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TitleVisualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography.
Journal, issue, pagesEMBO Rep, Vol. 18, Issue 10, Page 1786-1800, Year 2017
Publish dateAug 21, 2017
AuthorsVicki Am Gold / Piotr Chroscicki / Piotr Bragoszewski / Agnieszka Chacinska /
PubMed AbstractWe employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, ...We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.
External linksEMBO Rep / PubMed:28827470 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution38.0 - 46.0 Å
Structure data

EMDB-3762:
Sub-tomogram average of the cytosolic yeast ribosome associated with mitochondria
Method: EM (subtomogram averaging) / Resolution: 38.0 Å

EMDB-3763:
Sub-tomogram average of cytosolic yeast polysomes associated with mitochondria
Method: EM (subtomogram averaging) / Resolution: 46.0 Å

EMDB-3764:
Sub-tomogram average of cytosolic yeast ribosomes associated with ER vesicles
Method: EM (subtomogram averaging) / Resolution: 44.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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