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-Structure paper
Title | Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. |
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Journal, issue, pages | EMBO Rep, Vol. 18, Issue 10, Page 1786-1800, Year 2017 |
Publish date | Aug 21, 2017 |
Authors | Vicki Am Gold / Piotr Chroscicki / Piotr Bragoszewski / Agnieszka Chacinska / |
PubMed Abstract | We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, ...We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria. |
External links | EMBO Rep / PubMed:28827470 / PubMed Central |
Methods | EM (subtomogram averaging) |
Resolution | 38.0 - 46.0 Å |
Structure data | EMDB-3762: EMDB-3763: EMDB-3764: |
Source |
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