- EMDB-3768: Cryo-EM structure of a pre-catalytic human spliceosome primed for... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-3768
タイトル
Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex). Unmasked refinement, sharpened map
マップデータ
sharpened, unmasked refinement. Map was aligned to high resolution map (EMDB 3766) and RESAMPLED from 256 px boxsize to 432 px boxsize in Chimera to fit high-resolution map.
試料
複合体: Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex)
ジャーナル: Cell / 年: 2017 タイトル: Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation. 著者: Karl Bertram / Dmitry E Agafonov / Olexandr Dybkov / David Haselbach / Majety N Leelaram / Cindy L Will / Henning Urlaub / Berthold Kastner / Reinhard Lührmann / Holger Stark / 要旨: Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B ...Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B complex. The U2 snRNP-containing head domain is connected to the B complex main body via three main bridges. U4/U6.U5 tri-snRNP proteins, which are located in the main body, undergo significant rearrangements during tri-snRNP integration into the B complex. These include formation of a partially closed Prp8 conformation that creates, together with Dim1, a 5' splice site (ss) binding pocket, displacement of Sad1, and rearrangement of Brr2 such that it contacts its U4/U6 substrate and is poised for the subsequent spliceosome activation step. The molecular organization of several B-specific proteins suggests that they are involved in negatively regulating Brr2, positioning the U6/5'ss helix, and stabilizing the B complex structure. Our results indicate significant differences between the early activation phase of human and yeast spliceosomes.
ダウンロード / ファイル: emd_3768.map.gz / 形式: CCP4 / 大きさ: 307.5 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
sharpened, unmasked refinement. Map was aligned to high resolution map (EMDB 3766) and RESAMPLED from 256 px boxsize to 432 px boxsize in Chimera to fit high-resolution map.
ボクセルのサイズ
X=Y=Z: 1.16 Å
密度
表面レベル
登録者による: 0.04 / ムービー #1: 0.04
最小 - 最大
-0.06067226 - 0.20512854
平均 (標準偏差)
-0.00024091067 (±0.013150235)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
432
432
432
Spacing
432
432
432
セル
A=B=C: 501.12 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.16
1.16
1.16
M x/y/z
432
432
432
origin x/y/z
0.000
0.000
0.000
length x/y/z
501.120
501.120
501.120
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
432
432
432
D min/max/mean
-0.061
0.205
-0.000
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添付データ
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追加マップ: sharpened, unmasked refinement. Original, not resampled map