Journal: Sci Rep / Year: 2016 Title: The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes. Authors: Kenta Okamoto / Naoyuki Miyazaki / Daniel S D Larsson / Daisuke Kobayashi / Martin Svenda / Kerstin Mühlig / Filipe R N C Maia / Laura H Gunn / Haruhiko Isawa / Mutsuo Kobayashi / Kyoko ...Authors: Kenta Okamoto / Naoyuki Miyazaki / Daniel S D Larsson / Daisuke Kobayashi / Martin Svenda / Kerstin Mühlig / Filipe R N C Maia / Laura H Gunn / Haruhiko Isawa / Mutsuo Kobayashi / Kyoko Sawabe / Kazuyoshi Murata / Janos Hajdu / Abstract: Omono River virus (OmRV) is a double-stranded RNA virus isolated from Culex mosquitos, and it belongs to a group of unassigned insect viruses that appear to be related to Totiviridae. This paper ...Omono River virus (OmRV) is a double-stranded RNA virus isolated from Culex mosquitos, and it belongs to a group of unassigned insect viruses that appear to be related to Totiviridae. This paper describes electron cryo-microscopy (cryoEM) structures for the intact OmRV virion to 8.9 Å resolution and the structure of the empty virus-like-particle, that lacks RNA, to 8.3 Å resolution. The icosahedral capsid contains 120-subunits and resembles another closely related arthropod-borne totivirus-like virus, the infectious myonecrosis virus (IMNV) from shrimps. Both viruses have an elevated plateau around their icosahedral 5-fold axes, surrounded by a deep canyon. Sequence and structural analysis suggests that this plateau region is mainly composed of the extended C-terminal region of the capsid proteins. In contrast to IMNV, the infectious form of OmRV lacks extensive fibre complexes at its 5-fold axes as directly confirmed by a contrast-enhancement technique, using Zernike phase-contrast cryo-EM. Instead, these fibre complexes are replaced by a short "plug" structure at the five-fold axes of OmRV. OmRV and IMNV have acquired an extracellular phase, and the structures at the five-fold axes may be significant in adaptation to cell-to-cell transmission in metazoan hosts.
History
Deposition
Mar 1, 2016
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Header (metadata) release
Mar 9, 2016
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Map release
Sep 28, 2016
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Update
Sep 28, 2016
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Current status
Sep 28, 2016
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Category: CCD / Film or detector model: TVIPS TEMCAM-F416 (4k x 4k) / Average electron dose: 20 e/Å2
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Image processing
Final reconstruction
Applied symmetry - Point group: I (icosahedral) / Resolution.type: BY AUTHOR / Resolution: 8.3 Å / Resolution method: OTHER / Software - Name: EMAN2, RELION / Number images used: 12207
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