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- EMDB-3776: Cryo EM structure of the bacterial disaggregase ClpB (BAP form, D... -

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Basic information

Entry
Database: EMDB / ID: EMD-3776
TitleCryo EM structure of the bacterial disaggregase ClpB (BAP form, DWB mutant), in the ATPgammaS state, bound to the model substrate casein
Map data
Sample
  • Complex: ClpB homo hexamer bound to the model substract casein
    • Complex: ClpB homo hexamer bound to the model substract casein
      • Protein or peptide: ClpB (BAP form, double walker B mutant)
    • Complex: ClpB homo hexamer bound to the model substract casein
      • Protein or peptide: Alpha casein
Function / homology
Function and homology information


endopeptidase Clp complex / ATP-dependent peptidase activity / protein unfolding / cellular response to heat / response to heat / protein refolding / response to oxidative stress / ATP hydrolysis activity / membrane / ATP binding ...endopeptidase Clp complex / ATP-dependent peptidase activity / protein unfolding / cellular response to heat / response to heat / protein refolding / response to oxidative stress / ATP hydrolysis activity / membrane / ATP binding / identical protein binding / cytosol / cytoplasm
Similarity search - Function
ATP-dependent Clp protease ATP-binding subunit ClpA / Chaperonin ClpB / Chaperonins clpA/B signature 2. / ClpA/B, conserved site 2 / Chaperonins clpA/B signature 1. / ClpA/B, conserved site 1 / Clp repeat (R) domain profile. / Clp amino terminal domain, pathogenicity island component / AAA lid domain / ClpA/ClpB, AAA lid domain ...ATP-dependent Clp protease ATP-binding subunit ClpA / Chaperonin ClpB / Chaperonins clpA/B signature 2. / ClpA/B, conserved site 2 / Chaperonins clpA/B signature 1. / ClpA/B, conserved site 1 / Clp repeat (R) domain profile. / Clp amino terminal domain, pathogenicity island component / AAA lid domain / ClpA/ClpB, AAA lid domain / Clp, repeat (R) domain / Clp, N-terminal domain superfamily / ClpA/B family / C-terminal, D2-small domain, of ClpB protein / C-terminal, D2-small domain, of ClpB protein / Clp ATPase, C-terminal / AAA domain (Cdc48 subfamily) / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
ATP-dependent Clp protease ATP-binding subunit ClpA / Chaperone protein ClpB
Similarity search - Component
Biological speciesEscherichia coli (E. coli) / Bos taurus (cattle)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.6 Å
AuthorsDeville C / Carroni M / Franke KB / Topf M / Bukau B / Mogk A / Saibil HR
Funding support United Kingdom, Germany, Sweden, 9 items
OrganizationGrant numberCountry
Wellcome Trust106252 United Kingdom
Wellcome Trust079605 United Kingdom
Wellcome Trust101488 United Kingdom
Medical Research Council (United Kingdom)MR/M019292/1 United Kingdom
Biotechnology and Biological Sciences Research CouncilBB/L014211/1 United Kingdom
Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology Germany
Knut and Alice Wallenberg Foundation and Family Erling Persson Foundation Sweden
German Research FoundationMO 970/4-2 Germany
German Research FoundationBB617/17-2 Germany
CitationJournal: Sci Adv / Year: 2017
Title: Structural pathway of regulated substrate transfer and threading through an Hsp100 disaggregase.
Authors: Célia Deville / Marta Carroni / Kamila B Franke / Maya Topf / Bernd Bukau / Axel Mogk / Helen R Saibil /
Abstract: Refolding aggregated proteins is essential in combating cellular proteotoxic stress. Together with Hsp70, Hsp100 chaperones, including ClpB, form a powerful disaggregation machine that threads ...Refolding aggregated proteins is essential in combating cellular proteotoxic stress. Together with Hsp70, Hsp100 chaperones, including ClpB, form a powerful disaggregation machine that threads aggregated polypeptides through the central pore of tandem adenosine triphosphatase (ATPase) rings. To visualize protein disaggregation, we determined cryo-electron microscopy structures of inactive and substrate-bound ClpB in the presence of adenosine 5'--(3-thiotriphosphate), revealing closed AAA+ rings with a pronounced seam. In the substrate-free state, a marked gradient of resolution, likely corresponding to mobility, spans across the AAA+ rings with a dynamic hotspot at the seam. On the seam side, the coiled-coil regulatory domains are locked in a horizontal, inactive orientation. On the opposite side, the regulatory domains are accessible for Hsp70 binding, substrate targeting, and activation. In the presence of the model substrate casein, the polypeptide threads through the entire pore channel and increased nucleotide occupancy correlates with higher ATPase activity. Substrate-induced domain displacements indicate a pathway of regulated substrate transfer from Hsp70 to the ClpB pore, inside which a spiral of loops contacts the substrate. The seam pore loops undergo marked displacements, along with ordering of the regulatory domains. These asymmetric movements suggest a mechanism for ATPase activation and substrate threading during disaggregation.
History
DepositionJun 22, 2017-
Header (metadata) releaseAug 16, 2017-
Map releaseAug 16, 2017-
UpdateNov 6, 2019-
Current statusNov 6, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.035
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.035
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5ofo
  • Surface level: 0.035
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_3776.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.37 Å/pix.
x 256 pix.
= 350.72 Å
1.37 Å/pix.
x 256 pix.
= 350.72 Å
1.37 Å/pix.
x 256 pix.
= 350.72 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.37 Å
Density
Contour LevelBy AUTHOR: 0.045 / Movie #1: 0.035
Minimum - Maximum-0.06663031 - 0.15679021
Average (Standard dev.)0.00051049935 (±0.0068012965)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 350.72 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.371.371.37
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z350.720350.720350.720
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.0670.1570.001

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Supplemental data

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Mask #1

Fileemd_3776_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: #1

Fileemd_3776_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: #2

Fileemd_3776_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : ClpB homo hexamer bound to the model substract casein

EntireName: ClpB homo hexamer bound to the model substract casein
Components
  • Complex: ClpB homo hexamer bound to the model substract casein
    • Complex: ClpB homo hexamer bound to the model substract casein
      • Protein or peptide: ClpB (BAP form, double walker B mutant)
    • Complex: ClpB homo hexamer bound to the model substract casein
      • Protein or peptide: Alpha casein

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Supramolecule #1: ClpB homo hexamer bound to the model substract casein

SupramoleculeName: ClpB homo hexamer bound to the model substract casein / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 570 KDa

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Supramolecule #2: ClpB homo hexamer bound to the model substract casein

SupramoleculeName: ClpB homo hexamer bound to the model substract casein / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #3: ClpB homo hexamer bound to the model substract casein

SupramoleculeName: ClpB homo hexamer bound to the model substract casein / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2
Source (natural)Organism: Bos taurus (cattle)

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Macromolecule #1: ClpB (BAP form, double walker B mutant)

MacromoleculeName: ClpB (BAP form, double walker B mutant) / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MRLDRLTNKF QLALADAQSL ALGHDNQFIE PLHLMSALLN QEGGSVSPLL TSAGINAGQL RTDINQALNR LPQVEGTGGD VQPSQDLVRV LNLCDKLAQK RGDNFISSEL FVLAALESRG TLADILKAAG ATTANITQAI EQMRGGESVN DQGAEDQRQA LKKYTIDLTE ...String:
MRLDRLTNKF QLALADAQSL ALGHDNQFIE PLHLMSALLN QEGGSVSPLL TSAGINAGQL RTDINQALNR LPQVEGTGGD VQPSQDLVRV LNLCDKLAQK RGDNFISSEL FVLAALESRG TLADILKAAG ATTANITQAI EQMRGGESVN DQGAEDQRQA LKKYTIDLTE RAEQGKLDPV IGRDEEIRRT IQVLQRRTKN NPVLIGEPGV GKTAIVEGLA QRIINGEVPE GLKGRRVLAL DMGALVAGAK YRGEFEERLK GVLNDLAKQE GNVILFIDQL HTMVGAGKAD GAMDAGNMLK PALARGELHC VGATTLDEYR QYIEKDAALE RRFQKVFVAE PSVEDTIAIL RGLKERYELH HHVQITDPAI VAAATLSHRY IADRQLPDKA IDLIDEAASS IRMQIDSKPE ELDRLDRRII QLKLEQQALM KESDEASKKR LDMLNEELSD KERQYSELEE EWKAEKASLS GTQTIKAELE QAKIAIEQAR RVGDLARMSE LQYGKIPELE KQLEAATQLE GKTMRLLRNK VTDAEIAEVL ARWTGIPVSR MMESEREKLL RMEQELHHRV IGQNEAVDAV SNAIRRSRAG LADPNRPIGS FLFLGPTGVG KTELCKALAN FMFDSDEAMV RIDMSEFMEK HSVSRLVGAP PGYVGYEEGG YLTEAVRRRP YSVILLDQVE KAHPDVFNIL LQVLDDGRLT DGQGRTVDFR NTVVIMTSNL GVRETERKSI GLIHQDNSTD AMEEIKKIFR PEFINRIDEV VVFHPLGEQH IASIAQIQLK RLYKRLEERG YEIHISDEAL KLLSENGYDP VYGARPLKRA IQQQIENPLA QQILSGELVP GKVIRLEVNE DRIVAVQH

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Macromolecule #2: Alpha casein

MacromoleculeName: Alpha casein / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: Bos taurus (cattle)
SequenceString: MKLLILTCLV AVALARPKHP IKHQGLPQEV LNENLLRFFV APFPEVFGKE KVNELSKDIG SESTEDQAM EDIKQMEAES ISSSEEIVPN SVEQKHIQKE DVPSERYLGY LEQLLRLKKY K VPQLEIVP NSAEERLHSM KEGIHAQQKE PMIGVNQELA YFYPELFRQF ...String:
MKLLILTCLV AVALARPKHP IKHQGLPQEV LNENLLRFFV APFPEVFGKE KVNELSKDIG SESTEDQAM EDIKQMEAES ISSSEEIVPN SVEQKHIQKE DVPSERYLGY LEQLLRLKKY K VPQLEIVP NSAEERLHSM KEGIHAQQKE PMIGVNQELA YFYPELFRQF YQLDAYPSGA WY YVPLGTQ YTDAPSFSDI PNPIGSENSE KTTMPLW

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.7 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
25.0 mol/LTrisHClTrisHCl
25.0 mol/LNaClSodium chloridesodium chloride
10.0 mol/LMgCl2magnesium chloride
2.0 mol/LATPgS5'-O-(3-thio)triphosphate
1.0 mol/LDTTdithiothreitol
GridModel: Lacey carbon / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: LACEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK III / Details: blot force 0, blot time 3.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 100000
Specialist opticsEnergy filter - Name: GIF
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 4)
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.0)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.0)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.0) / Number images used: 230000
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-5ofo:
Cryo EM structure of the E. coli disaggregase ClpB (BAP form, DWB mutant), in the ATPgammaS state, bound to the model substrate casein

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