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-Structure paper
タイトル | Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. |
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ジャーナル・号・ページ | EMBO Rep, Vol. 18, Issue 10, Page 1786-1800, Year 2017 |
掲載日 | 2017年8月21日 |
著者 | Vicki Am Gold / Piotr Chroscicki / Piotr Bragoszewski / Agnieszka Chacinska / |
PubMed 要旨 | We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, ...We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria. |
リンク | EMBO Rep / PubMed:28827470 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 38.0 - 46.0 Å |
構造データ | EMDB-3762: EMDB-3763: EMDB-3764: |
由来 |
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