|Entry||Database: EMDB / ID: 3764|
|Title||Sub-tomogram average of cytosolic yeast ribosomes associated with ER vesicles|
|Map data||Sub-tomogram average of cytosolic yeast ribosomes bound to ER vesicles|
|Sample||S. cerevisiae cytosolic ribosomes associated with ER vesicles:|
|Source||Saccharomyces cerevisiae (baker's yeast)|
|Method||subtomogram averaging / cryo EM / 44 Å resolution|
|Authors||Gold VAM / Chroscicki P / Bragoszewski P / Chacinska A|
|Citation||Journal: EMBO Rep. / Year: 2017|
Title: Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography.
Authors: Vicki Am Gold / Piotr Chroscicki / Piotr Bragoszewski / Agnieszka Chacinska
Abstract: We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, ...We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.
|Date||Deposition: Jun 14, 2017 / Header (metadata) release: Jun 21, 2017 / Map release: Aug 30, 2017 / Last update: Oct 11, 2017|
|Structure viewer||EM map: |
Downloads & links
|File||emd_3764.map.gz (map file in CCP4 format, 2049 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 6.7 Å|
CCP4 map header:
-Entire S. cerevisiae cytosolic ribosomes associated with ER vesicles
|Entire||Name: S. cerevisiae cytosolic ribosomes associated with ER vesicles|
Number of components: 1
-Component #1: protein, S. cerevisiae cytosolic ribosomes associated with ER vesicles
|Protein||Name: S. cerevisiae cytosolic ribosomes associated with ER vesicles|
Recombinant expression: No
|Source||Species: Saccharomyces cerevisiae (baker's yeast)|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||pH: 7.2|
|Vitrification||Cryogen name: ETHANE|
Details: blotted for ~4 s on one side in a humidified atmosphere
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 2 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD / Defocus: 5000 - 8000 nm|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 (4k x 4k)|
|Processing||Method: subtomogram averaging / Applied symmetry: C1 (asymmetric)|
|3D reconstruction||Software: IMOD / Resolution: 44 Å / Resolution method: FSC 0.5 CUT-OFF|
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