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- EMDB-32756: Cryo-EM structure of SARS-CoV spike receptor-binding domain in co... -
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Open data
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Basic information
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Title | Cryo-EM structure of SARS-CoV spike receptor-binding domain in complex with minke whale ACE2 | |||||||||
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Function / homology | ![]() Maturation of spike protein / Translation of Structural Proteins / Virion Assembly and Release / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Li S / Han P / Qi J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cross-species recognition and molecular basis of SARS-CoV-2 and SARS-CoV binding to ACE2s of marine animals. Authors: Shihua Li / Ruirui Yang / Di Zhang / Pu Han / Zepeng Xu / Qian Chen / Runchu Zhao / Xin Zhao / Xiao Qu / Anqi Zheng / Liang Wang / Linjie Li / Yu Hu / Rong Zhang / Chao Su / Sheng Niu / ...Authors: Shihua Li / Ruirui Yang / Di Zhang / Pu Han / Zepeng Xu / Qian Chen / Runchu Zhao / Xin Zhao / Xiao Qu / Anqi Zheng / Liang Wang / Linjie Li / Yu Hu / Rong Zhang / Chao Su / Sheng Niu / Yanfang Zhang / Jianxun Qi / Kefang Liu / Qihui Wang / George F Gao / ![]() Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has an extremely broad host range that includes hippopotami, which are phylogenetically closely related to whales. The cellular ACE2 ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has an extremely broad host range that includes hippopotami, which are phylogenetically closely related to whales. The cellular ACE2 receptor is one of the key determinants of the host range. Here, we found that ACE2s from several marine mammals and hippopotami could efficiently bind to the receptor-binding domain (RBD) of both SARS-CoV and SARS-CoV-2 and facilitate the transduction of SARS-CoV and SARS-CoV-2 pseudoviruses into ACE2-expressing cells. We further resolved the cryo-electron microscopy complex structures of the minke whale ACE2 and sea lion ACE2, respectively, bound to the RBDs, revealing that they have similar binding modes to human ACE2 when it comes to the SARS-CoV-2 RBD and SARS-CoV RBD. Our results indicate that marine mammals could potentially be new victims or virus carriers of SARS-CoV-2, which deserves further careful investigation and study. It will provide an early warning for the prospective monitoring of marine mammals. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 167.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.4 KB 13.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.5 KB | Display | ![]() |
Images | ![]() | 80.4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7wsfMC ![]() 7wseC ![]() 7wsgC ![]() 7wshC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.669 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : SARS-CoV spike receptor-binding domain in complex with minke whal...
Entire | Name: SARS-CoV spike receptor-binding domain in complex with minke whale ACE2 |
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Components |
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-Supramolecule #1: SARS-CoV spike receptor-binding domain in complex with minke whal...
Supramolecule | Name: SARS-CoV spike receptor-binding domain in complex with minke whale ACE2 type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() ![]() ![]() |
-Supramolecule #2: minke whale ACE2
Supramolecule | Name: minke whale ACE2 / type: complex / Chimera: Yes / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Supramolecule #3: SARS-CoV spike receptor-binding domain
Supramolecule | Name: SARS-CoV spike receptor-binding domain / type: complex / Chimera: Yes / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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-Macromolecule #1: Angiotensin-converting enzyme
Macromolecule | Name: Angiotensin-converting enzyme / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: ![]() |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 85.789805 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MSGSFWLLLS LVAVTAAQST TEEQAKTFLQ KFDHEAEDLS YRSSLASWNY NTNITDENVQ KMNAARAKWS AFYEEQSRIA KTYPLEEIQ NLTLKRQLQA LQQSGTSVLS ADKSKRLNTI LNTMSTIYSS GKVLDPNTQE YLVLEPGLDD IMENSEDYNR R LWAWEGWR ...String: MSGSFWLLLS LVAVTAAQST TEEQAKTFLQ KFDHEAEDLS YRSSLASWNY NTNITDENVQ KMNAARAKWS AFYEEQSRIA KTYPLEEIQ NLTLKRQLQA LQQSGTSVLS ADKSKRLNTI LNTMSTIYSS GKVLDPNTQE YLVLEPGLDD IMENSEDYNR R LWAWEGWR AEVGKQLRPF YEEYVVLENE MARANNYEDY GDYWRGDYEV TGADGYDYSR NQLIADVERT FAEIKPLYEQ LH AYVRAKL MDAYPSRISP TGCLPAHLLG DMWGRFWTNL YPLTVPFGEK PSIDVTKEMQ NQSWDAKRIF KEAEKFFVSI GLP NMTQEF WVNSMLTEPG DGRKVVCHPT AWDLGKGDFR IKMCTKVTMD DFLTAHHEMG HIQYDMAYAT QPFLLRNGAN EGFH EAVGE IMSLSAATPH YLKALGLLPP DFYEDNVTEI NFLLKQALQI VGTLPFTYML EKWRWMVFKG EIPKEQWMQK WWEMK REIV GVVEPLPHDE TYCDPACLFH VAEDYSFIRY YTRTIYQFQF HEALCQTAKH EGPLYKCDIS NSTEAGQRLL QMLHLG KSE PWTLALENIV GVKTMDVKPL LNYFEPLLTW LKEQNRNSPV GWSTDWTPYS DQSIKVRISL KSALGEKAYE WNDNEMY LF QSSVAYAMRE YFSKVRNETI PFGEKDVWVS DLKPRISFNF FVTTPKNVSD IIPRTEVEEA IRMSRGRIND AFRLDDNS L EFLGIQPTLG PPYEPPVT |
-Macromolecule #2: Spike protein S1
Macromolecule | Name: Spike protein S1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 21.624326 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: NLCPFGEVFN ATKFPSVYAW ERKKISNCVA DYSVLYNSTF FSTFKCYGVS ATKLNDLCFS NVYADSFVVK GDDVRQIAPG QTGVIADYN YKLPDDFMGC VLAWNTRNID ATSTGNYNYK YRYLRHGKLR PFERDISNVP FSPDGKPCTP PALNCYWPLN D YGFYTTTG IGYQPYRVVV LSFEGSLEVL FQ |
-Macromolecule #3: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 3 / Number of copies: 1 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |