL39_05395, BW690_26720, BWI89_17415, BWP17_22740, BXT93_25190, BZL31_04420, BZL69_05760, C2M16_25145, C2U48_12750, C4M78_26060, C5715_03885, C5N07_24575, C5P01_26085, C5P44_24205, C6669_07630, C6B13_24055, C7235_23740, C7B02_25750, C7B06_24590, C7B07_24515, C7B08_25795, C7B18_24885, C9098_22125, C9114_22875, C9141_23580, C9160_23410, C9162_26315, C9201_22170, C9306_18330, C9E25_22050, C9E67_28690, C9Z03_20290, C9Z23_22460, C9Z28_21960, C9Z29_22205, C9Z37_20890, C9Z39_11545, C9Z68_22005, C9Z70_22895, C9Z78_23210, C9Z89_21250, CA593_06055, CDC27_21525, CDL37_18180, CF006_23900, CG692_19490, CI641_013295, CI693_09880, CI694_22430, CIG45_23855, CJU63_23510, CMR93_21685, CO706_19475, COD30_25685, COD46_23680, CQB02_21475, CQP61_26065, CR538_24245, CR539_01640, CRD98_23200, CRE06_24015, CRM83_18660, CRX46_23015, CSB64_25615, CV83915_02071, CVH05_03840, CWM24_27465, CWS33_24585, D0X26_26090, D2184_25125, D2185_24315, D2188_26625, D3821_09130, D3822_07045, D3O91_23455, D3P01_24300, D3Y67_18010, D4M06_24755, D4V08_22465, D6T60_24420, D6X76_25425, D7K63_24265, D7K66_23575, D9D20_23600, D9D31_17400, D9D43_22285, D9E34_21740, D9E35_23840, D9F17_24210, D9G11_22910, D9G42_23575, D9G48_16785, D9H36_23700, D9H53_22650, D9H68_20300, D9H70_22035, D9H94_18920, D9I18_21340, D9I87_21115, D9I88_18975, D9I97_19355, D9J03_21550, D9J11_22250, D9J44_24490, D9J60_24680, D9J78_24465, D9K48_24855, D9K54_12200, D9L89_22830, D9X97_23540, DAH18_24890, DAH26_25975, DAH30_23810, DAH32_23215, DAH34_21590, DAH37_22535, DAH43_24845, DB359_25595, DBQ99_24575, DD762_22315, DEN86_24465, DEN89_26545, DEN97_23745, DEO04_24340, DEO19_24590, DIV22_03710, DJ492_21155, DJ503_21480, DL455_22945, DL545_23715, DL800_27635, DM102_24845, DM129_21405, DMI04_23160, DN627_23120, DN700_22935, DNB37_22335, DND16_22055, DNQ45_05150, DNX30_24180, DOY56_23490, DP258_23400, DP277_21350, DQE83_24500, DQF57_23755, DQO13_23745, DS732_01515, DTL43_13735, DTL90_24565, DTM10_25890, DTM45_24265, DU309_24235, DU321_23455, DWB25_23615, DXT69_22770, DXT71_22275, DXT73_22975, DXX80_014070, E0I42_21930, E0K84_22915, E0L12_22075, E2112_20390, E2115_21525, E2116_21360, E2119_22370, E2127_20870, E2128_03155, E2129_21325, E2134_22760, E2135_19300, E2855_04995, E2863_04087, E4K55_23315, E5P22_12620, E5P28_08855, E5P37_21480, E5S42_21570, E5S46_22920, E5S47_23055, E5S58_21655, E5S61_22405, EA223_18780, EAI42_07555, EAI46_11730, EAI52_22765, EAX79_24725, EB509_19910, EB510_23015, EB515_23215, EC1094V2_4288, EC3234A_76c00020, EC3426_00293, EC382_20035, EC95NR1_03447, ECONIH1_23440, ECs4905, ECTO6_04524, ED225_22815, ED600_17570, ED607_18465, ED611_23905, ED648_18875, ED903_15555, ED944_23285, EEA45_21070, EEP23_09520, EF173_24425, EHH55_00685, EHJ36_22675, EI021_22100, EI028_22550, EI032_21280, EIA08_23380, EIA21_22555, EIZ93_16305, EJC75_19605, EKI52_13975, EL75_4207, EL79_4413, EL80_4320, ELT20_20690, ELT49_22095, ELT58_20800, ELU85_23620, ELV08_18720, ELV15_18010, ELV28_22220, ELY05_18135, EO241_25805, EPT01_21710, EPU41_22430, EQ820_22900, EQ823_21595, EQ825_24495, EQ830_22075, ERL57_22710, ERS085365_04268, ERS085366_03833, ERS085374_04594, ERS085379_04702, ERS085386_04623, ERS085404_04521, ERS085416_04255, ERS139211_00029, ERS150873_04413, ERS150876_04842, EST51_23890, EVY14_16870, EXM29_02430, ExPECSC019_03691, ExPECSC038_04333, EXX06_24555, EXX13_24135, EXX23_22335, EXX24_23200, EXX53_20975, EXX55_24470, EXX71_22285, EXX78_23980, EXX87_25010, EYD11_21620, EYX82_16660, EYY27_22340, EYY34_19835, EYY78_22630, F1E13_23780, F1E19_21855, F7F18_24025, F7F23_23365, F7F29_25900, F7G01_23380, F7G03_24045, FAF34_028990, FKO60_24845, FNJ69_19080, FNJ83_24770, FORC82_4527, FQ915_26180, FQR64_21830, FRV13_16245, FV293_23400, FV295_22235, FV438_22405, FWK02_12320, FY127_17780, FZ043_01110, GJ11_25170, HmCms184_03867, HmCmsJML074_03188, HmCmsJML079_04908, HmCmsJML204_03263, HMPREF3040_01771, HW43_01555, MJ49_09455, MS6198_46010, NCTC10082_02087, NCTC10090_02786, NCTC10429_00094, NCTC10764_02978, NCTC10766_02647, NCTC10865_05901, NCTC10974_05357, NCTC11022_04295, NCTC11112_02275, NCTC11126_05446, NCTC11181_01971, NCTC11341_02768, NCTC12950_05220, NCTC13127_06170, NCTC13148_04401, NCTC13846_04507, NCTC7922_04992, NCTC7927_05255, NCTC8009_08439, NCTC8179_05103, NCTC8450_00357, NCTC8500_05331, NCTC8622_01622, NCTC8960_02346, NCTC9007_05346, NCTC9036_04655, NCTC9044_02298, NCTC9045_05548, NCTC9050_02824, NCTC9055_01673, NCTC9058_02141, NCTC9062_03438, NCTC9077_05942, NCTC9111_04961, NCTC9117_05910, NCTC9119_05061, NCTC9701_05002, NCTC9702_05645, NCTC9703_04220, NCTC9706_02009, NCTC9777_01124, NCTC9969_04938, PGD_00616, PU06_15460, RG28_25315, RK56_017435, RX35_03749, SAMEA3472043_04873, SAMEA3472044_04880, SAMEA3472047_03493, SAMEA3472055_04940, SAMEA3472056_05316, SAMEA3472070_05100, SAMEA3472080_04808, SAMEA3472090_05013, SAMEA3472108_04978, SAMEA3472110_05075, SAMEA3472112_05160, SAMEA3472114_04960, SAMEA3472147_04113, SAMEA3484427_04977, SAMEA3484429_04980, SAMEA3484434_04732, SAMEA3485101_05504, SAMEA3485113_04488, SAMEA3752372_05072, SAMEA3752553_04898, SAMEA3752557_04837, SAMEA3752559_04862, SAMEA3752620_04706, SAMEA3753064_05336, SAMEA3753097_04838, SAMEA3753164_04793, SAMEA3753290_00011, SAMEA3753300_04891, SK85_04313, UC41_25620, UN86_14210, UN91_25475, WQ89_24645, WR15_01460, YDC107_2696
Plasmid: pSKB2_(His)6-HRV3C-NusG / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: C3SID2

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Non-polymers , 3 types, 445 molecules

#62: Chemical...
ChemComp-MG / MAGNESIUM ION / Magnesium


Mass: 24.305 Da / Num. of mol.: 441 / Source method: obtained synthetically / Formula: Mg
#63: Chemical ChemComp-PHE / PHENYLALANINE / Phenylalanine


Type: L-peptide linking / Mass: 165.189 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C9H11NO2
#64: Chemical ChemComp-ZN / ZINC ION / Zinc


Mass: 65.409 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Zn

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Details

Has ligand of interestN

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Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component
IDNameTypeEntity IDParent-IDSource
1E. coli 70S-RNAP expressome complex in collided state bound to NusGCOMPLEX#1-#610MULTIPLE SOURCES
270S ribosome within the collided E. coli expressomeCOMPLEX#1-#21, #25-#541NATURAL
3DNA-directed RNA polymerase within the collided E. coli expressomeCOMPLEX#23-#24, #57-#611RECOMBINANT
4synthetic mRNA and template DNACOMPLEX#22, #55-#561RECOMBINANT
Molecular weight
IDEntity assembly-IDExperimental value
11NO
21NO
31NO
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
12Escherichia coli (E. coli)562
23Escherichia coli (E. coli)562
34synthetic construct (others)32630
Source (recombinant)
IDEntity assembly-IDOrganismNcbi tax-IDPlasmid
13Escherichia coli BL21(DE3) (bacteria)469008pEcrpoABC(-XH)Z
24synthetic construct (others)32630
Buffer solutionpH: 8
Buffer component
IDConc.NameFormulaBuffer-ID
110 mMHEPESHEPES1
2150 mMPotassium acetateKOAc1
35 mMMagneium acetateMg(OAc)21
410 mMAmmonium chlorideNH4Cl1
52 uMZinc chlorideZnCl21
62 mMDithiothreitolDTT1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 90 % / Chamber temperature: 283 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3500 nm / Nominal defocus min: 700 nm
Specimen holderModel: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 42 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k)
Image scansMovie frames/image: 40

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Processing

Software
NameVersionClassificationNB
phenix.real_space_refine1.18.2_3874refinement
PHENIX1.18.2_3874refinement
EM software
IDNameVersionCategory
1RELION3.0.4particle selection
2EPUimage acquisition
4CTFFINDCTF correction
7UCSF Chimeramodel fitting
9PHENIX1.16model refinement
10RELION3.0.4initial Euler assignment
11RELION3.0.4final Euler assignment
13RELION3.0.43D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 684211
3D reconstructionResolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 45774 / Symmetry type: POINT
Atomic model buildingProtocol: RIGID BODY FIT / Space: REAL / Target criteria: Correlation coefficient
Atomic model building
IDPDB-ID3D fitting-ID
14YBB1
26ALH1
RefinementCross valid method: NONE

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