National Natural Science Foundation of China (NSFC)
31521002
China
National Natural Science Foundation of China (NSFC)
31700721
China
National Natural Science Foundation of China (NSFC)
31825009
China
Ministry of Science and Technology (MoST, China)
2016YFA0501100
China
Beijing Municipal Science and Technology Project
Z171100000417001
China
Chinese Academy of Sciences
2018125
China
Citation
Journal: Mol Cell / Year: 2020 Title: Structural Basis for pri-miRNA Recognition by Drosha. Authors: Wenxing Jin / Jia Wang / Chao-Pei Liu / Hong-Wei Wang / Rui-Ming Xu / Abstract: A commencing and critical step in miRNA biogenesis involves processing of pri-miRNAs in the nucleus by Microprocessor. An important, but not completely understood, question is how Drosha, the ...A commencing and critical step in miRNA biogenesis involves processing of pri-miRNAs in the nucleus by Microprocessor. An important, but not completely understood, question is how Drosha, the catalytic subunit of Microprocessor, binds pri-miRNAs and correctly specifies cleavage sites. Here we report the cryoelectron microscopy structures of the Drosha-DGCR8 complex with and without a pri-miRNA. The RNA-bound structure provides direct visualization of the tertiary structure of pri-miRNA and shows that a helix hairpin in the extended PAZ domain and the mobile basic (MB) helix in the RNase IIIa domain of Drosha coordinate to recognize the single-stranded to double-stranded junction of RNA, whereas the dsRNA binding domain makes extensive contacts with the RNA stem. Furthermore, the RNA-free structure reveals an autoinhibitory conformation of the PAZ helix hairpin. These findings provide mechanistic insights into pri-miRNA cleavage site selection and conformational dynamics governing pri-miRNA recognition by the catalytic component of Microprocessor.
History
Deposition
Feb 10, 2020
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Header (metadata) release
Apr 15, 2020
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Map release
Apr 15, 2020
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Update
Mar 27, 2024
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Current status
Mar 27, 2024
Processing site: PDBj / Status: Released
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