|Title||Structural basis of transcription-translation coupling and collision in bacteria.|
|Journal, issue, pages||Science, Vol. 369, Issue 6509, Page 1355-1359, Year 2020|
|Publish date||Sep 11, 2020|
|Authors||Michael William Webster / Maria Takacs / Chengjin Zhu / Vita Vidmar / Ayesha Eduljee / Mo'men Abdelkareem / Albert Weixlbaumer /|
|PubMed Abstract||Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The ...Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The basis of expressome assembly and its consequences for transcription and translation are poorly understood. Here, we present a series of structures representing uncoupled, coupled, and collided expressome states determined by cryo-electron microscopy. A bridge between the ribosome and RNAP can be formed by the transcription factor NusG, which stabilizes an otherwise-variable interaction interface. Shortening of the intervening mRNA causes a substantial rearrangement that aligns the ribosome entrance channel to the RNAP exit channel. In this collided complex, NusG linkage is no longer possible. These structures reveal mechanisms of coordination between transcription and translation and provide a framework for future study.|
|External links||Science / PubMed:32820062|
|Methods||EM (single particle)|
|Resolution||3 - 3.9 Å|
|Keywords||GENE REGULATION / Transcription / Translation / Expressome / Ribosome / RNA polymerase|
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