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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30615 | |||||||||
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Title | human potassium-chloride co-transporter KCC2 | |||||||||
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![]() | potassium-chloride / co-transporter / TRANSPORT PROTEIN | |||||||||
Function / homology | ![]() thermosensory behavior / intracellular pH reduction / hypotonic response / potassium:chloride symporter activity / Cation-coupled Chloride cotransporters / intracellular chloride ion homeostasis / ammonium channel activity / chloride ion homeostasis / dendritic spine development / postsynaptic neurotransmitter receptor diffusion trapping ...thermosensory behavior / intracellular pH reduction / hypotonic response / potassium:chloride symporter activity / Cation-coupled Chloride cotransporters / intracellular chloride ion homeostasis / ammonium channel activity / chloride ion homeostasis / dendritic spine development / postsynaptic neurotransmitter receptor diffusion trapping / potassium ion homeostasis / cell volume homeostasis / chloride transmembrane transporter activity / postsynaptic specialization membrane / potassium ion import across plasma membrane / regulation of postsynapse assembly / monoatomic ion transport / dendrite membrane / chloride transmembrane transport / cell periphery / learning / multicellular organism growth / chemical synaptic transmission / perikaryon / neuron projection / response to xenobiotic stimulus / neuronal cell body / protein kinase binding / glutamatergic synapse / metal ion binding / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
![]() | Xie Y / Chang S | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structures and an activation mechanism of human potassium-chloride cotransporters. Authors: Yuan Xie / Shenghai Chang / Cheng Zhao / Feng Wang / Si Liu / Jin Wang / Eric Delpire / Sheng Ye / Jiangtao Guo / ![]() ![]() Abstract: Potassium-chloride cotransporters KCC1 to KCC4 mediate the coupled export of potassium and chloride across the plasma membrane and play important roles in cell volume regulation, auditory system ...Potassium-chloride cotransporters KCC1 to KCC4 mediate the coupled export of potassium and chloride across the plasma membrane and play important roles in cell volume regulation, auditory system function, and γ-aminobutyric acid (GABA) and glycine-mediated inhibitory neurotransmission. Here, we present 2.9- to 3.6-Å resolution structures of full-length human KCC2, KCC3, and KCC4. All three KCCs adopt a similar overall architecture, a domain-swap dimeric assembly, and an inward-facing conformation. The structural and functional studies reveal that one unexpected N-terminal peptide binds at the cytosolic facing cavity and locks KCC2 and KCC4 at an autoinhibition state. The C-terminal domain (CTD) directly interacts with the N-terminal inhibitory peptide, and the relative motions between the CTD and the transmembrane domain (TMD) suggest that CTD regulates KCCs' activities by adjusting the autoinhibitory effect. These structures provide the first glimpse of full-length structures of KCCs and an autoinhibition mechanism among the amino acid-polyamine-organocation transporter superfamily. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 41.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.1 KB 12.1 KB | Display Display | ![]() |
Images | ![]() | 64.3 KB | ||
Filedesc metadata | ![]() | 6.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 568 KB | Display | ![]() |
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Full document | ![]() | 567.5 KB | Display | |
Data in XML | ![]() | 5.9 KB | Display | |
Data in CIF | ![]() | 6.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7d8zMC ![]() 7d90C ![]() 7d99C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.014 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : potassium-chloride cotransporter 2
Entire | Name: potassium-chloride cotransporter 2 |
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Components |
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-Supramolecule #1: potassium-chloride cotransporter 2
Supramolecule | Name: potassium-chloride cotransporter 2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: potassium-chloride cotransporter 2
Macromolecule | Name: potassium-chloride cotransporter 2 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 128.486414 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MDYKDDDDKM SRRFTVTSLP PAGPARSPDP ESRRHSVADP RHLPGEDVKG DGNPKESSPF INSTDTEKGK EYDGKNMALF EEEMDTSPM VSSLLSGLAN YTNLPQGSRE HEEAENNEGG KKKPVQAPRM GTFMGVYLPC LQNIFGVILF LRLTWVVGIA G IMESFCMV ...String: MDYKDDDDKM SRRFTVTSLP PAGPARSPDP ESRRHSVADP RHLPGEDVKG DGNPKESSPF INSTDTEKGK EYDGKNMALF EEEMDTSPM VSSLLSGLAN YTNLPQGSRE HEEAENNEGG KKKPVQAPRM GTFMGVYLPC LQNIFGVILF LRLTWVVGIA G IMESFCMV FICCSCTMLT AISMSAIATN GVVPAGGSYY MISRSLGPEF GGAVGLCFYL GTTFAGAMYI LGTIEILLAY LF PAMAIFK AEDASGEAAA MLNNMRVYGT CVLTCMATVV FVGVKYVNKF ALVFLGCVIL SILAIYAGVI KSAFDPPNFP ICL LGNRTL SRHGFDVCAK LAWEGNETVT TRLWGLFCSS RFLNATCDEY FTRNNVTEIQ GIPGAASGLI KENLWSSYLT KGVI VERSG MTSVGLADGT PIDMDHPYVF SDMTSYFTLL VGIYFPSVTG IMAGSNRSGD LRDAQKSIPT GTILAIATTS AVYIS SVVL FGACIEGVVL RDKFGEAVNG NLVVGTLAWP SPWVIVIGSF FSTCGAGLQS LTGAPRLLQA ISRDGIVPFL QVFGHG KAN GEPTWALLLT ACICEIGILI ASLDEVAPIL SMFFLMCYMF VNLACAVQTL LRTPNWRPRF RYYHWTLSFL GMSLCLA LM FICSWYYALV AMLIAGLIYK YIEYRGAEKE WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLVRV DQDQNVVH P QLLSLTSQLK AGKGLTIVGS VLEGTFLENH PQAQRAEESI RRLMEAEKVK GFCQVVISSN LRDGVSHLIQ SGGLGGLQH NTVLVGWPRN WRQKEDHQTW RNFIELVRET TAGHLALLVT KNVSMFPGNP ERFSEGSIDV WWIVHDGGML MLLPFLLRHH KVWRKCKMR IFTVAQMDDN SIQMKKDLTT FLYHLRITAE VEVVEMHESD ISAYTYEKTL VMEQRSQILK QMHLTKNERE R EIQSITDE SRGSIRRKNP ANTRLRLNVP EETAGDSEEK PEEEVQLIHD QSAPSCPSSS PSPGEEPEGE GETDPEKVHL TW TKDKSVA EKNKGPSPVS SEGIKDFFSM KPEWENLNQS NVRRMHTAVR LNEVIVKKSR DAKLVLLNMP GPPRNRNGDE NYM EFLEVL TEHLDRVMLV RGGGREVITI YSWSHPQFEK UniProtKB: Solute carrier family 12 member 5 |
-Macromolecule #3: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 3 / Number of copies: 4 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 62.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |