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- EMDB-30461: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30461 | ||||||||||||
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Title | cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 | ||||||||||||
![]() | cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 | ||||||||||||
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Biological species | ![]() ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.3 Å | ||||||||||||
![]() | Guo L / Bi WW / Zhang YY / Yan RH / Li YN / Zhou Q / Dang BB | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Engineered trimeric ACE2 binds viral spike protein and locks it in "Three-up" conformation to potently inhibit SARS-CoV-2 infection. Authors: Liang Guo / Wenwen Bi / Xinling Wang / Wei Xu / Renhong Yan / Yuanyuan Zhang / Kai Zhao / Yaning Li / Mingfeng Zhang / Xia Cai / Shibo Jiang / Youhua Xie / Qiang Zhou / Lu Lu / Bobo Dang / ![]() | ||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 83.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.7 KB 10.7 KB | Display Display | ![]() |
Images | ![]() | 34.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 337.5 KB | Display | ![]() |
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Full document | ![]() | 337 KB | Display | |
Data in XML | ![]() | 6.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.087 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2
Entire | Name: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 |
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Components |
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-Supramolecule #1: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2
Supramolecule | Name: cryo EM map of the interface between RBD of SARS-CoV-2 and T-ACE2 type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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-Supramolecule #2: SARS-CoV-2
Supramolecule | Name: SARS-CoV-2 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() |
-Supramolecule #3: T-ACE2
Supramolecule | Name: T-ACE2 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.6) / Number images used: 59822 |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |